AlfalfaGEDB | Msa0096860

M. sativa cultivar zhongmu-4 / Msa0096860

KEGG: -
GO: -
PFAM: PF03479.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096860	sp\|Q8GWQ2\|AHL20_ARATH	61.486	296	92	7	1	289	1	281	2.86e-103	305

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096860	A0A072VM41	97.241	290	7	1	1	289	33	322	0.0	562

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa0096860	0.811241	7.726884e-51	-8.615850e-47
Msa0011510	Msa0096860	0.802811	4.698687e-49	-8.615850e-47
Msa0021870	Msa0096860	0.812440	4.234521e-51	-8.615850e-47
Msa0027860	Msa0096860	0.806878	6.642382e-50	-8.615850e-47
Msa0031650	Msa0096860	0.826144	3.187332e-54	-8.615850e-47
Msa0038010	Msa0096860	0.841951	3.479447e-58	-8.615850e-47
Msa0046390	Msa0096860	0.804186	2.437554e-49	-8.615850e-47
Msa0048950	Msa0096860	0.810708	1.007615e-50	-8.615850e-47
Msa0054760	Msa0096860	-0.802854	4.603197e-49	-8.615850e-47
Msa0059880	Msa0096860	0.819474	1.139651e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096860	MtrunA17_Chr1g0186441	77.241	290	19	2	1	289	4	247	3.70e-118	339
Msa0096860	MtrunA17_Chr1g0168121	68.345	278	72	5	25	289	1	275	5.72e-103	301
Msa0096860	MtrunA17_Chr1g0190051	54.313	313	111	10	1	289	1	305	1.17e-79	243
Msa0096860	MtrunA17_Chr3g0112131	59.910	222	71	5	69	278	86	301	1.68e-68	215
Msa0096860	MtrunA17_Chr5g0443311	56.364	220	76	5	62	281	51	250	2.19e-65	205
Msa0096860	MtrunA17_Chr5g0398531	74.026	154	40	0	62	215	100	253	8.74e-65	206
Msa0096860	MtrunA17_Chr8g0351651	60.262	229	84	6	62	289	86	308	1.19e-63	202
Msa0096860	MtrunA17_Chr7g0250031	50.923	271	108	5	12	270	1	258	1.87e-63	201
Msa0096860	MtrunA17_Chr3g0133051	50.556	180	85	2	54	229	66	245	1.83e-58	188
Msa0096860	MtrunA17_Chr4g0054651	57.789	199	73	3	69	265	83	272	3.68e-58	188

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096860	AT4G14465.1	61.486	296	92	7	1	289	1	281	2.91e-104	305
Msa0096860	AT3G04570.1	62.762	239	59	5	1	211	1	237	4.31e-80	245
Msa0096860	AT1G76500.1	63.102	187	55	4	38	213	49	232	1.99e-63	202
Msa0096860	AT2G35270.1	65.161	155	54	0	62	216	78	232	3.76e-62	198
Msa0096860	AT2G45430.1	59.292	226	81	6	62	280	89	310	6.21e-62	199
Msa0096860	AT2G42940.1	64.286	154	55	0	62	215	53	206	3.68e-61	194
Msa0096860	AT4G22810.1	54.357	241	76	5	62	289	105	324	8.09e-61	196
Msa0096860	AT3G60870.1	55.838	197	76	4	19	206	7	201	4.39e-60	192
Msa0096860	AT4G12050.1	70.323	155	45	1	62	215	118	272	1.67e-57	188
Msa0096860	AT5G49700.1	41.304	276	119	9	25	289	33	276	1.89e-57	186

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Find 106 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCATGGAAGAAGGATC+TGG	0.482278	1_3:+2565615	None:intergenic
AACAGAGATGAACCTAGAGA+AGG	0.663205	1_3:-2566224	Msa0096860:CDS
AACTGATGACCAATTCCTGG+TGG	0.683488	1_3:+2565651	None:intergenic
AACTGGTCTTACAGTGTACT+TGG	0.444380	1_3:-2565871	Msa0096860:CDS
AATCCTTGGTGGACAATTCA+AGG	0.465438	1_3:-2566359	Msa0096860:CDS
AATGAAGGGCCTGGTTCTGC+GGG	0.484847	1_3:-2565711	Msa0096860:CDS
AATGATAATAATGAAGGGCC+TGG	0.537636	1_3:-2565720	Msa0096860:CDS
AATTCAAGGAGGGTTATCTG+GGG	0.712203	1_3:-2566345	Msa0096860:CDS
AATTTGTTGCCTAATAATGG+AGG	0.564310	1_3:-2565576	Msa0096860:CDS
ACAACAGCACCAGGAGCTGA+AGG	0.601528	1_3:+2565966	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATCTCTTGTTACAAAGAT+GGG	+	chr1_3:2565752-2565771	None:intergenic	30.0%
TTATTAGGCAACAAATTTGG+TGG	+	chr1_3:2566304-2566323	None:intergenic	30.0%
CCATTATTAGGCAACAAATT+TGG	+	chr1_3:2566307-2566326	None:intergenic	30.0%
CTATCTCTTGTTACAAAGAT+GGG	+	chr1_3:2565752-2565771	None:intergenic	30.0%
TTATTAGGCAACAAATTTGG+TGG	+	chr1_3:2566304-2566323	None:intergenic	30.0%
CCATTATTAGGCAACAAATT+TGG	+	chr1_3:2566307-2566326	None:intergenic	30.0%
TGGTGTTTGTTTAAACCACT+TGG	+	chr1_3:2565584-2565603	None:intergenic	35.0%
TGATTGTTAGGTTTGTGTTG+TGG	+	chr1_3:2565604-2565623	None:intergenic	35.0%
GCTATCTCTTGTTACAAAGA+TGG	+	chr1_3:2565753-2565772	None:intergenic	35.0%
AGTAGCATTAGTAAACGTAG+CGG	+	chr1_3:2566113-2566132	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	2565518	2566387	2565518	ID=Msa0096860;Name=Msa0096860
chr1_3	mRNA	2565518	2566387	2565518	ID=Msa0096860-mRNA-1;Parent=Msa0096860;Name=Msa0096860-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|289
chr1_3	exon	2565518	2566387	2565518	ID=Msa0096860-mRNA-1:exon:678;Parent=Msa0096860-mRNA-1
chr1_3	CDS	2565518	2566387	2565518	ID=Msa0096860-mRNA-1:cds;Parent=Msa0096860-mRNA-1

Gene Sequence

>Msa0096860

CTGGCTAATCCTTGGTGGACAATTCAAGGAGGGTTATCTGGGGTTGACCCAGGAACCCACTCACCAAGTGGTTTAAACAAACACCACAACACAAACCTAACAATCAATGAAAATCACGATGAAGAAGAAGACGTTGACGACAACAGAGATGAACCTAGAGAAGGTGCAGTTGAAGTTGGTAACCGAAGACCAAGAGGAAGACCACCTGGATCCAAAAACAGACCAAAACCACCCATCTTTGTAACAAGAGATAGCCCAAATGCTTTGAAGAGCCATGTCATGGAGGTAGCCGGTGGAGCTGATATAGCAGAAAGTGTGGCACAATTCGCAAGAAGGCGTCAACGCGGTGTTTGTGTTCTCAGTGGAAGTGGCTCTGTAGCTAATGTTACTCTTAGACAACCTTCAGCTCCTGGTGCTGTTGTAGCACTTCATGGTAGGTTTGAGATTTTGTCCTTAACCGGGACTTTTCTACCTGGTCCTGCTCCACCAGGTTCAACTGGTCTTACAGTGTACTTGGCTGGAGGTCAGGGTCAGGTAGTTGGAGGTTGTGTTGTTGGAACTCTTGTTGCAGTTGGATCTGTTATGCTTATTGCCGCTACGTTTACTAATGCTACTTATGAGAGACTTCCACTTGATGACGATGATAATGATAATAATGAAGGGCCTGGTTCTGCGGGAGGTGTGCAAGGTGGAGGAGCGTCTGGTGGATCGCCTCCACCAGGAATTGGTCATCAGTTGCAGGGTGGGCTTCCAGATCCTTCTTCCATGCCTTTGTATAATTTGCCACCAAATTTGTTGCCTAATAATGGAGGGCAGATGGGACATGAGGCTCTTGCCTGGGCTGTTGCTCATGGAAGACCACCTTATTGA

Protein sequence

>Msa0096860

LANPWWTIQGGLSGVDPGTHSPSGLNKHHNTNLTINENHDEEEDVDDNRDEPREGAVEVGNRRPRGRPPGSKNRPKPPIFVTRDSPNALKSHVMEVAGGADIAESVAQFARRRQRGVCVLSGSGSVANVTLRQPSAPGAVVALHGRFEILSLTGTFLPGPAPPGSTGLTVYLAGGQGQVVGGCVVGTLVAVGSVMLIAATFTNATYERLPLDDDDNDNNEGPGSAGGVQGGGASGGSPPPGIGHQLQGGLPDPSSMPLYNLPPNLLPNNGGQMGHEALAWAVAHGRPPY*