AlfalfaGEDB | Msa0068760

M. sativa cultivar zhongmu-4 / Msa0068760

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068760	XP_013467519.1	96.552	116	4	0	1	116	1	116	3.87e-70	215

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068760	A0A072VIW8	96.552	116	4	0	1	116	1	116	1.85e-70	215

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004060	Msa0068760	0.812264	4.626578e-51	-8.615850e-47
Msa0006880	Msa0068760	0.801386	9.228599e-49	-8.615850e-47
Msa0008090	Msa0068760	0.800249	1.574839e-48	-8.615850e-47
Msa0008410	Msa0068760	0.818453	1.944943e-52	-8.615850e-47
Msa0027580	Msa0068760	0.801563	8.486979e-49	-8.615850e-47
Msa0032260	Msa0068760	0.807835	4.161884e-50	-8.615850e-47
Msa0055150	Msa0068760	0.827276	1.711199e-54	-8.615850e-47
Msa0057140	Msa0068760	0.809169	2.161344e-50	-8.615850e-47
Msa0057710	Msa0068760	0.800518	1.388223e-48	-8.615850e-47
Msa0068760	Msa0076000	0.803465	3.440754e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068760	MtrunA17_Chr1g0172941	96.552	116	4	0	1	116	1	116	3.56e-74	215

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0068760	AT3G57320.1	50.000	96	47	1	8	102	5	100	2.92e-22	84.3

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Find 21 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAGAGAAGGAAGCAG+AGG	0.589194	1_2:+35983547	Msa0068760:five_prime_UTR
AATTATGTTAAATTAAATAT+AGG	0.336041	1_2:+35983470	None:intergenic
ACTTTGTCAGATACTGAGAG+TGG	0.633351	1_2:+35986239	Msa0068760:intron
ATCGCAGAAGATGAATTATA+CGG	0.472527	1_2:-35986457	None:intergenic
CCATATTGCTAAAACCAGCA+AGG	0.666565	1_2:-35987343	None:intergenic
CGAAATATGTTTGGCCTTGC+TGG	0.219767	1_2:+35987329	Msa0068760:three_prime_UTR
CTTTGTCAGATACTGAGAGT+GGG	0.589362	1_2:+35986240	Msa0068760:intron
GAATTTGTATCGAAGAATGG+TGG	0.607570	1_2:+35983569	Msa0068760:exon
GAGGAATTTGTATCGAAGAA+TGG	0.537737	1_2:+35983566	Msa0068760:exon
GAGTGGGGAACAATTGAAAG+TGG	0.620724	1_2:+35986256	Msa0068760:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTCTGTTAAAATAAACGAGT+TGG	+	chr1_2:35983593-35983612	Msa0068760:CDS	30.0%
AAAAGAATGAATAGTGACCT+CGG	-	chr1_2:35983658-35983677	None:intergenic	30.0%
TCGTATCACATCTATCAAAT+CGG	-	chr1_2:35983925-35983944	None:intergenic	30.0%
TCAATGAGTGTATGAAAAAG+TGG	+	chr1_2:35983987-35984006	Msa0068760:intron	30.0%
CAATGAGTGTATGAAAAAGT+GGG	+	chr1_2:35983988-35984007	Msa0068760:intron	30.0%
AATAGATGGAATGTTGAAGT+AGG	-	chr1_2:35984402-35984421	None:intergenic	30.0%
CAAAGGAAATAGAATGTGTA+GGG	-	chr1_2:35984998-35985017	None:intergenic	30.0%
TTCTGCTTCAAAGGAAAAAT+GGG	-	chr1_2:35985277-35985296	None:intergenic	30.0%
ATTCTGCTTCAAAGGAAAAA+TGG	-	chr1_2:35985278-35985297	None:intergenic	30.0%
ACTCATGAAAACTTGAGTTT+GGG	-	chr1_2:35985418-35985437	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	35983484	35987439	35983484	ID=Msa0068760;Name=Msa0068760
chr1_2	mRNA	35983484	35987439	35983484	ID=Msa0068760-mRNA-1;Parent=Msa0068760;Name=Msa0068760-mRNA-1;_AED=0.08;_eAED=0.08;_QI=101\|1\|1\|1\|1\|1\|5\|192\|116
chr1_2	exon	35983484	35983659	35983484	ID=Msa0068760-mRNA-1:exon:11455;Parent=Msa0068760-mRNA-1
chr1_2	exon	35986103	35986145	35986103	ID=Msa0068760-mRNA-1:exon:11456;Parent=Msa0068760-mRNA-1
chr1_2	exon	35986249	35986340	35986249	ID=Msa0068760-mRNA-1:exon:11457;Parent=Msa0068760-mRNA-1
chr1_2	exon	35986419	35986511	35986419	ID=Msa0068760-mRNA-1:exon:11458;Parent=Msa0068760-mRNA-1
chr1_2	exon	35987200	35987439	35987200	ID=Msa0068760-mRNA-1:exon:11459;Parent=Msa0068760-mRNA-1
chr1_2	five_prime_UTR	35983484	35983584	35983484	ID=Msa0068760-mRNA-1:five_prime_utr;Parent=Msa0068760-mRNA-1
chr1_2	CDS	35983585	35983659	35983585	ID=Msa0068760-mRNA-1:cds;Parent=Msa0068760-mRNA-1
chr1_2	CDS	35986103	35986145	35986103	ID=Msa0068760-mRNA-1:cds;Parent=Msa0068760-mRNA-1
chr1_2	CDS	35986249	35986340	35986249	ID=Msa0068760-mRNA-1:cds;Parent=Msa0068760-mRNA-1
chr1_2	CDS	35986419	35986511	35986419	ID=Msa0068760-mRNA-1:cds;Parent=Msa0068760-mRNA-1
chr1_2	CDS	35987200	35987247	35987200	ID=Msa0068760-mRNA-1:cds;Parent=Msa0068760-mRNA-1
chr1_2	three_prime_UTR	35987248	35987439	35987248	ID=Msa0068760-mRNA-1:three_prime_utr;Parent=Msa0068760-mRNA-1

Gene Sequence	>Msa0068760 ATGGTGGAGTCTGTTAAAATAAACGAGTTGGAAGTCACCTTAAAGCCTTTCTATCGAAGAGCTTCTGAAGCCGAGGAACGCTTATCTAGACTTGAAGCTGCCATCAATAGTAAAAAAGATACTGAGAGTGGGGAACAATTGAAAGTGGTTAATGATCTACAGTCAAAGCTAGAAGTTGCGAATGCTGAGTTAATTTCAGAAAAACGGAAGGCTCAGGTGCTTGCTGCAGAAAATGAAAAACTTCAATACCGTATAATTCATCTTCTGCGATCACTCAAGAATGCTGATCAGAAATTAGAGCAAGTCAAAGCACAGGAACAGCTGGAGAGCTTGAAATTGCAAGATTCTTAA
Protein sequence	>Msa0068760 MVESVKINELEVTLKPFYRRASEAEERLSRLEAAINSKKDTESGEQLKVVNDLQSKLEVANAELISEKRKAQVLAAENEKLQYRIIHLLRSLKNADQKLEQVKAQEQLESLKLQDS*