AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91915


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91915.t1 MTR_5g038970 94.472 199 10 1 1 198 1 199 6.07e-135 376
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91915.t1 AT1G14345 78.417 139 28 2 61 198 59 196 1.86e-76 228

Find 69 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAAGAATTCTCAGGTTT+TGG 0.237091 5.3:-27363732 MS.gene91915:CDS
CAGGTTGTTCCTAGGTTTAT+TGG 0.243976 5.3:-27363857 MS.gene91915:CDS
CTAAGAGTGAGTGTTATTTC+AGG 0.245187 5.3:-27363932 MS.gene91915:CDS
TGTGGATGTGGTAGGGGTTG+TGG 0.247060 5.3:+27364120 None:intergenic
GTGCACTTTACCTACGTTAC+AGG 0.269722 5.3:-27363964 MS.gene91915:CDS
GTGGATGTGGTAGGGGTTGT+GGG 0.271455 5.3:+27364121 None:intergenic
GGTGTGGTTGTGGTGGTTGA+TGG 0.281173 5.3:+27364234 None:intergenic
CTGAAATAACACTCACTCTT+AGG 0.291915 5.3:+27363933 None:intergenic
GGGGTTGTGGGCTTGTTTGT+TGG 0.299097 5.3:+27364133 None:intergenic
TTTACGGTTGTGGGTGGAGG+TGG 0.315642 5.3:+27364186 None:intergenic
TTCCTAGGTTTATTGGTGAA+TGG 0.317822 5.3:-27363850 MS.gene91915:CDS
GGGGTTGGAGGGTGTGGTTG+TGG 0.332045 5.3:+27364224 None:intergenic
TGGTTGATGGTGGTGGTTGT+TGG 0.333417 5.3:+27364247 None:intergenic
GTTGGAGGGTGTGGTTGTGG+TGG 0.352309 5.3:+27364227 None:intergenic
GTGGTTGTGTGGATGTGGTA+GGG 0.363059 5.3:+27364113 None:intergenic
TAGTGTTATCAAAGATGTTC+AGG 0.370339 5.3:-27363876 MS.gene91915:CDS
TGTGGTGAGTTTACGGTTGT+GGG 0.371948 5.3:+27364177 None:intergenic
AAGAAGATGGTTTCTGCATT+GGG 0.372772 5.3:+27364079 None:intergenic
TTGTGGTGAGTTTACGGTTG+TGG 0.379941 5.3:+27364176 None:intergenic
GGTGGTTGTGTGGATGTGGT+AGG 0.382236 5.3:+27364112 None:intergenic
AAAATAAATCAAAAGCCTTT+AGG 0.382275 5.3:+27363698 None:intergenic
TCCTAGGTTTATTGGTGAAT+GGG 0.386223 5.3:-27363849 MS.gene91915:CDS
TGAAACTGCTGCTAGTGTTA+AGG 0.412622 5.3:+27363988 None:intergenic
GGAAGAGGGGTTGGAGGGTG+TGG 0.412714 5.3:+27364218 None:intergenic
AGTGTTATTTCAGGCTTAAC+GGG 0.414099 5.3:-27363923 MS.gene91915:CDS
GAAACTGCTGCTAGTGTTAA+GGG 0.418053 5.3:+27363989 None:intergenic
GAGTGTTATTTCAGGCTTAA+CGG 0.418536 5.3:-27363924 MS.gene91915:CDS
TGTGGGCTTGTTTGTTGGTG+AGG 0.420960 5.3:+27364138 None:intergenic
TGGTTGTGTGGATGTGGTAG+GGG 0.423031 5.3:+27364114 None:intergenic
TTAGCAATTTCTCTAAACTT+AGG 0.423972 5.3:+27363773 None:intergenic
TAAGTATTGTTTGTCTATGA+AGG 0.424827 5.3:-27363753 MS.gene91915:CDS
TTACGGTTGTGGGTGGAGGT+GGG 0.431500 5.3:+27364187 None:intergenic
TACTCGGCTTCACTCTTGTA+TGG 0.433021 5.3:-27364015 MS.gene91915:CDS
GAGTTTACGGTTGTGGGTGG+AGG 0.435485 5.3:+27364183 None:intergenic
TTGATGGTGGTGGTTGTTGG+TGG 0.448728 5.3:+27364250 None:intergenic
TTGGATCAATCTGGTCCTAA+AGG 0.450236 5.3:-27363713 MS.gene91915:CDS
ATGGTACAAAGGTTGATCTT+AGG 0.451687 5.3:-27363802 MS.gene91915:CDS
GAAGAAGATGGTTTCTGCAT+TGG 0.454026 5.3:+27364078 None:intergenic
GTTTGATGAGGAAGAGGGGT+TGG 0.457551 5.3:+27364209 None:intergenic
AGGTGGTGGTTGTGTGGATG+TGG 0.459887 5.3:+27364108 None:intergenic
GTGGTTGTGGTGGTTGATGG+TGG 0.462739 5.3:+27364237 None:intergenic
TGATGAGGAAGAGGGGTTGG+AGG 0.465797 5.3:+27364212 None:intergenic
ATTGTTATTACATTAAAAGA+TGG 0.465933 5.3:-27363821 MS.gene91915:CDS
GTTGTGGTGGTTGATGGTGG+TGG 0.466674 5.3:+27364240 None:intergenic
GGTGGGTTTGATGAGGAAGA+GGG 0.474274 5.3:+27364204 None:intergenic
GATGAGGAAGAGGGGTTGGA+GGG 0.483645 5.3:+27364213 None:intergenic
TATGAAGGAGAAGAATTCTC+AGG 0.492519 5.3:-27363738 MS.gene91915:CDS
GGGTGGAGGTGGGTTTGATG+AGG 0.494426 5.3:+27364197 None:intergenic
AAGATGTTCAGGTTGTTCCT+AGG 0.508304 5.3:-27363865 MS.gene91915:CDS
CTCATCACCAACCTCCTACT+CGG 0.528547 5.3:-27364031 MS.gene91915:CDS
AGGTGGGTTTGATGAGGAAG+AGG 0.533100 5.3:+27364203 None:intergenic
GGTGAGTTTACGGTTGTGGG+TGG 0.544615 5.3:+27364180 None:intergenic
TATTTCAGGCTTAACGGGTG+AGG 0.547577 5.3:-27363918 MS.gene91915:CDS
AGCTCCACCGTCGAAGAAGA+TGG 0.550541 5.3:+27364066 None:intergenic
TACATTAAAAGATGGTACAA+AGG 0.555819 5.3:-27363813 MS.gene91915:CDS
GAAACCATCTTCTTCGACGG+TGG 0.561109 5.3:-27364070 MS.gene91915:CDS
CGAGTAGGAGGTTGGTGATG+AGG 0.561148 5.3:+27364032 None:intergenic
CCTAGGTTTATTGGTGAATG+GGG 0.564165 5.3:-27363848 MS.gene91915:CDS
GTGGGTTTGATGAGGAAGAG+GGG 0.565343 5.3:+27364205 None:intergenic
CAAGAGTGAAGCCGAGTAGG+AGG 0.565864 5.3:+27364020 None:intergenic
TTCGACGGTGGAGCTCACTA+CGG 0.570521 5.3:-27364058 MS.gene91915:CDS
AGTGAAGCCGAGTAGGAGGT+TGG 0.574847 5.3:+27364024 None:intergenic
AATAACACTCACTCTTAGGT+TGG 0.579943 5.3:+27363937 None:intergenic
ATACAAGAGTGAAGCCGAGT+AGG 0.584381 5.3:+27364017 None:intergenic
GCAGAAACCATCTTCTTCGA+CGG 0.614487 5.3:-27364073 MS.gene91915:CDS
GGTTGGTGAACCTGTAACGT+AGG 0.615503 5.3:+27363954 None:intergenic
GTTGTTGGTGGTGATAAGAG+AGG 0.626805 5.3:+27364262 None:intergenic
TTGTTGGTGAGGATGAGACG+TGG 0.634180 5.3:+27364149 None:intergenic
CCCCATTCACCAATAAACCT+AGG 0.651079 5.3:+27363848 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGTTATTACATTAAAAGA+TGG - chr5.3:27364165-27364184 MS.gene91915:CDS 15.0%
! TACATTAAAAGATGGTACAA+AGG - chr5.3:27364173-27364192 MS.gene91915:CDS 25.0%
! TTAGCAATTTCTCTAAACTT+AGG + chr5.3:27364216-27364235 None:intergenic 25.0%
!! TAAGTATTGTTTGTCTATGA+AGG - chr5.3:27364233-27364252 MS.gene91915:CDS 25.0%
TAGTGTTATCAAAGATGTTC+AGG - chr5.3:27364110-27364129 MS.gene91915:CDS 30.0%
AAGAAGATGGTTTCTGCATT+GGG + chr5.3:27363910-27363929 None:intergenic 35.0%
AATAACACTCACTCTTAGGT+TGG + chr5.3:27364052-27364071 None:intergenic 35.0%
CTGAAATAACACTCACTCTT+AGG + chr5.3:27364056-27364075 None:intergenic 35.0%
TATGAAGGAGAAGAATTCTC+AGG - chr5.3:27364248-27364267 MS.gene91915:CDS 35.0%
TCCTAGGTTTATTGGTGAAT+GGG - chr5.3:27364137-27364156 MS.gene91915:CDS 35.0%
TTCCTAGGTTTATTGGTGAA+TGG - chr5.3:27364136-27364155 MS.gene91915:CDS 35.0%
! ATGGTACAAAGGTTGATCTT+AGG - chr5.3:27364184-27364203 MS.gene91915:CDS 35.0%
!! AGTGTTATTTCAGGCTTAAC+GGG - chr5.3:27364063-27364082 MS.gene91915:CDS 35.0%
!! CTAAGAGTGAGTGTTATTTC+AGG - chr5.3:27364054-27364073 MS.gene91915:CDS 35.0%
!! GAGTGTTATTTCAGGCTTAA+CGG - chr5.3:27364062-27364081 MS.gene91915:CDS 35.0%
CAGGTTGTTCCTAGGTTTAT+TGG - chr5.3:27364129-27364148 MS.gene91915:CDS 40.0%
CCTAGGTTTATTGGTGAATG+GGG - chr5.3:27364138-27364157 MS.gene91915:CDS 40.0%
GAAACTGCTGCTAGTGTTAA+GGG + chr5.3:27364000-27364019 None:intergenic 40.0%
GAAGAAGATGGTTTCTGCAT+TGG + chr5.3:27363911-27363930 None:intergenic 40.0%
TGAAACTGCTGCTAGTGTTA+AGG + chr5.3:27364001-27364020 None:intergenic 40.0%
TTGGATCAATCTGGTCCTAA+AGG - chr5.3:27364273-27364292 MS.gene91915:CDS 40.0%
! GGAGAAGAATTCTCAGGTTT+TGG - chr5.3:27364254-27364273 MS.gene91915:CDS 40.0%
!! AAGATGTTCAGGTTGTTCCT+AGG - chr5.3:27364121-27364140 MS.gene91915:CDS 40.0%
!! GGAGTTTTGTGGTGAGTTTA+CGG + chr5.3:27363819-27363838 None:intergenic 40.0%
!!! TCTCAGGTTTTGGATCAATC+TGG - chr5.3:27364264-27364283 MS.gene91915:CDS 40.0%
ATACAAGAGTGAAGCCGAGT+AGG + chr5.3:27363972-27363991 None:intergenic 45.0%
CCCCATTCACCAATAAACCT+AGG + chr5.3:27364141-27364160 None:intergenic 45.0%
GCAGAAACCATCTTCTTCGA+CGG - chr5.3:27363913-27363932 MS.gene91915:CDS 45.0%
GTGCACTTTACCTACGTTAC+AGG - chr5.3:27364022-27364041 MS.gene91915:CDS 45.0%
TACTCGGCTTCACTCTTGTA+TGG - chr5.3:27363971-27363990 MS.gene91915:CDS 45.0%
TGTGGTGAGTTTACGGTTGT+GGG + chr5.3:27363812-27363831 None:intergenic 45.0%
TTGTGGTGAGTTTACGGTTG+TGG + chr5.3:27363813-27363832 None:intergenic 45.0%
! GTTTCTGCATTGGGCTTTTG+AGG + chr5.3:27363901-27363920 None:intergenic 45.0%
! TATTTCAGGCTTAACGGGTG+AGG - chr5.3:27364068-27364087 MS.gene91915:CDS 45.0%
!! GTTGTTGGTGGTGATAAGAG+AGG + chr5.3:27363727-27363746 None:intergenic 45.0%
CTCATCACCAACCTCCTACT+CGG - chr5.3:27363955-27363974 MS.gene91915:CDS 50.0%
GAAACCATCTTCTTCGACGG+TGG - chr5.3:27363916-27363935 MS.gene91915:CDS 50.0%
GTGGGTTTGATGAGGAAGAG+GGG + chr5.3:27363784-27363803 None:intergenic 50.0%
GTGGTTGTGTGGATGTGGTA+GGG + chr5.3:27363876-27363895 None:intergenic 50.0%
GTTTGATGAGGAAGAGGGGT+TGG + chr5.3:27363780-27363799 None:intergenic 50.0%
TGGTTGTGTGGATGTGGTAG+GGG + chr5.3:27363875-27363894 None:intergenic 50.0%
! AGGTGGGTTTGATGAGGAAG+AGG + chr5.3:27363786-27363805 None:intergenic 50.0%
! GGATGAGACGTGGAGTTTTG+TGG + chr5.3:27363830-27363849 None:intergenic 50.0%
! GGTGGGTTTGATGAGGAAGA+GGG + chr5.3:27363785-27363804 None:intergenic 50.0%
! TGTGGGCTTGTTTGTTGGTG+AGG + chr5.3:27363851-27363870 None:intergenic 50.0%
! TTGTTGGTGAGGATGAGACG+TGG + chr5.3:27363840-27363859 None:intergenic 50.0%
!! GGTTGGTGAACCTGTAACGT+AGG + chr5.3:27364035-27364054 None:intergenic 50.0%
!! TCTGCATTGGGCTTTTGAGG+TGG + chr5.3:27363898-27363917 None:intergenic 50.0%
!! TGGTTGATGGTGGTGGTTGT+TGG + chr5.3:27363742-27363761 None:intergenic 50.0%
!! TTGATGGTGGTGGTTGTTGG+TGG + chr5.3:27363739-27363758 None:intergenic 50.0%
!!! CTTTTGAGGTGGTGGTTGTG+TGG + chr5.3:27363887-27363906 None:intergenic 50.0%
AGCTCCACCGTCGAAGAAGA+TGG + chr5.3:27363923-27363942 None:intergenic 55.0%
AGTGAAGCCGAGTAGGAGGT+TGG + chr5.3:27363965-27363984 None:intergenic 55.0%
CAAGAGTGAAGCCGAGTAGG+AGG + chr5.3:27363969-27363988 None:intergenic 55.0%
GAGTTTACGGTTGTGGGTGG+AGG + chr5.3:27363806-27363825 None:intergenic 55.0%
GATGAGGAAGAGGGGTTGGA+GGG + chr5.3:27363776-27363795 None:intergenic 55.0%
GGTGAGTTTACGGTTGTGGG+TGG + chr5.3:27363809-27363828 None:intergenic 55.0%
GGTGGTTGTGTGGATGTGGT+AGG + chr5.3:27363877-27363896 None:intergenic 55.0%
GGTGTGGTTGTGGTGGTTGA+TGG + chr5.3:27363755-27363774 None:intergenic 55.0%
GTGGATGTGGTAGGGGTTGT+GGG + chr5.3:27363868-27363887 None:intergenic 55.0%
TGATGAGGAAGAGGGGTTGG+AGG + chr5.3:27363777-27363796 None:intergenic 55.0%
TGTGGATGTGGTAGGGGTTG+TGG + chr5.3:27363869-27363888 None:intergenic 55.0%
TTACGGTTGTGGGTGGAGGT+GGG + chr5.3:27363802-27363821 None:intergenic 55.0%
TTCGACGGTGGAGCTCACTA+CGG - chr5.3:27363928-27363947 MS.gene91915:CDS 55.0%
TTTACGGTTGTGGGTGGAGG+TGG + chr5.3:27363803-27363822 None:intergenic 55.0%
! AGGTGGTGGTTGTGTGGATG+TGG + chr5.3:27363881-27363900 None:intergenic 55.0%
! GGGGTTGTGGGCTTGTTTGT+TGG + chr5.3:27363856-27363875 None:intergenic 55.0%
!! CGAGTAGGAGGTTGGTGATG+AGG + chr5.3:27363957-27363976 None:intergenic 55.0%
!! GTGGTTGTGGTGGTTGATGG+TGG + chr5.3:27363752-27363771 None:intergenic 55.0%
!! GTTGTGGTGGTTGATGGTGG+TGG + chr5.3:27363749-27363768 None:intergenic 55.0%
!!! GCATTGGGCTTTTGAGGTGG+TGG + chr5.3:27363895-27363914 None:intergenic 55.0%
! GGGTGGAGGTGGGTTTGATG+AGG + chr5.3:27363792-27363811 None:intergenic 60.0%
! GTTGGAGGGTGTGGTTGTGG+TGG + chr5.3:27363762-27363781 None:intergenic 60.0%
GGAAGAGGGGTTGGAGGGTG+TGG + chr5.3:27363771-27363790 None:intergenic 65.0%
! GGGGTTGGAGGGTGTGGTTG+TGG + chr5.3:27363765-27363784 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 27363706 27364302 27363706 ID=MS.gene91915
chr5.3 mRNA 27363706 27364302 27363706 ID=MS.gene91915.t1;Parent=MS.gene91915
chr5.3 exon 27363706 27364302 27363706 ID=MS.gene91915.t1.exon1;Parent=MS.gene91915.t1
chr5.3 CDS 27363706 27364302 27363706 ID=cds.MS.gene91915.t1;Parent=MS.gene91915.t1
Gene Sequence

>MS.gene91915

ATGGCTTGCAATTCTACACCTCTCTTATCACCACCAACAACCACCACCATCAACCACCACAACCACACCCTCCAACCCCTCTTCCTCATCAAACCCACCTCCACCCACAACCGTAAACTCACCACAAAACTCCACGTCTCATCCTCACCAACAAACAAGCCCACAACCCCTACCACATCCACACAACCACCACCTCAAAAGCCCAATGCAGAAACCATCTTCTTCGACGGTGGAGCTCACTACGGAGACCTCATCACCAACCTCCTACTCGGCTTCACTCTTGTATGGCTTCCCTTAACACTAGCAGCAGTTTCACGTGCACTTTACCTACGTTACAGGTTCACCAACCTAAGAGTGAGTGTTATTTCAGGCTTAACGGGTGAGGATAGAAGTGACTTTTCATATAGTGTTATCAAAGATGTTCAGGTTGTTCCTAGGTTTATTGGTGAATGGGGTGATATTGTTATTACATTAAAAGATGGTACAAAGGTTGATCTTAGGAGTGTTCCTAAGTTTAGAGAAATTGCTAAGTATTGTTTGTCTATGAAGGAGAAGAATTCTCAGGTTTTGGATCAATCTGGTCCTAAAGGCTTTTGA

Protein sequence

>MS.gene91915.t1

MACNSTPLLSPPTTTTINHHNHTLQPLFLIKPTSTHNRKLTTKLHVSSSPTNKPTTPTTSTQPPPQKPNAETIFFDGGAHYGDLITNLLLGFTLVWLPLTLAAVSRALYLRYRFTNLRVSVISGLTGEDRSDFSYSVIKDVQVVPRFIGEWGDIVITLKDGTKVDLRSVPKFREIAKYCLSMKEKNSQVLDQSGPKGF