AlfalfaGEDB | MS.gene29863

M. sativa cultivar XinJiangDaYe / MS.gene29863

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08387.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29863.t1	XP_024634531.1	86.6	403	53	1	1	402	1	403	1.40E-198	702.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29863.t1	Q9LJ48	28.1	434	227	15	6	366	8	429	1.1e-34	149.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29863.t1	A0A072UVY3	75.5	462	53	2	1	402	1	462	1.3e-190	675.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene071257	MS.gene29863	PPI
MS.gene29863	MS.gene007876	PPI
MS.gene29863	MS.gene033695	PPI
MS.gene29863	MS.gene045451	PPI
MS.gene29863	MS.gene005798	PPI
MS.gene54960	MS.gene29863	PPI
MS.gene63177	MS.gene29863	PPI
MS.gene29863	MS.gene34802	PPI
MS.gene25449	MS.gene29863	PPI
MS.gene033694	MS.gene29863	PPI
MS.gene29863	MS.gene25348	PPI
MS.gene29863	MS.gene031575	PPI
MS.gene29863	MS.gene54959	PPI
MS.gene29863	MS.gene34706	PPI
MS.gene34801	MS.gene29863	PPI
MS.gene75904	MS.gene29863	PPI
MS.gene061531	MS.gene29863	PPI
MS.gene29863	MS.gene66626	PPI
MS.gene29863	MS.gene25450	PPI
MS.gene63113	MS.gene29863	PPI
MS.gene34707	MS.gene29863	PPI
MS.gene29863	MS.gene29498	PPI
MS.gene29863	MS.gene045449	PPI
MS.gene29863	MS.gene50153	PPI
MS.gene29863	MS.gene64988	PPI
MS.gene34804	MS.gene29863	PPI
MS.gene29863	MS.gene035630	PPI
MS.gene29863	MS.gene031577	PPI
MS.gene29863	MS.gene069763	PPI
MS.gene73831	MS.gene29863	PPI
MS.gene29863	MS.gene97999	PPI
MS.gene25347	MS.gene29863	PPI
MS.gene29863	MS.gene053311	PPI
MS.gene29863	MS.gene25299	PPI
MS.gene63213	MS.gene29863	PPI
MS.gene34824	MS.gene29863	PPI
MS.gene64993	MS.gene29863	PPI
MS.gene20329	MS.gene29863	PPI
MS.gene29863	MS.gene005799	PPI
MS.gene29863	MS.gene25298	PPI
MS.gene29863	MS.gene25448	PPI
MS.gene29863	MS.gene006736	PPI
MS.gene25353	MS.gene29863	PPI
MS.gene34704	MS.gene29863	PPI
MS.gene29863	MS.gene033899	PPI
MS.gene032861	MS.gene29863	PPI
MS.gene028696	MS.gene29863	PPI
MS.gene045439	MS.gene29863	PPI
MS.gene25415	MS.gene29863	PPI
MS.gene36066	MS.gene29863	PPI
MS.gene29863	MS.gene045452	PPI
MS.gene29863	MS.gene031570	PPI
MS.gene041326	MS.gene29863	PPI
MS.gene29863	MS.gene34703	PPI
MS.gene69561	MS.gene29863	PPI
MS.gene29863	MS.gene041323	PPI
MS.gene045446	MS.gene29863	PPI
MS.gene29863	MS.gene25386	PPI
MS.gene29863	MS.gene63221	PPI
MS.gene75902	MS.gene29863	PPI
MS.gene22129	MS.gene29863	PPI
MS.gene006727	MS.gene29863	PPI
MS.gene63094	MS.gene29863	PPI
MS.gene006725	MS.gene29863	PPI
MS.gene29873	MS.gene29863	PPI
MS.gene63112	MS.gene29863	PPI
MS.gene29863	MS.gene51793	PPI
MS.gene29863	MS.gene60523	PPI
MS.gene29863	MS.gene055877	PPI
MS.gene91884	MS.gene29863	PPI
MS.gene29863	MS.gene63222	PPI
MS.gene29863	MS.gene25352	PPI
MS.gene031572	MS.gene29863	PPI
MS.gene29863	MS.gene63260	PPI
MS.gene29863	MS.gene032860	PPI
MS.gene29863	MS.gene69561	PPI
MS.gene29863	MS.gene045448	PPI
MS.gene045441	MS.gene29863	PPI
MS.gene29863	MS.gene34825	PPI
MS.gene91885	MS.gene29863	PPI
MS.gene29863	MS.gene032853	PPI
MS.gene041325	MS.gene29863	PPI
MS.gene29863	MS.gene003456	PPI
MS.gene29863	MS.gene062537	PPI
MS.gene29863	MS.gene99980	PPI
MS.gene006692	MS.gene29863	PPI
MS.gene29863	MS.gene25297	PPI
MS.gene01653	MS.gene29863	PPI
MS.gene96120	MS.gene29863	PPI
MS.gene053312	MS.gene29863	PPI
MS.gene29863	MS.gene52136	PPI
MS.gene25344	MS.gene29863	PPI
MS.gene29863	MS.gene09996	PPI
MS.gene29863	MS.gene63256	PPI
MS.gene29863	MS.gene34740	PPI
MS.gene94457	MS.gene29863	PPI
MS.gene20330	MS.gene29863	PPI
MS.gene29863	MS.gene032295	PPI
MS.gene34216	MS.gene29863	PPI
MS.gene29863	MS.gene061286	PPI
MS.gene049943	MS.gene29863	PPI
MS.gene006730	MS.gene29863	PPI
MS.gene29863	MS.gene024262	PPI
MS.gene29863	MS.gene98001	PPI
MS.gene29863	MS.gene94460	PPI
MS.gene72958	MS.gene29863	PPI
MS.gene055882	MS.gene29863	PPI
MS.gene047136	MS.gene29863	PPI
MS.gene63111	MS.gene29863	PPI
MS.gene29863	MS.gene64992	PPI
MS.gene91886	MS.gene29863	PPI
MS.gene069762	MS.gene29863	PPI
MS.gene032853	MS.gene29863	PPI
MS.gene29863	MS.gene031603	PPI
MS.gene29863	MS.gene75903	PPI
MS.gene78164	MS.gene29863	PPI
MS.gene045440	MS.gene29863	PPI
MS.gene98001	MS.gene29863	PPI
MS.gene29863	MS.gene045772	PPI
MS.gene29863	MS.gene045440	PPI
MS.gene29863	MS.gene031573	PPI
MS.gene29863	MS.gene006723	PPI
MS.gene006736	MS.gene29863	PPI
MS.gene96357	MS.gene29863	PPI
MS.gene29863	MS.gene028696	PPI
MS.gene047137	MS.gene29863	PPI
MS.gene29863	MS.gene25451	PPI
MS.gene94458	MS.gene29863	PPI
MS.gene29863	MS.gene95902	PPI
MS.gene045437	MS.gene29863	PPI
MS.gene73780	MS.gene29863	PPI
MS.gene79176	MS.gene29863	PPI
MS.gene29863	MS.gene51795	PPI
MS.gene21278	MS.gene29863	PPI
MS.gene29863	MS.gene34766	PPI
MS.gene72954	MS.gene29863	PPI
MS.gene021295	MS.gene29863	PPI
MS.gene99982	MS.gene29863	PPI
MS.gene29863	MS.gene074346	PPI
MS.gene23706	MS.gene29863	PPI
MS.gene072003	MS.gene29863	PPI
MS.gene29863	MS.gene003455	PPI
MS.gene032852	MS.gene29863	PPI
MS.gene29863	MS.gene010060	PPI
MS.gene072000	MS.gene29863	PPI
MS.gene29863	MS.gene002112	PPI
MS.gene29863	MS.gene040413	PPI
MS.gene29863	MS.gene09997	PPI
MS.gene29863	MS.gene63105	PPI
MS.gene29863	MS.gene006728	PPI
MS.gene64989	MS.gene29863	PPI
MS.gene065373	MS.gene29863	PPI
MS.gene63257	MS.gene29863	PPI
MS.gene055883	MS.gene29863	PPI
MS.gene033897	MS.gene29863	PPI
MS.gene032862	MS.gene29863	PPI
MS.gene29863	MS.gene63259	PPI
MS.gene29863	MS.gene60525	PPI
MS.gene34738	MS.gene29863	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29863.t1	MTR_3g448340	77.056	462	46	3	1	402	1	462	0.0	694
MS.gene29863.t1	MTR_3g049530	40.890	472	155	7	1	402	1	418	1.05e-94	290
MS.gene29863.t1	MTR_5g080150	39.200	375	190	9	6	347	5	374	8.06e-70	225
MS.gene29863.t1	MTR_2g048140	40.717	307	167	8	4	302	5	304	1.00e-62	208
MS.gene29863.t1	MTR_1g090907	39.414	307	171	8	4	302	5	304	9.01e-59	198
MS.gene29863.t1	MTR_1g090903	32.653	392	227	10	31	398	30	408	1.94e-53	183
MS.gene29863.t1	MTR_3g052500	80.435	92	18	0	305	396	51	142	2.42e-42	145
MS.gene29863.t1	MTR_3g052500	86.957	46	6	0	1	46	1	46	1.95e-18	81.6
MS.gene29863.t1	MTR_4g088845	24.127	315	210	11	6	313	19	311	1.40e-14	75.5
MS.gene29863.t1	MTR_5g066900	53.333	75	24	3	19	92	19	83	6.70e-14	67.4
MS.gene29863.t1	MTR_4g080590	23.501	417	243	13	6	366	23	419	1.78e-13	72.0
MS.gene29863.t1	MTR_8g014160	24.286	420	257	16	6	400	41	424	4.69e-13	70.9
MS.gene29863.t1	MTR_8g066650	26.875	320	188	13	2	302	76	368	2.44e-12	68.6
MS.gene29863.t1	MTR_4g098590	23.360	381	271	8	11	383	4	371	2.91e-12	68.2
MS.gene29863.t1	MTR_7g088260	24.878	410	241	18	6	393	91	455	3.13e-12	68.2
MS.gene29863.t1	MTR_1g041245	22.111	398	270	15	6	393	11	378	1.36e-11	65.9
MS.gene29863.t1	MTR_5g080110	55.357	56	25	0	1	56	1	56	1.38e-11	60.1
MS.gene29863.t1	MTR_1g021820	22.823	333	188	10	6	315	28	314	2.08e-11	65.5
MS.gene29863.t1	MTR_5g080160	54.839	62	27	1	1	61	1	62	2.29e-11	62.4
MS.gene29863.t1	MTR_0049s0110	22.637	455	247	16	6	400	8	417	3.62e-11	64.7
MS.gene29863.t1	MTR_0543s0010	24.524	420	236	18	6	400	86	449	3.66e-11	65.1
MS.gene29863.t1	MTR_2g047858	21.352	281	175	7	6	278	115	357	4.00e-11	65.1
MS.gene29863.t1	MTR_1g021825	24.823	282	148	7	6	273	88	319	5.75e-11	64.3
MS.gene29863.t1	MTR_1g021825	25.175	286	142	9	6	273	17	248	5.87e-11	64.3
MS.gene29863.t1	MTR_0049s0110	25.097	259	130	9	6	241	8	225	7.14e-11	62.8
MS.gene29863.t1	MTR_2g083840	26.380	326	190	18	4	303	19	320	7.52e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29863.t1	AT3G29830	28.111	434	227	14	6	366	8	429	2.80e-38	144
MS.gene29863.t1	AT3G29830	28.046	435	227	14	6	366	8	430	4.77e-38	143
MS.gene29863.t1	AT3G62230	28.704	432	223	15	6	366	8	425	6.91e-37	140
MS.gene29863.t1	AT1G56400	26.261	476	256	16	6	399	13	475	2.99e-35	135
MS.gene29863.t1	AT5G54820	30.000	320	194	11	6	305	7	316	3.15e-33	130
MS.gene29863.t1	AT1G56400	25.265	471	226	16	6	399	13	434	6.26e-27	112
MS.gene29863.t1	AT1G61330	25.478	314	207	8	3	309	7	300	7.39e-17	82.4
MS.gene29863.t1	AT5G56370	30.151	199	123	7	5	202	1	184	6.96e-15	75.5
MS.gene29863.t1	AT5G56370	28.049	246	158	9	5	248	1	229	1.10e-14	75.9
MS.gene29863.t1	AT5G56370	28.049	246	158	9	5	248	1	229	1.10e-14	75.9
MS.gene29863.t1	AT5G03100	25.649	308	188	9	6	302	9	286	1.58e-14	74.3
MS.gene29863.t1	AT5G03100	25.649	308	188	9	6	302	9	286	1.58e-14	74.3
MS.gene29863.t1	AT4G26340	21.798	445	272	13	5	400	1	418	1.92e-14	75.1
MS.gene29863.t1	AT5G22670	25.714	420	237	16	6	368	11	412	2.53e-14	74.7
MS.gene29863.t1	AT5G22730	23.810	462	263	17	6	401	28	466	3.85e-14	74.3
MS.gene29863.t1	AT5G22730	23.810	462	263	17	6	401	28	466	3.85e-14	74.3
MS.gene29863.t1	AT4G13965	26.493	268	172	7	4	266	8	255	7.79e-14	73.2
MS.gene29863.t1	AT4G14103	28.571	259	160	8	1	250	3	245	1.00e-13	72.4
MS.gene29863.t1	AT4G14103	28.571	259	160	8	1	250	3	245	1.00e-13	72.4
MS.gene29863.t1	AT4G14103	28.571	259	160	8	1	250	3	245	1.65e-13	72.4
MS.gene29863.t1	AT3G50710	25.503	447	261	20	5	400	1	426	1.70e-13	72.0
MS.gene29863.t1	AT1G55030	26.866	268	168	10	6	266	19	265	1.81e-13	72.0
MS.gene29863.t1	AT1G55030	26.866	268	168	10	6	266	9	255	1.99e-13	72.0
MS.gene29863.t1	AT4G14096	28.413	271	168	11	6	266	8	262	2.73e-13	71.6
MS.gene29863.t1	AT4G14096	28.413	271	168	11	6	266	8	262	2.73e-13	71.6
MS.gene29863.t1	AT3G58920	27.138	269	146	11	6	246	2	248	3.69e-13	71.2
MS.gene29863.t1	AT3G51530	26.838	272	164	9	6	266	30	277	3.99e-13	71.2
MS.gene29863.t1	AT5G22610	29.644	253	127	12	6	246	18	231	6.29e-13	70.5
MS.gene29863.t1	AT4G15060	27.953	254	154	10	3	246	23	257	6.45e-13	70.5
MS.gene29863.t1	AT5G27750	31.884	207	121	9	4	202	3	197	6.55e-13	70.5
MS.gene29863.t1	AT4G10400	28.713	303	182	13	5	298	1	278	6.60e-13	70.1
MS.gene29863.t1	AT4G10400	28.713	303	182	13	5	298	1	278	6.60e-13	70.1
MS.gene29863.t1	AT4G10400	28.713	303	182	13	5	298	1	278	6.60e-13	70.1
MS.gene29863.t1	AT5G22730	27.907	258	157	8	6	250	28	269	6.77e-13	70.1
MS.gene29863.t1	AT5G27750	32.536	209	117	10	4	202	3	197	6.90e-13	70.1
MS.gene29863.t1	AT4G15060	28.400	250	158	9	3	246	169	403	7.32e-13	70.5
MS.gene29863.t1	AT5G02700	23.175	315	210	10	6	313	27	316	1.48e-12	69.3
MS.gene29863.t1	AT4G00315	28.175	252	151	11	5	245	1	233	1.80e-12	68.9
MS.gene29863.t1	AT3G59240	28.016	257	150	11	6	247	8	244	1.88e-12	69.3
MS.gene29863.t1	AT4G09920	31.818	198	111	8	5	196	1	180	2.16e-12	67.8
MS.gene29863.t1	AT4G09920	31.818	198	111	8	5	196	1	180	2.16e-12	67.8
MS.gene29863.t1	AT4G09920	31.818	198	111	8	5	196	1	180	2.16e-12	67.8
MS.gene29863.t1	AT1G66290	23.094	459	252	20	6	399	29	451	2.18e-12	68.9
MS.gene29863.t1	AT3G59210	28.148	270	165	9	6	262	7	260	2.26e-12	68.9
MS.gene29863.t1	AT3G59210	28.148	270	165	9	6	262	7	260	2.26e-12	68.9
MS.gene29863.t1	AT3G59210	28.148	270	165	9	6	262	7	260	2.26e-12	68.9
MS.gene29863.t1	AT3G59210	28.148	270	165	9	6	262	7	260	2.26e-12	68.9
MS.gene29863.t1	AT3G59210	28.148	270	165	9	6	262	7	260	2.26e-12	68.9
MS.gene29863.t1	AT2G42730	26.449	276	162	13	6	262	5	258	2.62e-12	68.6
MS.gene29863.t1	AT1G13780	25.940	266	166	8	4	254	8	257	2.83e-12	67.8
MS.gene29863.t1	AT4G09920	31.980	197	110	8	6	196	2	180	3.49e-12	67.4
MS.gene29863.t1	AT4G09920	31.980	197	110	8	6	196	2	180	3.49e-12	67.4
MS.gene29863.t1	AT2G04230	27.170	265	166	10	2	256	9	256	3.63e-12	68.2
MS.gene29863.t1	AT1G13780	25.940	266	166	8	4	254	8	257	6.33e-12	67.4
MS.gene29863.t1	AT3G26922	28.346	254	151	11	4	248	12	243	7.19e-12	66.2
MS.gene29863.t1	AT3G26922	28.346	254	151	11	4	248	12	243	7.19e-12	66.2
MS.gene29863.t1	AT5G22610	28.788	264	145	10	6	246	18	261	1.01e-11	67.0
MS.gene29863.t1	AT1G69630	25.000	256	161	9	2	246	14	249	1.66e-11	66.2
MS.gene29863.t1	AT5G56420	26.217	267	164	11	1	254	1	247	2.47e-11	65.5
MS.gene29863.t1	AT5G56420	26.217	267	164	11	1	254	1	247	2.47e-11	65.5
MS.gene29863.t1	AT5G56420	26.217	267	164	11	1	254	1	247	2.47e-11	65.5
MS.gene29863.t1	AT5G56440	29.960	247	143	12	5	241	1	227	3.88e-11	64.7
MS.gene29863.t1	AT3G49150	27.668	253	156	11	6	247	16	252	6.85e-11	64.3
MS.gene29863.t1	AT3G58860	26.255	259	157	8	2	247	3	240	8.84e-11	63.9

Find 51 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAACAAGGGGTTAAAGATT+TGG	0.262324	3.3:-38764739	MS.gene29863:CDS
AAGAGAACTTGGTTAGAAAA+TGG	0.280099	3.3:-38763344	MS.gene29863:CDS
CTCAATTCAAACAAGGCTTC+AGG	0.311013	3.3:+38764669	None:intergenic
GCACTCAAAGAAGTTTCTTT+AGG	0.314630	3.3:-38764561	MS.gene29863:CDS
AACTTCATCCACTCATTTCC+AGG	0.345445	3.3:+38764588	None:intergenic
GTAGAGATGAATAACTTTAC+TGG	0.357314	3.3:-38763284	MS.gene29863:CDS
TAGAGATTTCAAACATGTTA+AGG	0.361678	3.3:-38764214	MS.gene29863:CDS
AACATGTTTGAAATCTCTAA+TGG	0.370836	3.3:+38764218	None:intergenic
GTCCTTACAGCTTCCTTGAA+TGG	0.372838	3.3:+38764996	None:intergenic
CCACAAGTGCAACCAACGTT+TGG	0.379296	3.3:+38764962	None:intergenic
TATTCATTACACAAGGTTCC+TGG	0.386852	3.3:-38763401	MS.gene29863:intron
TCAAAGAAGTTTCTTTAGGT+TGG	0.399769	3.3:-38764557	MS.gene29863:CDS
CGTCTTCACATTCGTATTCA+AGG	0.401053	3.3:+38764259	None:intergenic
CATCTCACTCATTCCATTCA+AGG	0.412644	3.3:-38765009	MS.gene29863:CDS
ATTCATCTCTACCACCTTCA+AGG	0.416614	3.3:+38763295	None:intergenic
ATATCCTCTTCAGTCCAATA+AGG	0.419269	3.3:+38764699	None:intergenic
CCAAACGTTGGTTGCACTTG+TGG	0.424960	3.3:-38764962	MS.gene29863:CDS
GAAGAGGTTGATCTATACTT+TGG	0.431197	3.3:-38764282	MS.gene29863:CDS
GTAACTAGTTCTGAGATTAT+CGG	0.442861	3.3:-38764783	MS.gene29863:CDS
AGAAGTTTCTTTAGGTTGGA+TGG	0.443378	3.3:-38764553	MS.gene29863:CDS
AACTTGAAATAAGTTAAGTT+AGG	0.457223	3.3:+38764351	None:intergenic
TTGGATCTGAATTTCAAAGA+TGG	0.458335	3.3:-38764899	MS.gene29863:CDS
TCTAAATCATTTGAAGCCCT+TGG	0.464286	3.3:+38763122	None:intergenic
TCTTTGACATTAGCAGATCC+TGG	0.486546	3.3:-38764810	MS.gene29863:CDS
ATGCTTAACATCAACCTTGA+AGG	0.489127	3.3:-38763309	MS.gene29863:CDS
TGGATCTGAATTTCAAAGAT+GGG	0.503165	3.3:-38764898	MS.gene29863:CDS
TTCCATTCAAGGAAGCTGTA+AGG	0.513675	3.3:-38764998	MS.gene29863:CDS
CAACCAACGTTTGGAAAGGA+TGG	0.527471	3.3:+38764971	None:intergenic
ATATTCCTCACACTTGGAAG+AGG	0.533412	3.3:-38764298	MS.gene29863:CDS
CATCCATCCTTTCCAAACGT+TGG	0.533511	3.3:-38764974	MS.gene29863:CDS
CTCATTTCCAGGAAAACTGC+AGG	0.539615	3.3:+38764599	None:intergenic
TAGTCCTTATTGGACTGAAG+AGG	0.558479	3.3:-38764703	MS.gene29863:CDS
TATAGAATATTCCTCACACT+TGG	0.559545	3.3:-38764304	MS.gene29863:CDS
ATGATGAAAAGCAATGCGTC+TGG	0.561643	3.3:+38765032	None:intergenic
AAGAAACTTCTTTGAGTGCG+TGG	0.566425	3.3:+38764565	None:intergenic
TGGAATAGACTTGATTGTGT+TGG	0.566450	3.3:-38764450	MS.gene29863:CDS
GTGTTCATTGAACTCAATGT+TGG	0.575355	3.3:+38764929	None:intergenic
TGAATACTACTACAAAGAAG+TGG	0.591266	3.3:-38763378	MS.gene29863:CDS
ATTTGTTCTCAATTCAAACA+AGG	0.592244	3.3:+38764662	None:intergenic
TCTTTGTAGTAGTATTCACC+AGG	0.596130	3.3:+38763383	None:intergenic
AGTGCAACCAACGTTTGGAA+AGG	0.605533	3.3:+38764967	None:intergenic
TCAGAACTAGTTACGTGACC+AGG	0.606474	3.3:+38764792	None:intergenic
CGTCTGCGGTTTCATACTCA+AGG	0.615847	3.3:-38764184	MS.gene29863:intron
ATCAACCTCTTCCAAGTGTG+AGG	0.617993	3.3:+38764293	None:intergenic
CTTAACATCAACCTTGAAGG+TGG	0.621558	3.3:-38763306	MS.gene29863:CDS
GTTAAGGTTCTCACCGTCTG+CGG	0.624876	3.3:-38764198	MS.gene29863:CDS
CTTGAGTATGAAACCGCAGA+CGG	0.662966	3.3:+38764185	None:intergenic
TGTGCAAAACGAAGAGACTG+AGG	0.673834	3.3:-38763189	MS.gene29863:CDS
ACTTATGATGACAACACCAA+GGG	0.682464	3.3:-38763138	MS.gene29863:CDS
AACTTATGATGACAACACCA+AGG	0.685575	3.3:-38763139	MS.gene29863:CDS
GAATACGAATGTGAAGACGA+TGG	0.737794	3.3:-38764255	MS.gene29863:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTTTATTCATAAAATTTA+GGG	-	chr3.3:38764658-38764677	MS.gene29863:CDS	10.0%
!!	TATCTTTATTCATAAAATTT+AGG	-	chr3.3:38764657-38764676	MS.gene29863:CDS	10.0%
!!	AATTCATATAGAAAAGAAAA+CGG	+	chr3.3:38764022-38764041	None:intergenic	15.0%
!!!	AAAGATAATTTTTCAACAAT+GGG	+	chr3.3:38763343-38763362	None:intergenic	15.0%
!!!	ATTTATTATTTCTTATGCAA+TGG	-	chr3.3:38764420-38764439	MS.gene29863:CDS	15.0%
!!!	GTTATAGATAAATCAAATTT+TGG	+	chr3.3:38764323-38764342	None:intergenic	15.0%
!!!	TAACTTATTTCAAGTTTTAT+GGG	-	chr3.3:38763824-38763843	MS.gene29863:intron	15.0%
!!!	TTAACTTATTTCAAGTTTTA+TGG	-	chr3.3:38763823-38763842	MS.gene29863:intron	15.0%
!!	AACTTGAAATAAGTTAAGTT+AGG	+	chr3.3:38763820-38763839	None:intergenic	20.0%
!!	TATTAAAAACACTTTCTTTG+AGG	-	chr3.3:38764173-38764192	MS.gene29863:intron	20.0%
!!!	CAAAGATAATTTTTCAACAA+TGG	+	chr3.3:38763344-38763363	None:intergenic	20.0%
!!!	TTTTAAGCATGAAAAAATGT+TGG	-	chr3.3:38763692-38763711	MS.gene29863:intron	20.0%
!!!	TTTTTTTAGAGAATCATGTA+AGG	+	chr3.3:38764357-38764376	None:intergenic	20.0%
!	ATTTGTTCTCAATTCAAACA+AGG	+	chr3.3:38763509-38763528	None:intergenic	25.0%
!	TAGAGATTTCAAACATGTTA+AGG	-	chr3.3:38763954-38763973	MS.gene29863:intron	25.0%
!!	AAAACAACTTGAGAGATTTT+AGG	+	chr3.3:38763549-38763568	None:intergenic	25.0%
!!	AAACAACTTGAGAGATTTTA+GGG	+	chr3.3:38763548-38763567	None:intergenic	25.0%
!!	AACATGTTTGAAATCTCTAA+TGG	+	chr3.3:38763953-38763972	None:intergenic	25.0%
!!!	ATTTTGATCATAAGAGAACT+TGG	-	chr3.3:38764813-38764832	MS.gene29863:CDS	25.0%
!!!	TAGATTTTTCTAGTCCTTAT+TGG	-	chr3.3:38763455-38763474	MS.gene29863:intron	25.0%
!!!	TGTTGATAGTTGCTTTTTTA+TGG	-	chr3.3:38763771-38763790	MS.gene29863:intron	25.0%
!!!	TTCAATGAACACTTTTTTGT+TGG	-	chr3.3:38763250-38763269	MS.gene29863:CDS	25.0%
!!!	TTGATTTTGCTATTCAACAA+GGG	-	chr3.3:38763416-38763435	MS.gene29863:intron	25.0%
!!!	TTTTTCTAGCAATGACTATT+TGG	-	chr3.3:38764712-38764731	MS.gene29863:CDS	25.0%
	AAGAGAACTTGGTTAGAAAA+TGG	-	chr3.3:38764824-38764843	MS.gene29863:CDS	30.0%
	AATCATGTATCTCACTTTGT+TGG	-	chr3.3:38764275-38764294	MS.gene29863:CDS	30.0%
	ATGCTTGTATTCATTACACA+AGG	-	chr3.3:38764760-38764779	MS.gene29863:CDS	30.0%
	ATTTATTGTAGGAAATGGAG+AGG	-	chr3.3:38764451-38764470	MS.gene29863:CDS	30.0%
	GTAGAGATGAATAACTTTAC+TGG	-	chr3.3:38764884-38764903	MS.gene29863:CDS	30.0%
	TATAGAATATTCCTCACACT+TGG	-	chr3.3:38763864-38763883	MS.gene29863:intron	30.0%
	TCAAAGAAGTTTCTTTAGGT+TGG	-	chr3.3:38763611-38763630	MS.gene29863:intron	30.0%
	TCTCCATTTCCTACAATAAA+TGG	+	chr3.3:38764452-38764471	None:intergenic	30.0%
	TGAATACTACTACAAAGAAG+TGG	-	chr3.3:38764790-38764809	MS.gene29863:CDS	30.0%
	TGGATCTGAATTTCAAAGAT+GGG	-	chr3.3:38763270-38763289	MS.gene29863:CDS	30.0%
	TTGGATCTGAATTTCAAAGA+TGG	-	chr3.3:38763269-38763288	MS.gene29863:CDS	30.0%
!	TTCGTTTTCTTATCAGCAAT+GGG	-	chr3.3:38764930-38764949	MS.gene29863:CDS	30.0%
!	TTGCCATTTATTGTAGGAAA+TGG	-	chr3.3:38764446-38764465	MS.gene29863:CDS	30.0%
!	TTTCAACAATGGGATTTTCT+TGG	+	chr3.3:38763333-38763352	None:intergenic	30.0%
!!	GTAACTAGTTCTGAGATTAT+CGG	-	chr3.3:38763385-38763404	MS.gene29863:CDS	30.0%
!!!	CAATGGGACTGTTTTAAAAA+GGG	-	chr3.3:38764946-38764965	MS.gene29863:CDS	30.0%
!!!	GATAGTTGCTTTTTTATGGA+GGG	-	chr3.3:38763775-38763794	MS.gene29863:intron	30.0%
!!!	GTTGATTTTGCTATTCAACA+AGG	-	chr3.3:38763415-38763434	MS.gene29863:intron	30.0%
!!!	TAGGGACAATTAGAGTTTTT+TGG	-	chr3.3:38764676-38764695	MS.gene29863:CDS	30.0%
!!!	TGATAGTTGCTTTTTTATGG+AGG	-	chr3.3:38763774-38763793	MS.gene29863:intron	30.0%
!!!	TGATTTTGCTATTCAACAAG+GGG	-	chr3.3:38763417-38763436	MS.gene29863:intron	30.0%
	AACTTATGATGACAACACCA+AGG	-	chr3.3:38765029-38765048	MS.gene29863:CDS	35.0%
	ACTAACGTTTCGTCATATCT+TGG	+	chr3.3:38764154-38764173	None:intergenic	35.0%
	ACTTATGATGACAACACCAA+GGG	-	chr3.3:38765030-38765049	MS.gene29863:CDS	35.0%
	ATATCCTCTTCAGTCCAATA+AGG	+	chr3.3:38763472-38763491	None:intergenic	35.0%
	ATGCTTAACATCAACCTTGA+AGG	-	chr3.3:38764859-38764878	MS.gene29863:CDS	35.0%
	GCACTCAAAGAAGTTTCTTT+AGG	-	chr3.3:38763607-38763626	MS.gene29863:intron	35.0%
	GCATGAAAAAATGTTGGACA+TGG	-	chr3.3:38763698-38763717	MS.gene29863:intron	35.0%
	GTGTTCATTGAACTCAATGT+TGG	+	chr3.3:38763242-38763261	None:intergenic	35.0%
	TATTCATTACACAAGGTTCC+TGG	-	chr3.3:38764767-38764786	MS.gene29863:CDS	35.0%
	TCAACAAGGGGTTAAAGATT+TGG	-	chr3.3:38763429-38763448	MS.gene29863:intron	35.0%
	TCTTTGTAGTAGTATTCACC+AGG	+	chr3.3:38764788-38764807	None:intergenic	35.0%
	TGGAATAGACTTGATTGTGT+TGG	-	chr3.3:38763718-38763737	MS.gene29863:intron	35.0%
	TGTATCTCACTTTGTTGGAT+TGG	-	chr3.3:38764280-38764299	MS.gene29863:CDS	35.0%
	TTGCAAAGTGATGAATGTCT+TGG	-	chr3.3:38764540-38764559	MS.gene29863:CDS	35.0%
!	ATTGTATGCATTGATGAGCT+CGG	-	chr3.3:38764110-38764129	MS.gene29863:intron	35.0%
!	CTTCGTTTTCTTATCAGCAA+TGG	-	chr3.3:38764929-38764948	MS.gene29863:CDS	35.0%
!	GAAGAGGTTGATCTATACTT+TGG	-	chr3.3:38763886-38763905	MS.gene29863:intron	35.0%
!	TCTAAATCATTTGAAGCCCT+TGG	+	chr3.3:38765049-38765068	None:intergenic	35.0%
!	TGGACATTGCCATTTATTGT+AGG	-	chr3.3:38764440-38764459	MS.gene29863:CDS	35.0%
!!	AGAAGTTTCTTTAGGTTGGA+TGG	-	chr3.3:38763615-38763634	MS.gene29863:intron	35.0%
!!!	GCAATGGGACTGTTTTAAAA+AGG	-	chr3.3:38764945-38764964	MS.gene29863:CDS	35.0%
!!!	TCTCAACTCGTTTTGAAATG+AGG	-	chr3.3:38764475-38764494	MS.gene29863:CDS	35.0%
	AACTTCATCCACTCATTTCC+AGG	+	chr3.3:38763583-38763602	None:intergenic	40.0%
	AAGAAACTTCTTTGAGTGCG+TGG	+	chr3.3:38763606-38763625	None:intergenic	40.0%
	ATATTCCTCACACTTGGAAG+AGG	-	chr3.3:38763870-38763889	MS.gene29863:intron	40.0%
	ATGATGAAAAGCAATGCGTC+TGG	+	chr3.3:38763139-38763158	None:intergenic	40.0%
	ATTCATCTCTACCACCTTCA+AGG	+	chr3.3:38764876-38764895	None:intergenic	40.0%
	CATCTCACTCATTCCATTCA+AGG	-	chr3.3:38763159-38763178	MS.gene29863:CDS	40.0%
	CGTCTTCACATTCGTATTCA+AGG	+	chr3.3:38763912-38763931	None:intergenic	40.0%
	CTCAATTCAAACAAGGCTTC+AGG	+	chr3.3:38763502-38763521	None:intergenic	40.0%
	CTTAACATCAACCTTGAAGG+TGG	-	chr3.3:38764862-38764881	MS.gene29863:CDS	40.0%
	GAATACGAATGTGAAGACGA+TGG	-	chr3.3:38763913-38763932	MS.gene29863:intron	40.0%
	TAGTCCTTATTGGACTGAAG+AGG	-	chr3.3:38763465-38763484	MS.gene29863:intron	40.0%
	TCTTCCTGATCCTAAGTCAA+AGG	+	chr3.3:38764610-38764629	None:intergenic	40.0%
	TCTTTGACATTAGCAGATCC+TGG	-	chr3.3:38763358-38763377	MS.gene29863:CDS	40.0%
	TTCCATTCAAGGAAGCTGTA+AGG	-	chr3.3:38763170-38763189	MS.gene29863:CDS	40.0%
!	TTGTTTTCCTGCAGTTTTCC+TGG	-	chr3.3:38763562-38763581	MS.gene29863:intron	40.0%
!!!	AGTTGCTTTTTTATGGAGGG+TGG	-	chr3.3:38763778-38763797	MS.gene29863:intron	40.0%
	AGTGCAACCAACGTTTGGAA+AGG	+	chr3.3:38763204-38763223	None:intergenic	45.0%
	ATCAACCTCTTCCAAGTGTG+AGG	+	chr3.3:38763878-38763897	None:intergenic	45.0%
	CAACCAACGTTTGGAAAGGA+TGG	+	chr3.3:38763200-38763219	None:intergenic	45.0%
	CATCCATCCTTTCCAAACGT+TGG	-	chr3.3:38763194-38763213	MS.gene29863:CDS	45.0%
	CTCATTTCCAGGAAAACTGC+AGG	+	chr3.3:38763572-38763591	None:intergenic	45.0%
	CTGATCCTAAGTCAAAGGTG+AGG	+	chr3.3:38764605-38764624	None:intergenic	45.0%
	CTTGAGTATGAAACCGCAGA+CGG	+	chr3.3:38763986-38764005	None:intergenic	45.0%
	GTCCTTACAGCTTCCTTGAA+TGG	+	chr3.3:38763175-38763194	None:intergenic	45.0%
	TCAGAACTAGTTACGTGACC+AGG	+	chr3.3:38763379-38763398	None:intergenic	45.0%
	TGTGCAAAACGAAGAGACTG+AGG	-	chr3.3:38764979-38764998	MS.gene29863:CDS	45.0%
!	CTCACCTTTGACTTAGGATC+AGG	-	chr3.3:38764603-38764622	MS.gene29863:CDS	45.0%
!	GCAGTTTTCCTGGAAATGAG+TGG	-	chr3.3:38763572-38763591	MS.gene29863:intron	45.0%
	AAGGTGAGGAGCTCCAACAT+AGG	+	chr3.3:38764591-38764610	None:intergenic	50.0%
	AGGTGAGGAGCTCCAACATA+GGG	+	chr3.3:38764590-38764609	None:intergenic	50.0%
	CCAACATAGGGCAGCTATTG+AGG	+	chr3.3:38764578-38764597	None:intergenic	50.0%
	CCACAAGTGCAACCAACGTT+TGG	+	chr3.3:38763209-38763228	None:intergenic	50.0%
	CCTCAATAGCTGCCCTATGT+TGG	-	chr3.3:38764575-38764594	MS.gene29863:CDS	50.0%
	CGTCTGCGGTTTCATACTCA+AGG	-	chr3.3:38763984-38764003	MS.gene29863:intron	50.0%
	GTTAAGGTTCTCACCGTCTG+CGG	-	chr3.3:38763970-38763989	MS.gene29863:intron	50.0%
!	CCAAACGTTGGTTGCACTTG+TGG	-	chr3.3:38763206-38763225	MS.gene29863:CDS	50.0%
!	GAGCTCCTCACCTTTGACTT+AGG	-	chr3.3:38764597-38764616	MS.gene29863:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	38763103	38765087	38763103	ID=MS.gene29863
chr3.3	mRNA	38763103	38765087	38763103	ID=MS.gene29863.t1;Parent=MS.gene29863
chr3.3	exon	38764185	38765087	38764185	ID=MS.gene29863.t1.exon1;Parent=MS.gene29863.t1
chr3.3	CDS	38764185	38765087	38764185	ID=cds.MS.gene29863.t1;Parent=MS.gene29863.t1
chr3.3	exon	38763103	38763408	38763103	ID=MS.gene29863.t1.exon2;Parent=MS.gene29863.t1
chr3.3	CDS	38763103	38763408	38763103	ID=cds.MS.gene29863.t1;Parent=MS.gene29863.t1

Gene Sequence

>MS.gene29863

ATGGCACAAAACTTTGACATGATCAGTTTGTTACCAGACGCATTGCTTTTCATCATCATCTCACTCATTCCATTCAAGGAAGCTGTAAGGACATCCATCCTTTCCAAACGTTGGTTGCACTTGTGGAAAAACACAACCAACATTGAGTTCAATGAACACTTTTTTGTTGGATCTGAATTTCAAAGATGGGATTTTCTTAACTTCATTAAAATATGTATTGAAAATCGCCAAGAAAATCCCATTGTTGAAAAATTATCTTTGACATTAGCAGATCCTGGTCACGTAACTAGTTCTGAGATTATCGGAACATGTGTTGATTTTGCTATTCAACAAGGGGTTAAAGATTTGGATCTAGATTTTTCTAGTCCTTATTGGACTGAAGAGGATATTGAAGAACCTGAAGCCTTGTTTGAATTGAGAACAAATGTATATGAAAATAAAACCCTAAAATCTCTCAAGTTGTTTTCCTGCAGTTTTCCTGGAAATGAGTGGATGAAGTTCCACGCACTCAAAGAAGTTTCTTTAGGTTGGATGGAATTGAAAAACAATGCAATAAAAACTCTTTTATCGAATTGTAAAATGATCGAGATTTTAAGCATGAAAAAATGTTGGACATGGAATAGACTTGATTGTGTTGGAGTTGATGAATTACGTTTGAAAAAGCTCATTGTTGATAGTTGCTTTTTTATGGAGGGTGGATTTTTAATCGATGCGCCTAACTTAACTTATTTCAAGTTTTATGGGAATATCATGTATTTTGATATAGAATATTCCTCACACTTGGAAGAGGTTGATCTATACTTTGGCCTTGAATACGAATGTGAAGACGATGGCTTTTTTTACAGCGCCATTAGAGATTTCAAACATGTTAAGGTTCTCACCGTCTGCGGTTTCATACTCAAGGTAATACAAATTTCCGTTTTCTTTTCTATATGAATTTTTTTCTATAGACTTTTAGATATATATACTAGAAATGCAAGTGAAAACACATTTTCTTTTGTTACTACATTGTATGCATTGATGAGCTCGGCTAAACGCAATTTTTGTACCAAGATATGACGAAACGTTAGTATTAAAAACACTTTCTTTGAGGAGTTATTATTTTCTAAACAATTAATACTTATATATTATTCATTTCTATTGAGTAAATTTATTCAACATATGCGTTTATTAATCATGTATCTCACTTTGTTGGATTGGATAATGCGAGATTTCTTCCAAAATTTGATTTATCTATAACTGTTGTTATATCCTTACATGATTCTCTAAAAAAATATAAATTAAATGAAGCAAACATCTCAATTAAATGCATTAAACATTTATTATTTCTTATGCAATGGACATTGCCATTTATTGTAGGAAATGGAGAGGATCTCAACTCGTTTTGAAATGAGGCATTTGAAAATGAATACGAGACATTTGAAAATGATGACAAACTTGCAAAGTGATGAATGTCTTGGAGTCAGATTCTTCCTCAATAGCTGCCCTATGTTGGAGCTCCTCACCTTTGACTTAGGATCAGGAAGATTAATTCATGTAAGTAGATTTTTTTTATATCTTTATTCATAAAATTTAGGGACAATTAGAGTTTTTTGGTTTTAAATTCAGTTTTTTCTAGCAATGACTATTTGGTCTTATTGATATAAAGTCTACTTATATGCTTGTATTCATTACACAAGGTTCCTGGTGAATACTACTACAAAGAAGTGGATTTTGATCATAAGAGAACTTGGTTAGAAAATGGAAATAGTTGTCAATGCTTAACATCAACCTTGAAGGTGGTAGAGATGAATAACTTTACTGGAGCTGTGAATGAGATTTTAATGCTTCGTTTTCTTATCAGCAATGGGACTGTTTTAAAAAGGGTTAATATCAATGTGCAAAACGAAGAGACTGAGGTAGTAGAAAAATGTCGTAAAGTAGAAGAACTTATGATGACAACACCAAGGGCTTCAAATGATTTAGAGATATTGTTCTGTTATTGA

Protein sequence

>MS.gene29863.t1

MAQNFDMISLLPDALLFIIISLIPFKEAVRTSILSKRWLHLWKNTTNIEFNEHFFVGSEFQRWDFLNFIKICIENRQENPIVEKLSLTLADPGHVTSSEIIGTCVDFAIQQGVKDLDLDFSSPYWTEEDIEEPEALFELRTNVYENKTLKSLKLFSCSFPGNEWMKFHALKEVSLGWMELKNNAIKTLLSNCKMIEILSMKKCWTWNRLDCVGVDELRLKKLIVDSCFFMEGGFLIDAPNLTYFKFYGNIMYFDIEYSSHLEEVDLYFGLEYECEDDGFFYSAIRDFKHVKVLTVCGFILKVPGEYYYKEVDFDHKRTWLENGNSCQCLTSTLKVVEMNNFTGAVNEILMLRFLISNGTVLKRVNINVQNEETEVVEKCRKVEELMMTTPRASNDLEILFCY