AlfalfaGEDB | MS.gene061531

M. sativa cultivar XinJiangDaYe / MS.gene061531

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08387.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061531.t1	XP_004490493.1	59.1	452	147	3	1	445	1	421	1.40E-143	519.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061531.t1	Q9C7X8	33.5	430	256	10	3	414	10	427	2.0e-56	221.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061531.t1	A0A1S2XMC6	59.1	452	147	3	1	445	1	421	1.0e-143	519.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061531	MS.gene69561	PPI
MS.gene061531	MS.gene045448	PPI
MS.gene21278	MS.gene061531	PPI
MS.gene061531	MS.gene34825	PPI
MS.gene061531	MS.gene032853	PPI
MS.gene061531	MS.gene003456	PPI
MS.gene99982	MS.gene061531	PPI
MS.gene061531	MS.gene062537	PPI
MS.gene34707	MS.gene061531	PPI
MS.gene061531	MS.gene99980	PPI
MS.gene061531	MS.gene25297	PPI
MS.gene63111	MS.gene061531	PPI
MS.gene23706	MS.gene061531	PPI
MS.gene072003	MS.gene061531	PPI
MS.gene061531	MS.gene29863	PPI
MS.gene061531	MS.gene52136	PPI
MS.gene34804	MS.gene061531	PPI
MS.gene061531	MS.gene09996	PPI
MS.gene061531	MS.gene63256	PPI
MS.gene061531	MS.gene34740	PPI
MS.gene061531	MS.gene032295	PPI
MS.gene061531	MS.gene061286	PPI
MS.gene73831	MS.gene061531	PPI
MS.gene25347	MS.gene061531	PPI
MS.gene64989	MS.gene061531	PPI
MS.gene061531	MS.gene024262	PPI
MS.gene061531	MS.gene98001	PPI
MS.gene061531	MS.gene94460	PPI
MS.gene033897	MS.gene061531	PPI
MS.gene061531	MS.gene64992	PPI
MS.gene34738	MS.gene061531	PPI
MS.gene25353	MS.gene061531	PPI
MS.gene032861	MS.gene061531	PPI
MS.gene061531	MS.gene031603	PPI
MS.gene25415	MS.gene061531	PPI
MS.gene061531	MS.gene75903	PPI
MS.gene061531	MS.gene031572	PPI
MS.gene061531	MS.gene73780	PPI
MS.gene36066	MS.gene061531	PPI
MS.gene75904	MS.gene061531	PPI
MS.gene061531	MS.gene045772	PPI
MS.gene061531	MS.gene045440	PPI
MS.gene061531	MS.gene031573	PPI
MS.gene061531	MS.gene006723	PPI
MS.gene061531	MS.gene028696	PPI
MS.gene63113	MS.gene061531	PPI
MS.gene006727	MS.gene061531	PPI
MS.gene63094	MS.gene061531	PPI
MS.gene006725	MS.gene061531	PPI
MS.gene061531	MS.gene25451	PPI
MS.gene29873	MS.gene061531	PPI
MS.gene63112	MS.gene061531	PPI
MS.gene061531	MS.gene041325	PPI
MS.gene061531	MS.gene95902	PPI
MS.gene061531	MS.gene069762	PPI
MS.gene061531	MS.gene51795	PPI
MS.gene031572	MS.gene061531	PPI
MS.gene061531	MS.gene34766	PPI
MS.gene061531	MS.gene074346	PPI
MS.gene63213	MS.gene061531	PPI
MS.gene91885	MS.gene061531	PPI
MS.gene041325	MS.gene061531	PPI
MS.gene34824	MS.gene061531	PPI
MS.gene64993	MS.gene061531	PPI
MS.gene20329	MS.gene061531	PPI
MS.gene01653	MS.gene061531	PPI
MS.gene96120	MS.gene061531	PPI
MS.gene061531	MS.gene003455	PPI
MS.gene047137	MS.gene061531	PPI
MS.gene25344	MS.gene061531	PPI
MS.gene34704	MS.gene061531	PPI
MS.gene061531	MS.gene010060	PPI
MS.gene061531	MS.gene002112	PPI
MS.gene061531	MS.gene040413	PPI
MS.gene061531	MS.gene09997	PPI
MS.gene94457	MS.gene061531	PPI
MS.gene045439	MS.gene061531	PPI
MS.gene20330	MS.gene061531	PPI
MS.gene061531	MS.gene63105	PPI
MS.gene061531	MS.gene006728	PPI
MS.gene34216	MS.gene061531	PPI
MS.gene006730	MS.gene061531	PPI
MS.gene041326	MS.gene061531	PPI
MS.gene061531	MS.gene63259	PPI
MS.gene061531	MS.gene60525	PPI
MS.gene72958	MS.gene061531	PPI
MS.gene047136	MS.gene061531	PPI
MS.gene045446	MS.gene061531	PPI
MS.gene75902	MS.gene061531	PPI
MS.gene061531	MS.gene007876	PPI
MS.gene22129	MS.gene061531	PPI
MS.gene061531	MS.gene033695	PPI
MS.gene061531	MS.gene045451	PPI
MS.gene061531	MS.gene005798	PPI
MS.gene061531	MS.gene34802	PPI
MS.gene061531	MS.gene25348	PPI
MS.gene061531	MS.gene031575	PPI
MS.gene061531	MS.gene54959	PPI
MS.gene061531	MS.gene34706	PPI
MS.gene91884	MS.gene061531	PPI
MS.gene061531	MS.gene66626	PPI
MS.gene78164	MS.gene061531	PPI
MS.gene061531	MS.gene25450	PPI
MS.gene045441	MS.gene061531	PPI
MS.gene061531	MS.gene29498	PPI
MS.gene061531	MS.gene045449	PPI
MS.gene006692	MS.gene061531	PPI
MS.gene061531	MS.gene50153	PPI
MS.gene061531	MS.gene64988	PPI
MS.gene94458	MS.gene061531	PPI
MS.gene053312	MS.gene061531	PPI
MS.gene061531	MS.gene035630	PPI
MS.gene061531	MS.gene031577	PPI
MS.gene73780	MS.gene061531	PPI
MS.gene061531	MS.gene069763	PPI
MS.gene79176	MS.gene061531	PPI
MS.gene061531	MS.gene97999	PPI
MS.gene061531	MS.gene053311	PPI
MS.gene061531	MS.gene25299	PPI
MS.gene72954	MS.gene061531	PPI
MS.gene021295	MS.gene061531	PPI
MS.gene049943	MS.gene061531	PPI
MS.gene061531	MS.gene005799	PPI
MS.gene061531	MS.gene25298	PPI
MS.gene061531	MS.gene25448	PPI
MS.gene055882	MS.gene061531	PPI
MS.gene061531	MS.gene006736	PPI
MS.gene91886	MS.gene061531	PPI
MS.gene061531	MS.gene033899	PPI
MS.gene032852	MS.gene061531	PPI
MS.gene061531	MS.gene021295	PPI
MS.gene069762	MS.gene061531	PPI
MS.gene072000	MS.gene061531	PPI
MS.gene061531	MS.gene20330	PPI
MS.gene065373	MS.gene061531	PPI
MS.gene061531	MS.gene045452	PPI
MS.gene63257	MS.gene061531	PPI
MS.gene055883	MS.gene061531	PPI
MS.gene061531	MS.gene031570	PPI
MS.gene061531	MS.gene34703	PPI
MS.gene061531	MS.gene041323	PPI
MS.gene032862	MS.gene061531	PPI
MS.gene061531	MS.gene25386	PPI
MS.gene061531	MS.gene63221	PPI
MS.gene96357	MS.gene061531	PPI
MS.gene071257	MS.gene061531	PPI
MS.gene061531	MS.gene51793	PPI
MS.gene54960	MS.gene061531	PPI
MS.gene63177	MS.gene061531	PPI
MS.gene25449	MS.gene061531	PPI
MS.gene033694	MS.gene061531	PPI
MS.gene061531	MS.gene60523	PPI
MS.gene061531	MS.gene055877	PPI
MS.gene061531	MS.gene63222	PPI
MS.gene061531	MS.gene25352	PPI
MS.gene045437	MS.gene061531	PPI
MS.gene061531	MS.gene63260	PPI
MS.gene34801	MS.gene061531	PPI
MS.gene061531	MS.gene032860	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061531.t1	MTR_5g080150	55.663	415	140	6	1	407	1	379	6.71e-142	412
MS.gene061531.t1	MTR_2g048140	39.912	456	260	8	6	454	7	455	1.55e-101	311
MS.gene061531.t1	MTR_1g090907	39.780	455	261	7	6	454	7	454	1.32e-99	306
MS.gene061531.t1	MTR_3g448340	35.849	477	262	12	1	452	1	458	2.63e-84	266
MS.gene061531.t1	MTR_1g090903	32.969	458	248	11	4	454	5	410	2.98e-63	210
MS.gene061531.t1	MTR_7g029260	61.905	126	48	0	302	427	34	159	1.11e-45	158
MS.gene061531.t1	MTR_3g049530	33.711	353	184	12	134	455	84	417	4.33e-41	151
MS.gene061531.t1	MTR_5g066890	70.930	86	23	1	317	402	1	84	1.47e-32	119
MS.gene061531.t1	MTR_5g066900	73.737	99	11	1	1	99	1	84	1.93e-31	115
MS.gene061531.t1	MTR_5g080160	52.525	99	41	1	362	454	61	159	4.03e-25	101
MS.gene061531.t1	MTR_5g080160	68.421	57	17	1	1	56	1	57	5.53e-14	70.1
MS.gene061531.t1	MTR_2g047858	25.234	428	268	14	6	421	115	502	1.20e-21	97.8
MS.gene061531.t1	MTR_5g080110	59.701	67	27	0	1	67	1	67	2.45e-17	76.6
MS.gene061531.t1	MTR_1g021825	23.570	437	256	16	6	421	88	467	1.24e-16	82.4
MS.gene061531.t1	MTR_1g021825	23.570	437	256	16	6	421	17	396	1.29e-16	82.0
MS.gene061531.t1	MTR_5g092960	23.840	474	307	16	6	455	19	462	2.12e-16	81.6
MS.gene061531.t1	MTR_8g468470	26.233	446	277	19	4	422	12	432	2.74e-16	81.3
MS.gene061531.t1	MTR_1g016340	23.990	421	283	12	6	419	20	410	4.06e-16	80.5
MS.gene061531.t1	MTR_1g016340	23.990	421	283	12	6	419	15	405	4.15e-16	80.5
MS.gene061531.t1	MTR_5g080090	50.667	75	27	2	331	404	39	104	1.62e-15	72.8
MS.gene061531.t1	MTR_2g072720	25.000	468	274	19	5	441	38	459	3.51e-15	77.8
MS.gene061531.t1	MTR_8g467800	24.263	441	276	15	5	422	19	424	8.42e-15	76.6
MS.gene061531.t1	MTR_4g032730	25.105	478	289	19	6	447	15	459	1.38e-14	75.9
MS.gene061531.t1	MTR_5g084070	23.841	453	292	11	6	449	13	421	1.74e-14	75.5
MS.gene061531.t1	MTR_8g468810	23.779	471	300	15	6	452	25	460	1.61e-13	72.8
MS.gene061531.t1	MTR_2g047872	21.596	426	283	11	6	421	23	407	1.89e-13	72.4
MS.gene061531.t1	MTR_1g013840	23.303	442	273	16	1	423	51	445	2.68e-13	72.0
MS.gene061531.t1	MTR_4g045900	22.363	474	291	17	6	421	26	480	2.90e-13	72.0
MS.gene061531.t1	MTR_8g014160	31.188	202	122	9	6	197	41	235	5.92e-13	70.9
MS.gene061531.t1	MTR_4g103670	23.235	439	254	18	6	422	25	402	1.05e-12	70.1
MS.gene061531.t1	MTR_5g080120	57.407	54	23	0	317	370	1	54	1.17e-12	63.2
MS.gene061531.t1	MTR_1g011400	21.689	438	271	12	6	421	7	394	1.81e-12	69.3
MS.gene061531.t1	MTR_0049s0110	28.205	195	127	6	6	199	8	190	2.06e-12	68.6
MS.gene061531.t1	MTR_0049s0110	28.205	195	127	6	6	199	8	190	2.08e-12	68.6
MS.gene061531.t1	MTR_0049s0110	28.205	195	127	6	6	199	8	190	2.25e-12	67.8
MS.gene061531.t1	MTR_0049s0110	28.205	195	127	6	6	199	8	190	2.72e-12	68.6
MS.gene061531.t1	MTR_2g091145	23.185	427	274	15	6	419	21	406	2.81e-12	68.9
MS.gene061531.t1	MTR_8g062010	25.806	465	275	22	4	447	7	422	3.28e-12	68.6
MS.gene061531.t1	MTR_2g072730	23.937	447	272	17	6	423	16	423	3.53e-12	68.6
MS.gene061531.t1	MTR_7g076760	23.148	432	278	16	6	419	21	416	5.31e-12	68.2
MS.gene061531.t1	MTR_5g040850	22.951	427	279	12	3	421	4	388	8.39e-12	67.4
MS.gene061531.t1	MTR_8g062270	24.512	461	299	19	6	447	16	446	1.07e-11	67.0
MS.gene061531.t1	MTR_4g088845	22.454	432	278	14	6	419	19	411	1.68e-11	66.6
MS.gene061531.t1	MTR_4g080590	22.378	429	288	14	6	421	23	419	2.03e-11	66.2
MS.gene061531.t1	MTR_8g445780	23.741	417	270	15	6	410	13	393	2.57e-11	65.5
MS.gene061531.t1	MTR_8g062310	25.486	463	265	19	2	447	21	420	2.99e-11	65.5
MS.gene061531.t1	MTR_1g071060	38.636	88	53	1	38	125	7	93	3.10e-11	60.1
MS.gene061531.t1	MTR_6g018660	24.599	374	223	12	2	344	16	361	3.39e-11	65.1
MS.gene061531.t1	MTR_8g070950	24.788	472	275	20	6	452	15	431	5.69e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061531.t1	AT1G56400	33.100	429	261	9	2	414	9	427	1.89e-65	218
MS.gene061531.t1	AT3G62230	30.258	466	305	8	2	455	4	461	8.88e-60	202
MS.gene061531.t1	AT5G54820	30.841	428	266	8	2	410	3	419	9.48e-59	200
MS.gene061531.t1	AT3G29830	29.274	468	300	12	6	455	8	462	1.24e-53	186
MS.gene061531.t1	AT3G29830	29.424	469	297	13	6	455	8	461	2.13e-53	186
MS.gene061531.t1	AT1G56400	31.469	429	227	10	2	414	9	386	4.45e-53	184
MS.gene061531.t1	AT1G61330	25.114	438	291	15	7	431	11	424	1.74e-24	105
MS.gene061531.t1	AT2G17305	31.548	168	105	3	294	455	106	269	2.82e-23	99.4
MS.gene061531.t1	AT3G58880	24.528	424	279	13	6	409	3	405	3.48e-18	87.0
MS.gene061531.t1	AT3G58860	24.292	424	291	12	6	421	7	408	2.13e-17	84.7
MS.gene061531.t1	AT4G26340	23.661	448	302	15	6	449	2	413	7.72e-16	79.7
MS.gene061531.t1	AT1G16930	21.519	474	307	11	4	452	13	446	1.03e-15	79.3
MS.gene061531.t1	AT1G16930	21.519	474	307	11	4	452	13	446	1.03e-15	79.3
MS.gene061531.t1	AT1G16930	21.519	474	307	11	4	452	13	446	1.03e-15	79.3
MS.gene061531.t1	AT5G44980	23.620	453	266	13	3	423	1	405	1.58e-15	79.0
MS.gene061531.t1	AT5G44980	23.620	453	266	13	3	423	1	405	1.58e-15	79.0
MS.gene061531.t1	AT3G28410	21.692	461	319	11	6	454	28	458	2.17e-15	78.6
MS.gene061531.t1	AT5G53635	23.973	438	277	14	7	422	1	404	2.43e-15	78.2
MS.gene061531.t1	AT3G52680	24.512	461	301	17	3	452	18	442	5.59e-15	77.4
MS.gene061531.t1	AT3G58900	23.918	439	294	11	6	421	2	423	7.03e-15	77.0
MS.gene061531.t1	AT3G42770	22.343	461	307	14	8	454	1	424	7.19e-15	77.0
MS.gene061531.t1	AT1G66310	22.698	467	299	15	6	454	19	441	1.08e-14	76.3
MS.gene061531.t1	AT1G32375	25.754	431	265	17	6	422	2	391	1.88e-14	75.5
MS.gene061531.t1	AT1G32375	25.754	431	265	17	6	422	2	391	1.88e-14	75.5
MS.gene061531.t1	AT3G58940	25.166	453	277	16	6	434	2	416	2.14e-14	75.9
MS.gene061531.t1	AT3G58940	25.166	453	277	16	6	434	2	416	2.14e-14	75.9
MS.gene061531.t1	AT5G53840	24.520	469	297	16	2	455	14	440	4.69e-14	74.3
MS.gene061531.t1	AT5G22730	22.098	448	280	13	6	423	28	436	4.74e-14	74.3
MS.gene061531.t1	AT5G22730	22.098	448	280	13	6	423	28	436	4.74e-14	74.3
MS.gene061531.t1	AT4G26350	23.918	439	261	16	6	418	2	393	1.34e-13	72.8
MS.gene061531.t1	AT5G22610	23.871	465	293	17	6	455	18	436	2.03e-13	72.4
MS.gene061531.t1	AT1G78760	21.810	431	287	13	6	421	16	411	5.94e-13	70.9
MS.gene061531.t1	AT3G59000	24.890	454	272	16	6	421	2	424	8.00e-13	70.9
MS.gene061531.t1	AT1G80470	23.207	474	289	20	5	454	14	436	8.54e-13	70.5
MS.gene061531.t1	AT1G69630	23.853	436	278	17	6	421	18	419	8.68e-13	70.5
MS.gene061531.t1	AT5G02930	24.390	369	224	11	6	351	28	364	1.01e-12	70.5
MS.gene061531.t1	AT4G00160	29.482	251	145	8	3	241	13	243	1.13e-12	70.1
MS.gene061531.t1	AT5G02910	25.698	358	230	11	6	354	11	341	1.53e-12	68.9
MS.gene061531.t1	AT5G02910	25.698	358	230	11	6	354	11	341	2.38e-12	68.6
MS.gene061531.t1	AT5G02910	25.698	358	230	11	6	354	11	341	2.62e-12	68.9
MS.gene061531.t1	AT3G58920	22.382	487	319	15	6	454	2	467	3.50e-12	68.6
MS.gene061531.t1	AT3G52680	26.367	311	201	10	3	308	18	305	3.85e-12	68.2
MS.gene061531.t1	AT1G13570	28.500	200	129	6	1	199	1	187	4.22e-12	67.0
MS.gene061531.t1	AT2G42730	22.340	470	324	18	4	454	3	450	4.30e-12	68.2
MS.gene061531.t1	AT3G59240	21.219	443	302	13	6	423	8	428	4.35e-12	68.6
MS.gene061531.t1	AT5G22670	22.697	445	278	15	4	423	9	412	7.25e-12	67.4
MS.gene061531.t1	AT1G13570	28.500	200	129	6	1	199	1	187	1.57e-11	66.2
MS.gene061531.t1	AT1G13570	28.500	200	129	6	1	199	1	187	1.57e-11	66.2
MS.gene061531.t1	AT4G14103	22.222	387	236	13	1	354	3	357	2.30e-11	65.9
MS.gene061531.t1	AT4G14103	27.670	206	124	6	1	197	3	192	2.72e-11	65.5
MS.gene061531.t1	AT4G14103	27.670	206	124	6	1	197	3	192	2.72e-11	65.5
MS.gene061531.t1	AT3G58900	26.749	243	169	4	6	245	2	238	3.05e-11	64.7
MS.gene061531.t1	AT3G58900	26.749	243	169	4	6	245	2	238	3.23e-11	64.7
MS.gene061531.t1	AT3G58900	26.749	243	169	4	6	245	2	238	3.23e-11	64.7
MS.gene061531.t1	AT4G13985	23.941	472	308	16	2	452	15	456	3.24e-11	65.5
MS.gene061531.t1	AT1G66290	20.601	466	315	18	2	452	25	450	7.62e-11	64.3

Find 60 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCAAAAGCTTCAAATAATT+TGG	0.116785	5.4:-52189956	MS.gene061531:CDS
CTTTGAGCATCAACAATTTC+AGG	0.151883	5.4:+52192951	None:intergenic
AATCGAGATTAAGAGCTTTA+AGG	0.179493	5.4:-52190115	MS.gene061531:CDS
TCCAAATTATTTGAAGCTTT+TGG	0.187191	5.4:+52189955	None:intergenic
ATTACTTACTTTCTCACTTA+TGG	0.189242	5.4:-52190069	MS.gene061531:CDS
AATTGAAAACCTTTAGATTT+GGG	0.237542	5.4:+52192424	None:intergenic
TTTGGAGATATCAATCTATT+AGG	0.275918	5.4:-52189938	None:intergenic
TAATTGAAAACCTTTAGATT+TGG	0.278932	5.4:+52192423	None:intergenic
ATGTTATCCCAGAGATATAC+TGG	0.291076	5.4:-52190185	MS.gene061531:CDS
AAGAAATGGAATCAAGGATT+TGG	0.294548	5.4:-52192796	MS.gene061531:CDS
GAAAATGCTCTACTGCTTTA+CGG	0.316225	5.4:+52189986	None:intergenic
ATCGAGATTAAGAGCTTTAA+GGG	0.324791	5.4:-52190114	MS.gene061531:CDS
AAAATGCTCTACTGCTTTAC+GGG	0.336374	5.4:+52189987	None:intergenic
CTTCTATTCTCTCTAAAGAT+TGG	0.337117	5.4:-52193043	MS.gene061531:CDS
GATAAAGCTACATGCTATTA+AGG	0.337780	5.4:-52192601	MS.gene061531:CDS
TTTCGTTTCGCTTTAAGAAA+TGG	0.339603	5.4:-52192810	MS.gene061531:CDS
ATACTCCCATAAACTCAAAA+TGG	0.346831	5.4:+52191479	None:intergenic
ATGCTAGGATACATTCGTTT+TGG	0.347569	5.4:+52191538	None:intergenic
CGACATTCCATTCATCTCTA+AGG	0.355244	5.4:+52190016	None:intergenic
TTTCAGCTTTCGTGAGGAAA+TGG	0.367314	5.4:-52192920	MS.gene061531:CDS
ATTGCCCGAATCATTGCTTT+CGG	0.390421	5.4:-52193105	MS.gene061531:CDS
GAGTCAACCCAGTATATCTC+TGG	0.398444	5.4:+52190177	None:intergenic
TTCTATTCTCTCTAAAGATT+GGG	0.400740	5.4:-52193042	MS.gene061531:CDS
TTTGACCAATTGTGAAAAGT+TGG	0.408885	5.4:-52192574	MS.gene061531:CDS
CGTATGCAGCTACTTGCTTC+AGG	0.420928	5.4:-52192250	MS.gene061531:intron
CGCTTTAAGAAATGGAATCA+AGG	0.433111	5.4:-52192802	MS.gene061531:CDS
TCAATTTCCTTAGAGATGAA+TGG	0.436949	5.4:-52190023	MS.gene061531:CDS
GATGGCCGAAAGCAATGATT+CGG	0.465360	5.4:+52193100	None:intergenic
ATTGTGAGACGCTCTAAGTA+AGG	0.466101	5.4:+52191436	None:intergenic
AGTAAATGCCCCAAATCTAA+AGG	0.472229	5.4:-52192433	MS.gene061531:CDS
TCCCTTGAGCATCGTGGTCA+AGG	0.474795	5.4:-52192330	MS.gene061531:CDS
TGATATTAAATCTCCTGCAT+TGG	0.479200	5.4:-52192373	MS.gene061531:CDS
ATGGCCGAAAGCAATGATTC+GGG	0.488444	5.4:+52193101	None:intergenic
ACTGCTGAAACAATATCATA+AGG	0.492198	5.4:+52192753	None:intergenic
AAAATTTCTTTGGGTTACAT+GGG	0.493426	5.4:-52192624	MS.gene061531:CDS
CGCTTCTTTGAACGGCAGAA+AGG	0.500724	5.4:+52193073	None:intergenic
AAATTGTTGATGCTCAAAGA+AGG	0.517077	5.4:-52192947	MS.gene061531:CDS
TAAATCTCCTGCATTGGATG+AGG	0.519266	5.4:-52192367	MS.gene061531:CDS
CTTTCTGCCGTTCAAAGAAG+CGG	0.527237	5.4:-52193072	MS.gene061531:CDS
TTGAAATGCTGGAATTCGGA+AGG	0.539765	5.4:-52192537	MS.gene061531:CDS
TTACTTTCTCACTTATGGAA+AGG	0.540191	5.4:-52190064	MS.gene061531:CDS
ATTGAAAACCTTTAGATTTG+GGG	0.540504	5.4:+52192425	None:intergenic
TGCAGGAGATTTAATATCAA+CGG	0.548324	5.4:+52192377	None:intergenic
GTCCTTGACCACGATGCTCA+AGG	0.552550	5.4:+52192328	None:intergenic
TATGTCATTGCTCATTCCCA+TGG	0.552959	5.4:+52193136	None:intergenic
GTTCTCACCGCTTCTTTGAA+CGG	0.559469	5.4:+52193065	None:intergenic
GAGCATCGTGGTCAAGGACA+TGG	0.568121	5.4:-52192324	MS.gene061531:CDS
AGATTAACCTCATCCAATGC+AGG	0.570665	5.4:+52192360	None:intergenic
TCCTTGACCACGATGCTCAA+GGG	0.583759	5.4:+52192329	None:intergenic
GCTAAGGTTATTTATACCGA+AGG	0.587879	5.4:-52191565	MS.gene061531:intron
AGATGCTTAACATTCATGCT+AGG	0.592384	5.4:+52191523	None:intergenic
AGTCAACCCAGTATATCTCT+GGG	0.594219	5.4:+52190178	None:intergenic
TTGTGAGACGCTCTAAGTAA+GGG	0.602951	5.4:+52191437	None:intergenic
CTGAAGCAAGTAGCTGCATA+CGG	0.604279	5.4:+52192251	None:intergenic
AAATTTCTTTGGGTTACATG+GGG	0.610716	5.4:-52192623	MS.gene061531:CDS
AATTGTTGATGCTCAAAGAA+GGG	0.622812	5.4:-52192946	MS.gene061531:CDS
TGTTATCCCAGAGATATACT+GGG	0.674333	5.4:-52190184	MS.gene061531:CDS
GAACGGCAGAAAGGAAACGA+TGG	0.683061	5.4:+52193082	None:intergenic
AAATGCTCTACTGCTTTACG+GGG	0.715301	5.4:+52189988	None:intergenic
AAACTCAAAATGGTGCAAAG+CGG	0.727187	5.4:+52191489	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATATATATATATATATAT+GGG	+	chr5.4:52192305-52192286	None:intergenic	0.0%
!!	TATATATATATATATATATA+TGG	+	chr5.4:52192306-52192287	None:intergenic	0.0%
!!	AAAATTCATTATATATATAG+GGG	-	chr5.4:52191973-52191992	MS.gene061531:intron	10.0%
!!	CAATTATATATATATATATG+AGG	+	chr5.4:52192367-52192386	None:intergenic	10.0%
!!	TTGATATAAAATTAAAAACA+TGG	-	chr5.4:52192819-52192838	MS.gene061531:CDS	10.0%
!!!	GTTTTTAATTTTATATCAAA+AGG	+	chr5.4:52192818-52192837	None:intergenic	10.0%
!!!	TATTACAAGTATTATTTTTA+TGG	-	chr5.4:52192431-52192450	MS.gene061531:CDS	10.0%
!!	AAAATTTCACACAAATTTAA+TGG	+	chr5.4:52191196-52191215	None:intergenic	15.0%
!!	AATTTAAGAAAAAAGACAAA+AGG	+	chr5.4:52190862-52190881	None:intergenic	15.0%
!!	TAAAGTAATGTTTATAAACT+AGG	-	chr5.4:52191361-52191380	MS.gene061531:intron	15.0%
!!!	AAAGTAAACTAAAAGTATTT+TGG	+	chr5.4:52191873-52191892	None:intergenic	15.0%
!!!	ATTTTGAATGAAGAATATAT+GGG	-	chr5.4:52190566-52190585	MS.gene061531:intron	15.0%
!!!	TATTTTGAATGAAGAATATA+TGG	-	chr5.4:52190565-52190584	MS.gene061531:intron	15.0%
!!!	TTAGTTTACTTTATTTTGTT+GGG	-	chr5.4:52191881-52191900	MS.gene061531:intron	15.0%
!!!	TTTAGTTTACTTTATTTTGT+TGG	-	chr5.4:52191880-52191899	MS.gene061531:intron	15.0%
!!	AAAGACAAAAGGTAAAAAAA+TGG	+	chr5.4:52190851-52190870	None:intergenic	20.0%
!!	AAATAAAACTGCTTAACAAT+TGG	+	chr5.4:52191393-52191412	None:intergenic	20.0%
!!	AATTGAAAACCTTTAGATTT+GGG	+	chr5.4:52190652-52190671	None:intergenic	20.0%
!!	ATCTCTATATTGTATTCATT+GGG	-	chr5.4:52191013-52191032	MS.gene061531:intron	20.0%
!!	GAACAAAATGAATTTCTATT+TGG	+	chr5.4:52191846-52191865	None:intergenic	20.0%
!!	TAATTGAAAACCTTTAGATT+TGG	+	chr5.4:52190653-52190672	None:intergenic	20.0%
!!	TATATATTCAAGTCATGTAA+AGG	+	chr5.4:52191417-52191436	None:intergenic	20.0%
!!	TCACTCAAAAAAATTTCTTT+GGG	-	chr5.4:52190440-52190459	MS.gene061531:intron	20.0%
!!	TGTAATAAAAAAAGAAGCTA+TGG	+	chr5.4:52192418-52192437	None:intergenic	20.0%
!!	TGTGTATTATGCATTTAATT+TGG	-	chr5.4:52191742-52191761	MS.gene061531:intron	20.0%
!!	TTCACTCAAAAAAATTTCTT+TGG	-	chr5.4:52190439-52190458	MS.gene061531:intron	20.0%
!!!	TAATTGTGAATTTTTGAAGT+AGG	-	chr5.4:52192693-52192712	MS.gene061531:CDS	20.0%
!!!	TTGTATTTTTTTTCGTCAAT+GGG	-	chr5.4:52191915-52191934	MS.gene061531:intron	20.0%
!!!	TTGTTGTTGTTTATATTGTA+GGG	-	chr5.4:52192848-52192867	MS.gene061531:CDS	20.0%
!!!	TTTGTTGTTGTTTATATTGT+AGG	-	chr5.4:52192847-52192866	MS.gene061531:CDS	20.0%
!!!	TTTTGAATGAAGAATATATG+GGG	-	chr5.4:52190567-52190586	MS.gene061531:intron	20.0%
!	ATATATGGGGTTGAAAAAAT+TGG	-	chr5.4:52190580-52190599	MS.gene061531:intron	25.0%
!	ATTACTTACTTTCTCACTTA+TGG	-	chr5.4:52193004-52193023	MS.gene061531:CDS	25.0%
!	ATTGAAAACCTTTAGATTTG+GGG	+	chr5.4:52190651-52190670	None:intergenic	25.0%
!	CTACAACCATAAAAATAAGT+AGG	+	chr5.4:52190356-52190375	None:intergenic	25.0%
!	GATCTCTATATTGTATTCAT+TGG	-	chr5.4:52191012-52191031	MS.gene061531:intron	25.0%
!	GCAAGTAAATAACTAAAAAG+TGG	-	chr5.4:52192039-52192058	MS.gene061531:intron	25.0%
!	TCCAAAAGCTTCAAATAATT+TGG	-	chr5.4:52193117-52193136	MS.gene061531:CDS	25.0%
!	TTTATGCAATACTTACAAAC+AGG	+	chr5.4:52191684-52191703	None:intergenic	25.0%
!!	AAAAATTTCTTTGGGTTACA+TGG	-	chr5.4:52190448-52190467	MS.gene061531:intron	25.0%
!!	AAAATTTCTTTGGGTTACAT+GGG	-	chr5.4:52190449-52190468	MS.gene061531:intron	25.0%
!!	CTAAAGGTTTTCAATTATCA+TGG	-	chr5.4:52190656-52190675	MS.gene061531:intron	25.0%
!!	TTCTATTCTCTCTAAAGATT+GGG	-	chr5.4:52190031-52190050	MS.gene061531:CDS	25.0%
!!!	ACTTATTTTTATGGTTGTAG+TGG	-	chr5.4:52190356-52190375	MS.gene061531:intron	25.0%
!!!	ATGTAGTCATATTTGTTTTG+AGG	-	chr5.4:52191799-52191818	MS.gene061531:intron	25.0%
!!!	CTAACACTCATTTTTCTATT+GGG	-	chr5.4:52192490-52192509	MS.gene061531:CDS	25.0%
!!!	CTTAGATTTCAAAGTTTTAG+AGG	-	chr5.4:52191114-52191133	MS.gene061531:intron	25.0%
!!!	CTTATTTTTATGGTTGTAGT+GGG	-	chr5.4:52190357-52190376	MS.gene061531:intron	25.0%
!!!	CTTGTATTTTTTTTCGTCAA+TGG	-	chr5.4:52191914-52191933	MS.gene061531:intron	25.0%
!!!	GTTTTGCCTACTTATTTTTA+TGG	-	chr5.4:52190347-52190366	MS.gene061531:intron	25.0%
!!!	TCCAAATTATTTGAAGCTTT+TGG	+	chr5.4:52193121-52193140	None:intergenic	25.0%
!!!	TCTAACACTCATTTTTCTAT+TGG	-	chr5.4:52192489-52192508	MS.gene061531:CDS	25.0%
!!!	TTTACTTTATTTTGTTGGGT+TGG	-	chr5.4:52191885-52191904	MS.gene061531:intron	25.0%
!!!	TTTTATTGTTTTGCTCTCTT+GGG	-	chr5.4:52191046-52191065	MS.gene061531:intron	25.0%
!!!	TTTTTATTGTTTTGCTCTCT+TGG	-	chr5.4:52191045-52191064	MS.gene061531:intron	25.0%
!!!	TTTTTTGAAATGCTGGAATT+CGG	-	chr5.4:52190532-52190551	MS.gene061531:intron	25.0%
	AAATTGTTGATGCTCAAAGA+AGG	-	chr5.4:52190126-52190145	MS.gene061531:CDS	30.0%
	AAGAAATGGAATCAAGGATT+TGG	-	chr5.4:52190277-52190296	MS.gene061531:intron	30.0%
	AATCGAGATTAAGAGCTTTA+AGG	-	chr5.4:52192958-52192977	MS.gene061531:CDS	30.0%
	AATTGTTGATGCTCAAAGAA+GGG	-	chr5.4:52190127-52190146	MS.gene061531:CDS	30.0%
	ACAAAAAAAGACGAGTGATA+TGG	+	chr5.4:52192611-52192630	None:intergenic	30.0%
	ACCTTCTAACAAAAACAAGT+GGG	-	chr5.4:52192097-52192116	MS.gene061531:intron	30.0%
	ACTGCTGAAACAATATCATA+AGG	+	chr5.4:52190323-52190342	None:intergenic	30.0%
	ATACTCCCATAAACTCAAAA+TGG	+	chr5.4:52191597-52191616	None:intergenic	30.0%
	ATGAGCTCACTAAAAAATTC+AGG	+	chr5.4:52190233-52190252	None:intergenic	30.0%
	ATGTGTGAAAAACTGTTTGA+AGG	-	chr5.4:52192934-52192953	MS.gene061531:CDS	30.0%
	CTGATGAGAGAAAATATAAG+TGG	+	chr5.4:52191948-52191967	None:intergenic	30.0%
	GATAAAGCTACATGCTATTA+AGG	-	chr5.4:52190472-52190491	MS.gene061531:intron	30.0%
	TCAATTTCCTTAGAGATGAA+TGG	-	chr5.4:52193050-52193069	MS.gene061531:CDS	30.0%
	TGATATTAAATCTCCTGCAT+TGG	-	chr5.4:52190700-52190719	MS.gene061531:intron	30.0%
	TGATGATCACAATTGATTGA+GGG	-	chr5.4:52192746-52192765	MS.gene061531:CDS	30.0%
	TGCAGGAGATTTAATATCAA+CGG	+	chr5.4:52190699-52190718	None:intergenic	30.0%
	TTACTTTCTCACTTATGGAA+AGG	-	chr5.4:52193009-52193028	MS.gene061531:CDS	30.0%
	TTATAGCAACATACACCTTA+GGG	+	chr5.4:52192074-52192093	None:intergenic	30.0%
	TTTGACCAATTGTGAAAAGT+TGG	-	chr5.4:52190499-52190518	MS.gene061531:intron	30.0%
!	AAATTTCTTTGGGTTACATG+GGG	-	chr5.4:52190450-52190469	MS.gene061531:intron	30.0%
!	ATCGAGATTAAGAGCTTTAA+GGG	-	chr5.4:52192959-52192978	MS.gene061531:CDS	30.0%
!	CTTCTATTCTCTCTAAAGAT+TGG	-	chr5.4:52190030-52190049	MS.gene061531:CDS	30.0%
!	TTGATGATCACAATTGATTG+AGG	-	chr5.4:52192745-52192764	MS.gene061531:CDS	30.0%
!!	AGGAAATGGATTGAAAAACA+CGG	-	chr5.4:52190167-52190186	MS.gene061531:CDS	30.0%
!!	TTTCGTTTCGCTTTAAGAAA+TGG	-	chr5.4:52190263-52190282	MS.gene061531:intron	30.0%
!!!	GATTTCAAAGTTTTAGAGGA+TGG	-	chr5.4:52191118-52191137	MS.gene061531:intron	30.0%
!!!	GTTTGAGTTTTTTGAAATGC+TGG	-	chr5.4:52190525-52190544	MS.gene061531:intron	30.0%
!!!	TGTGAATTTTTGAAGTAGGT+AGG	-	chr5.4:52192697-52192716	MS.gene061531:CDS	30.0%
!!!	TTGCACCATTTTGAGTTTAT+GGG	-	chr5.4:52191589-52191608	MS.gene061531:intron	30.0%
!!!	TTTGCACCATTTTGAGTTTA+TGG	-	chr5.4:52191588-52191607	MS.gene061531:intron	30.0%
!!!	TTTTTTTTCGTCAATGGGTT+TGG	-	chr5.4:52191920-52191939	MS.gene061531:intron	30.0%
	AAAATGCTCTACTGCTTTAC+GGG	+	chr5.4:52193089-52193108	None:intergenic	35.0%
	AAACTCAAAATGGTGCAAAG+CGG	+	chr5.4:52191587-52191606	None:intergenic	35.0%
	AAGTGGTTAAATACACCCTA+AGG	-	chr5.4:52192056-52192075	MS.gene061531:intron	35.0%
	AAGTGGTTAATTACACCCTA+AGG	-	chr5.4:52192205-52192224	MS.gene061531:intron	35.0%
	ACTCCCACATAAAAACTAGT+GGG	-	chr5.4:52192245-52192264	MS.gene061531:intron	35.0%
	ACTCCCACATAAAAACTAGT+GGG	+	chr5.4:52192264-52192245	None:intergenic	35.0%
	AGATGCTTAACATTCATGCT+AGG	+	chr5.4:52191553-52191572	None:intergenic	35.0%
	AGTAAATGCCCCAAATCTAA+AGG	-	chr5.4:52190640-52190659	MS.gene061531:intron	35.0%
	ATGTTATCCCAGAGATATAC+TGG	-	chr5.4:52192888-52192907	MS.gene061531:CDS	35.0%
	ATTGAGGGTCTAAACTTACT+TGG	-	chr5.4:52192761-52192780	MS.gene061531:CDS	35.0%
	CACCTTCTAACAAAAACAAG+TGG	-	chr5.4:52192096-52192115	MS.gene061531:intron	35.0%
	CTTTGAGCATCAACAATTTC+AGG	+	chr5.4:52190125-52190144	None:intergenic	35.0%
	GAAAATGCTCTACTGCTTTA+CGG	+	chr5.4:52193090-52193109	None:intergenic	35.0%
	GAAATGGATCTCACATAAAG+TGG	+	chr5.4:52192536-52192555	None:intergenic	35.0%
	GCTAAGGTTATTTATACCGA+AGG	-	chr5.4:52191508-52191527	MS.gene061531:CDS	35.0%
	GGACATGAATAACAACTACA+TGG	+	chr5.4:52191254-52191273	None:intergenic	35.0%
	GTGTTCTAGCAAATGCTTAT+AGG	+	chr5.4:52192138-52192157	None:intergenic	35.0%
	GTTATAGCAACATACACCTT+AGG	+	chr5.4:52192075-52192094	None:intergenic	35.0%
	TGTTATCCCAGAGATATACT+GGG	-	chr5.4:52192889-52192908	MS.gene061531:CDS	35.0%
	TTCTATCGATATCTCTCTCA+AGG	+	chr5.4:52191275-52191294	None:intergenic	35.0%
	TTTAGCAAAGCTACACCTTA+GGG	+	chr5.4:52192223-52192242	None:intergenic	35.0%
!	ACACTCCAACTTTTCACAAT+TGG	+	chr5.4:52190507-52190526	None:intergenic	35.0%
!	ATGCTAGGATACATTCGTTT+TGG	+	chr5.4:52191538-52191557	None:intergenic	35.0%
!	GTTTTGTGTATGATGTGCTA+AGG	-	chr5.4:52191492-52191511	MS.gene061531:CDS	35.0%
!	TTCGTGTATTTTGCTTGGTT+GGG	-	chr5.4:52191069-52191088	MS.gene061531:intron	35.0%
!	TTTTAGCAAAGCTACACCTT+AGG	+	chr5.4:52192224-52192243	None:intergenic	35.0%
!!	CGCTTTAAGAAATGGAATCA+AGG	-	chr5.4:52190271-52190290	MS.gene061531:intron	35.0%
!!!	ACACCCACTAGTTTTTATGT+GGG	-	chr5.4:52192156-52192175	MS.gene061531:intron	35.0%
!!!	ACACCCACTAGTTTTTATGT+GGG	+	chr5.4:52192175-52192156	None:intergenic	35.0%
!!!	ACCCACTTGTTTTTGTTAGA+AGG	+	chr5.4:52192101-52192120	None:intergenic	35.0%
	AAATGCTCTACTGCTTTACG+GGG	+	chr5.4:52193088-52193107	None:intergenic	40.0%
	ACTCCCACATGAAAACTAGT+GGG	+	chr5.4:52192013-52192032	None:intergenic	40.0%
	AGATTAACCTCATCCAATGC+AGG	+	chr5.4:52190716-52190735	None:intergenic	40.0%
	AGTCAACCCAGTATATCTCT+GGG	+	chr5.4:52192898-52192917	None:intergenic	40.0%
	ATTGCCCGAATCATTGCTTT+CGG	-	chr5.4:52189968-52189987	MS.gene061531:CDS	40.0%
	CACTCCCACATAAAAACTAG+TGG	-	chr5.4:52192244-52192263	MS.gene061531:intron	40.0%
	CACTCCCACATAAAAACTAG+TGG	+	chr5.4:52192263-52192244	None:intergenic	40.0%
	CGACATTCCATTCATCTCTA+AGG	+	chr5.4:52193060-52193079	None:intergenic	40.0%
	TAAATCTCCTGCATTGGATG+AGG	-	chr5.4:52190706-52190725	MS.gene061531:intron	40.0%
	TGAGATCCATTTCCAATGTG+CGG	-	chr5.4:52192543-52192562	MS.gene061531:CDS	40.0%
	TTGAAATGCTGGAATTCGGA+AGG	-	chr5.4:52190536-52190555	MS.gene061531:intron	40.0%
	TTTCAGCTTTCGTGAGGAAA+TGG	-	chr5.4:52190153-52190172	MS.gene061531:CDS	40.0%
!	ATTGTGAGACGCTCTAAGTA+AGG	+	chr5.4:52191640-52191659	None:intergenic	40.0%
!	GTTCGTGTATTTTGCTTGGT+TGG	-	chr5.4:52191068-52191087	MS.gene061531:intron	40.0%
!	TTGGGTTCGTGTATTTTGCT+TGG	-	chr5.4:52191064-52191083	MS.gene061531:intron	40.0%
!	TTGTGAGACGCTCTAAGTAA+GGG	+	chr5.4:52191639-52191658	None:intergenic	40.0%
!!!	ACACCCACTAGTTTTCATGT+GGG	-	chr5.4:52192007-52192026	MS.gene061531:intron	40.0%
!!!	CACACCCACTAGTTTTTATG+TGG	-	chr5.4:52192155-52192174	MS.gene061531:intron	40.0%
!!!	CACACCCACTAGTTTTTATG+TGG	+	chr5.4:52192174-52192155	None:intergenic	40.0%
	ACTCTCTCTAACACTCGCTT+GGG	+	chr5.4:52192586-52192605	None:intergenic	45.0%
	ATGGCCGAAAGCAATGATTC+GGG	+	chr5.4:52189975-52189994	None:intergenic	45.0%
	CACTCCCACATGAAAACTAG+TGG	+	chr5.4:52192014-52192033	None:intergenic	45.0%
	CTGAAGCAAGTAGCTGCATA+CGG	+	chr5.4:52190825-52190844	None:intergenic	45.0%
	CTTTCTGCCGTTCAAAGAAG+CGG	-	chr5.4:52190001-52190020	MS.gene061531:CDS	45.0%
	GAGTCAACCCAGTATATCTC+TGG	+	chr5.4:52192899-52192918	None:intergenic	45.0%
	GATGGCCGAAAGCAATGATT+CGG	+	chr5.4:52189976-52189995	None:intergenic	45.0%
	GTTCTCACCGCTTCTTTGAA+CGG	+	chr5.4:52190011-52190030	None:intergenic	45.0%
	TGCTTGGTTGGGCAGTAAAA+AGG	-	chr5.4:52191080-52191099	MS.gene061531:intron	45.0%
!!	CATTCGTTTTGGTCGTCCTT+CGG	+	chr5.4:52191527-52191546	None:intergenic	45.0%
!!!	CACACCCACTAGTTTTCATG+TGG	-	chr5.4:52192006-52192025	MS.gene061531:intron	45.0%
!!	TAAAATTCATTATATATATA+GGG	-	chr5.4:52191972-52191991	MS.gene061531:intron	5.0%
!!	TATATATATATATATATATG+GGG	+	chr5.4:52192285-52192304	None:intergenic	5.0%
!!	TTAAAATTCATTATATATAT+AGG	-	chr5.4:52191971-52191990	MS.gene061531:intron	5.0%
	CACTCTCTCTAACACTCGCT+TGG	+	chr5.4:52192587-52192606	None:intergenic	50.0%
	CGCTTCTTTGAACGGCAGAA+AGG	+	chr5.4:52190003-52190022	None:intergenic	50.0%
	CGCTTGGGTGAAATCCATGT+GGG	+	chr5.4:52192571-52192590	None:intergenic	50.0%
	CGTATGCAGCTACTTGCTTC+AGG	-	chr5.4:52190823-52190842	MS.gene061531:intron	50.0%
	GAACGGCAGAAAGGAAACGA+TGG	+	chr5.4:52189994-52190013	None:intergenic	50.0%
	TACATGGTGCAGCTACTGTG+AGG	+	chr5.4:52191238-52191257	None:intergenic	50.0%
	TCCTTGACCACGATGCTCAA+GGG	+	chr5.4:52190747-52190766	None:intergenic	50.0%
	TCGCTTGGGTGAAATCCATG+TGG	+	chr5.4:52192572-52192591	None:intergenic	50.0%
!	GGGCATTTTCAGCTTTCGTG+AGG	-	chr5.4:52190147-52190166	MS.gene061531:CDS	50.0%
!	TGGGTACCGCACATTGGAAA+TGG	+	chr5.4:52192552-52192571	None:intergenic	50.0%
!!	GACTTTTCCCTTGAGCATCG+TGG	-	chr5.4:52190737-52190756	MS.gene061531:intron	50.0%
	GTCCTTGACCACGATGCTCA+AGG	+	chr5.4:52190748-52190767	None:intergenic	55.0%
	TCCAATGTGCGGTACCCACA+TGG	-	chr5.4:52192554-52192573	MS.gene061531:CDS	55.0%
!	GAGCATCGTGGTCAAGGACA+TGG	-	chr5.4:52190749-52190768	MS.gene061531:intron	55.0%
!	TCCATGTGGGTACCGCACAT+TGG	+	chr5.4:52192558-52192577	None:intergenic	55.0%
!	TCCCTTGAGCATCGTGGTCA+AGG	-	chr5.4:52190743-52190762	MS.gene061531:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	52189939	52193156	52189939	ID=MS.gene061531
chr5.4	mRNA	52189939	52193156	52189939	ID=MS.gene061531.t1;Parent=MS.gene061531
chr5.4	exon	52192251	52193156	52192251	ID=MS.gene061531.t1.exon1;Parent=MS.gene061531.t1
chr5.4	CDS	52192251	52193156	52192251	ID=cds.MS.gene061531.t1;Parent=MS.gene061531.t1
chr5.4	exon	52191408	52191581	52191408	ID=MS.gene061531.t1.exon2;Parent=MS.gene061531.t1
chr5.4	CDS	52191408	52191581	52191408	ID=cds.MS.gene061531.t1;Parent=MS.gene061531.t1
chr5.4	exon	52189939	52190226	52189939	ID=MS.gene061531.t1.exon3;Parent=MS.gene061531.t1
chr5.4	CDS	52189939	52190226	52189939	ID=cds.MS.gene061531.t1;Parent=MS.gene061531.t1

Gene Sequence

>MS.gene061531

ATGGGAATGAGCAATGACATATTGAGTTCATTGCCCGAATCATTGCTTTCGGCCATCGTTTCCTTTCTGCCGTTCAAAGAAGCGGTGAGAACTTCTATTCTCTCTAAAGATTGGGTCAATGTATACAAATCAACAAGAAAAATAGAGTTCAATGAACTCTTCTTTGTTAACGTTGATCAATCTCCTGAAATTGTTGATGCTCAAAGAAGGGCATTTTCAGCTTTCGTGAGGAAATGGATTGAAAAACACGGAGAAGATGTCATTGACAAATTCTCTTTAACAATTATGTTGCCTGAATTTTTTAGTGAGCTCATTGATCAATGTTTTCGTTTCGCTTTAAGAAATGGAATCAAGGATTTGGAGCTTGATTTCACTTATCAACCTTATGATATTGTTTCAGCAGTAGTTGTTTTGCCTACTTATTTTTATGGTTGTAGTGGGTTTGAATCTTTGAAATTGTATGCATGTAAATTTGATGCTAGTCAATGTGTTAATTTTCATTCACTCAAAAAAATTTCTTTGGGTTACATGGGGATAAAGCTACATGCTATTAAGGCACTTTTGACCAATTGTGAAAAGTTGGAGTGTTTGAGTTTTTTGAAATGCTGGAATTCGGAAGGAGATTTTATTTTGAATGAAGAATATATGGGGTTGAAAAAATTGGTTATTAATAGCTGCGATTTTAAGTTTAATATTTTTAGAGTAAATGCCCCAAATCTAAAGGTTTTCAATTATCATGGATTGATGATACTTTTTGCCGTTGATATTAAATCTCCTGCATTGGATGAGGTTAATCTTGACTTTTCCCTTGAGCATCGTGGTCAAGGACATGGTTCTTATCTTTACAAATTTTTAAAAGAGTTCTCTTCTGCTAAAATTATGACCGTATGCAGCTACTTGCTTCAGGTACCATTTTTTTACCTTTTGTCTTTTTTCTTAAATTTCAAATTTCTTTTTGATAATCAATTTAATTATTATTGCACAATAGATTGCTTTTACTGTATTAATTAACTTTATTCGTTGAAACAATTTATAGCTAGATCTACATATTATTCTTCTCTAATTGTTTTGAAGATCTCTATATTGTATTCATTGGGTTTTTTTTTTTTTTATTGTTTTGCTCTCTTGGGTTCGTGTATTTTGCTTGGTTGGGCAGTAAAAAGGTATATATTTTTCTTAGATTTCAAAGTTTTAGAGGATGGCTACGTTAAAAATTCTAAAGCTAAATTTTTATATGAGAAACAACTGAGAAGACCATTAAATTTGTGTGAAATTTTCTTTCATTAGACTTAATGACCTCACAGTAGCTGCACCATGTAGTTGTTATTCATGTCCTTGAGAGAGATATCGATAGAATGCATGCAATATCTTATACTTTCACTAATTTTGTTTTTATTCTAAAAAACATTACTTCTATTATATTAAAGTAATGTTTATAAACTAGGATTAGTCCAATTGTTAAGCAGTTTTATTTTCCTTTACATGACTTGAATATATATATTTAAATAGTTAGCGTAACTTTTAAAAATGAATTTGTTATTATCTTTCTTAATGTTTTGTGTATGATGTGCTAAGGTTATTTATACCGAAGGACGACCAAAACGAATGTATCCTAGCATGAATGTTAAGCATCTGATACTGAAGACCGCTTTGCACCATTTTGAGTTTATGGGAGTATCTTTTATGCTAAGAAGCTGCCCTTACTTAGAGCGTCTCACAATTGAAATTGTTGACGAAAGATACCTGTTTGTAAGTATTGCATAAATTTGATTTAATTTAATTAATTTTCTCTCTCTATATGTGTGTGTATTATGCATTTAATTTGGATACATTGTCGATGTAAAATACTTTTATACAGACATGTAGTCATATTTGTTTTGAGGCAAATATCTAATCATAATTGACCAAATAGAAATTCATTTTGTTCTTGACCAAAATACTTTTAGTTTACTTTATTTTGTTGGGTTGGATTTCACTTGTATTTTTTTTCGTCAATGGGTTTGGTACCACTTATATTTTCTCTCATCAGAAATTAAAATTCATTATATATATAGGGGTTTGCTAGAACACACCCACTAGTTTTCATGTGGGAGTGTTTTAGCAAGTAAATAACTAAAAAGTGGTTAAATACACCCTAAGGTGTATGTTGCTATAACACACCTTCTAACAAAAACAAGTGGGTGTGTTCTAGCAAATCCTATAAGCATTTGCTAGAACACACCCACTAGTTTTTATGTGGGAGTGTTTTAGCAAGTAAATAACTAAAAAGTGGTTAATTACACCCTAAGGTGTAGCTTTGCTAAAACACTCCCACATAAAAACTAGTGGGTGTGTTCTAGCAAACCCCATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATACATCCTCATATATATATATATAATTGTAGATTTTAGAAATTAACATAATGAATACCATAGCTTCTTTTTTTATTACAAGTATTATTTTTATGGAGAAATGTTAGAAATACTCTCTTTATAGCATTCTCTCTAACACTCATTTTTCTATTGGGTGAAACTCATGCGAGTCTCACCACTTTATGTGAGATCCATTTCCAATGTGCGGTACCCACATGGATTTCACCCAAGCGAGTGTTAGAGAGAGTGTCCATATCACTCGTCTTTTTTTGTTTATAAATGTCATAGTTTAATTTGACTAAATATATGCATAAAAATATTATAATATTATTTTTTAATTGTGAATTTTTGAAGTAGGTAGGATAAAAATCAAACTATTTTTATCAGTTGATGATCACAATTGATTGAGGGTCTAAACTTACTTGGCACATATAAATTAAATTTTATACTTCATATGCCTTTTGATATAAAATTAAAAACATGGAACTTTTTGTTGTTGTTTATATTGTAGGGTTATGAAGCAATGTTCAATGTTATCCCAGAGATATACTGGGTTGACTCTATAAAAGTTTACAAATGTGTGAAAAACTGTTTGAAGGAAATCGAGATTAAGAGCTTTAAGGGAACTAAAAATGAGTTGAACGTGATTACTTACTTTCTCACTTATGGAAAGGTCTTGAGAAAGTTGAGTATCAATTTCCTTAGAGATGAATGGAATGTCGCTTGTCCCCGTAAAGCAGTAGAGCATTTTCGAATGATTCCAAAAGCTTCAAATAATTTGGAGATATCAATCTATTAG

Protein sequence

>MS.gene061531.t1

MGMSNDILSSLPESLLSAIVSFLPFKEAVRTSILSKDWVNVYKSTRKIEFNELFFVNVDQSPEIVDAQRRAFSAFVRKWIEKHGEDVIDKFSLTIMLPEFFSELIDQCFRFALRNGIKDLELDFTYQPYDIVSAVVVLPTYFYGCSGFESLKLYACKFDASQCVNFHSLKKISLGYMGIKLHAIKALLTNCEKLECLSFLKCWNSEGDFILNEEYMGLKKLVINSCDFKFNIFRVNAPNLKVFNYHGLMILFAVDIKSPALDEVNLDFSLEHRGQGHGSYLYKFLKEFSSAKIMTVCSYLLQVIYTEGRPKRMYPSMNVKHLILKTALHHFEFMGVSFMLRSCPYLERLTIEIVDERYLFGYEAMFNVIPEIYWVDSIKVYKCVKNCLKEIEIKSFKGTKNELNVITYFLTYGKVLRKLSINFLRDEWNVACPRKAVEHFRMIPKASNNLEISIY