AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065561


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065561.t1 MTR_2g064650 97.561 82 2 0 1 82 55 136 6.94e-53 161
MS.gene065561.t1 MTR_2g064650 98.077 52 1 0 1 52 55 106 2.69e-32 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065561.t1 AT1G79040 79.268 82 17 0 1 82 59 140 1.69e-40 130

Find 29 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCGACTGGCTTGGCTATC+TGG 0.093434 2.2:+22268237 MS.gene065561:CDS
CAGTCCTTTGGGTCGTAGAT+AGG 0.323072 2.2:-22267627 None:intergenic
CCGGTTGGAGACCAGTCCTT+TGG 0.338674 2.2:-22267639 None:intergenic
CCAGATGGAAAAGATGCTTC+TGG 0.381325 2.2:+22266286 MS.gene065561:CDS
CGGTTGGAGACCAGTCCTTT+GGG 0.408090 2.2:-22267638 None:intergenic
CACCGTATTTGTCCACAAAC+TGG 0.410816 2.2:-22267187 None:intergenic
TCCGACTGGCTTGGCTATCT+GGG 0.439109 2.2:+22268238 MS.gene065561:CDS
AAAGGGTGTTTACCAGTTTG+TGG 0.454085 2.2:+22267175 MS.gene065561:CDS
GTGCTAATGTTGATGGATAC+AGG 0.463270 2.2:+22267207 MS.gene065561:CDS
AAATACGGTGCTAATGTTGA+TGG 0.464376 2.2:+22267200 MS.gene065561:CDS
TACCAGTTTGTGGACAAATA+CGG 0.473942 2.2:+22267185 MS.gene065561:CDS
TTATTAATTGCAGGTCCGAC+TGG 0.479017 2.2:+22268224 MS.gene065561:intron
ATCTGGGCAGTAACTCTAGC+TGG 0.496777 2.2:+22268254 MS.gene065561:CDS
CCAAAGGACTGGTCTCCAAC+CGG 0.502102 2.2:+22267639 MS.gene065561:CDS
ACTCTAGCTGGTCTTCTAGC+TGG 0.504630 2.2:+22268266 MS.gene065561:CDS
AGTCCTTTGGGTCGTAGATA+GGG 0.509259 2.2:-22267626 None:intergenic
AATTGCAGGTCCGACTGGCT+TGG 0.514526 2.2:+22268229 MS.gene065561:intron
TGCTTCTGGCAGGAAACAAA+AGG 0.517286 2.2:+22266300 MS.gene065561:CDS
ATGGAAAAGATGCTTCTGGC+AGG 0.542571 2.2:+22266290 MS.gene065561:CDS
CTAGCTGGTCTTCTAGCTGG+TGG 0.579831 2.2:+22268269 MS.gene065561:CDS
GGTGGCATGGCCTTGCCAGA+TGG 0.588864 2.2:+22266271 None:intergenic
CTATCTACGACCCAAAGGAC+TGG 0.590400 2.2:+22267628 MS.gene065561:CDS
ACCTCCAGTGTAGACATCAC+CGG 0.624297 2.2:-22267658 None:intergenic
CAGCCCTATCTACGACCCAA+AGG 0.645785 2.2:+22267623 MS.gene065561:intron
CCAACCGGTGATGTCTACAC+TGG 0.647391 2.2:+22267654 MS.gene065561:CDS
AAGCACTTGTGTTGTAGACA+AGG 0.648013 2.2:-22268298 None:intergenic
GCCCAGATAGCCAAGCCAGT+CGG 0.648776 2.2:-22268239 None:intergenic
ACCGGTGATGTCTACACTGG+AGG 0.649993 2.2:+22267657 MS.gene065561:CDS
CCAGTGTAGACATCACCGGT+TGG 0.671824 2.2:-22267654 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTTTTATTTTATTT+TGG + chr2.2:22266328-22266347 MS.gene065561:intron 0.0%
!!! TAGTTTTAGATTATTTATTT+TGG + chr2.2:22266357-22266376 MS.gene065561:intron 10.0%
!!! AAATTACATTTTGAATAAGT+AGG - chr2.2:22267588-22267607 None:intergenic 15.0%
!!! AACTTAATAAACTTTTTTTG+AGG + chr2.2:22266426-22266445 MS.gene065561:intron 15.0%
!!! CAATTTTTTTTTGTATAAAG+TGG + chr2.2:22267976-22267995 MS.gene065561:intron 15.0%
!!! CTTAATTTCAATTTTATTCA+GGG + chr2.2:22267152-22267171 MS.gene065561:intron 15.0%
!!! TAACAAATTTTATTAATTGC+AGG + chr2.2:22268215-22268234 MS.gene065561:intron 15.0%
!!! TCTTAATTTCAATTTTATTC+AGG + chr2.2:22267151-22267170 MS.gene065561:intron 15.0%
!!! TTTTAGATTATTTATTTTGG+TGG + chr2.2:22266360-22266379 MS.gene065561:intron 15.0%
!! AATAATAGATAACTATGTTC+TGG - chr2.2:22267059-22267078 None:intergenic 20.0%
!! ACATGTTTAATAACATAGTT+AGG + chr2.2:22267274-22267293 MS.gene065561:intron 20.0%
!! ATTAAACATGTAAACAAAAC+TGG - chr2.2:22267265-22267284 None:intergenic 20.0%
!! ATTATCAAGATTTATTTCCA+TGG + chr2.2:22266532-22266551 MS.gene065561:intron 20.0%
!! GATCTAATGAAATACATTAA+AGG + chr2.2:22266955-22266974 MS.gene065561:intron 20.0%
!! TGAAATAATTATGTCATTAG+AGG + chr2.2:22267297-22267316 MS.gene065561:intron 20.0%
!! TTATCAAGATTTATTTCCAT+GGG + chr2.2:22266533-22266552 MS.gene065561:intron 20.0%
!!! ATTTATTCAACTTTTAGCTT+TGG + chr2.2:22266600-22266619 MS.gene065561:intron 20.0%
!!! ATTTTTTTGTTTTAATCCTC+TGG - chr2.2:22266563-22266582 None:intergenic 20.0%
!!! TAAAGCAATGTTTTAAAAAC+TGG + chr2.2:22267769-22267788 MS.gene065561:intron 20.0%
!!! TTTCATTTTTTCTGAATTCT+AGG + chr2.2:22267238-22267257 MS.gene065561:intron 20.0%
!!! TTTTTAAAACATTGCTTTAG+AGG - chr2.2:22267768-22267787 None:intergenic 20.0%
! AAAAACAATCATGACATAAG+TGG + chr2.2:22267334-22267353 MS.gene065561:intron 25.0%
! ATAAAATAGCATGAACTGTA+GGG + chr2.2:22267733-22267752 MS.gene065561:intron 25.0%
! ATGTAGAAAGTGATTCTTAA+GGG + chr2.2:22266772-22266791 MS.gene065561:intron 25.0%
! GATAACTAAAATCTGACTTA+TGG - chr2.2:22266495-22266514 None:intergenic 25.0%
! TAGGTCAAAGTCTATATAAT+TGG - chr2.2:22267569-22267588 None:intergenic 25.0%
! TATGTAGAAAGTGATTCTTA+AGG + chr2.2:22266771-22266790 MS.gene065561:intron 25.0%
!! GGAATTTTATGTAGTAACAT+AGG + chr2.2:22266381-22266400 MS.gene065561:intron 25.0%
!! TTCAATTTTATTCAGGGAAA+GGG + chr2.2:22267158-22267177 MS.gene065561:intron 25.0%
!! TTTCAATTTTATTCAGGGAA+AGG + chr2.2:22267157-22267176 MS.gene065561:intron 25.0%
!! TTTGTTATCATACTATTGCT+AGG - chr2.2:22268202-22268221 None:intergenic 25.0%
!!! AAGTAAGGTGGATTTTATAT+AGG + chr2.2:22266933-22266952 MS.gene065561:intron 25.0%
!!! AGGTAAGAAACTTCTTTTTT+TGG + chr2.2:22267677-22267696 MS.gene065561:intron 25.0%
!!! GGTAAGAAACTTCTTTTTTT+GGG + chr2.2:22267678-22267697 MS.gene065561:intron 25.0%
AAATCTTTGTATAGCCAACA+CGG - chr2.2:22268142-22268161 None:intergenic 30.0%
AACATCAAGTCATCAACAAT+AGG - chr2.2:22267898-22267917 None:intergenic 30.0%
AACCACATATGCTAATTGAA+AGG + chr2.2:22266728-22266747 MS.gene065561:intron 30.0%
ATCCTTTCAATTAGCATATG+TGG - chr2.2:22266733-22266752 None:intergenic 30.0%
ATGTTTATTTACTTCAGAGG+AGG + chr2.2:22267999-22268018 MS.gene065561:intron 30.0%
CAGAAAGCCCAAAAATATAA+GGG - chr2.2:22267089-22267108 None:intergenic 30.0%
GAATAAATAGTAGACCAAAG+AGG - chr2.2:22266626-22266645 None:intergenic 30.0%
GATAAAATAGCATGAACTGT+AGG + chr2.2:22267732-22267751 MS.gene065561:intron 30.0%
GCTGGTACATATAAAAAAGT+TGG + chr2.2:22267451-22267470 MS.gene065561:intron 30.0%
GTTTCTCACAAGATAAAGTA+AGG + chr2.2:22266918-22266937 MS.gene065561:intron 30.0%
TACCAAGTGTTTCATATAAG+AGG - chr2.2:22266662-22266681 None:intergenic 30.0%
TCAGAAAGCCCAAAAATATA+AGG - chr2.2:22267090-22267109 None:intergenic 30.0%
TGGATGTTTATTTACTTCAG+AGG + chr2.2:22267996-22268015 MS.gene065561:intron 30.0%
TGTTTATTTACTTCAGAGGA+GGG + chr2.2:22268000-22268019 MS.gene065561:intron 30.0%
! TGAGTGTCCCTTATATTTTT+GGG + chr2.2:22267079-22267098 MS.gene065561:intron 30.0%
! TTGAGTGTCCCTTATATTTT+TGG + chr2.2:22267078-22267097 MS.gene065561:intron 30.0%
! TTGATAATTCGTAGTTCGAT+CGG - chr2.2:22266520-22266539 None:intergenic 30.0%
!! TAGAGAGTAGTGTTTAGTTT+TGG + chr2.2:22268035-22268054 MS.gene065561:intron 30.0%
AGTGTTTCATATAAGAGGAG+AGG - chr2.2:22266657-22266676 None:intergenic 35.0%
ATAAGTTCAACTGGTCGAAT+TGG + chr2.2:22267801-22267820 MS.gene065561:intron 35.0%
ATAGGGCTGCATAATAAAAC+AGG - chr2.2:22267612-22267631 None:intergenic 35.0%
CAAGACTAATGTCTCATGTT+TGG + chr2.2:22267416-22267435 MS.gene065561:intron 35.0%
CATATGTGGTTGAGATGTTA+AGG - chr2.2:22266719-22266738 None:intergenic 35.0%
CTCCTCTTATATGAAACACT+TGG + chr2.2:22266657-22266676 MS.gene065561:intron 35.0%
GTGTTTCATATAAGAGGAGA+GGG - chr2.2:22266656-22266675 None:intergenic 35.0%
TAAGTTCAACTGGTCGAATT+GGG + chr2.2:22267802-22267821 MS.gene065561:intron 35.0%
TACCAGTTTGTGGACAAATA+CGG + chr2.2:22267185-22267204 MS.gene065561:CDS 35.0%
TATGTAGTAACATAGGCTTG+TGG + chr2.2:22266388-22266407 MS.gene065561:intron 35.0%
TCTCACAAGATAAAGTAAGG+TGG + chr2.2:22266921-22266940 MS.gene065561:intron 35.0%
! AAATACGGTGCTAATGTTGA+TGG + chr2.2:22267200-22267219 MS.gene065561:CDS 35.0%
! CATGAGCGAAGATAGTTTTT+AGG - chr2.2:22268066-22268085 None:intergenic 35.0%
! GAAACACTTGGTAGTTTCTA+AGG + chr2.2:22266669-22266688 MS.gene065561:intron 35.0%
!! CTTGGTAGTTTCTAAGGTAT+TGG + chr2.2:22266675-22266694 MS.gene065561:intron 35.0%
AAGCACTTGTGTTGTAGACA+AGG - chr2.2:22268301-22268320 None:intergenic 40.0%
AATTGGGAATCGTCTATCAC+TGG + chr2.2:22267818-22267837 MS.gene065561:intron 40.0%
ATCCAAAACATGAGAGACGT+TGG - chr2.2:22267520-22267539 None:intergenic 40.0%
ATCGAGCTGATAAGTTCAAC+TGG + chr2.2:22267792-22267811 MS.gene065561:intron 40.0%
CAACTATTTCAAGACCGTGT+TGG + chr2.2:22268125-22268144 MS.gene065561:intron 40.0%
GCATGAACTGTAGGGATTTA+TGG + chr2.2:22267741-22267760 MS.gene065561:intron 40.0%
GTTTGGATTGATACTCTAGC+TGG + chr2.2:22267433-22267452 MS.gene065561:intron 40.0%
TTATTAATTGCAGGTCCGAC+TGG + chr2.2:22268224-22268243 MS.gene065561:intron 40.0%
! GTGCTAATGTTGATGGATAC+AGG + chr2.2:22267207-22267226 MS.gene065561:CDS 40.0%
!! AAAGGGTGTTTACCAGTTTG+TGG + chr2.2:22267175-22267194 MS.gene065561:CDS 40.0%
!!! ACTTTTAGCTTTGGCCTCTT+TGG + chr2.2:22266609-22266628 MS.gene065561:intron 40.0%
AGTCCTTTGGGTCGTAGATA+GGG - chr2.2:22267629-22267648 None:intergenic 45.0%
ATGGAAAAGATGCTTCTGGC+AGG + chr2.2:22266290-22266309 MS.gene065561:CDS 45.0%
CACCGTATTTGTCCACAAAC+TGG - chr2.2:22267190-22267209 None:intergenic 45.0%
CCAGATGGAAAAGATGCTTC+TGG + chr2.2:22266286-22266305 MS.gene065561:CDS 45.0%
TATTTCCATGGGAACACCAG+AGG + chr2.2:22266544-22266563 MS.gene065561:intron 45.0%
TGCTTCTGGCAGGAAACAAA+AGG + chr2.2:22266300-22266319 MS.gene065561:CDS 45.0%
TTAATCCTCTGGTGTTCCCA+TGG - chr2.2:22266552-22266571 None:intergenic 45.0%
! AGCATCTTTTCCATCTGGCA+AGG - chr2.2:22266284-22266303 None:intergenic 45.0%
! GACCAACGTCTCTCATGTTT+TGG + chr2.2:22267515-22267534 MS.gene065561:intron 45.0%
! GGAATCGTCTATCACTGGTT+CGG + chr2.2:22267823-22267842 MS.gene065561:intron 45.0%
!!! CCAGAAGCATCTTTTCCATC+TGG - chr2.2:22266289-22266308 None:intergenic 45.0%
ACCTCCAGTGTAGACATCAC+CGG - chr2.2:22267661-22267680 None:intergenic 50.0%
ATCTGGGCAGTAACTCTAGC+TGG + chr2.2:22268254-22268273 MS.gene065561:CDS 50.0%
CAGTCCTTTGGGTCGTAGAT+AGG - chr2.2:22267630-22267649 None:intergenic 50.0%
CTATCTACGACCCAAAGGAC+TGG + chr2.2:22267628-22267647 MS.gene065561:CDS 50.0%
!! ACTCTAGCTGGTCTTCTAGC+TGG + chr2.2:22268266-22268285 MS.gene065561:CDS 50.0%
ACCGGTGATGTCTACACTGG+AGG + chr2.2:22267657-22267676 MS.gene065561:CDS 55.0%
CAGCCCTATCTACGACCCAA+AGG + chr2.2:22267623-22267642 MS.gene065561:intron 55.0%
CCAACCGGTGATGTCTACAC+TGG + chr2.2:22267654-22267673 MS.gene065561:CDS 55.0%
CCAGTGTAGACATCACCGGT+TGG - chr2.2:22267657-22267676 None:intergenic 55.0%
CGGTTGGAGACCAGTCCTTT+GGG - chr2.2:22267641-22267660 None:intergenic 55.0%
! AATTGCAGGTCCGACTGGCT+TGG + chr2.2:22268229-22268248 MS.gene065561:intron 55.0%
!! CCAAAGGACTGGTCTCCAAC+CGG + chr2.2:22267639-22267658 MS.gene065561:CDS 55.0%
!! CTAGCTGGTCTTCTAGCTGG+TGG + chr2.2:22268269-22268288 MS.gene065561:CDS 55.0%
!! TCCGACTGGCTTGGCTATCT+GGG + chr2.2:22268238-22268257 MS.gene065561:CDS 55.0%
CCGGTTGGAGACCAGTCCTT+TGG - chr2.2:22267642-22267661 None:intergenic 60.0%
GCCCAGATAGCCAAGCCAGT+CGG - chr2.2:22268242-22268261 None:intergenic 60.0%
!! GTCCGACTGGCTTGGCTATC+TGG + chr2.2:22268237-22268256 MS.gene065561:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 22266277 22268331 22266277 ID=MS.gene065561
chr2.2 mRNA 22266277 22268331 22266277 ID=MS.gene065561.t1;Parent=MS.gene065561
chr2.2 exon 22266277 22266321 22266277 ID=MS.gene065561.t1.exon1;Parent=MS.gene065561.t1
chr2.2 CDS 22266277 22266321 22266277 ID=cds.MS.gene065561.t1;Parent=MS.gene065561.t1
chr2.2 exon 22267173 22267228 22267173 ID=MS.gene065561.t1.exon2;Parent=MS.gene065561.t1
chr2.2 CDS 22267173 22267228 22267173 ID=cds.MS.gene065561.t1;Parent=MS.gene065561.t1
chr2.2 exon 22267626 22267678 22267626 ID=MS.gene065561.t1.exon3;Parent=MS.gene065561.t1
chr2.2 CDS 22267626 22267678 22267626 ID=cds.MS.gene065561.t1;Parent=MS.gene065561.t1
chr2.2 exon 22268237 22268331 22268237 ID=MS.gene065561.t1.exon4;Parent=MS.gene065561.t1
chr2.2 CDS 22268237 22268331 22268237 ID=cds.MS.gene065561.t1;Parent=MS.gene065561.t1
Gene Sequence

>MS.gene065561

ATGGCCTTGCCAGATGGAAAAGATGCTTCTGGCAGGAAACAAAAGGTGTGTTTAATTTTTTTATTTTATTTTGGTGCATATAGTTTTAGATTATTTATTTTGGTGGAATTTTATGTAGTAACATAGGCTTGTGGTTGATTCAAGTATTTAACTTAATAAACTTTTTTTGAGGTTCAATTGTACTAGAAATCTAAGTTCAGATATGATTGAAATAACCATAAGTCAGATTTTAGTTATCTTCCGATCGAACTACGAATTATCAAGATTTATTTCCATGGGAACACCAGAGGATTAAAACAAAAAAATTATTAACTGATATAGTTATTTATTCAACTTTTAGCTTTGGCCTCTTTGGTCTACTATTTATTCTTTATAACCCTCTCCTCTTATATGAAACACTTGGTAGTTTCTAAGGTATTGGAAAATATATCACTATAGTCCTTAACATCTCAACCACATATGCTAATTGAAAGGATTTCACATGATTCAGAGATTATGTAGAAAGTGATTCTTAAGGGATTAAGTTGAAGATTTATTGTTTAATTTAAACATTTATTTATTTGTAGTAAATACATCTTATGAATATGCATACTTTTTTTTTTTACACAAATTATTTTTGTAGTGATATTCAAACATATAAAGTTTCTCACAAGATAAAGTAAGGTGGATTTTATATAGGATCTAATGAAATACATTAAAGGAAAAGAAAATATTAATTAACTTAACTTTCATGTTTATGATAACTAAAATATTGTTATCTTTTTTGTTTTTTGAACAAGCCAGAACATAGTTATCTATTATTTGAGTGTCCCTTATATTTTTGGGCTTTCTGAATTGTTGATTTATAGTTTTCATTTCATCTTATCTTCTAACTTCTTAATTTCAATTTTATTCAGGGAAAGGGTGTTTACCAGTTTGTGGACAAATACGGTGCTAATGTTGATGGATACAGGTAACTTACTTTCATTTTTTCTGAATTCTAGGTCCAGTTTTGTTTACATGTTTAATAACATAGTTAGGTGAAATAATTATGTCATTAGAGGAACTAATCTCAATTAAAAACAATCATGACATAAGTGGTTAAAATTATAATAAATTTAAATCAAACTATTATGATCATTCATGCATTATTATGTAATCAAGACTAATGTCTCATGTTTGGATTGATACTCTAGCTGGTACATATAAAAAAGTTGGAATAAGAGATCAAGTTCAATTTAATTAAGTAATGTAATCAAGACCAACGTCTCTCATGTTTTGGATATTGATTTTAAATTAATTATTTTCATCCAATTATATAGACTTTGACCTACTTATTCAAAATGTAATTTTCCTGTTTTATTATGCAGCCCTATCTACGACCCAAAGGACTGGTCTCCAACCGGTGATGTCTACACTGGAGGTAAGAAACTTCTTTTTTTGGGTAACTTTGATGTCTTAAAATTGACTCTACAAGATAAAATAGCATGAACTGTAGGGATTTATGGTCCTCTAAAGCAATGTTTTAAAAACTGGATCGAGCTGATAAGTTCAACTGGTCGAATTGGGAATCGTCTATCACTGGTTCGGAACTGAGCAATTCGCTTTAAACGATAAAATAAGTAAATTGATGAACATACCTATTGTTGATGACTTGATGTTATAATGTGATCATGAATGTGTCAGAATATCAACTATTGATTTTTTTCATTAAATGATTCAATTTTTTTTTGTATAAAGTGGATGTTTATTTACTTCAGAGGAGGGAAATCAAAATCTTAGAGAGTAGTGTTTAGTTTTGGTTCAACCTAAAAACTATCTTCGCTCATGAATGTGTTAGAATATCAACTGTTTTGAGAGTCATGTTATCAACTATTTCAAGACCGTGTTGGCTATACAAAGATTTTCTTGATCAAATTCAAATGTTAAAGAACAAATTCTTTCCTAGCAATAGTATGATAACAAATTTTATTAATTGCAGGTCCGACTGGCTTGGCTATCTGGGCAGTAACTCTAGCTGGTCTTCTAGCTGGTGGTGCACTCCTTGTCTACAACACAAGTGCTTTGTCACAATAG

Protein sequence

>MS.gene065561.t1

MALPDGKDASGRKQKGKGVYQFVDKYGANVDGYSPIYDPKDWSPTGDVYTGGPTGLAIWAVTLAGLLAGGALLVYNTSALSQ