AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72999


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72999.t1 MTR_4g103330 95.187 187 9 0 1 187 62 248 1.41e-119 338
MS.gene72999.t1 MTR_7g117900 29.197 137 89 3 6 141 82 211 1.60e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72999.t1 AT4G09650 63.102 187 69 0 1 187 47 233 2.66e-84 249

Find 45 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCGTAGCTGGCGGCGGC+TGG 0.162799 8.3:+18910585 None:intergenic
ACCGTCAGGTATGGGAATTC+TGG 0.172359 8.3:-18910138 MS.gene72999:CDS
TTGGTGGAGTCTTCGATAAT+TGG 0.238527 8.3:+18910450 None:intergenic
AATTGCTGCTCAACTTGATT+TGG 0.271511 8.3:-18910073 MS.gene72999:CDS
TGGCGTAGCTGGCGGCGGCT+GGG 0.280536 8.3:+18910586 None:intergenic
AACTGCTCTTGATCCAAGTT+TGG 0.310216 8.3:-18910172 MS.gene72999:CDS
TTTAGCGCAGATTGCGAAAC+AGG 0.332243 8.3:-18910232 MS.gene72999:CDS
GTCGAGGGTGTTGTTGGATT+TGG 0.360334 8.3:+18910545 None:intergenic
GAGAAATAGTCGAACACCTT+GGG 0.363815 8.3:+18910477 None:intergenic
ATTGCTGCTCAACTTGATTT+GGG 0.372073 8.3:-18910072 MS.gene72999:CDS
CCGTCAGGTATGGGAATTCT+GGG 0.374285 8.3:-18910137 MS.gene72999:CDS
GGTGTCTTCAGTGGTGAAAT+TGG 0.383089 8.3:-18910265 MS.gene72999:CDS
GGCTAATGCGGTGGCGTAGC+TGG 0.387942 8.3:+18910575 None:intergenic
AGCGATGAGTTTGCGTTTGG+TGG 0.395419 8.3:+18910434 None:intergenic
GTAAAACCCGCCACCAAACT+TGG 0.397977 8.3:+18910159 None:intergenic
TGGTGGAGTCTTCGATAATT+GGG 0.398456 8.3:+18910451 None:intergenic
GGCGTAGCTGGCGGCGGCTG+GGG 0.401952 8.3:+18910587 None:intergenic
GTTGTTGGATTTGGCTAAAT+CGG 0.409703 8.3:+18910554 None:intergenic
TTCAGCGATGAGTTTGCGTT+TGG 0.420804 8.3:+18910431 None:intergenic
GGTGGCGTCGAGGGTGTTGT+TGG 0.434585 8.3:+18910539 None:intergenic
TGCGGTGGCGTAGCTGGCGG+CGG 0.438767 8.3:+18910581 None:intergenic
TTTGTCGATGTCAGCGGTGG+TGG 0.445558 8.3:+18910521 None:intergenic
GTCAGCGGTGGTGGCGTCGA+GGG 0.465818 8.3:+18910530 None:intergenic
CTTGATCCAAGTTTGGTGGC+GGG 0.474377 8.3:-18910165 MS.gene72999:CDS
TGCTCTTGATCCAAGTTTGG+TGG 0.485731 8.3:-18910169 MS.gene72999:CDS
TCTTGATCCAAGTTTGGTGG+CGG 0.512469 8.3:-18910166 MS.gene72999:CDS
TTCTGTTTGCTTCGATGAGG+AGG 0.515130 8.3:+18910361 None:intergenic
TTGCTTCGATGAGGAGGTTG+AGG 0.520100 8.3:+18910367 None:intergenic
GGAGTCTTCGATAATTGGGC+TGG 0.533217 8.3:+18910455 None:intergenic
GGAGAAATAGTCGAACACCT+TGG 0.545934 8.3:+18910476 None:intergenic
CGATTCTGTTTGCTTCGATG+AGG 0.552223 8.3:+18910358 None:intergenic
TAATGCGGTGGCGTAGCTGG+CGG 0.559050 8.3:+18910578 None:intergenic
GGCTAAATCGGCTAATGCGG+TGG 0.571556 8.3:+18910566 None:intergenic
GCTTGTTGTGGTGTCTTCAG+TGG 0.590381 8.3:-18910274 MS.gene72999:CDS
TGTCAGCGGTGGTGGCGTCG+AGG 0.591340 8.3:+18910529 None:intergenic
AGAACTCTTCACTGACCCCA+AGG 0.615212 8.3:-18910493 MS.gene72999:CDS
CGAATTCTCTCACGATGTCG+AGG 0.616657 8.3:+18910328 None:intergenic
TTTGGCTAAATCGGCTAATG+CGG 0.625107 8.3:+18910563 None:intergenic
AACCGACACAGAGCTTGTTG+TGG 0.631106 8.3:-18910286 MS.gene72999:CDS
TCACAGGGGCGAAGAATGTG+AGG 0.634295 8.3:-18910200 MS.gene72999:CDS
GAGTGTTAAGAAGAAACTTG+AGG 0.637701 8.3:-18910097 MS.gene72999:CDS
CACCACAACAAGCTCTGTGT+CGG 0.640807 8.3:+18910284 None:intergenic
CCCAGAATTCCCATACCTGA+CGG 0.678407 8.3:+18910137 None:intergenic
TGTGTGTGGTTGGAAGCTAG+AGG 0.683601 8.3:+18910398 None:intergenic
AGAAATAGTCGAACACCTTG+GGG 0.687282 8.3:+18910478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAACAGGTTCAAAAACTCAC+AGG - chr8.3:18910428-18910447 MS.gene72999:CDS 35.0%
AACAGGTTCAAAAACTCACA+GGG - chr8.3:18910429-18910448 MS.gene72999:CDS 35.0%
AATTGCTGCTCAACTTGATT+TGG - chr8.3:18910571-18910590 MS.gene72999:CDS 35.0%
ATTGCTGCTCAACTTGATTT+GGG - chr8.3:18910572-18910591 MS.gene72999:CDS 35.0%
GAGTGTTAAGAAGAAACTTG+AGG - chr8.3:18910547-18910566 MS.gene72999:CDS 35.0%
GTTGTTGGATTTGGCTAAAT+CGG + chr8.3:18910093-18910112 None:intergenic 35.0%
AACTGCTCTTGATCCAAGTT+TGG - chr8.3:18910472-18910491 MS.gene72999:CDS 40.0%
ACAGGTTCAAAAACTCACAG+GGG - chr8.3:18910430-18910449 MS.gene72999:CDS 40.0%
AGAAATAGTCGAACACCTTG+GGG + chr8.3:18910169-18910188 None:intergenic 40.0%
GAGAAATAGTCGAACACCTT+GGG + chr8.3:18910170-18910189 None:intergenic 40.0%
TGGTGGAGTCTTCGATAATT+GGG + chr8.3:18910196-18910215 None:intergenic 40.0%
TTGGTGGAGTCTTCGATAAT+TGG + chr8.3:18910197-18910216 None:intergenic 40.0%
TTTGGCTAAATCGGCTAATG+CGG + chr8.3:18910084-18910103 None:intergenic 40.0%
! TTCGATTTTGTCGATGTCAG+CGG + chr8.3:18910132-18910151 None:intergenic 40.0%
!! TTGAGGAAGTTTTGTGTGTG+TGG + chr8.3:18910263-18910282 None:intergenic 40.0%
CGATTCTGTTTGCTTCGATG+AGG + chr8.3:18910289-18910308 None:intergenic 45.0%
GGAGAAATAGTCGAACACCT+TGG + chr8.3:18910171-18910190 None:intergenic 45.0%
GGTGTCTTCAGTGGTGAAAT+TGG - chr8.3:18910379-18910398 MS.gene72999:CDS 45.0%
TGCTCTTGATCCAAGTTTGG+TGG - chr8.3:18910475-18910494 MS.gene72999:CDS 45.0%
TTCAGCGATGAGTTTGCGTT+TGG + chr8.3:18910216-18910235 None:intergenic 45.0%
TTCTGTTTGCTTCGATGAGG+AGG + chr8.3:18910286-18910305 None:intergenic 45.0%
TTTAGCGCAGATTGCGAAAC+AGG - chr8.3:18910412-18910431 MS.gene72999:CDS 45.0%
! TCTTGATCCAAGTTTGGTGG+CGG - chr8.3:18910478-18910497 MS.gene72999:CDS 45.0%
!! GGAAGTTTTGTGTGTGTGGT+TGG + chr8.3:18910259-18910278 None:intergenic 45.0%
ACCGTCAGGTATGGGAATTC+TGG - chr8.3:18910506-18910525 MS.gene72999:CDS 50.0%
AGAACTCTTCACTGACCCCA+AGG - chr8.3:18910151-18910170 MS.gene72999:CDS 50.0%
AGCGATGAGTTTGCGTTTGG+TGG + chr8.3:18910213-18910232 None:intergenic 50.0%
CACCACAACAAGCTCTGTGT+CGG + chr8.3:18910363-18910382 None:intergenic 50.0%
CCCAGAATTCCCATACCTGA+CGG + chr8.3:18910510-18910529 None:intergenic 50.0%
CCGTCAGGTATGGGAATTCT+GGG - chr8.3:18910507-18910526 MS.gene72999:CDS 50.0%
CGAATTCTCTCACGATGTCG+AGG + chr8.3:18910319-18910338 None:intergenic 50.0%
GGAGTCTTCGATAATTGGGC+TGG + chr8.3:18910192-18910211 None:intergenic 50.0%
GTAAAACCCGCCACCAAACT+TGG + chr8.3:18910488-18910507 None:intergenic 50.0%
TGTGTGTGGTTGGAAGCTAG+AGG + chr8.3:18910249-18910268 None:intergenic 50.0%
TTGCTTCGATGAGGAGGTTG+AGG + chr8.3:18910280-18910299 None:intergenic 50.0%
! CGGGTTTTACCGTCAGGTAT+GGG - chr8.3:18910498-18910517 MS.gene72999:CDS 50.0%
! CTTGATCCAAGTTTGGTGGC+GGG - chr8.3:18910479-18910498 MS.gene72999:CDS 50.0%
! GATTTTGTCGATGTCAGCGG+TGG + chr8.3:18910129-18910148 None:intergenic 50.0%
!! AACCGACACAGAGCTTGTTG+TGG - chr8.3:18910358-18910377 MS.gene72999:CDS 50.0%
!! GCTTGTTGTGGTGTCTTCAG+TGG - chr8.3:18910370-18910389 MS.gene72999:CDS 50.0%
!! GTCGAGGGTGTTGTTGGATT+TGG + chr8.3:18910102-18910121 None:intergenic 50.0%
GGCTAAATCGGCTAATGCGG+TGG + chr8.3:18910081-18910100 None:intergenic 55.0%
TCACAGGGGCGAAGAATGTG+AGG - chr8.3:18910444-18910463 MS.gene72999:CDS 55.0%
TTTGTCGATGTCAGCGGTGG+TGG + chr8.3:18910126-18910145 None:intergenic 55.0%
! GCGGGTTTTACCGTCAGGTA+TGG - chr8.3:18910497-18910516 MS.gene72999:CDS 55.0%
TAATGCGGTGGCGTAGCTGG+CGG + chr8.3:18910069-18910088 None:intergenic 60.0%
!!! TGGTGGCGGGTTTTACCGTC+AGG - chr8.3:18910492-18910511 MS.gene72999:CDS 60.0%
GGCTAATGCGGTGGCGTAGC+TGG + chr8.3:18910072-18910091 None:intergenic 65.0%
!! GGTGGCGTCGAGGGTGTTGT+TGG + chr8.3:18910108-18910127 None:intergenic 65.0%
! GTCAGCGGTGGTGGCGTCGA+GGG + chr8.3:18910117-18910136 None:intergenic 70.0%
! TGTCAGCGGTGGTGGCGTCG+AGG + chr8.3:18910118-18910137 None:intergenic 70.0%
TGCGGTGGCGTAGCTGGCGG+CGG + chr8.3:18910066-18910085 None:intergenic 75.0%
TGGCGTAGCTGGCGGCGGCT+GGG + chr8.3:18910061-18910080 None:intergenic 75.0%
! GGCGTAGCTGGCGGCGGCTG+GGG + chr8.3:18910060-18910079 None:intergenic 80.0%
! GTGGCGTAGCTGGCGGCGGC+TGG + chr8.3:18910062-18910081 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 18910050 18910616 18910050 ID=MS.gene72999
chr8.3 mRNA 18910050 18910616 18910050 ID=MS.gene72999.t1;Parent=MS.gene72999
chr8.3 exon 18910050 18910616 18910050 ID=MS.gene72999.t1.exon1;Parent=MS.gene72999.t1
chr8.3 CDS 18910050 18910616 18910050 ID=cds.MS.gene72999.t1;Parent=MS.gene72999.t1
Gene Sequence

>MS.gene72999

ATGAACTCCCCAGCCGCCGCCAGCTACGCCACCGCATTAGCCGATTTAGCCAAATCCAACAACACCCTCGACGCCACCACCGCTGACATCGACAAAATCGAAGAACTCTTCACTGACCCCAAGGTGTTCGACTATTTCTCCAGCCCAATTATCGAAGACTCCACCAAACGCAAACTCATCGCTGAATTCGCTACTTCCTCTAGCTTCCAACCACACACACAAAACTTCCTCAACCTCCTCATCGAAGCAAACAGAATCGACATCATCCTCGACATCGTGAGAGAATTCGAACTTCATTACAATACTCTAACCGACACAGAGCTTGTTGTGGTGTCTTCAGTGGTGAAATTGGAGTCACAGCATTTAGCGCAGATTGCGAAACAGGTTCAAAAACTCACAGGGGCGAAGAATGTGAGGATTAAAACTGCTCTTGATCCAAGTTTGGTGGCGGGTTTTACCGTCAGGTATGGGAATTCTGGGTCTAAGTTTATTGATATGAGTGTTAAGAAGAAACTTGAGGAAATTGCTGCTCAACTTGATTTGGGTGATATTAAACTTGCTGCATGA

Protein sequence

>MS.gene72999.t1

MNSPAAASYATALADLAKSNNTLDATTADIDKIEELFTDPKVFDYFSSPIIEDSTKRKLIAEFATSSSFQPHTQNFLNLLIEANRIDIILDIVREFELHYNTLTDTELVVVSSVVKLESQHLAQIAKQVQKLTGAKNVRIKTALDPSLVAGFTVRYGNSGSKFIDMSVKKKLEEIAAQLDLGDIKLAA