AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21278

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21278.t1 MTR_5g080150 55.181 415 142 6 1 407 1 379 3.31e-140 407
MS.gene21278.t1 MTR_2g048140 39.912 456 260 8 6 454 7 455 6.29e-101 309
MS.gene21278.t1 MTR_1g090907 39.696 461 264 8 1 454 1 454 3.29e-99 305
MS.gene21278.t1 MTR_3g448340 36.617 467 272 11 1 452 1 458 1.99e-84 267
MS.gene21278.t1 MTR_1g090903 32.969 458 248 11 4 454 5 410 5.68e-63 209
MS.gene21278.t1 MTR_7g029260 60.317 126 50 0 302 427 34 159 2.51e-44 155
MS.gene21278.t1 MTR_3g049530 33.240 358 179 11 134 455 84 417 1.52e-40 150
MS.gene21278.t1 MTR_5g066890 70.930 86 23 1 317 402 1 84 9.52e-33 119
MS.gene21278.t1 MTR_5g066900 73.737 99 11 1 1 99 1 84 2.10e-31 115
MS.gene21278.t1 MTR_5g080160 52.525 99 41 1 362 454 61 159 3.95e-25 101
MS.gene21278.t1 MTR_5g080160 66.667 57 18 1 1 56 1 57 2.80e-13 67.8
MS.gene21278.t1 MTR_2g047858 25.000 428 269 13 6 421 115 502 1.68e-21 97.4
MS.gene21278.t1 MTR_5g080110 59.701 67 27 0 1 67 1 67 2.24e-17 76.6
MS.gene21278.t1 MTR_1g021825 23.570 437 256 16 6 421 88 467 8.18e-17 83.2
MS.gene21278.t1 MTR_1g021825 23.570 437 256 16 6 421 17 396 8.51e-17 82.8
MS.gene21278.t1 MTR_8g468470 26.906 446 274 20 4 422 12 432 1.23e-16 82.4
MS.gene21278.t1 MTR_5g092960 23.840 474 307 16 6 455 19 462 1.39e-16 82.0
MS.gene21278.t1 MTR_1g016340 23.990 421 283 12 6 419 15 405 5.70e-16 80.1
MS.gene21278.t1 MTR_1g016340 23.990 421 283 12 6 419 20 410 8.06e-16 79.7
MS.gene21278.t1 MTR_2g072720 25.000 468 274 19 5 441 38 459 3.77e-15 77.8
MS.gene21278.t1 MTR_8g467800 24.490 441 275 15 5 422 19 424 4.37e-15 77.4
MS.gene21278.t1 MTR_5g084070 24.123 456 293 11 6 452 13 424 7.69e-15 76.6
MS.gene21278.t1 MTR_5g080090 49.333 75 28 2 331 404 39 104 1.14e-14 70.5
MS.gene21278.t1 MTR_1g013840 23.529 442 272 16 1 423 51 445 1.30e-13 73.2
MS.gene21278.t1 MTR_8g468810 23.779 471 300 15 6 452 25 460 2.84e-13 72.0
MS.gene21278.t1 MTR_4g045900 22.363 474 291 17 6 421 26 480 3.14e-13 72.0
MS.gene21278.t1 MTR_8g014160 31.188 202 122 9 6 197 41 235 5.97e-13 70.9
MS.gene21278.t1 MTR_2g091145 23.419 427 273 15 6 419 21 406 1.83e-12 69.3
MS.gene21278.t1 MTR_0049s0110 28.205 195 127 6 6 199 8 190 1.94e-12 68.6
MS.gene21278.t1 MTR_1g011400 21.918 438 270 12 6 421 7 394 1.95e-12 69.3
MS.gene21278.t1 MTR_0049s0110 28.205 195 127 6 6 199 8 190 1.96e-12 67.8
MS.gene21278.t1 MTR_0049s0110 28.205 195 127 6 6 199 8 190 2.08e-12 68.6
MS.gene21278.t1 MTR_4g080590 22.770 426 290 13 6 421 23 419 2.30e-12 68.9
MS.gene21278.t1 MTR_0049s0110 22.658 459 292 15 6 452 8 415 2.38e-12 68.9
MS.gene21278.t1 MTR_4g103670 23.007 439 255 18 6 422 25 402 4.18e-12 68.2
MS.gene21278.t1 MTR_7g076760 23.529 442 264 17 6 419 21 416 6.57e-12 67.8
MS.gene21278.t1 MTR_2g072730 23.937 447 272 17 6 423 16 423 7.50e-12 67.4
MS.gene21278.t1 MTR_5g040850 22.951 427 279 12 3 421 4 388 9.09e-12 67.0
MS.gene21278.t1 MTR_8g062010 25.751 466 274 23 4 447 7 422 9.73e-12 67.0
MS.gene21278.t1 MTR_5g080120 55.556 54 24 0 317 370 1 54 1.13e-11 60.1
MS.gene21278.t1 MTR_1g071060 38.636 88 53 1 38 125 7 93 2.43e-11 60.5
MS.gene21278.t1 MTR_2g047848 23.148 432 271 17 5 419 6 393 2.47e-11 65.9
MS.gene21278.t1 MTR_8g062310 25.486 463 265 19 2 447 21 420 4.93e-11 64.7
MS.gene21278.t1 MTR_4g088845 22.642 424 287 13 6 419 19 411 8.77e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21278.t1 AT1G56400 33.100 429 261 9 2 414 9 427 1.24e-65 218
MS.gene21278.t1 AT3G62230 30.258 466 305 8 2 455 4 461 8.61e-60 202
MS.gene21278.t1 AT5G54820 30.841 428 266 8 2 410 3 419 2.91e-58 199
MS.gene21278.t1 AT1G56400 31.469 429 227 10 2 414 9 386 2.41e-53 185
MS.gene21278.t1 AT3G29830 29.060 468 301 12 6 455 8 462 3.61e-53 185
MS.gene21278.t1 AT3G29830 29.060 468 300 13 6 455 8 461 1.32e-52 184
MS.gene21278.t1 AT2G17305 31.548 168 105 3 294 455 106 269 1.52e-23 100
MS.gene21278.t1 AT1G61330 25.114 438 291 15 7 431 11 424 3.62e-23 102
MS.gene21278.t1 AT3G58880 24.528 424 279 13 6 409 3 405 9.55e-18 85.9
MS.gene21278.t1 AT4G26340 24.330 448 299 15 6 449 2 413 3.23e-17 84.0
MS.gene21278.t1 AT3G58860 24.292 424 291 12 6 421 7 408 9.33e-17 82.8
MS.gene21278.t1 AT4G03220 24.089 494 312 19 6 455 24 498 1.66e-16 82.0
MS.gene21278.t1 AT3G28410 21.909 461 318 11 6 454 28 458 6.57e-16 80.1
MS.gene21278.t1 AT5G53635 24.658 438 274 15 7 422 1 404 7.50e-16 79.7
MS.gene21278.t1 AT1G61320 22.426 437 299 14 10 431 25 436 9.98e-16 79.7
MS.gene21278.t1 AT3G52680 24.729 461 300 17 3 452 18 442 1.27e-15 79.3
MS.gene21278.t1 AT3G42770 22.826 460 306 14 8 454 1 424 4.69e-15 77.8
MS.gene21278.t1 AT1G16930 21.519 474 307 11 4 452 13 446 5.86e-15 77.0
MS.gene21278.t1 AT1G16930 21.519 474 307 11 4 452 13 446 5.86e-15 77.0
MS.gene21278.t1 AT1G16930 21.519 474 307 11 4 452 13 446 5.86e-15 77.0
MS.gene21278.t1 AT1G32375 25.754 431 265 17 6 422 2 391 1.08e-14 76.3
MS.gene21278.t1 AT1G32375 25.754 431 265 17 6 422 2 391 1.08e-14 76.3
MS.gene21278.t1 AT5G44980 23.465 456 263 14 3 423 1 405 1.40e-14 75.9
MS.gene21278.t1 AT5G44980 23.465 456 263 14 3 423 1 405 1.40e-14 75.9
MS.gene21278.t1 AT4G26350 24.374 439 259 16 6 418 2 393 1.42e-14 75.9
MS.gene21278.t1 AT5G22730 21.749 446 284 11 6 423 28 436 1.74e-14 75.9
MS.gene21278.t1 AT5G22730 21.749 446 284 11 6 423 28 436 1.74e-14 75.9
MS.gene21278.t1 AT3G58900 23.690 439 295 11 6 421 2 423 7.25e-14 73.9
MS.gene21278.t1 AT5G53840 24.520 469 297 16 2 455 14 440 1.27e-13 73.2
MS.gene21278.t1 AT5G22610 24.301 465 291 17 6 455 18 436 1.28e-13 73.2
MS.gene21278.t1 AT1G66310 22.462 463 305 15 6 454 19 441 2.31e-13 72.0
MS.gene21278.t1 AT3G52680 25.967 362 230 13 3 358 18 347 2.89e-13 71.2
MS.gene21278.t1 AT3G58940 24.945 453 278 16 6 434 2 416 4.48e-13 71.6
MS.gene21278.t1 AT3G58940 24.945 453 278 16 6 434 2 416 4.48e-13 71.6
MS.gene21278.t1 AT1G78760 21.810 431 287 13 6 421 16 411 7.09e-13 70.9
MS.gene21278.t1 AT4G00160 29.482 251 145 8 3 241 13 243 9.55e-13 70.5
MS.gene21278.t1 AT5G02930 24.390 369 224 11 6 351 28 364 1.78e-12 69.7
MS.gene21278.t1 AT3G59240 21.219 443 302 13 6 423 8 428 2.01e-12 69.3
MS.gene21278.t1 AT5G02910 25.876 371 234 12 6 362 11 354 2.16e-12 69.3
MS.gene21278.t1 AT3G59000 24.890 454 272 16 6 421 2 424 2.24e-12 69.3
MS.gene21278.t1 AT5G02910 25.698 358 230 11 6 354 11 341 2.65e-12 68.6
MS.gene21278.t1 AT5G02910 25.698 358 230 11 6 354 11 341 3.12e-12 68.2
MS.gene21278.t1 AT1G13570 28.500 200 129 6 1 199 1 187 4.59e-12 67.0
MS.gene21278.t1 AT1G80470 22.996 474 290 20 5 454 14 436 7.47e-12 67.8
MS.gene21278.t1 AT5G22670 22.697 445 278 15 4 423 9 412 1.04e-11 67.0
MS.gene21278.t1 AT1G13570 28.631 241 152 10 1 240 1 222 1.57e-11 66.2
MS.gene21278.t1 AT1G13570 28.631 241 152 10 1 240 1 222 1.57e-11 66.2
MS.gene21278.t1 AT4G14103 27.670 206 124 6 1 197 3 192 2.80e-11 65.5
MS.gene21278.t1 AT4G14103 27.670 206 124 6 1 197 3 192 2.80e-11 65.5
MS.gene21278.t1 AT3G44060 22.198 464 306 18 8 454 1 426 3.34e-11 65.5
MS.gene21278.t1 AT4G14103 28.079 203 121 6 4 197 6 192 3.40e-11 65.5
MS.gene21278.t1 AT1G69630 23.112 437 280 16 6 421 18 419 3.93e-11 65.5
MS.gene21278.t1 AT2G42730 22.128 470 325 18 4 454 3 450 8.55e-11 64.3
MS.gene21278.t1 AT3G58920 22.811 491 312 17 6 454 2 467 8.58e-11 64.3

Find 59 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAAAGCTTCAAATAATT+TGG 0.116785 5.1:-51859058 MS.gene21278:CDS
CTTTGAGCATCAACAATTTC+AGG 0.151883 5.1:+51861688 None:intergenic
AATCGAGATTAAGAGCTTTA+AGG 0.179493 5.1:-51859217 MS.gene21278:CDS
TCCAAATTATTTGAAGCTTT+TGG 0.187191 5.1:+51859057 None:intergenic
ATTACTTACTTTCTCACTTA+TGG 0.189242 5.1:-51859171 MS.gene21278:CDS
AATTGAAAACCTTTAGATTT+GGG 0.237542 5.1:+51861161 None:intergenic
TTTCAGCTTTCGTGAGAAAA+TGG 0.248575 5.1:-51861657 MS.gene21278:CDS
TTTGGAGATATCAATCTATT+AGG 0.275918 5.1:-51859040 None:intergenic
TAATTGAAAACCTTTAGATT+TGG 0.278932 5.1:+51861160 None:intergenic
ATGTTATCCCAGAGATATAC+TGG 0.291076 5.1:-51859287 MS.gene21278:CDS
AAGAAATGGAATCAAGGATT+TGG 0.294548 5.1:-51861533 MS.gene21278:CDS
AATAAAGCTACATGCTATTA+AGG 0.302871 5.1:-51861338 MS.gene21278:CDS
GAAAATGCTCTACTGCTTTA+CGG 0.316225 5.1:+51859088 None:intergenic
ATCGAGATTAAGAGCTTTAA+GGG 0.324791 5.1:-51859216 MS.gene21278:CDS
AAAATGCTCTACTGCTTTAC+GGG 0.336374 5.1:+51859089 None:intergenic
CTTCTATTCTCTCTAAAGAT+TGG 0.337117 5.1:-51861780 MS.gene21278:CDS
TTTCGTTTCGCTTTAAGAAA+TGG 0.339603 5.1:-51861547 MS.gene21278:CDS
ATGCTAGGATACATTCGTTT+TGG 0.347569 5.1:+51860280 None:intergenic
CGACATTCCATTCATCTCTA+AGG 0.355244 5.1:+51859118 None:intergenic
ATTGCCCGAATCATTGCTTT+CGG 0.390421 5.1:-51861842 MS.gene21278:CDS
GAGTCAACCCAGTATATCTC+TGG 0.398444 5.1:+51859279 None:intergenic
TTCTATTCTCTCTAAAGATT+GGG 0.400740 5.1:-51861779 MS.gene21278:CDS
TTTGACCAATTGTGAAAAGT+TGG 0.408885 5.1:-51861311 MS.gene21278:CDS
CGTATGCAGCTACTTGCTTC+AGG 0.420928 5.1:-51860987 MS.gene21278:intron
CGCTTTAAGAAATGGAATCA+AGG 0.433111 5.1:-51861539 MS.gene21278:CDS
TCAATTTCCTTAGAGATGAA+TGG 0.436949 5.1:-51859125 MS.gene21278:CDS
GATGGCCGAAAGCAATGATT+CGG 0.465360 5.1:+51861837 None:intergenic
ATTGTGAGACGCTCTAAGTA+AGG 0.466101 5.1:+51860178 None:intergenic
AGTAAATGCCCCAAATCTAA+AGG 0.472229 5.1:-51861170 MS.gene21278:CDS
TCCCTTGAGCATCGTGGTCA+AGG 0.474795 5.1:-51861067 MS.gene21278:CDS
TGATATTAAATCTCCTGCAT+TGG 0.479200 5.1:-51861110 MS.gene21278:CDS
ATGGCCGAAAGCAATGATTC+GGG 0.488444 5.1:+51861838 None:intergenic
ACTGCTGAAACAATATCATA+AGG 0.492198 5.1:+51861490 None:intergenic
CGCTTCTTTGAACGGCAGAA+AGG 0.500724 5.1:+51861810 None:intergenic
AAAATTTCTTTGGGTTACAT+GGG 0.516495 5.1:-51861361 MS.gene21278:CDS
AAATTGTTGATGCTCAAAGA+AGG 0.517077 5.1:-51861684 MS.gene21278:CDS
TAAATCTCCTGCATTGGATG+AGG 0.519266 5.1:-51861104 MS.gene21278:CDS
CTTTCTGCCGTTCAAAGAAG+CGG 0.527237 5.1:-51861809 MS.gene21278:CDS
TTGAAATGCTGGAATTCGGA+AGG 0.539765 5.1:-51861274 MS.gene21278:CDS
TTACTTTCTCACTTATGGAA+AGG 0.540191 5.1:-51859166 MS.gene21278:CDS
ATTGAAAACCTTTAGATTTG+GGG 0.540504 5.1:+51861162 None:intergenic
TGCAGGAGATTTAATATCAA+CGG 0.548324 5.1:+51861114 None:intergenic
GTCCTTGACCACGATGCTCA+AGG 0.552550 5.1:+51861065 None:intergenic
TATGTCATTGCTCATTCCCA+TGG 0.552959 5.1:+51861873 None:intergenic
GTTCTCACCGCTTCTTTGAA+CGG 0.559469 5.1:+51861802 None:intergenic
GAGCATCGTGGTCAAGGACA+TGG 0.568121 5.1:-51861061 MS.gene21278:CDS
AGATTAACCTCATCCAATGC+AGG 0.570665 5.1:+51861097 None:intergenic
TCCTTGACCACGATGCTCAA+GGG 0.583759 5.1:+51861066 None:intergenic
AGATGCTTAACATTCATGCT+AGG 0.592384 5.1:+51860265 None:intergenic
AGTCAACCCAGTATATCTCT+GGG 0.594219 5.1:+51859280 None:intergenic
AAGGTTATTTATACCGAAGG+AGG 0.595848 5.1:-51860304 MS.gene21278:CDS
TTGTGAGACGCTCTAAGTAA+GGG 0.602951 5.1:+51860179 None:intergenic
CTGAAGCAAGTAGCTGCATA+CGG 0.604279 5.1:+51860988 None:intergenic
GATAAGGTTATTTATACCGA+AGG 0.612007 5.1:-51860307 MS.gene21278:intron
AATTGTTGATGCTCAAAGAA+GGG 0.622812 5.1:-51861683 MS.gene21278:CDS
TGTTATCCCAGAGATATACT+GGG 0.674333 5.1:-51859286 MS.gene21278:CDS
GAACGGCAGAAAGGAAACGA+TGG 0.683061 5.1:+51861819 None:intergenic
AAATGCTCTACTGCTTTACG+GGG 0.715301 5.1:+51859090 None:intergenic
AAACTCAAAATGGTGCAAAG+CGG 0.726269 5.1:+51860231 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATATAAAATTAAAAACA+TGG - chr5.1:51861556-51861575 MS.gene21278:CDS 10.0%
!!! AAAGTAAACTAAAATTATTT+TGG + chr5.1:51860970-51860989 None:intergenic 10.0%
!!! GTTTTTAATTTTATATCAAA+AGG + chr5.1:51861555-51861574 None:intergenic 10.0%
!! AAAATTTCACACAAATTTAA+TGG + chr5.1:51860295-51860314 None:intergenic 15.0%
!! AATTTAAGAAAAAAGACAAA+AGG + chr5.1:51859964-51859983 None:intergenic 15.0%
!! CTTCTATTATATTAAAGTAA+TGG - chr5.1:51860448-51860467 MS.gene21278:intron 15.0%
!!! ATTTTGAATGAAGAATATAT+GGG - chr5.1:51859668-51859687 MS.gene21278:intron 15.0%
!!! TATTTTGAATGAAGAATATA+TGG - chr5.1:51859667-51859686 MS.gene21278:intron 15.0%
!!! TTAGTTTACTTTATTTTGTT+TGG - chr5.1:51860978-51860997 MS.gene21278:intron 15.0%
!! AAAGACAAAAGGTAAAAAAA+TGG + chr5.1:51859953-51859972 None:intergenic 20.0%
!! AAATAAAACTGCTTAACAAT+TGG + chr5.1:51860492-51860511 None:intergenic 20.0%
!! AATTGAAAACCTTTAGATTT+GGG + chr5.1:51859754-51859773 None:intergenic 20.0%
!! ATCTCTATATTGTATTCATT+GGG - chr5.1:51860115-51860134 MS.gene21278:intron 20.0%
!! GAACAAAATGAATTTCTATT+TGG + chr5.1:51860943-51860962 None:intergenic 20.0%
!! TAAAGTAATGGTTATAAACT+AGG - chr5.1:51860460-51860479 MS.gene21278:intron 20.0%
!! TAATTGAAAACCTTTAGATT+TGG + chr5.1:51859755-51859774 None:intergenic 20.0%
!! TATATATTCAAGTCATGTAA+AGG + chr5.1:51860516-51860535 None:intergenic 20.0%
!! TCACTCAAAAAAATTTCTTT+GGG - chr5.1:51859542-51859561 MS.gene21278:intron 20.0%
!! TGTGTATTATGCATTTAATT+TGG - chr5.1:51860839-51860858 MS.gene21278:intron 20.0%
!! TTCACTCAAAAAAATTTCTT+TGG - chr5.1:51859541-51859560 MS.gene21278:intron 20.0%
!! TTTATGTAATACTTACAAAC+AGG + chr5.1:51860781-51860800 None:intergenic 20.0%
!!! GGTTACAAGTATTATTTTTA+TGG - chr5.1:51861170-51861189 MS.gene21278:CDS 20.0%
!!! TAATTGTGAATTTTTGAAGT+AGG - chr5.1:51861430-51861449 MS.gene21278:CDS 20.0%
!!! TATTTTTATGGAGAAATGTT+AGG - chr5.1:51861182-51861201 MS.gene21278:CDS 20.0%
!!! TTGTATTTTTTTTCGTCAAT+GGG - chr5.1:51861012-51861031 MS.gene21278:CDS 20.0%
!!! TTGTTGTTGTTTATATTGTA+GGG - chr5.1:51861585-51861604 MS.gene21278:CDS 20.0%
!!! TTTACTTTATTTTGTTTGGT+TGG - chr5.1:51860982-51861001 MS.gene21278:intron 20.0%
!!! TTTGTTGTTGTTTATATTGT+AGG - chr5.1:51861584-51861603 MS.gene21278:CDS 20.0%
!!! TTTTGAATGAAGAATATATG+GGG - chr5.1:51859669-51859688 MS.gene21278:intron 20.0%
! AATAAAGCTACATGCTATTA+AGG - chr5.1:51859574-51859593 MS.gene21278:intron 25.0%
! ACAAAAAAAGATGAGTGATA+TGG + chr5.1:51861348-51861367 None:intergenic 25.0%
! ATATATGGGGTTGAAAAAAT+TGG - chr5.1:51859682-51859701 MS.gene21278:intron 25.0%
! ATTACTTACTTTCTCACTTA+TGG - chr5.1:51861741-51861760 MS.gene21278:CDS 25.0%
! ATTGAAAACCTTTAGATTTG+GGG + chr5.1:51859753-51859772 None:intergenic 25.0%
! CTACAACCATAAAAATAAGT+AGG + chr5.1:51859458-51859477 None:intergenic 25.0%
! GATCTCTATATTGTATTCAT+TGG - chr5.1:51860114-51860133 MS.gene21278:intron 25.0%
! TCCAAAAGCTTCAAATAATT+TGG - chr5.1:51861854-51861873 MS.gene21278:CDS 25.0%
!! AAAAATTTCTTTGGGTTACA+TGG - chr5.1:51859550-51859569 MS.gene21278:intron 25.0%
!! AAAATTTCTTTGGGTTACAT+GGG - chr5.1:51859551-51859570 MS.gene21278:intron 25.0%
!! CTAAAGGTTTTCAATTATCA+TGG - chr5.1:51859758-51859777 MS.gene21278:intron 25.0%
!! TTCTATTCTCTCTAAAGATT+GGG - chr5.1:51859133-51859152 MS.gene21278:CDS 25.0%
!!! AATACCATAGCTTCTTTTTT+TGG - chr5.1:51861149-51861168 MS.gene21278:CDS 25.0%
!!! ACTTATTTTTATGGTTGTAG+TGG - chr5.1:51859458-51859477 MS.gene21278:intron 25.0%
!!! AGAAAATGGATTGAAAAACA+CGG - chr5.1:51859269-51859288 MS.gene21278:CDS 25.0%
!!! CTTAGATTTCAAAGTTTTAG+AGG - chr5.1:51860213-51860232 MS.gene21278:CDS 25.0%
!!! CTTATTTTTATGGTTGTAGT+GGG - chr5.1:51859459-51859478 MS.gene21278:intron 25.0%
!!! CTTGTATTTTTTTTCGTCAA+TGG - chr5.1:51861011-51861030 MS.gene21278:CDS 25.0%
!!! GTTTTGCCTACTTATTTTTA+TGG - chr5.1:51859449-51859468 MS.gene21278:intron 25.0%
!!! TCCAAATTATTTGAAGCTTT+TGG + chr5.1:51861858-51861877 None:intergenic 25.0%
!!! TTTTATTGTTTTGCTCTCTT+GGG - chr5.1:51860145-51860164 MS.gene21278:intron 25.0%
!!! TTTTTATTGTTTTGCTCTCT+TGG - chr5.1:51860144-51860163 MS.gene21278:intron 25.0%
!!! TTTTTTGAAATGCTGGAATT+CGG - chr5.1:51859634-51859653 MS.gene21278:intron 25.0%
AAATTGTTGATGCTCAAAGA+AGG - chr5.1:51859228-51859247 MS.gene21278:CDS 30.0%
AAGAAATGGAATCAAGGATT+TGG - chr5.1:51859379-51859398 MS.gene21278:intron 30.0%
AATCGAGATTAAGAGCTTTA+AGG - chr5.1:51861695-51861714 MS.gene21278:CDS 30.0%
AATTGTTGATGCTCAAAGAA+GGG - chr5.1:51859229-51859248 MS.gene21278:CDS 30.0%
ACTGCTGAAACAATATCATA+AGG + chr5.1:51859425-51859444 None:intergenic 30.0%
ATACTCCCAAAAACTCAAAA+TGG + chr5.1:51860694-51860713 None:intergenic 30.0%
ATGAGCTCACTAAAAAATTC+AGG + chr5.1:51859335-51859354 None:intergenic 30.0%
ATGTGTGAAAAACTGTTTGA+AGG - chr5.1:51861671-51861690 MS.gene21278:CDS 30.0%
ATTGAGAGTCTAAACTTACT+TGG - chr5.1:51861498-51861517 MS.gene21278:CDS 30.0%
CTGTTGAGAGAAAATATAAG+TGG + chr5.1:51861045-51861064 None:intergenic 30.0%
GATAAGGTTATTTATACCGA+AGG - chr5.1:51860605-51860624 MS.gene21278:intron 30.0%
GTAACCAAAAAAAGAAGCTA+TGG + chr5.1:51861156-51861175 None:intergenic 30.0%
TCAATTTCCTTAGAGATGAA+TGG - chr5.1:51861787-51861806 MS.gene21278:CDS 30.0%
TGATATTAAATCTCCTGCAT+TGG - chr5.1:51859802-51859821 MS.gene21278:intron 30.0%
TGCAGGAGATTTAATATCAA+CGG + chr5.1:51859801-51859820 None:intergenic 30.0%
TTACTTTCTCACTTATGGAA+AGG - chr5.1:51861746-51861765 MS.gene21278:CDS 30.0%
TTTGACCAATTGTGAAAAGT+TGG - chr5.1:51859601-51859620 MS.gene21278:intron 30.0%
! ATCGAGATTAAGAGCTTTAA+GGG - chr5.1:51861696-51861715 MS.gene21278:CDS 30.0%
! CTTCTATTCTCTCTAAAGAT+TGG - chr5.1:51859132-51859151 MS.gene21278:CDS 30.0%
! GTTTTGTGTATGATGTGATA+AGG - chr5.1:51860589-51860608 MS.gene21278:intron 30.0%
!! ATGTAGTCATTTTCGTTTTG+AGG - chr5.1:51860896-51860915 MS.gene21278:intron 30.0%
!! CTAACACTCACTTTTCTATT+AGG - chr5.1:51861227-51861246 MS.gene21278:CDS 30.0%
!! TTTCGTTTCGCTTTAAGAAA+TGG - chr5.1:51859365-51859384 MS.gene21278:intron 30.0%
!!! GATTTCAAAGTTTTAGAGGA+TGG - chr5.1:51860217-51860236 MS.gene21278:CDS 30.0%
!!! GTTTGAGTTTTTTGAAATGC+TGG - chr5.1:51859627-51859646 MS.gene21278:intron 30.0%
!!! TGTGAATTTTTGAAGTAGGT+AGG - chr5.1:51861434-51861453 MS.gene21278:CDS 30.0%
!!! TTGCACCATTTTGAGTTTTT+GGG - chr5.1:51860686-51860705 MS.gene21278:intron 30.0%
!!! TTTGCACCATTTTGAGTTTT+TGG - chr5.1:51860685-51860704 MS.gene21278:intron 30.0%
!!! TTTTTTTTCGTCAATGGGTT+TGG - chr5.1:51861017-51861036 MS.gene21278:CDS 30.0%
AAAATGCTCTACTGCTTTAC+GGG + chr5.1:51861826-51861845 None:intergenic 35.0%
AAACTCAAAATGGTGCAAAG+CGG + chr5.1:51860684-51860703 None:intergenic 35.0%
AAGGTTATTTATACCGAAGG+AGG - chr5.1:51860608-51860627 MS.gene21278:intron 35.0%
AGATGCTTAACATTCATGCT+AGG + chr5.1:51860650-51860669 None:intergenic 35.0%
AGTAAATGCCCCAAATCTAA+AGG - chr5.1:51859742-51859761 MS.gene21278:intron 35.0%
ATGTTATCCCAGAGATATAC+TGG - chr5.1:51861625-51861644 MS.gene21278:CDS 35.0%
CTTTGAGCATCAACAATTTC+AGG + chr5.1:51859227-51859246 None:intergenic 35.0%
GAAAATGCTCTACTGCTTTA+CGG + chr5.1:51861827-51861846 None:intergenic 35.0%
GAAATGGATCTCACATAAAG+TGG + chr5.1:51861273-51861292 None:intergenic 35.0%
GGACATGAATAACAACTACA+TGG + chr5.1:51860353-51860372 None:intergenic 35.0%
TGTTATCCCAGAGATATACT+GGG - chr5.1:51861626-51861645 MS.gene21278:CDS 35.0%
TTCTATCGATATCTCTCTCA+AGG + chr5.1:51860374-51860393 None:intergenic 35.0%
TTTCAGCTTTCGTGAGAAAA+TGG - chr5.1:51859255-51859274 MS.gene21278:CDS 35.0%
! ACACTCCAACTTTTCACAAT+TGG + chr5.1:51859609-51859628 None:intergenic 35.0%
! ATGCTAGGATACATTCGTTT+TGG + chr5.1:51860635-51860654 None:intergenic 35.0%
! TTCGTGTATTTTGCTTGGTT+GGG - chr5.1:51860168-51860187 MS.gene21278:CDS 35.0%
!! CGCTTTAAGAAATGGAATCA+AGG - chr5.1:51859373-51859392 MS.gene21278:intron 35.0%
AAATGCTCTACTGCTTTACG+GGG + chr5.1:51861825-51861844 None:intergenic 40.0%
AGATTAACCTCATCCAATGC+AGG + chr5.1:51859818-51859837 None:intergenic 40.0%
AGTCAACCCAGTATATCTCT+GGG + chr5.1:51861635-51861654 None:intergenic 40.0%
ATTGCCCGAATCATTGCTTT+CGG - chr5.1:51859070-51859089 MS.gene21278:CDS 40.0%
CGACATTCCATTCATCTCTA+AGG + chr5.1:51861797-51861816 None:intergenic 40.0%
TAAATCTCCTGCATTGGATG+AGG - chr5.1:51859808-51859827 MS.gene21278:intron 40.0%
TGAGATCCATTTCCAATGTG+TGG - chr5.1:51861280-51861299 MS.gene21278:CDS 40.0%
TTGAAATGCTGGAATTCGGA+AGG - chr5.1:51859638-51859657 MS.gene21278:intron 40.0%
! ATTGTGAGACGCTCTAAGTA+AGG + chr5.1:51860737-51860756 None:intergenic 40.0%
! GTTCGTGTATTTTGCTTGGT+TGG - chr5.1:51860167-51860186 MS.gene21278:CDS 40.0%
! TTGGGTTCGTGTATTTTGCT+TGG - chr5.1:51860163-51860182 MS.gene21278:CDS 40.0%
! TTGTGAGACGCTCTAAGTAA+GGG + chr5.1:51860736-51860755 None:intergenic 40.0%
ACTCTCTCTAACACTCGCTT+GGG + chr5.1:51861323-51861342 None:intergenic 45.0%
ATGGCCGAAAGCAATGATTC+GGG + chr5.1:51859077-51859096 None:intergenic 45.0%
CTGAAGCAAGTAGCTGCATA+CGG + chr5.1:51859927-51859946 None:intergenic 45.0%
CTTTCTGCCGTTCAAAGAAG+CGG - chr5.1:51859103-51859122 MS.gene21278:CDS 45.0%
GAGTCAACCCAGTATATCTC+TGG + chr5.1:51861636-51861655 None:intergenic 45.0%
GATGGCCGAAAGCAATGATT+CGG + chr5.1:51859078-51859097 None:intergenic 45.0%
GTTCTCACCGCTTCTTTGAA+CGG + chr5.1:51859113-51859132 None:intergenic 45.0%
TGCTTGGTTGGGCAGTAAAA+AGG - chr5.1:51860179-51860198 MS.gene21278:CDS 45.0%
TGGGTACCACACATTGGAAA+TGG + chr5.1:51861289-51861308 None:intergenic 45.0%
!! CATTCGTTTTGGTCCTCCTT+CGG + chr5.1:51860624-51860643 None:intergenic 45.0%
CACTCTCTCTAACACTCGCT+TGG + chr5.1:51861324-51861343 None:intergenic 50.0%
CGCTTCTTTGAACGGCAGAA+AGG + chr5.1:51859105-51859124 None:intergenic 50.0%
CGCTTGGGTGAAATCCATGT+GGG + chr5.1:51861308-51861327 None:intergenic 50.0%
CGTATGCAGCTACTTGCTTC+AGG - chr5.1:51859925-51859944 MS.gene21278:intron 50.0%
GAACGGCAGAAAGGAAACGA+TGG + chr5.1:51859096-51859115 None:intergenic 50.0%
TACATGGTGCAGCTACTGTG+AGG + chr5.1:51860337-51860356 None:intergenic 50.0%
TCCAATGTGTGGTACCCACA+TGG - chr5.1:51861291-51861310 MS.gene21278:CDS 50.0%
TCCATGTGGGTACCACACAT+TGG + chr5.1:51861295-51861314 None:intergenic 50.0%
TCCTTGACCACGATGCTCAA+GGG + chr5.1:51859849-51859868 None:intergenic 50.0%
TCGCTTGGGTGAAATCCATG+TGG + chr5.1:51861309-51861328 None:intergenic 50.0%
!! GACTTTTCCCTTGAGCATCG+TGG - chr5.1:51859839-51859858 MS.gene21278:intron 50.0%
GTCCTTGACCACGATGCTCA+AGG + chr5.1:51859850-51859869 None:intergenic 55.0%
! GAGCATCGTGGTCAAGGACA+TGG - chr5.1:51859851-51859870 MS.gene21278:intron 55.0%
! TCCCTTGAGCATCGTGGTCA+AGG - chr5.1:51859845-51859864 MS.gene21278:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 51859041 51861893 51859041 ID=MS.gene21278
chr5.1 mRNA 51859041 51861893 51859041 ID=MS.gene21278.t1;Parent=MS.gene21278
chr5.1 exon 51860988 51861893 51860988 ID=MS.gene21278.t1.exon1;Parent=MS.gene21278.t1
chr5.1 CDS 51860988 51861893 51860988 ID=cds.MS.gene21278.t1;Parent=MS.gene21278.t1
chr5.1 exon 51860150 51860323 51860150 ID=MS.gene21278.t1.exon2;Parent=MS.gene21278.t1
chr5.1 CDS 51860150 51860323 51860150 ID=cds.MS.gene21278.t1;Parent=MS.gene21278.t1
chr5.1 exon 51859041 51859328 51859041 ID=MS.gene21278.t1.exon3;Parent=MS.gene21278.t1
chr5.1 CDS 51859041 51859328 51859041 ID=cds.MS.gene21278.t1;Parent=MS.gene21278.t1
Gene Sequence

>MS.gene21278

ATGGGAATGAGCAATGACATATTGAGTTCATTGCCCGAATCATTGCTTTCGGCCATCGTTTCCTTTCTGCCGTTCAAAGAAGCGGTGAGAACTTCTATTCTCTCTAAAGATTGGGTCAGTGTATACAAATCAACAAGAAAAATAGAGTTCAATGAACTCTTCTTTGTTAACGTTGATCAATCTCCTGAAATTGTTGATGCTCAAAGAAGGGCATTTTCAGCTTTCGTGAGAAAATGGATTGAAAAACACGGAGAAGATGTCATTGACAAATTCTCTTTAACAATTATGTTGCCTGAATTTTTTAGTGAGCTCATTGATCAATGTTTTCGTTTCGCTTTAAGAAATGGAATCAAGGATTTGGAGCTTGATTTCACTTATCAACCTTATGATATTGTTTCAGCAGTAGTTGTTTTGCCTACTTATTTTTATGGTTGTAGTGGGTTTGAATCTTTGAAATTGTATGCATGTAAATTTGATGCTAGTCAATGTGTTAATTTTCATTCACTCAAAAAAATTTCTTTGGGTTACATGGGAATAAAGCTACATGCTATTAAGGCACTTTTGACCAATTGTGAAAAGTTGGAGTGTTTGAGTTTTTTGAAATGCTGGAATTCGGAAGGAGATTTTATTTTGAATGAAGAATATATGGGGTTGAAAAAATTGGTTATCAATAGCTGCGATTTTAAGTTTAATATTTTTAGAGTAAATGCCCCAAATCTAAAGGTTTTCAATTATCATGGATTGATGATACTTTTTGCCGTTGATATTAAATCTCCTGCATTGGATGAGGTTAATCTTGACTTTTCCCTTGAGCATCGTGGTCAAGGACATGGTTCTTATCTTTACAAATTTTTAAAAGAGTTCTCTTCTGCTAAAATTATGACCGTATGCAGCTACTTGCTTCAGGTACCATTTTTTTACCTTTTGTCTTTTTTCTTAAATTTCAAATTTCTTTTTGATAATCAATTTAATTATTATTGCACAATAGATTGCTTTTACTGTATTAATTAACTTTATTCGTTGAAACAATTTATAGCTAGATCTACATATTATTCTTCTCTAATTGTTTTGAAGATCTCTATATTGTATTCATTGGGTTTTTTTTTTTATTGTTTTGCTCTCTTGGGTTCGTGTATTTTGCTTGGTTGGGCAGTAAAAAGGTATATATTTTTCTTAGATTTCAAAGTTTTAGAGGATGGCTGCGTTAAAAATTCTAAAGCTAATTTTTTTTATGAGAAACAACTGAGAAGACCATTAAATTTGTGTGAAATTTTCTTTCATTAGACTTAATGACCTCACAGTAGCTGCACCATGTAGTTGTTATTCATGTCCTTGAGAGAGATATCGATAGAATGCATGCAATATCTTATACTTTCACTAATTTTGTTTTTATTCTAAAAAACATTACTTCTATTATATTAAAGTAATGGTTATAAACTAGGATTAATCCAATTGTTAAGCAGTTTTATTTTCCTTTACATGACTTGAATATATATTTAAATAGTTAGCGTAACTTTTAAAAATGAATTTGTTATTATCTTTCTTAATGTTTTGTGTATGATGTGATAAGGTTATTTATACCGAAGGAGGACCAAAACGAATGTATCCTAGCATGAATGTTAAGCATCTGATACTGAAGACCGCTTTGCACCATTTTGAGTTTTTGGGAGTATCTTTTATGCTAAGAAGCTGCCCTTACTTAGAGCGTCTCACAATTGAAATTGTTGACGAAAGACACCTGTTTGTAAGTATTACATAAATTTGATTTAATTTAATTAATTTTCTCTCTCTATATATGTGTGTATTATGCATTTAATTTGGATACATTGTCGATGTAAAATATTTTTATACAGACATGTAGTCATTTTCGTTTTGAGGCAAATATCTAATCATAATTAACCAAATAGAAATTCATTTTGTTCTTGACCAAAATAATTTTAGTTTACTTTATTTTGTTTGGTTGGATTTCACTTGTATTTTTTTTCGTCAATGGGTTTGGTACCACTTATATTTTCTCTCAACAGAAAATAAAATTCATTATATATATATATATATATATATATATATATATATATATATAATTGTAGATTTTAGAAATTAACATAATGAATACCATAGCTTCTTTTTTTGGTTACAAGTATTATTTTTATGGAGAAATGTTAGGAATACTCTCTTTATAGCACTCTCTAACACTCACTTTTCTATTAGGTGAAACGCATGTGAATCTCACCACTTTATGTGAGATCCATTTCCAATGTGTGGTACCCACATGGATTTCACCCAAGCGAGTGTTAGAGAGAGTGTCCATATCACTCATCTTTTTTTGTTTATAAATATCATAGTTTAATTTGACTAAATATATGCATAAAAATATTATAATATTATTTTGTAATTGTGAATTTTTGAAGTAGGTAGGATACAAATCAAACTATTTTTATCAGTTGATGATCACAATTGATTGAGAGTCTAAACTTACTTGGCACATATAAATTAAATTTTATACTTCATATGCCTTTTGATATAAAATTAAAAACATGGAACTTTTTGTTGTTGTTTATATTGTAGGGTTATGAAGCAATGTTCAATGTTATCCCAGAGATATACTGGGTTGACTCTATAAAAGTTTACAAATGTGTGAAAAACTGTTTGAAGGAAATCGAGATTAAGAGCTTTAAGGGAACTAAAAATGAGTTGAACGTGATTACTTACTTTCTCACTTATGGAAAGGTCTTGAGAAAGTTGAGTATCAATTTCCTTAGAGATGAATGGAATGTCGCTTGTCCCCGTAAAGCAGTAGAGCATTTTCGAATGATTCCAAAAGCTTCAAATAATTTGGAGATATCAATCTATTAG
Protein sequence

>MS.gene21278.t1

MGMSNDILSSLPESLLSAIVSFLPFKEAVRTSILSKDWVSVYKSTRKIEFNELFFVNVDQSPEIVDAQRRAFSAFVRKWIEKHGEDVIDKFSLTIMLPEFFSELIDQCFRFALRNGIKDLELDFTYQPYDIVSAVVVLPTYFYGCSGFESLKLYACKFDASQCVNFHSLKKISLGYMGIKLHAIKALLTNCEKLECLSFLKCWNSEGDFILNEEYMGLKKLVINSCDFKFNIFRVNAPNLKVFNYHGLMILFAVDIKSPALDEVNLDFSLEHRGQGHGSYLYKFLKEFSSAKIMTVCSYLLQVIYTEGGPKRMYPSMNVKHLILKTALHHFEFLGVSFMLRSCPYLERLTIEIVDERHLFGYEAMFNVIPEIYWVDSIKVYKCVKNCLKEIEIKSFKGTKNELNVITYFLTYGKVLRKLSINFLRDEWNVACPRKAVEHFRMIPKASNNLEISIY