AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20330.t1 MTR_1g090907 90.989 455 41 0 1 455 1 455 0.0 848
MS.gene20330.t1 MTR_2g048140 81.798 456 82 1 1 455 1 456 0.0 759
MS.gene20330.t1 MTR_1g090903 61.050 457 130 5 1 455 1 411 0.0 520
MS.gene20330.t1 MTR_3g448340 35.470 468 269 12 5 452 4 458 2.73e-81 259
MS.gene20330.t1 MTR_5g080150 34.988 403 219 8 9 403 7 374 2.16e-65 215
MS.gene20330.t1 MTR_3g049530 30.353 481 236 16 4 453 3 415 9.42e-51 177
MS.gene20330.t1 MTR_1g071060 72.222 90 25 0 39 128 7 96 3.51e-38 134
MS.gene20330.t1 MTR_7g029260 35.714 126 80 1 303 427 34 159 2.07e-17 81.6
MS.gene20330.t1 MTR_2g072720 25.641 468 279 21 2 433 25 459 2.14e-12 69.3
MS.gene20330.t1 MTR_8g468470 21.983 464 309 19 3 436 10 450 2.68e-12 68.9
MS.gene20330.t1 MTR_5g066890 35.955 89 51 3 318 404 1 85 8.47e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20330.t1 AT5G54820 29.977 437 279 10 9 425 9 438 4.19e-59 201
MS.gene20330.t1 AT1G56400 29.362 470 287 12 12 444 18 479 9.47e-59 200
MS.gene20330.t1 AT3G62230 27.293 458 314 12 8 453 9 459 7.05e-52 182
MS.gene20330.t1 AT3G29830 29.954 434 271 13 10 423 11 431 6.41e-51 179
MS.gene20330.t1 AT3G29830 29.466 431 278 12 10 423 11 432 1.99e-49 175
MS.gene20330.t1 AT1G56400 30.250 400 235 11 82 444 46 438 1.34e-48 172
MS.gene20330.t1 AT2G17305 31.707 164 107 3 295 454 106 268 3.69e-19 87.4
MS.gene20330.t1 AT3G58940 26.277 274 152 12 6 252 1 251 8.96e-12 67.8
MS.gene20330.t1 AT3G58940 26.277 274 152 12 6 252 1 251 8.96e-12 67.8
MS.gene20330.t1 AT3G28410 20.972 391 260 12 12 391 33 385 5.65e-11 64.7
MS.gene20330.t1 AT5G56370 23.210 405 255 16 9 402 4 363 8.64e-11 63.9
MS.gene20330.t1 AT5G56370 23.210 405 255 16 9 402 4 363 8.64e-11 63.9

Find 78 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAATACTTCAAATATTC+TGG 0.116368 1.3:-63939735 MS.gene20330:CDS
TTGATTTCTCTGATCCTAAA+TGG 0.158820 1.3:-63940097 MS.gene20330:CDS
AAGTAGACCTTAAGAAATTT+TGG 0.209673 1.3:-63939045 MS.gene20330:CDS
AAATTGTTATCTTCCCATTT+AGG 0.210930 1.3:+63940083 None:intergenic
CGCAAATTCTTTGAACGCTT+TGG 0.247622 1.3:+63938818 None:intergenic
TCTTGAGAATTGTTGGAATT+TGG 0.254022 1.3:-63939850 MS.gene20330:CDS
GAATTTGGCAAACTTTGATT+TGG 0.267542 1.3:-63939835 MS.gene20330:CDS
AAAATTTGTTATCAACTTGT+AGG 0.276512 1.3:-63939890 MS.gene20330:CDS
TGCACTCAAAGATTTGTCTT+TGG 0.298466 1.3:-63939943 MS.gene20330:CDS
GCTCAAAAGCTGCCCATTGC+TGG 0.325341 1.3:-63939257 MS.gene20330:CDS
TATTTGAAGTATTTAAGATT+TGG 0.333013 1.3:+63939741 None:intergenic
ATCAATGTCTACCTCTTCTA+TGG 0.339261 1.3:+63939680 None:intergenic
TAAATGGGAAGATAACAATT+TGG 0.344219 1.3:-63940081 MS.gene20330:CDS
ATATCTTTAACCAGTTGTTT+AGG 0.344304 1.3:+63940357 None:intergenic
ATTTCTTAAGGTCTACTTGA+TGG 0.344830 1.3:+63939050 None:intergenic
GCACTCAAAGATTTGTCTTT+GGG 0.346946 1.3:-63939942 MS.gene20330:CDS
TCTTATGCAAGGAAAAGTTT+TGG 0.354339 1.3:-63938921 MS.gene20330:CDS
GTTTGAGTCTTGAGAATTGT+TGG 0.356911 1.3:-63939857 MS.gene20330:CDS
ATTGCAAGACGAAGTGATTC+TGG 0.358588 1.3:+63940425 None:intergenic
GATAAACCTGCGTTGGCAAT+TGG 0.365643 1.3:+63940039 None:intergenic
AGAGTTCATTGCCACGTTCA+TGG 0.376627 1.3:+63939637 None:intergenic
AGTCATAAGTTCTTCATTTG+TGG 0.376656 1.3:+63938952 None:intergenic
AATTTGGCAAACTTTGATTT+GGG 0.381661 1.3:-63939834 MS.gene20330:CDS
TAAACAACTGGTTAAAGATA+TGG 0.390128 1.3:-63940355 MS.gene20330:CDS
TTAAATACTTCAAATATTCT+GGG 0.391463 1.3:-63939734 MS.gene20330:CDS
TGTTCTCCTTGCGGCTTCTT+CGG 0.394545 1.3:+63940385 None:intergenic
ATCGAAATTGATGTGGTCTT+CGG 0.395752 1.3:+63940328 None:intergenic
TTGAAGGTGGTAGAAGTAAT+CGG 0.408674 1.3:-63938983 MS.gene20330:CDS
GAGTTTGCCAAAATTTCTTA+AGG 0.408773 1.3:+63939038 None:intergenic
TTCAAATCCACAAAGCTCAT+AGG 0.414020 1.3:+63939285 None:intergenic
ATGCATCCCTATGAGCTTTG+TGG 0.415004 1.3:-63939292 MS.gene20330:CDS
ACTGCAAACTGTTAGAATCT+TGG 0.420814 1.3:+63939587 None:intergenic
TTTCTTAAGGTCTACTTGAT+GGG 0.428036 1.3:+63939051 None:intergenic
CTCCTTGCGGCTTCTTCGGA+AGG 0.435231 1.3:+63940389 None:intergenic
TCATCGACTTCATCACAAAC+TGG 0.438278 1.3:-63940247 MS.gene20330:CDS
TTACTTCAGATGTAAATGTA+AGG 0.455810 1.3:-63939704 MS.gene20330:CDS
GAACAAGTATCCTAAACAAC+TGG 0.457299 1.3:-63940367 MS.gene20330:CDS
TGATTTCTCTGATCCTAAAT+GGG 0.458338 1.3:-63940096 MS.gene20330:CDS
TTTCAAACCCTCAAACTTGT+GGG 0.475584 1.3:-63940178 MS.gene20330:CDS
TCAAAGATTTGTCTTTGGGT+TGG 0.475817 1.3:-63939938 MS.gene20330:CDS
TTGTTTCCCCACAAGTTTGA+GGG 0.476639 1.3:+63940171 None:intergenic
AGCACTTGGATTAACAAGAT+TGG 0.479484 1.3:-63939805 MS.gene20330:CDS
TGTTCCATCCGGAGATGAAT+CGG 0.486372 1.3:-63939365 MS.gene20330:CDS
TGGCATGTTATTTCTTGCCA+TGG 0.492796 1.3:+63940463 None:intergenic
ATTAAGTTGTCGAATGAAGA+TGG 0.494144 1.3:-63938887 MS.gene20330:CDS
CATCCGCTCCGATTCATCTC+CGG 0.508668 1.3:+63939357 None:intergenic
AAGTCCACAAGTTATATTTG+AGG 0.508872 1.3:-63939215 MS.gene20330:intron
TCAAATCCACAAAGCTCATA+GGG 0.508994 1.3:+63939286 None:intergenic
ACTTGTTGCTTTCTTATGCA+AGG 0.514283 1.3:-63938932 MS.gene20330:CDS
TTCTTAAGGTCTACTTGATG+GGG 0.518779 1.3:+63939052 None:intergenic
CCAACGCAGGTTTATCAACT+CGG 0.534120 1.3:-63940032 MS.gene20330:CDS
AGTTTGCAGTTATTTACTCC+AGG 0.537677 1.3:-63939574 MS.gene20330:intron
ATTGTTTCCCCACAAGTTTG+AGG 0.540471 1.3:+63940170 None:intergenic
ATTTCAAACCCTCAAACTTG+TGG 0.540788 1.3:-63940179 MS.gene20330:CDS
ACTTGGATTAACAAGATTGG+TGG 0.553442 1.3:-63939802 MS.gene20330:CDS
GTTGTCGAATGAAGATGGTA+AGG 0.554984 1.3:-63938882 MS.gene20330:CDS
TACTTCAGATGTAAATGTAA+GGG 0.555889 1.3:-63939703 MS.gene20330:CDS
TTGCGGCTTCTTCGGAAGGT+AGG 0.559549 1.3:+63940393 None:intergenic
GCAAACTGTTAGAATCTTGG+CGG 0.561620 1.3:+63939590 None:intergenic
TCACTTAAATTGTATTCATG+TGG 0.563141 1.3:-63939996 MS.gene20330:CDS
TAGGATACTTGTTCTCCTTG+CGG 0.568084 1.3:+63940376 None:intergenic
AACTGTTAGAATCTTGGCGG+CGG 0.578443 1.3:+63939593 None:intergenic
GATTTGGGAGATGAAGCACT+TGG 0.579721 1.3:-63939819 MS.gene20330:CDS
CCGAGTTGATAAACCTGCGT+TGG 0.582629 1.3:+63940032 None:intergenic
CTGACCTCAAATATAACTTG+TGG 0.595453 1.3:+63939211 None:intergenic
CTAAAAGTTGTGCACATCCA+CGG 0.603973 1.3:+63938849 None:intergenic
TAAATACTTCAAATATTCTG+GGG 0.604843 1.3:-63939733 MS.gene20330:CDS
CGCTCCGATTCATCTCCGGA+TGG 0.616905 1.3:+63939361 None:intergenic
TACCTTCCGAAGAAGCCGCA+AGG 0.619142 1.3:-63940391 MS.gene20330:CDS
GAGAAAACTTGTCGATGTCA+CGG 0.625302 1.3:+63940207 None:intergenic
TTCAAACCCTCAAACTTGTG+GGG 0.628166 1.3:-63940177 MS.gene20330:CDS
AAATGTAAGGGCCATAGAAG+AGG 0.630077 1.3:-63939691 MS.gene20330:CDS
TCTGGTATCAAAGAAAACAA+TGG 0.640546 1.3:+63940443 None:intergenic
GTTGTTCCAATTGCCAACGC+AGG 0.640937 1.3:-63940045 MS.gene20330:CDS
TGGCGGCGGAGAAATCATGA+AGG 0.685071 1.3:+63939607 None:intergenic
GAATCAGATTTCCATGAACG+TGG 0.696307 1.3:-63939648 MS.gene20330:CDS
GGTAAGGTCGAATTTCGCCG+TGG 0.737861 1.3:-63938866 MS.gene20330:CDS
CATCCGGAGATGAATCGGAG+CGG 0.748262 1.3:-63939360 MS.gene20330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATACTTCAAATATTCT+GGG - chr1.3:63939529-63939548 MS.gene20330:intron 15.0%
!!! TATTTGAAGTATTTAAGATT+TGG + chr1.3:63939525-63939544 None:intergenic 15.0%
!! CTCTAAACATTGTTATAAAA+AGG - chr1.3:63939874-63939893 MS.gene20330:CDS 20.0%
!! CTTAAATACTTCAAATATTC+TGG - chr1.3:63939528-63939547 MS.gene20330:intron 20.0%
!! GTCATTGATATATAGTTATA+TGG - chr1.3:63940129-63940148 MS.gene20330:CDS 20.0%
!! TAAATACTTCAAATATTCTG+GGG - chr1.3:63939530-63939549 MS.gene20330:intron 20.0%
!!! AAAATTTGTTATCAACTTGT+AGG - chr1.3:63939373-63939392 MS.gene20330:intron 20.0%
!!! CTTTTTATAACAATGTTTAG+AGG + chr1.3:63939876-63939895 None:intergenic 20.0%
! AAATTGTTATCTTCCCATTT+AGG + chr1.3:63939183-63939202 None:intergenic 25.0%
! ATATCTTTAACCAGTTGTTT+AGG + chr1.3:63938909-63938928 None:intergenic 25.0%
! ATGCTTGAAAAATACTTTGA+AGG - chr1.3:63940264-63940283 MS.gene20330:CDS 25.0%
! TAAACAACTGGTTAAAGATA+TGG - chr1.3:63938908-63938927 MS.gene20330:CDS 25.0%
! TAAATGGGAAGATAACAATT+TGG - chr1.3:63939182-63939201 MS.gene20330:intron 25.0%
! TACTTCAGATGTAAATGTAA+GGG - chr1.3:63939560-63939579 MS.gene20330:intron 25.0%
! TCACTTAAATTGTATTCATG+TGG - chr1.3:63939267-63939286 MS.gene20330:CDS 25.0%
! TTACTTCAGATGTAAATGTA+AGG - chr1.3:63939559-63939578 MS.gene20330:intron 25.0%
!! AAGTAGACCTTAAGAAATTT+TGG - chr1.3:63940218-63940237 MS.gene20330:CDS 25.0%
!! AATTTGGCAAACTTTGATTT+GGG - chr1.3:63939429-63939448 MS.gene20330:intron 25.0%
!!! AAAGTATTTTTCAAGCATTG+AGG + chr1.3:63940262-63940281 None:intergenic 25.0%
!!! AATTCAATTAAAAGAGTACC+TGG + chr1.3:63939710-63939729 None:intergenic 25.0%
AAGTCCACAAGTTATATTTG+AGG - chr1.3:63940048-63940067 MS.gene20330:CDS 30.0%
AGTCATAAGTTCTTCATTTG+TGG + chr1.3:63940314-63940333 None:intergenic 30.0%
ATTTCTTAAGGTCTACTTGA+TGG + chr1.3:63940216-63940235 None:intergenic 30.0%
GAATTTGGCAAACTTTGATT+TGG - chr1.3:63939428-63939447 MS.gene20330:intron 30.0%
GAGTTTGCCAAAATTTCTTA+AGG + chr1.3:63940228-63940247 None:intergenic 30.0%
TATAAAAAGGTTGTTCCATC+CGG - chr1.3:63939887-63939906 MS.gene20330:CDS 30.0%
TCTGGTATCAAAGAAAACAA+TGG + chr1.3:63938823-63938842 None:intergenic 30.0%
TCTTGAGAATTGTTGGAATT+TGG - chr1.3:63939413-63939432 MS.gene20330:intron 30.0%
TGATTTCTCTGATCCTAAAT+GGG - chr1.3:63939167-63939186 MS.gene20330:intron 30.0%
TGCTTGTGTTTAGAAATTGT+AGG - chr1.3:63939767-63939786 MS.gene20330:CDS 30.0%
TGTTTGCTCGTTTATATTAC+AGG - chr1.3:63940177-63940196 MS.gene20330:CDS 30.0%
TTGATTTCTCTGATCCTAAA+TGG - chr1.3:63939166-63939185 MS.gene20330:intron 30.0%
TTTCTTAAGGTCTACTTGAT+GGG + chr1.3:63940215-63940234 None:intergenic 30.0%
! AGTTTTCATCGAAATTGATG+TGG + chr1.3:63938945-63938964 None:intergenic 30.0%
! ATTAAGTTGTCGAATGAAGA+TGG - chr1.3:63940376-63940395 MS.gene20330:CDS 30.0%
! CTTGAAAAATACTTTGAAGG+TGG - chr1.3:63940267-63940286 MS.gene20330:CDS 30.0%
! TCTTATGCAAGGAAAAGTTT+TGG - chr1.3:63940342-63940361 MS.gene20330:CDS 30.0%
ACTGCAAACTGTTAGAATCT+TGG + chr1.3:63939679-63939698 None:intergenic 35.0%
ACTTGGATTAACAAGATTGG+TGG - chr1.3:63939461-63939480 MS.gene20330:intron 35.0%
AGCACTTGGATTAACAAGAT+TGG - chr1.3:63939458-63939477 MS.gene20330:intron 35.0%
AGTTTGCAGTTATTTACTCC+AGG - chr1.3:63939689-63939708 MS.gene20330:CDS 35.0%
ATCAATGTCTACCTCTTCTA+TGG + chr1.3:63939586-63939605 None:intergenic 35.0%
ATCGAAATTGATGTGGTCTT+CGG + chr1.3:63938938-63938957 None:intergenic 35.0%
ATTGCTGGAAAAACTCACAT+TGG - chr1.3:63940021-63940040 MS.gene20330:CDS 35.0%
ATTTCAAACCCTCAAACTTG+TGG - chr1.3:63939084-63939103 MS.gene20330:intron 35.0%
CTGACCTCAAATATAACTTG+TGG + chr1.3:63940055-63940074 None:intergenic 35.0%
GAACAAGTATCCTAAACAAC+TGG - chr1.3:63938896-63938915 MS.gene20330:CDS 35.0%
GCACTCAAAGATTTGTCTTT+GGG - chr1.3:63939321-63939340 MS.gene20330:CDS 35.0%
GTTTGAGTCTTGAGAATTGT+TGG - chr1.3:63939406-63939425 MS.gene20330:intron 35.0%
TCAAAGATTTGTCTTTGGGT+TGG - chr1.3:63939325-63939344 MS.gene20330:CDS 35.0%
TCAAATCCACAAAGCTCATA+GGG + chr1.3:63939980-63939999 None:intergenic 35.0%
TGCACTCAAAGATTTGTCTT+TGG - chr1.3:63939320-63939339 MS.gene20330:CDS 35.0%
TTCAAATCCACAAAGCTCAT+AGG + chr1.3:63939981-63940000 None:intergenic 35.0%
TTCTTAAGGTCTACTTGATG+GGG + chr1.3:63940214-63940233 None:intergenic 35.0%
TTGAAGGTGGTAGAAGTAAT+CGG - chr1.3:63940280-63940299 MS.gene20330:CDS 35.0%
TTTCAAACCCTCAAACTTGT+GGG - chr1.3:63939085-63939104 MS.gene20330:intron 35.0%
! AATGTGAGTTTTTCCAGCAA+TGG + chr1.3:63940022-63940041 None:intergenic 35.0%
! ATGTGAGTTTTTCCAGCAAT+GGG + chr1.3:63940021-63940040 None:intergenic 35.0%
!! ACTTGTTGCTTTCTTATGCA+AGG - chr1.3:63940331-63940350 MS.gene20330:CDS 35.0%
AAATGTAAGGGCCATAGAAG+AGG - chr1.3:63939572-63939591 MS.gene20330:intron 40.0%
ATTGCAAGACGAAGTGATTC+TGG + chr1.3:63938841-63938860 None:intergenic 40.0%
ATTGTTTCCCCACAAGTTTG+AGG + chr1.3:63939096-63939115 None:intergenic 40.0%
CGCAAATTCTTTGAACGCTT+TGG + chr1.3:63940448-63940467 None:intergenic 40.0%
CTAAAAGTTGTGCACATCCA+CGG + chr1.3:63940417-63940436 None:intergenic 40.0%
GAATCAGATTTCCATGAACG+TGG - chr1.3:63939615-63939634 MS.gene20330:CDS 40.0%
GAGAAAACTTGTCGATGTCA+CGG + chr1.3:63939059-63939078 None:intergenic 40.0%
GCAAACTGTTAGAATCTTGG+CGG + chr1.3:63939676-63939695 None:intergenic 40.0%
GTTGTCGAATGAAGATGGTA+AGG - chr1.3:63940381-63940400 MS.gene20330:CDS 40.0%
TAGGATACTTGTTCTCCTTG+CGG + chr1.3:63938890-63938909 None:intergenic 40.0%
TTCAAACCCTCAAACTTGTG+GGG - chr1.3:63939086-63939105 MS.gene20330:intron 40.0%
TTGTTTCCCCACAAGTTTGA+GGG + chr1.3:63939095-63939114 None:intergenic 40.0%
!! TCATCGACTTCATCACAAAC+TGG - chr1.3:63939016-63939035 MS.gene20330:CDS 40.0%
AACTGTTAGAATCTTGGCGG+CGG + chr1.3:63939673-63939692 None:intergenic 45.0%
AGAGTTCATTGCCACGTTCA+TGG + chr1.3:63939629-63939648 None:intergenic 45.0%
ATGCATCCCTATGAGCTTTG+TGG - chr1.3:63939971-63939990 MS.gene20330:CDS 45.0%
CCAACGCAGGTTTATCAACT+CGG - chr1.3:63939231-63939250 MS.gene20330:CDS 45.0%
TGTTCCATCCGGAGATGAAT+CGG - chr1.3:63939898-63939917 MS.gene20330:CDS 45.0%
! GATAAACCTGCGTTGGCAAT+TGG + chr1.3:63939227-63939246 None:intergenic 45.0%
! GATTTGGGAGATGAAGCACT+TGG - chr1.3:63939444-63939463 MS.gene20330:intron 45.0%
!!! GTTGCTTTTGCGACACAACA+GGG - chr1.3:63939126-63939145 MS.gene20330:intron 45.0%
!!! TGTTGCTTTTGCGACACAAC+AGG - chr1.3:63939125-63939144 MS.gene20330:intron 45.0%
GTTGTTCCAATTGCCAACGC+AGG - chr1.3:63939218-63939237 MS.gene20330:CDS 50.0%
TGTTCTCCTTGCGGCTTCTT+CGG + chr1.3:63938881-63938900 None:intergenic 50.0%
!! CCGAGTTGATAAACCTGCGT+TGG + chr1.3:63939234-63939253 None:intergenic 50.0%
CATCCGCTCCGATTCATCTC+CGG + chr1.3:63939909-63939928 None:intergenic 55.0%
CATCCGGAGATGAATCGGAG+CGG - chr1.3:63939903-63939922 MS.gene20330:CDS 55.0%
GCTCAAAAGCTGCCCATTGC+TGG - chr1.3:63940006-63940025 MS.gene20330:CDS 55.0%
GGTAAGGTCGAATTTCGCCG+TGG - chr1.3:63940397-63940416 MS.gene20330:CDS 55.0%
TACCTTCCGAAGAAGCCGCA+AGG - chr1.3:63938872-63938891 MS.gene20330:CDS 55.0%
TGGCGGCGGAGAAATCATGA+AGG + chr1.3:63939659-63939678 None:intergenic 55.0%
TTGCGGCTTCTTCGGAAGGT+AGG + chr1.3:63938873-63938892 None:intergenic 55.0%
CGCTCCGATTCATCTCCGGA+TGG + chr1.3:63939905-63939924 None:intergenic 60.0%
CTCCTTGCGGCTTCTTCGGA+AGG + chr1.3:63938877-63938896 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 63938802 63940483 63938802 ID=MS.gene20330
chr1.3 mRNA 63938802 63940483 63938802 ID=MS.gene20330.t1;Parent=MS.gene20330
chr1.3 exon 63939575 63940483 63939575 ID=MS.gene20330.t1.exon1;Parent=MS.gene20330.t1
chr1.3 CDS 63939575 63940483 63939575 ID=cds.MS.gene20330.t1;Parent=MS.gene20330.t1
chr1.3 exon 63939216 63939389 63939216 ID=MS.gene20330.t1.exon2;Parent=MS.gene20330.t1
chr1.3 CDS 63939216 63939389 63939216 ID=cds.MS.gene20330.t1;Parent=MS.gene20330.t1
chr1.3 exon 63938802 63939086 63938802 ID=MS.gene20330.t1.exon3;Parent=MS.gene20330.t1
chr1.3 CDS 63938802 63939086 63938802 ID=cds.MS.gene20330.t1;Parent=MS.gene20330.t1
Gene Sequence

>MS.gene20330

ATGGCAAGAAATAACATGCCATTGTTTTCTTTGATACCAGAATCACTTCGTCTTGCAATCGTTTCTTTCCTACCTTCCGAAGAAGCCGCAAGGAGAACAAGTATCCTAAACAACTGGTTAAAGATATGGCAGTCCGAAGACCACATCAATTTCGATGAAAACTTCTTCGTCGATTCAAGTTCTGATGAAATCAAACAAGCGCAGAGAAAAGTTTTCATCGACTTCATCACAAACTGGATTGCGCAGTTCACTAGCCGTGACATCGACAAGTTTTCTCTCACGATTTCAAACCCTCAAACTTGTGGGGAAACAATTGAACGTTGTGTTGCTTTTGCGACACAACAGGGCGTGAAATATTTAACATTTGATTTCTCTGATCCTAAATGGGAAGATAACAATTTGGATGATAAACATGCGTTGTTCCAATTGCCAACGCAGGTTTATCAACTCGGATCATCACTTGAATCACTTAAATTGTATTCATGTGGTTTTGATATGCAAAATCTTCATAACTTTGTTGCACTCAAAGATTTGTCTTTGGGTTGGATTGAAGTTAAAATCAAAACTCTCAAAAATTTGTTATCAACTTGTAGGACGATTCAAAGTTTGAGTCTTGAGAATTGTTGGAATTTGGCAAACTTTGATTTGGGAGATGAAGCACTTGGATTAACAAGATTGGTGGTAAACAATTGTGATTCTGAGTATTTCATATTTAATGCACCAAATCTTAAATACTTCAAATATTCTGGGGCTGTGTTTACTTCAGATGTAAATGTAAGGGCCATAGAAGAGGTAGACATTGATTTTTCTCAAGAATCAGATTTCCATGAACGTGGCAATGAACTCTGCGAAATCCTTCATGATTTCTCCGCCGCCAAGATTCTAACAGTTTGCAGTTATTTACTCCAGGTACTCTTTTAATTGAATTATTGTTAATGATTATGCATGTATTTTAATTAATTTCTTGCTTGTGTTTAGAAATTGTAGGTTACTTTGTGCATACATTTAAATATCTCATATGAATCAACTTTTTGAATTGATCTTTTTTTCTAATTGATGTTTTTATTGTTACCTCTAAACATTGTTATAAAAAGGTTGTTCCATCCGGAGATGAATCGGAGCGGATGCAAAGTGCTTTGAATGTAAAACATTTGATTCTAAAGACTCAAATGCATCCCTATGAGCTTTGTGGATTTGAATTCATGCTCAAAAGCTGCCCATTGCTGGAAAAACTCACATTGGACATAAGTCCACAAGTTATATTTGAGGTCAGTCATAAAATACTTAACATTTCATGTCTCTCTTTTTCTAAATATTTAACATAGTAGTCATTGATATATAGTTATATGGTTTGATCATTTTTAACACTTTTGAATGTTTGCTCGTTTATATTACAGGACTATACATTTCCCCATCAAGTAGACCTTAAGAAATTTTGGCAAACTCGTCGAGTGTTTCCTCAATGCTTGAAAAATACTTTGAAGGTGGTAGAAGTAATCGGATTGAGAGCCACAAATGAAGAACTTATGACTTGTTGCTTTCTTATGCAAGGAAAAGTTTTGGAACAGATTAATATTAAGTTGTCGAATGAAGATGGTAAGGTCGAATTTCGCCGTGGATGTGCACAACTTTTAGTCAATGCTCCAAAGCGTTCAAAGAATTTGCGAATATCAATTGATTGA
Protein sequence

>MS.gene20330.t1

MARNNMPLFSLIPESLRLAIVSFLPSEEAARRTSILNNWLKIWQSEDHINFDENFFVDSSSDEIKQAQRKVFIDFITNWIAQFTSRDIDKFSLTISNPQTCGETIERCVAFATQQGVKYLTFDFSDPKWEDNNLDDKHALFQLPTQVYQLGSSLESLKLYSCGFDMQNLHNFVALKDLSLGWIEVKIKTLKNLLSTCRTIQSLSLENCWNLANFDLGDEALGLTRLVVNNCDSEYFIFNAPNLKYFKYSGAVFTSDVNVRAIEEVDIDFSQESDFHERGNELCEILHDFSAAKILTVCSYLLQVVPSGDESERMQSALNVKHLILKTQMHPYELCGFEFMLKSCPLLEKLTLDISPQVIFEDYTFPHQVDLKKFWQTRRVFPQCLKNTLKVVEVIGLRATNEELMTCCFLMQGKVLEQINIKLSNEDGKVEFRRGCAQLLVNAPKRSKNLRISID