AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0646710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646710 MtrunA17_Chr4g0035101 90.833 360 16 2 1 359 1 344 0.0 667
Msa0646710 MtrunA17_Chr3g0144641 53.461 419 114 18 1 359 1 398 3.86e-123 360
Msa0646710 MtrunA17_Chr7g0234761 51.691 207 84 6 69 261 220 424 5.87e-53 182
Msa0646710 MtrunA17_Chr5g0421111 46.332 259 102 13 119 359 319 558 2.81e-49 174
Msa0646710 MtrunA17_Chr8g0339091 60.000 145 52 3 106 248 242 382 3.50e-46 164
Msa0646710 MtrunA17_Chr8g0365941 54.088 159 68 3 88 245 238 392 3.52e-46 163
Msa0646710 MtrunA17_Chr1g0152131 45.972 211 72 6 138 334 226 408 6.42e-45 159
Msa0646710 MtrunA17_Chr8g0363921 75.258 97 24 0 155 251 148 244 3.81e-43 150
Msa0646710 MtrunA17_Chr5g0415291 59.701 134 42 2 114 247 79 200 9.63e-43 148
Msa0646710 MtrunA17_Chr7g0258161 74.194 93 24 0 151 243 166 258 6.62e-42 149
Msa0646710 MtrunA17_Chr1g0174161 62.712 118 35 3 137 250 143 255 2.39e-41 147
Msa0646710 MtrunA17_Chr8g0385911 82.143 84 14 1 155 237 142 225 1.11e-39 143
Msa0646710 MtrunA17_Chr3g0131771 75.000 92 20 1 155 243 144 235 2.10e-39 142
Msa0646710 MtrunA17_Chr6g0484271 63.636 99 35 1 138 235 134 232 1.87e-33 125
Msa0646710 MtrunA17_Chr4g0074161 71.212 66 18 1 158 223 206 270 1.41e-21 95.1
Msa0646710 MtrunA17_Chr1g0190731 68.182 66 20 1 158 223 193 257 1.63e-20 91.3
Msa0646710 MtrunA17_Chr4g0032951 56.923 65 28 0 158 222 355 419 4.49e-15 76.3
Msa0646710 MtrunA17_Chr1g0182791 52.174 69 32 1 160 228 319 386 7.98e-15 75.5
Msa0646710 MtrunA17_Chr4g0014771 53.846 65 30 0 158 222 118 182 1.41e-14 71.6
Msa0646710 MtrunA17_Chr2g0329041 52.308 65 31 0 156 220 330 394 2.46e-14 73.6
Msa0646710 MtrunA17_Chr1g0193321 45.205 73 39 1 160 232 464 535 2.58e-14 74.3
Msa0646710 MtrunA17_Chr1g0174271 48.718 78 38 1 151 226 289 366 2.78e-14 73.6
Msa0646710 MtrunA17_Chr1g0152651 45.455 88 43 2 135 222 255 337 7.88e-14 72.0
Msa0646710 MtrunA17_Chr7g0258181 45.783 83 43 1 151 231 234 316 8.35e-14 71.6
Msa0646710 MtrunA17_Chr1g0184841 63.636 66 23 1 158 223 120 184 9.09e-14 70.9
Msa0646710 MtrunA17_Chr1g0153241 40.909 88 50 2 138 224 64 150 1.36e-12 67.4
Msa0646710 MtrunA17_Chr3g0087771 49.231 65 33 0 158 222 322 386 2.73e-12 67.4
Msa0646710 MtrunA17_Chr7g0271251 34.706 170 99 7 124 286 396 560 3.50e-12 67.8
Msa0646710 MtrunA17_Chr8g0351881 47.692 65 34 0 158 222 316 380 5.27e-12 66.6
Msa0646710 MtrunA17_Chr7g0262691 37.097 124 57 5 108 223 206 316 1.24e-11 65.9
Msa0646710 MtrunA17_Chr3g0095391 36.893 103 60 3 160 259 311 411 2.27e-11 64.7
Msa0646710 MtrunA17_Chr5g0393721 34.146 123 63 3 100 222 183 287 3.50e-11 63.5
Msa0646710 MtrunA17_Chr3g0134741 51.562 64 30 1 159 222 230 292 4.31e-11 63.5
Msa0646710 MtrunA17_Chr7g0270871 43.939 66 36 1 169 234 213 277 5.23e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0646710 AT4G34530.1 39.118 363 139 11 12 329 5 330 4.07e-59 194
Msa0646710 AT2G18300.1 59.627 161 55 5 89 248 124 275 3.80e-52 176
Msa0646710 AT2G18300.2 60.897 156 51 5 89 243 124 270 3.65e-51 174
Msa0646710 AT1G68920.3 39.869 306 136 8 75 358 205 484 4.95e-51 177
Msa0646710 AT1G68920.1 41.297 293 124 7 79 358 228 485 6.55e-51 177
Msa0646710 AT1G68920.4 41.297 293 124 7 79 358 228 485 6.55e-51 177
Msa0646710 AT1G68920.2 41.297 293 124 7 79 358 228 485 6.55e-51 177
Msa0646710 AT2G18300.3 60.897 156 51 5 89 243 124 270 1.03e-50 173
Msa0646710 AT3G07340.2 53.093 194 70 4 78 271 195 367 1.75e-50 174
Msa0646710 AT3G07340.1 53.093 194 70 4 78 271 195 367 1.89e-50 175
Msa0646710 AT3G23690.1 56.742 178 67 4 106 281 140 309 4.35e-49 169
Msa0646710 AT1G26260.3 57.325 157 56 1 101 257 126 271 1.56e-48 167
Msa0646710 AT5G48560.2 49.495 198 94 3 54 245 192 389 2.50e-48 169
Msa0646710 AT1G26260.5 57.325 157 56 1 101 257 155 300 4.45e-48 167
Msa0646710 AT5G48560.1 49.495 198 94 3 54 245 192 389 5.09e-48 169
Msa0646710 AT1G26260.4 57.325 157 56 1 101 257 177 322 7.19e-48 166
Msa0646710 AT1G26260.1 57.325 157 56 1 101 257 177 322 7.19e-48 166
Msa0646710 AT1G26260.6 57.325 157 56 1 101 257 177 322 7.19e-48 166
Msa0646710 AT1G26260.2 57.325 157 56 1 101 257 177 322 7.19e-48 166
Msa0646710 AT4G36540.2 53.254 169 58 4 89 257 93 240 1.78e-47 163
Msa0646710 AT4G36540.1 56.129 155 47 4 89 243 93 226 1.45e-46 160
Msa0646710 AT1G10120.2 46.544 217 78 5 123 339 174 352 4.80e-46 161
Msa0646710 AT1G10120.1 46.544 217 78 5 123 339 174 352 4.80e-46 161
Msa0646710 AT1G59640.1 63.636 132 41 2 124 249 97 227 6.98e-45 155
Msa0646710 AT2G42300.4 71.134 97 28 0 148 244 175 271 1.10e-43 152
Msa0646710 AT1G59640.2 65.354 127 37 2 124 244 97 222 2.98e-43 153
Msa0646710 AT3G57800.2 63.415 123 40 2 149 271 195 312 3.60e-43 154
Msa0646710 AT5G50915.3 54.777 157 53 6 109 259 85 229 6.25e-43 150
Msa0646710 AT5G50915.4 54.777 157 53 6 109 259 85 229 6.25e-43 150
Msa0646710 AT5G50915.1 54.777 157 53 6 109 259 85 229 2.42e-42 149
Msa0646710 AT5G50915.2 54.777 157 53 6 109 259 85 229 2.42e-42 149
Msa0646710 AT2G42300.1 70.833 96 28 0 148 243 175 270 3.61e-42 150
Msa0646710 AT5G62610.2 77.647 85 15 1 149 229 140 224 1.56e-37 136
Msa0646710 AT5G62610.1 77.647 85 15 1 149 229 140 224 1.56e-37 136
Msa0646710 AT1G18400.2 76.250 80 19 0 156 235 143 222 2.39e-35 129
Msa0646710 AT1G18400.1 76.250 80 19 0 156 235 143 222 4.19e-35 129
Msa0646710 AT1G73830.2 77.215 79 18 0 157 235 146 224 6.71e-35 129
Msa0646710 AT1G73830.1 77.215 79 18 0 157 235 146 224 7.55e-35 129
Msa0646710 AT3G57800.1 45.882 170 40 3 149 271 195 359 2.45e-34 131
Msa0646710 AT1G25330.1 64.286 98 34 1 149 245 94 191 3.32e-34 126
Msa0646710 AT5G58010.1 60.000 90 28 3 148 233 92 177 6.45e-23 97.4
Msa0646710 AT2G24260.2 56.471 85 36 1 139 223 16 99 3.27e-22 94.7
Msa0646710 AT4G30980.1 71.212 66 18 1 158 223 130 194 8.67e-22 94.7
Msa0646710 AT4G30980.2 71.212 66 18 1 158 223 178 242 1.42e-21 94.7
Msa0646710 AT2G24260.1 56.471 85 36 1 139 223 119 202 2.25e-21 94.4
Msa0646710 AT2G42300.5 70.000 50 15 0 148 197 175 224 3.53e-18 82.8
Msa0646710 AT2G42300.3 70.000 50 15 0 148 197 175 224 3.53e-18 82.8
Msa0646710 AT2G42300.2 70.000 50 15 0 148 197 175 224 4.52e-18 82.8
Msa0646710 AT2G20180.7 53.968 63 28 1 161 223 210 271 2.54e-14 73.9
Msa0646710 AT2G20180.1 53.968 63 28 1 161 223 210 271 2.54e-14 73.9
Msa0646710 AT2G20180.5 53.968 63 28 1 161 223 210 271 2.54e-14 73.9
Msa0646710 AT2G20180.4 53.968 63 28 1 161 223 281 342 2.75e-14 74.3
Msa0646710 AT2G20180.6 53.968 63 28 1 161 223 281 342 2.75e-14 74.3
Msa0646710 AT2G20180.2 53.968 63 28 1 161 223 281 342 2.75e-14 74.3
Msa0646710 AT2G20180.3 53.968 63 28 1 161 223 281 342 2.75e-14 74.3
Msa0646710 AT1G09530.3 46.479 71 37 1 153 223 332 401 4.47e-14 73.6
Msa0646710 AT1G09530.2 46.479 71 37 1 153 223 332 401 4.47e-14 73.6
Msa0646710 AT1G09530.5 46.479 71 37 1 153 223 332 401 4.47e-14 73.6
Msa0646710 AT1G09530.1 46.479 71 37 1 153 223 332 401 4.47e-14 73.6
Msa0646710 AT1G09530.4 46.479 71 37 1 153 223 332 401 4.47e-14 73.6
Msa0646710 AT1G09530.6 46.479 71 37 1 153 223 332 401 4.47e-14 73.6
Msa0646710 AT4G09180.1 50.746 67 33 0 156 222 182 248 6.87e-14 71.2
Msa0646710 AT2G42280.3 37.705 122 70 3 102 221 227 344 8.74e-14 72.0
Msa0646710 AT2G42280.1 36.667 120 72 2 102 221 227 342 8.78e-14 72.0
Msa0646710 AT4G28790.1 35.043 117 67 3 160 275 273 381 1.01e-13 72.4
Msa0646710 AT1G51140.1 54.688 64 29 0 158 221 304 367 1.19e-13 72.0
Msa0646710 AT4G02590.3 51.724 87 33 2 145 223 62 147 1.69e-13 70.1
Msa0646710 AT4G28800.1 32.667 150 77 5 119 267 224 350 2.06e-13 71.6
Msa0646710 AT4G02590.2 51.724 87 33 2 145 223 125 210 2.70e-13 70.1
Msa0646710 AT4G02590.1 51.724 87 33 2 145 223 125 210 2.70e-13 70.1
Msa0646710 AT1G03040.2 62.121 66 24 1 158 223 139 203 3.37e-13 69.7
Msa0646710 AT1G35460.1 50.769 65 32 0 158 222 181 245 3.40e-13 69.3
Msa0646710 AT1G03040.1 62.121 66 24 1 158 223 144 208 3.66e-13 69.7
Msa0646710 AT4G28815.2 55.172 58 25 1 161 218 71 127 4.35e-13 68.6
Msa0646710 AT2G20180.8 52.459 61 28 1 160 220 280 339 5.22e-13 69.7
Msa0646710 AT1G03040.3 62.121 66 24 1 158 223 144 208 6.51e-13 69.3
Msa0646710 AT4G28815.1 48.571 70 34 2 161 229 147 215 1.16e-12 68.2
Msa0646710 AT1G27740.1 38.938 113 68 1 110 222 119 230 2.23e-12 67.0
Msa0646710 AT4G28800.2 39.604 101 46 3 119 219 236 321 1.04e-11 66.2
Msa0646710 AT2G46970.1 41.250 80 41 2 156 230 216 294 1.28e-11 65.9
Msa0646710 AT2G46970.2 41.250 80 41 2 156 230 185 263 1.36e-11 65.9
Msa0646710 AT2G46970.3 41.250 80 41 2 156 230 194 272 1.43e-11 65.9
Msa0646710 AT2G14760.1 39.655 116 56 4 117 222 189 300 1.49e-11 65.1
Msa0646710 AT4G33880.1 43.011 93 49 3 133 222 238 329 4.56e-11 63.9
Msa0646710 AT4G00050.2 49.231 65 32 1 158 222 105 168 7.95e-11 62.8

Find 78 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTTTGCTCAGTGATTT+TGG 0.069577 4_3:+52554851 None:intergenic
TTAAAGTACATAACATATTT+TGG 0.158845 4_3:-52552867 Msa0646710:three_prime_UTR
CATCTGATCCATTGCAAATT+AGG 0.160930 4_3:-52552715 Msa0646710:three_prime_UTR
CACTTTGATCCTCTTGTCTT+TGG 0.165749 4_3:+52554893 None:intergenic
GGGATAGTCCCTCCGGAAAT+TGG 0.186320 4_3:-52553562 Msa0646710:CDS
TTACATTGTCTTAACACTTC+TGG 0.231856 4_3:-52555188 Msa0646710:CDS
CTGTTACATTCTCCTTCTTT+TGG 0.239090 4_3:-52552806 Msa0646710:three_prime_UTR
GACATTTCTGATTGCATCAT+TGG 0.242720 4_3:+52553658 None:intergenic
TTCCTTCGAACCCCAATTTC+CGG 0.243538 4_3:+52553550 None:intergenic
AATTTGTATCTACCTGTAAA+TGG 0.288243 4_3:+52553271 None:intergenic
TTTCCTTCTCAACCATTTAC+AGG 0.299320 4_3:-52553283 Msa0646710:intron
GATGAGTCAAGAAAGATTTC+AGG 0.310824 4_3:+52553499 None:intergenic
TGCAGGTTTAATGTCCGTTT+TGG 0.317732 4_3:+52552646 None:intergenic
GTTGGTTGTGATTCTGCTCT+TGG 0.325858 4_3:-52555014 Msa0646710:CDS
GCATTGTGTCCATACCTCTT+TGG 0.342272 4_3:+52553934 None:intergenic
TGGTGGATTGATAAACAATA+TGG 0.350380 4_3:-52553584 Msa0646710:CDS
GGATAGTCCCTCCGGAAATT+GGG 0.350458 4_3:-52553561 Msa0646710:CDS
AACAAAATTGCAGGCAAAGC+TGG 0.365496 4_3:-52554215 Msa0646710:CDS
CATTAAACCTGCAAGAAATT+CGG 0.370651 4_3:-52552636 Msa0646710:three_prime_UTR
CAACACTGTCATGTCGGAAA+TGG 0.383436 4_3:+52555148 None:intergenic
TCATGAACCGAATTTCTTGC+AGG 0.397044 4_3:+52552629 None:intergenic
ATCTGATCCATTGCAAATTA+GGG 0.401608 4_3:-52552714 Msa0646710:three_prime_UTR
CAATGTTGAAGTCAAGTCTT+GGG 0.403046 4_3:+52553969 None:intergenic
CAAAAGAAGGAGAATGTAAC+AGG 0.403302 4_3:+52552807 None:intergenic
CCAGGATGTAACAAAATTGC+AGG 0.430070 4_3:-52554224 Msa0646710:CDS
TGATGAATTGTTTGCCAAAG+AGG 0.438799 4_3:-52553948 Msa0646710:intron
GCTCGAACATGAATGTAATC+AGG 0.466193 4_3:+52554735 None:intergenic
TCAATGTTGAAGTCAAGTCT+TGG 0.479932 4_3:+52553968 None:intergenic
GAATTGAATTGAAGATATGC+AGG 0.482069 4_3:+52553631 None:intergenic
AAAGAGAGAGATTTGTGAGC+AGG 0.484444 4_3:-52552738 Msa0646710:three_prime_UTR
ATCAAAGTGAGTGTTGAAGA+AGG 0.498660 4_3:-52554879 Msa0646710:CDS
TCGAAGAAACCATTAAGAAA+AGG 0.502237 4_3:-52554944 Msa0646710:CDS
AACACTTCTGGAAATCTAGT+AGG 0.504501 4_3:-52555176 Msa0646710:CDS
TAACTTGAATGTCCAAAAGA+AGG 0.504694 4_3:+52552794 None:intergenic
ATATCCTACCATCCTCAACT+TGG 0.507057 4_3:-52553363 Msa0646710:CDS
AAAATCACCTTCCCAAGTTG+AGG 0.508180 4_3:+52553351 None:intergenic
CAATATGGGGATAGTCCCTC+CGG 0.510922 4_3:-52553569 Msa0646710:CDS
TATTTGCAAGATTTAGTACC+AGG 0.510967 4_3:-52554242 Msa0646710:CDS
GGTGGATTGATAAACAATAT+GGG 0.517497 4_3:-52553583 Msa0646710:CDS
TAACTCTATGAAACCCGACC+CGG 0.524469 4_3:-52554979 Msa0646710:CDS
TGAAGCTAGCAATCTGAAGA+TGG 0.529573 4_3:-52552949 Msa0646710:CDS
GTTCGTGTTCATGTTCATGT+TGG 0.539052 4_3:+52555099 None:intergenic
GTCTCTTCAAAGACAAGTTG+AGG 0.543289 4_3:-52554162 Msa0646710:intron
CAACAACTAGTTTCATGTTG+TGG 0.545151 4_3:-52553604 Msa0646710:CDS
AGAGAAAATTGTGATGATGT+TGG 0.545309 4_3:-52554801 Msa0646710:CDS
AAACATTGAATCAAATGCAA+TGG 0.546511 4_3:+52552675 None:intergenic
ATTGAATCAAATGCAATGGA+AGG 0.547369 4_3:+52552679 None:intergenic
CTCCGGAAATTGGGGTTCGA+AGG 0.548238 4_3:-52553552 Msa0646710:CDS
CTACCTGTAAATGGTTGAGA+AGG 0.549856 4_3:+52553280 None:intergenic
CATTTCCGACATGACAGTGT+TGG 0.552355 4_3:-52555147 Msa0646710:CDS
CTTCCCAAGTTGAGGATGGT+AGG 0.556292 4_3:+52553359 None:intergenic
CAACTAGTTTCATGTTGTGG+TGG 0.563540 4_3:-52553601 Msa0646710:CDS
AAAATCACTGAGCAAACAAA+GGG 0.564488 4_3:-52554849 Msa0646710:CDS
GATAGTCCCTCCGGAAATTG+GGG 0.565816 4_3:-52553560 Msa0646710:CDS
ATTGCAAATTAGGGTGTTGG+TGG 0.566623 4_3:-52552705 Msa0646710:three_prime_UTR
TTGTGATGATGTTGGTTCCA+AGG 0.568124 4_3:-52554793 Msa0646710:CDS
TCCATTGCAAATTAGGGTGT+TGG 0.573419 4_3:-52552708 Msa0646710:three_prime_UTR
TCAGGTAAAGATGCAGATGC+CGG 0.578456 4_3:+52553517 None:intergenic
TCGAAGGAACATTAATGCAC+CGG 0.582886 4_3:-52553536 Msa0646710:CDS
CAAAATCACTGAGCAAACAA+AGG 0.583100 4_3:-52554850 Msa0646710:CDS
CTACCATCCTCAACTTGGGA+AGG 0.586675 4_3:-52553358 Msa0646710:CDS
ACCAACACCCTAATTTGCAA+TGG 0.594011 4_3:+52552707 None:intergenic
GGTTCCAAGGAAAATTCGAA+AGG 0.600922 4_3:-52554780 Msa0646710:CDS
TATCCTACCATCCTCAACTT+GGG 0.602359 4_3:-52553362 Msa0646710:CDS
AATTGAATTGAAGATATGCA+GGG 0.606589 4_3:+52553632 None:intergenic
TCTTTCCAACACTGTCATGT+CGG 0.609762 4_3:+52555142 None:intergenic
ACTTCTGGAAATCTAGTAGG+AGG 0.609823 4_3:-52555173 Msa0646710:CDS
TCACCTTCCCAAGTTGAGGA+TGG 0.614865 4_3:+52553355 None:intergenic
CTTCGAACCCCAATTTCCGG+AGG 0.622595 4_3:+52553553 None:intergenic
TTCGAACCCCAATTTCCGGA+GGG 0.626102 4_3:+52553554 None:intergenic
TGTTGGAAAGACAAAGAGCA+AGG 0.628279 4_3:-52555130 Msa0646710:CDS
AACTCTATGAAACCCGACCC+GGG 0.629719 4_3:-52554978 Msa0646710:CDS
GAAAAGGAAAGCTGATAACA+AGG 0.638853 4_3:-52554928 Msa0646710:CDS
ATATAAAGTCCAAAGACAAG+AGG 0.657536 4_3:-52554902 Msa0646710:CDS
ATTCATGTTCGAGCACGTCG+CGG 0.664373 4_3:-52554726 Msa0646710:CDS
GTGGATTGATAAACAATATG+GGG 0.684555 4_3:-52553582 Msa0646710:CDS
AAGGAAAGCTGATAACAAGG+TGG 0.715030 4_3:-52554925 Msa0646710:CDS
CATTTCCCGAAAAGTAACGT+CGG 0.737392 4_3:+52555063 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTACTAAATTGTTTAATT+TGG - chr4_3:52553351-52553370 Msa0646710:CDS 10.0%
!!! TTAAGTTTAAGTTTATAATA+TGG - chr4_3:52553733-52553752 Msa0646710:intron 10.0%
!!! ATTTTATGGTTTTAGTAAAA+AGG - chr4_3:52553908-52553927 Msa0646710:intron 15.0%
!! TTAAATTATTTGATCTTTAC+AGG - chr4_3:52554118-52554137 Msa0646710:intron 15.0%
!! AATCATATTAATGATTGATT+AGG + chr4_3:52554394-52554413 None:intergenic 15.0%
!!! TTAAAGTACATAACATATTT+TGG - chr4_3:52554949-52554968 Msa0646710:CDS 15.0%
!!! TCTCTTAATTTGTCATTATT+TGG - chr4_3:52553181-52553200 Msa0646710:intron 20.0%
!! TAGTAAAAAATGATGTAACA+CGG - chr4_3:52553252-52553271 Msa0646710:intron 20.0%
!! ATGCTAAAATTACAGTTAAT+AGG - chr4_3:52553378-52553397 Msa0646710:intron 20.0%
!!! TGATTTTTCTGTTTATTACT+CGG - chr4_3:52553483-52553502 Msa0646710:intron 20.0%
!! GTATGATAAATTAGTTACTT+AGG - chr4_3:52553688-52553707 Msa0646710:intron 20.0%
!!! AAATCTTCATTTTGATGAAA+AGG + chr4_3:52554696-52554715 None:intergenic 20.0%
!! ATGATTGAAAGTTGAAAATT+CGG - chr4_3:52554747-52554766 Msa0646710:CDS 20.0%
!!! TTTCTTATTCTTTTTTCTAG+TGG + chr4_3:52555056-52555075 None:intergenic 20.0%
!! ATCTTCACAACAATTTTTGA+TGG - chr4_3:52552780-52552799 Msa0646710:three_prime_UTR 25.0%
! CTAAAATTACAGTTAATAGG+AGG - chr4_3:52553381-52553400 Msa0646710:intron 25.0%
! ACACTTATTATGTGCAATAT+AGG - chr4_3:52553449-52553468 Msa0646710:intron 25.0%
! AATTGAATTGAAGATATGCA+GGG + chr4_3:52554187-52554206 None:intergenic 25.0%
!!! GATTAGGAACAATATTTTAG+AGG + chr4_3:52554378-52554397 None:intergenic 25.0%
!! TTTTCAAAATCTTCATAGTG+TGG - chr4_3:52554484-52554503 Msa0646710:intron 25.0%
! AATTTGTATCTACCTGTAAA+TGG + chr4_3:52554548-52554567 None:intergenic 25.0%
!!! GAATTTTGATAAGAAGCATT+TGG + chr4_3:52554921-52554940 None:intergenic 25.0%
! AAACATTGAATCAAATGCAA+TGG + chr4_3:52555144-52555163 None:intergenic 25.0%
!!! CAATGTTTTGAAAACCAAAA+CGG - chr4_3:52555156-52555175 Msa0646710:CDS 25.0%
TTACATTGTCTTAACACTTC+TGG - chr4_3:52552628-52552647 Msa0646710:three_prime_UTR 30.0%
!! TCACAACAATTTTTGATGGT+TGG - chr4_3:52552784-52552803 Msa0646710:three_prime_UTR 30.0%
TCGAAGAAACCATTAAGAAA+AGG - chr4_3:52552872-52552891 Msa0646710:three_prime_UTR 30.0%
! ATCAGCTTTCCTTTTCTTAA+TGG + chr4_3:52552884-52552903 None:intergenic 30.0%
ATATAAAGTCCAAAGACAAG+AGG - chr4_3:52552914-52552933 Msa0646710:three_prime_UTR 30.0%
AAAATCACTGAGCAAACAAA+GGG - chr4_3:52552967-52552986 Msa0646710:intron 30.0%
! AGAGAAAATTGTGATGATGT+TGG - chr4_3:52553015-52553034 Msa0646710:intron 30.0%
GTTTATTACTCGGAGTTTAA+AGG - chr4_3:52553493-52553512 Msa0646710:CDS 30.0%
TATTTGCAAGATTTAGTACC+AGG - chr4_3:52553574-52553593 Msa0646710:CDS 30.0%
! GGACACAATGCAAAATTTTA+TGG - chr4_3:52553894-52553913 Msa0646710:intron 30.0%
TCTACATTTGACCGTAATTA+AGG - chr4_3:52553991-52554010 Msa0646710:CDS 30.0%
! CAAAAAGCGCAAATCATTAA+TGG + chr4_3:52554073-52554092 None:intergenic 30.0%
GAATTGAATTGAAGATATGC+AGG + chr4_3:52554188-52554207 None:intergenic 30.0%
!! TGGTGGATTGATAAACAATA+TGG - chr4_3:52554232-52554251 Msa0646710:CDS 30.0%
!! GGTGGATTGATAAACAATAT+GGG - chr4_3:52554233-52554252 Msa0646710:CDS 30.0%
! GTGGATTGATAAACAATATG+GGG - chr4_3:52554234-52554253 Msa0646710:CDS 30.0%
!! AGGAACAATATTTTAGAGGA+CGG + chr4_3:52554374-52554393 None:intergenic 30.0%
AAGGACTTATCAATATCTGT+GGG + chr4_3:52554677-52554696 None:intergenic 30.0%
AAAGGACTTATCAATATCTG+TGG + chr4_3:52554678-52554697 None:intergenic 30.0%
!!! ATGTTTTGTTTTATGACTGC+AGG - chr4_3:52554838-52554857 Msa0646710:CDS 30.0%
TAACTTGAATGTCCAAAAGA+AGG + chr4_3:52555025-52555044 None:intergenic 30.0%
ATCTGATCCATTGCAAATTA+GGG - chr4_3:52555102-52555121 Msa0646710:CDS 30.0%
ATTGAATCAAATGCAATGGA+AGG + chr4_3:52555140-52555159 None:intergenic 30.0%
CATTAAACCTGCAAGAAATT+CGG - chr4_3:52555180-52555199 Msa0646710:CDS 30.0%
! AACACTTCTGGAAATCTAGT+AGG - chr4_3:52552640-52552659 Msa0646710:three_prime_UTR 35.0%
GAAAAGGAAAGCTGATAACA+AGG - chr4_3:52552888-52552907 Msa0646710:three_prime_UTR 35.0%
!! ATCAAAGTGAGTGTTGAAGA+AGG - chr4_3:52552937-52552956 Msa0646710:exon 35.0%
!! CTTTGTTTGCTCAGTGATTT+TGG + chr4_3:52552968-52552987 None:intergenic 35.0%
CAAAATCACTGAGCAAACAA+AGG - chr4_3:52552966-52552985 Msa0646710:intron 35.0%
! TGAACCTTTCGAATTTTCCT+TGG + chr4_3:52553043-52553062 None:intergenic 35.0%
TATCCATCAATCATGATCGT+CGG + chr4_3:52553291-52553310 None:intergenic 35.0%
! ATGTGCAATATAGGTTAGCT+CGG - chr4_3:52553458-52553477 Msa0646710:intron 35.0%
GAAGGGAAAAAATAAGCGAA+AGG - chr4_3:52553545-52553564 Msa0646710:CDS 35.0%
TCATTGACAAGAACTGCATT+TGG + chr4_3:52553811-52553830 None:intergenic 35.0%
CAATGTTGAAGTCAAGTCTT+GGG + chr4_3:52553850-52553869 None:intergenic 35.0%
TCAATGTTGAAGTCAAGTCT+TGG + chr4_3:52553851-52553870 None:intergenic 35.0%
TGATGAATTGTTTGCCAAAG+AGG - chr4_3:52553868-52553887 Msa0646710:intron 35.0%
AATTGTTTGCCAAAGAGGTA+TGG - chr4_3:52553873-52553892 Msa0646710:intron 35.0%
AGATGATACAGCCTTAATCA+CGG + chr4_3:52553974-52553993 None:intergenic 35.0%
GACTCATAACGCCTTAATTA+CGG + chr4_3:52554005-52554024 None:intergenic 35.0%
GACATTTCTGATTGCATCAT+TGG + chr4_3:52554161-52554180 None:intergenic 35.0%
! CAACAACTAGTTTCATGTTG+TGG - chr4_3:52554212-52554231 Msa0646710:CDS 35.0%
GATGAGTCAAGAAAGATTTC+AGG + chr4_3:52554320-52554339 None:intergenic 35.0%
!! CATAGTGTGGATTTTGATCA+AGG - chr4_3:52554497-52554516 Msa0646710:intron 35.0%
TTTCCTTCTCAACCATTTAC+AGG - chr4_3:52554533-52554552 Msa0646710:intron 35.0%
!! CTTTCTACTTCTAAGATTGC+AGG + chr4_3:52554601-52554620 None:intergenic 35.0%
TCTCAAGTCTCTATCATCTA+TGG + chr4_3:52554652-52554671 None:intergenic 35.0%
AGGACTTATCAATATCTGTG+GGG + chr4_3:52554676-52554695 None:intergenic 35.0%
CCTTGCAAACCTTTATATTC+CGG - chr4_3:52554779-52554798 Msa0646710:CDS 35.0%
CTTTATATTCCGGAAAAACG+CGG - chr4_3:52554789-52554808 Msa0646710:CDS 35.0%
CAAAAGAAGGAGAATGTAAC+AGG + chr4_3:52555012-52555031 None:intergenic 35.0%
! CTGTTACATTCTCCTTCTTT+TGG - chr4_3:52555010-52555029 Msa0646710:CDS 35.0%
CATCTGATCCATTGCAAATT+AGG - chr4_3:52555101-52555120 Msa0646710:CDS 35.0%
! ACTTCTGGAAATCTAGTAGG+AGG - chr4_3:52552643-52552662 Msa0646710:three_prime_UTR 40.0%
TCTTTCCAACACTGTCATGT+CGG + chr4_3:52552677-52552696 None:intergenic 40.0%
TGTTGGAAAGACAAAGAGCA+AGG - chr4_3:52552686-52552705 Msa0646710:three_prime_UTR 40.0%
GTTCGTGTTCATGTTCATGT+TGG + chr4_3:52552720-52552739 None:intergenic 40.0%
CATTTCCCGAAAAGTAACGT+CGG + chr4_3:52552756-52552775 None:intergenic 40.0%
AAGGAAAGCTGATAACAAGG+TGG - chr4_3:52552891-52552910 Msa0646710:three_prime_UTR 40.0%
CACTTTGATCCTCTTGTCTT+TGG + chr4_3:52552926-52552945 None:intergenic 40.0%
!! TTGTGATGATGTTGGTTCCA+AGG - chr4_3:52553023-52553042 Msa0646710:intron 40.0%
GGTTCCAAGGAAAATTCGAA+AGG - chr4_3:52553036-52553055 Msa0646710:intron 40.0%
GCTCGAACATGAATGTAATC+AGG + chr4_3:52553084-52553103 None:intergenic 40.0%
ATTCCGACGATCATGATTGA+TGG - chr4_3:52553285-52553304 Msa0646710:CDS 40.0%
GCACAAAACAGAACAAACGT+TGG + chr4_3:52553519-52553538 None:intergenic 40.0%
! ACGTTTGTTCTGTTTTGTGC+AGG - chr4_3:52553519-52553538 Msa0646710:CDS 40.0%
! TCTGTTTTGTGCAGGTTAGA+AGG - chr4_3:52553527-52553546 Msa0646710:CDS 40.0%
! CTGTTTTGTGCAGGTTAGAA+GGG - chr4_3:52553528-52553547 Msa0646710:CDS 40.0%
! CCTGCAATTTTGTTACATCC+TGG + chr4_3:52553595-52553614 None:intergenic 40.0%
CCAGGATGTAACAAAATTGC+AGG - chr4_3:52553592-52553611 Msa0646710:CDS 40.0%
AACAAAATTGCAGGCAAAGC+TGG - chr4_3:52553601-52553620 Msa0646710:CDS 40.0%
GTCTCTTCAAAGACAAGTTG+AGG - chr4_3:52553654-52553673 Msa0646710:CDS 40.0%
!! GGTTTTAGTAAAAAGGTCCG+CGG - chr4_3:52553915-52553934 Msa0646710:intron 40.0%
GGCATCAAAAATCATGATGC+TGG + chr4_3:52553941-52553960 None:intergenic 40.0%
! GCATCATGATTTTTGATGCC+AGG - chr4_3:52553941-52553960 Msa0646710:intron 40.0%
TGTTGTGATTCGCGATATTG+TGG + chr4_3:52554047-52554066 None:intergenic 40.0%
! CAACTAGTTTCATGTTGTGG+TGG - chr4_3:52554215-52554234 Msa0646710:CDS 40.0%
TCGAAGGAACATTAATGCAC+CGG - chr4_3:52554280-52554299 Msa0646710:intron 40.0%
ATATCCTACCATCCTCAACT+TGG - chr4_3:52554453-52554472 Msa0646710:intron 40.0%
TATCCTACCATCCTCAACTT+GGG - chr4_3:52554454-52554473 Msa0646710:intron 40.0%
AAAATCACCTTCCCAAGTTG+AGG + chr4_3:52554468-52554487 None:intergenic 40.0%
CTACCTGTAAATGGTTGAGA+AGG + chr4_3:52554539-52554558 None:intergenic 40.0%
!! ACTTCTAAGATTGCAGGCTA+AGG + chr4_3:52554595-52554614 None:intergenic 40.0%
AAAGGTTTGCAAGGTAACCA+CGG + chr4_3:52554773-52554792 None:intergenic 40.0%
CCGGAATATAAAGGTTTGCA+AGG + chr4_3:52554782-52554801 None:intergenic 40.0%
! CGCGTTTTTCCGGAATATAA+AGG + chr4_3:52554791-52554810 None:intergenic 40.0%
TGAAGCTAGCAATCTGAAGA+TGG - chr4_3:52554867-52554886 Msa0646710:CDS 40.0%
TTGGAGTGAGCAAAAAGTTC+AGG + chr4_3:52554902-52554921 None:intergenic 40.0%
AAAGAGAGAGATTTGTGAGC+AGG - chr4_3:52555078-52555097 Msa0646710:CDS 40.0%
TCCATTGCAAATTAGGGTGT+TGG - chr4_3:52555108-52555127 Msa0646710:CDS 40.0%
! ACCAACACCCTAATTTGCAA+TGG + chr4_3:52555112-52555131 None:intergenic 40.0%
!! ATTGCAAATTAGGGTGTTGG+TGG - chr4_3:52555111-52555130 Msa0646710:CDS 40.0%
! TGCAGGTTTAATGTCCGTTT+TGG + chr4_3:52555173-52555192 None:intergenic 40.0%
TCATGAACCGAATTTCTTGC+AGG + chr4_3:52555190-52555209 None:intergenic 40.0%
CAACACTGTCATGTCGGAAA+TGG + chr4_3:52552671-52552690 None:intergenic 45.0%
CATTTCCGACATGACAGTGT+TGG - chr4_3:52552669-52552688 Msa0646710:three_prime_UTR 45.0%
!!! GCAACACCGACGTTACTTTT+CGG - chr4_3:52552747-52552766 Msa0646710:three_prime_UTR 45.0%
!!! CAACACCGACGTTACTTTTC+GGG - chr4_3:52552748-52552767 Msa0646710:three_prime_UTR 45.0%
!!! CGACGTTACTTTTCGGGAAA+TGG - chr4_3:52552754-52552773 Msa0646710:three_prime_UTR 45.0%
GTTGGTTGTGATTCTGCTCT+TGG - chr4_3:52552802-52552821 Msa0646710:three_prime_UTR 45.0%
TAACTCTATGAAACCCGACC+CGG - chr4_3:52552837-52552856 Msa0646710:three_prime_UTR 45.0%
! TCTTCGACGTTTTCGAAACC+CGG + chr4_3:52552859-52552878 None:intergenic 45.0%
GCATTGTGTCCATACCTCTT+TGG + chr4_3:52553885-52553904 None:intergenic 45.0%
AAAAATCATGATGCTGGCCG+CGG + chr4_3:52553935-52553954 None:intergenic 45.0%
CGCGATATTGTGGATAATCG+CGG + chr4_3:52554037-52554056 None:intergenic 45.0%
TTCCTTCGAACCCCAATTTC+CGG + chr4_3:52554269-52554288 None:intergenic 45.0%
TCAGGTAAAGATGCAGATGC+CGG + chr4_3:52554302-52554321 None:intergenic 45.0%
GAAAGTTGAAAATTCGGCCG+TGG - chr4_3:52554753-52554772 Msa0646710:CDS 45.0%
! TCGAATCTACCGCGTTTTTC+CGG + chr4_3:52554801-52554820 None:intergenic 45.0%
AAAACGCGGTAGATTCGATG+CGG - chr4_3:52554803-52554822 Msa0646710:CDS 45.0%
AACTCTATGAAACCCGACCC+GGG - chr4_3:52552838-52552857 Msa0646710:three_prime_UTR 50.0%
! CTTCGACGTTTTCGAAACCC+GGG + chr4_3:52552858-52552877 None:intergenic 50.0%
ATTCATGTTCGAGCACGTCG+CGG - chr4_3:52553090-52553109 Msa0646710:intron 50.0%
TACAGCCTTAATCACGGTCG+TGG + chr4_3:52553968-52553987 None:intergenic 50.0%
CAATATGGGGATAGTCCCTC+CGG - chr4_3:52554247-52554266 Msa0646710:CDS 50.0%
GGATAGTCCCTCCGGAAATT+GGG - chr4_3:52554255-52554274 Msa0646710:CDS 50.0%
GATAGTCCCTCCGGAAATTG+GGG - chr4_3:52554256-52554275 Msa0646710:CDS 50.0%
TTCGAACCCCAATTTCCGGA+GGG + chr4_3:52554265-52554284 None:intergenic 50.0%
TAGAGGACGGATACACAGAG+AGG + chr4_3:52554361-52554380 None:intergenic 50.0%
! CTTCCCAAGTTGAGGATGGT+AGG + chr4_3:52554460-52554479 None:intergenic 50.0%
CTACCATCCTCAACTTGGGA+AGG - chr4_3:52554458-52554477 Msa0646710:intron 50.0%
! TCACCTTCCCAAGTTGAGGA+TGG + chr4_3:52554464-52554483 None:intergenic 50.0%
AAACGCGGTAGATTCGATGC+GGG - chr4_3:52554804-52554823 Msa0646710:CDS 50.0%
! ACGTTTTCGAAACCCGGGTC+GGG + chr4_3:52552853-52552872 None:intergenic 55.0%
! GACGTTTTCGAAACCCGGGT+CGG + chr4_3:52552854-52552873 None:intergenic 55.0%
CTTAATCACGGTCGTGGACC+TGG + chr4_3:52553962-52553981 None:intergenic 55.0%
CAGGTCCACGACCGTGATTA+AGG - chr4_3:52553960-52553979 Msa0646710:CDS 55.0%
GGGATAGTCCCTCCGGAAAT+TGG - chr4_3:52554254-52554273 Msa0646710:CDS 55.0%
CTTCGAACCCCAATTTCCGG+AGG + chr4_3:52554266-52554285 None:intergenic 55.0%
CTCCGGAAATTGGGGTTCGA+AGG - chr4_3:52554264-52554283 Msa0646710:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 52552624 52555214 52552624 ID=Msa0646710;Name=Msa0646710
chr4_3 mRNA 52552624 52555214 52552624 ID=Msa0646710-mRNA-1;Parent=Msa0646710;Name=Msa0646710-mRNA-1;_AED=0.12;_eAED=0.12;_QI=1|1|1|1|1|1|6|317|359
chr4_3 exon 52552624 52552978 52552624 ID=Msa0646710-mRNA-1:exon:12909;Parent=Msa0646710-mRNA-1
chr4_3 exon 52553284 52553386 52553284 ID=Msa0646710-mRNA-1:exon:12908;Parent=Msa0646710-mRNA-1
chr4_3 exon 52553489 52553698 52553489 ID=Msa0646710-mRNA-1:exon:12907;Parent=Msa0646710-mRNA-1
chr4_3 exon 52553949 52554020 52553949 ID=Msa0646710-mRNA-1:exon:12906;Parent=Msa0646710-mRNA-1
chr4_3 exon 52554163 52554297 52554163 ID=Msa0646710-mRNA-1:exon:12905;Parent=Msa0646710-mRNA-1
chr4_3 exon 52554692 52555214 52554692 ID=Msa0646710-mRNA-1:exon:12904;Parent=Msa0646710-mRNA-1
chr4_3 five_prime_UTR 52555214 52555214 52555214 ID=Msa0646710-mRNA-1:five_prime_utr;Parent=Msa0646710-mRNA-1
chr4_3 CDS 52554692 52555213 52554692 ID=Msa0646710-mRNA-1:cds;Parent=Msa0646710-mRNA-1
chr4_3 CDS 52554163 52554297 52554163 ID=Msa0646710-mRNA-1:cds;Parent=Msa0646710-mRNA-1
chr4_3 CDS 52553949 52554020 52553949 ID=Msa0646710-mRNA-1:cds;Parent=Msa0646710-mRNA-1
chr4_3 CDS 52553489 52553698 52553489 ID=Msa0646710-mRNA-1:cds;Parent=Msa0646710-mRNA-1
chr4_3 CDS 52553284 52553386 52553284 ID=Msa0646710-mRNA-1:cds;Parent=Msa0646710-mRNA-1
chr4_3 CDS 52552941 52552978 52552941 ID=Msa0646710-mRNA-1:cds;Parent=Msa0646710-mRNA-1
chr4_3 three_prime_UTR 52552624 52552940 52552624 ID=Msa0646710-mRNA-1:three_prime_utr;Parent=Msa0646710-mRNA-1
Gene Sequence

>Msa0646710

ATGTTACATTGTCTTAACACTTCTGGAAATCTAGTAGGAGGAACCATTTCCGACATGACAGTGTTGGAAAGACAAAGAGCAAGGAAGAAGTTCCAACATGAACATGAACACGAACAAGATCAGCAACACCGACGTTACTTTTCGGGAAATGGTTTATCTTCACAACAATTTTTGATGGTTGGTTGTGATTCTGCTCTTGGTGAAGTTGTAGCTAACTCTATGAAACCCGACCCGGGTTTCGAAAACGTCGAAGAAACCATTAAGAAAAGGAAAGCTGATAACAAGGTGGATATAAAGTCCAAAGACAAGAGGATCAAAGTGAGTGTTGAAGAAGGTGAATCCAAAATCACTGAGCAAACAAAGGGACAGAAGAATACAAAGTTGAAAAATAGAGAAAATTGTGATGATGTTGGTTCCAAGGAAAATTCGAAAGGTTCAGAGATTCAAAATCAAAAGCCTGATTACATTCATGTTCGAGCACGTCGCGGTCAAGCTACTGATAGTCATAGCTTAGCTGAAAGAGTTAGAAGGGAAAAAATAAGCGAAAGGATGAAGTATTTGCAAGATTTAGTACCAGGATGTAACAAAATTGCAGGCAAAGCTGGAATGCTTGATGAAATTATTAACTATGTTCAGTCTCTTCAAAGACAAGTTGAGTTCTTGTCAATGAAATTAGCAGCTGTAAACCCAAGACTTGACTTCAACATTGATGAATTGTTTGCCAAAGAGGTGTTTACTCAAAATTTTCCAATGATGCAATCAGAAATGTCTAACCCTGCATATCTTCAATTCAATTCAACACAACAACTAGTTTCATGTTGTGGTGGATTGATAAACAATATGGGGATAGTCCCTCCGGAAATTGGGGTTCGAAGGAACATTAATGCACCGGCATCTGCATCTTTACCTGAAATCTTTCTTGACTCATCATGTTTCACTCATATCCTACCATCCTCAACTTGGGAAGGTGATTTTCAAAATCTTCATAGTGTGGATTTTGATCAAGGAAGATCAACATCATTTCCTTCTCAACCATTTACAGGCATGATTGAAGCTAGCAATCTGAAGATGGAGATGTAA
Protein sequence

>Msa0646710

MLHCLNTSGNLVGGTISDMTVLERQRARKKFQHEHEHEQDQQHRRYFSGNGLSSQQFLMVGCDSALGEVVANSMKPDPGFENVEETIKKRKADNKVDIKSKDKRIKVSVEEGESKITEQTKGQKNTKLKNRENCDDVGSKENSKGSEIQNQKPDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKIAGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNIDELFAKEVFTQNFPMMQSEMSNPAYLQFNSTQQLVSCCGGLINNMGIVPPEIGVRRNINAPASASLPEIFLDSSCFTHILPSSTWEGDFQNLHSVDFDQGRSTSFPSQPFTGMIEASNLKMEM*