AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067048


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067048.t1 MTR_3g086050 94.570 221 11 1 1 221 1 220 2.70e-136 382
MS.gene067048.t1 MTR_3g052760 42.667 225 101 9 10 221 25 234 2.00e-54 174
MS.gene067048.t1 MTR_3g092460 40.252 159 75 4 64 221 95 234 1.92e-32 118
MS.gene067048.t1 MTR_3g092460 40.385 156 73 4 67 221 129 265 7.07e-31 115
MS.gene067048.t1 MTR_0004s0510 31.395 172 100 4 38 208 41 195 6.79e-21 87.4
MS.gene067048.t1 MTR_0004s0510 34.507 142 75 4 68 208 70 194 3.42e-20 85.5
MS.gene067048.t1 MTR_4g088445 34.783 92 57 2 124 212 38 129 8.51e-13 63.9
MS.gene067048.t1 MTR_7g102590 43.590 78 38 2 132 208 40 112 1.53e-12 63.2
MS.gene067048.t1 MTR_5g081530 29.365 126 72 4 91 207 17 134 5.07e-12 62.0
MS.gene067048.t1 MTR_4g010320 35.398 113 63 2 119 221 118 230 7.17e-12 63.2
MS.gene067048.t1 MTR_6g022000 36.458 96 55 4 119 208 28 123 1.31e-11 60.8
MS.gene067048.t1 MTR_6g061940 28.037 107 72 2 118 221 53 157 8.16e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067048.t1 AT4G27670 42.286 175 72 7 56 221 72 226 5.72e-37 129
MS.gene067048.t1 AT5G51440 33.333 177 97 5 33 208 39 195 8.25e-20 84.7
MS.gene067048.t1 AT1G52560 45.652 92 46 3 122 209 121 212 1.82e-19 84.0
MS.gene067048.t1 AT1G52560 45.652 92 46 3 122 209 128 219 2.27e-19 84.0
MS.gene067048.t1 AT4G25200 34.965 143 76 5 68 208 68 195 4.52e-18 79.7
MS.gene067048.t1 AT1G07400 31.783 129 72 6 91 209 19 141 1.85e-12 63.5
MS.gene067048.t1 AT5G37670 36.792 106 58 4 125 221 28 133 9.19e-12 61.2
MS.gene067048.t1 AT1G53540 32.673 101 65 2 124 221 55 155 2.71e-11 60.5
MS.gene067048.t1 AT1G59860 34.951 103 60 4 117 215 46 145 5.69e-11 59.3
MS.gene067048.t1 AT1G54050 33.333 117 63 4 104 206 22 137 6.90e-11 59.3
MS.gene067048.t1 AT1G54050 33.333 117 63 4 104 206 22 137 6.90e-11 59.3

Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCAAACTGCACATTCTT+TGG 0.245312 3.4:-80292807 None:intergenic
CTGCTGCACCGATAGGGTTA+TGG 0.285020 3.4:+80292439 MS.gene067048:CDS
AAGGAGTAGGTGGGTATAGA+AGG 0.294040 3.4:+80292577 MS.gene067048:CDS
ACAAAGAGGATGTTAAAGTT+TGG 0.296691 3.4:+80292673 MS.gene067048:CDS
TGTGCAGTTTGAGAATATTA+AGG 0.336710 3.4:+80292815 MS.gene067048:CDS
TGCTGCACCGATAGGGTTAT+GGG 0.365142 3.4:+80292440 MS.gene067048:CDS
CAAAGAGGATGTTAAAGTTT+GGG 0.367241 3.4:+80292674 MS.gene067048:CDS
TTCTCTTGCTTCACCTGTCA+TGG 0.368553 3.4:-80292346 None:intergenic
GAATATTAAGGCTGAGGTTA+AGG 0.368713 3.4:+80292827 MS.gene067048:CDS
CACTCTTTATCCTCAAATCT+TGG 0.368943 3.4:+80292261 MS.gene067048:CDS
AACATTAAGATCAAGGACTT+TGG 0.390389 3.4:-80292895 None:intergenic
AGTTTGTCTTGATGAACTCT+TGG 0.419007 3.4:-80292304 None:intergenic
TGATGAACTCTTGGAAATCA+TGG 0.438584 3.4:-80292295 None:intergenic
GTCATTGAACATTAAGATCA+AGG 0.444311 3.4:-80292902 None:intergenic
ATTAAGGCTGAGGTTAAGGA+TGG 0.462230 3.4:+80292831 MS.gene067048:CDS
TCATCGCCGCTACCGATTGA+AGG 0.462817 3.4:+80292558 MS.gene067048:CDS
TCTCCTTCTTTAACCTCCCA+TGG 0.463067 3.4:-80292612 None:intergenic
CTCTCTTCTTGGGTTCGGAC+TGG 0.467954 3.4:-80292410 None:intergenic
TCAATTGTACTCATTGCAAA+CGG 0.479546 3.4:-80292528 None:intergenic
CATAACCCTATCGGTGCAGC+AGG 0.490168 3.4:-80292438 None:intergenic
GAATAAGTAGTAGTAGCCTT+AGG 0.490783 3.4:-80292873 None:intergenic
CAATTGTACTCATTGCAAAC+GGG 0.496144 3.4:-80292527 None:intergenic
AAGGACAGTGCAAGAGATGA+TGG 0.500427 3.4:+80292479 MS.gene067048:CDS
TCAATCGGTAGCGGCGATGA+AGG 0.505690 3.4:-80292555 None:intergenic
AGGAAGCAGCTCTATTATGA+AGG 0.508056 3.4:+80293042 MS.gene067048:CDS
CTTCGAGTCAAAGCCATGAC+AGG 0.510747 3.4:+80292333 MS.gene067048:CDS
GTTTGAGAATATTAAGGCTG+AGG 0.515148 3.4:+80292821 MS.gene067048:CDS
AGAATGATGAAGATGAAGAA+TGG 0.516116 3.4:+80292745 MS.gene067048:CDS
TCTTTACATAACCATTCCTA+AGG 0.516609 3.4:+80292857 MS.gene067048:CDS
AGTAGGTGGGTATAGAAGGA+GGG 0.522121 3.4:+80292581 MS.gene067048:CDS
ACATCCTCTTTGTTCATACC+AGG 0.528873 3.4:-80292663 None:intergenic
GAGTAGGTGGGTATAGAAGG+AGG 0.533601 3.4:+80292580 MS.gene067048:CDS
CTGTCATGGCTTTGACTCGA+AGG 0.534564 3.4:-80292332 None:intergenic
TACCCACCTACTCCTTCAAT+CGG 0.537111 3.4:-80292570 None:intergenic
GATGTTTAAATCTGGTTGTG+AGG 0.537899 3.4:+80293022 MS.gene067048:intron
AACTCTTGGAAATCATGGAC+AGG 0.539369 3.4:-80292290 None:intergenic
TAAGGCTGAGGTTAAGGATG+GGG 0.545488 3.4:+80292833 MS.gene067048:CDS
GTAGGTGGGTATAGAAGGAG+GGG 0.548522 3.4:+80292582 MS.gene067048:CDS
GAGAAAGTGAATACAAGATG+AGG 0.559520 3.4:+80292631 MS.gene067048:CDS
TCTTCTTGGGTTCGGACTGG+TGG 0.560738 3.4:-80292407 None:intergenic
GAAGAATGGTCTAAGAGCTA+TGG 0.561468 3.4:+80292759 MS.gene067048:CDS
AAGATGAGGTTTGATATGCC+TGG 0.574216 3.4:+80292645 MS.gene067048:CDS
ACACCATGGGAGGTTAAAGA+AGG 0.575898 3.4:+80292609 MS.gene067048:CDS
TTGGGTGGAAGAGAAGATGT+TGG 0.576341 3.4:+80292692 MS.gene067048:CDS
GGAAACAATGGAAAGCATGA+TGG 0.576838 3.4:+80292500 MS.gene067048:CDS
CCGCTACCGATTGAAGGAGT+AGG 0.578817 3.4:+80292564 MS.gene067048:CDS
AGAGGATGTTAAAGTTTGGG+TGG 0.581219 3.4:+80292677 MS.gene067048:CDS
GCAAGAGATGATGGAAACAA+TGG 0.589078 3.4:+80292488 MS.gene067048:CDS
CAGGTTAAGACCAAGATTTG+AGG 0.593235 3.4:-80292271 None:intergenic
TTAAGGCTGAGGTTAAGGAT+GGG 0.596993 3.4:+80292832 MS.gene067048:CDS
AATCGATCCCATAACCCTAT+CGG 0.598439 3.4:-80292447 None:intergenic
TATTGTTTGATCTTGAAACA+TGG 0.608054 3.4:-80292377 None:intergenic
GAGGGGAAGAACACCATGGG+AGG 0.617779 3.4:+80292599 MS.gene067048:CDS
AGTAGTAGTAGCCTTAGGAA+TGG 0.626005 3.4:-80292868 None:intergenic
TTGCAATGAGTACAATTGAG+TGG 0.632366 3.4:+80292532 MS.gene067048:CDS
CTACCGATTGAAGGAGTAGG+TGG 0.639864 3.4:+80292567 MS.gene067048:CDS
GGGATCGATTTCTTACGACA+AGG 0.653086 3.4:+80292460 MS.gene067048:CDS
AAGGAGGGGAAGAACACCAT+GGG 0.659587 3.4:+80292596 MS.gene067048:CDS
AATGGTCTAAGAGCTATGGT+AGG 0.662268 3.4:+80292763 MS.gene067048:CDS
CCTACTCCTTCAATCGGTAG+CGG 0.682306 3.4:-80292564 None:intergenic
TACCGATTGAAGGAGTAGGT+GGG 0.683545 3.4:+80292568 MS.gene067048:CDS
GAAGGAGGGGAAGAACACCA+TGG 0.687857 3.4:+80292595 MS.gene067048:CDS
TATGCCTGGTATGAACAAAG+AGG 0.732695 3.4:+80292659 MS.gene067048:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAACATCTTACAAAAA+GGG - chr3.4:80293013-80293032 None:intergenic 15.0%
!! GATTTAAACATCTTACAAAA+AGG - chr3.4:80293014-80293033 None:intergenic 20.0%
!!! TTTTGTAAGATGTTTAAATC+TGG + chr3.4:80293014-80293033 MS.gene067048:intron 20.0%
! TATTGTTTGATCTTGAAACA+TGG - chr3.4:80292380-80292399 None:intergenic 25.0%
!! ATCTTCATCATTCTTCTTTT+TGG - chr3.4:80292739-80292758 None:intergenic 25.0%
!!! AATCATTCATTTTTTTTCCC+TGG + chr3.4:80292925-80292944 MS.gene067048:intron 25.0%
AAAGCTTAATTAACAACTCC+AGG - chr3.4:80292946-80292965 None:intergenic 30.0%
AACATTAAGATCAAGGACTT+TGG - chr3.4:80292898-80292917 None:intergenic 30.0%
AAGCTTAATTAACAACTCCA+GGG - chr3.4:80292945-80292964 None:intergenic 30.0%
ACAAAGAGGATGTTAAAGTT+TGG + chr3.4:80292673-80292692 MS.gene067048:CDS 30.0%
AGAATGATGAAGATGAAGAA+TGG + chr3.4:80292745-80292764 MS.gene067048:CDS 30.0%
CAAAGAGGATGTTAAAGTTT+GGG + chr3.4:80292674-80292693 MS.gene067048:CDS 30.0%
GTCATTGAACATTAAGATCA+AGG - chr3.4:80292905-80292924 None:intergenic 30.0%
TCAATTGTACTCATTGCAAA+CGG - chr3.4:80292531-80292550 None:intergenic 30.0%
TCTTTACATAACCATTCCTA+AGG + chr3.4:80292857-80292876 MS.gene067048:CDS 30.0%
TGTGCAGTTTGAGAATATTA+AGG + chr3.4:80292815-80292834 MS.gene067048:CDS 30.0%
AAAAACTCTCTTCTTGGGTT+CGG - chr3.4:80292418-80292437 None:intergenic 35.0%
AGTTTGTCTTGATGAACTCT+TGG - chr3.4:80292307-80292326 None:intergenic 35.0%
CAATTGTACTCATTGCAAAC+GGG - chr3.4:80292530-80292549 None:intergenic 35.0%
CACTCTTTATCCTCAAATCT+TGG + chr3.4:80292261-80292280 MS.gene067048:CDS 35.0%
GAATAAGTAGTAGTAGCCTT+AGG - chr3.4:80292876-80292895 None:intergenic 35.0%
GAATATTAAGGCTGAGGTTA+AGG + chr3.4:80292827-80292846 MS.gene067048:CDS 35.0%
GAGAAAGTGAATACAAGATG+AGG + chr3.4:80292631-80292650 MS.gene067048:CDS 35.0%
GATGTTTAAATCTGGTTGTG+AGG + chr3.4:80293022-80293041 MS.gene067048:intron 35.0%
GTTTGAGAATATTAAGGCTG+AGG + chr3.4:80292821-80292840 MS.gene067048:CDS 35.0%
TGATGAACTCTTGGAAATCA+TGG - chr3.4:80292298-80292317 None:intergenic 35.0%
TTCTCAAACTGCACATTCTT+TGG - chr3.4:80292810-80292829 None:intergenic 35.0%
TTGCAATGAGTACAATTGAG+TGG + chr3.4:80292532-80292551 MS.gene067048:CDS 35.0%
AACTCTTGGAAATCATGGAC+AGG - chr3.4:80292293-80292312 None:intergenic 40.0%
AAGATGAGGTTTGATATGCC+TGG + chr3.4:80292645-80292664 MS.gene067048:CDS 40.0%
AATCGATCCCATAACCCTAT+CGG - chr3.4:80292450-80292469 None:intergenic 40.0%
AATGGTCTAAGAGCTATGGT+AGG + chr3.4:80292763-80292782 MS.gene067048:CDS 40.0%
ACATCCTCTTTGTTCATACC+AGG - chr3.4:80292666-80292685 None:intergenic 40.0%
AGAGGATGTTAAAGTTTGGG+TGG + chr3.4:80292677-80292696 MS.gene067048:CDS 40.0%
AGCAGGAAAAACTCTCTTCT+TGG - chr3.4:80292424-80292443 None:intergenic 40.0%
ATTAAGGCTGAGGTTAAGGA+TGG + chr3.4:80292831-80292850 MS.gene067048:CDS 40.0%
CAGGTTAAGACCAAGATTTG+AGG - chr3.4:80292274-80292293 None:intergenic 40.0%
GAAGAATGGTCTAAGAGCTA+TGG + chr3.4:80292759-80292778 MS.gene067048:CDS 40.0%
GCAGGAAAAACTCTCTTCTT+GGG - chr3.4:80292423-80292442 None:intergenic 40.0%
TATGCCTGGTATGAACAAAG+AGG + chr3.4:80292659-80292678 MS.gene067048:CDS 40.0%
TTAAGGCTGAGGTTAAGGAT+GGG + chr3.4:80292832-80292851 MS.gene067048:CDS 40.0%
! AGTAGTAGTAGCCTTAGGAA+TGG - chr3.4:80292871-80292890 None:intergenic 40.0%
! GGAAACAATGGAAAGCATGA+TGG + chr3.4:80292500-80292519 MS.gene067048:CDS 40.0%
!! GCAAGAGATGATGGAAACAA+TGG + chr3.4:80292488-80292507 MS.gene067048:CDS 40.0%
AAGGACAGTGCAAGAGATGA+TGG + chr3.4:80292479-80292498 MS.gene067048:CDS 45.0%
AAGGAGTAGGTGGGTATAGA+AGG + chr3.4:80292577-80292596 MS.gene067048:CDS 45.0%
ACACCATGGGAGGTTAAAGA+AGG + chr3.4:80292609-80292628 MS.gene067048:CDS 45.0%
AGTAGGTGGGTATAGAAGGA+GGG + chr3.4:80292581-80292600 MS.gene067048:CDS 45.0%
TAAGGCTGAGGTTAAGGATG+GGG + chr3.4:80292833-80292852 MS.gene067048:CDS 45.0%
TACCCACCTACTCCTTCAAT+CGG - chr3.4:80292573-80292592 None:intergenic 45.0%
TCTCCTTCTTTAACCTCCCA+TGG - chr3.4:80292615-80292634 None:intergenic 45.0%
TTCTCTTGCTTCACCTGTCA+TGG - chr3.4:80292349-80292368 None:intergenic 45.0%
TTGGGTGGAAGAGAAGATGT+TGG + chr3.4:80292692-80292711 MS.gene067048:CDS 45.0%
! GGGATCGATTTCTTACGACA+AGG + chr3.4:80292460-80292479 MS.gene067048:CDS 45.0%
! TACCGATTGAAGGAGTAGGT+GGG + chr3.4:80292568-80292587 MS.gene067048:CDS 45.0%
!! TTTTTCCTGCTGCACCGATA+GGG + chr3.4:80292433-80292452 MS.gene067048:CDS 45.0%
AAGGAGGGGAAGAACACCAT+GGG + chr3.4:80292596-80292615 MS.gene067048:CDS 50.0%
CCTACTCCTTCAATCGGTAG+CGG - chr3.4:80292567-80292586 None:intergenic 50.0%
CTTCGAGTCAAAGCCATGAC+AGG + chr3.4:80292333-80292352 MS.gene067048:CDS 50.0%
GAGTAGGTGGGTATAGAAGG+AGG + chr3.4:80292580-80292599 MS.gene067048:CDS 50.0%
GTAGGTGGGTATAGAAGGAG+GGG + chr3.4:80292582-80292601 MS.gene067048:CDS 50.0%
! CTACCGATTGAAGGAGTAGG+TGG + chr3.4:80292567-80292586 MS.gene067048:CDS 50.0%
! TGCTGCACCGATAGGGTTAT+GGG + chr3.4:80292440-80292459 MS.gene067048:CDS 50.0%
!! CTGTCATGGCTTTGACTCGA+AGG - chr3.4:80292335-80292354 None:intergenic 50.0%
!! GTTTTTCCTGCTGCACCGAT+AGG + chr3.4:80292432-80292451 MS.gene067048:CDS 50.0%
CATAACCCTATCGGTGCAGC+AGG - chr3.4:80292441-80292460 None:intergenic 55.0%
CTCTCTTCTTGGGTTCGGAC+TGG - chr3.4:80292413-80292432 None:intergenic 55.0%
GAAGGAGGGGAAGAACACCA+TGG + chr3.4:80292595-80292614 MS.gene067048:CDS 55.0%
! CCGCTACCGATTGAAGGAGT+AGG + chr3.4:80292564-80292583 MS.gene067048:CDS 55.0%
! CTGCTGCACCGATAGGGTTA+TGG + chr3.4:80292439-80292458 MS.gene067048:CDS 55.0%
! TCAATCGGTAGCGGCGATGA+AGG - chr3.4:80292558-80292577 None:intergenic 55.0%
! TCATCGCCGCTACCGATTGA+AGG + chr3.4:80292558-80292577 MS.gene067048:CDS 55.0%
! TCTTCTTGGGTTCGGACTGG+TGG - chr3.4:80292410-80292429 None:intergenic 55.0%
GAGGGGAAGAACACCATGGG+AGG + chr3.4:80292599-80292618 MS.gene067048:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 80292255 80293061 80292255 ID=MS.gene067048
chr3.4 mRNA 80292255 80293061 80292255 ID=MS.gene067048.t1;Parent=MS.gene067048
chr3.4 exon 80292255 80292914 80292255 ID=MS.gene067048.t1.exon1;Parent=MS.gene067048.t1
chr3.4 CDS 80292255 80292914 80292255 ID=cds.MS.gene067048.t1;Parent=MS.gene067048.t1
chr3.4 exon 80293023 80293061 80293023 ID=MS.gene067048.t1.exon2;Parent=MS.gene067048.t1
chr3.4 CDS 80293023 80293061 80293023 ID=cds.MS.gene067048.t1;Parent=MS.gene067048.t1
Gene Sequence

>MS.gene067048

ATGGCACACTCTTTATCCTCAAATCTTGGTCTTAACCTGTCCATGATTTCCAAGAGTTCATCAAGACAAACTCATTTCCTTCGAGTCAAAGCCATGACAGGTGAAGCAAGAGAAAAACTTGACCATGTTTCAAGATCAAACAATATTAAACACCACCAGTCCGAACCCAAGAAGAGAGTTTTTCCTGCTGCACCGATAGGGTTATGGGATCGATTTCTTACGACAAGGACAGTGCAAGAGATGATGGAAACAATGGAAAGCATGATGGAAGACCCGTTTGCAATGAGTACAATTGAGTGGCCTTCATCGCCGCTACCGATTGAAGGAGTAGGTGGGTATAGAAGGAGGGGAAGAACACCATGGGAGGTTAAAGAAGGAGAAAGTGAATACAAGATGAGGTTTGATATGCCTGGTATGAACAAAGAGGATGTTAAAGTTTGGGTGGAAGAGAAGATGTTGGTTGTGAAAGCTGAGAAAGCATCCAAAAAGAAGAATGATGAAGATGAAGAATGGTCTAAGAGCTATGGTAGGTATAGTAGCAGAATTGCTTTGCCAAAGAATGTGCAGTTTGAGAATATTAAGGCTGAGGTTAAGGATGGGGTTCTTTACATAACCATTCCTAAGGCTACTACTACTTATTCCAAAGTCCTTGATCTTAATGTTCAATGACAATCATTCATTTTTTTTCCCTGGAGTTGTTAATTAAGCTTTTGTTATTTTTGATATATATTTAGATTTTTATATTGTTTTACGAACCCTTTTTGTAAGATGTTTAAATCTGGTTGTGAGGAAGCAGCTCTATTATGA

Protein sequence

>MS.gene067048.t1

MAHSLSSNLGLNLSMISKSSSRQTHFLRVKAMTGEAREKLDHVSRSNNIKHHQSEPKKRVFPAAPIGLWDRFLTTRTVQEMMETMESMMEDPFAMSTIEWPSSPLPIEGVGGYRRRGRTPWEVKEGESEYKMRFDMPGMNKEDVKVWVEEKMLVVKAEKASKKKNDEDEEWSKSYGRYSSRIALPKNVQFENIKAEVKDGVLYITIPKATTTYSKVLDLNMFKSGCEEAALL