AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58779


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58779.t1 MTR_4g116350 95.152 165 8 0 2 166 4 168 7.81e-104 298
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58779.t1 AT2G47910 59.880 167 57 3 10 166 7 173 6.48e-64 196
MS.gene58779.t1 AT2G47910 72.222 108 27 1 62 166 18 125 1.25e-52 166

Find 41 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACGTTGATGACGAGAAAT+TGG 0.265812 8.3:+9586142 None:intergenic
GAAGCAATGGTCTGAATCTA+AGG 0.301805 8.3:+9586224 None:intergenic
AGAGGGGAAGTGGTTTGTTT+TGG 0.313531 8.3:+9586184 None:intergenic
GAATTTCTTGAGCGCTCAAT+TGG 0.362235 8.3:-9585137 MS.gene58779:CDS
AGTATCCTGATATTAGACTA+TGG 0.405584 8.3:-9585061 MS.gene58779:CDS
ACAAACCATAGTCTAATATC+AGG 0.408448 8.3:+9585056 None:intergenic
AGGGAAAAGCATGAAGTTAG+AGG 0.409056 8.3:+9586166 None:intergenic
ATGCCAGTTGCAGCTTGAAA+AGG 0.412295 8.3:+9585295 None:intergenic
GCTCTCCAATCCAATAAAAC+TGG 0.419972 8.3:+9585005 None:intergenic
TGCCAGTTGCAGCTTGAAAA+GGG 0.422401 8.3:+9585296 None:intergenic
ATGATTTGCGTACCAGATAA+GGG 0.429757 8.3:+9585265 None:intergenic
AATGATTTGCGTACCAGATA+AGG 0.441596 8.3:+9585264 None:intergenic
GAAGTTTCCTTGTGGGTTGA+AGG 0.453295 8.3:+9586099 None:intergenic
GTCTCTTTATTTGATGATGA+TGG 0.456703 8.3:-9586073 MS.gene58779:intron
GCATGAAGTTAGAGGGGAAG+TGG 0.457683 8.3:+9586174 None:intergenic
TCGAATCGGCCTTCAGTTGG+AGG 0.473327 8.3:+9585361 None:intergenic
ATCTTGTCGTGTATAGTTGA+TGG 0.474003 8.3:+9585109 None:intergenic
TCCAATAAAACTGGCAACCA+TGG 0.487310 8.3:+9585014 None:intergenic
GACTTGATGCTGCTTATCCA+TGG 0.492069 8.3:-9585031 MS.gene58779:CDS
CGGCCTTCAGTTGGAGGCAT+AGG 0.503641 8.3:+9585367 None:intergenic
TATCAATTACAACTTCGAAT+CGG 0.519861 8.3:+9585347 None:intergenic
AGACATCGAAGTTTCCTTGT+GGG 0.528434 8.3:+9586092 None:intergenic
ACTTCGAATCGGCCTTCAGT+TGG 0.531614 8.3:+9585358 None:intergenic
TTTGGCAAAGGAGATGAAAG+TGG 0.539719 8.3:+9586202 None:intergenic
GGGAAAAGCATGAAGTTAGA+GGG 0.542061 8.3:+9586167 None:intergenic
CCTTCAGTTGGAGGCATAGG+AGG 0.545720 8.3:+9585370 None:intergenic
GGAGAGCTGGAGAGCTAGCT+CGG 0.572069 8.3:-9584989 MS.gene58779:intron
GGAAAAGCATGAAGTTAGAG+GGG 0.574365 8.3:+9586168 None:intergenic
GGAGATGAAAGTGGAAGCAA+TGG 0.582269 8.3:+9586211 None:intergenic
CCTCCTATGCCTCCAACTGA+AGG 0.594495 8.3:-9585370 MS.gene58779:CDS
GTTGATGACGAGAAATTGGA+AGG 0.599353 8.3:+9586146 None:intergenic
GCCAGTTGCAGCTTGAAAAG+GGG 0.608468 8.3:+9585297 None:intergenic
GAGACATCGAAGTTTCCTTG+TGG 0.622426 8.3:+9586091 None:intergenic
GATAATTCTCGAGGATCGCG+AGG 0.628859 8.3:+9585086 None:intergenic
CTTGAAAAGGGGTTAAGTCA+AGG 0.630683 8.3:+9585308 None:intergenic
GAGCGCTCAAGAAATTCTTG+TGG 0.636416 8.3:+9585143 None:intergenic
TTGATGACGAGAAATTGGAA+GGG 0.641000 8.3:+9586147 None:intergenic
AAGCAATGGTCTGAATCTAA+GGG 0.645376 8.3:+9586225 None:intergenic
GGATACTCTGATAATTCTCG+AGG 0.647549 8.3:+9585077 None:intergenic
TCTGTTGCCTTCAACCCACA+AGG 0.654997 8.3:-9586106 MS.gene58779:CDS
CCAATTTCTCGTCATCAACG+TGG 0.655284 8.3:-9586142 MS.gene58779:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GACAAATGATATAAAAAAAA+AGG + chr8.3:9584892-9584911 None:intergenic 15.0%
!! GAACTTATTATACTAAACAA+TGG - chr8.3:9585499-9585518 MS.gene58779:intron 20.0%
!! TTAGATAAAAGTCTTCAAAT+TGG + chr8.3:9585076-9585095 None:intergenic 20.0%
!!! AAAAGATTAGTTCATTATGT+GGG - chr8.3:9585238-9585257 MS.gene58779:intron 20.0%
!!! ACTTTTATATAGGAAATAAG+AGG - chr8.3:9586185-9586204 MS.gene58779:CDS 20.0%
!!! AGTACTATGGTTTTAAATTA+CGG - chr8.3:9586010-9586029 MS.gene58779:intron 20.0%
!!! CTTTTATATAGGAAATAAGA+GGG - chr8.3:9586186-9586205 MS.gene58779:CDS 20.0%
!!! TGTACTAACTTTTACAATAA+AGG + chr8.3:9585330-9585349 None:intergenic 20.0%
!!! TGTCTTAATTGCTTTAATTT+TGG - chr8.3:9585689-9585708 MS.gene58779:intron 20.0%
!!! TTGAATTTTATTCCTGTATT+TGG - chr8.3:9585013-9585032 MS.gene58779:CDS 20.0%
! ATTTATTGTATTACCTGATG+CGG + chr8.3:9585972-9585991 None:intergenic 25.0%
! CAAATTGGTATTAAGTCATA+AGG + chr8.3:9585061-9585080 None:intergenic 25.0%
! TAACAATTACGGATTTCATT+GGG + chr8.3:9585188-9585207 None:intergenic 25.0%
! TAAGTCATAAGGTATATGTT+GGG + chr8.3:9585050-9585069 None:intergenic 25.0%
! TAATTCTCTCTGCATTTAAA+TGG - chr8.3:9585477-9585496 MS.gene58779:intron 25.0%
! TACTATAGTGTTAGATATGT+TGG - chr8.3:9584978-9584997 MS.gene58779:intron 25.0%
! TATCAATTACAACTTCGAAT+CGG + chr8.3:9585573-9585592 None:intergenic 25.0%
! TTAAGTCATAAGGTATATGT+TGG + chr8.3:9585051-9585070 None:intergenic 25.0%
!! ACTCAGTAGTACTTTTATAT+AGG - chr8.3:9586175-9586194 MS.gene58779:CDS 25.0%
!! CAAAAGATTAGTTCATTATG+TGG - chr8.3:9585237-9585256 MS.gene58779:intron 25.0%
!! GAAGACTTTTATCTAAATGT+GGG - chr8.3:9585080-9585099 MS.gene58779:CDS 25.0%
!! TGAAGACTTTTATCTAAATG+TGG - chr8.3:9585079-9585098 MS.gene58779:CDS 25.0%
!! TTTTACTACTGAGATAACTT+AGG + chr8.3:9585401-9585420 None:intergenic 25.0%
!!! ACCTCTAAAATCTTTGTATT+AGG - chr8.3:9586120-9586139 MS.gene58779:CDS 25.0%
!!! CCTCTAAAATCTTTGTATTA+GGG - chr8.3:9586121-9586140 MS.gene58779:CDS 25.0%
ACAAACCATAGTCTAATATC+AGG + chr8.3:9585864-9585883 None:intergenic 30.0%
AGTACTTTAATTCAGCCTAT+AGG - chr8.3:9585140-9585159 MS.gene58779:CDS 30.0%
AGTATCCTGATATTAGACTA+TGG - chr8.3:9585856-9585875 MS.gene58779:intron 30.0%
CAATGAAATCCGTAATTGTT+AGG - chr8.3:9585187-9585206 MS.gene58779:intron 30.0%
CTAACAATTACGGATTTCAT+TGG + chr8.3:9585189-9585208 None:intergenic 30.0%
TTAAATTACGGTTGTGGTTA+TGG - chr8.3:9586022-9586041 MS.gene58779:intron 30.0%
TTGACTCAAAGTGAAAAAAG+TGG + chr8.3:9585377-9585396 None:intergenic 30.0%
TTTGTGTATGAGTAGTACTA+TGG - chr8.3:9585997-9586016 MS.gene58779:intron 30.0%
! CCCTAATACAAAGATTTTAG+AGG + chr8.3:9586124-9586143 None:intergenic 30.0%
!! GTCTCTTTATTTGATGATGA+TGG - chr8.3:9584844-9584863 MS.gene58779:intron 30.0%
!!! ATGGTTTTAAATTACGGTTG+TGG - chr8.3:9586016-9586035 MS.gene58779:intron 30.0%
AAATGAACTTAGATGCTTCC+TGG - chr8.3:9585729-9585748 MS.gene58779:intron 35.0%
AAGCAATGGTCTGAATCTAA+GGG + chr8.3:9584695-9584714 None:intergenic 35.0%
ACTGTAGTAATGATAACACC+AGG + chr8.3:9585750-9585769 None:intergenic 35.0%
AGCTTCAGTCCTAACAATTA+CGG + chr8.3:9585199-9585218 None:intergenic 35.0%
ATCTTGTCGTGTATAGTTGA+TGG + chr8.3:9585811-9585830 None:intergenic 35.0%
GCAAACATTCATCCAAATAC+AGG + chr8.3:9585028-9585047 None:intergenic 35.0%
! AATGATTTGCGTACCAGATA+AGG + chr8.3:9585656-9585675 None:intergenic 35.0%
! AGGTTAGAATTAGTCTCACA+AGG + chr8.3:9585310-9585329 None:intergenic 35.0%
! AGTGTTAGATATGTTGGTCT+TGG - chr8.3:9584984-9585003 MS.gene58779:intron 35.0%
! ATGATTTGCGTACCAGATAA+GGG + chr8.3:9585655-9585674 None:intergenic 35.0%
!! TACTACTGAGTTCTGAATTG+TGG + chr8.3:9586166-9586185 None:intergenic 35.0%
!! TTGATGACGAGAAATTGGAA+GGG + chr8.3:9584773-9584792 None:intergenic 35.0%
!!! TAAATGTGGGTTTTTTAGCC+TGG - chr8.3:9585093-9585112 MS.gene58779:CDS 35.0%
AGACATCGAAGTTTCCTTGT+GGG + chr8.3:9584828-9584847 None:intergenic 40.0%
CCACAAAAATGGCTACCTAT+AGG + chr8.3:9585158-9585177 None:intergenic 40.0%
CGTTATCACAATCGCAATTG+TGG + chr8.3:9586092-9586111 None:intergenic 40.0%
CTTGAAAAGGGGTTAAGTCA+AGG + chr8.3:9585612-9585631 None:intergenic 40.0%
GAAGCAATGGTCTGAATCTA+AGG + chr8.3:9584696-9584715 None:intergenic 40.0%
GAATTTCTTGAGCGCTCAAT+TGG - chr8.3:9585780-9585799 MS.gene58779:intron 40.0%
GCTCTCCAATCCAATAAAAC+TGG + chr8.3:9585915-9585934 None:intergenic 40.0%
GGATACTCTGATAATTCTCG+AGG + chr8.3:9585843-9585862 None:intergenic 40.0%
GGCATGAAAAATCCCTTATC+TGG - chr8.3:9585640-9585659 MS.gene58779:intron 40.0%
TCCAATAAAACTGGCAACCA+TGG + chr8.3:9585906-9585925 None:intergenic 40.0%
TTTGGCAAAGGAGATGAAAG+TGG + chr8.3:9584718-9584737 None:intergenic 40.0%
! AGGGAAAAGCATGAAGTTAG+AGG + chr8.3:9584754-9584773 None:intergenic 40.0%
! ATTAAAGTACTCCACTCCAC+TGG + chr8.3:9585131-9585150 None:intergenic 40.0%
! GGAAAAGCATGAAGTTAGAG+GGG + chr8.3:9584752-9584771 None:intergenic 40.0%
! GGGAAAAGCATGAAGTTAGA+GGG + chr8.3:9584753-9584772 None:intergenic 40.0%
!! GTTGATGACGAGAAATTGGA+AGG + chr8.3:9584774-9584793 None:intergenic 40.0%
!! GTTTTATTGGATTGGAGAGC+TGG - chr8.3:9585915-9585934 MS.gene58779:intron 40.0%
!! TCCATGGTTGCCAGTTTTAT+TGG - chr8.3:9585902-9585921 MS.gene58779:intron 40.0%
!!! CCTATAGGTAGCCATTTTTG+TGG - chr8.3:9585155-9585174 MS.gene58779:intron 40.0%
!!! GAAGTGGTTTGTTTTGGCAA+AGG + chr8.3:9584730-9584749 None:intergenic 40.0%
!!! GGTTGCCAGTTTTATTGGAT+TGG - chr8.3:9585907-9585926 MS.gene58779:intron 40.0%
ATGCCAGTTGCAGCTTGAAA+AGG + chr8.3:9585625-9585644 None:intergenic 45.0%
CCAATTTCTCGTCATCAACG+TGG - chr8.3:9584775-9584794 MS.gene58779:intron 45.0%
GAGACATCGAAGTTTCCTTG+TGG + chr8.3:9584829-9584848 None:intergenic 45.0%
GAGCGCTCAAGAAATTCTTG+TGG + chr8.3:9585777-9585796 None:intergenic 45.0%
GGAGATGAAAGTGGAAGCAA+TGG + chr8.3:9584709-9584728 None:intergenic 45.0%
TGCCAGTTGCAGCTTGAAAA+GGG + chr8.3:9585624-9585643 None:intergenic 45.0%
TGCTATGTTAGTTCCGCATC+AGG - chr8.3:9585956-9585975 MS.gene58779:intron 45.0%
TGGGAATCGTGCCACAAAAA+TGG + chr8.3:9585169-9585188 None:intergenic 45.0%
! GAAGTTTCCTTGTGGGTTGA+AGG + chr8.3:9584821-9584840 None:intergenic 45.0%
! GACTTGATGCTGCTTATCCA+TGG - chr8.3:9585886-9585905 MS.gene58779:intron 45.0%
!! CCACGTTGATGACGAGAAAT+TGG + chr8.3:9584778-9584797 None:intergenic 45.0%
!!! AGAGGGGAAGTGGTTTGTTT+TGG + chr8.3:9584736-9584755 None:intergenic 45.0%
!!! GTTTTTTAGCCTGGACTTGG+TGG - chr8.3:9585102-9585121 MS.gene58779:CDS 45.0%
!!! TGGGTTTTTTAGCCTGGACT+TGG - chr8.3:9585099-9585118 MS.gene58779:CDS 45.0%
ACTTCGAATCGGCCTTCAGT+TGG + chr8.3:9585562-9585581 None:intergenic 50.0%
GATAATTCTCGAGGATCGCG+AGG + chr8.3:9585834-9585853 None:intergenic 50.0%
GCATGAAGTTAGAGGGGAAG+TGG + chr8.3:9584746-9584765 None:intergenic 50.0%
GCCAGTTGCAGCTTGAAAAG+GGG + chr8.3:9585623-9585642 None:intergenic 50.0%
TCTGTTGCCTTCAACCCACA+AGG - chr8.3:9584811-9584830 MS.gene58779:intron 50.0%
!! ACCCCTTTTCAAGCTGCAAC+TGG - chr8.3:9585619-9585638 MS.gene58779:intron 50.0%
CACTGGATACCACCAAGTCC+AGG + chr8.3:9585114-9585133 None:intergenic 55.0%
CCTCCTATGCCTCCAACTGA+AGG - chr8.3:9585547-9585566 MS.gene58779:intron 55.0%
GTGGTCCGCAATCGCAATTG+CGG + chr8.3:9586147-9586166 None:intergenic 55.0%
TAGGGCCGCAATTGCGATTG+CGG - chr8.3:9586139-9586158 MS.gene58779:CDS 55.0%
TCGAATCGGCCTTCAGTTGG+AGG + chr8.3:9585559-9585578 None:intergenic 55.0%
! CCTTCAGTTGGAGGCATAGG+AGG + chr8.3:9585550-9585569 None:intergenic 55.0%
! CTTGGTGGTATCCAGTGGAG+TGG - chr8.3:9585117-9585136 MS.gene58779:CDS 55.0%
!! CTGGACTTGGTGGTATCCAG+TGG - chr8.3:9585112-9585131 MS.gene58779:CDS 55.0%
GGAGAGCTGGAGAGCTAGCT+CGG - chr8.3:9585928-9585947 MS.gene58779:intron 60.0%
! CGGCCTTCAGTTGGAGGCAT+AGG + chr8.3:9585553-9585572 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 9584682 9586257 9584682 ID=MS.gene58779
chr8.3 mRNA 9584682 9586257 9584682 ID=MS.gene58779.t1;Parent=MS.gene58779
chr8.3 exon 9586074 9586257 9586074 ID=MS.gene58779.t1.exon1;Parent=MS.gene58779.t1
chr8.3 CDS 9586074 9586257 9586074 ID=cds.MS.gene58779.t1;Parent=MS.gene58779.t1
chr8.3 exon 9585278 9585409 9585278 ID=MS.gene58779.t1.exon2;Parent=MS.gene58779.t1
chr8.3 CDS 9585278 9585409 9585278 ID=cds.MS.gene58779.t1;Parent=MS.gene58779.t1
chr8.3 exon 9584990 9585170 9584990 ID=MS.gene58779.t1.exon3;Parent=MS.gene58779.t1
chr8.3 CDS 9584990 9585170 9584990 ID=cds.MS.gene58779.t1;Parent=MS.gene58779.t1
chr8.3 exon 9584682 9584685 9584682 ID=MS.gene58779.t1.exon4;Parent=MS.gene58779.t1
chr8.3 CDS 9584682 9584685 9584682 ID=cds.MS.gene58779.t1;Parent=MS.gene58779.t1
Gene Sequence

>MS.gene58779

ATGGCTACTACCCTTAGATTCAGACCATTGCTTCCACTTTCATCTCCTTTGCCAAAACAAACCACTTCCCCTCTAACTTCATGCTTTTCCCTTCCAATTTCTCGTCATCAACGTGGAAATGTTGCAGTCTCTGTTGCCTTCAACCCACAAGGAAACTTCGATGTCTCTTTATTTGATGATGATGGTAATATCACTTATGTTAGTACACCTTTTTTTTTATATCATTTGTCATCATATACATATGTTACTATATTAGACATTGTCATGCTATATAATTATAACGAATTGATGAGTAATACTATAGTGTTAGATATGTTGGTCTTGGTGTATTTTGAATTTTATTCCTGTATTTGGATGAATGTTTGCCCAACATATACCTTATGACTTAATACCAATTTGAAGACTTTTATCTAAATGTGGGTTTTTTAGCCTGGACTTGGTGGTATCCAGTGGAGTGGAGTACTTTAATTCAGCCTATAGGTAGCCATTTTTGTGGCACGATTCCCAATGAAATCCGTAATTGTTAGGACTGAAGCTCGTGAAGAGAGTCTAACTCAAAAGATTAGTTCATTATGTGGGAGAGAACATCATAACTTTAGTACTACATTGATCACTTTTATCTTCTCCTTGTGAGACTAATTCTAACCTTTATTGTAAAAGTTAGTACATGTTTTTCAACTTTTAATCAAAATCCACTTTTTTCACTTTGAGTCAAACCTAAGTTATCTCAGTAGTAAAACATGTGTAGTTATTAAAATCACATTCGATAGAGAGTCACAATCAATTTGACAATGATAATTCTCTCTGCATTTAAATGGAACTTATTATACTAAACAATGGTTTTGCAGATGAGTCTAAAGTTTCACCTCCTATGCCTCCAACTGAAGGCCGATTCGAAGTTGTAATTGATAATGATGCTATTAGTCGCCTTGACTTAACCCCTTTTCAAGCTGCAACTGGCATGAAAAATCCCTTATCTGGTACGCAAATCATTGCAACTTCAAAATTGTCTTAATTGCTTTAATTTTGGATATTAACTTTCACAATAAATGAACTTAGATGCTTCCTGGTGTTATCATTACTACAGTTGAACCACAAGAATTTCTTGAGCGCTCAATTGGATTTACCATCAACTATACACGACAAGATCCTCGCGATCCTCGAGAATTATCAGAGTATCCTGATATTAGACTATGGTTTGTTAGACTTGATGCTGCTTATCCATGGTTGCCAGTTTTATTGGATTGGAGAGCTGGAGAGCTAGCTCGGTATGCTGCTATGTTAGTTCCGCATCAGGTAATACAATAAATGCATGTTTGTGTATGAGTAGTACTATGGTTTTAAATTACGGTTGTGGTTATGGTGCGAACTTTGATATTGCAGCAAAATGAGCTTGATACAATACAACCACAATTGCGATTGTGATAACGTTGCTAAGACCTCTAAAATCTTTGTATTAGGGCCGCAATTGCGATTGCGGACCACAATTCAGAACTCAGTAGTACTTTTATATAGGAAATAAGAGGGCGCATCTGATACTAACTAACTTGATACTTGTATGTTCTGTTGCAGATGA

Protein sequence

>MS.gene58779.t1

MATTLRFRPLLPLSSPLPKQTTSPLTSCFSLPISRHQRGNVAVSVAFNPQGNFDVSLFDDDDESKVSPPMPPTEGRFEVVIDNDAISRLDLTPFQAATGMKNPLSVEPQEFLERSIGFTINYTRQDPRDPRELSEYPDIRLWFVRLDAAYPWLPVLLDWRAGELAR