AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0331740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331740 MtrunA17_Chr2g0333051 81.481 135 7 1 1 117 1 135 6.30e-66 195
Msa0331740 MtrunA17_Chr4g0003391 45.038 131 49 4 1 116 1 123 1.60e-22 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331740 AT1G52565.1 41.538 130 56 5 1 117 4 126 6.71e-14 63.5
Msa0331740 AT3G15760.1 38.686 137 59 6 1 117 1 132 2.31e-13 62.4

Find 27 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCATCATTTCAAATTCT+TGG 0.191389 2_4:+47714885 Msa0331740:CDS
TACAAAGCCATTCTATTTGC+AGG 0.298881 2_4:-47715506 None:intergenic
CATCACTAGTTATTTGAGAT+TGG 0.337453 2_4:+47715782 Msa0331740:three_prime_UTR
CTTCCAAGAATTTGAAATGA+TGG 0.348228 2_4:-47714888 None:intergenic
GATAAAATACACTGCTTCAT+AGG 0.352001 2_4:-47715602 None:intergenic
TGATGGGTTAATCTTGTGTT+TGG 0.357672 2_4:-47714871 None:intergenic
ATAGAATGGCTTTGTATAAC+GGG 0.410953 2_4:+47715513 Msa0331740:CDS
TTGTGATAAACTAAATTAAG+TGG 0.420140 2_4:+47715857 Msa0331740:three_prime_UTR
GATTCTCGTTGAATGAAACT+TGG 0.436395 2_4:+47715567 Msa0331740:exon
TGGTCAATGTAATTTAGTTA+AGG 0.438504 2_4:+47715877 Msa0331740:three_prime_UTR
TCAAATAACTAGTGATGAGT+TGG 0.448071 2_4:-47715776 None:intergenic
AATAGAATGGCTTTGTATAA+CGG 0.457196 2_4:+47715512 Msa0331740:CDS
TTCCAAGAATTTGAAATGAT+GGG 0.463098 2_4:-47714887 None:intergenic
TTGAATGAAACTTGGGAAAG+AGG 0.491240 2_4:+47715575 Msa0331740:three_prime_UTR
ACAAATGACTTCTCTCTCAC+GGG 0.491614 2_4:+47714791 None:intergenic
CTGTTGTGTGGTTGTAGTGT+GGG 0.501190 2_4:+47715718 Msa0331740:three_prime_UTR
TAGATTTGAAGAAAGTGTGA+AGG 0.513088 2_4:+47715382 Msa0331740:CDS
TGGAATTCCTGCAAATAGAA+TGG 0.533064 2_4:+47715499 Msa0331740:CDS
AACAAATGACTTCTCTCTCA+CGG 0.539460 2_4:+47714790 None:intergenic
TTTCTCAGAGGAAAAGAAGA+GGG 0.546313 2_4:+47715322 Msa0331740:intron
ATTCTCGTTGAATGAAACTT+GGG 0.558587 2_4:+47715568 Msa0331740:exon
ACTGTTGTGTGGTTGTAGTG+TGG 0.565398 2_4:+47715717 Msa0331740:three_prime_UTR
CATAATGTCAAATCTAGTTG+TGG 0.615424 2_4:-47715751 None:intergenic
AGTGAAACAACGAAAACTCG+TGG 0.634965 2_4:+47715479 Msa0331740:CDS
TGTTGTGTGGTTGTAGTGTG+GGG 0.661609 2_4:+47715719 Msa0331740:three_prime_UTR
ACACATACACAACTGTTGTG+TGG 0.669472 2_4:+47715706 Msa0331740:three_prime_UTR
CAAATGACTTCTCTCTCACG+GGG 0.697931 2_4:+47714792 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCGATATTGATATTAATTA+AGG - chr2_4:47715206-47715225 None:intergenic 15.0%
!! ATCGATATTGATATTAATTA+AGG - chr2_4:47715206-47715225 None:intergenic 15.0%
!! TCTACATACAAAATAAAGTA+AGG - chr2_4:47714995-47715014 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + chr2_4:47714994-47715013 Msa0331740:intron 20.0%
!! ATATCGATATTGATATTGTT+AGG - chr2_4:47715111-47715130 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + chr2_4:47715211-47715230 Msa0331740:intron 20.0%
!! AAAATACACACAATATAGTA+AGG - chr2_4:47715302-47715321 None:intergenic 20.0%
!! TTGTGATAAACTAAATTAAG+TGG + chr2_4:47715857-47715876 Msa0331740:three_prime_UTR 20.0%
!! TCTACATACAAAATAAAGTA+AGG - chr2_4:47714995-47715014 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + chr2_4:47714994-47715013 Msa0331740:intron 20.0%
!! ATATCGATATTGATATTGTT+AGG - chr2_4:47715111-47715130 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + chr2_4:47715211-47715230 Msa0331740:intron 20.0%
!! AAAATACACACAATATAGTA+AGG - chr2_4:47715302-47715321 None:intergenic 20.0%
!! TTGTGATAAACTAAATTAAG+TGG + chr2_4:47715857-47715876 Msa0331740:three_prime_UTR 20.0%
!! TTCCAAGAATTTGAAATGAT+GGG - chr2_4:47714890-47714909 None:intergenic 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - chr2_4:47715152-47715171 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + chr2_4:47715512-47715531 Msa0331740:CDS 25.0%
!!! TGAATTCTAGTTTAGTTTTG+TGG + chr2_4:47715672-47715691 Msa0331740:three_prime_UTR 25.0%
! TGGTCAATGTAATTTAGTTA+AGG + chr2_4:47715877-47715896 Msa0331740:three_prime_UTR 25.0%
!! TTCCAAGAATTTGAAATGAT+GGG - chr2_4:47714890-47714909 None:intergenic 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - chr2_4:47715152-47715171 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + chr2_4:47715512-47715531 Msa0331740:CDS 25.0%
!!! TGAATTCTAGTTTAGTTTTG+TGG + chr2_4:47715672-47715691 Msa0331740:three_prime_UTR 25.0%
! TGGTCAATGTAATTTAGTTA+AGG + chr2_4:47715877-47715896 Msa0331740:three_prime_UTR 25.0%
AACCCATCATTTCAAATTCT+TGG + chr2_4:47714885-47714904 Msa0331740:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - chr2_4:47714891-47714910 None:intergenic 30.0%
ATAGTAAGGACTAAGAACTA+AGG - chr2_4:47715288-47715307 None:intergenic 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + chr2_4:47715310-47715329 Msa0331740:intron 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + chr2_4:47715382-47715401 Msa0331740:CDS 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + chr2_4:47715426-47715445 Msa0331740:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + chr2_4:47715513-47715532 Msa0331740:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + chr2_4:47715523-47715542 Msa0331740:CDS 30.0%
ATTCTCGTTGAATGAAACTT+GGG + chr2_4:47715568-47715587 Msa0331740:exon 30.0%
GATAAAATACACTGCTTCAT+AGG - chr2_4:47715605-47715624 None:intergenic 30.0%
TCAGAGAAAAACAAAAACAG+AGG - chr2_4:47715645-47715664 None:intergenic 30.0%
!!! TGTTTTTGTTTTTCTCTGAG+TGG + chr2_4:47715646-47715665 Msa0331740:three_prime_UTR 30.0%
CATAATGTCAAATCTAGTTG+TGG - chr2_4:47715754-47715773 None:intergenic 30.0%
TCAAATAACTAGTGATGAGT+TGG - chr2_4:47715779-47715798 None:intergenic 30.0%
! CATCACTAGTTATTTGAGAT+TGG + chr2_4:47715782-47715801 Msa0331740:three_prime_UTR 30.0%
AACCCATCATTTCAAATTCT+TGG + chr2_4:47714885-47714904 Msa0331740:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - chr2_4:47714891-47714910 None:intergenic 30.0%
ATAGTAAGGACTAAGAACTA+AGG - chr2_4:47715288-47715307 None:intergenic 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + chr2_4:47715310-47715329 Msa0331740:intron 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + chr2_4:47715382-47715401 Msa0331740:CDS 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + chr2_4:47715426-47715445 Msa0331740:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + chr2_4:47715513-47715532 Msa0331740:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + chr2_4:47715523-47715542 Msa0331740:CDS 30.0%
ATTCTCGTTGAATGAAACTT+GGG + chr2_4:47715568-47715587 Msa0331740:exon 30.0%
GATAAAATACACTGCTTCAT+AGG - chr2_4:47715605-47715624 None:intergenic 30.0%
TCAGAGAAAAACAAAAACAG+AGG - chr2_4:47715645-47715664 None:intergenic 30.0%
!!! TGTTTTTGTTTTTCTCTGAG+TGG + chr2_4:47715646-47715665 Msa0331740:three_prime_UTR 30.0%
CATAATGTCAAATCTAGTTG+TGG - chr2_4:47715754-47715773 None:intergenic 30.0%
TCAAATAACTAGTGATGAGT+TGG - chr2_4:47715779-47715798 None:intergenic 30.0%
! CATCACTAGTTATTTGAGAT+TGG + chr2_4:47715782-47715801 Msa0331740:three_prime_UTR 30.0%
GGAAAAAGCTAGATAAAGCA+TGG - chr2_4:47714853-47714872 None:intergenic 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - chr2_4:47714874-47714893 None:intergenic 35.0%
AGCGCAAAATTTGTGTGTTA+GGG - chr2_4:47714929-47714948 None:intergenic 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + chr2_4:47714942-47714961 Msa0331740:intron 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - chr2_4:47714975-47714994 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_4:47715182-47715201 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_4:47715182-47715201 None:intergenic 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + chr2_4:47715321-47715340 Msa0331740:intron 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + chr2_4:47715322-47715341 Msa0331740:intron 35.0%
TGGAATTCCTGCAAATAGAA+TGG + chr2_4:47715499-47715518 Msa0331740:CDS 35.0%
TACAAAGCCATTCTATTTGC+AGG - chr2_4:47715509-47715528 None:intergenic 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + chr2_4:47715522-47715541 Msa0331740:CDS 35.0%
GATTCTCGTTGAATGAAACT+TGG + chr2_4:47715567-47715586 Msa0331740:exon 35.0%
TTGAATGAAACTTGGGAAAG+AGG + chr2_4:47715575-47715594 Msa0331740:three_prime_UTR 35.0%
!!! TCTAGTTTAGTTTTGTGGCA+AGG + chr2_4:47715677-47715696 Msa0331740:three_prime_UTR 35.0%
!!! CTAGTTTAGTTTTGTGGCAA+GGG + chr2_4:47715678-47715697 Msa0331740:three_prime_UTR 35.0%
GGAAAAAGCTAGATAAAGCA+TGG - chr2_4:47714853-47714872 None:intergenic 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - chr2_4:47714874-47714893 None:intergenic 35.0%
AGCGCAAAATTTGTGTGTTA+GGG - chr2_4:47714929-47714948 None:intergenic 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + chr2_4:47714942-47714961 Msa0331740:intron 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - chr2_4:47714975-47714994 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_4:47715182-47715201 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - chr2_4:47715182-47715201 None:intergenic 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + chr2_4:47715321-47715340 Msa0331740:intron 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + chr2_4:47715322-47715341 Msa0331740:intron 35.0%
TGGAATTCCTGCAAATAGAA+TGG + chr2_4:47715499-47715518 Msa0331740:CDS 35.0%
TACAAAGCCATTCTATTTGC+AGG - chr2_4:47715509-47715528 None:intergenic 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + chr2_4:47715522-47715541 Msa0331740:CDS 35.0%
GATTCTCGTTGAATGAAACT+TGG + chr2_4:47715567-47715586 Msa0331740:exon 35.0%
TTGAATGAAACTTGGGAAAG+AGG + chr2_4:47715575-47715594 Msa0331740:three_prime_UTR 35.0%
!!! TCTAGTTTAGTTTTGTGGCA+AGG + chr2_4:47715677-47715696 Msa0331740:three_prime_UTR 35.0%
!!! CTAGTTTAGTTTTGTGGCAA+GGG + chr2_4:47715678-47715697 Msa0331740:three_prime_UTR 35.0%
GAGCGCAAAATTTGTGTGTT+AGG - chr2_4:47714930-47714949 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + chr2_4:47715028-47715047 Msa0331740:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + chr2_4:47715139-47715158 Msa0331740:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + chr2_4:47715221-47715240 Msa0331740:intron 40.0%
AGTGAAACAACGAAAACTCG+TGG + chr2_4:47715479-47715498 Msa0331740:CDS 40.0%
ACACATACACAACTGTTGTG+TGG + chr2_4:47715706-47715725 Msa0331740:three_prime_UTR 40.0%
GAGCGCAAAATTTGTGTGTT+AGG - chr2_4:47714930-47714949 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + chr2_4:47715028-47715047 Msa0331740:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + chr2_4:47715139-47715158 Msa0331740:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + chr2_4:47715221-47715240 Msa0331740:intron 40.0%
AGTGAAACAACGAAAACTCG+TGG + chr2_4:47715479-47715498 Msa0331740:CDS 40.0%
ACACATACACAACTGTTGTG+TGG + chr2_4:47715706-47715725 Msa0331740:three_prime_UTR 40.0%
ACACACAACTGTGTGGTACA+GGG + chr2_4:47715060-47715079 Msa0331740:intron 45.0%
!! TTTTGAGGGATCGTTTGCAC+AGG + chr2_4:47715537-47715556 Msa0331740:CDS 45.0%
ACTGTTGTGTGGTTGTAGTG+TGG + chr2_4:47715717-47715736 Msa0331740:three_prime_UTR 45.0%
CTGTTGTGTGGTTGTAGTGT+GGG + chr2_4:47715718-47715737 Msa0331740:three_prime_UTR 45.0%
TGTTGTGTGGTTGTAGTGTG+GGG + chr2_4:47715719-47715738 Msa0331740:three_prime_UTR 45.0%
ACACACAACTGTGTGGTACA+GGG + chr2_4:47715060-47715079 Msa0331740:intron 45.0%
!! TTTTGAGGGATCGTTTGCAC+AGG + chr2_4:47715537-47715556 Msa0331740:CDS 45.0%
ACTGTTGTGTGGTTGTAGTG+TGG + chr2_4:47715717-47715736 Msa0331740:three_prime_UTR 45.0%
CTGTTGTGTGGTTGTAGTGT+GGG + chr2_4:47715718-47715737 Msa0331740:three_prime_UTR 45.0%
TGTTGTGTGGTTGTAGTGTG+GGG + chr2_4:47715719-47715738 Msa0331740:three_prime_UTR 45.0%
CGAACTCACACACAACTGTG+TGG + chr2_4:47715053-47715072 Msa0331740:intron 50.0%
CACACACAACTGTGTGGTAC+AGG + chr2_4:47715059-47715078 Msa0331740:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + chr2_4:47715071-47715090 Msa0331740:intron 50.0%
CGAACTCACACACAACTGTG+TGG + chr2_4:47715053-47715072 Msa0331740:intron 50.0%
CACACACAACTGTGTGGTAC+AGG + chr2_4:47715059-47715078 Msa0331740:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + chr2_4:47715071-47715090 Msa0331740:intron 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 47714795 47715916 47714795 ID=Msa0331740;Name=Msa0331740
chr2_4 mRNA 47714795 47715916 47714795 ID=Msa0331740-mRNA-1;Parent=Msa0331740;Name=Msa0331740-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0.5|1|0|0.5|2|348|117
chr2_4 exon 47714795 47714909 47714795 ID=Msa0331740-mRNA-1:exon:14363;Parent=Msa0331740-mRNA-1
chr2_4 exon 47715330 47715916 47715330 ID=Msa0331740-mRNA-1:exon:14364;Parent=Msa0331740-mRNA-1
chr2_4 CDS 47714795 47714909 47714795 ID=Msa0331740-mRNA-1:cds;Parent=Msa0331740-mRNA-1
chr2_4 CDS 47715330 47715568 47715330 ID=Msa0331740-mRNA-1:cds;Parent=Msa0331740-mRNA-1
chr2_4 three_prime_UTR 47715569 47715916 47715569 ID=Msa0331740-mRNA-1:three_prime_utr;Parent=Msa0331740-mRNA-1
Gene Sequence

>Msa0331740

ATGACTTCTCTCTCACGGGGTCTTGTTTTCACTACAGCTATGCTTGTATCAAGCACCATGCTTTATCTAGCTTTTTCCAAACACAAGATTAACCCATCATTTCAAATTCTTGGAAAGGAAAAGAAGAGGGAGAGAAAGATGAATACAAAGAAAAATAAAAAGAAAGTTAGATTTGAAGAAAGTGTGAAGGAAGTAAGAGAAAAGAGTGAGATAGCAAAAAAAGAGAAACAGAGGAAACGAAACAGAGTACATAGCAACTGCAGAAGTGAAACAACGAAAACTCGTGGAATTCCTGCAAATAGAATGGCTTTGTATAACGGGATTTTGAGGGATCGTTTGCACAGGATTGAGTGA

Protein sequence

>Msa0331740

MTSLSRGLVFTTAMLVSSTMLYLAFSKHKINPSFQILGKEKKRERKMNTKKNKKKVRFEESVKEVREKSEIAKKEKQRKRNRVHSNCRSETTKTRGIPANRMALYNGILRDRLHRIE*