AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52273


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52273.t1 MTR_7g080793 92.661 109 5 2 1 108 1 107 7.80e-71 206
MS.gene52273.t1 MTR_7g080803 69.091 110 30 3 1 108 1 108 1.33e-46 145
MS.gene52273.t1 MTR_5g008460 68.182 110 31 3 1 108 1 108 2.66e-46 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52273.t1 AT4G10040 68.182 110 31 3 1 108 1 108 5.45e-46 144
MS.gene52273.t1 AT1G22840 66.364 110 33 3 1 108 1 108 3.81e-44 139
MS.gene52273.t1 AT1G22840 63.636 88 29 3 1 86 1 87 2.17e-31 107

Find 39 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCACCGGCAGCACGGTTTC+TGG 0.212914 7.4:+25689407 None:intergenic
ATCAATGTGTTGTACCTTCT+TGG 0.250097 7.4:+25689039 None:intergenic
TCAATGTGTTGTACCTTCTT+GGG 0.277885 7.4:+25689040 None:intergenic
TCTTATTGCATATCTGAAAT+AGG 0.298515 7.4:-25688714 None:intergenic
CCCAATCTGAATGGTTTGTT+TGG 0.305862 7.4:-25689163 MS.gene52273:CDS
ACAAGAACATGTCTATGAAT+TGG 0.322531 7.4:-25689096 MS.gene52273:CDS
GGTTTGTTTGGAAGTCAATC+TGG 0.344506 7.4:-25689151 MS.gene52273:CDS
GTGCTGCCGGTGAGAAGTTC+AGG 0.352217 7.4:-25689398 MS.gene52273:CDS
GCAACACAATATGAATATTC+AGG 0.355918 7.4:+25689121 None:intergenic
GATTATCACGTTCCTGGCCC+TGG 0.377704 7.4:+25688735 None:intergenic
TCACCGGCAGCACGGTTTCT+GGG 0.386514 7.4:+25689408 None:intergenic
CAAGAACATGTCTATGAATT+GGG 0.403729 7.4:-25689095 MS.gene52273:CDS
CAATAAGATTATCACGTTCC+TGG 0.419432 7.4:+25688729 None:intergenic
TCCAAACAAACCATTCAGAT+TGG 0.443064 7.4:+25689162 None:intergenic
ATTATCACGTTCCTGGCCCT+GGG 0.444706 7.4:+25688736 None:intergenic
GTACCTTCTTGGGGTTAAGC+AGG 0.466044 7.4:+25689050 None:intergenic
TCCTGCTCTTAAGAAGCCCC+AGG 0.485526 7.4:-25688753 MS.gene52273:CDS
TCTTAAGAAGCCCCAGGGCC+AGG 0.486959 7.4:-25688747 MS.gene52273:CDS
CCAAACAAACCATTCAGATT+GGG 0.496498 7.4:+25689163 None:intergenic
CCCTGGGGCTTCTTAAGAGC+AGG 0.499405 7.4:+25688752 None:intergenic
TATTAAACCTTGTTTGTGAA+CGG 0.502480 7.4:+25689341 None:intergenic
TTTGTGAACGGCTCCTATGT+TGG 0.508283 7.4:+25689353 None:intergenic
CTTCCCAGAAACCGTGCTGC+CGG 0.509379 7.4:-25689411 MS.gene52273:CDS
GTTTGTTTGGAAGTCAATCT+GGG 0.526603 7.4:-25689150 MS.gene52273:CDS
CAATGTGTTGTACCTTCTTG+GGG 0.536623 7.4:+25689041 None:intergenic
ACCTCAGGACCCAATCTGAA+TGG 0.540730 7.4:-25689172 MS.gene52273:intron
GCTCAATGCCACACCAACAT+AGG 0.557024 7.4:-25689366 MS.gene52273:CDS
GAATTGGGAGGAAAAGATCA+TGG 0.562314 7.4:-25689080 MS.gene52273:CDS
GCACGGTTTCTGGGAAGTGT+CGG 0.562820 7.4:+25689417 None:intergenic
GCACATCCTCGATACGAAGA+TGG 0.574573 7.4:-25688780 MS.gene52273:CDS
ATAGGAGCCGTTCACAAACA+AGG 0.604312 7.4:-25689348 MS.gene52273:intron
TGAACTTCTCACCGGCAGCA+CGG 0.620657 7.4:+25689400 None:intergenic
ATCACTCCTGAACTTCTCAC+CGG 0.651892 7.4:+25689392 None:intergenic
GAACATGTCTATGAATTGGG+AGG 0.658810 7.4:-25689092 MS.gene52273:CDS
GAAATACCATCTTCGTATCG+AGG 0.660566 7.4:+25688774 None:intergenic
TTACCTGCTTAACCCCAAGA+AGG 0.666741 7.4:-25689053 MS.gene52273:intron
CCTGCTCTTAAGAAGCCCCA+GGG 0.667349 7.4:-25688752 MS.gene52273:CDS
TTATCACGTTCCTGGCCCTG+GGG 0.669948 7.4:+25688737 None:intergenic
GAACGGCTCCTATGTTGGTG+TGG 0.672356 7.4:+25689358 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAATCATAATAAATTAA+TGG + chr7.4:25689273-25689292 None:intergenic 10.0%
!! TAAAGAATATTTATATCAAA+TGG - chr7.4:25689160-25689179 MS.gene52273:CDS 10.0%
!! CATCATATCATAATATTCTA+AGG - chr7.4:25688924-25688943 MS.gene52273:intron 20.0%
!! GTAAATATGAGAGTTTATTT+AGG + chr7.4:25688848-25688867 None:intergenic 20.0%
!! TTTATTATGATTATACTGAG+AGG - chr7.4:25689277-25689296 MS.gene52273:intron 20.0%
! TTCATAGTAGAAAACAACAA+AGG + chr7.4:25689238-25689257 None:intergenic 25.0%
!! TATTAAACCTTGTTTGTGAA+CGG + chr7.4:25688809-25688828 None:intergenic 25.0%
ACAAGAACATGTCTATGAAT+TGG - chr7.4:25689051-25689070 MS.gene52273:intron 30.0%
ATGATGACATATATTCAAGC+AGG + chr7.4:25688910-25688929 None:intergenic 30.0%
CAAGAACATGTCTATGAATT+GGG - chr7.4:25689052-25689071 MS.gene52273:intron 30.0%
GCAACACAATATGAATATTC+AGG + chr7.4:25689029-25689048 None:intergenic 30.0%
TAATCGACATAGACTTTCAA+GGG + chr7.4:25689306-25689325 None:intergenic 30.0%
TTAATCGACATAGACTTTCA+AGG + chr7.4:25689307-25689326 None:intergenic 30.0%
!!! TTTTATTTGTTGTCAACCTC+AGG - chr7.4:25688960-25688979 MS.gene52273:intron 30.0%
ATCAATGTGTTGTACCTTCT+TGG + chr7.4:25689111-25689130 None:intergenic 35.0%
CAATAAGATTATCACGTTCC+TGG + chr7.4:25689421-25689440 None:intergenic 35.0%
CCAAACAAACCATTCAGATT+GGG + chr7.4:25688987-25689006 None:intergenic 35.0%
GTTTGTTTGGAAGTCAATCT+GGG - chr7.4:25688997-25689016 MS.gene52273:intron 35.0%
TCAATGTGTTGTACCTTCTT+GGG + chr7.4:25689110-25689129 None:intergenic 35.0%
TCCAAACAAACCATTCAGAT+TGG + chr7.4:25688988-25689007 None:intergenic 35.0%
! AGAAGGTACAACACATTGAT+TGG - chr7.4:25689111-25689130 MS.gene52273:CDS 35.0%
CAATGTGTTGTACCTTCTTG+GGG + chr7.4:25689109-25689128 None:intergenic 40.0%
GAAATACCATCTTCGTATCG+AGG + chr7.4:25689376-25689395 None:intergenic 40.0%
GAACATGTCTATGAATTGGG+AGG - chr7.4:25689055-25689074 MS.gene52273:CDS 40.0%
GAATTGGGAGGAAAAGATCA+TGG - chr7.4:25689067-25689086 MS.gene52273:CDS 40.0%
! ATATATTCAAGCAGGAGCAC+AGG + chr7.4:25688902-25688921 None:intergenic 40.0%
! GGTTTGTTTGGAAGTCAATC+TGG - chr7.4:25688996-25689015 MS.gene52273:intron 40.0%
!! CCCAATCTGAATGGTTTGTT+TGG - chr7.4:25688984-25689003 MS.gene52273:intron 40.0%
ATAGGAGCCGTTCACAAACA+AGG - chr7.4:25688799-25688818 MS.gene52273:intron 45.0%
ATCACTCCTGAACTTCTCAC+CGG + chr7.4:25688758-25688777 None:intergenic 45.0%
TTACCTGCTTAACCCCAAGA+AGG - chr7.4:25689094-25689113 MS.gene52273:CDS 45.0%
TTTGTGAACGGCTCCTATGT+TGG + chr7.4:25688797-25688816 None:intergenic 45.0%
ACCATTCAGATTGGGTCCTG+AGG + chr7.4:25688979-25688998 None:intergenic 50.0%
ACCTCAGGACCCAATCTGAA+TGG - chr7.4:25688975-25688994 MS.gene52273:intron 50.0%
ATTATCACGTTCCTGGCCCT+GGG + chr7.4:25689414-25689433 None:intergenic 50.0%
GCACATCCTCGATACGAAGA+TGG - chr7.4:25689367-25689386 MS.gene52273:CDS 50.0%
GCTCAATGCCACACCAACAT+AGG - chr7.4:25688781-25688800 MS.gene52273:CDS 50.0%
GTACCTTCTTGGGGTTAAGC+AGG + chr7.4:25689100-25689119 None:intergenic 50.0%
CCTGCTCTTAAGAAGCCCCA+GGG - chr7.4:25689395-25689414 MS.gene52273:CDS 55.0%
GAACGGCTCCTATGTTGGTG+TGG + chr7.4:25688792-25688811 None:intergenic 55.0%
GATTATCACGTTCCTGGCCC+TGG + chr7.4:25689415-25689434 None:intergenic 55.0%
TCCTGCTCTTAAGAAGCCCC+AGG - chr7.4:25689394-25689413 MS.gene52273:CDS 55.0%
TGAACTTCTCACCGGCAGCA+CGG + chr7.4:25688750-25688769 None:intergenic 55.0%
TTATCACGTTCCTGGCCCTG+GGG + chr7.4:25689413-25689432 None:intergenic 55.0%
! GCACGGTTTCTGGGAAGTGT+CGG + chr7.4:25688733-25688752 None:intergenic 55.0%
CCCTGGGGCTTCTTAAGAGC+AGG + chr7.4:25689398-25689417 None:intergenic 60.0%
CTTCCCAGAAACCGTGCTGC+CGG - chr7.4:25688736-25688755 MS.gene52273:CDS 60.0%
GTGCTGCCGGTGAGAAGTTC+AGG - chr7.4:25688749-25688768 MS.gene52273:CDS 60.0%
TCACCGGCAGCACGGTTTCT+GGG + chr7.4:25688742-25688761 None:intergenic 60.0%
TCTTAAGAAGCCCCAGGGCC+AGG - chr7.4:25689400-25689419 MS.gene52273:CDS 60.0%
CTCACCGGCAGCACGGTTTC+TGG + chr7.4:25688743-25688762 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 25688715 25689454 25688715 ID=MS.gene52273
chr7.4 mRNA 25688715 25689454 25688715 ID=MS.gene52273.t1;Parent=MS.gene52273
chr7.4 exon 25689349 25689454 25689349 ID=MS.gene52273.t1.exon1;Parent=MS.gene52273.t1
chr7.4 CDS 25689349 25689454 25689349 ID=cds.MS.gene52273.t1;Parent=MS.gene52273.t1
chr7.4 exon 25689054 25689187 25689054 ID=MS.gene52273.t1.exon2;Parent=MS.gene52273.t1
chr7.4 CDS 25689054 25689187 25689054 ID=cds.MS.gene52273.t1;Parent=MS.gene52273.t1
chr7.4 exon 25688715 25688801 25688715 ID=MS.gene52273.t1.exon3;Parent=MS.gene52273.t1
chr7.4 CDS 25688715 25688801 25688715 ID=cds.MS.gene52273.t1;Parent=MS.gene52273.t1
Gene Sequence

>MS.gene52273

ATGGAGTTGTTCGAACCGACACTTCCCAGAAACCGTGCTGCCGGTGAGAAGTTCAGGAGTGATTGCGCTCAATGCCACACCAACATAGGAGCCGTTCACAAACAAGGTTTAATATTAGTATTCTTTTCTGCCTAAATAAACTCTCATATTTACGTATCATGTGCTACTTAATTAGTTGCAGTTTCCTGTGCTCCTGCTTGAATATATGTCATCATATCATAATATTCTAAGGATAAAAATGTTTGTTTTATTTGTTGTCAACCTCAGGACCCAATCTGAATGGTTTGTTTGGAAGTCAATCTGGGACAACTCCTGAATATTCATATTGTGTTGCTAACAAGAACATGTCTATGAATTGGGAGGAAAAGATCATGGATGATTACCTGCTTAACCCCAAGAAGGTACAACACATTGATTGGCATTTCAATGTTTCTTTTTAGATCAATAAAGAATATTTATATCAAATGGTTATTAATAATCGCTTGATGCTTCTTTGTATATAATAATATTATCATTCTAACCTTTGTTGTTTTCTACTATGAATAAGCTCTTGTTCCATTAATTTATTATGATTATACTGAGAGGTAACCCTTGAAAGTCTATGTCGATTAAATGATCACTGATTTGTGTATTATTTTTTCATTCTTATGTAGCACATCCTCGATACGAAGATGGTATTTCCTGCTCTTAAGAAGCCCCAGGGCCAGGAACGTGATAATCTTATTGCATATCTGAAATAG

Protein sequence

>MS.gene52273.t1

MELFEPTLPRNRAAGEKFRSDCAQCHTNIGAVHKQGPNLNGLFGSQSGTTPEYSYCVANKNMSMNWEEKIMDDYLLNPKKHILDTKMVFPALKKPQGQERDNLIAYLK