AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41636


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41636.t1 MTR_3g098520 96.226 159 5 1 1 158 1 159 3.90e-111 312
MS.gene41636.t1 MTR_3g098520 95.745 94 3 1 1 93 1 94 8.44e-61 184
MS.gene41636.t1 MTR_5g008050 50.000 60 24 2 55 110 181 238 2.76e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41636.t1 AT5G51010 59.748 159 56 4 1 157 1 153 2.39e-60 184
MS.gene41636.t1 AT5G17170 41.584 101 33 5 55 150 180 259 6.52e-13 64.7

Find 48 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCAATTGCTTCATCTCTT+TGG 0.237136 3.4:+88368836 None:intergenic
GTTGATTAGGATGAAGTAAA+AGG 0.290725 3.4:+88369758 None:intergenic
TAAGCAACTTGTAGTTGATT+AGG 0.313376 3.4:+88369745 None:intergenic
CCAGGGCAGAAGTATTTATC+AGG 0.317364 3.4:+88369531 None:intergenic
AATCAACTACAAGTTGCTTA+TGG 0.342983 3.4:-88369742 MS.gene41636:CDS
AAAGATGTCTTGAGAGTAGA+TGG 0.349045 3.4:+88369799 None:intergenic
TAGGATGAAGTAAAAGGTTA+AGG 0.356489 3.4:+88369764 None:intergenic
ACGACAGATATAAGCTTGTT+TGG 0.361476 3.4:+88369690 None:intergenic
GTGGGAGTTGTAGTTCTTGC+AGG 0.375454 3.4:-88368690 MS.gene41636:CDS
CTCCAAAGAGATGAAGCAAT+TGG 0.389726 3.4:-88368838 MS.gene41636:CDS
ACTCCCACGGCAACTGCAAT+AGG 0.395621 3.4:+88368705 None:intergenic
ATAGGGCTTAAACCTTCGTT+TGG 0.408207 3.4:+88368921 None:intergenic
AGCTACACGCATGGTAAACC+TGG 0.428236 3.4:+88369714 None:intergenic
TTTGTTGACATCTGTTGCAT+AGG 0.431914 3.4:+88368903 None:intergenic
TTGTTGACATCTGTTGCATA+GGG 0.437509 3.4:+88368904 None:intergenic
TTGGAGAGAGGTCGGAGGGT+AGG 0.447942 3.4:+88369823 None:intergenic
TGTCTTGAGAGTAGATGGAT+TGG 0.449903 3.4:+88369804 None:intergenic
TAGATGGATTGGAGAGAGGT+CGG 0.467506 3.4:+88369815 None:intergenic
AGGATGAAGTAAAAGGTTAA+GGG 0.468286 3.4:+88369765 None:intergenic
TAGGGCTTAAACCTTCGTTT+GGG 0.473259 3.4:+88368922 None:intergenic
TTATCAGGCAATTTATCAAA+GGG 0.481492 3.4:+88369546 None:intergenic
GTGATAATCTTGTAGTGTGA+AGG 0.484953 3.4:+88369857 None:intergenic
TCCAAAGAGATGAAGCAATT+GGG 0.489224 3.4:-88368837 MS.gene41636:intron
TGATAATCTTGTAGTGTGAA+GGG 0.498122 3.4:+88369858 None:intergenic
CTTATATCTGTCGTGACTGC+GGG 0.498593 3.4:-88369681 MS.gene41636:intron
TTTATCAGGCAATTTATCAA+AGG 0.502560 3.4:+88369545 None:intergenic
TGGATTGGAGAGAGGTCGGA+GGG 0.504235 3.4:+88369819 None:intergenic
CAAAATGGCCTTGCAAGTGC+AGG 0.518248 3.4:-88369884 None:intergenic
AAATGAAACAGATGTTAGAA+AGG 0.526185 3.4:-88368875 MS.gene41636:CDS
ACTTCCTATTGCAGTTGCCG+TGG 0.541016 3.4:-88368709 MS.gene41636:CDS
CCTGATAAATACTTCTGCCC+TGG 0.541318 3.4:-88369531 MS.gene41636:intron
CTTCCTATTGCAGTTGCCGT+GGG 0.547663 3.4:-88368708 MS.gene41636:CDS
ATGGATTGGAGAGAGGTCGG+AGG 0.556394 3.4:+88369818 None:intergenic
GAGAGGTCGGAGGGTAGGAG+TGG 0.557603 3.4:+88369828 None:intergenic
ATCAGGCAATTTATCAAAGG+GGG 0.560795 3.4:+88369548 None:intergenic
AAGGGAGGCCTGCACTTGCA+AGG 0.564120 3.4:+88369876 None:intergenic
GCAGGTATATTTACAATGAG+AGG 0.564968 3.4:-88369572 MS.gene41636:intron
GCTTATATCTGTCGTGACTG+CGG 0.577229 3.4:-88369682 MS.gene41636:CDS
CGGAGGGTAGGAGTGGCAGT+AGG 0.583426 3.4:+88369835 None:intergenic
AAGTTGCTTATGGACCTCCC+AGG 0.608931 3.4:-88369732 MS.gene41636:CDS
AGAGTAGATGGATTGGAGAG+AGG 0.627060 3.4:+88369811 None:intergenic
TTGTTTGGAAGCTACACGCA+TGG 0.631363 3.4:+88369705 None:intergenic
TTTGTGGTGCTCCCAAACGA+AGG 0.633347 3.4:-88368933 MS.gene41636:CDS
TGCAAGAACTACAACTCCCA+CGG 0.635141 3.4:+88368692 None:intergenic
TATCAGGCAATTTATCAAAG+GGG 0.660862 3.4:+88369547 None:intergenic
GCTACACGCATGGTAAACCT+GGG 0.677062 3.4:+88369715 None:intergenic
TAATCTTGTAGTGTGAAGGG+AGG 0.698956 3.4:+88369861 None:intergenic
ACACGCATGGTAAACCTGGG+AGG 0.732150 3.4:+88369718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTATCAAATTCTTGT+TGG - chr3.4:88369135-88369154 MS.gene41636:intron 15.0%
!! ATGTCAAAAACAAAAATAAT+GGG + chr3.4:88369780-88369799 None:intergenic 15.0%
!! CATATCTATTATCTATATAT+AGG - chr3.4:88369267-88369286 MS.gene41636:intron 15.0%
!! GAAATATATATCTCATTATT+TGG - chr3.4:88368925-88368944 MS.gene41636:CDS 15.0%
!! GAATTTGATAAATATTCATA+TGG + chr3.4:88369131-88369150 None:intergenic 15.0%
!! TAATATTATACTGATAAGAA+AGG - chr3.4:88369500-88369519 MS.gene41636:intron 15.0%
!! TATGTCAAAAACAAAAATAA+TGG + chr3.4:88369781-88369800 None:intergenic 15.0%
!! AAAAAGTAATGAAATTACCA+GGG + chr3.4:88369028-88369047 None:intergenic 20.0%
!! AATTTAGACCTTAATAAACT+TGG + chr3.4:88369479-88369498 None:intergenic 20.0%
!! TAAAAAGTAATGAAATTACC+AGG + chr3.4:88369029-88369048 None:intergenic 20.0%
!! TAACAATTTCTATTGACATA+AGG - chr3.4:88369199-88369218 MS.gene41636:intron 20.0%
!! TTATCTATATATAGGATGTA+TGG - chr3.4:88369275-88369294 MS.gene41636:intron 20.0%
!! TTTAAGAAGCAAAAGAAATA+CGG - chr3.4:88369538-88369557 MS.gene41636:CDS 20.0%
!!! TTTTTCAACCAAGTTTATTA+AGG - chr3.4:88369468-88369487 MS.gene41636:intron 20.0%
! AAAAGAGAATGATCAAAGAA+AGG + chr3.4:88368891-88368910 None:intergenic 25.0%
! AAATGAAACAGATGTTAGAA+AGG - chr3.4:88369664-88369683 MS.gene41636:intron 25.0%
! ACTGATAAGAAAGGAAAAAT+AGG - chr3.4:88369509-88369528 MS.gene41636:intron 25.0%
! TGATAAATATTCATATGGCA+TGG + chr3.4:88369126-88369145 None:intergenic 25.0%
! TTAAGAAGCAAAAGAAATAC+GGG - chr3.4:88369539-88369558 MS.gene41636:CDS 25.0%
! TTATCAGGCAATTTATCAAA+GGG + chr3.4:88368996-88369015 None:intergenic 25.0%
! TTTATCAGGCAATTTATCAA+AGG + chr3.4:88368997-88369016 None:intergenic 25.0%
! TTTATTATGCTAATGTCAAC+AGG - chr3.4:88369802-88369821 MS.gene41636:CDS 25.0%
!!! CAAAAACAAAAATAATGGGT+AGG + chr3.4:88369776-88369795 None:intergenic 25.0%
!!! TCTGCTTTAAGTTCTAAATT+TGG + chr3.4:88369413-88369432 None:intergenic 25.0%
!!! TTTGTTGATAATAATGTTGC+AGG - chr3.4:88368949-88368968 MS.gene41636:intron 25.0%
AAGTACAGAGAAAGAAAATC+AGG - chr3.4:88369244-88369263 MS.gene41636:intron 30.0%
AATCAACTACAAGTTGCTTA+TGG - chr3.4:88368797-88368816 MS.gene41636:intron 30.0%
AGGATGAAGTAAAAGGTTAA+GGG + chr3.4:88368777-88368796 None:intergenic 30.0%
AGGCAAAAAGAAAATGACTA+TGG + chr3.4:88369736-88369755 None:intergenic 30.0%
GCATTATGATTGTCTCAAAA+TGG - chr3.4:88369573-88369592 MS.gene41636:intron 30.0%
GTTGATTAGGATGAAGTAAA+AGG + chr3.4:88368784-88368803 None:intergenic 30.0%
TAAGCAACTTGTAGTTGATT+AGG + chr3.4:88368797-88368816 None:intergenic 30.0%
TAGGATGAAGTAAAAGGTTA+AGG + chr3.4:88368778-88368797 None:intergenic 30.0%
TATCAGGCAATTTATCAAAG+GGG + chr3.4:88368995-88369014 None:intergenic 30.0%
TGATAATCTTGTAGTGTGAA+GGG + chr3.4:88368684-88368703 None:intergenic 30.0%
! TCTAAAATGTGTTGCCTATA+TGG + chr3.4:88369367-88369386 None:intergenic 30.0%
!! GCTTTAAGTTCTAAATTTGG+TGG + chr3.4:88369410-88369429 None:intergenic 30.0%
AAAATGTGTTGCCTATATGG+CGG + chr3.4:88369364-88369383 None:intergenic 35.0%
AAAGATGTCTTGAGAGTAGA+TGG + chr3.4:88368743-88368762 None:intergenic 35.0%
AGGAGTGAACATATTAAGCA+AGG + chr3.4:88369756-88369775 None:intergenic 35.0%
ATCAGGCAATTTATCAAAGG+GGG + chr3.4:88368994-88369013 None:intergenic 35.0%
ATGGATTCTAAGTGCATGTT+AGG - chr3.4:88369294-88369313 MS.gene41636:intron 35.0%
GCAGGTATATTTACAATGAG+AGG - chr3.4:88368967-88368986 MS.gene41636:intron 35.0%
GTGATAATCTTGTAGTGTGA+AGG + chr3.4:88368685-88368704 None:intergenic 35.0%
GTTAGGTTCAGTGAATTTGA+CGG - chr3.4:88369311-88369330 MS.gene41636:intron 35.0%
TCCAAAGAGATGAAGCAATT+GGG - chr3.4:88369702-88369721 MS.gene41636:CDS 35.0%
TGTCCACTATTACTCATAGA+TGG - chr3.4:88369058-88369077 MS.gene41636:intron 35.0%
TTGTTGACATCTGTTGCATA+GGG + chr3.4:88369638-88369657 None:intergenic 35.0%
TTTGTTGACATCTGTTGCAT+AGG + chr3.4:88369639-88369658 None:intergenic 35.0%
! ACGACAGATATAAGCTTGTT+TGG + chr3.4:88368852-88368871 None:intergenic 35.0%
ACCCAATTGCTTCATCTCTT+TGG + chr3.4:88369706-88369725 None:intergenic 40.0%
ATAGGGCTTAAACCTTCGTT+TGG + chr3.4:88369621-88369640 None:intergenic 40.0%
CCACTATTACTCATAGATGG+AGG - chr3.4:88369061-88369080 MS.gene41636:intron 40.0%
CCTCCATCTATGAGTAATAG+TGG + chr3.4:88369064-88369083 None:intergenic 40.0%
CGGTGAATTTGACAGAATCA+CGG - chr3.4:88369331-88369350 MS.gene41636:intron 40.0%
CTCCAAAGAGATGAAGCAAT+TGG - chr3.4:88369701-88369720 MS.gene41636:CDS 40.0%
TAATCTTGTAGTGTGAAGGG+AGG + chr3.4:88368681-88368700 None:intergenic 40.0%
TAGGGCTTAAACCTTCGTTT+GGG + chr3.4:88369620-88369639 None:intergenic 40.0%
TGAATTTGACAGAATCACGG+TGG - chr3.4:88369334-88369353 MS.gene41636:intron 40.0%
TGTCTTGAGAGTAGATGGAT+TGG + chr3.4:88368738-88368757 None:intergenic 40.0%
! AAATGGCGTTTTGCAGTTTG+TGG - chr3.4:88369590-88369609 MS.gene41636:intron 40.0%
ATGAGACCAGTCTTTGTGAG+TGG + chr3.4:88369445-88369464 None:intergenic 45.0%
CCTGATAAATACTTCTGCCC+TGG - chr3.4:88369008-88369027 MS.gene41636:intron 45.0%
CTTATATCTGTCGTGACTGC+GGG - chr3.4:88368858-88368877 MS.gene41636:CDS 45.0%
GCTTATATCTGTCGTGACTG+CGG - chr3.4:88368857-88368876 MS.gene41636:CDS 45.0%
TGCAAGAACTACAACTCCCA+CGG + chr3.4:88369850-88369869 None:intergenic 45.0%
TTGTTTGGAAGCTACACGCA+TGG + chr3.4:88368837-88368856 None:intergenic 45.0%
!! AGAGTAGATGGATTGGAGAG+AGG + chr3.4:88368731-88368750 None:intergenic 45.0%
!! CCAGGGCAGAAGTATTTATC+AGG + chr3.4:88369011-88369030 None:intergenic 45.0%
!! TAGATGGATTGGAGAGAGGT+CGG + chr3.4:88368727-88368746 None:intergenic 45.0%
AAGTTGCTTATGGACCTCCC+AGG - chr3.4:88368807-88368826 MS.gene41636:intron 50.0%
ACTTCCTATTGCAGTTGCCG+TGG - chr3.4:88369830-88369849 MS.gene41636:CDS 50.0%
AGCTACACGCATGGTAAACC+TGG + chr3.4:88368828-88368847 None:intergenic 50.0%
CTTCCTATTGCAGTTGCCGT+GGG - chr3.4:88369831-88369850 MS.gene41636:CDS 50.0%
GCTACACGCATGGTAAACCT+GGG + chr3.4:88368827-88368846 None:intergenic 50.0%
GTGGGAGTTGTAGTTCTTGC+AGG - chr3.4:88369849-88369868 MS.gene41636:CDS 50.0%
TCACTGCCACTCACAAAGAC+TGG - chr3.4:88369436-88369455 MS.gene41636:intron 50.0%
TTTGTGGTGCTCCCAAACGA+AGG - chr3.4:88369606-88369625 MS.gene41636:intron 50.0%
!! ACGGTGGATTACCGCCATAT+AGG - chr3.4:88369350-88369369 MS.gene41636:intron 50.0%
ACACGCATGGTAAACCTGGG+AGG + chr3.4:88368824-88368843 None:intergenic 55.0%
ACTCCCACGGCAACTGCAAT+AGG + chr3.4:88369837-88369856 None:intergenic 55.0%
! TGGATTGGAGAGAGGTCGGA+GGG + chr3.4:88368723-88368742 None:intergenic 55.0%
!! ATGGATTGGAGAGAGGTCGG+AGG + chr3.4:88368724-88368743 None:intergenic 55.0%
AAGGGAGGCCTGCACTTGCA+AGG + chr3.4:88368666-88368685 None:intergenic 60.0%
TTGGAGAGAGGTCGGAGGGT+AGG + chr3.4:88368719-88368738 None:intergenic 60.0%
CGGAGGGTAGGAGTGGCAGT+AGG + chr3.4:88368707-88368726 None:intergenic 65.0%
GAGAGGTCGGAGGGTAGGAG+TGG + chr3.4:88368714-88368733 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 88368659 88369902 88368659 ID=MS.gene41636
chr3.4 mRNA 88368659 88369902 88368659 ID=MS.gene41636.t1;Parent=MS.gene41636
chr3.4 exon 88369682 88369902 88369682 ID=MS.gene41636.t1.exon1;Parent=MS.gene41636.t1
chr3.4 CDS 88369682 88369902 88369682 ID=cds.MS.gene41636.t1;Parent=MS.gene41636.t1
chr3.4 exon 88369532 88369590 88369532 ID=MS.gene41636.t1.exon2;Parent=MS.gene41636.t1
chr3.4 CDS 88369532 88369590 88369532 ID=cds.MS.gene41636.t1;Parent=MS.gene41636.t1
chr3.4 exon 88368838 88368955 88368838 ID=MS.gene41636.t1.exon3;Parent=MS.gene41636.t1
chr3.4 CDS 88368838 88368955 88368838 ID=cds.MS.gene41636.t1;Parent=MS.gene41636.t1
chr3.4 exon 88368659 88368737 88368659 ID=MS.gene41636.t1.exon4;Parent=MS.gene41636.t1
chr3.4 CDS 88368659 88368737 88368659 ID=cds.MS.gene41636.t1;Parent=MS.gene41636.t1
Gene Sequence

>MS.gene41636

ATGGCCTTGCAAGTGCAGGCCTCCCTTCACACTACAAGATTATCACCTACTGCCACTCCTACCCTCCGACCTCTCTCCAATCCATCTACTCTCAAGACATCTTTCTTCTCTCGTTCCCTTAACCTTTTACTTCATCCTAATCAACTACAAGTTGCTTATGGACCTCCCAGGTTTACCATGCGTGTAGCTTCCAAACAAGCTTATATCTGTCGTGACTGCGGGTTATTTTCCTTTCTTTGATCATTCTCTTTTTAGTATATCTGAATGAAATATATATCTCATTATTTGGTTTTGTTGATAATAATGTTGCAGGTATATTTACAATGAGAGGACCCCCTTTGATAAATTGCCTGATAAATACTTCTGCCCTGGTAATTTCATTACTTTTTACTCATATAGTGTCCACTATTACTCATAGATGGAGGAACTTCATTTCAAAACTATAATATTTGCTTTTACGCTTTCCATGCCATATGAATATTTATCAAATTCTTGTTGGTTTAGTTGTCTAACAAAAAATAAACAATTTTATCATGATCATAACAATTTCTATTGACATAAGGTATACTATTAAATTTCTCTTATAAGTACAGAGAAAGAAAATCAGGCATATCTATTATCTATATATAGGATGTATGGATTCTAAGTGCATGTTAGGTTCAGTGAATTTGACGGTGAATTTGACAGAATCACGGTGGATTACCGCCATATAGGCAACACATTTTAGAGTTTCAAAAAAATCATAGTGCCACCAAATTTAGAACTTAAAGCAGAGCTTCACTGCCACTCACAAAGACTGGTCTCATTTGTTTTTCAACCAAGTTTATTAAGGTCTAAATTCTAATATTATACTGATAAGAAAGGAAAAATAGGACATTTTTTAAGAAGCAAAAGAAATACGGGTCAATGAGACTGCATTATGATTGTCTCAAAATGGCGTTTTGCAGTTTGTGGTGCTCCCAAACGAAGGTTTAAGCCCTATGCAACAGATGTCAACAAAAAAGCAAATGAAACAGATGTTAGAAAGGCAAGAAAAGCTGAACTCCAAAGAGATGAAGCAATTGGGTGAGTGAGCCATAGTCATTTTCTTTTTGCCTTGCTTAATATGTTCACTCCTACCCATTATTTTTGTTTTTGACATAATTTATTATGCTAATGTCAACAGGAAAGCACTTCCTATTGCAGTTGCCGTGGGAGTTGTAGTTCTTGCAGGATTATACTTCTATCTCAACAACACATTTTAG

Protein sequence

>MS.gene41636.t1

MALQVQASLHTTRLSPTATPTLRPLSNPSTLKTSFFSRSLNLLLHPNQLQVAYGPPRFTMRVASKQAYICRDCGYIYNERTPFDKLPDKYFCPVCGAPKRRFKPYATDVNKKANETDVRKARKAELQRDEAIGKALPIAVAVGVVVLAGLYFYLNNTF