AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0399810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0399810 MtrunA17_Chr3g0087921 90.909 66 6 0 3 68 2 67 4.58e-35 113
Msa0399810 MtrunA17_Chr3g0087931 86.154 65 9 0 4 68 3 67 5.00e-32 105
Msa0399810 MtrunA17_Chr3g0087911 86.154 65 9 0 4 68 3 67 1.05e-24 87.0
Msa0399810 MtrunA17_Chr3g0087981 71.212 66 17 2 5 68 3 68 3.03e-23 83.6
Msa0399810 MtrunA17_Chr3g0088011 81.356 59 8 2 13 68 8 66 3.04e-23 83.2
Msa0399810 MtrunA17_Chr3g0088251 73.846 65 15 2 6 68 2 66 3.10e-23 83.2
Msa0399810 MtrunA17_Chr3g0088031 77.049 61 12 2 10 68 1 61 3.48e-23 83.2
Msa0399810 MtrunA17_Chr3g0088021 75.000 64 14 2 7 68 3 66 1.84e-22 81.3
Msa0399810 MtrunA17_Chr3g0087941 82.353 68 12 0 1 68 1 68 1.54e-21 79.0
Msa0399810 MtrunA17_Chr3g0088041 90.909 44 4 0 25 68 23 66 3.15e-21 78.2
Msa0399810 MtrunA17_Chr3g0088271 80.851 47 9 0 22 68 19 65 4.22e-20 75.5
Msa0399810 MtrunA17_Chr3g0088071 77.083 48 11 0 21 68 19 66 1.51e-19 73.9
Msa0399810 MtrunA17_Chr3g0088081 85.714 42 6 0 27 68 24 65 1.56e-19 73.9
Msa0399810 MtrunA17_Chr3g0088001 75.385 65 15 1 5 68 3 67 2.40e-19 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACAATGAAGTTGTTCTT+TGG 0.336597 3_2:+23409225 Msa0399810:CDS
CCAGCATCAGGACCTGGTGC+TGG 0.401353 3_2:-23409306 Msa0399800:intron
GCAAGTTCTGTGGCTATGAT+TGG 0.405849 3_2:+23409345 Msa0399810:CDS
CCAGCACCAGGTCCTGATGC+TGG 0.415553 3_2:+23409306 Msa0399810:CDS
GATCCGGCAGCTCCAGCATC+AGG 0.425147 3_2:-23409318 Msa0399800:intron
GGTCCTGATGCTGGAGCTGC+CGG 0.466366 3_2:+23409315 Msa0399810:CDS
CACAGAACTTGCAACAGATC+CGG 0.484083 3_2:-23409334 Msa0399800:intron
CGTCTTCGCCGCTGTAGTCT+CGG 0.497571 3_2:+23409257 Msa0399810:CDS
CTAACGACGGACTAAGGTCT+TGG 0.503269 3_2:-23409281 Msa0399800:intron
CTGGAGCTAACGACGGACTA+AGG 0.507562 3_2:-23409287 Msa0399800:intron
TAACGACGGACTAAGGTCTT+GGG 0.525930 3_2:-23409280 Msa0399800:intron
GCAGCTCCAGCATCAGGACC+TGG 0.550326 3_2:-23409312 Msa0399800:intron
CCGTCGTTAGCTCCAGCACC+AGG 0.556130 3_2:+23409294 Msa0399810:CDS
CGGATCTGTTGCAAGTTCTG+TGG 0.613339 3_2:+23409335 Msa0399810:CDS
AAAGAACAACTTCATTGTGG+TGG 0.635652 3_2:-23409223 Msa0399800:CDS
ACCAAAGAACAACTTCATTG+TGG 0.652153 3_2:-23409226 Msa0399800:CDS
CCTGGTGCTGGAGCTAACGA+CGG 0.671877 3_2:-23409294 Msa0399800:intron
GTCTTGGGCCGAGACTACAG+CGG 0.726320 3_2:-23409265 Msa0399800:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGAACAACTTCATTGTGG+TGG - chr3_2:23409226-23409245 Msa0399800:CDS 35.0%
ACCACAATGAAGTTGTTCTT+TGG + chr3_2:23409225-23409244 Msa0399810:CDS 35.0%
ACCAAAGAACAACTTCATTG+TGG - chr3_2:23409229-23409248 Msa0399800:CDS 35.0%
!! TAACGACGGACTAAGGTCTT+GGG - chr3_2:23409283-23409302 Msa0399800:intron 45.0%
CACAGAACTTGCAACAGATC+CGG - chr3_2:23409337-23409356 Msa0399800:intron 45.0%
GCAAGTTCTGTGGCTATGAT+TGG + chr3_2:23409345-23409364 Msa0399810:CDS 45.0%
!! CTAACGACGGACTAAGGTCT+TGG - chr3_2:23409284-23409303 Msa0399800:intron 50.0%
CGGATCTGTTGCAAGTTCTG+TGG + chr3_2:23409335-23409354 Msa0399810:CDS 50.0%
!! CTGGAGCTAACGACGGACTA+AGG - chr3_2:23409290-23409309 Msa0399800:intron 55.0%
CGTCTTCGCCGCTGTAGTCT+CGG + chr3_2:23409257-23409276 Msa0399810:CDS 60.0%
GTCTTGGGCCGAGACTACAG+CGG - chr3_2:23409268-23409287 Msa0399800:intron 60.0%
! CCTGGTGCTGGAGCTAACGA+CGG - chr3_2:23409297-23409316 Msa0399800:intron 60.0%
CCGTCGTTAGCTCCAGCACC+AGG + chr3_2:23409294-23409313 Msa0399810:CDS 65.0%
CCAGCATCAGGACCTGGTGC+TGG - chr3_2:23409309-23409328 Msa0399800:intron 65.0%
CCAGCACCAGGTCCTGATGC+TGG + chr3_2:23409306-23409325 Msa0399810:CDS 65.0%
GCAGCTCCAGCATCAGGACC+TGG - chr3_2:23409315-23409334 Msa0399800:intron 65.0%
GGTCCTGATGCTGGAGCTGC+CGG + chr3_2:23409315-23409334 Msa0399810:CDS 65.0%
GATCCGGCAGCTCCAGCATC+AGG - chr3_2:23409321-23409340 Msa0399800:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 23409204 23409410 23409204 ID=Msa0399810;Name=Msa0399810
chr3_2 mRNA 23409204 23409410 23409204 ID=Msa0399810-mRNA-1;Parent=Msa0399810;Name=Msa0399810-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|68
chr3_2 exon 23409204 23409410 23409204 ID=Msa0399810-mRNA-1:exon:4969;Parent=Msa0399810-mRNA-1
chr3_2 CDS 23409204 23409410 23409204 ID=Msa0399810-mRNA-1:cds;Parent=Msa0399810-mRNA-1
Gene Sequence

>Msa0399810

ATGGCATCATCATCATCAGCCACCACAATGAAGTTGTTCTTTGGTTTTGTCATCGTCTTCGCCGCTGTAGTCTCGGCCCAAGACCTTAGTCCGTCGTTAGCTCCAGCACCAGGTCCTGATGCTGGAGCTGCCGGATCTGTTGCAAGTTCTGTGGCTATGATTGGAGCTTCAGTTGTGCTGTCAATGTTTGCTATCTTCAAGAACTGA

Protein sequence

>Msa0399810

MASSSSATTMKLFFGFVIVFAAVVSAQDLSPSLAPAPGPDAGAAGSVASSVAMIGASVVLSMFAIFKN*