Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344940 | AES91085.2 | 88.613 | 483 | 12 | 1 | 8 | 447 | 1 | 483 | 0.0 | 865 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344940 | sp|Q93Y08|AB1K8_ARATH | 31.944 | 288 | 150 | 8 | 85 | 331 | 229 | 511 | 8.19e-32 | 132 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000220 | Msa1344940 | 0.901123 | 3.681936e-78 | -8.615850e-47 |
| Msa0000780 | Msa1344940 | 0.800659 | 1.298927e-48 | -8.615850e-47 |
| Msa0001420 | Msa1344940 | 0.808296 | 3.321413e-50 | -8.615850e-47 |
| Msa0001460 | Msa1344940 | 0.863079 | 3.216235e-64 | -8.615850e-47 |
| Msa0001510 | Msa1344940 | 0.854578 | 1.120956e-61 | -8.615850e-47 |
| Msa0001540 | Msa1344940 | 0.877410 | 6.426780e-69 | -8.615850e-47 |
| Msa0002060 | Msa1344940 | 0.814305 | 1.648868e-51 | -8.615850e-47 |
| Msa0006050 | Msa1344940 | 0.816830 | 4.517277e-52 | -8.615850e-47 |
| Msa0006580 | Msa1344940 | 0.825453 | 4.650007e-54 | -8.615850e-47 |
| Msa0007570 | Msa1344940 | 0.822359 | 2.471320e-53 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344940 | MtrunA17_Chr4g0057941 | 87.371 | 483 | 13 | 2 | 8 | 447 | 1 | 478 | 0.0 | 847 |
| Msa1344940 | MtrunA17_Chr1g0147481 | 27.155 | 464 | 246 | 11 | 31 | 420 | 81 | 526 | 3.43e-41 | 157 |
| Msa1344940 | MtrunA17_Chr3g0129101 | 29.714 | 350 | 190 | 10 | 27 | 331 | 158 | 496 | 5.05e-35 | 138 |
| Msa1344940 | MtrunA17_Chr8g0382921 | 27.056 | 462 | 260 | 14 | 27 | 419 | 149 | 602 | 4.39e-33 | 132 |
| Msa1344940 | MtrunA17_Chr5g0428451 | 33.173 | 208 | 134 | 3 | 85 | 288 | 135 | 341 | 6.23e-32 | 127 |
| Msa1344940 | MtrunA17_Chr6g0465161 | 27.249 | 389 | 242 | 10 | 68 | 423 | 122 | 502 | 3.10e-30 | 124 |
| Msa1344940 | MtrunA17_Chr3g0120791 | 31.365 | 271 | 163 | 9 | 27 | 283 | 148 | 409 | 7.96e-26 | 110 |
| Msa1344940 | MtrunA17_Chr4g0012061 | 24.548 | 387 | 238 | 11 | 66 | 405 | 136 | 515 | 4.95e-25 | 108 |
| Msa1344940 | MtrunA17_Chr1g0148121 | 26.780 | 295 | 172 | 8 | 75 | 330 | 131 | 420 | 4.13e-22 | 99.4 |
| Msa1344940 | MtrunA17_Chr3g0113361 | 30.317 | 221 | 144 | 6 | 71 | 285 | 83 | 299 | 6.25e-22 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1344940 | AT4G24810.2 | 66.882 | 465 | 110 | 2 | 13 | 433 | 3 | 467 | 0.0 | 641 |
| Msa1344940 | AT5G50330.1 | 66.225 | 453 | 110 | 1 | 15 | 424 | 5 | 457 | 0.0 | 631 |
| Msa1344940 | AT5G50330.2 | 64.018 | 453 | 104 | 2 | 15 | 424 | 5 | 441 | 0.0 | 601 |
| Msa1344940 | AT4G24810.6 | 66.509 | 424 | 98 | 2 | 54 | 433 | 1 | 424 | 0.0 | 580 |
| Msa1344940 | AT4G24810.1 | 66.509 | 424 | 98 | 2 | 54 | 433 | 1 | 424 | 0.0 | 580 |
| Msa1344940 | AT4G24810.3 | 65.429 | 431 | 98 | 3 | 54 | 433 | 1 | 431 | 0.0 | 573 |
| Msa1344940 | AT4G24810.4 | 65.300 | 317 | 67 | 1 | 54 | 327 | 1 | 317 | 5.15e-147 | 422 |
| Msa1344940 | AT4G24810.5 | 66.333 | 300 | 58 | 1 | 54 | 310 | 1 | 300 | 1.07e-140 | 405 |
| Msa1344940 | AT5G24810.1 | 25.786 | 477 | 283 | 13 | 31 | 442 | 55 | 525 | 1.27e-37 | 146 |
| Msa1344940 | AT5G64940.1 | 31.944 | 288 | 150 | 8 | 85 | 331 | 229 | 511 | 8.32e-33 | 132 |
Find 113 sgRNAs with CRISPR-Local
Find 619 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAGAAGGGCTCCTGTTT+TGG | 0.286377 | tig0007047:-7080 | Msa1344940:CDS |
| AAATTTCCAGAAACTAATCA+CGG | 0.603085 | tig0007047:+2021 | Msa1344940:intergenic |
| AACCAAGCCTCAATTGCTCC+GGG | 0.550223 | tig0007047:+4855 | Msa1344940:intergenic |
| AACTGAAAGGAACGTTGAAT+AGG | 0.489805 | tig0007047:+10998 | Msa1344940:intergenic |
| AAGAGAGGCATAGATCCTCA+TGG | 0.649720 | tig0007047:-6341 | Msa1344940:CDS |
| AAGATCTCCCGGAGCAATTG+AGG | 0.528522 | tig0007047:-4862 | Msa1344940:CDS |
| AAGCACAGAGGTATAATCCA+TGG | 0.586724 | tig0007047:-2228 | Msa1344940:CDS |
| AAGTTGAGTACTATCTCAAA+AGG | 0.526625 | tig0007047:-1872 | Msa1344940:three_prime_UTR |
| AATCACAAGTATAGGTGTTT+TGG | 0.342798 | tig0007047:-2049 | Msa1344940:three_prime_UTR |
| AATTATTGGGAAGCCGGATT+TGG | 0.335249 | tig0007047:-10583 | Msa1344940:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GCACATAGAGAAAATCTTAT+CGG | + | tig0007047:2350-2369 | Msa1344940:intergenic | 30.0% | |
| ATTCAGATTGCGCAAATTAT+TGG | - | tig0007047:2485-2504 | Msa1344940:intron | 30.0% | |
| TTCAGATTGCGCAAATTATT+GGG | - | tig0007047:2486-2505 | Msa1344940:intron | 30.0% | |
| TTAACAGTGTGATAAGAAGT+TGG | + | tig0007047:2575-2594 | Msa1344940:intergenic | 30.0% | |
| ATATGTTATGACACTAGTTG+AGG | - | tig0007047:2748-2767 | Msa1344940:CDS | 30.0% | |
| CTTATAGTATTTGGACCAAA+AGG | - | tig0007047:3038-3057 | Msa1344940:intron | 30.0% | |
| CGCTGCTAAAAATCAATATT+GGG | + | tig0007047:3864-3883 | Msa1344940:intergenic | 30.0% | |
| ACGCTGCTAAAAATCAATAT+TGG | + | tig0007047:3865-3884 | Msa1344940:intergenic | 30.0% | |
| CAAAAGACATATCAGTTACA+TGG | + | tig0007047:4035-4054 | Msa1344940:intergenic | 30.0% | |
| AGACAAATCTTATGAATGCA+TGG | - | tig0007047:4120-4139 | Msa1344940:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0007047 | gene | 1857 | 11247 | 1857 | ID=Msa1344940;Name=Msa1344940 |
| tig0007047 | mRNA | 1857 | 11247 | 1857 | ID=Msa1344940-mRNA-1;Parent=Msa1344940;Name=Msa1344940-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.81|0.75|1|0.81|0.83|12|340|447 |
| tig0007047 | exon | 1857 | 2261 | 1857 | ID=Msa1344940-mRNA-1:exon:4784;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 2691 | 2835 | 2691 | ID=Msa1344940-mRNA-1:exon:4783;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 3464 | 3617 | 3464 | ID=Msa1344940-mRNA-1:exon:4782;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 4794 | 4912 | 4794 | ID=Msa1344940-mRNA-1:exon:4781;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 6337 | 6427 | 6337 | ID=Msa1344940-mRNA-1:exon:4780;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 7049 | 7173 | 7049 | ID=Msa1344940-mRNA-1:exon:4779;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 8792 | 8920 | 8792 | ID=Msa1344940-mRNA-1:exon:4778;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 8996 | 9043 | 8996 | ID=Msa1344940-mRNA-1:exon:4777;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 10419 | 10613 | 10419 | ID=Msa1344940-mRNA-1:exon:4776;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 10708 | 10836 | 10708 | ID=Msa1344940-mRNA-1:exon:4775;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 10961 | 11099 | 10961 | ID=Msa1344940-mRNA-1:exon:4774;Parent=Msa1344940-mRNA-1 |
| tig0007047 | exon | 11243 | 11247 | 11243 | ID=Msa1344940-mRNA-1:exon:4773;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 11243 | 11247 | 11243 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 10961 | 11099 | 10961 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 10708 | 10836 | 10708 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 10419 | 10613 | 10419 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 8996 | 9043 | 8996 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 8792 | 8920 | 8792 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 7049 | 7173 | 7049 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 6337 | 6427 | 6337 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 4794 | 4912 | 4794 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 3464 | 3617 | 3464 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 2691 | 2835 | 2691 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | CDS | 2197 | 2261 | 2197 | ID=Msa1344940-mRNA-1:cds;Parent=Msa1344940-mRNA-1 |
| tig0007047 | three_prime_UTR | 1857 | 2196 | 1857 | ID=Msa1344940-mRNA-1:three_prime_utr;Parent=Msa1344940-mRNA-1 |
| Gene Sequence |
>Msa1344940 ATGAATGTAGTTAATTCAGTTATGTTGCCCCCTATCGTCGTTGATCTCAACGACATAAAACAGAAACTCTCCGTTCAGTTTCGTCCTATTCAACGTTCCTTTCAGTTCTGGGTTCGAGCAATTGATATTTACACCGGATACAAGGTTTTTCAATTGAGAGTTAATTTTGTGAAAGATGCACAGAAGCAAGAAGCAATGTGGGAAAGACAGCATGAGCTTGCTGCCGATAAGATTTTCTCTATGTGCTCTGACCTTGGTGGTTTCTTCCTCAAGATTGCGCAAATTATTGGGAAGCCGGATTTGGCACCTGCTGCATGGGTGAAAAGGCTGGTTACGCTCTGTGATCAAGCTCCACCAACTTCTTATCACACTGTTAAACTTGTGCTTGAGAACGAGCTTGGAATGAGTATTGATGATGTGTTTGATAGATTTGATGTTGAGCCTCTTGGTTCCGCTTCAATTGCTCAGGTTCATAAAGCAAGACTGAAAGGTGACAAAAGTGATGTTGTTGTCAAGGTTCAGCATCCTGGAGTTCAGGATCTGATGATGACAGATATACGTAACTTGCAAGCTTTTGCTTTGTACATGCAAAAAACAGATATCAAATTTGATCTATATTCGGTGACAAAGGAAATGGAAACACAGATTGGATATGAATTTGACTTCATGAGGGAGGCCAGTGCAATGGAAAAGATTAGAAAGTTCTTGTATAAAATAAACAGAAGGGCTCCTGTTTTGGTGCCAAGAGTTATACGTGATATGGTTACTAGAAAGGTCCTAGTTATGGAATATATTGACGGGATTCCAATAATGAACCTTGGTGATGAAATAGCGAAGAGAGGCATAGATCCTCATGGTAAGGTTGCCTTGCTAGATTATGGACAGGTGAAAGATCTCCCGGAGCAATTGAGGCTTGGTTATGCTAATCTCGTTCTTGCAATTGCTGACGGTGATCCAGCGAGAGCTTCAGAGAGCTATAGGGAGCTTGGTATAGATACGTTAAGCAAATGTGAAAATGAGCAACAAGAAATGCTTAAGCTGGCACAGACAATGTTTGATACAAAATTACCTCCTGGTGTAAAAATGCTGCAACCGTTTTCAGAAGAATCTTCAATTAAAAAAATTGCTGTTCAGGCTTTTCCAGAAGAACTGTTTTCTATACTTCGGACAGTGCATCTACTGAGGGGACTTAGTGTTGGACTTGGAATCAATTATTCTTGTGCGGAACAGTGGAGACCCATTGCTGAAGAAGCTCTGTCTCAAGCTGGCAGATTTAAAGGTAAACATGTTAAGCACAGAGGTATAATCCATGGTATAGTAAAAACATTATTTTGGAGAGACTAA |
| Protein sequence |
>Msa1344940 MNVVNSVMLPPIVVDLNDIKQKLSVQFRPIQRSFQFWVRAIDIYTGYKVFQLRVNFVKDAQKQEAMWERQHELAADKIFSMCSDLGGFFLKIAQIIGKPDLAPAAWVKRLVTLCDQAPPTSYHTVKLVLENELGMSIDDVFDRFDVEPLGSASIAQVHKARLKGDKSDVVVKVQHPGVQDLMMTDIRNLQAFALYMQKTDIKFDLYSVTKEMETQIGYEFDFMREASAMEKIRKFLYKINRRAPVLVPRVIRDMVTRKVLVMEYIDGIPIMNLGDEIAKRGIDPHGKVALLDYGQVKDLPEQLRLGYANLVLAIADGDPARASESYRELGIDTLSKCENEQQEMLKLAQTMFDTKLPPGVKMLQPFSEESSIKKIAVQAFPEELFSILRTVHLLRGLSVGLGINYSCAEQWRPIAEEALSQAGRFKGKHVKHRGIIHGIVKTLFWRD* |
