AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049943

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049943.t1 MTR_5g080150 55.181 415 142 6 1 407 1 379 1.90e-140 408
MS.gene049943.t1 MTR_2g048140 39.912 456 260 8 6 454 7 455 2.64e-100 308
MS.gene049943.t1 MTR_1g090907 39.780 455 261 7 6 454 7 454 8.32e-99 304
MS.gene049943.t1 MTR_3g448340 36.538 468 271 12 1 452 1 458 6.13e-83 263
MS.gene049943.t1 MTR_1g090903 32.969 458 248 11 4 454 5 410 8.67e-63 209
MS.gene049943.t1 MTR_7g029260 61.111 126 49 0 302 427 34 159 9.77e-45 156
MS.gene049943.t1 MTR_3g049530 32.961 358 180 11 134 455 84 417 1.70e-40 150
MS.gene049943.t1 MTR_5g066890 70.930 86 23 1 317 402 1 84 1.17e-32 119
MS.gene049943.t1 MTR_5g066900 74.747 99 10 1 1 99 1 84 7.35e-32 117
MS.gene049943.t1 MTR_5g080160 51.515 99 42 1 362 454 61 159 1.77e-24 99.8
MS.gene049943.t1 MTR_5g080160 70.175 57 16 1 1 56 1 57 2.07e-14 71.2
MS.gene049943.t1 MTR_2g047858 25.234 428 268 13 6 421 115 502 6.39e-22 99.0
MS.gene049943.t1 MTR_5g092960 24.262 474 305 16 6 455 19 462 3.92e-18 87.0
MS.gene049943.t1 MTR_5g080110 61.194 67 26 0 1 67 1 67 8.63e-18 77.8
MS.gene049943.t1 MTR_1g021825 23.718 468 274 18 6 452 17 422 3.55e-17 84.0
MS.gene049943.t1 MTR_1g021825 23.718 468 274 18 6 452 88 493 4.56e-17 84.0
MS.gene049943.t1 MTR_8g468470 26.906 446 274 20 4 422 12 432 2.33e-16 81.6
MS.gene049943.t1 MTR_2g072720 25.214 468 273 19 5 441 38 459 7.37e-16 80.1
MS.gene049943.t1 MTR_1g016340 23.990 421 283 12 6 419 15 405 7.68e-16 79.7
MS.gene049943.t1 MTR_1g016340 23.990 421 283 12 6 419 20 410 8.98e-16 79.7
MS.gene049943.t1 MTR_8g467800 24.263 441 276 15 5 422 19 424 7.30e-15 77.0
MS.gene049943.t1 MTR_5g080090 49.333 75 28 2 331 404 39 104 9.48e-15 70.9
MS.gene049943.t1 MTR_1g013840 23.756 442 271 16 1 423 51 445 1.80e-14 75.9
MS.gene049943.t1 MTR_4g032730 25.105 478 289 19 6 447 15 459 5.14e-14 74.3
MS.gene049943.t1 MTR_8g468810 23.629 474 303 15 3 452 22 460 1.03e-13 73.2
MS.gene049943.t1 MTR_5g084070 23.620 453 293 11 6 449 13 421 1.35e-13 72.8
MS.gene049943.t1 MTR_2g047872 21.596 426 283 11 6 421 23 407 2.14e-13 72.4
MS.gene049943.t1 MTR_4g045900 22.176 478 287 19 6 421 26 480 3.68e-13 71.6
MS.gene049943.t1 MTR_4g103670 23.235 439 254 18 6 422 25 402 8.19e-13 70.5
MS.gene049943.t1 MTR_8g014160 31.188 202 122 9 6 197 41 235 8.70e-13 70.1
MS.gene049943.t1 MTR_1g011400 21.748 469 290 14 6 452 7 420 1.40e-12 69.7
MS.gene049943.t1 MTR_5g040850 23.419 427 277 13 3 421 4 388 1.81e-12 69.3
MS.gene049943.t1 MTR_2g072730 23.656 465 283 18 6 441 16 437 1.93e-12 69.3
MS.gene049943.t1 MTR_0049s0110 28.205 195 127 6 6 199 8 190 2.63e-12 67.4
MS.gene049943.t1 MTR_0049s0110 28.205 195 127 6 6 199 8 190 2.81e-12 68.2
MS.gene049943.t1 MTR_2g091145 23.185 427 274 15 6 419 21 406 2.96e-12 68.6
MS.gene049943.t1 MTR_0049s0110 28.205 195 127 6 6 199 8 190 3.02e-12 68.2
MS.gene049943.t1 MTR_0049s0110 22.440 459 293 15 6 452 8 415 3.55e-12 68.6
MS.gene049943.t1 MTR_4g080590 23.148 432 281 15 6 421 23 419 4.25e-12 68.2
MS.gene049943.t1 MTR_5g080120 55.556 54 24 0 317 370 1 54 9.17e-12 60.5
MS.gene049943.t1 MTR_8g062010 25.751 466 274 23 4 447 7 422 1.25e-11 66.6
MS.gene049943.t1 MTR_2g047848 23.148 432 271 17 5 419 6 393 1.45e-11 66.6
MS.gene049943.t1 MTR_8g062080 24.528 477 277 22 4 452 21 442 2.34e-11 65.9
MS.gene049943.t1 MTR_7g076760 23.529 442 264 18 6 419 21 416 2.37e-11 65.9
MS.gene049943.t1 MTR_5g092960 24.242 363 240 11 6 349 19 365 3.45e-11 65.1
MS.gene049943.t1 MTR_8g062270 24.295 461 300 19 6 447 16 446 3.96e-11 65.1
MS.gene049943.t1 MTR_8g445780 24.221 417 268 16 6 410 13 393 4.39e-11 64.7
MS.gene049943.t1 MTR_1g071060 38.636 88 53 1 38 125 7 93 5.63e-11 59.3
MS.gene049943.t1 MTR_4g088845 22.454 432 278 14 6 419 19 411 5.94e-11 64.7
MS.gene049943.t1 MTR_8g062310 25.486 463 265 19 2 447 21 420 6.10e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049943.t1 AT1G56400 33.100 429 261 9 2 414 9 427 6.39e-66 219
MS.gene049943.t1 AT3G62230 31.163 430 278 7 2 421 4 425 8.52e-60 202
MS.gene049943.t1 AT5G54820 30.841 428 266 8 2 410 3 419 9.99e-59 200
MS.gene049943.t1 AT3G29830 29.274 468 300 12 6 455 8 462 5.97e-54 187
MS.gene049943.t1 AT1G56400 31.469 429 227 10 2 414 9 386 1.36e-53 186
MS.gene049943.t1 AT3G29830 29.424 469 297 13 6 455 8 461 2.56e-53 186
MS.gene049943.t1 AT2G17305 32.143 168 104 3 294 455 106 269 4.41e-24 101
MS.gene049943.t1 AT1G61330 25.114 438 291 15 7 431 11 424 1.82e-23 102
MS.gene049943.t1 AT3G58880 24.528 424 279 13 6 409 3 405 4.29e-18 86.7
MS.gene049943.t1 AT3G58860 24.292 424 291 12 6 421 7 408 1.02e-16 82.8
MS.gene049943.t1 AT4G26340 23.884 448 301 15 6 449 2 413 1.44e-16 82.0
MS.gene049943.t1 AT1G16930 22.526 475 301 12 4 452 13 446 4.30e-16 80.5
MS.gene049943.t1 AT1G16930 22.526 475 301 12 4 452 13 446 4.30e-16 80.5
MS.gene049943.t1 AT1G16930 22.526 475 301 12 4 452 13 446 4.30e-16 80.5
MS.gene049943.t1 AT4G03220 23.887 494 313 19 6 455 24 498 6.16e-16 80.5
MS.gene049943.t1 AT3G28410 21.909 461 318 11 6 454 28 458 1.02e-15 79.7
MS.gene049943.t1 AT1G61320 22.426 437 299 14 10 431 25 436 1.03e-15 79.7
MS.gene049943.t1 AT5G53635 24.201 438 276 14 7 422 1 404 2.59e-15 78.2
MS.gene049943.t1 AT3G52680 24.675 462 299 18 3 452 18 442 3.44e-15 77.8
MS.gene049943.t1 AT3G42770 22.609 460 307 14 8 454 1 424 7.72e-15 77.0
MS.gene049943.t1 AT1G32375 25.754 431 265 17 6 422 2 391 1.01e-14 76.3
MS.gene049943.t1 AT1G32375 25.754 431 265 17 6 422 2 391 1.01e-14 76.3
MS.gene049943.t1 AT5G44980 23.400 453 267 13 3 423 1 405 1.11e-14 76.3
MS.gene049943.t1 AT5G44980 23.400 453 267 13 3 423 1 405 1.11e-14 76.3
MS.gene049943.t1 AT3G58940 25.386 453 276 16 6 434 2 416 1.15e-14 76.6
MS.gene049943.t1 AT3G58940 25.386 453 276 16 6 434 2 416 1.15e-14 76.6
MS.gene049943.t1 AT3G58900 23.690 439 295 11 6 421 2 423 1.95e-14 75.5
MS.gene049943.t1 AT5G22730 21.300 446 286 11 6 423 28 436 3.06e-14 75.1
MS.gene049943.t1 AT5G22730 21.300 446 286 11 6 423 28 436 3.06e-14 75.1
MS.gene049943.t1 AT5G53840 24.520 469 297 16 2 455 14 440 6.66e-14 73.9
MS.gene049943.t1 AT4G26350 23.918 439 261 16 6 418 2 393 8.92e-14 73.6
MS.gene049943.t1 AT1G78760 21.810 431 287 13 6 421 16 411 1.91e-13 72.4
MS.gene049943.t1 AT5G22610 23.871 465 293 17 6 455 18 436 2.60e-13 72.0
MS.gene049943.t1 AT3G59240 21.477 447 296 15 6 423 8 428 4.08e-13 71.6
MS.gene049943.t1 AT1G66310 22.078 462 308 14 6 454 19 441 4.77e-13 71.2
MS.gene049943.t1 AT5G02930 24.332 374 228 11 1 351 23 364 8.50e-13 70.5
MS.gene049943.t1 AT1G80470 22.785 474 291 19 5 454 14 436 1.08e-12 70.1
MS.gene049943.t1 AT5G02910 25.405 370 237 11 6 362 11 354 1.71e-12 69.7
MS.gene049943.t1 AT5G02910 25.210 357 233 10 6 354 11 341 1.72e-12 68.9
MS.gene049943.t1 AT5G02910 25.210 357 233 10 6 354 11 341 1.86e-12 68.9
MS.gene049943.t1 AT3G52680 25.620 363 230 14 3 358 18 347 2.50e-12 68.6
MS.gene049943.t1 AT3G59000 24.670 454 273 16 6 421 2 424 3.74e-12 68.6
MS.gene049943.t1 AT5G22670 22.646 446 279 15 3 423 8 412 4.10e-12 68.2
MS.gene049943.t1 AT3G58920 23.014 491 311 17 6 454 2 467 5.71e-12 68.2
MS.gene049943.t1 AT1G13570 28.500 200 129 6 1 199 1 187 6.22e-12 66.6
MS.gene049943.t1 AT1G69630 23.112 437 280 16 6 421 18 419 1.00e-11 67.0
MS.gene049943.t1 AT4G00160 28.685 251 147 8 3 241 13 243 1.21e-11 67.0
MS.gene049943.t1 AT1G78750 20.293 478 314 15 6 454 18 457 1.32e-11 66.6
MS.gene049943.t1 AT1G78750 20.293 478 314 15 6 454 18 457 1.32e-11 66.6
MS.gene049943.t1 AT4G14103 22.396 384 233 13 4 354 6 357 2.14e-11 66.2
MS.gene049943.t1 AT1G13570 28.631 241 152 10 1 240 1 222 2.40e-11 65.9
MS.gene049943.t1 AT1G13570 28.631 241 152 10 1 240 1 222 2.40e-11 65.9
MS.gene049943.t1 AT4G14103 28.079 203 121 6 4 197 6 192 2.82e-11 65.5
MS.gene049943.t1 AT4G14103 28.079 203 121 6 4 197 6 192 2.82e-11 65.5
MS.gene049943.t1 AT3G44060 21.983 464 307 18 8 454 1 426 5.44e-11 64.7
MS.gene049943.t1 AT4G13985 23.890 473 307 17 2 452 15 456 5.72e-11 64.7
MS.gene049943.t1 AT1G66290 20.386 466 316 18 2 452 25 450 6.27e-11 64.7
MS.gene049943.t1 AT3G58900 26.337 243 170 4 6 245 2 238 6.74e-11 63.9
MS.gene049943.t1 AT3G58900 26.337 243 170 4 6 245 2 238 6.74e-11 63.9
MS.gene049943.t1 AT3G58900 26.337 243 170 4 6 245 2 238 7.59e-11 63.5

Find 60 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAAAGCTTCAAATAATT+TGG 0.116785 5.3:-52640412 MS.gene049943:CDS
CTTTGAGCATCAACAATTTC+AGG 0.151883 5.3:+52643054 None:intergenic
AATCGAGATTAAGAGCTTTA+AGG 0.179493 5.3:-52640571 MS.gene049943:CDS
TCCAAATTATTTGAAGCTTT+TGG 0.187191 5.3:+52640411 None:intergenic
ATTACTTACTTTCTCACTTA+TGG 0.189242 5.3:-52640525 MS.gene049943:CDS
AATTGAAAACCTTTAGATTT+GGG 0.237542 5.3:+52642527 None:intergenic
TTTCAGCTTTCGTGAGAAAA+TGG 0.248575 5.3:-52643023 MS.gene049943:CDS
TTTGGAGATATCAATCTATT+AGG 0.275918 5.3:-52640394 None:intergenic
TAATTGAAAACCTTTAGATT+TGG 0.278932 5.3:+52642526 None:intergenic
ATGTTATCCCAGAGATATAC+TGG 0.291076 5.3:-52640641 MS.gene049943:CDS
AAGAAATGGAATCAAGGATT+TGG 0.294548 5.3:-52642899 MS.gene049943:CDS
AATAAAGCTACATGCTATTA+AGG 0.302871 5.3:-52642704 MS.gene049943:CDS
GAAAATGCTCTACTACTTTA+CGG 0.308720 5.3:+52640442 None:intergenic
ATCGAGATTAAGAGCTTTAA+GGG 0.324791 5.3:-52640570 MS.gene049943:CDS
CTTCTATTCTCTCTAAAGAT+TGG 0.337117 5.3:-52643146 MS.gene049943:CDS
TTTCGTTTCGCTTTAAGAAA+TGG 0.339603 5.3:-52642913 MS.gene049943:CDS
ATACTCCCATAAACTCAAAA+TGG 0.346831 5.3:+52641545 None:intergenic
ATGCTAGGATACATTCGTTT+TGG 0.347569 5.3:+52641604 None:intergenic
CGACATTCCATTCATCTCTA+AGG 0.355244 5.3:+52640472 None:intergenic
AAAATGCTCTACTACTTTAC+GGG 0.368218 5.3:+52640443 None:intergenic
ATTGCCCGAATCATTGCTTT+CGG 0.390421 5.3:-52643208 MS.gene049943:CDS
GAGTCAACCCAGTATATCTC+TGG 0.398444 5.3:+52640633 None:intergenic
TTCTATTCTCTCTAAAGATT+GGG 0.400740 5.3:-52643145 MS.gene049943:CDS
TTTGACCAATTGTGAAAAGT+TGG 0.408885 5.3:-52642677 MS.gene049943:CDS
CGTATGCAGCTACTTGCTTC+AGG 0.420928 5.3:-52642353 MS.gene049943:intron
CGCTTTAAGAAATGGAATCA+AGG 0.433111 5.3:-52642905 MS.gene049943:CDS
TCAATTTCCTTAGAGATGAA+TGG 0.436949 5.3:-52640479 MS.gene049943:CDS
GATGGCCGAAAGCAATGATT+CGG 0.465360 5.3:+52643203 None:intergenic
ATTGTGAGACGCTCTAAGTA+AGG 0.466101 5.3:+52641502 None:intergenic
AGTAAATGCCCCAAATCTAA+AGG 0.472229 5.3:-52642536 MS.gene049943:CDS
TCCCTTGAGCATCGTGGTCA+AGG 0.474795 5.3:-52642433 MS.gene049943:CDS
TGATATTAAATCTCCTGCAT+TGG 0.479200 5.3:-52642476 MS.gene049943:CDS
ATGGCCGAAAGCAATGATTC+GGG 0.488444 5.3:+52643204 None:intergenic
ACTGCTGAAACAATATCATA+AGG 0.492198 5.3:+52642856 None:intergenic
CGCTTCTTTGAACGGCAGAA+AGG 0.500724 5.3:+52643176 None:intergenic
AAAATTTCTTTGGGTTACAT+GGG 0.516495 5.3:-52642727 MS.gene049943:CDS
AAATTGTTGATGCTCAAAGA+AGG 0.517077 5.3:-52643050 MS.gene049943:CDS
TAAATCTCCTGCATTGGATG+AGG 0.519266 5.3:-52642470 MS.gene049943:CDS
CTTTCTGCCGTTCAAAGAAG+CGG 0.527237 5.3:-52643175 MS.gene049943:CDS
TTGAAATGCTGGAATTCGGA+AGG 0.539765 5.3:-52642640 MS.gene049943:CDS
TTACTTTCTCACTTATGGAA+AGG 0.540191 5.3:-52640520 MS.gene049943:CDS
ATTGAAAACCTTTAGATTTG+GGG 0.540504 5.3:+52642528 None:intergenic
TGCAGGAGATTTAATATCAA+CGG 0.548324 5.3:+52642480 None:intergenic
GTCCTTGACCACGATGCTCA+AGG 0.552550 5.3:+52642431 None:intergenic
GTTCTCACCGCTTCTTTGAA+CGG 0.559469 5.3:+52643168 None:intergenic
TATGTCATTGCTCACTCCCA+TGG 0.561333 5.3:+52643239 None:intergenic
GAGCATCGTGGTCAAGGACA+TGG 0.568121 5.3:-52642427 MS.gene049943:CDS
AGATTAACCTCATCCAATGC+AGG 0.570665 5.3:+52642463 None:intergenic
TCCTTGACCACGATGCTCAA+GGG 0.583759 5.3:+52642432 None:intergenic
AGATGCTTAACATTCATGCT+AGG 0.592384 5.3:+52641589 None:intergenic
AGTCAACCCAGTATATCTCT+GGG 0.594219 5.3:+52640634 None:intergenic
AAGGTTATTTATACCGAAGG+AGG 0.595848 5.3:-52641628 MS.gene049943:CDS
TTGTGAGACGCTCTAAGTAA+GGG 0.602951 5.3:+52641503 None:intergenic
CTGAAGCAAGTAGCTGCATA+CGG 0.604279 5.3:+52642354 None:intergenic
GATAAGGTTATTTATACCGA+AGG 0.612007 5.3:-52641631 MS.gene049943:intron
AATTGTTGATGCTCAAAGAA+GGG 0.622812 5.3:-52643049 MS.gene049943:CDS
TGTTATCCCAGAGATATACT+GGG 0.674333 5.3:-52640640 MS.gene049943:CDS
GAACGGCAGAAAGGAAACGA+TGG 0.683061 5.3:+52643185 None:intergenic
AAATGCTCTACTACTTTACG+GGG 0.700305 5.3:+52640444 None:intergenic
AAACTCAAAATGGTGCAAAG+CGG 0.727187 5.3:+52641555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATATAAAATTAAAAACA+TGG - chr5.3:52642922-52642941 MS.gene049943:CDS 10.0%
!!! GTTTTTAATTTTATATCAAA+AGG + chr5.3:52642921-52642940 None:intergenic 10.0%
!! AAAATTTCACACAAATTTAA+TGG + chr5.3:52641652-52641671 None:intergenic 15.0%
!! AATTTAAGAAAAAAGACAAA+AGG + chr5.3:52641318-52641337 None:intergenic 15.0%
!! TAAAGTAATGTTTATAAACT+AGG - chr5.3:52641825-52641844 MS.gene049943:intron 15.0%
!! TATCCTTCAAAAAAAAAAAT+TGG + chr5.3:52641857-52641876 None:intergenic 15.0%
!!! AAAGTAAACTAAAAGTATTT+TGG + chr5.3:52642335-52642354 None:intergenic 15.0%
!!! AATTTTTTTTTTTGAAGGAT+AGG - chr5.3:52641856-52641875 MS.gene049943:intron 15.0%
!!! ATTTTGAATGAAGAATATAT+GGG - chr5.3:52641022-52641041 MS.gene049943:intron 15.0%
!!! GAAAAATATATACCTTTTTA+CGG + chr5.3:52641551-52641570 None:intergenic 15.0%
!!! TATTTTGAATGAAGAATATA+TGG - chr5.3:52641021-52641040 MS.gene049943:intron 15.0%
!!! TTAGTTTACTTTATTTTGTT+GGG - chr5.3:52642343-52642362 MS.gene049943:intron 15.0%
!!! TTTAGTTTACTTTATTTTGT+TGG - chr5.3:52642342-52642361 MS.gene049943:intron 15.0%
!! AAAGACAAAAGGTAAAAAAA+TGG + chr5.3:52641307-52641326 None:intergenic 20.0%
!! AAATAAAACTGCTTAACAAT+TGG + chr5.3:52641888-52641907 None:intergenic 20.0%
!! AATTGAAAACCTTTAGATTT+GGG + chr5.3:52641108-52641127 None:intergenic 20.0%
!! ACAAAAAAAGATGAGTAATA+TGG + chr5.3:52642714-52642733 None:intergenic 20.0%
!! ATCTCTATATTGTATTCATT+GGG - chr5.3:52641469-52641488 MS.gene049943:intron 20.0%
!! GAACAAAATGAATTTCTATT+TGG + chr5.3:52642308-52642327 None:intergenic 20.0%
!! TAATTGAAAACCTTTAGATT+TGG + chr5.3:52641109-52641128 None:intergenic 20.0%
!! TATATATTCAAGTCATGTAA+AGG + chr5.3:52641912-52641931 None:intergenic 20.0%
!! TCACTCAAAAAAATTTCTTT+GGG - chr5.3:52640896-52640915 MS.gene049943:intron 20.0%
!! TTCACTCAAAAAAATTTCTT+TGG - chr5.3:52640895-52640914 MS.gene049943:intron 20.0%
!! TTTATGTAATACTTACAAAC+AGG + chr5.3:52642177-52642196 None:intergenic 20.0%
!!! AGTCCAATTTTTTTTTTTGA+AGG - chr5.3:52641851-52641870 MS.gene049943:intron 20.0%
!!! CTTAGATTTCAAAGTTTTAA+AGG - chr5.3:52641570-52641589 MS.gene049943:CDS 20.0%
!!! GGTTACAAGTATTATTTTTA+TGG - chr5.3:52642536-52642555 MS.gene049943:CDS 20.0%
!!! TAATTGTGAATTTTTGAAGT+AGG - chr5.3:52642796-52642815 MS.gene049943:CDS 20.0%
!!! TATTTTTATGGAGAAATGTT+AGG - chr5.3:52642548-52642567 MS.gene049943:CDS 20.0%
!!! TTGTATTTTTTTTCGTCAAT+GGG - chr5.3:52642377-52642396 MS.gene049943:CDS 20.0%
!!! TTGTTGTTGTTTATATTGTA+GGG - chr5.3:52642951-52642970 MS.gene049943:CDS 20.0%
!!! TTTGTTGTTGTTTATATTGT+AGG - chr5.3:52642950-52642969 MS.gene049943:CDS 20.0%
!!! TTTTATTGTTTTGCTCTTTT+GGG - chr5.3:52641502-52641521 MS.gene049943:CDS 20.0%
!!! TTTTGAATGAAGAATATATG+GGG - chr5.3:52641023-52641042 MS.gene049943:intron 20.0%
!!! TTTTTATTGTTTTGCTCTTT+TGG - chr5.3:52641501-52641520 MS.gene049943:CDS 20.0%
! AATAAAGCTACATGCTATTA+AGG - chr5.3:52640928-52640947 MS.gene049943:intron 25.0%
! ATATATGGGGTTGAAAAAAT+TGG - chr5.3:52641036-52641055 MS.gene049943:intron 25.0%
! ATGAGCTCATTAAAAAATTC+AGG + chr5.3:52640689-52640708 None:intergenic 25.0%
! ATTACTTACTTTCTCACTTA+TGG - chr5.3:52643107-52643126 MS.gene049943:CDS 25.0%
! ATTGAAAACCTTTAGATTTG+GGG + chr5.3:52641107-52641126 None:intergenic 25.0%
! CTACAACCATAAAAATAAGT+AGG + chr5.3:52640812-52640831 None:intergenic 25.0%
! GATCTCTATATTGTATTCAT+TGG - chr5.3:52641468-52641487 MS.gene049943:intron 25.0%
! TCCAAAAGCTTCAAATAATT+TGG - chr5.3:52643220-52643239 MS.gene049943:CDS 25.0%
!! AAAAATTTCTTTGGGTTACA+TGG - chr5.3:52640904-52640923 MS.gene049943:intron 25.0%
!! AAAATTTCTTTGGGTTACAT+GGG - chr5.3:52640905-52640924 MS.gene049943:intron 25.0%
!! CTAAAGGTTTTCAATTATCA+TGG - chr5.3:52641112-52641131 MS.gene049943:intron 25.0%
!! TTCTATTCTCTCTAAAGATT+GGG - chr5.3:52640487-52640506 MS.gene049943:CDS 25.0%
!!! AATACCATAGCTTCTTTTTT+TGG - chr5.3:52642515-52642534 MS.gene049943:CDS 25.0%
!!! ACTTATTTTTATGGTTGTAG+TGG - chr5.3:52640812-52640831 MS.gene049943:intron 25.0%
!!! AGAAAATGGATTGAAAAACA+CGG - chr5.3:52640623-52640642 MS.gene049943:CDS 25.0%
!!! CTTATTTTTATGGTTGTAGT+GGG - chr5.3:52640813-52640832 MS.gene049943:intron 25.0%
!!! CTTGTATTTTTTTTCGTCAA+TGG - chr5.3:52642376-52642395 MS.gene049943:CDS 25.0%
!!! GATTTCAAAGTTTTAAAGGA+TGG - chr5.3:52641574-52641593 MS.gene049943:CDS 25.0%
!!! GTTTTGCCTACTTATTTTTA+TGG - chr5.3:52640803-52640822 MS.gene049943:intron 25.0%
!!! TCCAAATTATTTGAAGCTTT+TGG + chr5.3:52643224-52643243 None:intergenic 25.0%
!!! TTTACTTTATTTTGTTGGGT+TGG - chr5.3:52642347-52642366 MS.gene049943:intron 25.0%
!!! TTTTTTGAAATGCTGGAATT+CGG - chr5.3:52640988-52641007 MS.gene049943:intron 25.0%
AAAATGCTCTACTACTTTAC+GGG + chr5.3:52643192-52643211 None:intergenic 30.0%
AAATTGTTGATGCTCAAAGA+AGG - chr5.3:52640582-52640601 MS.gene049943:CDS 30.0%
AAGAAATGGAATCAAGGATT+TGG - chr5.3:52640733-52640752 MS.gene049943:intron 30.0%
AATCGAGATTAAGAGCTTTA+AGG - chr5.3:52643061-52643080 MS.gene049943:CDS 30.0%
AATTGTTGATGCTCAAAGAA+GGG - chr5.3:52640583-52640602 MS.gene049943:CDS 30.0%
ACTGCTGAAACAATATCATA+AGG + chr5.3:52640779-52640798 None:intergenic 30.0%
ATACTCCCATAAACTCAAAA+TGG + chr5.3:52642090-52642109 None:intergenic 30.0%
ATGTGTGAAAAACTGTTTGA+AGG - chr5.3:52643037-52643056 MS.gene049943:CDS 30.0%
ATTGAGAGTCTAAACTTACT+TGG - chr5.3:52642864-52642883 MS.gene049943:CDS 30.0%
CTGATGAGAGAAAATATAAG+TGG + chr5.3:52642410-52642429 None:intergenic 30.0%
GAAAATGCTCTACTACTTTA+CGG + chr5.3:52643193-52643212 None:intergenic 30.0%
GATAAGGTTATTTATACCGA+AGG - chr5.3:52642001-52642020 MS.gene049943:intron 30.0%
GTAACCAAAAAAAGAAGCTA+TGG + chr5.3:52642522-52642541 None:intergenic 30.0%
TCAATTTCCTTAGAGATGAA+TGG - chr5.3:52643153-52643172 MS.gene049943:CDS 30.0%
TGATATTAAATCTCCTGCAT+TGG - chr5.3:52641156-52641175 MS.gene049943:intron 30.0%
TGCAGGAGATTTAATATCAA+CGG + chr5.3:52641155-52641174 None:intergenic 30.0%
TTACTTTCTCACTTATGGAA+AGG - chr5.3:52643112-52643131 MS.gene049943:CDS 30.0%
TTTGACCAATTGTGAAAAGT+TGG - chr5.3:52640955-52640974 MS.gene049943:intron 30.0%
! ATCGAGATTAAGAGCTTTAA+GGG - chr5.3:52643062-52643081 MS.gene049943:CDS 30.0%
! CTTCTATTCTCTCTAAAGAT+TGG - chr5.3:52640486-52640505 MS.gene049943:CDS 30.0%
! GTTTTGTGTATGATGTGATA+AGG - chr5.3:52641985-52642004 MS.gene049943:intron 30.0%
!! ATGTAGTCATTTTCGTTTTG+AGG - chr5.3:52642261-52642280 MS.gene049943:intron 30.0%
!! CTAACACTCACTTTTCTATT+AGG - chr5.3:52642593-52642612 MS.gene049943:CDS 30.0%
!! TTTCGTTTCGCTTTAAGAAA+TGG - chr5.3:52640719-52640738 MS.gene049943:intron 30.0%
!!! GTTTGAGTTTTTTGAAATGC+TGG - chr5.3:52640981-52641000 MS.gene049943:intron 30.0%
!!! TGTGAATTTTTGAAGTAGGT+AGG - chr5.3:52642800-52642819 MS.gene049943:CDS 30.0%
!!! TTGCACCATTTTGAGTTTAT+GGG - chr5.3:52642082-52642101 MS.gene049943:intron 30.0%
!!! TTTGCACCATTTTGAGTTTA+TGG - chr5.3:52642081-52642100 MS.gene049943:intron 30.0%
!!! TTTTTTTTCGTCAATGGGTT+TGG - chr5.3:52642382-52642401 MS.gene049943:CDS 30.0%
AAACTCAAAATGGTGCAAAG+CGG + chr5.3:52642080-52642099 None:intergenic 35.0%
AAATGCTCTACTACTTTACG+GGG + chr5.3:52643191-52643210 None:intergenic 35.0%
AAGGTTATTTATACCGAAGG+AGG - chr5.3:52642004-52642023 MS.gene049943:intron 35.0%
AGATGCTTAACATTCATGCT+AGG + chr5.3:52642046-52642065 None:intergenic 35.0%
AGTAAATGCCCCAAATCTAA+AGG - chr5.3:52641096-52641115 MS.gene049943:intron 35.0%
ATGTTATCCCAGAGATATAC+TGG - chr5.3:52642991-52643010 MS.gene049943:CDS 35.0%
CTTTGAGCATCAACAATTTC+AGG + chr5.3:52640581-52640600 None:intergenic 35.0%
GGACATGAATAACAACTACA+TGG + chr5.3:52641710-52641729 None:intergenic 35.0%
TGTTATCCCAGAGATATACT+GGG - chr5.3:52642992-52643011 MS.gene049943:CDS 35.0%
TTCTATCGATATCTCTCTCA+AGG + chr5.3:52641731-52641750 None:intergenic 35.0%
TTTCAGCTTTCGTGAGAAAA+TGG - chr5.3:52640609-52640628 MS.gene049943:CDS 35.0%
! ACACTCCAACTTTTCACAAT+TGG + chr5.3:52640963-52640982 None:intergenic 35.0%
! ATGCTAGGATACATTCGTTT+TGG + chr5.3:52642031-52642050 None:intergenic 35.0%
! TTCGTGTATTTTGCTTGGTT+GGG - chr5.3:52641525-52641544 MS.gene049943:CDS 35.0%
!! CGCTTTAAGAAATGGAATCA+AGG - chr5.3:52640727-52640746 MS.gene049943:intron 35.0%
AGATTAACCTCATCCAATGC+AGG + chr5.3:52641172-52641191 None:intergenic 40.0%
AGTCAACCCAGTATATCTCT+GGG + chr5.3:52643001-52643020 None:intergenic 40.0%
ATTGCCCGAATCATTGCTTT+CGG - chr5.3:52640424-52640443 MS.gene049943:CDS 40.0%
CGACATTCCATTCATCTCTA+AGG + chr5.3:52643163-52643182 None:intergenic 40.0%
TAAATCTCCTGCATTGGATG+AGG - chr5.3:52641162-52641181 MS.gene049943:intron 40.0%
TGAGATCCATTTCCAATGTG+TGG - chr5.3:52642646-52642665 MS.gene049943:CDS 40.0%
TTGAAATGCTGGAATTCGGA+AGG - chr5.3:52640992-52641011 MS.gene049943:intron 40.0%
! ATTGTGAGACGCTCTAAGTA+AGG + chr5.3:52642133-52642152 None:intergenic 40.0%
! GTTCGTGTATTTTGCTTGGT+TGG - chr5.3:52641524-52641543 MS.gene049943:CDS 40.0%
! TTGGGTTCGTGTATTTTGCT+TGG - chr5.3:52641520-52641539 MS.gene049943:CDS 40.0%
! TTGTGAGACGCTCTAAGTAA+GGG + chr5.3:52642132-52642151 None:intergenic 40.0%
ATGGCCGAAAGCAATGATTC+GGG + chr5.3:52640431-52640450 None:intergenic 45.0%
CTGAAGCAAGTAGCTGCATA+CGG + chr5.3:52641281-52641300 None:intergenic 45.0%
CTTTCTGCCGTTCAAAGAAG+CGG - chr5.3:52640457-52640476 MS.gene049943:CDS 45.0%
GAGTCAACCCAGTATATCTC+TGG + chr5.3:52643002-52643021 None:intergenic 45.0%
GATGGCCGAAAGCAATGATT+CGG + chr5.3:52640432-52640451 None:intergenic 45.0%
GTTCTCACCGCTTCTTTGAA+CGG + chr5.3:52640467-52640486 None:intergenic 45.0%
TGGGTACCACACATTGGAAA+TGG + chr5.3:52642655-52642674 None:intergenic 45.0%
!! CATTCGTTTTGGTCCTCCTT+CGG + chr5.3:52642020-52642039 None:intergenic 45.0%
ACTCTCTCTAACACTCGCCT+GGG + chr5.3:52642689-52642708 None:intergenic 50.0%
CGCTTCTTTGAACGGCAGAA+AGG + chr5.3:52640459-52640478 None:intergenic 50.0%
CGTATGCAGCTACTTGCTTC+AGG - chr5.3:52641279-52641298 MS.gene049943:intron 50.0%
GAACGGCAGAAAGGAAACGA+TGG + chr5.3:52640450-52640469 None:intergenic 50.0%
TACATGGTGCAGCTACTGTG+AGG + chr5.3:52641694-52641713 None:intergenic 50.0%
TACCCACATGGATTTCACCC+AGG - chr5.3:52642669-52642688 MS.gene049943:CDS 50.0%
TCCAATGTGTGGTACCCACA+TGG - chr5.3:52642657-52642676 MS.gene049943:CDS 50.0%
TCCATGTGGGTACCACACAT+TGG + chr5.3:52642661-52642680 None:intergenic 50.0%
TCCTTGACCACGATGCTCAA+GGG + chr5.3:52641203-52641222 None:intergenic 50.0%
TGCTTGGTTGGGCCGTAAAA+AGG - chr5.3:52641536-52641555 MS.gene049943:CDS 50.0%
!! GACTTTTCCCTTGAGCATCG+TGG - chr5.3:52641193-52641212 MS.gene049943:intron 50.0%
CACTCTCTCTAACACTCGCC+TGG + chr5.3:52642690-52642709 None:intergenic 55.0%
CGCCTGGGTGAAATCCATGT+GGG + chr5.3:52642674-52642693 None:intergenic 55.0%
GTCCTTGACCACGATGCTCA+AGG + chr5.3:52641204-52641223 None:intergenic 55.0%
TCGCCTGGGTGAAATCCATG+TGG + chr5.3:52642675-52642694 None:intergenic 55.0%
! GAGCATCGTGGTCAAGGACA+TGG - chr5.3:52641205-52641224 MS.gene049943:intron 55.0%
! TCCCTTGAGCATCGTGGTCA+AGG - chr5.3:52641199-52641218 MS.gene049943:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 52640395 52643259 52640395 ID=MS.gene049943
chr5.3 mRNA 52640395 52643259 52640395 ID=MS.gene049943.t1;Parent=MS.gene049943
chr5.3 exon 52642354 52643259 52642354 ID=MS.gene049943.t1.exon1;Parent=MS.gene049943.t1
chr5.3 CDS 52642354 52643259 52642354 ID=cds.MS.gene049943.t1;Parent=MS.gene049943.t1
chr5.3 exon 52641474 52641647 52641474 ID=MS.gene049943.t1.exon2;Parent=MS.gene049943.t1
chr5.3 CDS 52641474 52641647 52641474 ID=cds.MS.gene049943.t1;Parent=MS.gene049943.t1
chr5.3 exon 52640395 52640682 52640395 ID=MS.gene049943.t1.exon3;Parent=MS.gene049943.t1
chr5.3 CDS 52640395 52640682 52640395 ID=cds.MS.gene049943.t1;Parent=MS.gene049943.t1
Gene Sequence

>MS.gene049943

ATGGGAGTGAGCAATGACATATTGAGTTCATTGCCCGAATCATTGCTTTCGGCCATCGTTTCCTTTCTGCCGTTCAAAGAAGCGGTGAGAACTTCTATTCTCTCTAAAGATTGGGTCAATGTATACAAATCAACAAGAAAAATAGAGTTCAATGAACTCTTCTTTGTTAACGTTGATCAATCTCCTGAAATTGTTGATGCTCAAAGAAGGGCATTTTCAGCTTTCGTGAGAAAATGGATTGAAAAACACGGAGAAGATGTCATTGACAAATTCTCTTTAACAATTATGTTGCCTGAATTTTTTAATGAGCTCATTGATCAATGTTTTCGTTTCGCTTTAAGAAATGGAATCAAGGATTTGGAGCTTGATTTCACTTATCAACCTTATGATATTGTTTCAGCAGTAGTTGTTTTGCCTACTTATTTTTATGGTTGTAGTGGGTTTGAATCTTTGAAATTGTATGCATGTAAATTTGATGCTAGTCAATGTGTTAATTTTCATTCACTCAAAAAAATTTCTTTGGGTTACATGGGAATAAAGCTACATGCTATTAAGGCACTTTTGACCAATTGTGAAAAGTTGGAGTGTTTGAGTTTTTTGAAATGCTGGAATTCGGAAGGAGATTTTATTTTGAATGAAGAATATATGGGGTTGAAAAAATTGGTTATCAATAGCTGCGATTTTAAGTTTAATATTTTTAGAGTAAATGCCCCAAATCTAAAGGTTTTCAATTATCATGGATTGATGATACTTTTTGCCGTTGATATTAAATCTCCTGCATTGGATGAGGTTAATCTTGACTTTTCCCTTGAGCATCGTGGTCAAGGACATGGTTCTTATCTTTACAAATTTTTAAAAGAGTTCTCTTCTGCTAAAATTATGACCGTATGCAGCTACTTGCTTCAGGTACCATTTTTTTACCTTTTGTCTTTTTTCTTAAATTTCAAATTTCTTTTTGATAATCAATTTAATTATTATTGCACAATAGATTGCTTTTACTGTATTAATTAACTTTATTCGTTGAAACAATTTATAGCTAGATCTACATATTATTCTTCTCTAATTGTTTTGAAGATCTCTATATTGTATTCATTGGGTTTTTTTTTTTTTTATTGTTTTGCTCTTTTGGGTTCGTGTATTTTGCTTGGTTGGGCCGTAAAAAGGTATATATTTTTCTTAGATTTCAAAGTTTTAAAGGATGGCTGCGTTAAAAATTCTAAAGCTAAATTTTTTTATGAGAAACAACTGAGAAGACCATTAAATTTGTGTGAAATTTTCTTTCATTAGACTTAATGACCTCACAGTAGCTGCACCATGTAGTTGTTATTCATGTCCTTGAGAGAGATATCGATAGAATGCATGAATGCATGCAATATCTTATACTTTCACTAATTTTGTTTTTATTCTAAAAAACATTACTTCTATTATATTAAAGTAATGTTTATAAACTAGGATTAGTCCAATTTTTTTTTTTGAAGGATAGGATTAGTCCAATTGTTAAGCAGTTTTATTTTCCTTTACATGACTTGAATATATATTTAAATAGTTAGCGTAACTTTTAAAAATGAATTTGTTATTATCTTTCTTAATGTTTTGTGTATGATGTGATAAGGTTATTTATACCGAAGGAGGACCAAAACGAATGTATCCTAGCATGAATGTTAAGCATCTGATACTGAAGACCGCTTTGCACCATTTTGAGTTTATGGGAGTATCTTTTATGCTAAGAAGCTGCCCTTACTTAGAGCGTCTCACAATTGAAATTGTTGACGAAAGACACCTGTTTGTAAGTATTACATAAATTTGATTTAATTTAATTAATTTTCTCTCTCTATATGTGTCGATGTAAAATATTTTTATACAGACATGTAGTCATTTTCGTTTTGAGGCAAATATCTAATCATAATTAACCAAATAGAAATTCATTTTGTTCTTGACCAAAATACTTTTAGTTTACTTTATTTTGTTGGGTTGGATTTCACTTGTATTTTTTTTCGTCAATGGGTTTGGTACCACTTATATTTTCTCTCATCAGAAAATAAAATTCATATATATATATATATATATATATATATATATATATATATATATAATTGTAGATTTTAGAAATTAACATAATGAATACCATAGCTTCTTTTTTTGGTTACAAGTATTATTTTTATGGAGAAATGTTAGGAATACTCTCTTTATAGCACTCTCTAACACTCACTTTTCTATTAGGTGAAACGCATGCGAATCTCAACACTTTATGTGAGATCCATTTCCAATGTGTGGTACCCACATGGATTTCACCCAGGCGAGTGTTAGAGAGAGTGTCCATATTACTCATCTTTTTTTGTTTATAAATATCATAGTTTAATTTGACTAAATATATGCATAAAAATATTATAATATTATTCTTTAATTGTGAATTTTTGAAGTAGGTAGGATAAAAATCAAACTATTTTTATCAGTTGATGATCACAATTGATTGAGAGTCTAAACTTACTTGGCACATATAAATTAAATTTTATACTTCATATGCCTTTTGATATAAAATTAAAAACATGGAACTTTTTGTTGTTGTTTATATTGTAGGGTTATGAAGCAATGTTCAATGTTATCCCAGAGATATACTGGGTTGACTCTATAAAAGTTTACAAATGTGTGAAAAACTGTTTGAAGGAAATCGAGATTAAGAGCTTTAAGGGAACTAAAAATGAGTTGAACGTGATTACTTACTTTCTCACTTATGGAAAGGTCTTGAGAAAGTTGAGTATCAATTTCCTTAGAGATGAATGGAATGTCGCTTGTCCCCGTAAAGTAGTAGAGCATTTTCGAATGATTCCAAAAGCTTCAAATAATTTGGAGATATCAATCTATTAG
Protein sequence

>MS.gene049943.t1

MGVSNDILSSLPESLLSAIVSFLPFKEAVRTSILSKDWVNVYKSTRKIEFNELFFVNVDQSPEIVDAQRRAFSAFVRKWIEKHGEDVIDKFSLTIMLPEFFNELIDQCFRFALRNGIKDLELDFTYQPYDIVSAVVVLPTYFYGCSGFESLKLYACKFDASQCVNFHSLKKISLGYMGIKLHAIKALLTNCEKLECLSFLKCWNSEGDFILNEEYMGLKKLVINSCDFKFNIFRVNAPNLKVFNYHGLMILFAVDIKSPALDEVNLDFSLEHRGQGHGSYLYKFLKEFSSAKIMTVCSYLLQVIYTEGGPKRMYPSMNVKHLILKTALHHFEFMGVSFMLRSCPYLERLTIEIVDERHLFGYEAMFNVIPEIYWVDSIKVYKCVKNCLKEIEIKSFKGTKNELNVITYFLTYGKVLRKLSINFLRDEWNVACPRKVVEHFRMIPKASNNLEISIY