AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75560.t1 MTR_3g086050 94.118 221 12 1 1 221 1 220 1.94e-135 380
MS.gene75560.t1 MTR_3g052760 42.222 225 102 9 10 221 25 234 2.57e-53 172
MS.gene75560.t1 MTR_3g092460 38.994 159 77 4 64 221 95 234 3.82e-31 115
MS.gene75560.t1 MTR_3g092460 39.103 156 75 4 67 221 129 265 1.01e-29 112
MS.gene75560.t1 MTR_0004s0510 31.395 172 100 4 38 208 41 195 1.24e-20 87.0
MS.gene75560.t1 MTR_0004s0510 32.353 170 92 5 45 208 42 194 5.87e-20 85.5
MS.gene75560.t1 MTR_7g102590 43.590 78 38 2 132 208 40 112 7.37e-13 64.3
MS.gene75560.t1 MTR_4g088445 33.696 92 58 2 124 212 38 129 5.48e-12 62.0
MS.gene75560.t1 MTR_5g081530 29.365 126 72 4 91 207 17 134 6.68e-12 62.0
MS.gene75560.t1 MTR_4g010320 34.513 113 64 2 119 221 118 230 9.60e-12 63.2
MS.gene75560.t1 MTR_6g022000 34.314 102 61 4 119 214 28 129 4.06e-11 59.7
MS.gene75560.t1 MTR_6g061940 28.037 107 72 2 118 221 53 157 9.43e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75560.t1 AT4G27670 41.143 175 74 7 56 221 72 226 1.31e-35 127
MS.gene75560.t1 AT5G51440 33.333 177 97 5 33 208 39 195 1.39e-19 84.3
MS.gene75560.t1 AT1G52560 44.565 92 47 3 122 209 121 212 3.32e-19 83.6
MS.gene75560.t1 AT1G52560 44.565 92 47 3 122 209 128 219 4.50e-19 83.6
MS.gene75560.t1 AT4G25200 33.803 142 79 4 68 208 68 195 1.73e-17 78.6
MS.gene75560.t1 AT1G07400 31.783 129 72 6 91 209 19 141 2.36e-12 63.5
MS.gene75560.t1 AT5G37670 36.792 106 58 4 125 221 28 133 6.80e-12 62.0
MS.gene75560.t1 AT1G53540 32.673 101 65 2 124 221 55 155 3.49e-11 60.5
MS.gene75560.t1 AT1G59860 34.951 103 60 4 117 215 46 145 5.78e-11 59.7
MS.gene75560.t1 AT1G54050 33.333 117 63 4 104 206 22 137 5.90e-11 59.7
MS.gene75560.t1 AT1G54050 33.333 117 63 4 104 206 22 137 5.90e-11 59.7

Find 68 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCATCATTCTTCTTCT+TGG 0.080906 3.1:-68996749 None:intergenic
CTGCTGCACCGATAGGGTTA+TGG 0.285020 3.1:+68996452 MS.gene75560:CDS
AAGGAGTAGGTGGGTATAGA+AGG 0.294040 3.1:+68996590 MS.gene75560:CDS
ACAAAGAGGATGTTAAAGTT+TGG 0.296691 3.1:+68996686 MS.gene75560:CDS
GTGAGGAAGCAGCTCTATTA+TGG 0.329749 3.1:+68997052 MS.gene75560:CDS
TGTGCAGTTTGAGAATATTA+AGG 0.336710 3.1:+68996828 MS.gene75560:CDS
TTCTCAAACTGCACATTCTC+TGG 0.351423 3.1:-68996820 None:intergenic
TGCTGCACCGATAGGGTTAT+GGG 0.365142 3.1:+68996453 MS.gene75560:CDS
CAAAGAGGATGTTAAAGTTT+GGG 0.367241 3.1:+68996687 MS.gene75560:CDS
TTCTCTTGCTTCACCTGTCA+TGG 0.368553 3.1:-68996359 None:intergenic
GAATATTAAGGCTGAGGTTA+AGG 0.368713 3.1:+68996840 MS.gene75560:CDS
CACTCTTTATCCTCAAATCT+TGG 0.368943 3.1:+68996274 MS.gene75560:CDS
AACATTAAGATCAAGGACTT+TGG 0.390389 3.1:-68996908 None:intergenic
AGTTTGTCTTGATGAACTCT+TGG 0.419007 3.1:-68996317 None:intergenic
TGATGAACTCTTGGAAATCA+TGG 0.438584 3.1:-68996308 None:intergenic
GTCATTGAACATTAAGATCA+AGG 0.444311 3.1:-68996915 None:intergenic
GAGGGAAGTCACTTATGTTA+TGG 0.453824 3.1:+68997074 MS.gene75560:CDS
ATTAAGGCTGAGGTTAAGGA+TGG 0.462230 3.1:+68996844 MS.gene75560:CDS
TCATCGCCGCTACCGATTGA+AGG 0.462817 3.1:+68996571 MS.gene75560:CDS
TCTCCTTCTTTAACCTCCCA+TGG 0.463067 3.1:-68996625 None:intergenic
CTCTCTTCTTGGGTTCGGAC+TGG 0.467954 3.1:-68996423 None:intergenic
GCAAGAGATGATGGAAAGAA+TGG 0.475376 3.1:+68996501 MS.gene75560:CDS
TCAATTGTACTCATTGCAAA+CGG 0.479546 3.1:-68996541 None:intergenic
CATAACCCTATCGGTGCAGC+AGG 0.490168 3.1:-68996451 None:intergenic
GAATAAGTAGTAGTAGCCTT+AGG 0.490783 3.1:-68996886 None:intergenic
CAATTGTACTCATTGCAAAC+GGG 0.496144 3.1:-68996540 None:intergenic
AAGGACAGTGCAAGAGATGA+TGG 0.500427 3.1:+68996492 MS.gene75560:CDS
TCAATCGGTAGCGGCGATGA+AGG 0.505690 3.1:-68996568 None:intergenic
CTTCGAGTCAAAGCCATGAC+AGG 0.510747 3.1:+68996346 MS.gene75560:CDS
GTTTGAGAATATTAAGGCTG+AGG 0.515148 3.1:+68996834 MS.gene75560:CDS
AGAATGATGAAGATGAAGAA+TGG 0.516116 3.1:+68996758 MS.gene75560:CDS
TCTTTACATAACCATTCCTA+AGG 0.516609 3.1:+68996870 MS.gene75560:CDS
AGTAGGTGGGTATAGAAGGA+GGG 0.522121 3.1:+68996594 MS.gene75560:CDS
ACATCCTCTTTGTTCATACC+AGG 0.528873 3.1:-68996676 None:intergenic
GAGTAGGTGGGTATAGAAGG+AGG 0.533601 3.1:+68996593 MS.gene75560:CDS
CTGTCATGGCTTTGACTCGA+AGG 0.534564 3.1:-68996345 None:intergenic
TACCCACCTACTCCTTCAAT+CGG 0.537111 3.1:-68996583 None:intergenic
GATGTTTAAATCTGGTTGTG+AGG 0.537899 3.1:+68997035 MS.gene75560:intron
AACTCTTGGAAATCATGGAC+AGG 0.539369 3.1:-68996303 None:intergenic
TAAGGCTGAGGTTAAGGATG+GGG 0.545488 3.1:+68996846 MS.gene75560:CDS
GTAGGTGGGTATAGAAGGAG+GGG 0.548522 3.1:+68996595 MS.gene75560:CDS
GAGAAAGTGAATACAAGATG+AGG 0.559520 3.1:+68996644 MS.gene75560:CDS
TCTTCTTGGGTTCGGACTGG+TGG 0.560738 3.1:-68996420 None:intergenic
GGAAAGAATGGAAAGCATGA+TGG 0.561301 3.1:+68996513 MS.gene75560:CDS
GAAGAATGGTCTAAGAGCTA+TGG 0.561468 3.1:+68996772 MS.gene75560:CDS
AAGATGAGGTTTGATATGCC+TGG 0.574216 3.1:+68996658 MS.gene75560:CDS
ACACCATGGGAGGTTAAAGA+AGG 0.575898 3.1:+68996622 MS.gene75560:CDS
TTGGGTGGAAGAGAAGATGT+TGG 0.576341 3.1:+68996705 MS.gene75560:CDS
CCGCTACCGATTGAAGGAGT+AGG 0.578817 3.1:+68996577 MS.gene75560:CDS
AGAGGATGTTAAAGTTTGGG+TGG 0.581219 3.1:+68996690 MS.gene75560:CDS
CAGGTTAAGACCAAGATTTG+AGG 0.593235 3.1:-68996284 None:intergenic
TTAAGGCTGAGGTTAAGGAT+GGG 0.596993 3.1:+68996845 MS.gene75560:CDS
AATCGATCCCATAACCCTAT+CGG 0.598439 3.1:-68996460 None:intergenic
AGGAAGCAGCTCTATTATGG+AGG 0.602448 3.1:+68997055 MS.gene75560:CDS
TATTGTTTGATCTTGAAACA+TGG 0.608054 3.1:-68996390 None:intergenic
GAGGGGAAGAACACCATGGG+AGG 0.617779 3.1:+68996612 MS.gene75560:CDS
AGTAGTAGTAGCCTTAGGAA+TGG 0.626005 3.1:-68996881 None:intergenic
TTGCAATGAGTACAATTGAG+TGG 0.632366 3.1:+68996545 MS.gene75560:CDS
CTACCGATTGAAGGAGTAGG+TGG 0.639864 3.1:+68996580 MS.gene75560:CDS
TATACTCACATGTGAAGCAG+TGG 0.642490 3.1:-68997111 None:intergenic
GGGATCGATTTCTTACGACA+AGG 0.653086 3.1:+68996473 MS.gene75560:CDS
GGAAGCAGCTCTATTATGGA+GGG 0.658205 3.1:+68997056 MS.gene75560:CDS
AAGGAGGGGAAGAACACCAT+GGG 0.659587 3.1:+68996609 MS.gene75560:CDS
AATGGTCTAAGAGCTATGGT+AGG 0.662268 3.1:+68996776 MS.gene75560:CDS
CCTACTCCTTCAATCGGTAG+CGG 0.682306 3.1:-68996577 None:intergenic
TACCGATTGAAGGAGTAGGT+GGG 0.683545 3.1:+68996581 MS.gene75560:CDS
GAAGGAGGGGAAGAACACCA+TGG 0.687857 3.1:+68996608 MS.gene75560:CDS
TATGCCTGGTATGAACAAAG+AGG 0.732695 3.1:+68996672 MS.gene75560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAACATCTTACAAAAA+GGG - chr3.1:68997026-68997045 None:intergenic 15.0%
!! GATTTAAACATCTTACAAAA+AGG - chr3.1:68997027-68997046 None:intergenic 20.0%
!!! TTTTGTAAGATGTTTAAATC+TGG + chr3.1:68997027-68997046 MS.gene75560:intron 20.0%
! TATTGTTTGATCTTGAAACA+TGG - chr3.1:68996393-68996412 None:intergenic 25.0%
!!! AATGATTCATTTTTTTTCCC+TGG + chr3.1:68996938-68996957 MS.gene75560:intron 25.0%
AACATTAAGATCAAGGACTT+TGG - chr3.1:68996911-68996930 None:intergenic 30.0%
ACAAAGAGGATGTTAAAGTT+TGG + chr3.1:68996686-68996705 MS.gene75560:CDS 30.0%
AGAATGATGAAGATGAAGAA+TGG + chr3.1:68996758-68996777 MS.gene75560:CDS 30.0%
ATCTTCATCATTCTTCTTCT+TGG - chr3.1:68996752-68996771 None:intergenic 30.0%
CAAAGAGGATGTTAAAGTTT+GGG + chr3.1:68996687-68996706 MS.gene75560:CDS 30.0%
GTCATTGAACATTAAGATCA+AGG - chr3.1:68996918-68996937 None:intergenic 30.0%
TCAATTGTACTCATTGCAAA+CGG - chr3.1:68996544-68996563 None:intergenic 30.0%
TCTTTACATAACCATTCCTA+AGG + chr3.1:68996870-68996889 MS.gene75560:CDS 30.0%
TGTGCAGTTTGAGAATATTA+AGG + chr3.1:68996828-68996847 MS.gene75560:CDS 30.0%
AAAAACTCTCTTCTTGGGTT+CGG - chr3.1:68996431-68996450 None:intergenic 35.0%
AAAGCCTAATTAACAACTCC+AGG - chr3.1:68996959-68996978 None:intergenic 35.0%
AAGCCTAATTAACAACTCCA+GGG - chr3.1:68996958-68996977 None:intergenic 35.0%
AGTTTGTCTTGATGAACTCT+TGG - chr3.1:68996320-68996339 None:intergenic 35.0%
CAATTGTACTCATTGCAAAC+GGG - chr3.1:68996543-68996562 None:intergenic 35.0%
CACTCTTTATCCTCAAATCT+TGG + chr3.1:68996274-68996293 MS.gene75560:CDS 35.0%
GAATAAGTAGTAGTAGCCTT+AGG - chr3.1:68996889-68996908 None:intergenic 35.0%
GAATATTAAGGCTGAGGTTA+AGG + chr3.1:68996840-68996859 MS.gene75560:CDS 35.0%
GAGAAAGTGAATACAAGATG+AGG + chr3.1:68996644-68996663 MS.gene75560:CDS 35.0%
GATGTTTAAATCTGGTTGTG+AGG + chr3.1:68997035-68997054 MS.gene75560:intron 35.0%
GTTTGAGAATATTAAGGCTG+AGG + chr3.1:68996834-68996853 MS.gene75560:CDS 35.0%
TGATGAACTCTTGGAAATCA+TGG - chr3.1:68996311-68996330 None:intergenic 35.0%
TTGCAATGAGTACAATTGAG+TGG + chr3.1:68996545-68996564 MS.gene75560:CDS 35.0%
TTTCCCTGGAGTTGTTAATT+AGG + chr3.1:68996952-68996971 MS.gene75560:intron 35.0%
AACTCTTGGAAATCATGGAC+AGG - chr3.1:68996306-68996325 None:intergenic 40.0%
AAGATGAGGTTTGATATGCC+TGG + chr3.1:68996658-68996677 MS.gene75560:CDS 40.0%
AATCGATCCCATAACCCTAT+CGG - chr3.1:68996463-68996482 None:intergenic 40.0%
AATGGTCTAAGAGCTATGGT+AGG + chr3.1:68996776-68996795 MS.gene75560:CDS 40.0%
ACATCCTCTTTGTTCATACC+AGG - chr3.1:68996679-68996698 None:intergenic 40.0%
AGAGGATGTTAAAGTTTGGG+TGG + chr3.1:68996690-68996709 MS.gene75560:CDS 40.0%
AGCAGGAAAAACTCTCTTCT+TGG - chr3.1:68996437-68996456 None:intergenic 40.0%
ATTAAGGCTGAGGTTAAGGA+TGG + chr3.1:68996844-68996863 MS.gene75560:CDS 40.0%
CAGGTTAAGACCAAGATTTG+AGG - chr3.1:68996287-68996306 None:intergenic 40.0%
GAAGAATGGTCTAAGAGCTA+TGG + chr3.1:68996772-68996791 MS.gene75560:CDS 40.0%
GAGGGAAGTCACTTATGTTA+TGG + chr3.1:68997074-68997093 MS.gene75560:CDS 40.0%
GCAGGAAAAACTCTCTTCTT+GGG - chr3.1:68996436-68996455 None:intergenic 40.0%
TATGCCTGGTATGAACAAAG+AGG + chr3.1:68996672-68996691 MS.gene75560:CDS 40.0%
TTAAGGCTGAGGTTAAGGAT+GGG + chr3.1:68996845-68996864 MS.gene75560:CDS 40.0%
TTCTCAAACTGCACATTCTC+TGG - chr3.1:68996823-68996842 None:intergenic 40.0%
! AGTAGTAGTAGCCTTAGGAA+TGG - chr3.1:68996884-68996903 None:intergenic 40.0%
! GGAAAGAATGGAAAGCATGA+TGG + chr3.1:68996513-68996532 MS.gene75560:CDS 40.0%
!! GCAAGAGATGATGGAAAGAA+TGG + chr3.1:68996501-68996520 MS.gene75560:CDS 40.0%
AAGGACAGTGCAAGAGATGA+TGG + chr3.1:68996492-68996511 MS.gene75560:CDS 45.0%
AAGGAGTAGGTGGGTATAGA+AGG + chr3.1:68996590-68996609 MS.gene75560:CDS 45.0%
ACACCATGGGAGGTTAAAGA+AGG + chr3.1:68996622-68996641 MS.gene75560:CDS 45.0%
AGGAAGCAGCTCTATTATGG+AGG + chr3.1:68997055-68997074 MS.gene75560:CDS 45.0%
AGTAGGTGGGTATAGAAGGA+GGG + chr3.1:68996594-68996613 MS.gene75560:CDS 45.0%
GGAAGCAGCTCTATTATGGA+GGG + chr3.1:68997056-68997075 MS.gene75560:CDS 45.0%
GTGAGGAAGCAGCTCTATTA+TGG + chr3.1:68997052-68997071 MS.gene75560:CDS 45.0%
TAAGGCTGAGGTTAAGGATG+GGG + chr3.1:68996846-68996865 MS.gene75560:CDS 45.0%
TACCCACCTACTCCTTCAAT+CGG - chr3.1:68996586-68996605 None:intergenic 45.0%
TCTCCTTCTTTAACCTCCCA+TGG - chr3.1:68996628-68996647 None:intergenic 45.0%
TTCTCTTGCTTCACCTGTCA+TGG - chr3.1:68996362-68996381 None:intergenic 45.0%
TTGGGTGGAAGAGAAGATGT+TGG + chr3.1:68996705-68996724 MS.gene75560:CDS 45.0%
! GGGATCGATTTCTTACGACA+AGG + chr3.1:68996473-68996492 MS.gene75560:CDS 45.0%
! TACCGATTGAAGGAGTAGGT+GGG + chr3.1:68996581-68996600 MS.gene75560:CDS 45.0%
!! TTTTTCCTGCTGCACCGATA+GGG + chr3.1:68996446-68996465 MS.gene75560:CDS 45.0%
AAGGAGGGGAAGAACACCAT+GGG + chr3.1:68996609-68996628 MS.gene75560:CDS 50.0%
CCTACTCCTTCAATCGGTAG+CGG - chr3.1:68996580-68996599 None:intergenic 50.0%
CTTCGAGTCAAAGCCATGAC+AGG + chr3.1:68996346-68996365 MS.gene75560:CDS 50.0%
GAGTAGGTGGGTATAGAAGG+AGG + chr3.1:68996593-68996612 MS.gene75560:CDS 50.0%
GTAGGTGGGTATAGAAGGAG+GGG + chr3.1:68996595-68996614 MS.gene75560:CDS 50.0%
! CTACCGATTGAAGGAGTAGG+TGG + chr3.1:68996580-68996599 MS.gene75560:CDS 50.0%
! TGCTGCACCGATAGGGTTAT+GGG + chr3.1:68996453-68996472 MS.gene75560:CDS 50.0%
!! CTGTCATGGCTTTGACTCGA+AGG - chr3.1:68996348-68996367 None:intergenic 50.0%
!! GTTTTTCCTGCTGCACCGAT+AGG + chr3.1:68996445-68996464 MS.gene75560:CDS 50.0%
CATAACCCTATCGGTGCAGC+AGG - chr3.1:68996454-68996473 None:intergenic 55.0%
CTCTCTTCTTGGGTTCGGAC+TGG - chr3.1:68996426-68996445 None:intergenic 55.0%
GAAGGAGGGGAAGAACACCA+TGG + chr3.1:68996608-68996627 MS.gene75560:CDS 55.0%
! CCGCTACCGATTGAAGGAGT+AGG + chr3.1:68996577-68996596 MS.gene75560:CDS 55.0%
! CTGCTGCACCGATAGGGTTA+TGG + chr3.1:68996452-68996471 MS.gene75560:CDS 55.0%
! TCAATCGGTAGCGGCGATGA+AGG - chr3.1:68996571-68996590 None:intergenic 55.0%
! TCATCGCCGCTACCGATTGA+AGG + chr3.1:68996571-68996590 MS.gene75560:CDS 55.0%
! TCTTCTTGGGTTCGGACTGG+TGG - chr3.1:68996423-68996442 None:intergenic 55.0%
GAGGGGAAGAACACCATGGG+AGG + chr3.1:68996612-68996631 MS.gene75560:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 68996268 68997128 68996268 ID=MS.gene75560
chr3.1 mRNA 68996268 68997128 68996268 ID=MS.gene75560.t1;Parent=MS.gene75560
chr3.1 exon 68996268 68996927 68996268 ID=MS.gene75560.t1.exon1;Parent=MS.gene75560.t1
chr3.1 CDS 68996268 68996927 68996268 ID=cds.MS.gene75560.t1;Parent=MS.gene75560.t1
chr3.1 exon 68997036 68997128 68997036 ID=MS.gene75560.t1.exon2;Parent=MS.gene75560.t1
chr3.1 CDS 68997036 68997128 68997036 ID=cds.MS.gene75560.t1;Parent=MS.gene75560.t1
Gene Sequence

>MS.gene75560

ATGGCACACTCTTTATCCTCAAATCTTGGTCTTAACCTGTCCATGATTTCCAAGAGTTCATCAAGACAAACTCATTTCCTTCGAGTCAAAGCCATGACAGGTGAAGCAAGAGAAAAACTTGACCATGTTTCAAGATCAAACAATATTAAACACCACCAGTCCGAACCCAAGAAGAGAGTTTTTCCTGCTGCACCGATAGGGTTATGGGATCGATTTCTTACGACAAGGACAGTGCAAGAGATGATGGAAAGAATGGAAAGCATGATGGAAGACCCGTTTGCAATGAGTACAATTGAGTGGCCTTCATCGCCGCTACCGATTGAAGGAGTAGGTGGGTATAGAAGGAGGGGAAGAACACCATGGGAGGTTAAAGAAGGAGAAAGTGAATACAAGATGAGGTTTGATATGCCTGGTATGAACAAAGAGGATGTTAAAGTTTGGGTGGAAGAGAAGATGTTGGTTGTGAAAGCTGAGAAAGCATCCAAGAAGAAGAATGATGAAGATGAAGAATGGTCTAAGAGCTATGGTAGGTATAGTAGCAGAATTGCTTTTCCAGAGAATGTGCAGTTTGAGAATATTAAGGCTGAGGTTAAGGATGGGGTTCTTTACATAACCATTCCTAAGGCTACTACTACTTATTCCAAAGTCCTTGATCTTAATGTTCAATGACAATGATTCATTTTTTTTCCCTGGAGTTGTTAATTAGGCTTTTGTTATTTTTGATATATATTTAGATTTTTATATTGTTTTACGAACCCTTTTTGTAAGATGTTTAAATCTGGTTGTGAGGAAGCAGCTCTATTATGGAGGGAAGTCACTTATGTTATGGCGCAGCTGTCTCTGCCACTGCTTCACATGTGA

Protein sequence

>MS.gene75560.t1

MAHSLSSNLGLNLSMISKSSSRQTHFLRVKAMTGEAREKLDHVSRSNNIKHHQSEPKKRVFPAAPIGLWDRFLTTRTVQEMMERMESMMEDPFAMSTIEWPSSPLPIEGVGGYRRRGRTPWEVKEGESEYKMRFDMPGMNKEDVKVWVEEKMLVVKAEKASKKKNDEDEEWSKSYGRYSSRIAFPENVQFENIKAEVKDGVLYITIPKATTTYSKVLDLNMFKSGCEEAALLWREVTYVMAQLSLPLLHM