AlfalfaGEDB | MS.gene22129

M. sativa cultivar XinJiangDaYe / MS.gene22129

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08387.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22129.t1	XP_024634531.1	89.1	403	44	0	1	403	1	403	2.10E-199	704.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22129.t1	Q9LJ48	27.2	434	232	15	6	367	8	429	4.3e-31	137.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22129.t1	A0A072UVY3	77.7	462	44	1	1	403	1	462	2.0e-191	677.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene22129	MS.gene003455	PPI
MS.gene22129	MS.gene035630	PPI
MS.gene91884	MS.gene22129	PPI
MS.gene22129	MS.gene031577	PPI
MS.gene22129	MS.gene069763	PPI
MS.gene22129	MS.gene97999	PPI
MS.gene22129	MS.gene053311	PPI
MS.gene22129	MS.gene25299	PPI
MS.gene045441	MS.gene22129	PPI
MS.gene91885	MS.gene22129	PPI
MS.gene006692	MS.gene22129	PPI
MS.gene22129	MS.gene63259	PPI
MS.gene96120	MS.gene22129	PPI
MS.gene22129	MS.gene006736	PPI
MS.gene053312	MS.gene22129	PPI
MS.gene047137	MS.gene22129	PPI
MS.gene22129	MS.gene033899	PPI
MS.gene22129	MS.gene007876	PPI
MS.gene22129	MS.gene021295	PPI
MS.gene22129	MS.gene005798	PPI
MS.gene94457	MS.gene22129	PPI
MS.gene22129	MS.gene78164	PPI
MS.gene22129	MS.gene25348	PPI
MS.gene049943	MS.gene22129	PPI
MS.gene006730	MS.gene22129	PPI
MS.gene22129	MS.gene031570	PPI
MS.gene72958	MS.gene22129	PPI
MS.gene22129	MS.gene25386	PPI
MS.gene22129	MS.gene63221	PPI
MS.gene22129	MS.gene25450	PPI
MS.gene22129	MS.gene63257	PPI
MS.gene22129	MS.gene63111	PPI
MS.gene22129	MS.gene34804	PPI
MS.gene22129	MS.gene60523	PPI
MS.gene22129	MS.gene055877	PPI
MS.gene22129	MS.gene25352	PPI
MS.gene22129	MS.gene01653	PPI
MS.gene22129	MS.gene045448	PPI
MS.gene22129	MS.gene34825	PPI
MS.gene22129	MS.gene032853	PPI
MS.gene22129	MS.gene003456	PPI
MS.gene22129	MS.gene062537	PPI
MS.gene22129	MS.gene99980	PPI
MS.gene22129	MS.gene005799	PPI
MS.gene22129	MS.gene25298	PPI
MS.gene22129	MS.gene25448	PPI
MS.gene22129	MS.gene29863	PPI
MS.gene22129	MS.gene52136	PPI
MS.gene94458	MS.gene22129	PPI
MS.gene22129	MS.gene25344	PPI
MS.gene045437	MS.gene22129	PPI
MS.gene22129	MS.gene09996	PPI
MS.gene22129	MS.gene34740	PPI
MS.gene22129	MS.gene64988	PPI
MS.gene79176	MS.gene22129	PPI
MS.gene22129	MS.gene25347	PPI
MS.gene22129	MS.gene20330	PPI
MS.gene22129	MS.gene061286	PPI
MS.gene21278	MS.gene22129	PPI
MS.gene22129	MS.gene024262	PPI
MS.gene22129	MS.gene045452	PPI
MS.gene22129	MS.gene98001	PPI
MS.gene22129	MS.gene75904	PPI
MS.gene22129	MS.gene34703	PPI
MS.gene23706	MS.gene22129	PPI
MS.gene22129	MS.gene94460	PPI
MS.gene22129	MS.gene041323	PPI
MS.gene072003	MS.gene22129	PPI
MS.gene22129	MS.gene64992	PPI
MS.gene91886	MS.gene22129	PPI
MS.gene032852	MS.gene22129	PPI
MS.gene22129	MS.gene031603	PPI
MS.gene22129	MS.gene51793	PPI
MS.gene22129	MS.gene031572	PPI
MS.gene22129	MS.gene73780	PPI
MS.gene22129	MS.gene72954	PPI
MS.gene64989	MS.gene22129	PPI
MS.gene22129	MS.gene63222	PPI
MS.gene055883	MS.gene22129	PPI
MS.gene22129	MS.gene63260	PPI
MS.gene22129	MS.gene033694	PPI
MS.gene22129	MS.gene032860	PPI
MS.gene22129	MS.gene045772	PPI
MS.gene22129	MS.gene061531	PPI
MS.gene033897	MS.gene22129	PPI
MS.gene032862	MS.gene22129	PPI
MS.gene22129	MS.gene69561	PPI
MS.gene22129	MS.gene006723	PPI
MS.gene22129	MS.gene028696	PPI
MS.gene34738	MS.gene22129	PPI
MS.gene96357	MS.gene22129	PPI
MS.gene071257	MS.gene22129	PPI
MS.gene22129	MS.gene25297	PPI
MS.gene22129	MS.gene25451	PPI
MS.gene54960	MS.gene22129	PPI
MS.gene22129	MS.gene006725	PPI
MS.gene22129	MS.gene94457	PPI
MS.gene63177	MS.gene22129	PPI
MS.gene25449	MS.gene22129	PPI
MS.gene22129	MS.gene069762	PPI
MS.gene34801	MS.gene22129	PPI
MS.gene22129	MS.gene51795	PPI
MS.gene22129	MS.gene63256	PPI
MS.gene22129	MS.gene032295	PPI
MS.gene22129	MS.gene055882	PPI
MS.gene22129	MS.gene074346	PPI
MS.gene63113	MS.gene22129	PPI
MS.gene99982	MS.gene22129	PPI
MS.gene34707	MS.gene22129	PPI
MS.gene22129	MS.gene63112	PPI
MS.gene22129	MS.gene010060	PPI
MS.gene22129	MS.gene002112	PPI
MS.gene22129	MS.gene047136	PPI
MS.gene22129	MS.gene040413	PPI
MS.gene22129	MS.gene09997	PPI
MS.gene73831	MS.gene22129	PPI
MS.gene22129	MS.gene75903	PPI
MS.gene22129	MS.gene63105	PPI
MS.gene22129	MS.gene006728	PPI
MS.gene63213	MS.gene22129	PPI
MS.gene34824	MS.gene22129	PPI
MS.gene64993	MS.gene22129	PPI
MS.gene20329	MS.gene22129	PPI
MS.gene22129	MS.gene065373	PPI
MS.gene22129	MS.gene045440	PPI
MS.gene22129	MS.gene031573	PPI
MS.gene22129	MS.gene072000	PPI
MS.gene22129	MS.gene60525	PPI
MS.gene25353	MS.gene22129	PPI
MS.gene34704	MS.gene22129	PPI
MS.gene22129	MS.gene20329	PPI
MS.gene032861	MS.gene22129	PPI
MS.gene22129	MS.gene033695	PPI
MS.gene22129	MS.gene045451	PPI
MS.gene045439	MS.gene22129	PPI
MS.gene25415	MS.gene22129	PPI
MS.gene22129	MS.gene34802	PPI
MS.gene22129	MS.gene041325	PPI
MS.gene22129	MS.gene95902	PPI
MS.gene22129	MS.gene031575	PPI
MS.gene22129	MS.gene54959	PPI
MS.gene36066	MS.gene22129	PPI
MS.gene22129	MS.gene34706	PPI
MS.gene22129	MS.gene045439	PPI
MS.gene22129	MS.gene66626	PPI
MS.gene041326	MS.gene22129	PPI
MS.gene22129	MS.gene34766	PPI
MS.gene22129	MS.gene34216	PPI
MS.gene045446	MS.gene22129	PPI
MS.gene22129	MS.gene29498	PPI
MS.gene75902	MS.gene22129	PPI
MS.gene22129	MS.gene29873	PPI
MS.gene006727	MS.gene22129	PPI
MS.gene22129	MS.gene045449	PPI
MS.gene63094	MS.gene22129	PPI
MS.gene22129	MS.gene50153	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22129.t1	MTR_3g448340	77.273	462	46	2	1	403	1	462	0.0	707
MS.gene22129.t1	MTR_3g049530	40.890	472	156	7	1	403	1	418	2.12e-93	287
MS.gene22129.t1	MTR_5g080150	37.867	375	196	8	6	348	5	374	1.12e-66	217
MS.gene22129.t1	MTR_1g090907	34.573	457	229	12	4	399	5	452	4.12e-66	217
MS.gene22129.t1	MTR_2g048140	40.717	307	168	7	4	303	5	304	7.83e-63	209
MS.gene22129.t1	MTR_1g090903	32.857	420	242	12	4	399	5	408	4.99e-55	187
MS.gene22129.t1	MTR_3g052500	78.261	92	20	0	306	397	51	142	5.95e-42	144
MS.gene22129.t1	MTR_3g052500	89.130	46	5	0	1	46	1	46	6.32e-19	83.2
MS.gene22129.t1	MTR_0049s0110	23.278	421	222	16	6	367	8	386	3.00e-14	74.3
MS.gene22129.t1	MTR_5g066900	53.333	75	24	3	19	92	19	83	6.66e-14	67.4
MS.gene22129.t1	MTR_1g041245	22.372	371	258	10	6	370	11	357	2.39e-13	71.2
MS.gene22129.t1	MTR_8g066650	27.187	320	188	12	2	303	76	368	3.17e-13	71.2
MS.gene22129.t1	MTR_0049s0110	27.027	259	125	10	6	241	8	225	5.14e-13	69.3
MS.gene22129.t1	MTR_0049s0110	26.923	260	126	10	6	242	8	226	1.44e-12	68.6
MS.gene22129.t1	MTR_4g088845	23.975	317	209	13	6	314	19	311	1.79e-12	68.9
MS.gene22129.t1	MTR_0049s0110	26.923	260	126	10	6	242	8	226	1.95e-12	68.2
MS.gene22129.t1	MTR_2g047858	23.105	277	167	8	6	274	115	353	3.71e-12	68.2
MS.gene22129.t1	MTR_5g080110	57.143	56	24	0	1	56	1	56	5.35e-12	61.2
MS.gene22129.t1	MTR_4g080590	23.866	419	240	15	6	367	23	419	8.03e-12	67.0
MS.gene22129.t1	MTR_5g080160	56.452	62	26	1	1	61	1	62	8.75e-12	63.5
MS.gene22129.t1	MTR_1g021825	25.000	284	151	7	6	276	88	322	1.37e-11	66.2
MS.gene22129.t1	MTR_7g088260	32.000	200	109	7	6	199	91	269	1.79e-11	65.9
MS.gene22129.t1	MTR_1g021825	25.000	284	151	7	6	276	17	251	1.90e-11	65.9
MS.gene22129.t1	MTR_5g080710	22.819	447	264	16	3	396	30	448	2.51e-11	65.5
MS.gene22129.t1	MTR_8g014160	22.837	416	269	14	6	401	41	424	2.66e-11	65.5
MS.gene22129.t1	MTR_2g083840	27.129	317	182	18	4	297	19	309	2.76e-11	65.5
MS.gene22129.t1	MTR_1g071060	43.478	92	43	3	38	123	7	95	3.18e-11	60.1
MS.gene22129.t1	MTR_1g021820	22.776	281	148	7	6	270	28	255	4.98e-11	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22129.t1	AT3G29830	29.493	434	222	16	6	367	8	429	4.85e-39	145
MS.gene22129.t1	AT3G29830	29.425	435	222	16	6	367	8	430	5.02e-39	145
MS.gene22129.t1	AT5G54820	27.586	464	246	16	6	390	7	459	5.36e-37	140
MS.gene22129.t1	AT1G56400	30.721	319	192	11	6	306	13	320	1.40e-35	136
MS.gene22129.t1	AT3G62230	28.505	428	230	15	6	367	8	425	4.03e-35	135
MS.gene22129.t1	AT1G56400	24.841	471	229	15	6	400	13	434	2.36e-28	115
MS.gene22129.t1	AT1G61330	26.349	315	204	10	3	310	7	300	4.80e-17	83.2
MS.gene22129.t1	AT4G14103	28.571	259	160	8	1	250	3	245	5.35e-15	76.3
MS.gene22129.t1	AT4G14103	28.571	259	160	8	1	250	3	245	5.35e-15	76.3
MS.gene22129.t1	AT4G14103	28.571	259	160	8	1	250	3	245	7.17e-15	76.3
MS.gene22129.t1	AT5G22670	28.349	321	197	11	6	311	11	313	1.22e-14	75.9
MS.gene22129.t1	AT5G03100	26.214	309	186	10	6	303	9	286	1.32e-14	74.3
MS.gene22129.t1	AT5G03100	26.214	309	186	10	6	303	9	286	1.32e-14	74.3
MS.gene22129.t1	AT3G58920	28.253	269	143	11	6	246	2	248	1.52e-14	75.5
MS.gene22129.t1	AT3G59210	29.259	270	162	9	6	262	7	260	2.20e-14	75.1
MS.gene22129.t1	AT3G59210	29.259	270	162	9	6	262	7	260	2.20e-14	75.1
MS.gene22129.t1	AT3G59210	29.259	270	162	9	6	262	7	260	2.20e-14	75.1
MS.gene22129.t1	AT3G59210	29.259	270	162	9	6	262	7	260	2.20e-14	75.1
MS.gene22129.t1	AT3G59210	29.259	270	162	9	6	262	7	260	2.20e-14	75.1
MS.gene22129.t1	AT5G56370	27.236	246	160	9	5	248	1	229	1.04e-13	72.0
MS.gene22129.t1	AT3G50710	28.431	306	180	15	5	297	1	280	1.27e-13	72.4
MS.gene22129.t1	AT5G02700	24.671	304	198	11	6	303	27	305	1.34e-13	72.4
MS.gene22129.t1	AT4G14096	28.413	271	168	11	6	266	8	262	1.39e-13	72.4
MS.gene22129.t1	AT4G14096	28.413	271	168	11	6	266	8	262	1.39e-13	72.4
MS.gene22129.t1	AT4G13965	27.138	269	169	8	4	266	8	255	1.41e-13	72.4
MS.gene22129.t1	AT5G56370	27.236	246	160	9	5	248	1	229	1.52e-13	72.4
MS.gene22129.t1	AT5G56370	27.236	246	160	9	5	248	1	229	1.52e-13	72.4
MS.gene22129.t1	AT5G22610	29.482	251	130	12	6	246	18	231	1.94e-13	72.0
MS.gene22129.t1	AT5G22730	28.295	258	156	8	6	250	28	269	5.39e-13	70.5
MS.gene22129.t1	AT5G22730	28.295	258	156	8	6	250	28	269	5.76e-13	70.5
MS.gene22129.t1	AT5G22730	28.295	258	156	8	6	250	28	269	5.76e-13	70.5
MS.gene22129.t1	AT4G10400	29.043	303	177	13	5	298	1	274	7.74e-13	70.1
MS.gene22129.t1	AT4G10400	29.043	303	177	13	5	298	1	274	7.74e-13	70.1
MS.gene22129.t1	AT4G10400	29.043	303	177	13	5	298	1	274	7.74e-13	70.1
MS.gene22129.t1	AT2G42730	26.449	276	162	13	6	262	5	258	8.28e-13	70.1
MS.gene22129.t1	AT4G15060	28.571	252	155	11	3	246	23	257	9.23e-13	69.7
MS.gene22129.t1	AT4G15060	28.571	252	155	11	3	246	169	403	9.64e-13	70.1
MS.gene22129.t1	AT4G00315	27.027	296	169	14	5	274	1	275	1.57e-12	69.3
MS.gene22129.t1	AT3G28410	24.632	272	160	10	6	266	28	265	2.24e-12	68.9
MS.gene22129.t1	AT5G22610	27.941	272	137	12	6	246	18	261	2.65e-12	68.6
MS.gene22129.t1	AT3G59240	26.641	259	151	11	6	247	8	244	4.11e-12	68.2
MS.gene22129.t1	AT3G51530	27.106	273	162	10	6	266	30	277	4.90e-12	67.8
MS.gene22129.t1	AT1G55030	26.042	288	185	10	6	286	9	275	5.36e-12	67.4
MS.gene22129.t1	AT4G09920	30.808	198	113	8	5	196	1	180	5.68e-12	66.6
MS.gene22129.t1	AT4G09920	30.808	198	113	8	5	196	1	180	5.68e-12	66.6
MS.gene22129.t1	AT4G09920	30.808	198	113	8	5	196	1	180	5.68e-12	66.6
MS.gene22129.t1	AT5G56440	30.242	248	141	12	5	241	1	227	5.79e-12	67.4
MS.gene22129.t1	AT1G55030	26.042	288	185	10	6	286	19	285	5.93e-12	67.4
MS.gene22129.t1	AT4G09920	31.156	199	113	8	6	198	2	182	8.03e-12	66.2
MS.gene22129.t1	AT4G09920	31.156	199	113	8	6	198	2	182	8.03e-12	66.2
MS.gene22129.t1	AT4G26340	24.262	305	188	12	5	297	1	274	8.17e-12	67.0
MS.gene22129.t1	AT5G02910	26.370	292	143	10	4	269	9	254	1.64e-11	65.5
MS.gene22129.t1	AT1G58310	26.481	287	172	9	2	262	4	277	1.66e-11	66.2
MS.gene22129.t1	AT5G56690	28.115	313	164	15	8	296	4	279	1.77e-11	65.9
MS.gene22129.t1	AT5G02910	26.370	292	143	10	4	269	9	254	2.26e-11	65.5
MS.gene22129.t1	AT1G66290	22.004	459	258	18	6	400	29	451	2.40e-11	65.5
MS.gene22129.t1	AT5G02910	26.370	292	143	10	4	269	9	254	2.42e-11	65.5
MS.gene22129.t1	AT5G41840	25.000	340	199	14	6	303	14	339	2.65e-11	65.9
MS.gene22129.t1	AT3G58940	34.146	164	83	7	5	156	1	151	5.54e-11	64.7
MS.gene22129.t1	AT3G58940	34.146	164	83	7	5	156	1	151	5.54e-11	64.7
MS.gene22129.t1	AT1G48400	28.696	230	137	7	6	224	10	223	7.09e-11	64.3
MS.gene22129.t1	AT3G59170	26.241	282	153	9	6	246	7	274	7.62e-11	63.9
MS.gene22129.t1	AT3G59190	26.909	275	165	8	6	262	12	268	8.18e-11	63.5
MS.gene22129.t1	AT3G49150	28.185	259	147	14	6	247	16	252	8.31e-11	63.9
MS.gene22129.t1	AT1G69630	23.762	303	195	12	2	288	14	296	8.70e-11	63.9
MS.gene22129.t1	AT5G27750	31.100	209	120	9	4	202	3	197	9.21e-11	63.5
MS.gene22129.t1	AT3G59190	26.909	275	165	8	6	262	12	268	9.42e-11	63.9

Find 45 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGAACTTGGTTAGAAAA+TGG	0.280099	3.4:-42815490	MS.gene22129:CDS
GCACTCAAAGAAGTTTCTTT+AGG	0.291247	3.4:-42816744	MS.gene22129:CDS
AGTTAAGAAAATCCCATCTT+TGG	0.295374	3.4:+42817069	None:intergenic
CCAAACGTTGGTTGCATTTG+TGG	0.319157	3.4:-42817145	MS.gene22129:CDS
TCTAAATCATTTGAAGCCTT+TGG	0.325552	3.4:+42815268	None:intergenic
AACATATTTGAAATTTCTAA+TGG	0.339355	3.4:+42816398	None:intergenic
TAGAAATTTCAAATATGTTA+AGG	0.355350	3.4:-42816394	MS.gene22129:CDS
AACTTGAAATAAGTTAAATT+AGG	0.355765	3.4:+42816534	None:intergenic
CCACAAATGCAACCAACGTT+TGG	0.356961	3.4:+42817145	None:intergenic
GTAGAGATGAATAACTTTAC+TGG	0.357314	3.4:-42815430	MS.gene22129:CDS
TAAATAAAGACATCTTCTTC+AGG	0.367375	3.4:+42816426	None:intergenic
CATCTCACTCATTCCGTTCA+AGG	0.379406	3.4:-42817192	MS.gene22129:CDS
TATTCATTACACAAGGTTCC+TGG	0.397382	3.4:-42815547	MS.gene22129:intron
TCAAAGAAGTTTCTTTAGGT+TGG	0.399769	3.4:-42816740	MS.gene22129:CDS
ATTCATCTCTACCACCTTCA+AGG	0.416614	3.4:+42815441	None:intergenic
ATATCCTCTTCAGTCCAATA+AGG	0.419269	3.4:+42816882	None:intergenic
GAAGAGGTTGATCTATACTT+TGG	0.431197	3.4:-42816465	MS.gene22129:CDS
AGAAGTTTCTTTAGGTTGGA+TGG	0.443378	3.4:-42816736	MS.gene22129:CDS
TTGATGAATTACGTTTGAAA+AGG	0.452536	3.4:-42816608	MS.gene22129:CDS
TTGGATCTGAATTCCAAAGA+TGG	0.478824	3.4:-42817082	MS.gene22129:CDS
TCAACAAGGGGTTAAAGACT+TGG	0.480893	3.4:-42816922	MS.gene22129:CDS
TCTTTGACATTAGCAGATCC+TGG	0.486546	3.4:-42816993	MS.gene22129:CDS
ATGCTTAACATCAACCTTGA+AGG	0.489127	3.4:-42815455	MS.gene22129:CDS
AAGAAACTTCTTTGAGTGCA+TGG	0.490837	3.4:+42816748	None:intergenic
TGGATCTGAATTCCAAAGAT+GGG	0.516644	3.4:-42817081	MS.gene22129:CDS
CAACCAACGTTTGGAAAGGA+TGG	0.516999	3.4:+42817154	None:intergenic
AAATGAAGAGACTGAGGTGG+TGG	0.520516	3.4:-42815329	MS.gene22129:CDS
GTTCTTACAGCTTCCTTGAA+CGG	0.522889	3.4:+42817179	None:intergenic
ATATTCCTCACACTTGGAAG+AGG	0.533412	3.4:-42816481	MS.gene22129:CDS
CATCCATCCTTTCCAAACGT+TGG	0.533511	3.4:-42817157	MS.gene22129:CDS
ATGATGAAAAGCAATGAGTC+TGG	0.543580	3.4:+42817215	None:intergenic
TAGTCCTTATTGGACTGAAG+AGG	0.558479	3.4:-42816886	MS.gene22129:CDS
TATAGAATATTCCTCACACT+TGG	0.559545	3.4:-42816487	MS.gene22129:CDS
TGGAATAGACTTGATTGTGT+TGG	0.566450	3.4:-42816633	MS.gene22129:CDS
GTGTTCATTGAACTCAATGT+TGG	0.575355	3.4:+42817112	None:intergenic
TGTCTGCGGTTTCATTCTCA+AGG	0.580031	3.4:-42816364	MS.gene22129:intron
TCTTTGTAGTAGTATTCACC+AGG	0.596130	3.4:+42815529	None:intergenic
AATGCAACCAACGTTTGGAA+AGG	0.599586	3.4:+42817150	None:intergenic
GTTAAGGTTCTCACTGTCTG+CGG	0.610322	3.4:-42816378	MS.gene22129:CDS
ATCAACCTCTTCCAAGTGTG+AGG	0.617993	3.4:+42816476	None:intergenic
CTTAACATCAACCTTGAAGG+TGG	0.621558	3.4:-42815452	MS.gene22129:CDS
ACTTATGATGACAACACCAA+AGG	0.647451	3.4:-42815284	MS.gene22129:CDS
TCAGAACTAGTAACATGACC+AGG	0.650516	3.4:+42816975	None:intergenic
TGTACAAAATGAAGAGACTG+AGG	0.665014	3.4:-42815335	MS.gene22129:CDS
ACAAAATGAAGAGACTGAGG+TGG	0.771289	3.4:-42815332	MS.gene22129:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACTTGAAATAAGTTAAATT+AGG	+	chr3.4:42815966-42815985	None:intergenic	15.0%
!!	TAGAAATTTCAAATATGTTA+AGG	-	chr3.4:42816103-42816122	MS.gene22129:intron	15.0%
!!!	AAAGATAATTTTTCAACAAT+GGG	+	chr3.4:42815489-42815508	None:intergenic	15.0%
!!!	AACATATTTGAAATTTCTAA+TGG	+	chr3.4:42816102-42816121	None:intergenic	15.0%
!!!	ATTTATTATTTCTTATGCAA+TGG	-	chr3.4:42816567-42816586	MS.gene22129:CDS	15.0%
!!!	GTTATAGATAAATCAAATTT+TGG	+	chr3.4:42816470-42816489	None:intergenic	15.0%
!!!	TAACTTATTTCAAGTTTTAT+GGG	-	chr3.4:42815970-42815989	MS.gene22129:intron	15.0%
!!!	TTAACTTATTTCAAGTTTTA+TGG	-	chr3.4:42815969-42815988	MS.gene22129:intron	15.0%
!!	ATACATTATTCCTAAAATTC+TGG	-	chr3.4:42816838-42816857	MS.gene22129:CDS	20.0%
!!	TACATTATTCCTAAAATTCT+GGG	-	chr3.4:42816839-42816858	MS.gene22129:CDS	20.0%
!!	TGATATAATGTGTACTTATA+TGG	-	chr3.4:42816924-42816943	MS.gene22129:CDS	20.0%
!!!	CAAAGATAATTTTTCAACAA+TGG	+	chr3.4:42815490-42815509	None:intergenic	20.0%
!!!	TCAAATGTTTTGTCATATTT+TGG	+	chr3.4:42816301-42816320	None:intergenic	20.0%
!!!	TTTTAAGCATGAAAAAATGT+TGG	-	chr3.4:42815838-42815857	MS.gene22129:intron	20.0%
!!!	TTTTTCTAGAAATGACTATT+TGG	-	chr3.4:42816895-42816914	MS.gene22129:CDS	20.0%
!!!	TTTTTTTAGAGAATCATGTA+AGG	+	chr3.4:42816504-42816523	None:intergenic	20.0%
!	AATTAAAAACACTTACCTTG+AGG	-	chr3.4:42816320-42816339	MS.gene22129:intron	25.0%
!	TAAATAAAGACATCTTCTTC+AGG	+	chr3.4:42816074-42816093	None:intergenic	25.0%
!	TAGAAAATAATAACTCCTCA+AGG	+	chr3.4:42816338-42816357	None:intergenic	25.0%
!	TTGATGAATTACGTTTGAAA+AGG	-	chr3.4:42815889-42815908	MS.gene22129:intron	25.0%
!!	AATACAACTTGAGAGATTTT+AGG	+	chr3.4:42815695-42815714	None:intergenic	25.0%
!!	ATACAACTTGAGAGATTTTA+GGG	+	chr3.4:42815694-42815713	None:intergenic	25.0%
!!!	ATTTTGATCATAAGAGAACT+TGG	-	chr3.4:42816996-42817015	MS.gene22129:CDS	25.0%
!!!	TAGATTTTTCTAGTCCTTAT+TGG	-	chr3.4:42815601-42815620	MS.gene22129:intron	25.0%
!!!	TGTTGATAGTTGCTTTTTTA+TGG	-	chr3.4:42815917-42815936	MS.gene22129:intron	25.0%
!!!	TTCAATGAACACTTTTTTGT+TGG	-	chr3.4:42815396-42815415	MS.gene22129:CDS	25.0%
!!!	TTGATTTTGCTATTCAACAA+GGG	-	chr3.4:42815562-42815581	MS.gene22129:intron	25.0%
	AAGAGAACTTGGTTAGAAAA+TGG	-	chr3.4:42817007-42817026	MS.gene22129:CDS	30.0%
	AATCATGTATCTCACTTTGT+TGG	-	chr3.4:42816422-42816441	MS.gene22129:CDS	30.0%
	AGTTAAGAAAATCCCATCTT+TGG	+	chr3.4:42815431-42815450	None:intergenic	30.0%
	ATGGTTGTATTCATTACACA+AGG	-	chr3.4:42816943-42816962	MS.gene22129:CDS	30.0%
	ATTTATTGTAGGAAATGGAG+AGG	-	chr3.4:42816598-42816617	MS.gene22129:CDS	30.0%
	CTTTGTTCTCAGTTCAAAAA+AGG	+	chr3.4:42815655-42815674	None:intergenic	30.0%
	GTAGAGATGAATAACTTTAC+TGG	-	chr3.4:42817067-42817086	MS.gene22129:CDS	30.0%
	TATAGAATATTCCTCACACT+TGG	-	chr3.4:42816010-42816029	MS.gene22129:intron	30.0%
	TCAAAGAAGTTTCTTTAGGT+TGG	-	chr3.4:42815757-42815776	MS.gene22129:intron	30.0%
	TCTCCATTTCCTACAATAAA+TGG	+	chr3.4:42816599-42816618	None:intergenic	30.0%
!	TCTAAATCATTTGAAGCCTT+TGG	+	chr3.4:42817232-42817251	None:intergenic	30.0%
!	TCTAATTGTCCCAGAATTTT+AGG	+	chr3.4:42816851-42816870	None:intergenic	30.0%
!	TTCGTTTTCTTATCTGCAAT+GGG	-	chr3.4:42817113-42817132	MS.gene22129:CDS	30.0%
!	TTGCCATTTATTGTAGGAAA+TGG	-	chr3.4:42816593-42816612	MS.gene22129:CDS	30.0%
!	TTTCAACAATGGGATTTTCT+TGG	+	chr3.4:42815479-42815498	None:intergenic	30.0%
!!!	CAATGGGACTGTTTTAAAAA+GGG	-	chr3.4:42817129-42817148	MS.gene22129:CDS	30.0%
!!!	CTTTTTTGAACTGAGAACAA+AGG	-	chr3.4:42815653-42815672	MS.gene22129:intron	30.0%
!!!	GATAGTTGCTTTTTTATGGA+GGG	-	chr3.4:42815921-42815940	MS.gene22129:intron	30.0%
!!!	GTTGATTTTGCTATTCAACA+AGG	-	chr3.4:42815561-42815580	MS.gene22129:intron	30.0%
!!!	TGATAGTTGCTTTTTTATGG+AGG	-	chr3.4:42815920-42815939	MS.gene22129:intron	30.0%
!!!	TGATTTTGCTATTCAACAAG+GGG	-	chr3.4:42815563-42815582	MS.gene22129:intron	30.0%
	AAGAAACTTCTTTGAGTGCA+TGG	+	chr3.4:42815752-42815771	None:intergenic	35.0%
	ACTTATGATGACAACACCAA+AGG	-	chr3.4:42817213-42817232	MS.gene22129:CDS	35.0%
	ATATCCTCTTCAGTCCAATA+AGG	+	chr3.4:42815618-42815637	None:intergenic	35.0%
	ATGATGAAAAGCAATGAGTC+TGG	+	chr3.4:42815285-42815304	None:intergenic	35.0%
	ATGCTTAACATCAACCTTGA+AGG	-	chr3.4:42817042-42817061	MS.gene22129:CDS	35.0%
	GCACTCAAAGAAGTTTCTTT+AGG	-	chr3.4:42815753-42815772	MS.gene22129:intron	35.0%
	GCATGAAAAAATGTTGGACA+TGG	-	chr3.4:42815844-42815863	MS.gene22129:intron	35.0%
	GTGTTCATTGAACTCAATGT+TGG	+	chr3.4:42815388-42815407	None:intergenic	35.0%
	TATTCATTACACAAGGTTCC+TGG	-	chr3.4:42816950-42816969	MS.gene22129:CDS	35.0%
	TCTTTGTAGTAGTATTCACC+AGG	+	chr3.4:42816971-42816990	None:intergenic	35.0%
	TGGAATAGACTTGATTGTGT+TGG	-	chr3.4:42815864-42815883	MS.gene22129:intron	35.0%
	TGGATCTGAATTCCAAAGAT+GGG	-	chr3.4:42815416-42815435	MS.gene22129:CDS	35.0%
	TGTACAAAATGAAGAGACTG+AGG	-	chr3.4:42817162-42817181	MS.gene22129:CDS	35.0%
	TGTATCTCACTTTGTTGGAT+TGG	-	chr3.4:42816427-42816446	MS.gene22129:CDS	35.0%
	TTGCAAAGTGATGAATGTCT+TGG	-	chr3.4:42816687-42816706	MS.gene22129:CDS	35.0%
	TTGGATCTGAATTCCAAAGA+TGG	-	chr3.4:42815415-42815434	MS.gene22129:CDS	35.0%
!	AACTTCTTCCACTCATTTTC+AGG	+	chr3.4:42815729-42815748	None:intergenic	35.0%
!	ATTGTATGCATTGATGAGCT+CGG	-	chr3.4:42816257-42816276	MS.gene22129:intron	35.0%
!	CTTCAGGATTTTCGTATTCA+AGG	+	chr3.4:42816058-42816077	None:intergenic	35.0%
!	CTTCGTTTTCTTATCTGCAA+TGG	-	chr3.4:42817112-42817131	MS.gene22129:CDS	35.0%
!	GAAGAGGTTGATCTATACTT+TGG	-	chr3.4:42816032-42816051	MS.gene22129:intron	35.0%
!	TGGACATTGCCATTTATTGT+AGG	-	chr3.4:42816587-42816606	MS.gene22129:CDS	35.0%
!	TTGTTTTCACTTGCACTTCT+AGG	+	chr3.4:42816219-42816238	None:intergenic	35.0%
!!	AGAAGTTTCTTTAGGTTGGA+TGG	-	chr3.4:42815761-42815780	MS.gene22129:intron	35.0%
!!!	CTGGGACAATTAGAGTTTTT+TGG	-	chr3.4:42816857-42816876	MS.gene22129:CDS	35.0%
!!!	GCAATGGGACTGTTTTAAAA+AGG	-	chr3.4:42817128-42817147	MS.gene22129:CDS	35.0%
!!!	TCTCAACTCGTTTTGAAATG+AGG	-	chr3.4:42816622-42816641	MS.gene22129:CDS	35.0%
	AATGCAACCAACGTTTGGAA+AGG	+	chr3.4:42815350-42815369	None:intergenic	40.0%
	ACAAAATGAAGAGACTGAGG+TGG	-	chr3.4:42817165-42817184	MS.gene22129:CDS	40.0%
	ATATTCCTCACACTTGGAAG+AGG	-	chr3.4:42816016-42816035	MS.gene22129:intron	40.0%
	ATTCATCTCTACCACCTTCA+AGG	+	chr3.4:42817059-42817078	None:intergenic	40.0%
	CTCAGTTCAAAAAAGGCTTC+AGG	+	chr3.4:42815648-42815667	None:intergenic	40.0%
	CTTAACATCAACCTTGAAGG+TGG	-	chr3.4:42817045-42817064	MS.gene22129:CDS	40.0%
	GTTCTTACAGCTTCCTTGAA+CGG	+	chr3.4:42815321-42815340	None:intergenic	40.0%
	TAGTCCTTATTGGACTGAAG+AGG	-	chr3.4:42815611-42815630	MS.gene22129:intron	40.0%
	TCAACAAGGGGTTAAAGACT+TGG	-	chr3.4:42815575-42815594	MS.gene22129:intron	40.0%
	TCAGAACTAGTAACATGACC+AGG	+	chr3.4:42815525-42815544	None:intergenic	40.0%
	TCTTTGACATTAGCAGATCC+TGG	-	chr3.4:42815504-42815523	MS.gene22129:CDS	40.0%
!	GCAGTTTTCCTGAAAATGAG+TGG	-	chr3.4:42815718-42815737	MS.gene22129:intron	40.0%
!!!	AGTTGCTTTTTTATGGAGGG+TGG	-	chr3.4:42815924-42815943	MS.gene22129:intron	40.0%
	AAATGAAGAGACTGAGGTGG+TGG	-	chr3.4:42817168-42817187	MS.gene22129:CDS	45.0%
	ATCAACCTCTTCCAAGTGTG+AGG	+	chr3.4:42816024-42816043	None:intergenic	45.0%
	CAACCAACGTTTGGAAAGGA+TGG	+	chr3.4:42815346-42815365	None:intergenic	45.0%
	CATCCATCCTTTCCAAACGT+TGG	-	chr3.4:42815340-42815359	MS.gene22129:CDS	45.0%
	CATCTCACTCATTCCGTTCA+AGG	-	chr3.4:42815305-42815324	MS.gene22129:CDS	45.0%
	CCACAAATGCAACCAACGTT+TGG	+	chr3.4:42815355-42815374	None:intergenic	45.0%
	GTTAAGGTTCTCACTGTCTG+CGG	-	chr3.4:42816119-42816138	MS.gene22129:intron	45.0%
	TCTTCCTGCTCCTAAGTCAA+AGG	+	chr3.4:42816757-42816776	None:intergenic	45.0%
	TGTCTGCGGTTTCATTCTCA+AGG	-	chr3.4:42816133-42816152	MS.gene22129:intron	45.0%
!	CCAAACGTTGGTTGCATTTG+TGG	-	chr3.4:42815352-42815371	MS.gene22129:CDS	45.0%
	AAGGTGAGGAGCTCCAACAT+AGG	+	chr3.4:42816738-42816757	None:intergenic	50.0%
	AGGTGAGGAGCTCCAACATA+GGG	+	chr3.4:42816737-42816756	None:intergenic	50.0%
	CCAACATAGGGCAGCTATTG+AGG	+	chr3.4:42816725-42816744	None:intergenic	50.0%
	CCTCAATAGCTGCCCTATGT+TGG	-	chr3.4:42816722-42816741	MS.gene22129:CDS	50.0%
	CTGCTCCTAAGTCAAAGGTG+AGG	+	chr3.4:42816752-42816771	None:intergenic	50.0%
!	CTCACCTTTGACTTAGGAGC+AGG	-	chr3.4:42816750-42816769	MS.gene22129:CDS	50.0%
!	GAGCTCCTCACCTTTGACTT+AGG	-	chr3.4:42816744-42816763	MS.gene22129:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	42815249	42817270	42815249	ID=MS.gene22129
chr3.4	mRNA	42815249	42817270	42815249	ID=MS.gene22129.t1;Parent=MS.gene22129
chr3.4	exon	42816365	42817270	42816365	ID=MS.gene22129.t1.exon1;Parent=MS.gene22129.t1
chr3.4	CDS	42816365	42817270	42816365	ID=cds.MS.gene22129.t1;Parent=MS.gene22129.t1
chr3.4	exon	42815249	42815554	42815249	ID=MS.gene22129.t1.exon2;Parent=MS.gene22129.t1
chr3.4	CDS	42815249	42815554	42815249	ID=cds.MS.gene22129.t1;Parent=MS.gene22129.t1

Gene Sequence

>MS.gene22129

ATGGCACAAAACTTTGACATGATCAGTTTGTTACCAGACTCATTGCTTTTCATCATCATCTCACTCATTCCGTTCAAGGAAGCTGTAAGAACATCCATCCTTTCCAAACGTTGGTTGCATTTGTGGAAAAACACAACCAACATTGAGTTCAATGAACACTTTTTTGTTGGATCTGAATTCCAAAGATGGGATTTTCTTAACTTCATTAAAATATGTATTGAAAATCGCCAAGAAAATCCCATTGTTGAAAAATTATCTTTGACATTAGCAGATCCTGGTCATGTTACTAGTTCTGAGATTGTCGAAACATGTGTTGATTTTGCTATTCAACAAGGGGTTAAAGACTTGGATCTAGATTTTTCTAGTCCTTATTGGACTGAAGAGGATATTGAAGAACCTGAAGCCTTTTTTGAACTGAGAACAAAGGTATATGAAAATAAAACCCTAAAATCTCTCAAGTTGTATTCTTGCAGTTTTCCTGAAAATGAGTGGAAGAAGTTCCATGCACTCAAAGAAGTTTCTTTAGGTTGGATGGAATTGAAAAACAATGCAATAAAAACTCTTTTATCGAATTGTAAAATGATCGAGATTTTAAGCATGAAAAAATGTTGGACATGGAATAGACTTGATTGTGTTGGAGTTGATGAATTACGTTTGAAAAGGCTCATTGTTGATAGTTGCTTTTTTATGGAGGGTGGATTTTTAATCGATGCGCCTAATTTAACTTATTTCAAGTTTTATGGGAATATCATGTATTTTGATATAGAATATTCCTCACACTTGGAAGAGGTTGATCTATACTTTGGCCTTGAATACGAAAATCCTGAAGAAGATGTCTTTATTTACAGCGCCATTAGAAATTTCAAATATGTTAAGGTTCTCACTGTCTGCGGTTTCATTCTCAAGGTAATACAAATTTCTGTTTTCTTTTCTGTATGAATTTTGTTCTAGAGTTTTAGATATATATCCTAGAAGTGCAAGTGAAAACAATTTTTTTTTTGTTACTACATTGTATGCATTGATGAGCTCGGCTAAATGCAATTTTTGTACCAAAATATGACAAAACATTTGAATTAAAAACACTTACCTTGAGGAGTTATTATTTTCTAAACAATTGATACTTATATATTATTCATTTCTATTGAGTAAATTTATTCAACATACGCGTTTATTAATCATGTATCTCACTTTGTTGGATTGGATAATGCGAGATTTCTTCCAAAATTTGATTTATCTATAACTGTTGTTATATCCTTACATGATTCTCTAAAAAAATATAAATTAAATGAAGCAAACATCTCAATTAAATGCATTAAACATTTATTATTTCTTATGCAATGGACATTGCCATTTATTGTAGGAAATGGAGAGGATCTCAACTCGTTTTGAAATGAGGCATTTGAAAATGAATACGAGACATTTGAAAATGATGACAAACTTGCAAAGTGATGAATGTCTTGGAGTTAGATTCTTCCTCAATAGCTGCCCTATGTTGGAGCTCCTCACCTTTGACTTAGGAGCAGGAAGATTAATTCATGTAAGTAGAATTTTTATATCTTTATCACGTGTGTTATTCAGTATCAGAAAAGATACATTATTCCTAAAATTCTGGGACAATTAGAGTTTTTTGGTTTTAAATTCAGTTTTTTTTCTAGAAATGACTATTTGGTCTTATTGATATAATGTGTACTTATATGGTTGTATTCATTACACAAGGTTCCTGGTGAATACTACTACAAAGAAGTGAATTTTGATCATAAGAGAACTTGGTTAGAAAATGGAAATAGTTGTCAATGCTTAACATCAACCTTGAAGGTGGTAGAGATGAATAACTTTACTGGAGCTGTGAATGAGATTTTAATGCTTCGTTTTCTTATCTGCAATGGGACTGTTTTAAAAAGGGTTAATATCAATGTACAAAATGAAGAGACTGAGGTGGTGGAAAAATGTCGTGAAGTAGAAGAACTTATGATGACAACACCAAAGGCTTCAAATGATTTAGAGATATTGTTCTGTTATTGA

Protein sequence

>MS.gene22129.t1

MAQNFDMISLLPDSLLFIIISLIPFKEAVRTSILSKRWLHLWKNTTNIEFNEHFFVGSEFQRWDFLNFIKICIENRQENPIVEKLSLTLADPGHVTSSEIVETCVDFAIQQGVKDLDLDFSSPYWTEEDIEEPEAFFELRTKVYENKTLKSLKLYSCSFPENEWKKFHALKEVSLGWMELKNNAIKTLLSNCKMIEILSMKKCWTWNRLDCVGVDELRLKRLIVDSCFFMEGGFLIDAPNLTYFKFYGNIMYFDIEYSSHLEEVDLYFGLEYENPEEDVFIYSAIRNFKYVKVLTVCGFILKVPGEYYYKEVNFDHKRTWLENGNSCQCLTSTLKVVEMNNFTGAVNEILMLRFLICNGTVLKRVNINVQNEETEVVEKCREVEELMMTTPKASNDLEILFCY