AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06288


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06288.t1 MTR_3g098520 95.597 159 6 1 1 158 1 159 9.07e-111 311
MS.gene06288.t1 MTR_3g098520 94.681 94 4 1 1 93 1 94 2.61e-60 182
MS.gene06288.t1 MTR_5g008050 50.000 60 24 2 55 110 181 238 2.76e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06288.t1 AT5G51010 60.377 159 55 4 1 157 1 153 9.33e-61 185
MS.gene06288.t1 AT5G17170 41.584 101 33 5 55 150 180 259 6.78e-13 64.7

Find 48 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCAATTGCTTCATCTCTT+TGG 0.237136 3.1:+78080438 None:intergenic
GTTGATTAGGATGAAGTAAA+AGG 0.290725 3.1:+78081322 None:intergenic
AATCAACTACAAGTTTCTTA+TGG 0.292009 3.1:-78081306 MS.gene06288:CDS
TAAGAAACTTGTAGTTGATT+AGG 0.306861 3.1:+78081309 None:intergenic
CCAGGGCAGAAGTATTTATC+AGG 0.317364 3.1:+78081095 None:intergenic
AAAGATGTCTTGAGAGTAGA+TGG 0.349045 3.1:+78081363 None:intergenic
TAGGATGAAGTAAAAGGTTA+AGG 0.356489 3.1:+78081328 None:intergenic
ACGACAGATATAAGCTTGTT+TGG 0.361476 3.1:+78081254 None:intergenic
GTGGGAGTTGTAGTTCTTGC+AGG 0.375454 3.1:-78080292 MS.gene06288:CDS
CTCCAAAGAGATGAAGCAAT+TGG 0.389726 3.1:-78080440 MS.gene06288:CDS
ACTCCCACGGCAACTGCAAT+AGG 0.395621 3.1:+78080307 None:intergenic
ATAGGGCTTAAACCTTCGTT+TGG 0.408207 3.1:+78080523 None:intergenic
AGCTACACGCATGGTAAACC+TGG 0.428236 3.1:+78081278 None:intergenic
TTTGTTGACATCTGTTGCAT+AGG 0.431914 3.1:+78080505 None:intergenic
TTGTTGACATCTGTTGCATA+GGG 0.437509 3.1:+78080506 None:intergenic
TGTCTTGAGAGTAGATGGAT+TGG 0.449903 3.1:+78081368 None:intergenic
TTGGAGAGTGGTCGGAGGGT+AGG 0.455499 3.1:+78081387 None:intergenic
AGGATGAAGTAAAAGGTTAA+GGG 0.468286 3.1:+78081329 None:intergenic
TAGGGCTTAAACCTTCGTTT+GGG 0.473259 3.1:+78080524 None:intergenic
TTATCAGGCAATTTATCAAA+GGG 0.481492 3.1:+78081110 None:intergenic
GTGATAATCTTGTAGTGTGA+AGG 0.484953 3.1:+78081421 None:intergenic
TCCAAAGAGATGAAGCAATT+GGG 0.489224 3.1:-78080439 MS.gene06288:intron
TAGATGGATTGGAGAGTGGT+CGG 0.491971 3.1:+78081379 None:intergenic
TGATAATCTTGTAGTGTGAA+GGG 0.498122 3.1:+78081422 None:intergenic
CTTATATCTGTCGTGACTGC+GGG 0.498593 3.1:-78081245 MS.gene06288:intron
TTTATCAGGCAATTTATCAA+AGG 0.502560 3.1:+78081109 None:intergenic
CAAAATGGCCTTGCAAGTGC+AGG 0.518248 3.1:-78081448 None:intergenic
AAATGAAACAGATGTTAGAA+AGG 0.526185 3.1:-78080477 MS.gene06288:CDS
TGGATTGGAGAGTGGTCGGA+GGG 0.526512 3.1:+78081383 None:intergenic
ACTTCCTATTGCAGTTGCCG+TGG 0.541016 3.1:-78080311 MS.gene06288:CDS
CCTGATAAATACTTCTGCCC+TGG 0.541318 3.1:-78081095 MS.gene06288:intron
AAGTTTCTTATGGACCTCCC+AGG 0.547146 3.1:-78081296 MS.gene06288:CDS
CTTCCTATTGCAGTTGCCGT+GGG 0.547663 3.1:-78080310 MS.gene06288:CDS
GAGTGGTCGGAGGGTAGGAG+TGG 0.553144 3.1:+78081392 None:intergenic
ATCAGGCAATTTATCAAAGG+GGG 0.560795 3.1:+78081112 None:intergenic
AAGGGAGGCCTGCACTTGCA+AGG 0.564120 3.1:+78081440 None:intergenic
GCAGGTATATTTACAATGAG+AGG 0.564968 3.1:-78081136 MS.gene06288:intron
GCTTATATCTGTCGTGACTG+CGG 0.577229 3.1:-78081246 MS.gene06288:CDS
CGGAGGGTAGGAGTGGCAGT+AGG 0.598226 3.1:+78081399 None:intergenic
ATGGATTGGAGAGTGGTCGG+AGG 0.602826 3.1:+78081382 None:intergenic
TTGTTTGGAAGCTACACGCA+TGG 0.631363 3.1:+78081269 None:intergenic
TTTGTGGTGCTCCCAAACGA+AGG 0.633347 3.1:-78080535 MS.gene06288:CDS
TGCAAGAACTACAACTCCCA+CGG 0.635141 3.1:+78080294 None:intergenic
AGAGTAGATGGATTGGAGAG+TGG 0.641832 3.1:+78081375 None:intergenic
TATCAGGCAATTTATCAAAG+GGG 0.660862 3.1:+78081111 None:intergenic
GCTACACGCATGGTAAACCT+GGG 0.677062 3.1:+78081279 None:intergenic
TAATCTTGTAGTGTGAAGGG+AGG 0.698956 3.1:+78081425 None:intergenic
ACACGCATGGTAAACCTGGG+AGG 0.732150 3.1:+78081282 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTATCAAATTCTTGT+TGG - chr3.1:78080737-78080756 MS.gene06288:intron 15.0%
!! ATGTCAAAAACAAAAATAAT+GGG + chr3.1:78081344-78081363 None:intergenic 15.0%
!! CATATCTATTATCTATATAT+AGG - chr3.1:78080832-78080851 MS.gene06288:intron 15.0%
!! GAAATATATATCTCATTATT+TGG - chr3.1:78080527-78080546 MS.gene06288:CDS 15.0%
!! GAATTTGATAAATATTCATA+TGG + chr3.1:78080733-78080752 None:intergenic 15.0%
!! TAATATTATACTGATAAGAA+AGG - chr3.1:78081065-78081084 MS.gene06288:intron 15.0%
!! TATGTCAAAAACAAAAATAA+TGG + chr3.1:78081345-78081364 None:intergenic 15.0%
!! AAAAAGTAATGAAATTACCA+GGG + chr3.1:78080630-78080649 None:intergenic 20.0%
!! TAAAAAGTAATGAAATTACC+AGG + chr3.1:78080631-78080650 None:intergenic 20.0%
!! TTATCTATATATAGGATGTA+TGG - chr3.1:78080840-78080859 MS.gene06288:intron 20.0%
!! TTTAAGAAGCAAAAGAAATA+CGG - chr3.1:78081102-78081121 MS.gene06288:CDS 20.0%
!!! TTTTTCAACCAAGTTTATTA+AGG - chr3.1:78081033-78081052 MS.gene06288:intron 20.0%
! AAAAGAGAATGATCAAAGAA+AGG + chr3.1:78080493-78080512 None:intergenic 25.0%
! AAATGAAACAGATGTTAGAA+AGG - chr3.1:78081228-78081247 MS.gene06288:intron 25.0%
! AATCAACTACAAGTTTCTTA+TGG - chr3.1:78080399-78080418 MS.gene06288:intron 25.0%
! ACTGATAAGAAAGGAAAAAT+AGG - chr3.1:78081074-78081093 MS.gene06288:intron 25.0%
! ACTTTAGACCTTAATAAACT+TGG + chr3.1:78081044-78081063 None:intergenic 25.0%
! TAAGAAACTTGTAGTTGATT+AGG + chr3.1:78080399-78080418 None:intergenic 25.0%
! TGATAAATATTCATATGGCA+TGG + chr3.1:78080728-78080747 None:intergenic 25.0%
! TTAAGAAGCAAAAGAAATAC+GGG - chr3.1:78081103-78081122 MS.gene06288:CDS 25.0%
! TTATCAGGCAATTTATCAAA+GGG + chr3.1:78080598-78080617 None:intergenic 25.0%
! TTTATCAGGCAATTTATCAA+AGG + chr3.1:78080599-78080618 None:intergenic 25.0%
! TTTATTATGCTAATGTCAAC+AGG - chr3.1:78081366-78081385 MS.gene06288:CDS 25.0%
!!! CAAAAACAAAAATAATGGGT+AGG + chr3.1:78081340-78081359 None:intergenic 25.0%
!!! TCTGCTTTAAGTTCTAAATT+TGG + chr3.1:78080978-78080997 None:intergenic 25.0%
!!! TTTGTTGATAATAATGTTGC+AGG - chr3.1:78080551-78080570 MS.gene06288:intron 25.0%
AAGTACAGAGAAAGAAAATC+AGG - chr3.1:78080809-78080828 MS.gene06288:intron 30.0%
AGGATGAAGTAAAAGGTTAA+GGG + chr3.1:78080379-78080398 None:intergenic 30.0%
AGGCAAAAAGAAAATGACTA+TGG + chr3.1:78081300-78081319 None:intergenic 30.0%
GCATTATGATTGTCTCAAAA+TGG - chr3.1:78081137-78081156 MS.gene06288:intron 30.0%
GTTAGGTTTAGTGAATTTGA+CGG - chr3.1:78080876-78080895 MS.gene06288:intron 30.0%
GTTGATTAGGATGAAGTAAA+AGG + chr3.1:78080386-78080405 None:intergenic 30.0%
TAGGATGAAGTAAAAGGTTA+AGG + chr3.1:78080380-78080399 None:intergenic 30.0%
TATCAGGCAATTTATCAAAG+GGG + chr3.1:78080597-78080616 None:intergenic 30.0%
TGATAATCTTGTAGTGTGAA+GGG + chr3.1:78080286-78080305 None:intergenic 30.0%
! TCTAAAATGTGTTGCCTATA+TGG + chr3.1:78080932-78080951 None:intergenic 30.0%
!! GCTTTAAGTTCTAAATTTGG+TGG + chr3.1:78080975-78080994 None:intergenic 30.0%
!! GTTGTCTTTCTATTGACTTA+AGG - chr3.1:78080764-78080783 MS.gene06288:intron 30.0%
AAAATGTGTTGCCTATATGG+CGG + chr3.1:78080929-78080948 None:intergenic 35.0%
AAAGATGTCTTGAGAGTAGA+TGG + chr3.1:78080345-78080364 None:intergenic 35.0%
AGGAGTGAACATATTAAGCA+AGG + chr3.1:78081320-78081339 None:intergenic 35.0%
ATCAGGCAATTTATCAAAGG+GGG + chr3.1:78080596-78080615 None:intergenic 35.0%
GCAGGTATATTTACAATGAG+AGG - chr3.1:78080569-78080588 MS.gene06288:intron 35.0%
GTGATAATCTTGTAGTGTGA+AGG + chr3.1:78080287-78080306 None:intergenic 35.0%
TCCAAAGAGATGAAGCAATT+GGG - chr3.1:78081266-78081285 MS.gene06288:CDS 35.0%
TGTCCACTATTACTCATAGA+TGG - chr3.1:78080660-78080679 MS.gene06288:intron 35.0%
TTGTTGACATCTGTTGCATA+GGG + chr3.1:78081202-78081221 None:intergenic 35.0%
TTTGTTGACATCTGTTGCAT+AGG + chr3.1:78081203-78081222 None:intergenic 35.0%
! ACGACAGATATAAGCTTGTT+TGG + chr3.1:78080454-78080473 None:intergenic 35.0%
ACCCAATTGCTTCATCTCTT+TGG + chr3.1:78081270-78081289 None:intergenic 40.0%
ATAGGGCTTAAACCTTCGTT+TGG + chr3.1:78081185-78081204 None:intergenic 40.0%
ATGGCTTCTAAGTGCATGTT+AGG - chr3.1:78080859-78080878 MS.gene06288:intron 40.0%
CCACTATTACTCATAGATGG+AGG - chr3.1:78080663-78080682 MS.gene06288:intron 40.0%
CCTCCATCTATGAGTAATAG+TGG + chr3.1:78080666-78080685 None:intergenic 40.0%
CGGTGAATTTGACAGAATCA+CGG - chr3.1:78080896-78080915 MS.gene06288:intron 40.0%
CTCCAAAGAGATGAAGCAAT+TGG - chr3.1:78081265-78081284 MS.gene06288:CDS 40.0%
TAATCTTGTAGTGTGAAGGG+AGG + chr3.1:78080283-78080302 None:intergenic 40.0%
TAGGGCTTAAACCTTCGTTT+GGG + chr3.1:78081184-78081203 None:intergenic 40.0%
TGAATTTGACAGAATCACGG+TGG - chr3.1:78080899-78080918 MS.gene06288:intron 40.0%
TGTCTTGAGAGTAGATGGAT+TGG + chr3.1:78080340-78080359 None:intergenic 40.0%
! AAATGGCGTTTTGCAGTTTG+TGG - chr3.1:78081154-78081173 MS.gene06288:intron 40.0%
AAGTTTCTTATGGACCTCCC+AGG - chr3.1:78080409-78080428 MS.gene06288:intron 45.0%
ATGAGTCCAGTCTTTGTGAG+TGG + chr3.1:78081010-78081029 None:intergenic 45.0%
CCTGATAAATACTTCTGCCC+TGG - chr3.1:78080610-78080629 MS.gene06288:intron 45.0%
CTTATATCTGTCGTGACTGC+GGG - chr3.1:78080460-78080479 MS.gene06288:CDS 45.0%
GCTTATATCTGTCGTGACTG+CGG - chr3.1:78080459-78080478 MS.gene06288:CDS 45.0%
TGCAAGAACTACAACTCCCA+CGG + chr3.1:78081414-78081433 None:intergenic 45.0%
TTGTTTGGAAGCTACACGCA+TGG + chr3.1:78080439-78080458 None:intergenic 45.0%
!! AGAGTAGATGGATTGGAGAG+TGG + chr3.1:78080333-78080352 None:intergenic 45.0%
!! CCAGGGCAGAAGTATTTATC+AGG + chr3.1:78080613-78080632 None:intergenic 45.0%
!! TAGATGGATTGGAGAGTGGT+CGG + chr3.1:78080329-78080348 None:intergenic 45.0%
ACTTCCTATTGCAGTTGCCG+TGG - chr3.1:78081394-78081413 MS.gene06288:CDS 50.0%
AGCTACACGCATGGTAAACC+TGG + chr3.1:78080430-78080449 None:intergenic 50.0%
CTTCCTATTGCAGTTGCCGT+GGG - chr3.1:78081395-78081414 MS.gene06288:CDS 50.0%
GCTACACGCATGGTAAACCT+GGG + chr3.1:78080429-78080448 None:intergenic 50.0%
GTGGGAGTTGTAGTTCTTGC+AGG - chr3.1:78081413-78081432 MS.gene06288:CDS 50.0%
TCACTGCCACTCACAAAGAC+TGG - chr3.1:78081001-78081020 MS.gene06288:intron 50.0%
TTTGTGGTGCTCCCAAACGA+AGG - chr3.1:78081170-78081189 MS.gene06288:intron 50.0%
!! ACGGTGGATTACCGCCATAT+AGG - chr3.1:78080915-78080934 MS.gene06288:intron 50.0%
ACACGCATGGTAAACCTGGG+AGG + chr3.1:78080426-78080445 None:intergenic 55.0%
ACTCCCACGGCAACTGCAAT+AGG + chr3.1:78081401-78081420 None:intergenic 55.0%
! TGGATTGGAGAGTGGTCGGA+GGG + chr3.1:78080325-78080344 None:intergenic 55.0%
!! ATGGATTGGAGAGTGGTCGG+AGG + chr3.1:78080326-78080345 None:intergenic 55.0%
AAGGGAGGCCTGCACTTGCA+AGG + chr3.1:78080268-78080287 None:intergenic 60.0%
TTGGAGAGTGGTCGGAGGGT+AGG + chr3.1:78080321-78080340 None:intergenic 60.0%
CGGAGGGTAGGAGTGGCAGT+AGG + chr3.1:78080309-78080328 None:intergenic 65.0%
GAGTGGTCGGAGGGTAGGAG+TGG + chr3.1:78080316-78080335 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 78080261 78081466 78080261 ID=MS.gene06288
chr3.1 mRNA 78080261 78081466 78080261 ID=MS.gene06288.t1;Parent=MS.gene06288
chr3.1 exon 78081246 78081466 78081246 ID=MS.gene06288.t1.exon1;Parent=MS.gene06288.t1
chr3.1 CDS 78081246 78081466 78081246 ID=cds.MS.gene06288.t1;Parent=MS.gene06288.t1
chr3.1 exon 78081096 78081154 78081096 ID=MS.gene06288.t1.exon2;Parent=MS.gene06288.t1
chr3.1 CDS 78081096 78081154 78081096 ID=cds.MS.gene06288.t1;Parent=MS.gene06288.t1
chr3.1 exon 78080440 78080557 78080440 ID=MS.gene06288.t1.exon3;Parent=MS.gene06288.t1
chr3.1 CDS 78080440 78080557 78080440 ID=cds.MS.gene06288.t1;Parent=MS.gene06288.t1
chr3.1 exon 78080261 78080339 78080261 ID=MS.gene06288.t1.exon4;Parent=MS.gene06288.t1
chr3.1 CDS 78080261 78080339 78080261 ID=cds.MS.gene06288.t1;Parent=MS.gene06288.t1
Gene Sequence

>MS.gene06288

ATGGCCTTGCAAGTGCAGGCCTCCCTTCACACTACAAGATTATCACCTACTGCCACTCCTACCCTCCGACCACTCTCCAATCCATCTACTCTCAAGACATCTTTCTTCTCTCGTTCCCTTAACCTTTTACTTCATCCTAATCAACTACAAGTTTCTTATGGACCTCCCAGGTTTACCATGCGTGTAGCTTCCAAACAAGCTTATATCTGTCGTGACTGCGGGTTATTTTCCTTTCTTTGATCATTCTCTTTTTAGTATATCTGAATGAAATATATATCTCATTATTTGGTTTTGTTGATAATAATGTTGCAGGTATATTTACAATGAGAGGACCCCCTTTGATAAATTGCCTGATAAATACTTCTGCCCTGGTAATTTCATTACTTTTTACTCATATAGTGTCCACTATTACTCATAGATGGAGGAACTTCATTTCAAAACTATAATATTTGCTTTTACGCTTTCCATGCCATATGAATATTTATCAAATTCTTGTTGGTTTAGTTGTCTTTCTATTGACTTAAGGTATACTATTAAATTTCTCTTATAAGTACAGAGAAAGAAAATCAGGCATATCTATTATCTATATATAGGATGTATGGCTTCTAAGTGCATGTTAGGTTTAGTGAATTTGACGGTGAATTTGACAGAATCACGGTGGATTACCGCCATATAGGCAACACATTTTAGAGTTTCAAAAAAATCATAGTGCCACCAAATTTAGAACTTAAAGCAGAGCTTCACTGCCACTCACAAAGACTGGACTCATTTGTTTTTCAACCAAGTTTATTAAGGTCTAAAGTCTAATATTATACTGATAAGAAAGGAAAAATAGGACTTTTTTAAGAAGCAAAAGAAATACGGGTCAATGAGACTGCATTATGATTGTCTCAAAATGGCGTTTTGCAGTTTGTGGTGCTCCCAAACGAAGGTTTAAGCCCTATGCAACAGATGTCAACAAAAAAGCAAATGAAACAGATGTTAGAAAGGCAAGAAAAGCTGAACTCCAAAGAGATGAAGCAATTGGGTGAGTGAGCCATAGTCATTTTCTTTTTGCCTTGCTTAATATGTTCACTCCTACCCATTATTTTTGTTTTTGACATAATTTATTATGCTAATGTCAACAGGAAAGCACTTCCTATTGCAGTTGCCGTGGGAGTTGTAGTTCTTGCAGGATTATACTTCTATCTCAACAACACATTTTAG

Protein sequence

>MS.gene06288.t1

MALQVQASLHTTRLSPTATPTLRPLSNPSTLKTSFFSRSLNLLLHPNQLQVSYGPPRFTMRVASKQAYICRDCGYIYNERTPFDKLPDKYFCPVCGAPKRRFKPYATDVNKKANETDVRKARKAELQRDEAIGKALPIAVAVGVVVLAGLYFYLNNTF