AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06408


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06408.t1 MTR_3g096290 89.091 165 12 1 1 165 1 159 5.27e-100 285
MS.gene06408.t1 MTR_3g096290 85.366 123 12 1 1 123 9 125 2.74e-65 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06408.t1 AT1G74880 58.788 165 61 1 1 165 1 158 1.60e-62 190

Find 45 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCTTAGGAAGAGTCTT+AGG 0.205905 3.2:+80399601 None:intergenic
GTAGTCTAATCCTTTGTAAT+AGG 0.224129 3.2:+80399297 None:intergenic
CTGGAATAAACAGGTTTCTT+AGG 0.242562 3.2:+80399589 None:intergenic
CTTGACTCACTGGAATAAAC+AGG 0.396415 3.2:+80399580 None:intergenic
GATATGCGTATATTTGAAAC+AGG 0.408664 3.2:-80399157 MS.gene06408:CDS
TCTTGAACTGCACGAATCAA+TGG 0.413571 3.2:+80399715 None:intergenic
GTTTCTTAGGAAGAGTCTTA+GGG 0.440236 3.2:+80399602 None:intergenic
TGCTTAATTCTCAGGTTGCA+TGG 0.442545 3.2:-80398944 MS.gene06408:intron
GCAGTTCAAGACGGTGAACC+AGG 0.447486 3.2:-80399703 MS.gene06408:CDS
TAGTCTAATCCTTTGTAATA+GGG 0.459657 3.2:+80399298 None:intergenic
GCATCAGAGCTCTTAGTTTG+TGG 0.477417 3.2:+80399667 None:intergenic
TTTGAGAAATGGGAAAGAGA+AGG 0.496635 3.2:+80399772 None:intergenic
CTTGAACTGCACGAATCAAT+GGG 0.503362 3.2:+80399716 None:intergenic
GGAAGCCAAGCTGGAGCAGT+GGG 0.508111 3.2:+80398912 None:intergenic
AGGAAGAGTCTTAGGGGCAG+TGG 0.518025 3.2:+80399609 None:intergenic
ATGGGAAAGAGAAGGAAAGG+AGG 0.524817 3.2:+80399780 None:intergenic
GAAATGGGAAAGAGAAGGAA+AGG 0.530112 3.2:+80399777 None:intergenic
GCTTAATTCTCAGGTTGCAT+GGG 0.543172 3.2:-80398943 MS.gene06408:intron
TCCTACATACATGCTTATCA+AGG 0.544432 3.2:-80398892 MS.gene06408:intron
AGGAAGCCAAGCTGGAGCAG+TGG 0.544685 3.2:+80398911 None:intergenic
GAGTCCCCACTGCTCCAGCT+TGG 0.545534 3.2:-80398917 MS.gene06408:CDS
AATTTGTGGACAGTGAAGAA+AGG 0.549691 3.2:-80399480 MS.gene06408:intron
AATTCTCAGGTTGCATGGGT+AGG 0.550094 3.2:-80398939 MS.gene06408:intron
TCTAATCCTTTGTAATAGGG+TGG 0.556199 3.2:+80399301 None:intergenic
TGAAGAAAGGTCAGATTGTG+AGG 0.558487 3.2:-80399467 MS.gene06408:CDS
ATGTATGTAGGAAGCCAAGC+TGG 0.563654 3.2:+80398903 None:intergenic
GGGCACCCACCCTATTACAA+AGG 0.572065 3.2:-80399307 MS.gene06408:CDS
TCAGATTGTGAGGGTGGACA+AGG 0.576125 3.2:-80399457 MS.gene06408:CDS
GAAAGGTCAGATTGTGAGGG+TGG 0.585375 3.2:-80399463 MS.gene06408:CDS
CTAATCCTTTGTAATAGGGT+GGG 0.595783 3.2:+80399302 None:intergenic
CAGTTCAAGACGGTGAACCA+GGG 0.597426 3.2:-80399702 MS.gene06408:CDS
AGCACTACTAGAGGAGGCTG+AGG 0.602396 3.2:+80399642 None:intergenic
TTGATTCGTGCAGTTCAAGA+CGG 0.616062 3.2:-80399712 MS.gene06408:CDS
TTTGTTTGTTTACCTCACCA+CGG 0.617430 3.2:+80399263 None:intergenic
AGTTCAAGACGGTGAACCAG+GGG 0.628235 3.2:-80399701 MS.gene06408:CDS
ACCTTGATAAGCATGTATGT+AGG 0.632174 3.2:+80398891 None:intergenic
GAATCAATGGGAAAGAAGAA+CGG 0.633893 3.2:+80399728 None:intergenic
TTTCTTAGGAAGAGTCTTAG+GGG 0.641863 3.2:+80399603 None:intergenic
GAAGAAAGGTCAGATTGTGA+GGG 0.659835 3.2:-80399466 MS.gene06408:CDS
AGACTGAGCACTACTAGAGG+AGG 0.683341 3.2:+80399636 None:intergenic
GACTACATTTACGAAGACCG+TGG 0.695548 3.2:-80399280 MS.gene06408:CDS
TGAAGACTGAGCACTACTAG+AGG 0.698555 3.2:+80399633 None:intergenic
CATTTACGAAGACCGTGGTG+AGG 0.713150 3.2:-80399275 MS.gene06408:intron
GTTCAAGACGGTGAACCAGG+GGG 0.724086 3.2:-80399700 MS.gene06408:CDS
GAAGCCAAGCTGGAGCAGTG+GGG 0.758823 3.2:+80398913 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTGTGTAAAAAAATTAT+TGG + chr3.2:80399472-80399491 None:intergenic 15.0%
!!! ATTTTGTTTTCATAATCTTT+TGG + chr3.2:80399294-80399313 None:intergenic 15.0%
!!! TATTCATTTTTGTTAATTTG+TGG - chr3.2:80399035-80399054 MS.gene06408:intron 15.0%
! AAATGTCAAACTGTATTTGT+AGG - chr3.2:80399344-80399363 MS.gene06408:intron 25.0%
! AGATACTGAAAAATTAACCT+TGG + chr3.2:80399195-80399214 None:intergenic 25.0%
! ATTTCTCAAAAACTTTGACA+AGG - chr3.2:80398770-80398789 MS.gene06408:intron 25.0%
! TAGTCTAATCCTTTGTAATA+GGG + chr3.2:80399234-80399253 None:intergenic 25.0%
!! GTTTTCTATGATTCCAATTA+TGG - chr3.2:80399504-80399523 MS.gene06408:intron 25.0%
!!! ATTTTTCAGTATCTTTCTGT+TGG - chr3.2:80399201-80399220 MS.gene06408:intron 25.0%
!!! TGTCAAAGTTTTTGAGAAAT+GGG + chr3.2:80398770-80398789 None:intergenic 25.0%
!!! TTGTCAAAGTTTTTGAGAAA+TGG + chr3.2:80398771-80398790 None:intergenic 25.0%
!!! TTTTTCAGTATCTTTCTGTT+GGG - chr3.2:80399202-80399221 MS.gene06408:intron 25.0%
CAACAAAAAAAGTAAGAGTG+AGG + chr3.2:80399781-80399800 None:intergenic 30.0%
CTTCACAATGTTACCATAAT+TGG + chr3.2:80399520-80399539 None:intergenic 30.0%
GTAGTCTAATCCTTTGTAAT+AGG + chr3.2:80399235-80399254 None:intergenic 30.0%
TAATGCTTTACTTGTTCCAA+TGG - chr3.2:80399660-80399679 MS.gene06408:CDS 30.0%
! GATATGCGTATATTTGAAAC+AGG - chr3.2:80399372-80399391 MS.gene06408:intron 30.0%
AATTTGTGGACAGTGAAGAA+AGG - chr3.2:80399049-80399068 MS.gene06408:intron 35.0%
ACCTTGATAAGCATGTATGT+AGG + chr3.2:80399641-80399660 None:intergenic 35.0%
CAAACTGTATTTGTAGGTCT+TGG - chr3.2:80399350-80399369 MS.gene06408:intron 35.0%
CTAATCCTTTGTAATAGGGT+GGG + chr3.2:80399230-80399249 None:intergenic 35.0%
CTGGAATAAACAGGTTTCTT+AGG + chr3.2:80398943-80398962 None:intergenic 35.0%
GAATCAATGGGAAAGAAGAA+CGG + chr3.2:80398804-80398823 None:intergenic 35.0%
GTTTCTTAGGAAGAGTCTTA+GGG + chr3.2:80398930-80398949 None:intergenic 35.0%
TATTAGATTGTAGCAGCCAT+TGG + chr3.2:80399679-80399698 None:intergenic 35.0%
TCCTACATACATGCTTATCA+AGG - chr3.2:80399637-80399656 MS.gene06408:CDS 35.0%
TCTAATCCTTTGTAATAGGG+TGG + chr3.2:80399231-80399250 None:intergenic 35.0%
TGTACCATTGCTTAATTCTC+AGG - chr3.2:80399577-80399596 MS.gene06408:intron 35.0%
TTTCTTAGGAAGAGTCTTAG+GGG + chr3.2:80398929-80398948 None:intergenic 35.0%
TTTGAGAAATGGGAAAGAGA+AGG + chr3.2:80398760-80398779 None:intergenic 35.0%
TTTGTTTGTTTACCTCACCA+CGG + chr3.2:80399269-80399288 None:intergenic 35.0%
CTTGAACTGCACGAATCAAT+GGG + chr3.2:80398816-80398835 None:intergenic 40.0%
CTTGACTCACTGGAATAAAC+AGG + chr3.2:80398952-80398971 None:intergenic 40.0%
GAAATGGGAAAGAGAAGGAA+AGG + chr3.2:80398755-80398774 None:intergenic 40.0%
GAAGAAAGGTCAGATTGTGA+GGG - chr3.2:80399063-80399082 MS.gene06408:intron 40.0%
GCAACCTGAGAATTAAGCAA+TGG + chr3.2:80399584-80399603 None:intergenic 40.0%
GGACTCAAATAACATGTCCA+AGG - chr3.2:80399175-80399194 MS.gene06408:intron 40.0%
GGTTTCTTAGGAAGAGTCTT+AGG + chr3.2:80398931-80398950 None:intergenic 40.0%
TAGTTATGTCAGCTGTGAGT+AGG - chr3.2:80399151-80399170 MS.gene06408:CDS 40.0%
TATTTGGTTGCTTGACTCAC+TGG + chr3.2:80398962-80398981 None:intergenic 40.0%
TCTTGAACTGCACGAATCAA+TGG + chr3.2:80398817-80398836 None:intergenic 40.0%
TGAAGAAAGGTCAGATTGTG+AGG - chr3.2:80399062-80399081 MS.gene06408:intron 40.0%
! GCTTAATTCTCAGGTTGCAT+GGG - chr3.2:80399586-80399605 MS.gene06408:intron 40.0%
! TGCTTAATTCTCAGGTTGCA+TGG - chr3.2:80399585-80399604 MS.gene06408:intron 40.0%
! TTGATTCGTGCAGTTCAAGA+CGG - chr3.2:80398817-80398836 MS.gene06408:intron 40.0%
ATGGGAAAGAGAAGGAAAGG+AGG + chr3.2:80398752-80398771 None:intergenic 45.0%
ATGTATGTAGGAAGCCAAGC+TGG + chr3.2:80399629-80399648 None:intergenic 45.0%
GACTACATTTACGAAGACCG+TGG - chr3.2:80399249-80399268 MS.gene06408:intron 45.0%
TGAAGACTGAGCACTACTAG+AGG + chr3.2:80398899-80398918 None:intergenic 45.0%
TTATGTCAGCTGTGAGTAGG+AGG - chr3.2:80399154-80399173 MS.gene06408:CDS 45.0%
! AATTCTCAGGTTGCATGGGT+AGG - chr3.2:80399590-80399609 MS.gene06408:CDS 45.0%
! GCATCAGAGCTCTTAGTTTG+TGG + chr3.2:80398865-80398884 None:intergenic 45.0%
!! AATTCAAATAAATATTTATT+TGG + chr3.2:80398978-80398997 None:intergenic 5.0%
AGACTGAGCACTACTAGAGG+AGG + chr3.2:80398896-80398915 None:intergenic 50.0%
AGTTCAAGACGGTGAACCAG+GGG - chr3.2:80398828-80398847 MS.gene06408:intron 50.0%
CAGTTCAAGACGGTGAACCA+GGG - chr3.2:80398827-80398846 MS.gene06408:intron 50.0%
CATTTACGAAGACCGTGGTG+AGG - chr3.2:80399254-80399273 MS.gene06408:intron 50.0%
GAAAGGTCAGATTGTGAGGG+TGG - chr3.2:80399066-80399085 MS.gene06408:intron 50.0%
GTCAGTAGTGTGTGTGCTGT+AGG - chr3.2:80399419-80399438 MS.gene06408:intron 50.0%
GTGCTGTAGGTGTGAACCAA+AGG - chr3.2:80399432-80399451 MS.gene06408:intron 50.0%
TCAGATTGTGAGGGTGGACA+AGG - chr3.2:80399072-80399091 MS.gene06408:intron 50.0%
AGCACTACTAGAGGAGGCTG+AGG + chr3.2:80398890-80398909 None:intergenic 55.0%
AGGAAGAGTCTTAGGGGCAG+TGG + chr3.2:80398923-80398942 None:intergenic 55.0%
GCAGTTCAAGACGGTGAACC+AGG - chr3.2:80398826-80398845 MS.gene06408:intron 55.0%
GGGCACCCACCCTATTACAA+AGG - chr3.2:80399222-80399241 MS.gene06408:intron 55.0%
GTTCAAGACGGTGAACCAGG+GGG - chr3.2:80398829-80398848 MS.gene06408:intron 55.0%
AGGAAGCCAAGCTGGAGCAG+TGG + chr3.2:80399621-80399640 None:intergenic 60.0%
GAAGCCAAGCTGGAGCAGTG+GGG + chr3.2:80399619-80399638 None:intergenic 60.0%
GGAAGCCAAGCTGGAGCAGT+GGG + chr3.2:80399620-80399639 None:intergenic 60.0%
GGCATGCTGTCAGCTGCCTT+TGG + chr3.2:80399451-80399470 None:intergenic 60.0%
GAGTCCCCACTGCTCCAGCT+TGG - chr3.2:80399612-80399631 MS.gene06408:CDS 65.0%
TGTGGCTGTGTACTGCCCCC+TGG + chr3.2:80398847-80398866 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 80398718 80399833 80398718 ID=MS.gene06408
chr3.2 mRNA 80398718 80399833 80398718 ID=MS.gene06408.t1;Parent=MS.gene06408
chr3.2 exon 80399590 80399833 80399590 ID=MS.gene06408.t1.exon1;Parent=MS.gene06408.t1
chr3.2 CDS 80399590 80399833 80399590 ID=cds.MS.gene06408.t1;Parent=MS.gene06408.t1
chr3.2 exon 80399434 80399489 80399434 ID=MS.gene06408.t1.exon2;Parent=MS.gene06408.t1
chr3.2 CDS 80399434 80399489 80399434 ID=cds.MS.gene06408.t1;Parent=MS.gene06408.t1
chr3.2 exon 80399276 80399341 80399276 ID=MS.gene06408.t1.exon3;Parent=MS.gene06408.t1
chr3.2 CDS 80399276 80399341 80399276 ID=cds.MS.gene06408.t1;Parent=MS.gene06408.t1
chr3.2 exon 80399144 80399185 80399144 ID=MS.gene06408.t1.exon4;Parent=MS.gene06408.t1
chr3.2 CDS 80399144 80399185 80399144 ID=cds.MS.gene06408.t1;Parent=MS.gene06408.t1
chr3.2 exon 80398893 80398952 80398893 ID=MS.gene06408.t1.exon5;Parent=MS.gene06408.t1
chr3.2 CDS 80398893 80398952 80398893 ID=cds.MS.gene06408.t1;Parent=MS.gene06408.t1
chr3.2 exon 80398718 80398747 80398718 ID=MS.gene06408.t1.exon6;Parent=MS.gene06408.t1
chr3.2 CDS 80398718 80398747 80398718 ID=cds.MS.gene06408.t1;Parent=MS.gene06408.t1
Gene Sequence

>MS.gene06408

ATGGCTTTCTGTGTAACTCTCTGCTACTACACCTCCTTTCCTTCTCTTTCCCATTTCTCAAAAACTTTGACAAGGAAACATGTCCGTTCTTCTTTCCCATTGATTCGTGCAGTTCAAGACGGTGAACCAGGGGGCAGTACACAGCCACAAACTAAGAGCTCTGATGCTTCCTCAGCCTCCTCTAGTAGTGCTCAGTCTTCAACCACTGCCCCTAAGACTCTTCCTAAGAAACCTGTTTATTCCAGTGAGTCAAGCAACCAAATAAATATTTATTTGAATTTTATATATATACTGAATGCTGCTGCTGTCTGTGTGAGTATTCATTTTTGTTAATTTGTGGACAGTGAAGAAAGGTCAGATTGTGAGGGTGGACAAGGAGAAATATCTCAATAGCATCAATGTAAGAAGACTATTATTGATTTAGTTTCAATTATAGTTATGTCAGCTGTGAGTAGGAGGACTCAAATAACATGTCCAAGGTTAATTTTTCAGTATCTTTCTGTTGGGCACCCACCCTATTACAAAGGATTAGACTACATTTACGAAGACCGTGGTGAGGTAAACAAACAAACACCAAAAGATTATGAAAACAAAATACTTTATATGATTAGTGTTTATCTAACTAGAAATGTCAAACTGTATTTGTAGGTCTTGGATATGCGTATATTTGAAACAGGAGAGCATGCACTTGTAAGTAACTTGTCAGTAGTGTGTGTGCTGTAGGTGTGAACCAAAGGCAGCTGACAGCATGCCAATAATTTTTTTACACAAACAACATGACTATTTGTTTTCTATGATTCCAATTATGGTAACATTGTGAAGTATGTGAAGTTTAAGTTTTATTATTGTGACACAAAACTGTACCATTGCTTAATTCTCAGGTTGCATGGGTAGGAGTCCCCACTGCTCCAGCTTGGCTTCCTACATACATGCTTATCAAGGTAATGCTTTACTTGTTCCAATGGCTGCTACAATCTAATAGTAAATGAAATCTGATGTAAATTGTTTATTTTTATCTACATTTACATGACTTCTTTACATTTTAGTTGCTTTGAAAGCACCTCACTCTTACTTTTTTTGTTGCAGTCAGAGAAGCTCAATTATGAGAGACTATAA

Protein sequence

>MS.gene06408.t1

MAFCVTLCYYTSFPSLSHFSKTLTRKHVRSSFPLIRAVQDGEPGGSTQPQTKSSDASSASSSSAQSSTTAPKTLPKKPVYSMKKGQIVRVDKEKYLNSINYLSVGHPPYYKGLDYIYEDRGEVLDMRIFETGEHALVAWVGVPTAPAWLPTYMLIKSEKLNYERL