AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594610 MtrunA17_Chr4g0011001 98.294 293 5 0 1 293 1 293 0.0 600
Msa0594610 MtrunA17_Chr7g0276121 51.667 300 138 3 11 303 7 306 2.12e-90 271
Msa0594610 MtrunA17_Chr4g0009911 32.083 240 141 5 54 282 45 273 6.15e-34 125
Msa0594610 MtrunA17_Chr1g0199411 30.682 264 153 5 28 279 12 257 1.11e-27 108
Msa0594610 MtrunA17_Chr1g0201631 27.365 296 189 9 24 301 30 317 1.38e-27 110
Msa0594610 MtrunA17_Chr6g0464751 29.893 281 153 12 49 303 58 320 3.74e-25 104
Msa0594610 MtrunA17_Chr3g0131931 31.188 202 114 6 85 268 103 297 1.66e-22 96.7
Msa0594610 MtrunA17_Chr6g0462021 29.537 281 156 11 13 270 37 298 1.68e-22 97.1
Msa0594610 MtrunA17_Chr7g0251761 29.044 272 165 9 13 268 37 296 3.97e-18 84.3
Msa0594610 MtrunA17_Chr7g0255161 27.083 240 125 10 64 267 15 240 9.84e-17 79.7
Msa0594610 MtrunA17_Chr7g0255151 27.670 206 112 7 85 267 111 302 2.57e-16 79.0
Msa0594610 MtrunA17_Chr7g0273371 25.670 261 143 8 25 258 79 315 1.49e-15 76.6
Msa0594610 MtrunA17_Chr1g0163561 31.351 185 96 6 3 185 56 211 2.50e-15 75.5
Msa0594610 MtrunA17_Chr4g0037991 29.515 227 120 10 20 233 88 287 8.31e-15 73.9
Msa0594610 MtrunA17_Chr4g0049351 29.018 224 130 9 85 292 325 535 1.89e-14 73.6
Msa0594610 MtrunA17_Chr4g0064931 27.000 300 174 13 21 293 281 562 2.13e-14 73.6
Msa0594610 MtrunA17_Chr1g0211241 26.214 309 167 13 15 300 363 633 6.00e-14 72.4
Msa0594610 MtrunA17_Chr1g0211231 26.603 312 160 15 6 304 288 543 2.81e-12 67.0
Msa0594610 MtrunA17_Chr1g0193941 27.389 157 100 4 45 198 84 229 5.15e-12 66.2
Msa0594610 MtrunA17_Chr5g0406311 29.947 187 110 7 120 300 163 334 5.13e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594610 AT3G60800.1 84.039 307 47 1 1 305 1 307 0.0 521
Msa0594610 AT4G22750.1 66.000 300 97 3 8 305 1 297 9.99e-131 374
Msa0594610 AT4G00840.1 54.730 296 124 4 11 303 2 290 1.33e-104 307
Msa0594610 AT4G00840.2 55.769 208 82 4 11 215 2 202 1.32e-62 197
Msa0594610 AT5G04270.1 32.624 282 149 8 18 283 2 258 1.23e-32 122
Msa0594610 AT3G09320.1 34.307 274 150 11 20 279 4 261 2.95e-32 121
Msa0594610 AT3G26935.1 28.470 281 170 9 24 288 44 309 3.24e-25 105
Msa0594610 AT4G24630.1 28.464 267 167 8 24 273 29 288 8.15e-25 103
Msa0594610 AT5G41060.2 27.007 274 166 10 13 268 21 278 5.34e-23 98.2
Msa0594610 AT5G41060.1 27.007 274 166 10 13 268 36 293 7.03e-23 98.2
Msa0594610 AT5G41060.3 28.384 229 134 8 57 268 11 226 3.57e-22 95.1
Msa0594610 AT5G41060.4 28.384 229 134 8 57 268 11 226 3.57e-22 95.1
Msa0594610 AT5G50020.3 27.341 267 169 8 24 273 29 287 2.46e-21 93.6
Msa0594610 AT5G50020.2 26.667 270 167 9 24 273 59 317 3.14e-21 93.6
Msa0594610 AT5G05070.1 28.070 228 122 8 85 288 118 327 6.58e-18 84.0
Msa0594610 AT3G56920.1 27.542 236 123 10 66 268 64 284 5.84e-17 80.5
Msa0594610 AT3G04970.1 27.059 255 135 9 25 255 79 306 5.93e-17 80.9
Msa0594610 AT3G48760.1 27.317 205 124 6 85 271 110 307 1.59e-16 80.1
Msa0594610 AT5G50020.1 26.217 267 165 9 24 273 29 280 1.98e-16 79.7
Msa0594610 AT3G04970.3 27.642 246 127 9 34 255 1 219 4.16e-16 77.8
Msa0594610 AT2G40990.1 32.278 158 83 6 123 268 158 303 5.07e-16 78.2
Msa0594610 AT3G04970.2 27.016 248 130 8 25 245 79 302 1.31e-15 76.3
Msa0594610 AT5G20350.1 25.649 308 185 13 21 304 276 563 1.42e-15 77.4
Msa0594610 AT3G56930.1 26.601 203 126 5 85 268 94 292 1.83e-15 77.0
Msa0594610 AT3G18620.1 54.386 57 26 0 126 182 149 205 3.50e-15 75.5
Msa0594610 AT2G14255.1 31.343 201 115 8 72 260 314 503 3.81e-15 76.3
Msa0594610 AT4G01730.1 27.513 189 118 4 67 242 105 287 1.02e-12 68.9
Msa0594610 AT3G51390.1 32.143 140 89 3 127 266 164 297 8.86e-11 62.4
Msa0594610 AT3G51390.2 32.143 140 89 3 127 266 159 292 8.99e-11 62.4

Find 115 sgRNAs with CRISPR-Local

Find 378 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAGGGTTTATTGAGAATT+TGG 0.109054 4_2:+22668936 None:intergenic
ATCCGGCTTGTAAGTAATTC+TGG 0.174428 4_2:-22662853 Msa0594610:three_prime_UTR
CCAAGATCATAACGCCATTT+TGG 0.205394 4_2:+22664647 None:intergenic
CTAGGGTTTATTGAGAATTT+GGG 0.240991 4_2:+22668937 None:intergenic
GAACAAATTCTAACATTATA+AGG 0.261084 4_2:+22662689 None:intergenic
CAGTTTGGAGGTACACTTCC+AGG 0.285240 4_2:+22666186 None:intergenic
TTAAACCAAATATGCCCTTA+TGG 0.302319 4_2:+22663162 None:intergenic
TGATTCTCTTATTGCTGTCT+TGG 0.311178 4_2:-22668584 Msa0594610:CDS
TTTGATCTTGTTTCATGCTT+TGG 0.318169 4_2:-22668560 Msa0594610:intron
TATGTTCCTTCTTTGTATAA+TGG 0.318990 4_2:-22668613 Msa0594610:CDS
CCTCTCTCCTCATCAATTGT+AGG 0.323601 4_2:+22666159 None:intergenic
CCAAAATGGCGTTATGATCT+TGG 0.333453 4_2:-22664647 Msa0594610:CDS
GTCATGGGTTTGGTGAATCT+AGG 0.337349 4_2:+22668919 None:intergenic
TACCGGATCCGTTGATTAGA+TGG 0.344373 4_2:+22666087 None:intergenic
ATACAAAGAAGGAACATAAT+TGG 0.344556 4_2:+22668618 None:intergenic
ATTGAGATCGAAGTGGGTTT+TGG 0.345179 4_2:-22668786 Msa0594610:five_prime_UTR
GGAAGAGAATTGCAATGCTT+TGG 0.353783 4_2:+22662666 None:intergenic
GCAAGAGAGCTAGGTGTTCC+AGG 0.359082 4_2:+22665493 None:intergenic
TCTGATTATGCACATATCAT+TGG 0.372598 4_2:-22665338 Msa0594610:CDS
TGTGGGTAGTTAACTGTGTT+GGG 0.376839 4_2:-22665882 Msa0594610:CDS
AGAAGAAAACTACTCCAAAA+TGG 0.382057 4_2:-22664661 Msa0594610:CDS
TTGTTGGTTCTTGGTGTTGT+TGG 0.390832 4_2:-22668667 Msa0594610:CDS
GAAGAGAATTGCAATGCTTT+GGG 0.393702 4_2:+22662667 None:intergenic
TGTATGAGTGAGAAAGTAAA+GGG 0.400983 4_2:+22662877 None:intergenic
GGAGAGGAAGATCCATTAGT+TGG 0.403198 4_2:-22666138 Msa0594610:CDS
CTTTCTCACTCATACAAATC+CGG 0.412453 4_2:-22662870 Msa0594610:three_prime_UTR
GGCATCGAGGCGGCTTGAGC+TGG 0.413050 4_2:+22666049 None:intergenic
AGCTCCGAGGTTGTTCTTGT+CGG 0.414652 4_2:-22668879 Msa0594610:five_prime_UTR
TGATTGATTATGCATAGATC+TGG 0.415700 4_2:-22668757 Msa0594610:exon
TCATGGGTTTGGTGAATCTA+GGG 0.417197 4_2:+22668920 None:intergenic
CGCTTTCGCATTGAGTGTGA+TGG 0.421207 4_2:-22665365 Msa0594610:CDS
AATTGATGATTCTGCTTTGT+GGG 0.422280 4_2:-22668843 Msa0594610:five_prime_UTR
TCAATTGTAGGCCTCCAGTT+TGG 0.424125 4_2:+22666171 None:intergenic
ACCTGAAACAGAGTGAAAAC+CGG 0.429843 4_2:+22663091 None:intergenic
TTCATTGACTAACAACTCAT+TGG 0.431744 4_2:+22662541 None:intergenic
CTGGAAGTGTACCTCCAAAC+TGG 0.434261 4_2:-22666185 Msa0594610:CDS
ATGATGTGTACACTTCTGTT+TGG 0.440503 4_2:+22662927 None:intergenic
TTCCTTCTTTGTATAATGGA+GGG 0.446114 4_2:-22668609 Msa0594610:CDS
CTGAACTCTAACCCAACTAA+TGG 0.447036 4_2:+22666126 None:intergenic
GTGCATGTGTTAAGTTGAAA+TGG 0.449179 4_2:-22662771 Msa0594610:three_prime_UTR
GTGTGGGTAGTTAACTGTGT+TGG 0.451518 4_2:-22665883 Msa0594610:CDS
GTTCCTTCTTTGTATAATGG+AGG 0.453444 4_2:-22668610 Msa0594610:CDS
TCCGGCTTGTAAGTAATTCT+GGG 0.456621 4_2:-22662852 Msa0594610:three_prime_UTR
AAATTGATGATTCTGCTTTG+TGG 0.456748 4_2:-22668844 Msa0594610:five_prime_UTR
TTCATAGCATTCTTTAGTGA+TGG 0.458599 4_2:-22665523 Msa0594610:CDS
GGAAAACTTTACCTCAATAG+TGG 0.459164 4_2:+22665300 None:intergenic
AGCTATTGGGAATCGAAGTC+GGG 0.460014 4_2:+22663191 None:intergenic
TTGGGACGGACAAGAAATAC+TGG 0.473728 4_2:-22663286 Msa0594610:CDS
CAGCTATTGGGAATCGAAGT+CGG 0.479426 4_2:+22663190 None:intergenic
CCTGAAACAGAGTGAAAACC+GGG 0.485054 4_2:+22663092 None:intergenic
GAGAGGAAGATCCATTAGTT+GGG 0.491844 4_2:-22666137 Msa0594610:CDS
AAATGGAGGGAGCAATGAGA+GGG 0.494826 4_2:-22662754 Msa0594610:three_prime_UTR
TGTACACATCATGTAACAAA+AGG 0.494958 4_2:-22662916 Msa0594610:three_prime_UTR
AAAGTTGTAGCAAGAGAGCT+AGG 0.504375 4_2:+22665484 None:intergenic
TTGTATGAGTGAGAAAGTAA+AGG 0.504793 4_2:+22662876 None:intergenic
TACTCGAGACCTTGTAGAGC+TGG 0.507547 4_2:+22663224 None:intergenic
TGCACAGCTTTACGAGGACT+TGG 0.507888 4_2:-22668703 Msa0594610:CDS
CGAAGGATGCCAGCTCTACA+AGG 0.508944 4_2:-22663233 Msa0594610:CDS
ATCCATCTAATCAACGGATC+CGG 0.513865 4_2:-22666089 Msa0594610:CDS
GTTTGTGCAGATGAATTGCT+TGG 0.517980 4_2:+22662827 None:intergenic
GCTTTCGCATTGAGTGTGAT+GGG 0.523751 4_2:-22665364 Msa0594610:CDS
ATATCTTCTTCTGAATAAGC+AGG 0.525822 4_2:+22663257 None:intergenic
AACCCTCCATTATACAAAGA+AGG 0.526029 4_2:+22668607 None:intergenic
GAAATGGAGGGAGCAATGAG+AGG 0.526481 4_2:-22662755 Msa0594610:three_prime_UTR
ACTCACAAACTGAACAATGA+TGG 0.526589 4_2:+22666028 None:intergenic
TTTAGTGATGGAGAAATCCC+TGG 0.528395 4_2:-22665511 Msa0594610:CDS
CTTTGCATTGAGATCGAAGT+GGG 0.532329 4_2:-22668792 Msa0594610:five_prime_UTR
ATTGTAGGCCTCCAGTTTGG+AGG 0.536225 4_2:+22666174 None:intergenic
TTTATTTGCAAAACTGTCCC+CGG 0.537247 4_2:-22663110 Msa0594610:three_prime_UTR
GCAGTAACGATGCAGTCACT+AGG 0.540451 4_2:+22665551 None:intergenic
TGGGCGATGTGTGCTGAAGA+TGG 0.549501 4_2:-22665917 Msa0594610:CDS
GCCCAGAATTACTTACAAGC+CGG 0.551718 4_2:+22662851 None:intergenic
TGCAGTTGGTTATGCTATTG+TGG 0.552325 4_2:-22666236 Msa0594610:intron
ATCAACGGATCCGGTATTGT+AGG 0.557479 4_2:-22666080 Msa0594610:CDS
GGTTGCACTTCCTACAATAC+CGG 0.558949 4_2:+22666070 None:intergenic
ATGGAGGGAGCAATGAGAGG+GGG 0.560870 4_2:-22662752 Msa0594610:three_prime_UTR
AATTGAATAGAAGAAAATGA+AGG 0.563143 4_2:+22662645 None:intergenic
TGCTAATACAACCACTATTG+AGG 0.565179 4_2:-22665311 Msa0594610:intron
AACAGGAGACAGAATTAACA+TGG 0.565999 4_2:-22663015 Msa0594610:three_prime_UTR
GTGTTCATACCAAGAAAAGA+AGG 0.566591 4_2:+22665839 None:intergenic
AATGGAGGGAGCAATGAGAG+GGG 0.568316 4_2:-22662753 Msa0594610:three_prime_UTR
CAAGAGAGCTAGGTGTTCCA+GGG 0.573246 4_2:+22665494 None:intergenic
GATCTGGAGCTACGATGGCA+TGG 0.585026 4_2:-22668741 Msa0594610:CDS
CAGTCACTAGGGACGTCTCA+AGG 0.590525 4_2:+22665563 None:intergenic
TAGGGTTTATTGAGAATTTG+GGG 0.592322 4_2:+22668938 None:intergenic
GTGTGAGGCTATGGTTTGAG+AGG 0.595746 4_2:-22662727 Msa0594610:three_prime_UTR
GCATAGATCTGGAGCTACGA+TGG 0.600274 4_2:-22668746 Msa0594610:CDS
GGCGTTATGATCTTGGTCGT+CGG 0.603365 4_2:-22664640 Msa0594610:CDS
AGTGGAGAAGAAGATTGAAG+TGG 0.604930 4_2:+22668975 None:intergenic
AAGTTCTGCACAGCTTTACG+AGG 0.607927 4_2:-22668709 Msa0594610:CDS
ATTCAGAAGAAGATATTCGA+AGG 0.610112 4_2:-22663250 Msa0594610:CDS
CATGTGTTAAGTTGAAATGG+AGG 0.616401 4_2:-22662768 Msa0594610:three_prime_UTR
CAGTAACGATGCAGTCACTA+GGG 0.618039 4_2:+22665552 None:intergenic
AATTGATGAGGAGAGAGGAG+AGG 0.619514 4_2:-22666154 Msa0594610:CDS
AACACCGACAAGAACAACCT+CGG 0.620932 4_2:+22668875 None:intergenic
AGTTTGGAGGTACACTTCCA+GGG 0.622562 4_2:+22666187 None:intergenic
CTAAAGAATGCTATGAAATG+CGG 0.626695 4_2:+22665529 None:intergenic
CCTACAATTGATGAGGAGAG+AGG 0.629217 4_2:-22666159 Msa0594610:CDS
GTGGGTAGTTAACTGTGTTG+GGG 0.629727 4_2:-22665881 Msa0594610:CDS
ATTGATGATTCTGCTTTGTG+GGG 0.631990 4_2:-22668842 Msa0594610:five_prime_UTR
CTGGAGGCCTACAATTGATG+AGG 0.643127 4_2:-22666166 Msa0594610:CDS
ACAGGAGACAGAATTAACAT+GGG 0.644781 4_2:-22663014 Msa0594610:three_prime_UTR
AGAGTGATCCATCTAATCAA+CGG 0.644934 4_2:-22666095 Msa0594610:CDS
TCACACTCAATGCGAAAGCG+AGG 0.648642 4_2:+22665368 None:intergenic
AGATGGACCACCATTGTGTG+TGG 0.658837 4_2:-22665900 Msa0594610:CDS
AGTTAACTACCCACACACAA+TGG 0.666889 4_2:+22665890 None:intergenic
GAAGTGTACCTCCAAACTGG+AGG 0.685237 4_2:-22666182 Msa0594610:CDS
GAACAATGATGGCATCGAGG+CGG 0.692188 4_2:+22666039 None:intergenic
TAACTACCCACACACAATGG+TGG 0.692891 4_2:+22665893 None:intergenic
ATGTGTTAAGTTGAAATGGA+GGG 0.701506 4_2:-22662767 Msa0594610:three_prime_UTR
CAGGAGACAGAATTAACATG+GGG 0.710211 4_2:-22663013 Msa0594610:three_prime_UTR
GATGGACCACCATTGTGTGT+GGG 0.716633 4_2:-22665899 Msa0594610:CDS
ACTTTGCATTGAGATCGAAG+TGG 0.716971 4_2:-22668793 Msa0594610:five_prime_UTR
ACTGAACAATGATGGCATCG+AGG 0.726860 4_2:+22666036 None:intergenic
CTGAAACAGAGTGAAAACCG+GGG 0.785419 4_2:+22663093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTATATTATTAATTA+AGG + chr4_2:22664452-22664471 None:intergenic 0.0%
!!! TAAATTTCTTGTTTTATTTA+TGG - chr4_2:22665953-22665972 Msa0594610:intron 10.0%
!!! TGAATTTTGTTTGAAATTAA+TGG - chr4_2:22663279-22663298 Msa0594610:CDS 15.0%
!!! AACCAAGTATTTTTTTTTAT+TGG + chr4_2:22665981-22666000 None:intergenic 15.0%
!! CATGAAAAATATTTGATAAA+TGG - chr4_2:22667090-22667109 Msa0594610:intron 15.0%
!!! ATAATTTCATTCAGATTTTT+AGG - chr4_2:22667917-22667936 Msa0594610:intron 15.0%
!!! CATATTGATTTTTGATATAT+GGG - chr4_2:22668023-22668042 Msa0594610:intron 15.0%
!! TATAACATATAAGAATATAC+AGG + chr4_2:22668158-22668177 None:intergenic 15.0%
!!! TTAAATGTTTTTGAAATAGT+TGG - chr4_2:22668230-22668249 Msa0594610:intron 15.0%
!! ATCATAAAAAAAATCATACT+AGG + chr4_2:22668801-22668820 None:intergenic 15.0%
!!! AGAAAATATTTTACAAATAC+TGG + chr4_2:22669004-22669023 None:intergenic 15.0%
!!! ATTTGAATTTGTTAGTGTTT+TGG - chr4_2:22663092-22663111 Msa0594610:three_prime_UTR 20.0%
!! ATAATTAGCGTAAAATAATG+AGG - chr4_2:22663687-22663706 Msa0594610:intron 20.0%
!! AAAAGAACAATTAGTTATCA+CGG - chr4_2:22663812-22663831 Msa0594610:intron 20.0%
!! AGAAAAAGCTAAAATGAAAT+GGG + chr4_2:22663974-22663993 None:intergenic 20.0%
!! AAGAAAAAGCTAAAATGAAA+TGG + chr4_2:22663975-22663994 None:intergenic 20.0%
!!! TCATTTTGACTGTAAAAAAA+AGG + chr4_2:22664258-22664277 None:intergenic 20.0%
!!! AACTTGATTTTATTCCTTTT+CGG - chr4_2:22665651-22665670 Msa0594610:intron 20.0%
!! CATTCATAAAAGTAATAACT+GGG + chr4_2:22665814-22665833 None:intergenic 20.0%
!! TCATTCATAAAAGTAATAAC+TGG + chr4_2:22665815-22665834 None:intergenic 20.0%
!! TGCCAATAAAAAAAAATACT+TGG - chr4_2:22665976-22665995 Msa0594610:intron 20.0%
!! TACAAAAAAGTTCATTTGTA+TGG + chr4_2:22666327-22666346 None:intergenic 20.0%
!!! ATGAACTTTTTTGTATTACT+TGG - chr4_2:22666332-22666351 Msa0594610:intron 20.0%
!!! TGAACTTTTTTGTATTACTT+GGG - chr4_2:22666333-22666352 Msa0594610:intron 20.0%
!! AACAATTTAAACTCAACTTA+GGG + chr4_2:22666714-22666733 None:intergenic 20.0%
!! TATATGTTCTCTAATATGTA+TGG - chr4_2:22667048-22667067 Msa0594610:intron 20.0%
!! ATATGTTCTCTAATATGTAT+GGG - chr4_2:22667049-22667068 Msa0594610:intron 20.0%
!!! TCAAATATTTTTCATGAAGT+TGG + chr4_2:22667086-22667105 None:intergenic 20.0%
!!! GCATATTGATTTTTGATATA+TGG - chr4_2:22668022-22668041 Msa0594610:intron 20.0%
!!! ACTTTATATATCTAGGATAT+GGG + chr4_2:22668181-22668200 None:intergenic 20.0%
!!! TACTTTATATATCTAGGATA+TGG + chr4_2:22668182-22668201 None:intergenic 20.0%
!! TGTTTGTACTTTATATATCT+AGG + chr4_2:22668188-22668207 None:intergenic 20.0%
!! CAACATAAATCATTATGTAA+GGG + chr4_2:22668278-22668297 None:intergenic 20.0%
!!! TTTTTTATGATGTTACTTAG+AGG - chr4_2:22668810-22668829 Msa0594610:five_prime_UTR 20.0%
!! GAACAAATTCTAACATTATA+AGG + chr4_2:22668917-22668936 None:intergenic 20.0%
!! AATTGAATAGAAGAAAATGA+AGG + chr4_2:22668961-22668980 None:intergenic 20.0%
!!! AATTTGGGGATTTTGATTTT+TGG + chr4_2:22662654-22662673 None:intergenic 25.0%
!!! AATTTTTTTGATTGGGTCAT+GGG + chr4_2:22662702-22662721 None:intergenic 25.0%
!!! GAGCTAGAATTTTTTTGATT+GGG + chr4_2:22662709-22662728 None:intergenic 25.0%
!!! ATTATGATTTTGTTGGTTCT+TGG - chr4_2:22662927-22662946 Msa0594610:three_prime_UTR 25.0%
! ATACAAAGAAGGAACATAAT+TGG + chr4_2:22662988-22663007 None:intergenic 25.0%
! TATGTTCCTTCTTTGTATAA+TGG - chr4_2:22662990-22663009 Msa0594610:three_prime_UTR 25.0%
!!! ATCTGTTTTTGATTTGTTTG+AGG - chr4_2:22663132-22663151 Msa0594610:three_prime_UTR 25.0%
! ATTTATAGGGTCTATTTGAT+GGG - chr4_2:22663219-22663238 Msa0594610:CDS 25.0%
! CAACTAATAAAAACTTCCAA+CGG + chr4_2:22663320-22663339 None:intergenic 25.0%
!! GTTGGAAGTTTTTATTAGTT+GGG - chr4_2:22663319-22663338 Msa0594610:intron 25.0%
!! TGATCTTGGTAACAAAATAA+TGG - chr4_2:22663916-22663935 Msa0594610:intron 25.0%
!!! TCTTTAAAGTATTCATTAGC+TGG - chr4_2:22663945-22663964 Msa0594610:intron 25.0%
!!! CTTTAAAGTATTCATTAGCT+GGG - chr4_2:22663946-22663965 Msa0594610:intron 25.0%
!! ATGTAGAATAATGAAGTGTT+GGG - chr4_2:22664593-22664612 Msa0594610:intron 25.0%
!!! TGAGACTAATTGTCATTTTT+CGG + chr4_2:22664641-22664660 None:intergenic 25.0%
!! AAAAATGACAATTAGTCTCA+AGG - chr4_2:22664641-22664660 Msa0594610:CDS 25.0%
! AGGTGTTAAATATCTAATTG+AGG - chr4_2:22664705-22664724 Msa0594610:intron 25.0%
!!! AAAGACTGTTTTTTAAACCT+TGG - chr4_2:22665096-22665115 Msa0594610:intron 25.0%
! AAATGTGACACTACAAAAAA+CGG + chr4_2:22665621-22665640 None:intergenic 25.0%
! CGTTATAGTAATACTCATAT+TGG + chr4_2:22665924-22665943 None:intergenic 25.0%
! CAACAATTTAAACTCAACTT+AGG + chr4_2:22666715-22666734 None:intergenic 25.0%
! TATGTTCTCTAATATGTATG+GGG - chr4_2:22667050-22667069 Msa0594610:intron 25.0%
! TGTAACAAACTTTCATTCAA+TGG + chr4_2:22667370-22667389 None:intergenic 25.0%
!!! AGTTTGTTACAAAATCTTAG+AGG - chr4_2:22667379-22667398 Msa0594610:intron 25.0%
!! AACTGTTATGAATTCAGTTT+TGG - chr4_2:22667454-22667473 Msa0594610:intron 25.0%
! ATCTACAAATTATGACATCT+TGG + chr4_2:22667573-22667592 None:intergenic 25.0%
! ATTAGCTACTTGGATTATTT+AGG + chr4_2:22667603-22667622 None:intergenic 25.0%
!!! TCATCATGCAATTTTATTTG+TGG - chr4_2:22667793-22667812 Msa0594610:intron 25.0%
!!! TTTTATTTGTGGAAAGACAA+TGG - chr4_2:22667804-22667823 Msa0594610:intron 25.0%
! AACTAAAAACTAGTTCATGT+AGG + chr4_2:22667838-22667857 None:intergenic 25.0%
!! AACCATATTTTATGTTTCCA+TGG - chr4_2:22668055-22668074 Msa0594610:intron 25.0%
! TTCCATGGAAACATAAAATA+TGG + chr4_2:22668060-22668079 None:intergenic 25.0%
!!! AAATATTCCTACTTTTTCCA+TGG + chr4_2:22668075-22668094 None:intergenic 25.0%
! GCAACATAAATCATTATGTA+AGG + chr4_2:22668279-22668298 None:intergenic 25.0%
!! TTCTTTGATATCAGGTTTTT+GGG - chr4_2:22668299-22668318 Msa0594610:intron 25.0%
!! GTGTACAAATCTTTAAAAAC+AGG - chr4_2:22668571-22668590 Msa0594610:CDS 25.0%
TAGGGTTTATTGAGAATTTG+GGG + chr4_2:22662668-22662687 None:intergenic 30.0%
CTAGGGTTTATTGAGAATTT+GGG + chr4_2:22662669-22662688 None:intergenic 30.0%
! TCTAGGGTTTATTGAGAATT+TGG + chr4_2:22662670-22662689 None:intergenic 30.0%
!!! GAATTTTTTTGATTGGGTCA+TGG + chr4_2:22662703-22662722 None:intergenic 30.0%
!!! GGAGCTAGAATTTTTTTGAT+TGG + chr4_2:22662710-22662729 None:intergenic 30.0%
! AAATTGATGATTCTGCTTTG+TGG - chr4_2:22662759-22662778 Msa0594610:three_prime_UTR 30.0%
! AATTGATGATTCTGCTTTGT+GGG - chr4_2:22662760-22662779 Msa0594610:three_prime_UTR 30.0%
TGATTGATTATGCATAGATC+TGG - chr4_2:22662846-22662865 Msa0594610:three_prime_UTR 30.0%
! TGGTTCGATTATGATTTTGT+TGG - chr4_2:22662920-22662939 Msa0594610:three_prime_UTR 30.0%
! TTCCTTCTTTGTATAATGGA+GGG - chr4_2:22662994-22663013 Msa0594610:three_prime_UTR 30.0%
! TTTGATCTTGTTTCATGCTT+TGG - chr4_2:22663043-22663062 Msa0594610:three_prime_UTR 30.0%
!! TGATTTGTTTGAGGATGTTT+AGG - chr4_2:22663141-22663160 Msa0594610:three_prime_UTR 30.0%
!! AGAATTTAGCTTGTTGACTT+AGG - chr4_2:22663186-22663205 Msa0594610:exon 30.0%
!! GAATTTAGCTTGTTGACTTA+GGG - chr4_2:22663187-22663206 Msa0594610:exon 30.0%
TAGGGATCATGATGATTTAT+AGG - chr4_2:22663205-22663224 Msa0594610:CDS 30.0%
AGGGATCATGATGATTTATA+GGG - chr4_2:22663206-22663225 Msa0594610:CDS 30.0%
GATTTATAGGGTCTATTTGA+TGG - chr4_2:22663218-22663237 Msa0594610:CDS 30.0%
! CGTTGGAAGTTTTTATTAGT+TGG - chr4_2:22663318-22663337 Msa0594610:intron 30.0%
CAGCAAAAGCTAAAATCAAA+TGG + chr4_2:22663348-22663367 None:intergenic 30.0%
!!! TTGATTTTAGCTTTTGCTGT+CGG - chr4_2:22663349-22663368 Msa0594610:intron 30.0%
TCTTGGAAGTTATCATTAGT+TGG - chr4_2:22663615-22663634 Msa0594610:intron 30.0%
CTTGGAAGTTATCATTAGTT+GGG - chr4_2:22663616-22663635 Msa0594610:intron 30.0%
GTGTTAGATATCTACATTGA+TGG - chr4_2:22663709-22663728 Msa0594610:intron 30.0%
TTAGATATCTACATTGATGG+TGG - chr4_2:22663712-22663731 Msa0594610:intron 30.0%
ACAGTCAAAATGAAGAAGAA+AGG - chr4_2:22664265-22664284 Msa0594610:intron 30.0%
CAGTCAAAATGAAGAAGAAA+GGG - chr4_2:22664266-22664285 Msa0594610:intron 30.0%
GAAAGATTGATTAGAGATCT+AGG - chr4_2:22664290-22664309 Msa0594610:intron 30.0%
GATGTAGAATAATGAAGTGT+TGG - chr4_2:22664592-22664611 Msa0594610:intron 30.0%
TGATGTTTATTCGAAAAACG+AGG - chr4_2:22664685-22664704 Msa0594610:intron 30.0%
AAAAGAACAAGTATCTCTGA+CGG - chr4_2:22664740-22664759 Msa0594610:intron 30.0%
AATGCAACTTTAAACCTTGA+CGG + chr4_2:22664872-22664891 None:intergenic 30.0%
!!! ACAGACTGTTTTTTAAACCT+TGG - chr4_2:22665138-22665157 Msa0594610:intron 30.0%
!!! GATTTTTAAAACAACTGCCA+AGG + chr4_2:22665158-22665177 None:intergenic 30.0%
!!! GTTTTAAAAATCCGAGTTAC+TGG - chr4_2:22665166-22665185 Msa0594610:intron 30.0%
AAACTTCAGAAGAAACACTT+AGG + chr4_2:22665217-22665236 None:intergenic 30.0%
TTAATGATGCGTTGTTTATC+TGG - chr4_2:22665298-22665317 Msa0594610:intron 30.0%
! CAAGTATTTCCTTCTTTTCT+TGG - chr4_2:22665755-22665774 Msa0594610:intron 30.0%
!! TAATTATTTCTGACTTGCGA+TGG - chr4_2:22665840-22665859 Msa0594610:intron 30.0%
! TCGTTTTACTTTAATGTCTC+AGG + chr4_2:22665888-22665907 None:intergenic 30.0%
!! GTAATACTCATATTGGTTCT+TGG + chr4_2:22665917-22665936 None:intergenic 30.0%
CTAAAGAATGCTATGAAATG+CGG + chr4_2:22666077-22666096 None:intergenic 30.0%
TTCATAGCATTCTTTAGTGA+TGG - chr4_2:22666080-22666099 Msa0594610:CDS 30.0%
GATGGAATACAAATCTAGAA+TGG + chr4_2:22666173-22666192 None:intergenic 30.0%
TTGTTACATAAACTCACAGA+TGG + chr4_2:22666191-22666210 None:intergenic 30.0%
GTGAGTTTATGTAACAATGT+TGG - chr4_2:22666194-22666213 Msa0594610:CDS 30.0%
TCTGATTATGCACATATCAT+TGG - chr4_2:22666265-22666284 Msa0594610:intron 30.0%
ACTTATATGATAAGCACTTG+TGG + chr4_2:22666472-22666491 None:intergenic 30.0%
! ATATGCTTTTCCATGTTGTA+TGG - chr4_2:22666856-22666875 Msa0594610:intron 30.0%
AGAAGAAAACTACTCCAAAA+TGG - chr4_2:22666942-22666961 Msa0594610:intron 30.0%
CTAGATTGTTGACACATAAT+GGG + chr4_2:22667137-22667156 None:intergenic 30.0%
AGTAACTTAAGTATGCATTG+TGG - chr4_2:22667203-22667222 Msa0594610:intron 30.0%
GTAACTTAAGTATGCATTGT+GGG - chr4_2:22667204-22667223 Msa0594610:intron 30.0%
GAAATCTAATAAAAAGCAGC+AGG + chr4_2:22667269-22667288 None:intergenic 30.0%
GGAAGAGAACTAGATATTAT+TGG - chr4_2:22668000-22668019 Msa0594610:intron 30.0%
TATGTTTCCATGGAAAAAGT+AGG - chr4_2:22668065-22668084 Msa0594610:intron 30.0%
! TATGTTGCTTCTTTGATATC+AGG - chr4_2:22668291-22668310 Msa0594610:intron 30.0%
! CTTCTTTGATATCAGGTTTT+TGG - chr4_2:22668298-22668317 Msa0594610:intron 30.0%
ATATCTTCTTCTGAATAAGC+AGG + chr4_2:22668349-22668368 None:intergenic 30.0%
ATTCAGAAGAAGATATTCGA+AGG - chr4_2:22668353-22668372 Msa0594610:intron 30.0%
!! CTTATGGTTTTTCAGCTATT+GGG + chr4_2:22668428-22668447 None:intergenic 30.0%
CAATAGCTGAAAAACCATAA+GGG - chr4_2:22668427-22668446 Msa0594610:intron 30.0%
! AAAAACCATAAGGGCATATT+TGG - chr4_2:22668436-22668455 Msa0594610:intron 30.0%
TTAAACCAAATATGCCCTTA+TGG + chr4_2:22668444-22668463 None:intergenic 30.0%
TGTACACATCATGTAACAAA+AGG - chr4_2:22668687-22668706 Msa0594610:CDS 30.0%
TGTATGAGTGAGAAAGTAAA+GGG + chr4_2:22668729-22668748 None:intergenic 30.0%
TTGTATGAGTGAGAAAGTAA+AGG + chr4_2:22668730-22668749 None:intergenic 30.0%
!! ATGTGTTAAGTTGAAATGGA+GGG - chr4_2:22668836-22668855 Msa0594610:five_prime_UTR 30.0%
! TTCATTGACTAACAACTCAT+TGG + chr4_2:22669065-22669084 None:intergenic 30.0%
!! TCAAAAAAATTCTAGCTCCG+AGG - chr4_2:22662711-22662730 Msa0594610:three_prime_UTR 35.0%
! ATTGATGATTCTGCTTTGTG+GGG - chr4_2:22662761-22662780 Msa0594610:three_prime_UTR 35.0%
GTTCCTTCTTTGTATAATGG+AGG - chr4_2:22662993-22663012 Msa0594610:three_prime_UTR 35.0%
AACCCTCCATTATACAAAGA+AGG + chr4_2:22662999-22663018 None:intergenic 35.0%
TGATTCTCTTATTGCTGTCT+TGG - chr4_2:22663019-22663038 Msa0594610:three_prime_UTR 35.0%
GAGAATAATGATTGTTCCGT+TGG - chr4_2:22663301-22663320 Msa0594610:intron 35.0%
AAAGTGAGAAGTAGTTGACA+AGG - chr4_2:22663429-22663448 Msa0594610:intron 35.0%
AAAAACGACAAGTAGTTGTC+AGG - chr4_2:22663493-22663512 Msa0594610:intron 35.0%
AAAACGACAAGTAGTTGTCA+GGG - chr4_2:22663494-22663513 Msa0594610:intron 35.0%
! CTCGATCTTGGTAACAATAA+TGG - chr4_2:22663586-22663605 Msa0594610:intron 35.0%
AACAATAATGGTTGCTCTCT+TGG - chr4_2:22663598-22663617 Msa0594610:intron 35.0%
! TTTTTGCCAGATTGTGAATG+CGG - chr4_2:22663658-22663677 Msa0594610:intron 35.0%
! AGAACAGAGATGAGATTTTG+TGG + chr4_2:22664224-22664243 None:intergenic 35.0%
CAGAATCATAGTTGTCAAAG+CGG + chr4_2:22664567-22664586 None:intergenic 35.0%
TTGGGCATCTTAATTGATGA+TGG - chr4_2:22664611-22664630 Msa0594610:intron 35.0%
AAAAAGACAAGTAGTCGTCA+AGG - chr4_2:22664936-22664955 Msa0594610:intron 35.0%
ACATAAAAAACCGTGACATC+TGG - chr4_2:22665059-22665078 Msa0594610:intron 35.0%
TAGTGATTAAACAACGACCA+AGG + chr4_2:22665116-22665135 None:intergenic 35.0%
AGAAACATCTTCCAGTAACT+CGG + chr4_2:22665180-22665199 None:intergenic 35.0%
TTTCCTCTTTATCATGCAGT+TGG - chr4_2:22665353-22665372 Msa0594610:CDS 35.0%
TAACCAACTGCATGATAAAG+AGG + chr4_2:22665359-22665378 None:intergenic 35.0%
TCCAGGGTCAATAAAAACTA+CGG + chr4_2:22665403-22665422 None:intergenic 35.0%
AGAGTGATCCATCTAATCAA+CGG - chr4_2:22665508-22665527 Msa0594610:CDS 35.0%
ACTCACAAACTGAACAATGA+TGG + chr4_2:22665578-22665597 None:intergenic 35.0%
!! TTCCTTTTCGGTTTGATATC+AGG - chr4_2:22665663-22665682 Msa0594610:intron 35.0%
!! TTTTCGGTTTGATATCAGGT+GGG - chr4_2:22665667-22665686 Msa0594610:intron 35.0%
GTGTTCATACCAAGAAAAGA+AGG + chr4_2:22665767-22665786 None:intergenic 35.0%
ACTTTAATGTCTCAGGAAGT+CGG + chr4_2:22665881-22665900 None:intergenic 35.0%
! GCTACAACTTTTCTTGCTTT+TGG - chr4_2:22666131-22666150 Msa0594610:CDS 35.0%
!! CAACTTTTCTTGCTTTTGGT+AGG - chr4_2:22666135-22666154 Msa0594610:CDS 35.0%
TGCTAATACAACCACTATTG+AGG - chr4_2:22666292-22666311 Msa0594610:intron 35.0%
GGAAAACTTTACCTCAATAG+TGG + chr4_2:22666306-22666325 None:intergenic 35.0%
TGAAAGTTGGATAAGGTGTA+GGG + chr4_2:22666369-22666388 None:intergenic 35.0%
CTAAATACTGCCATTCAACA+AGG + chr4_2:22666412-22666431 None:intergenic 35.0%
CTTGTTGAATGGCAGTATTT+AGG - chr4_2:22666410-22666429 Msa0594610:intron 35.0%
TGTACGTTGTTTATGCAAAC+AGG + chr4_2:22666437-22666456 None:intergenic 35.0%
TAAGCTATCTAAGAGAGCTT+AGG + chr4_2:22666567-22666586 None:intergenic 35.0%
AAGCTCTCTTAGATAGCTTA+TGG - chr4_2:22666567-22666586 Msa0594610:intron 35.0%
AAGCAAAAGTCCATACAACA+TGG + chr4_2:22666869-22666888 None:intergenic 35.0%
! TCGTCGGAAAAATTTTGAAC+AGG - chr4_2:22666979-22666998 Msa0594610:intron 35.0%
CCTAGATTGTTGACACATAA+TGG + chr4_2:22667138-22667157 None:intergenic 35.0%
CCATTATGTGTCAACAATCT+AGG - chr4_2:22667135-22667154 Msa0594610:intron 35.0%
AATCTAGGCACTAATGACTA+AGG - chr4_2:22667150-22667169 Msa0594610:intron 35.0%
ATAGCAATCCTAAGCTAACT+AGG - chr4_2:22667415-22667434 Msa0594610:intron 35.0%
!!! TTCAGTTTTGGACAGTTACA+AGG - chr4_2:22667466-22667485 Msa0594610:intron 35.0%
TTCAGCAAGTATTAGCTACT+TGG + chr4_2:22667613-22667632 None:intergenic 35.0%
TGACACAGAAACATGTCATT+CGG + chr4_2:22667749-22667768 None:intergenic 35.0%
! CATGATGATGTATTAGACAC+TGG + chr4_2:22667781-22667800 None:intergenic 35.0%
TACAGCACAAGTTTACACAT+GGG + chr4_2:22667889-22667908 None:intergenic 35.0%
ATACAGCACAAGTTTACACA+TGG + chr4_2:22667890-22667909 None:intergenic 35.0%
!! TTGATATCAGGTTTTTGGGA+CGG - chr4_2:22668303-22668322 Msa0594610:intron 35.0%
!! CCTTATGGTTTTTCAGCTAT+TGG + chr4_2:22668429-22668448 None:intergenic 35.0%
CCAATAGCTGAAAAACCATA+AGG - chr4_2:22668426-22668445 Msa0594610:intron 35.0%
TTTATTTGCAAAACTGTCCC+CGG - chr4_2:22668493-22668512 Msa0594610:intron 35.0%
AACAGGAGACAGAATTAACA+TGG - chr4_2:22668588-22668607 Msa0594610:CDS 35.0%
ACAGGAGACAGAATTAACAT+GGG - chr4_2:22668589-22668608 Msa0594610:CDS 35.0%
ATGATGTGTACACTTCTGTT+TGG + chr4_2:22668679-22668698 None:intergenic 35.0%
CTTTCTCACTCATACAAATC+CGG - chr4_2:22668733-22668752 Msa0594610:CDS 35.0%
!! GTGCATGTGTTAAGTTGAAA+TGG - chr4_2:22668832-22668851 Msa0594610:five_prime_UTR 35.0%
!! CATGTGTTAAGTTGAAATGG+AGG - chr4_2:22668835-22668854 Msa0594610:five_prime_UTR 35.0%
GAAGAGAATTGCAATGCTTT+GGG + chr4_2:22668939-22668958 None:intergenic 35.0%
AGTGGAGAAGAAGATTGAAG+TGG + chr4_2:22662631-22662650 None:intergenic 40.0%
!!! GGGGATTTTGATTTTTGGAG+TGG + chr4_2:22662649-22662668 None:intergenic 40.0%
!! TCATGGGTTTGGTGAATCTA+GGG + chr4_2:22662686-22662705 None:intergenic 40.0%
!! TTTTGATTGGGTCATGGGTT+TGG + chr4_2:22662697-22662716 None:intergenic 40.0%
ACTTTGCATTGAGATCGAAG+TGG - chr4_2:22662810-22662829 Msa0594610:three_prime_UTR 40.0%
CTTTGCATTGAGATCGAAGT+GGG - chr4_2:22662811-22662830 Msa0594610:three_prime_UTR 40.0%
!! ATTGAGATCGAAGTGGGTTT+TGG - chr4_2:22662817-22662836 Msa0594610:three_prime_UTR 40.0%
!! TTGTTGGTTCTTGGTGTTGT+TGG - chr4_2:22662936-22662955 Msa0594610:three_prime_UTR 40.0%
TGGGTTTGAGTGTGATACTA+TGG - chr4_2:22663238-22663257 Msa0594610:CDS 40.0%
!! TTAGCTTTTGCTGTCGGATT+TGG - chr4_2:22663355-22663374 Msa0594610:intron 40.0%
! AAAAGTGCGAAGTAGTTGTC+AGG - chr4_2:22663742-22663761 Msa0594610:intron 40.0%
AAAGTGCGAAGTAGTTGTCA+GGG - chr4_2:22663743-22663762 Msa0594610:intron 40.0%
! TTCCACAAAATCAAGCATCG+TGG + chr4_2:22663867-22663886 None:intergenic 40.0%
! GGATGATTTTGTCGTGATCT+TGG - chr4_2:22663902-22663921 Msa0594610:intron 40.0%
TGAAGACGATCTAGAGATCT+AGG - chr4_2:22664330-22664349 Msa0594610:intron 40.0%
AAAATGACAAGTAGCCGTCA+AGG - chr4_2:22664855-22664874 Msa0594610:intron 40.0%
AACATCTGTATGAGCTTGTG+TGG + chr4_2:22665242-22665261 None:intergenic 40.0%
TGCAGTTGGTTATGCTATTG+TGG - chr4_2:22665367-22665386 Msa0594610:CDS 40.0%
! TCCGTAGTTTTTATTGACCC+TGG - chr4_2:22665399-22665418 Msa0594610:intron 40.0%
GAGAGGAAGATCCATTAGTT+GGG - chr4_2:22665466-22665485 Msa0594610:intron 40.0%
CTGAACTCTAACCCAACTAA+TGG + chr4_2:22665480-22665499 None:intergenic 40.0%
ATCCATCTAATCAACGGATC+CGG - chr4_2:22665514-22665533 Msa0594610:CDS 40.0%
CACCTGATATCAAACCGAAA+AGG + chr4_2:22665668-22665687 None:intergenic 40.0%
!! CTTTTCGGTTTGATATCAGG+TGG - chr4_2:22665666-22665685 Msa0594610:intron 40.0%
AGTTAACTACCCACACACAA+TGG + chr4_2:22665716-22665735 None:intergenic 40.0%
TGTGGGTAGTTAACTGTGTT+GGG - chr4_2:22665721-22665740 Msa0594610:intron 40.0%
! TTTAGTGATGGAGAAATCCC+TGG - chr4_2:22666092-22666111 Msa0594610:CDS 40.0%
AAAGTTGTAGCAAGAGAGCT+AGG + chr4_2:22666122-22666141 None:intergenic 40.0%
GAAAGTTGGATAAGGTGTAG+GGG + chr4_2:22666368-22666387 None:intergenic 40.0%
GTGAAAGTTGGATAAGGTGT+AGG + chr4_2:22666370-22666389 None:intergenic 40.0%
CTTCAGGTGAAAGTTGGATA+AGG + chr4_2:22666376-22666395 None:intergenic 40.0%
CAGTTTCTTCAGGTGAAAGT+TGG + chr4_2:22666382-22666401 None:intergenic 40.0%
AGGAGCAAAACAGTTTCTTC+AGG + chr4_2:22666392-22666411 None:intergenic 40.0%
! CTGTTTTGCTCCTTGTTGAA+TGG - chr4_2:22666399-22666418 Msa0594610:intron 40.0%
AACTCAACTTAGGGACTGTT+TGG + chr4_2:22666705-22666724 None:intergenic 40.0%
AGGATGCATTTCACTAGTTC+AGG + chr4_2:22666762-22666781 None:intergenic 40.0%
AAATGCATCCTCTGTCTATG+AGG - chr4_2:22666771-22666790 Msa0594610:intron 40.0%
AGCATTTACCTCATAGACAG+AGG + chr4_2:22666782-22666801 None:intergenic 40.0%
! GGACTTTTGCTTATGTGAAG+TGG - chr4_2:22666877-22666896 Msa0594610:intron 40.0%
GTGAAGTGGATACTGATCAA+TGG - chr4_2:22666891-22666910 Msa0594610:intron 40.0%
! CCAAGATCATAACGCCATTT+TGG + chr4_2:22666959-22666978 None:intergenic 40.0%
CCAAAATGGCGTTATGATCT+TGG - chr4_2:22666956-22666975 Msa0594610:intron 40.0%
GCACTAATGACTAAGGAAAC+TGG - chr4_2:22667157-22667176 Msa0594610:intron 40.0%
GCATTGTGGGAAACAAAACT+TGG - chr4_2:22667217-22667236 Msa0594610:intron 40.0%
! TGACAGAACCTAGTTAGCTT+AGG + chr4_2:22667426-22667445 None:intergenic 40.0%
GAAATGTCCAGTGTATGTTG+AGG - chr4_2:22667630-22667649 Msa0594610:intron 40.0%
GAAACTTCCTCAACATACAC+TGG + chr4_2:22667640-22667659 None:intergenic 40.0%
! TTGTGTCACTGTCAACATCT+CGG + chr4_2:22667706-22667725 None:intergenic 40.0%
! ACATGTTTCTGTGTCAGCTT+AGG - chr4_2:22667753-22667772 Msa0594610:intron 40.0%
ACAGCACAAGTTTACACATG+GGG + chr4_2:22667888-22667907 None:intergenic 40.0%
TGACTCGATGTCTCTATGTT+AGG - chr4_2:22667979-22667998 Msa0594610:intron 40.0%
ACCTGAAACAGAGTGAAAAC+CGG + chr4_2:22668515-22668534 None:intergenic 40.0%
CAGGAGACAGAATTAACATG+GGG - chr4_2:22668590-22668609 Msa0594610:CDS 40.0%
! ATCCGGCTTGTAAGTAATTC+TGG - chr4_2:22668750-22668769 Msa0594610:CDS 40.0%
! TCCGGCTTGTAAGTAATTCT+GGG - chr4_2:22668751-22668770 Msa0594610:CDS 40.0%
GTTTGTGCAGATGAATTGCT+TGG + chr4_2:22668779-22668798 None:intergenic 40.0%
GGAAGAGAATTGCAATGCTT+TGG + chr4_2:22668940-22668959 None:intergenic 40.0%
!! GTCATGGGTTTGGTGAATCT+AGG + chr4_2:22662687-22662706 None:intergenic 45.0%
AACACCGACAAGAACAACCT+CGG + chr4_2:22662731-22662750 None:intergenic 45.0%
AAGTTCTGCACAGCTTTACG+AGG - chr4_2:22662894-22662913 Msa0594610:three_prime_UTR 45.0%
! TTGCTGTCGGATTTGGAATG+CGG - chr4_2:22663362-22663381 Msa0594610:intron 45.0%
TTGTCAGGGTAGTTGTGTCA+CGG - chr4_2:22663508-22663527 Msa0594610:intron 45.0%
TGTCACGATGCTTGACTTTG+TGG - chr4_2:22663534-22663553 Msa0594610:intron 45.0%
GTGGAAATTGCGATCAAGCA+CGG - chr4_2:22663553-22663572 Msa0594610:intron 45.0%
! GGATGACGTTTTCTCGATCT+TGG - chr4_2:22663574-22663593 Msa0594610:intron 45.0%
TTTACGCCGCATTCACAATC+TGG + chr4_2:22663667-22663686 None:intergenic 45.0%
! TGCCACGATGCTTGATTTTG+TGG - chr4_2:22663862-22663881 Msa0594610:intron 45.0%
CTGAGCTCTGTTGTCAATAG+CGG - chr4_2:22664002-22664021 Msa0594610:intron 45.0%
TTGTCAATAGCGGTCTAACG+CGG - chr4_2:22664012-22664031 Msa0594610:intron 45.0%
AGGCAAAAGCGGAACAAAAG+CGG - chr4_2:22664100-22664119 Msa0594610:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG + chr4_2:22664559-22664578 None:intergenic 45.0%
! CATTTGTGCGCGATGCTTTT+TGG - chr4_2:22664831-22664850 Msa0594610:intron 45.0%
GCAGAAGTTGCGATCAAACA+TGG - chr4_2:22665005-22665024 Msa0594610:intron 45.0%
GTGATTGTGTCCAGATGTCA+CGG + chr4_2:22665072-22665091 None:intergenic 45.0%
! AGTTTGGAGGTACACTTCCA+GGG + chr4_2:22665419-22665438 None:intergenic 45.0%
TCAATTGTAGGCCTCCAGTT+TGG + chr4_2:22665435-22665454 None:intergenic 45.0%
CCTCTCTCCTCATCAATTGT+AGG + chr4_2:22665447-22665466 None:intergenic 45.0%
CCTACAATTGATGAGGAGAG+AGG - chr4_2:22665444-22665463 Msa0594610:intron 45.0%
AATTGATGAGGAGAGAGGAG+AGG - chr4_2:22665449-22665468 Msa0594610:intron 45.0%
GGAGAGGAAGATCCATTAGT+TGG - chr4_2:22665465-22665484 Msa0594610:intron 45.0%
! TACCGGATCCGTTGATTAGA+TGG + chr4_2:22665519-22665538 None:intergenic 45.0%
ATCAACGGATCCGGTATTGT+AGG - chr4_2:22665523-22665542 Msa0594610:CDS 45.0%
GGTTGCACTTCCTACAATAC+CGG + chr4_2:22665536-22665555 None:intergenic 45.0%
ACTGAACAATGATGGCATCG+AGG + chr4_2:22665570-22665589 None:intergenic 45.0%
TAACTACCCACACACAATGG+TGG + chr4_2:22665713-22665732 None:intergenic 45.0%
GTGTGGGTAGTTAACTGTGT+TGG - chr4_2:22665720-22665739 Msa0594610:intron 45.0%
GTGGGTAGTTAACTGTGTTG+GGG - chr4_2:22665722-22665741 Msa0594610:intron 45.0%
CAGTAACGATGCAGTCACTA+GGG + chr4_2:22666054-22666073 None:intergenic 45.0%
AAAGCGAGGTTCAAAACTGC+AGG + chr4_2:22666224-22666243 None:intergenic 45.0%
GCTTTCGCATTGAGTGTGAT+GGG - chr4_2:22666239-22666258 Msa0594610:intron 45.0%
CAACTTAGGGACTGTTTGGA+TGG + chr4_2:22666701-22666720 None:intergenic 45.0%
! GTTTGCTCTTTGTTGTGAGC+AGG - chr4_2:22666913-22666932 Msa0594610:intron 45.0%
TTTACACATGGGGGAAACGA+GGG + chr4_2:22667878-22667897 None:intergenic 45.0%
CAGCACAAGTTTACACATGG+GGG + chr4_2:22667887-22667906 None:intergenic 45.0%
TCACATCTGCAGAAAGAGTC+TGG + chr4_2:22667943-22667962 None:intergenic 45.0%
TTGGGACGGACAAGAAATAC+TGG - chr4_2:22668317-22668336 Msa0594610:intron 45.0%
!! GAATCGAAGTCGGGTTTTGA+TGG + chr4_2:22668406-22668425 None:intergenic 45.0%
AGCTATTGGGAATCGAAGTC+GGG + chr4_2:22668415-22668434 None:intergenic 45.0%
CAGCTATTGGGAATCGAAGT+CGG + chr4_2:22668416-22668435 None:intergenic 45.0%
CTGAAACAGAGTGAAAACCG+GGG + chr4_2:22668513-22668532 None:intergenic 45.0%
CCTGAAACAGAGTGAAAACC+GGG + chr4_2:22668514-22668533 None:intergenic 45.0%
GCCCAGAATTACTTACAAGC+CGG + chr4_2:22668755-22668774 None:intergenic 45.0%
AAATGGAGGGAGCAATGAGA+GGG - chr4_2:22668849-22668868 Msa0594610:five_prime_UTR 45.0%
AGCTCCGAGGTTGTTCTTGT+CGG - chr4_2:22662724-22662743 Msa0594610:three_prime_UTR 50.0%
! GCATAGATCTGGAGCTACGA+TGG - chr4_2:22662857-22662876 Msa0594610:three_prime_UTR 50.0%
TGCACAGCTTTACGAGGACT+TGG - chr4_2:22662900-22662919 Msa0594610:three_prime_UTR 50.0%
GAATGCGGCGTGGAAATAAG+AGG - chr4_2:22663377-22663396 Msa0594610:intron 50.0%
CGGCGTGGAAATAAGAGGTT+AGG - chr4_2:22663382-22663401 Msa0594610:intron 50.0%
GTGGAAGTTGCGATCAAGCA+TGG - chr4_2:22663881-22663900 Msa0594610:intron 50.0%
GGCAAAAGCGGAACAAAAGC+GGG - chr4_2:22664101-22664120 Msa0594610:intron 50.0%
TGGATGATGCAGTCACGATG+CGG - chr4_2:22665025-22665044 Msa0594610:intron 50.0%
! CAGTTTGGAGGTACACTTCC+AGG + chr4_2:22665420-22665439 None:intergenic 50.0%
! CTGGAAGTGTACCTCCAAAC+TGG - chr4_2:22665418-22665437 Msa0594610:intron 50.0%
GAAGTGTACCTCCAAACTGG+AGG - chr4_2:22665421-22665440 Msa0594610:intron 50.0%
ATTGTAGGCCTCCAGTTTGG+AGG + chr4_2:22665432-22665451 None:intergenic 50.0%
CTGGAGGCCTACAATTGATG+AGG - chr4_2:22665437-22665456 Msa0594610:intron 50.0%
! GAACAATGATGGCATCGAGG+CGG + chr4_2:22665567-22665586 None:intergenic 50.0%
AGATGGACCACCATTGTGTG+TGG - chr4_2:22665703-22665722 Msa0594610:intron 50.0%
GATGGACCACCATTGTGTGT+GGG - chr4_2:22665704-22665723 Msa0594610:intron 50.0%
GCAGTAACGATGCAGTCACT+AGG + chr4_2:22666055-22666074 None:intergenic 50.0%
CAAGAGAGCTAGGTGTTCCA+GGG + chr4_2:22666112-22666131 None:intergenic 50.0%
TCACACTCAATGCGAAAGCG+AGG + chr4_2:22666238-22666257 None:intergenic 50.0%
CGCTTTCGCATTGAGTGTGA+TGG - chr4_2:22666238-22666257 Msa0594610:intron 50.0%
GGCGTTATGATCTTGGTCGT+CGG - chr4_2:22666963-22666982 Msa0594610:intron 50.0%
GTTTACACATGGGGGAAACG+AGG + chr4_2:22667879-22667898 None:intergenic 50.0%
TACTCGAGACCTTGTAGAGC+TGG + chr4_2:22668382-22668401 None:intergenic 50.0%
! CCCGGTTTTCACTCTGTTTC+AGG - chr4_2:22668511-22668530 Msa0594610:intron 50.0%
GAAATGGAGGGAGCAATGAG+AGG - chr4_2:22668848-22668867 Msa0594610:five_prime_UTR 50.0%
AATGGAGGGAGCAATGAGAG+GGG - chr4_2:22668850-22668869 Msa0594610:five_prime_UTR 50.0%
GTGTGAGGCTATGGTTTGAG+AGG - chr4_2:22668876-22668895 Msa0594610:five_prime_UTR 50.0%
! GATCTGGAGCTACGATGGCA+TGG - chr4_2:22662862-22662881 Msa0594610:three_prime_UTR 55.0%
! CTTTTGCCTAACGCGCGACT+CGG + chr4_2:22664089-22664108 None:intergenic 55.0%
AGTCGCGCGTTAGGCAAAAG+CGG - chr4_2:22664089-22664108 Msa0594610:intron 55.0%
GGAACAAAAGCGGGTCAACG+CGG - chr4_2:22664110-22664129 Msa0594610:intron 55.0%
GAACAAAAGCGGGTCAACGC+GGG - chr4_2:22664111-22664130 Msa0594610:intron 55.0%
GGGCAAAAAGCGGGTCAATG+CGG - chr4_2:22664131-22664150 Msa0594610:intron 55.0%
GGCAAAAAGCGGGTCAATGC+GGG - chr4_2:22664132-22664151 Msa0594610:intron 55.0%
! TACCGCGCCGCGTCAATTTT+TGG - chr4_2:22664518-22664537 Msa0594610:intron 55.0%
! ACCGCGCCGCGTCAATTTTT+GGG - chr4_2:22664519-22664538 Msa0594610:intron 55.0%
GGCCACCCCAAAAATTGACG+CGG + chr4_2:22664528-22664547 None:intergenic 55.0%
ATGCAGTCACGATGCGGTCA+TGG - chr4_2:22665031-22665050 Msa0594610:intron 55.0%
TGGGCGATGTGTGCTGAAGA+TGG - chr4_2:22665686-22665705 Msa0594610:intron 55.0%
CAGTCACTAGGGACGTCTCA+AGG + chr4_2:22666043-22666062 None:intergenic 55.0%
GCAAGAGAGCTAGGTGTTCC+AGG + chr4_2:22666113-22666132 None:intergenic 55.0%
!! CTCGGTGTCAGTGTCTCGTT+TGG + chr4_2:22667688-22667707 None:intergenic 55.0%
CGAAGGATGCCAGCTCTACA+AGG - chr4_2:22668370-22668389 Msa0594610:intron 55.0%
ATGGAGGGAGCAATGAGAGG+GGG - chr4_2:22668851-22668870 Msa0594610:five_prime_UTR 55.0%
! CAATGAGAGGGGGAAGTGTG+AGG - chr4_2:22668861-22668880 Msa0594610:five_prime_UTR 55.0%
! GTCGGATTTGGAATGCGGCG+TGG - chr4_2:22663367-22663386 Msa0594610:intron 60.0%
GGGTCAACGCGGGCAAAAAG+CGG - chr4_2:22664121-22664140 Msa0594610:intron 60.0%
GGTCAACGCGGGCAAAAAGC+GGG - chr4_2:22664122-22664141 Msa0594610:intron 60.0%
GGGTCAACGCGGGCAAAAAG+CGG - chr4_2:22664121-22664140 Msa0594610:intron 60.0%
GGTCAACGCGGGCAAAAAGC+GGG - chr4_2:22664122-22664141 Msa0594610:intron 60.0%
GGGCAAAAAGCGGGTCAACG+CGG - chr4_2:22664162-22664181 Msa0594610:intron 60.0%
GGCAAAAAGCGGGTCAACGC+GGG - chr4_2:22664163-22664182 Msa0594610:intron 60.0%
GGGTCAACGCGGGCAAAAAG+CGG - chr4_2:22664121-22664140 Msa0594610:intron 60.0%
GGTCAACGCGGGCAAAAAGC+GGG - chr4_2:22664122-22664141 Msa0594610:intron 60.0%
! GCGGGCGCTTTTACATGACG+CGG + chr4_2:22664486-22664505 None:intergenic 60.0%
AATTGACGCGGCGCGGTAAG+CGG + chr4_2:22664516-22664535 None:intergenic 60.0%
CCCCAAAAATTGACGCGGCG+CGG + chr4_2:22664523-22664542 None:intergenic 60.0%
! CCGCGCCGCGTCAATTTTTG+GGG - chr4_2:22664520-22664539 Msa0594610:intron 60.0%
!! CGCCGCGTCAATTTTTGGGG+TGG - chr4_2:22664523-22664542 Msa0594610:intron 60.0%
CAGTCACGATGCGGTCATGG+TGG - chr4_2:22665034-22665053 Msa0594610:intron 60.0%
ACACATGGGGGAAACGAGGG+TGG + chr4_2:22667875-22667894 None:intergenic 60.0%
! GAGGGGGAAGTGTGAGGCTA+TGG - chr4_2:22668867-22668886 Msa0594610:five_prime_UTR 60.0%
CGGGTCAATGCGGGTCAACG+CGG - chr4_2:22664141-22664160 Msa0594610:intron 65.0%
GGGTCAATGCGGGTCAACGC+GGG - chr4_2:22664142-22664161 Msa0594610:intron 65.0%
ATTGACGCGGCGCGGTAAGC+GGG + chr4_2:22664515-22664534 None:intergenic 65.0%
GTCAAAGCGGAAACGCGGCG+CGG + chr4_2:22664554-22664573 None:intergenic 65.0%
GGTCTAACGCGGCGCGATCG+CGG - chr4_2:22664023-22664042 Msa0594610:intron 70.0%
CGGTCGCGTTCTGACGCGCT+AGG + chr4_2:22664069-22664088 None:intergenic 70.0%
ACGCGACCGAGTCGCGCGTT+AGG - chr4_2:22664080-22664099 Msa0594610:intron 70.0%
! GGCATCGAGGCGGCTTGAGC+TGG + chr4_2:22665557-22665576 None:intergenic 70.0%
! GGCGCGATCGCGGCGCAGAG+CGG - chr4_2:22664033-22664052 Msa0594610:intron 80.0%
! GCGGTAAGCGGGCGCGACGC+GGG + chr4_2:22664504-22664523 None:intergenic 80.0%
! CGCGGTAAGCGGGCGCGACG+CGG + chr4_2:22664505-22664524 None:intergenic 80.0%
! AGCGGAAACGCGGCGCGGCG+CGG + chr4_2:22664549-22664568 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 22662527 22669098 22662527 ID=Msa0594610;Name=Msa0594610
chr4_2 mRNA 22662527 22669098 22662527 ID=Msa0594610-mRNA-1;Parent=Msa0594610;Name=Msa0594610-mRNA-1;_AED=0.07;_eAED=0.07;_QI=328|1|1|1|1|1|7|666|305
chr4_2 exon 22662527 22663312 22662527 ID=Msa0594610-mRNA-1:exon:6146;Parent=Msa0594610-mRNA-1
chr4_2 exon 22664625 22664690 22664625 ID=Msa0594610-mRNA-1:exon:6145;Parent=Msa0594610-mRNA-1
chr4_2 exon 22665312 22665397 22665312 ID=Msa0594610-mRNA-1:exon:6144;Parent=Msa0594610-mRNA-1
chr4_2 exon 22665473 22665596 22665473 ID=Msa0594610-mRNA-1:exon:6143;Parent=Msa0594610-mRNA-1
chr4_2 exon 22665849 22665940 22665849 ID=Msa0594610-mRNA-1:exon:6142;Parent=Msa0594610-mRNA-1
chr4_2 exon 22666034 22666253 22666034 ID=Msa0594610-mRNA-1:exon:6141;Parent=Msa0594610-mRNA-1
chr4_2 exon 22668561 22669098 22668561 ID=Msa0594610-mRNA-1:exon:6140;Parent=Msa0594610-mRNA-1
chr4_2 five_prime_UTR 22668771 22669098 22668771 ID=Msa0594610-mRNA-1:five_prime_utr;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22668561 22668770 22668561 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22666034 22666253 22666034 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22665849 22665940 22665849 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22665473 22665596 22665473 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22665312 22665397 22665312 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22664625 22664690 22664625 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 CDS 22663193 22663312 22663193 ID=Msa0594610-mRNA-1:cds;Parent=Msa0594610-mRNA-1
chr4_2 three_prime_UTR 22662527 22663192 22662527 ID=Msa0594610-mRNA-1:three_prime_utr;Parent=Msa0594610-mRNA-1
Gene Sequence

>Msa0594610

ATGCATAGATCTGGAGCTACGATGGCATGGAACGTGTTCAAGTTCTGCACAGCTTTACGAGGACTTGGTTCGATTATGATTTTGTTGGTTCTTGGTGTTGTTGGTGTTACTTATTATGCTGTTGTTATTACCAATTATGTTCCTTCTTTGTATAATGGAGGGTTTGATTCTCTTATTGCTGTCTTGGTTTTGATCTTGTTTCATGCTTTGTTGGTTATGCTATTGTGGAGTTATTTTTCCGTAGTTTTTATTGACCCTGGAAGTGTACCTCCAAACTGGAGGCCTACAATTGATGAGGAGAGAGGAGAGGAAGATCCATTAGTTGGGTTAGAGTTCAGTAACATGCAGAGTGATCCATCTAATCAACGGATCCGGTATTGTAGGAAGTGCAACCAGCTCAAGCCGCCTCGATGCCATCATTGTTCAGTTTGTGGGCGATGTGTGCTGAAGATGGACCACCATTGTGTGTGGGTAGTTAACTGTGTTGGGGCTCTAAATTACAAGTATTTCCTTCTTTTCTTGTTTTATACTTTCCTTGAGACGTCCCTAGTGACTGCATCGTTACTGCCGCATTTCATAGCATTCTTTAGTGATGGAGAAATCCCTGGAACACCTAGCTCTCTTGCTACAACTTTTCTTGCTTTTGTTTTGAACCTCGCTTTCGCATTGAGTGTGATGGGATTTCTGATTATGCACATATCATTGGTTGCTGCTAATACAACCACTATTGAGGCATATGAGAAGAAAACTACTCCAAAATGGCGTTATGATCTTGGTCGTCGGAAAAATTTTGAACAGGTTTTTGGGACGGACAAGAAATACTGGTTCATTCCTGCTTATTCAGAAGAAGATATTCGAAGGATGCCAGCTCTACAAGGTCTCGAGTATCCATCAAAACCCGACTTCGATTCCCAATAG
Protein sequence

>Msa0594610

MHRSGATMAWNVFKFCTALRGLGSIMILLVLGVVGVTYYAVVITNYVPSLYNGGFDSLIAVLVLILFHALLVMLLWSYFSVVFIDPGSVPPNWRPTIDEERGEEDPLVGLEFSNMQSDPSNQRIRYCRKCNQLKPPRCHHCSVCGRCVLKMDHHCVWVVNCVGALNYKYFLLFLFYTFLETSLVTASLLPHFIAFFSDGEIPGTPSSLATTFLAFVLNLAFALSVMGFLIMHISLVAANTTTIEAYEKKTTPKWRYDLGRRKNFEQVFGTDKKYWFIPAYSEEDIRRMPALQGLEYPSKPDFDSQ*