AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0175060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0175060 MtrunA17_Chr1g0201331 99.000 300 3 0 1 300 1 300 0.0 600
Msa0175060 MtrunA17_Chr4g0001221 59.028 288 103 2 9 287 7 288 1.26e-112 327
Msa0175060 MtrunA17_Chr2g0333771 56.908 304 111 6 13 300 11 310 1.41e-107 315
Msa0175060 MtrunA17_Chr4g0001111 54.378 217 84 2 9 216 7 217 1.23e-71 221
Msa0175060 MtrunA17_Chr8g0379901 49.362 235 108 3 69 300 34 260 1.36e-68 214
Msa0175060 MtrunA17_Chr8g0379911 48.511 235 110 3 69 300 33 259 1.82e-68 214
Msa0175060 MtrunA17_Chr2g0284581 47.600 250 112 4 54 300 26 259 1.08e-67 212
Msa0175060 MtrunA17_Chr2g0284631 50.673 223 108 2 62 283 80 301 7.63e-67 211
Msa0175060 MtrunA17_Chr2g0284591 48.718 234 109 3 70 300 32 257 1.51e-66 209
Msa0175060 MtrunA17_Chr2g0284611 48.696 230 113 2 74 300 38 265 2.26e-65 206
Msa0175060 MtrunA17_Chr7g0217331 44.292 219 122 0 72 290 35 253 9.76e-64 202
Msa0175060 MtrunA17_Chr2g0284601 49.057 212 97 3 92 300 8 211 7.27e-60 190
Msa0175060 MtrunA17_Chr5g0426131 40.074 272 136 5 28 294 6 255 1.77e-58 188
Msa0175060 MtrunA17_Chr4g0001161 62.857 140 52 0 87 226 1 140 2.24e-55 176
Msa0175060 MtrunA17_Chr1g0184541 43.111 225 128 0 69 293 57 281 3.60e-55 181
Msa0175060 MtrunA17_Chr5g0435051 39.921 253 138 3 32 283 10 249 3.32e-52 172
Msa0175060 MtrunA17_Chr4g0044201 41.949 236 111 5 70 300 31 245 8.71e-51 168
Msa0175060 MtrunA17_Chr3g0079421 41.004 239 135 4 47 283 1 235 3.48e-49 164
Msa0175060 MtrunA17_Chr4g0001151 59.542 131 38 1 87 217 1 116 3.84e-41 145
Msa0175060 MtrunA17_Chr2g0331261 39.189 222 114 7 69 275 15 230 1.15e-31 118
Msa0175060 MtrunA17_Chr4g0001121 64.835 91 24 2 216 300 10 98 3.06e-31 112
Msa0175060 MtrunA17_Chr1g0146161 35.841 226 126 7 74 287 19 237 6.83e-29 110
Msa0175060 MtrunA17_Chr8g0340161 37.089 213 113 7 78 275 23 229 2.18e-28 109
Msa0175060 MtrunA17_Chr1g0146171 35.981 214 118 7 74 275 19 225 4.51e-28 108
Msa0175060 MtrunA17_Chr1g0212671 33.333 228 132 7 78 291 25 246 3.19e-23 95.9
Msa0175060 MtrunA17_Chr5g0399341 33.333 234 134 5 78 298 23 247 6.97e-22 92.0
Msa0175060 MtrunA17_Chr2g0326621 31.076 251 138 7 71 287 37 286 5.99e-20 87.8
Msa0175060 MtrunA17_Chr4g0030861 32.870 216 123 8 79 279 26 234 1.52e-19 85.9
Msa0175060 MtrunA17_Chr7g0222791 31.330 233 130 8 78 291 25 246 3.96e-19 84.7
Msa0175060 MtrunA17_Chr6g0478191 28.571 224 149 4 78 293 25 245 1.68e-18 83.2
Msa0175060 MtrunA17_Chr7g0264961 30.804 224 144 6 78 292 25 246 1.56e-17 80.5
Msa0175060 MtrunA17_Chr1g0188771 30.804 224 144 5 78 293 25 245 1.97e-17 80.1
Msa0175060 MtrunA17_Chr3g0145671 29.487 234 147 7 66 282 29 261 1.04e-16 78.6
Msa0175060 MtrunA17_Chr4g0020781 33.516 182 101 5 126 299 36 205 6.26e-16 75.1
Msa0175060 MtrunA17_Chr8g0386961 29.646 226 139 8 78 285 56 279 4.10e-14 71.2
Msa0175060 MtrunA17_Chr7g0264111 27.490 251 156 8 71 299 36 282 4.60e-14 71.2
Msa0175060 MtrunA17_Chr4g0006001 29.333 225 140 9 78 285 60 282 4.74e-14 71.2
Msa0175060 MtrunA17_Chr3g0113211 26.577 222 146 6 78 282 59 280 8.68e-14 70.5
Msa0175060 MtrunA17_Chr5g0436701 27.111 225 146 6 78 285 59 282 1.94e-13 69.3
Msa0175060 MtrunA17_Chr5g0397391 28.319 226 142 8 78 285 56 279 2.52e-13 68.9
Msa0175060 MtrunA17_Chr4g0029071 25.197 254 158 7 66 287 31 284 7.12e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0175060 AT4G10380.1 70.819 281 72 3 25 300 29 304 8.38e-143 404
Msa0175060 AT1G80760.1 56.550 313 101 4 9 300 7 305 1.58e-110 323
Msa0175060 AT4G19030.1 49.167 240 108 3 69 300 50 283 3.50e-69 217
Msa0175060 AT5G37820.1 43.866 269 139 4 35 300 11 270 3.98e-68 214
Msa0175060 AT5G37820.2 43.866 269 139 4 35 300 47 306 1.28e-67 214
Msa0175060 AT5G37810.1 42.435 271 140 4 35 300 11 270 6.50e-67 211
Msa0175060 AT4G18910.1 46.694 242 111 3 69 300 47 280 4.94e-66 209
Msa0175060 AT1G31885.1 50.224 223 103 2 69 283 38 260 2.26e-64 205
Msa0175060 AT1G31885.2 49.029 206 97 2 86 283 1 206 5.52e-57 185
Msa0175060 AT2G34390.1 43.777 233 117 4 69 291 43 271 2.16e-52 174
Msa0175060 AT2G34390.2 43.172 227 115 4 75 291 7 229 1.62e-50 167
Msa0175060 AT3G06100.1 44.762 210 113 3 78 285 50 258 3.72e-47 159
Msa0175060 AT3G06100.2 44.340 212 115 3 78 287 2 212 3.97e-47 158
Msa0175060 AT4G19030.3 49.143 175 81 2 69 235 50 224 7.91e-46 154
Msa0175060 AT4G18910.2 48.315 178 80 2 69 236 47 222 8.65e-45 152
Msa0175060 AT4G19030.2 46.795 156 75 2 69 216 50 205 5.47e-35 126
Msa0175060 AT2G34390.3 41.573 178 91 3 69 236 43 217 9.76e-32 118
Msa0175060 AT3G16240.1 37.500 208 110 7 78 271 23 224 2.34e-29 112
Msa0175060 AT2G29870.1 50.980 102 49 1 190 291 22 122 5.46e-26 100
Msa0175060 AT2G34390.4 40.625 160 82 3 69 218 43 199 3.00e-25 101
Msa0175060 AT5G47450.1 36.652 221 121 7 69 275 14 229 4.77e-25 101
Msa0175060 AT4G17340.1 35.849 212 117 6 78 275 23 229 1.48e-24 99.8
Msa0175060 AT2G25810.1 36.842 209 113 7 79 275 24 225 1.59e-24 99.8
Msa0175060 AT3G26520.1 33.186 226 133 7 78 290 26 246 2.10e-22 94.0
Msa0175060 AT3G06100.3 39.855 138 82 1 149 285 40 177 3.32e-22 92.0
Msa0175060 AT2G36830.1 34.348 230 129 8 78 292 25 247 4.45e-22 93.2
Msa0175060 AT4G01470.1 31.760 233 129 8 78 291 25 246 1.18e-19 86.7
Msa0175060 AT1G17810.1 29.694 229 147 5 78 293 28 255 5.04e-18 82.4
Msa0175060 AT1G31885.3 43.878 98 55 0 69 166 38 135 7.07e-18 79.0
Msa0175060 AT4G35100.1 29.600 250 156 7 66 299 30 275 2.05e-17 80.9
Msa0175060 AT4G35100.2 29.600 250 156 7 66 299 30 275 2.05e-17 80.9
Msa0175060 AT2G16850.1 30.204 245 151 7 71 299 33 273 2.22e-16 77.8
Msa0175060 AT1G73190.1 28.509 228 147 5 78 292 28 252 3.28e-16 77.4
Msa0175060 AT3G53420.1 27.376 263 164 8 59 297 24 283 1.87e-15 75.5
Msa0175060 AT3G53420.2 27.376 263 164 8 59 297 24 283 1.87e-15 75.5
Msa0175060 AT5G60660.1 26.809 235 146 7 72 282 37 269 8.97e-15 73.6
Msa0175060 AT2G37170.1 27.490 251 155 8 71 297 34 281 3.33e-14 72.0
Msa0175060 AT2G37170.2 27.309 249 158 7 71 297 77 324 7.26e-14 71.2
Msa0175060 AT3G54820.1 27.556 225 139 7 71 272 35 258 2.92e-13 69.3
Msa0175060 AT2G37180.1 26.506 249 160 7 71 297 34 281 7.83e-13 67.8
Msa0175060 AT2G45960.1 28.444 225 142 8 78 285 56 278 3.49e-12 66.2
Msa0175060 AT1G01620.1 27.962 211 136 6 78 272 56 266 4.82e-12 65.5
Msa0175060 AT3G61430.2 28.000 225 143 8 78 285 56 278 5.16e-12 65.5
Msa0175060 AT3G61430.1 28.000 225 143 8 78 285 56 278 5.16e-12 65.5
Msa0175060 AT4G23400.1 28.000 225 143 8 78 285 57 279 7.67e-12 65.1
Msa0175060 AT4G00430.1 27.232 224 146 7 78 285 57 279 7.81e-12 65.1
Msa0175060 AT3G47440.1 27.897 233 141 6 78 296 27 246 2.70e-11 63.2
Msa0175060 AT1G01620.2 28.333 180 116 4 106 272 15 194 9.81e-11 60.8

Find 133 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGCATCTCTGGATATATT+TGG 0.216287 1_4:-60886159 Msa0175060:CDS
AATCTCTCCAACTTCGAAAT+TGG 0.246759 1_4:-60890867 Msa0175060:five_prime_UTR
GGTGTCCCTGACTCCGATTC+TGG 0.274084 1_4:+60890485 None:intergenic
TTGTATTTGTTGTTCACTAT+TGG 0.275818 1_4:+60887216 None:intergenic
ATAGATTATAAAATTGAGTT+TGG 0.281086 1_4:+60891026 None:intergenic
AAAGAGTACAGTTACATAAT+TGG 0.297226 1_4:+60890657 None:intergenic
TTCGAAGTTGGAGAGATTAT+AGG 0.312909 1_4:+60890872 None:intergenic
CAATGGAGTAGAAACTCTTA+TGG 0.319532 1_4:-60887196 Msa0175060:CDS
CTTGGTGCTCTAGCTGGTTC+TGG 0.336743 1_4:-60886125 Msa0175060:CDS
ATAAATTTGTTTCTAAGGTA+TGG 0.361143 1_4:+60890689 None:intergenic
TTGCTCAACATTCTTATCTC+AGG 0.361959 1_4:-60886602 Msa0175060:CDS
GAACATGTGCCCACGGGAAA+TGG 0.378646 1_4:+60887064 None:intergenic
ATGAATCCGGTGCGCACCTT+AGG 0.389490 1_4:-60886206 Msa0175060:CDS
GTTTACCATCCTTTCCTCTC+TGG 0.391500 1_4:-60886982 Msa0175060:CDS
TGCAATGCAGGTTGGTGAAT+TGG 0.400837 1_4:-60886651 Msa0175060:intron
TATGTAGTTGTTGTTAAGGT+TGG 0.402677 1_4:+60890807 None:intergenic
ATGTTCATTATTCTATCAAT+TGG 0.408502 1_4:-60887144 Msa0175060:CDS
ATCACTCACCATTGCCTTCG+CGG 0.410120 1_4:-60887094 Msa0175060:CDS
TAAGGTGCGCACCGGATTCA+TGG 0.410875 1_4:+60886208 None:intergenic
CAGGAAATTACAAGCATCTC+TGG 0.418374 1_4:-60886169 Msa0175060:CDS
TAATTTCCTGCTGCAACGGC+TGG 0.421426 1_4:+60886182 None:intergenic
CCGACGCTTGGTGCTCTAGC+TGG 0.422426 1_4:-60886131 Msa0175060:CDS
AAAATGCCAGAATCGGAGTC+AGG 0.425265 1_4:-60890491 Msa0175060:CDS
AATGGCCCTCCGGGAGTATC+CGG 0.425506 1_4:+60890437 None:intergenic
TGCATCGCTAATTTCTCTGC+TGG 0.426344 1_4:-60890320 Msa0175060:CDS
TTTGGAAAGTGCTATGAATA+TGG 0.427152 1_4:+60891044 None:intergenic
TGGATATGTAGTTGTTGTTA+AGG 0.429772 1_4:+60890803 None:intergenic
ATTCTTATAGATGAGAATAA+TGG 0.431504 1_4:+60890419 None:intergenic
TAATGAACATATGAAAAGAT+TGG 0.431700 1_4:+60890783 None:intergenic
CGGAGCTGAACCACCTCAAC+CGG 0.432157 1_4:-60886072 Msa0175060:CDS
TGCTGCAACGGCTGGACCTA+AGG 0.434795 1_4:+60886190 None:intergenic
TCAACAACTTATGGGGATGA+TGG 0.436919 1_4:+60890961 None:intergenic
AAGATGAGGTATTTAATTGT+GGG 0.439925 1_4:+60890750 None:intergenic
CAACGTGAGTCATTTGCAAT+GGG 0.443310 1_4:-60890383 Msa0175060:CDS
CGTTATCACGAAGCTTGATA+AGG 0.443454 1_4:+60886093 None:intergenic
TTCATGGAACCACCGCTTGT+TGG 0.443747 1_4:+60886224 None:intergenic
AAAGATGAGGTATTTAATTG+TGG 0.444642 1_4:+60890749 None:intergenic
CTATCAATTGGTCATATCTC+AGG 0.449732 1_4:-60887132 Msa0175060:CDS
TTGTGATCCAATTTCGAAGT+TGG 0.460953 1_4:+60890860 None:intergenic
CTTATCAAGCTTCGTGATAA+CGG 0.463299 1_4:-60886092 Msa0175060:CDS
GGAGCTTCCGTCGCTAGAGC+TGG 0.463603 1_4:-60886046 Msa0175060:exon
ATGGGAAATGCAGCTTGTGC+TGG 0.464614 1_4:-60887177 Msa0175060:CDS
TAAGAGAAAACTTGTGATGA+AGG 0.468893 1_4:+60890837 None:intergenic
AATGGAGTAGAAACTCTTAT+GGG 0.469616 1_4:-60887195 Msa0175060:CDS
CTAAGGTATGGAAAGAAAGA+AGG 0.477970 1_4:+60890701 None:intergenic
GTTATCACGAAGCTTGATAA+GGG 0.481400 1_4:+60886094 None:intergenic
CTGAACCACCTCAACCGGTT+AGG 0.481691 1_4:-60886067 Msa0175060:CDS
TAGCGACGGAAGCTCCTAAC+CGG 0.489899 1_4:+60886053 None:intergenic
TGTGTCATGAAAGCTTTGTT+TGG 0.490520 1_4:-60885881 Msa0175060:three_prime_UTR
CTTGTAATTTCCTGCTGCAA+CGG 0.491803 1_4:+60886178 None:intergenic
AGCCTAATTAAATAAAATGT+GGG 0.492607 1_4:-60885961 Msa0175060:three_prime_UTR
CGGCGTTTCGCCATTTCCCG+TGG 0.493217 1_4:-60887074 Msa0175060:CDS
GAGGTGCAACATGTGTATGC+CGG 0.494142 1_4:-60890360 Msa0175060:CDS
ACAGATTGGAGCAGAGTTTG+TGG 0.494648 1_4:-60887274 Msa0175060:intron
TATTTGGTGGCACCGACGCT+TGG 0.495981 1_4:-60886143 Msa0175060:CDS
TTAGGTCCAGCCGTTGCAGC+AGG 0.497485 1_4:-60886188 Msa0175060:CDS
CGGAAGCTCCTAACCGGTTG+AGG 0.497802 1_4:+60886059 None:intergenic
AAGCGGTGGTTCCATGAATC+CGG 0.497866 1_4:-60886219 Msa0175060:CDS
TACCATCCTTTCCTCTCTGG+TGG 0.500750 1_4:-60886979 Msa0175060:CDS
TCTAGCTAAATCTACAAACC+TGG 0.501272 1_4:-60890727 Msa0175060:five_prime_UTR
TTCACACACACTCGATTACA+AGG 0.504768 1_4:+60885934 None:intergenic
TGCTCAACATTCTTATCTCA+GGG 0.505305 1_4:-60886601 Msa0175060:intron
AGCGTCGGTGCCGGCGACAC+CGG 0.511138 1_4:-60890456 Msa0175060:CDS
AGGTGCAACATGTGTATGCC+GGG 0.512535 1_4:-60890359 Msa0175060:CDS
CAGATTGGAGCAGAGTTTGT+GGG 0.519331 1_4:-60887273 Msa0175060:CDS
GCCATGGTTTGTATTATGAA+TGG 0.521934 1_4:-60885827 Msa0175060:three_prime_UTR
GAATTGGCTGGTATTGCTGT+TGG 0.524769 1_4:-60886635 Msa0175060:CDS
GATGAGAATAATGGCCCTCC+GGG 0.524874 1_4:+60890428 None:intergenic
AGATGAGAATAATGGCCCTC+CGG 0.525358 1_4:+60890427 None:intergenic
AAAGCTTTGTTTGGTTAATG+TGG 0.526294 1_4:-60885872 Msa0175060:three_prime_UTR
TAATGGCAAGATTCTAGCCA+TGG 0.528152 1_4:-60885843 Msa0175060:three_prime_UTR
CATTTAGAAAATTATGTAGA+GGG 0.534464 1_4:-60890581 Msa0175060:five_prime_UTR
AAGTGTCTCTCTTTCTCAGA+AGG 0.536928 1_4:-60890285 Msa0175060:intron
CCGGGAGTATCCGGTGTCGC+CGG 0.538656 1_4:+60890446 None:intergenic
AGTTGTTGTTAAGGTTGGAA+TGG 0.541398 1_4:+60890812 None:intergenic
ACCGACGGCAGCGTCGGTGC+CGG 0.542854 1_4:-60890465 Msa0175060:CDS
GAGCCTAATTAAATAAAATG+TGG 0.546465 1_4:-60885962 Msa0175060:three_prime_UTR
TCAACGTGAGTCATTTGCAA+TGG 0.548537 1_4:-60890384 Msa0175060:CDS
TGCGCTTGTTATGCGCTCAA+AGG 0.549531 1_4:-60887006 Msa0175060:CDS
TAGATTTAGCTAGAAAGATG+AGG 0.551425 1_4:+60890736 None:intergenic
AAGGTGGATGAGAGGACTAA+TGG 0.556098 1_4:+60890922 None:intergenic
AGTACAGTTACATAATTGGA+TGG 0.558005 1_4:+60890661 None:intergenic
GGCGTTTCGCCATTTCCCGT+GGG 0.558137 1_4:-60887073 Msa0175060:CDS
TAATAATGTGTTCTGTTGTG+TGG 0.558749 1_4:-60885791 Msa0175060:three_prime_UTR
GAGCTTGAAATATGAGAAGT+GGG 0.558919 1_4:+60886001 None:intergenic
GCGAAACGCCGCGAAGGCAA+TGG 0.561703 1_4:+60887086 None:intergenic
TTCTGAGAAAGAGAGACACT+TGG 0.567115 1_4:+60890287 None:intergenic
GCTTGTCCAACGCTAACAGT+AGG 0.567496 1_4:+60886946 None:intergenic
GCCGGCACCGACGCTGCCGT+CGG 0.571291 1_4:+60890464 None:intergenic
CTAATATATGCAGCAACAGC+AGG 0.571804 1_4:-60887240 Msa0175060:CDS
AGAGACTTGTGCTACAATGT+AGG 0.572806 1_4:+60887038 None:intergenic
AAATGCCAGAATCGGAGTCA+GGG 0.577507 1_4:-60890490 Msa0175060:CDS
ACTTGTGCTACAATGTAGGC+TGG 0.578393 1_4:+60887042 None:intergenic
CCGGCGACACCGGATACTCC+CGG 0.580871 1_4:-60890446 Msa0175060:CDS
TAAGGTATGGAAAGAAAGAA+GGG 0.581499 1_4:+60890702 None:intergenic
CTTGTCCAACGCTAACAGTA+GGG 0.582924 1_4:+60886947 None:intergenic
GCGAAGGCAATGGTGAGTGA+TGG 0.584999 1_4:+60887096 None:intergenic
GCATTTAGAAAATTATGTAG+AGG 0.586537 1_4:-60890582 Msa0175060:five_prime_UTR
AGAGCTTGAAATATGAGAAG+TGG 0.588128 1_4:+60886000 None:intergenic
GCATCTCTGGATATATTTGG+TGG 0.589329 1_4:-60886156 Msa0175060:CDS
CTAGCTAAATCTACAAACCT+GGG 0.589533 1_4:-60890726 Msa0175060:five_prime_UTR
GAAATGGCGAAACGCCGCGA+AGG 0.592464 1_4:+60887080 None:intergenic
ACCATTCATAATACAAACCA+TGG 0.595763 1_4:+60885826 None:intergenic
ATGATGGAGACGAAGTGATG+AGG 0.595924 1_4:+60890977 None:intergenic
ATGCAGGTTGGTGAATTGGC+TGG 0.597369 1_4:-60886647 Msa0175060:intron
ATGAATTGAACGTACCGCAC+GGG 0.604011 1_4:+60886858 None:intergenic
CGTGGTTGAAGGTGGATGAG+AGG 0.608502 1_4:+60890914 None:intergenic
GCTGGACCTAAGGTGCGCAC+CGG 0.614709 1_4:+60886200 None:intergenic
TAGGCTGGAACATGTGCCCA+CGG 0.616186 1_4:+60887057 None:intergenic
ACTGTCCCTACTGTTAGCGT+TGG 0.619097 1_4:-60886952 Msa0175060:CDS
GTCGCAGCCAGCTCTAGCGA+CGG 0.626232 1_4:+60886039 None:intergenic
ATAGTGAACAACAAATACAA+TGG 0.626790 1_4:-60887213 Msa0175060:CDS
CCAGCTAGAGCACCAAGCGT+CGG 0.629619 1_4:+60886131 None:intergenic
AGGCTGGAACATGTGCCCAC+GGG 0.634760 1_4:+60887058 None:intergenic
CACCACCAGAGAGGAAAGGA+TGG 0.636860 1_4:+60886977 None:intergenic
AGGGACACCGACGGCAGCGT+CGG 0.638264 1_4:-60890471 Msa0175060:CDS
GCTTGAAATATGAGAAGTGG+GGG 0.639472 1_4:+60886003 None:intergenic
GTGAGTCATTTGCAATGGGG+AGG 0.644380 1_4:-60890379 Msa0175060:CDS
GCCTAATTAAATAAAATGTG+GGG 0.644829 1_4:-60885960 Msa0175060:three_prime_UTR
GTGACACCACCAGAGAGGAA+AGG 0.645575 1_4:+60886973 None:intergenic
TGGAAAGAAAGAAGGGACCC+AGG 0.648546 1_4:+60890709 None:intergenic
TGGATGAGAGGACTAATGGT+CGG 0.655282 1_4:+60890926 None:intergenic
AAGCTCCTAACCGGTTGAGG+TGG 0.655759 1_4:+60886062 None:intergenic
AATGCTAGGCCAACAAGCGG+TGG 0.661522 1_4:-60886233 Msa0175060:intron
AGCTTGAAATATGAGAAGTG+GGG 0.669139 1_4:+60886002 None:intergenic
ATCGGAGTCAGGGACACCGA+CGG 0.669278 1_4:-60890480 Msa0175060:CDS
GTAAATGCTAGGCCAACAAG+CGG 0.699062 1_4:-60886236 Msa0175060:intron
CGACACCGGATACTCCCGGA+GGG 0.703914 1_4:-60890442 Msa0175060:CDS
AACGTGAGTCATTTGCAATG+GGG 0.719998 1_4:-60890382 Msa0175060:CDS
GGACAGTGACACCACCAGAG+AGG 0.725765 1_4:+60886968 None:intergenic
GCGACACCGGATACTCCCGG+AGG 0.726349 1_4:-60890443 Msa0175060:CDS
TGCAACATGTGTATGCCGGG+TGG 0.746549 1_4:-60890356 Msa0175060:CDS
TGTTGCTACTGATTCCCGTG+CGG 0.778451 1_4:-60886872 Msa0175060:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAAATTTGTTTCTA+AGG + chr1_4:60886187-60886206 None:intergenic 10.0%
!! TAGTTAATTTAATTAACAAT+TGG - chr1_4:60886890-60886909 Msa0175060:CDS 10.0%
!! AGTTAATTTAATTAACAATT+GGG - chr1_4:60886891-60886910 Msa0175060:CDS 10.0%
!!! TAATAAAAAAATTGTTTTCA+AGG + chr1_4:60887268-60887287 None:intergenic 10.0%
!! TTATATTGAAATGAAATATA+TGG - chr1_4:60887491-60887510 Msa0175060:intron 10.0%
!!! ATTTCAATATATAATTTTGT+CGG - chr1_4:60887543-60887562 Msa0175060:intron 10.0%
!! TAAAATAAAAAAGATTAATG+CGG - chr1_4:60887933-60887952 Msa0175060:intron 10.0%
!!! TACTTTTCTTTTTTAAAATA+CGG + chr1_4:60888412-60888431 None:intergenic 10.0%
!! ATAGATTATAAAATTGAGTT+TGG + chr1_4:60885845-60885864 None:intergenic 15.0%
!!! AAATAGATAGCATTTTTTTT+AGG - chr1_4:60886797-60886816 Msa0175060:intron 15.0%
!!! AATAGATAGCATTTTTTTTA+GGG - chr1_4:60886798-60886817 Msa0175060:intron 15.0%
!!! GAATGTATATTTTTTTTTGA+AGG + chr1_4:60887182-60887201 None:intergenic 15.0%
!! AAAACTATTCAATATACTTT+CGG + chr1_4:60887831-60887850 None:intergenic 15.0%
!! CAAATATAGATAAAACTTAT+TGG - chr1_4:60888085-60888104 Msa0175060:intron 15.0%
!!! TTAAATGCACAAAATTTTAT+AGG - chr1_4:60888147-60888166 Msa0175060:intron 15.0%
!!! TAAAAAAAGTTGATTAAATG+AGG - chr1_4:60888522-60888541 Msa0175060:intron 15.0%
!!! AAAAAAAGTTGATTAAATGA+GGG - chr1_4:60888523-60888542 Msa0175060:intron 15.0%
!!! GAAATTACATAGATTTTTTT+TGG - chr1_4:60888993-60889012 Msa0175060:intron 15.0%
!!! TATAAGTTGTTTGTTTTTTT+TGG + chr1_4:60889151-60889170 None:intergenic 15.0%
!! ATTCAGACAAAATTTATATT+CGG + chr1_4:60890468-60890487 None:intergenic 15.0%
!! TAATGAACATATGAAAAGAT+TGG + chr1_4:60886088-60886107 None:intergenic 20.0%
!!! ATAAATTTGTTTCTAAGGTA+TGG + chr1_4:60886182-60886201 None:intergenic 20.0%
!!! TTTTGTTTTTGAGAGTTTTA+AGG + chr1_4:60886240-60886259 None:intergenic 20.0%
!! CATTTAGAAAATTATGTAGA+GGG - chr1_4:60886287-60886306 Msa0175060:intron 20.0%
!! TAAAAAAAAAATGCCAGAAT+CGG - chr1_4:60886370-60886389 Msa0175060:intron 20.0%
!! ATTCTTATAGATGAGAATAA+TGG + chr1_4:60886452-60886471 None:intergenic 20.0%
!!! TCTATTTGATATTTTAGTCA+AGG + chr1_4:60886784-60886803 None:intergenic 20.0%
!! ATTACAAATACAAAAACAAG+TGG + chr1_4:60886922-60886941 None:intergenic 20.0%
!!! AGGAAATAAATTTTATTTGC+TGG + chr1_4:60887204-60887223 None:intergenic 20.0%
!!! TTTTTGTTTGCTTCTTTTTT+AGG + chr1_4:60887224-60887243 None:intergenic 20.0%
!! TACATATACTATATTACACA+CGG + chr1_4:60887301-60887320 None:intergenic 20.0%
!!! AAATGTATTTTGACATAGAT+GGG - chr1_4:60887571-60887590 Msa0175060:intron 20.0%
!! TTTCATTGATAAACAAAAGA+TGG - chr1_4:60887611-60887630 Msa0175060:intron 20.0%
!!! ACATGTTTGATATTTTCTTA+GGG + chr1_4:60888480-60888499 None:intergenic 20.0%
!!! AACATGTTTGATATTTTCTT+AGG + chr1_4:60888481-60888500 None:intergenic 20.0%
!!! AAAAAAGTTGATTAAATGAG+GGG - chr1_4:60888524-60888543 Msa0175060:intron 20.0%
!! AAACTCAAAGTTTAAGTATT+CGG + chr1_4:60888574-60888593 None:intergenic 20.0%
!!! TAGGATTTATTTTTTTTGAG+AGG - chr1_4:60888663-60888682 Msa0175060:intron 20.0%
!!! GTTCGACTTTTTTTTTTTAA+GGG - chr1_4:60888693-60888712 Msa0175060:intron 20.0%
!! ATTGTTTAAACAACTAAAAG+TGG + chr1_4:60888887-60888906 None:intergenic 20.0%
!! ACAAAATTAAATCAACTACA+TGG + chr1_4:60889235-60889254 None:intergenic 20.0%
!!! TGATTTAATTTTGTAAGCTT+TGG - chr1_4:60889241-60889260 Msa0175060:intron 20.0%
!!! GATTTAATTTTGTAAGCTTT+GGG - chr1_4:60889242-60889261 Msa0175060:intron 20.0%
!!! ATTTAATTTTGTAAGCTTTG+GGG - chr1_4:60889243-60889262 Msa0175060:intron 20.0%
!!! TTTTTCCAAAAATCTACATT+TGG - chr1_4:60889409-60889428 Msa0175060:intron 20.0%
!!! TACTTTTCCTTTTTCATTAA+GGG + chr1_4:60889539-60889558 None:intergenic 20.0%
!!! TTACTTTTCCTTTTTCATTA+AGG + chr1_4:60889540-60889559 None:intergenic 20.0%
!! ATGTTCATTATTCTATCAAT+TGG - chr1_4:60889724-60889743 Msa0175060:intron 20.0%
!!! AAATTTCAAGTTCTCTAAAT+TGG - chr1_4:60890110-60890129 Msa0175060:intron 20.0%
!!! AATTTCAAGTTCTCTAAATT+GGG - chr1_4:60890111-60890130 Msa0175060:intron 20.0%
!!! ATTGAGTTTTTTATGAGTTA+AGG - chr1_4:60890162-60890181 Msa0175060:intron 20.0%
!!! TGATGTTATTGATTTGTATT+TGG - chr1_4:60890312-60890331 Msa0175060:CDS 20.0%
!!! TTGTATTTGGTTTTCATAAT+AGG - chr1_4:60890325-60890344 Msa0175060:CDS 20.0%
!!! ATTCAATGATAATGTTTCAA+AGG - chr1_4:60890378-60890397 Msa0175060:CDS 20.0%
!! AGCCTAATTAAATAAAATGT+GGG - chr1_4:60890907-60890926 Msa0175060:five_prime_UTR 20.0%
!! TTGATTTTCAACAACTTATG+GGG + chr1_4:60885917-60885936 None:intergenic 25.0%
!!! GTTGATTTTCAACAACTTAT+GGG + chr1_4:60885918-60885937 None:intergenic 25.0%
!!! AGTTGATTTTCAACAACTTA+TGG + chr1_4:60885919-60885938 None:intergenic 25.0%
! AGAGATTATAGGAAAAAGAA+AGG + chr1_4:60885988-60886007 None:intergenic 25.0%
!! AAGATGAGGTATTTAATTGT+GGG + chr1_4:60886121-60886140 None:intergenic 25.0%
!! AAAGATGAGGTATTTAATTG+TGG + chr1_4:60886122-60886141 None:intergenic 25.0%
!! AAAGAGTACAGTTACATAAT+TGG + chr1_4:60886214-60886233 None:intergenic 25.0%
! GCATTTAGAAAATTATGTAG+AGG - chr1_4:60886286-60886305 Msa0175060:intron 25.0%
!! ATCTCTCTCTTTATGATTTT+TGG - chr1_4:60886610-60886629 Msa0175060:CDS 25.0%
!!! ACCAAATGGAATTAATTTGT+GGG + chr1_4:60886688-60886707 None:intergenic 25.0%
!! AACCAAATGGAATTAATTTG+TGG + chr1_4:60886689-60886708 None:intergenic 25.0%
!!! TTTTTTTTGAAGGAATTTGC+TGG + chr1_4:60887172-60887191 None:intergenic 25.0%
! CTATGTCAAAATACATTTGA+AGG + chr1_4:60887569-60887588 None:intergenic 25.0%
!!! CAAATGTATTTTGACATAGA+TGG - chr1_4:60887570-60887589 Msa0175060:intron 25.0%
! TATACTACGAGTTAAGTAAA+TGG + chr1_4:60887960-60887979 None:intergenic 25.0%
!!! AAGGAGGTATATATTTTACT+AGG - chr1_4:60888022-60888041 Msa0175060:intron 25.0%
!!! AGGAGGTATATATTTTACTA+GGG - chr1_4:60888023-60888042 Msa0175060:intron 25.0%
!! TATAGGAAGTTTCTTCTTTT+AGG - chr1_4:60888164-60888183 Msa0175060:intron 25.0%
!!! TATTTTTTTTGAGAGGAACT+TGG - chr1_4:60888670-60888689 Msa0175060:intron 25.0%
!!! ATTTTTTTTGAGAGGAACTT+GGG - chr1_4:60888671-60888690 Msa0175060:intron 25.0%
!!! GGTTCGACTTTTTTTTTTTA+AGG - chr1_4:60888692-60888711 Msa0175060:intron 25.0%
!!! TTTTTTTAAGGGAATGAACT+TGG - chr1_4:60888704-60888723 Msa0175060:intron 25.0%
!!! TTTTTTAAGGGAATGAACTT+GGG - chr1_4:60888705-60888724 Msa0175060:intron 25.0%
!! AAACAGTCTCAAAGTATTTA+TGG + chr1_4:60889096-60889115 None:intergenic 25.0%
!!! TTTTTGGAAAAATCCACATT+TGG + chr1_4:60889401-60889420 None:intergenic 25.0%
!! ATGTTCCAAATGTAGATTTT+TGG + chr1_4:60889417-60889436 None:intergenic 25.0%
!!! ACTTTTCCTTTTTCATTAAG+GGG + chr1_4:60889538-60889557 None:intergenic 25.0%
!!! TTGTATTTGTTGTTCACTAT+TGG + chr1_4:60889655-60889674 None:intergenic 25.0%
! ATAGTGAACAACAAATACAA+TGG - chr1_4:60889655-60889674 Msa0175060:intron 25.0%
!!! GTTAAGGTTGTTTTAATTCA+TGG - chr1_4:60890178-60890197 Msa0175060:intron 25.0%
!!! TATTTTTGTATATGCAATGC+AGG - chr1_4:60890205-60890224 Msa0175060:intron 25.0%
! TAACATCAATTCATGAGAAA+AGG + chr1_4:60890300-60890319 None:intergenic 25.0%
! TGTCAAATGTTATATTCAGT+TGG - chr1_4:60890354-60890373 Msa0175060:CDS 25.0%
! ATCATGAAAAACTATTCCTA+AGG - chr1_4:60890406-60890425 Msa0175060:CDS 25.0%
!! AATTTTGTCTGAATAATGAC+AGG - chr1_4:60890474-60890493 Msa0175060:CDS 25.0%
! GAGCCTAATTAAATAAAATG+TGG - chr1_4:60890906-60890925 Msa0175060:five_prime_UTR 25.0%
! GCCTAATTAAATAAAATGTG+GGG - chr1_4:60890908-60890927 Msa0175060:five_prime_UTR 25.0%
!!! TCCCCACATTTTATTTAATT+AGG + chr1_4:60890912-60890931 None:intergenic 25.0%
!! TTTGGAAAGTGCTATGAATA+TGG + chr1_4:60885827-60885846 None:intergenic 30.0%
AAAAGAAAGGCAAAAAAACG+TGG + chr1_4:60885975-60885994 None:intergenic 30.0%
TAAGAGAAAACTTGTGATGA+AGG + chr1_4:60886034-60886053 None:intergenic 30.0%
! TATGTAGTTGTTGTTAAGGT+TGG + chr1_4:60886064-60886083 None:intergenic 30.0%
! TGGATATGTAGTTGTTGTTA+AGG + chr1_4:60886068-60886087 None:intergenic 30.0%
TAGATTTAGCTAGAAAGATG+AGG + chr1_4:60886135-60886154 None:intergenic 30.0%
TAAGGTATGGAAAGAAAGAA+GGG + chr1_4:60886169-60886188 None:intergenic 30.0%
AGTACAGTTACATAATTGGA+TGG + chr1_4:60886210-60886229 None:intergenic 30.0%
!! AAATGGAATTAATTTGTGGG+CGG + chr1_4:60886685-60886704 None:intergenic 30.0%
GTCAGATTTAACTGTTTCAT+GGG - chr1_4:60886722-60886741 Msa0175060:intron 30.0%
TAAATAGTATTGTTTGCACG+TGG - chr1_4:60887010-60887029 Msa0175060:CDS 30.0%
!!! TTTTGACATAGATGGGTTAT+TGG - chr1_4:60887578-60887597 Msa0175060:intron 30.0%
AAAACAGTGCAAAAACTTGT+AGG - chr1_4:60887661-60887680 Msa0175060:intron 30.0%
CTAGTAACATGGGTTTATTA+GGG + chr1_4:60887756-60887775 None:intergenic 30.0%
ACTAGTAACATGGGTTTATT+AGG + chr1_4:60887757-60887776 None:intergenic 30.0%
ACAAATGCAACTAGTAACAT+GGG + chr1_4:60887766-60887785 None:intergenic 30.0%
AACAAATGCAACTAGTAACA+TGG + chr1_4:60887767-60887786 None:intergenic 30.0%
TATATGACAGCCAAAATTTC+AGG - chr1_4:60887976-60887995 Msa0175060:intron 30.0%
ATATGACAGCCAAAATTTCA+GGG - chr1_4:60887977-60887996 Msa0175060:intron 30.0%
! GACATGTTTTCTCAATATCA+AGG - chr1_4:60888003-60888022 Msa0175060:intron 30.0%
! ATGTTTTCTCAATATCAAGG+AGG - chr1_4:60888006-60888025 Msa0175060:intron 30.0%
! TAACACTACCCATTTTACTA+AGG - chr1_4:60888217-60888236 Msa0175060:intron 30.0%
! AACACTACCCATTTTACTAA+GGG - chr1_4:60888218-60888237 Msa0175060:intron 30.0%
! ATATTTTCTTAGGGTGTGTT+TGG + chr1_4:60888471-60888490 None:intergenic 30.0%
TTAGGTTAACAAGTGTCATA+AGG - chr1_4:60888800-60888819 Msa0175060:intron 30.0%
TAGGTTAACAAGTGTCATAA+GGG - chr1_4:60888801-60888820 Msa0175060:intron 30.0%
TTCAAAAGTTCTCCAAACAA+AGG - chr1_4:60889205-60889224 Msa0175060:intron 30.0%
GGATTAGAATAATGTGACAA+TGG + chr1_4:60889516-60889535 None:intergenic 30.0%
!! CTTTTCCTTTTTCATTAAGG+GGG + chr1_4:60889537-60889556 None:intergenic 30.0%
TGTTAAAATGTGTCACAGAT+TGG - chr1_4:60889580-60889599 Msa0175060:intron 30.0%
AATGGAGTAGAAACTCTTAT+GGG - chr1_4:60889673-60889692 Msa0175060:intron 30.0%
TTAGAGAACTTGAAATTTCG+CGG + chr1_4:60890108-60890127 None:intergenic 30.0%
GTCATGTCATTAAAAACCTT+AGG + chr1_4:60890425-60890444 None:intergenic 30.0%
TAAGATTGTGAGTAATGTTG+TGG - chr1_4:60890607-60890626 Msa0175060:five_prime_UTR 30.0%
!! AAAGCTTTGTTTGGTTAATG+TGG - chr1_4:60890996-60891015 Msa0175060:five_prime_UTR 30.0%
! GGTTAATGTGGATTTTCTAA+TGG - chr1_4:60891008-60891027 Msa0175060:five_prime_UTR 30.0%
ACCATTCATAATACAAACCA+TGG + chr1_4:60891045-60891064 None:intergenic 30.0%
TTCGAAGTTGGAGAGATTAT+AGG + chr1_4:60885999-60886018 None:intergenic 35.0%
AATCTCTCCAACTTCGAAAT+TGG - chr1_4:60886001-60886020 Msa0175060:three_prime_UTR 35.0%
TTGTGATCCAATTTCGAAGT+TGG + chr1_4:60886011-60886030 None:intergenic 35.0%
! AGTTGTTGTTAAGGTTGGAA+TGG + chr1_4:60886059-60886078 None:intergenic 35.0%
TCTAGCTAAATCTACAAACC+TGG - chr1_4:60886141-60886160 Msa0175060:CDS 35.0%
CTAGCTAAATCTACAAACCT+GGG - chr1_4:60886142-60886161 Msa0175060:CDS 35.0%
CTAAGGTATGGAAAGAAAGA+AGG + chr1_4:60886170-60886189 None:intergenic 35.0%
!!! GATTTTTGGTCAACGAGTTT+TGG - chr1_4:60886624-60886643 Msa0175060:CDS 35.0%
GCCCACAAATTAATTCCATT+TGG - chr1_4:60886684-60886703 Msa0175060:intron 35.0%
GGTCAGATTTAACTGTTTCA+TGG - chr1_4:60886721-60886740 Msa0175060:intron 35.0%
TGTAGGATATTAAGCTACAC+TGG - chr1_4:60887678-60887697 Msa0175060:intron 35.0%
! TTAAGCTACACTGGTTATCT+TGG - chr1_4:60887687-60887706 Msa0175060:intron 35.0%
TAAACGTGAACGAGTTGTTA+AGG - chr1_4:60887855-60887874 Msa0175060:intron 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG + chr1_4:60888064-60888083 None:intergenic 35.0%
!! AATCATTAACTTGTGCCTTG+AGG + chr1_4:60888065-60888084 None:intergenic 35.0%
TTTCTTAACATGTGCCCTAA+GGG - chr1_4:60888187-60888206 Msa0175060:intron 35.0%
AGTTTAGGAGGGAAAATGAA+GGG + chr1_4:60888442-60888461 None:intergenic 35.0%
TTTGGATTGAGAGTTTAGGA+GGG + chr1_4:60888453-60888472 None:intergenic 35.0%
TGTGTTTGGATTGAGAGTTT+AGG + chr1_4:60888457-60888476 None:intergenic 35.0%
! AAGTTGATTAAATGAGGGGT+AGG - chr1_4:60888528-60888547 Msa0175060:intron 35.0%
ATCGTGTCAATCTGAAAACT+TGG - chr1_4:60888638-60888657 Msa0175060:intron 35.0%
TCGTGTCAATCTGAAAACTT+GGG - chr1_4:60888639-60888658 Msa0175060:intron 35.0%
TCAATCTGAAAACTTGGGTT+AGG - chr1_4:60888644-60888663 Msa0175060:intron 35.0%
!! TGTCATAAGGGCATTGTTTA+AGG - chr1_4:60888813-60888832 Msa0175060:intron 35.0%
CTACATGGTTAACCTTTGTT+TGG + chr1_4:60889220-60889239 None:intergenic 35.0%
AGCATTGCTTGAGTTATTAG+TGG - chr1_4:60889320-60889339 Msa0175060:intron 35.0%
CACATAGAATGTTCCAAATG+TGG - chr1_4:60889385-60889404 Msa0175060:intron 35.0%
!! AGAGCTACTTTGGAGTTTAT+TGG + chr1_4:60889441-60889460 None:intergenic 35.0%
GTAGAATGAAAGAGCTACTT+TGG + chr1_4:60889451-60889470 None:intergenic 35.0%
CTAATCCCCCTTAATGAAAA+AGG - chr1_4:60889529-60889548 Msa0175060:intron 35.0%
CAATGGAGTAGAAACTCTTA+TGG - chr1_4:60889672-60889691 Msa0175060:intron 35.0%
CTATCAATTGGTCATATCTC+AGG - chr1_4:60889736-60889755 Msa0175060:intron 35.0%
TTTGTATATGCAATGCAGGT+TGG - chr1_4:60890209-60890228 Msa0175060:intron 35.0%
TTGCTCAACATTCTTATCTC+AGG - chr1_4:60890266-60890285 Msa0175060:intron 35.0%
TGCTCAACATTCTTATCTCA+GGG - chr1_4:60890267-60890286 Msa0175060:intron 35.0%
AACATTCTTATCTCAGGGTA+TGG - chr1_4:60890272-60890291 Msa0175060:intron 35.0%
ATATTCGGAGACTAACTTGA+TGG + chr1_4:60890453-60890472 None:intergenic 35.0%
ATGTTGTGGTTGTAAATGCT+AGG - chr1_4:60890621-60890640 Msa0175060:five_prime_UTR 35.0%
CAAGCATCTCTGGATATATT+TGG - chr1_4:60890709-60890728 Msa0175060:five_prime_UTR 35.0%
GTTATCACGAAGCTTGATAA+GGG + chr1_4:60890777-60890796 None:intergenic 35.0%
CTTATCAAGCTTCGTGATAA+CGG - chr1_4:60890776-60890795 Msa0175060:five_prime_UTR 35.0%
AGCTTGAAATATGAGAAGTG+GGG + chr1_4:60890869-60890888 None:intergenic 35.0%
GAGCTTGAAATATGAGAAGT+GGG + chr1_4:60890870-60890889 None:intergenic 35.0%
AGAGCTTGAAATATGAGAAG+TGG + chr1_4:60890871-60890890 None:intergenic 35.0%
!! TGTGTCATGAAAGCTTTGTT+TGG - chr1_4:60890987-60891006 Msa0175060:five_prime_UTR 35.0%
! GCCATGGTTTGTATTATGAA+TGG - chr1_4:60891041-60891060 Msa0175060:five_prime_UTR 35.0%
TCAACAACTTATGGGGATGA+TGG + chr1_4:60885910-60885929 None:intergenic 40.0%
CAAAAAAACGTGGTTGAAGG+TGG + chr1_4:60885965-60885984 None:intergenic 40.0%
AGGCAAAAAAACGTGGTTGA+AGG + chr1_4:60885968-60885987 None:intergenic 40.0%
TCAACGTGAGTCATTTGCAA+TGG - chr1_4:60886484-60886503 Msa0175060:intron 40.0%
CAACGTGAGTCATTTGCAAT+GGG - chr1_4:60886485-60886504 Msa0175060:intron 40.0%
AACGTGAGTCATTTGCAATG+GGG - chr1_4:60886486-60886505 Msa0175060:intron 40.0%
!! CAACTATTGCTTTTACCACC+CGG + chr1_4:60886530-60886549 None:intergenic 40.0%
! TTCTGAGAAAGAGAGACACT+TGG + chr1_4:60886584-60886603 None:intergenic 40.0%
AAGTGTCTCTCTTTCTCAGA+AGG - chr1_4:60886583-60886602 Msa0175060:intron 40.0%
CATGCAAGCTAGAAACCAAA+TGG + chr1_4:60886702-60886721 None:intergenic 40.0%
!! CATTTGGTTTCTAGCTTGCA+TGG - chr1_4:60886700-60886719 Msa0175060:intron 40.0%
TGTGATGAGATGTGCATGAT+TGG - chr1_4:60886967-60886986 Msa0175060:CDS 40.0%
GTGATGAGATGTGCATGATT+GGG - chr1_4:60886968-60886987 Msa0175060:CDS 40.0%
GTTTCTTAACATGTGCCCTA+AGG - chr1_4:60888186-60888205 Msa0175060:intron 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG + chr1_4:60888204-60888223 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG + chr1_4:60888205-60888224 None:intergenic 40.0%
CCACACACCCTTAGTAAAAT+GGG + chr1_4:60888228-60888247 None:intergenic 40.0%
TCCACACACCCTTAGTAAAA+TGG + chr1_4:60888229-60888248 None:intergenic 40.0%
!! TTTTACTAAGGGTGTGTGGA+TGG - chr1_4:60888229-60888248 Msa0175060:intron 40.0%
ATGGAGAGTTTAAGAGAGGA+TGG - chr1_4:60888248-60888267 Msa0175060:intron 40.0%
TGGAGAGTTTAAGAGAGGAT+GGG - chr1_4:60888249-60888268 Msa0175060:intron 40.0%
GTTTAGGAGGGAAAATGAAG+GGG + chr1_4:60888441-60888460 None:intergenic 40.0%
GAGTTTAGGAGGGAAAATGA+AGG + chr1_4:60888443-60888462 None:intergenic 40.0%
GTTTGGATTGAGAGTTTAGG+AGG + chr1_4:60888454-60888473 None:intergenic 40.0%
CTAATATATGCAGCAACAGC+AGG - chr1_4:60889628-60889647 Msa0175060:intron 40.0%
AGAGACTTGTGCTACAATGT+AGG + chr1_4:60889833-60889852 None:intergenic 40.0%
AATGAATTGAACGTACCGCA+CGG + chr1_4:60890014-60890033 None:intergenic 40.0%
!! TTTCGCGGCAACTTTTTAAG+TGG + chr1_4:60890093-60890112 None:intergenic 40.0%
GTCTGAATAATGACAGGATC+AGG - chr1_4:60890480-60890499 Msa0175060:CDS 40.0%
CTTGTAATTTCCTGCTGCAA+CGG + chr1_4:60890693-60890712 None:intergenic 40.0%
CAGGAAATTACAAGCATCTC+TGG - chr1_4:60890699-60890718 Msa0175060:five_prime_UTR 40.0%
GCATCTCTGGATATATTTGG+TGG - chr1_4:60890712-60890731 Msa0175060:five_prime_UTR 40.0%
CGTTATCACGAAGCTTGATA+AGG + chr1_4:60890778-60890797 None:intergenic 40.0%
GCTTGAAATATGAGAAGTGG+GGG + chr1_4:60890868-60890887 None:intergenic 40.0%
TTCACACACACTCGATTACA+AGG + chr1_4:60890937-60890956 None:intergenic 40.0%
TAATGGCAAGATTCTAGCCA+TGG - chr1_4:60891025-60891044 Msa0175060:five_prime_UTR 40.0%
! ATGATGGAGACGAAGTGATG+AGG + chr1_4:60885894-60885913 None:intergenic 45.0%
TGGATGAGAGGACTAATGGT+CGG + chr1_4:60885945-60885964 None:intergenic 45.0%
AAGGTGGATGAGAGGACTAA+TGG + chr1_4:60885949-60885968 None:intergenic 45.0%
AAAATGCCAGAATCGGAGTC+AGG - chr1_4:60886377-60886396 Msa0175060:intron 45.0%
AAATGCCAGAATCGGAGTCA+GGG - chr1_4:60886378-60886397 Msa0175060:intron 45.0%
AGATGAGAATAATGGCCCTC+CGG + chr1_4:60886444-60886463 None:intergenic 45.0%
TGCATCGCTAATTTCTCTGC+TGG - chr1_4:60886548-60886567 Msa0175060:intron 45.0%
!! TGGAATTAATTTGTGGGCGG+TGG + chr1_4:60886682-60886701 None:intergenic 45.0%
GACAGCCAAAATTTCAGGGA+CGG - chr1_4:60887981-60888000 Msa0175060:intron 45.0%
! CATGTCCGTCCCTGAAATTT+TGG + chr1_4:60887989-60888008 None:intergenic 45.0%
!! AACGTTAACTTGTGCCCTCA+AGG - chr1_4:60888047-60888066 Msa0175060:intron 45.0%
!! CCCATTTTACTAAGGGTGTG+TGG - chr1_4:60888225-60888244 Msa0175060:intron 45.0%
GTGGATGGAGAGTTTAAGAG+AGG - chr1_4:60888244-60888263 Msa0175060:intron 45.0%
AGAGTTTAAGAGAGGATGGG+AGG - chr1_4:60888252-60888271 Msa0175060:intron 45.0%
GAGTTTAAGAGAGGATGGGA+GGG - chr1_4:60888253-60888272 Msa0175060:intron 45.0%
AGTTTAAGAGAGGATGGGAG+GGG - chr1_4:60888254-60888273 Msa0175060:intron 45.0%
ACAGATTGGAGCAGAGTTTG+TGG - chr1_4:60889594-60889613 Msa0175060:intron 45.0%
CAGATTGGAGCAGAGTTTGT+GGG - chr1_4:60889595-60889614 Msa0175060:intron 45.0%
ACTTGTGCTACAATGTAGGC+TGG + chr1_4:60889829-60889848 None:intergenic 45.0%
GTTTACCATCCTTTCCTCTC+TGG - chr1_4:60889886-60889905 Msa0175060:intron 45.0%
CTTGTCCAACGCTAACAGTA+GGG + chr1_4:60889924-60889943 None:intergenic 45.0%
ATGAATTGAACGTACCGCAC+GGG + chr1_4:60890013-60890032 None:intergenic 45.0%
!! TGCAATGCAGGTTGGTGAAT+TGG - chr1_4:60890217-60890236 Msa0175060:intron 45.0%
GAATTGGCTGGTATTGCTGT+TGG - chr1_4:60890233-60890252 Msa0175060:intron 45.0%
GTAAATGCTAGGCCAACAAG+CGG - chr1_4:60890632-60890651 Msa0175060:five_prime_UTR 45.0%
!! AAAATTAAAAAAAAAAAGTT+AGG - chr1_4:60888782-60888801 Msa0175060:intron 5.0%
!!! TTTATTTTACATTTATTTTT+AGG + chr1_4:60888935-60888954 None:intergenic 5.0%
TGGAAAGAAAGAAGGGACCC+AGG + chr1_4:60886162-60886181 None:intergenic 50.0%
GATGAGAATAATGGCCCTCC+GGG + chr1_4:60886443-60886462 None:intergenic 50.0%
GTGAGTCATTTGCAATGGGG+AGG - chr1_4:60886489-60886508 Msa0175060:intron 50.0%
GAGGTGCAACATGTGTATGC+CGG - chr1_4:60886508-60886527 Msa0175060:intron 50.0%
AGGTGCAACATGTGTATGCC+GGG - chr1_4:60886509-60886528 Msa0175060:intron 50.0%
ATGGGAAATGCAGCTTGTGC+TGG - chr1_4:60889691-60889710 Msa0175060:intron 50.0%
ATCACTCACCATTGCCTTCG+CGG - chr1_4:60889774-60889793 Msa0175060:intron 50.0%
!! TGCGCTTGTTATGCGCTCAA+AGG - chr1_4:60889862-60889881 Msa0175060:intron 50.0%
TACCATCCTTTCCTCTCTGG+TGG - chr1_4:60889889-60889908 Msa0175060:intron 50.0%
ACTGTCCCTACTGTTAGCGT+TGG - chr1_4:60889916-60889935 Msa0175060:intron 50.0%
GCTTGTCCAACGCTAACAGT+AGG + chr1_4:60889925-60889944 None:intergenic 50.0%
!! TGTTGCTACTGATTCCCGTG+CGG - chr1_4:60889996-60890015 Msa0175060:intron 50.0%
!! ATGCAGGTTGGTGAATTGGC+TGG - chr1_4:60890221-60890240 Msa0175060:intron 50.0%
TTCATGGAACCACCGCTTGT+TGG + chr1_4:60890647-60890666 None:intergenic 50.0%
AAGCGGTGGTTCCATGAATC+CGG - chr1_4:60890649-60890668 Msa0175060:five_prime_UTR 50.0%
TAATTTCCTGCTGCAACGGC+TGG + chr1_4:60890689-60890708 None:intergenic 50.0%
CGTGGTTGAAGGTGGATGAG+AGG + chr1_4:60885957-60885976 None:intergenic 55.0%
TGCAACATGTGTATGCCGGG+TGG - chr1_4:60886512-60886531 Msa0175060:intron 55.0%
GCGAAGGCAATGGTGAGTGA+TGG + chr1_4:60889775-60889794 None:intergenic 55.0%
GAACATGTGCCCACGGGAAA+TGG + chr1_4:60889807-60889826 None:intergenic 55.0%
TAGGCTGGAACATGTGCCCA+CGG + chr1_4:60889814-60889833 None:intergenic 55.0%
CACCACCAGAGAGGAAAGGA+TGG + chr1_4:60889894-60889913 None:intergenic 55.0%
GTGACACCACCAGAGAGGAA+AGG + chr1_4:60889898-60889917 None:intergenic 55.0%
AATGCTAGGCCAACAAGCGG+TGG - chr1_4:60890635-60890654 Msa0175060:five_prime_UTR 55.0%
TAAGGTGCGCACCGGATTCA+TGG + chr1_4:60890663-60890682 None:intergenic 55.0%
ATGAATCCGGTGCGCACCTT+AGG - chr1_4:60890662-60890681 Msa0175060:five_prime_UTR 55.0%
!! TATTTGGTGGCACCGACGCT+TGG - chr1_4:60890725-60890744 Msa0175060:five_prime_UTR 55.0%
!! CTTGGTGCTCTAGCTGGTTC+TGG - chr1_4:60890743-60890762 Msa0175060:five_prime_UTR 55.0%
CTGAACCACCTCAACCGGTT+AGG - chr1_4:60890801-60890820 Msa0175060:five_prime_UTR 55.0%
AAGCTCCTAACCGGTTGAGG+TGG + chr1_4:60890809-60890828 None:intergenic 55.0%
TAGCGACGGAAGCTCCTAAC+CGG + chr1_4:60890818-60890837 None:intergenic 55.0%
GGTGTCCCTGACTCCGATTC+TGG + chr1_4:60886386-60886405 None:intergenic 60.0%
ATCGGAGTCAGGGACACCGA+CGG - chr1_4:60886388-60886407 Msa0175060:intron 60.0%
!! AATGGCCCTCCGGGAGTATC+CGG + chr1_4:60886434-60886453 None:intergenic 60.0%
GAAATGGCGAAACGCCGCGA+AGG + chr1_4:60889791-60889810 None:intergenic 60.0%
GGCGTTTCGCCATTTCCCGT+GGG - chr1_4:60889795-60889814 Msa0175060:intron 60.0%
AGGCTGGAACATGTGCCCAC+GGG + chr1_4:60889813-60889832 None:intergenic 60.0%
GGACAGTGACACCACCAGAG+AGG + chr1_4:60889903-60889922 None:intergenic 60.0%
TGCTGCAACGGCTGGACCTA+AGG + chr1_4:60890681-60890700 None:intergenic 60.0%
TTAGGTCCAGCCGTTGCAGC+AGG - chr1_4:60890680-60890699 Msa0175060:five_prime_UTR 60.0%
! CCAGCTAGAGCACCAAGCGT+CGG + chr1_4:60890740-60890759 None:intergenic 60.0%
CGGAGCTGAACCACCTCAAC+CGG - chr1_4:60890796-60890815 Msa0175060:five_prime_UTR 60.0%
CGGAAGCTCCTAACCGGTTG+AGG + chr1_4:60890812-60890831 None:intergenic 60.0%
!! AAGTGGGGGTGTCGAGAGCA+GGG + chr1_4:60890854-60890873 None:intergenic 60.0%
CGACACCGGATACTCCCGGA+GGG - chr1_4:60886426-60886445 Msa0175060:intron 65.0%
GCGAAACGCCGCGAAGGCAA+TGG + chr1_4:60889785-60889804 None:intergenic 65.0%
CGGCGTTTCGCCATTTCCCG+TGG - chr1_4:60889794-60889813 Msa0175060:intron 65.0%
GCTGGACCTAAGGTGCGCAC+CGG + chr1_4:60890671-60890690 None:intergenic 65.0%
!! CCGACGCTTGGTGCTCTAGC+TGG - chr1_4:60890737-60890756 Msa0175060:five_prime_UTR 65.0%
GGAGCTTCCGTCGCTAGAGC+TGG - chr1_4:60890822-60890841 Msa0175060:five_prime_UTR 65.0%
! GTCGCAGCCAGCTCTAGCGA+CGG + chr1_4:60890832-60890851 None:intergenic 65.0%
!! GAAGTGGGGGTGTCGAGAGC+AGG + chr1_4:60890855-60890874 None:intergenic 65.0%
AGGGACACCGACGGCAGCGT+CGG - chr1_4:60886397-60886416 Msa0175060:intron 70.0%
!! CCGGGAGTATCCGGTGTCGC+CGG + chr1_4:60886425-60886444 None:intergenic 70.0%
CCGGCGACACCGGATACTCC+CGG - chr1_4:60886422-60886441 Msa0175060:intron 70.0%
GCGACACCGGATACTCCCGG+AGG - chr1_4:60886425-60886444 Msa0175060:intron 70.0%
!! ACCGACGGCAGCGTCGGTGC+CGG - chr1_4:60886403-60886422 Msa0175060:intron 75.0%
!! AGCGTCGGTGCCGGCGACAC+CGG - chr1_4:60886412-60886431 Msa0175060:intron 75.0%
!!! GCCGGCACCGACGCTGCCGT+CGG + chr1_4:60886407-60886426 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 60885791 60891099 60885791 ID=Msa0175060;Name=Msa0175060
chr1_4 mRNA 60885791 60891099 60885791 ID=Msa0175060-mRNA-1;Parent=Msa0175060;Name=Msa0175060-mRNA-1;_AED=0.03;_eAED=0.03;_QI=589|1|1|1|1|1|4|261|300
chr1_4 exon 60885791 60886247 60885791 ID=Msa0175060-mRNA-1:exon:19417;Parent=Msa0175060-mRNA-1
chr1_4 exon 60886602 60886663 60886602 ID=Msa0175060-mRNA-1:exon:19416;Parent=Msa0175060-mRNA-1
chr1_4 exon 60886873 60887292 60886873 ID=Msa0175060-mRNA-1:exon:19415;Parent=Msa0175060-mRNA-1
chr1_4 exon 60890286 60891099 60890286 ID=Msa0175060-mRNA-1:exon:19414;Parent=Msa0175060-mRNA-1
chr1_4 five_prime_UTR 60890511 60891099 60890511 ID=Msa0175060-mRNA-1:five_prime_utr;Parent=Msa0175060-mRNA-1
chr1_4 CDS 60890286 60890510 60890286 ID=Msa0175060-mRNA-1:cds;Parent=Msa0175060-mRNA-1
chr1_4 CDS 60886873 60887292 60886873 ID=Msa0175060-mRNA-1:cds;Parent=Msa0175060-mRNA-1
chr1_4 CDS 60886602 60886663 60886602 ID=Msa0175060-mRNA-1:cds;Parent=Msa0175060-mRNA-1
chr1_4 CDS 60886052 60886247 60886052 ID=Msa0175060-mRNA-1:cds;Parent=Msa0175060-mRNA-1
chr1_4 three_prime_UTR 60885791 60886051 60885791 ID=Msa0175060-mRNA-1:three_prime_utr;Parent=Msa0175060-mRNA-1
Gene Sequence

>Msa0175060

ATGCCAGAATCGGAGTCAGGGACACCGACGGCAGCGTCGGTGCCGGCGACACCGGATACTCCCGGAGGGCCATTATTCTCATCTATAAGAATTGATTCACTTGATCAACGTGAGTCATTTGCAATGGGGAGGTGCAACATGTGTATGCCGGGTGGTAAAAGCAATAGTTGCATCGCTAATTTCTCTGCTGGAGTTCGTATTCCAAGTGTCTCTCTTTCTCAGAAGATTGGAGCAGAGTTTGTGGGAACATTCATACTAATATATGCAGCAACAGCAGGACCAATAGTGAACAACAAATACAATGGAGTAGAAACTCTTATGGGAAATGCAGCTTGTGCTGGACTAACAGTCATGTTCATTATTCTATCAATTGGTCATATCTCAGGTGCACATCTCAATCCATCACTCACCATTGCCTTCGCGGCGTTTCGCCATTTCCCGTGGGCACATGTTCCAGCCTACATTGTAGCACAAGTCTCTGCTTCGATTTGCGCTTGTTATGCGCTCAAAGGAGTTTACCATCCTTTCCTCTCTGGTGGTGTCACTGTCCCTACTGTTAGCGTTGGACAAGCTTTTGCAACTGAGTTTATCATCACTTTTATTCTCTTGTTTGTTGTTACTGCTGTTGCTACTGATTCCCGTGCGGTTGGTGAATTGGCTGGTATTGCTGTTGGTGCTACAGTTTTGCTCAACATTCTTATCTCAGGGCCAACAAGCGGTGGTTCCATGAATCCGGTGCGCACCTTAGGTCCAGCCGTTGCAGCAGGAAATTACAAGCATCTCTGGATATATTTGGTGGCACCGACGCTTGGTGCTCTAGCTGGTTCTGGAGTTTATACCCTTATCAAGCTTCGTGATAACGGAGCTGAACCACCTCAACCGGTTAGGAGCTTCCGTCGCTAG
Protein sequence

>Msa0175060

MPESESGTPTAASVPATPDTPGGPLFSSIRIDSLDQRESFAMGRCNMCMPGGKSNSCIANFSAGVRIPSVSLSQKIGAEFVGTFILIYAATAGPIVNNKYNGVETLMGNAACAGLTVMFIILSIGHISGAHLNPSLTIAFAAFRHFPWAHVPAYIVAQVSASICACYALKGVYHPFLSGGVTVPTVSVGQAFATEFIITFILLFVVTAVATDSRAVGELAGIAVGATVLLNILISGPTSGGSMNPVRTLGPAVAAGNYKHLWIYLVAPTLGALAGSGVYTLIKLRDNGAEPPQPVRSFRR*