AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80239


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80239.t1 MTR_3g106820 98.780 164 2 0 1 164 46 209 4.24e-113 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80239.t1 AT4G32260 78.431 153 31 1 11 161 66 218 6.95e-70 211

Find 36 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGAAGCTCTTGCAAATT+TGG 0.138611 3.4:-93049575 MS.gene80239:CDS
TTCCAACTCCTTCACTTCTT+CGG 0.354892 3.4:+93049729 None:intergenic
AGAAATTCAACGACTATGAT+TGG 0.371223 3.4:+93049859 None:intergenic
CACCCCTCTCGGAACCTTCA+TGG 0.382299 3.4:-93049803 MS.gene80239:CDS
GAACCTTGTTAACAATATCT+TGG 0.384952 3.4:+93049497 None:intergenic
TTGAGATATCAACGAGAAAC+AGG 0.396683 3.4:+93049472 None:intergenic
TGCAAATTTGGAAAAGCAAA+AGG 0.407369 3.4:-93049563 MS.gene80239:CDS
CGCAGCTCTCAAAACTGCAT+TGG 0.476740 3.4:+93049699 None:intergenic
GAAATTCAACGACTATGATT+GGG 0.478683 3.4:+93049860 None:intergenic
CGCGTCACGATTGTCCATGA+AGG 0.481050 3.4:+93049789 None:intergenic
CTTCACTTCTTCGGATGTGT+TGG 0.483273 3.4:+93049738 None:intergenic
GGAAGTTGCCGCTAGGGTCA+TGG 0.514941 3.4:+93049954 None:intergenic
AAACTCTACTTCACCCCTCT+CGG 0.522559 3.4:-93049814 MS.gene80239:CDS
AGAGAGGGAAGTTGCCGCTA+GGG 0.535819 3.4:+93049948 None:intergenic
AAGAGAGGGAAGTTGCCGCT+AGG 0.555867 3.4:+93049947 None:intergenic
TAGCCAAGATATTGTTAACA+AGG 0.566254 3.4:-93049500 MS.gene80239:CDS
AGCGAGGGAAGGTGGTGCGA+AGG 0.566516 3.4:+93049921 None:intergenic
ATCCGAAGAAGTGAAGGAGT+TGG 0.580997 3.4:-93049731 MS.gene80239:CDS
TGCGCGTGCTGAAATTGCGG+TGG 0.591536 3.4:-93049680 MS.gene80239:CDS
AAGCGAAGATTGCGGAAGGT+AGG 0.592242 3.4:-93049618 MS.gene80239:CDS
TGGTGCGAAGGCGAAAGAGA+GGG 0.595311 3.4:+93049933 None:intergenic
CGACTATGATTGGGAGAGTG+AGG 0.595912 3.4:+93049869 None:intergenic
CAACACATCCGAAGAAGTGA+AGG 0.596455 3.4:-93049737 MS.gene80239:CDS
AGCTGCGCGTGCTGAAATTG+CGG 0.598150 3.4:-93049683 MS.gene80239:CDS
GTTTGTCGAGAGCGAACATG+AGG 0.598866 3.4:+93049833 None:intergenic
GTGGTGCGAAGGCGAAAGAG+AGG 0.606516 3.4:+93049932 None:intergenic
GTCCATGAAGGTTCCGAGAG+GGG 0.611279 3.4:+93049801 None:intergenic
TGTCCATGAAGGTTCCGAGA+GGG 0.615991 3.4:+93049800 None:intergenic
AATCGTGACGCGGAAATCAG+AGG 0.616507 3.4:-93049778 MS.gene80239:CDS
GATTGCGGAAGGTAGGAAGA+AGG 0.621119 3.4:-93049611 MS.gene80239:CDS
GTTGAAGCGAAGATTGCGGA+AGG 0.664257 3.4:-93049622 MS.gene80239:CDS
GAGGTTGAAATCGAAGAGTG+CGG 0.686862 3.4:+93049888 None:intergenic
TGAAGTTGAAGCGAAGATTG+CGG 0.692066 3.4:-93049626 MS.gene80239:CDS
ACTCAAATCCATGACCCTAG+CGG 0.699556 3.4:-93049962 None:intergenic
CTTCATGGACAATCGTGACG+CGG 0.701097 3.4:-93049788 MS.gene80239:CDS
TTGTCCATGAAGGTTCCGAG+AGG 0.735316 3.4:+93049799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATTCAACGACTATGAT+TGG + chr3.4:93049576-93049595 None:intergenic 30.0%
GAAATTCAACGACTATGATT+GGG + chr3.4:93049575-93049594 None:intergenic 30.0%
GAACCTTGTTAACAATATCT+TGG + chr3.4:93049938-93049957 None:intergenic 30.0%
TAGCCAAGATATTGTTAACA+AGG - chr3.4:93049932-93049951 MS.gene80239:CDS 30.0%
TGCAAATTTGGAAAAGCAAA+AGG - chr3.4:93049869-93049888 MS.gene80239:CDS 30.0%
GCAAGAAGCTCTTGCAAATT+TGG - chr3.4:93049857-93049876 MS.gene80239:CDS 40.0%
TGAAGTTGAAGCGAAGATTG+CGG - chr3.4:93049806-93049825 MS.gene80239:CDS 40.0%
TTCCAACTCCTTCACTTCTT+CGG + chr3.4:93049706-93049725 None:intergenic 40.0%
AAACTCTACTTCACCCCTCT+CGG - chr3.4:93049618-93049637 MS.gene80239:CDS 45.0%
ATCCGAAGAAGTGAAGGAGT+TGG - chr3.4:93049701-93049720 MS.gene80239:CDS 45.0%
CAACACATCCGAAGAAGTGA+AGG - chr3.4:93049695-93049714 MS.gene80239:CDS 45.0%
CTTCACTTCTTCGGATGTGT+TGG + chr3.4:93049697-93049716 None:intergenic 45.0%
GAGGTTGAAATCGAAGAGTG+CGG + chr3.4:93049547-93049566 None:intergenic 45.0%
AAGCGAAGATTGCGGAAGGT+AGG - chr3.4:93049814-93049833 MS.gene80239:CDS 50.0%
AATCGTGACGCGGAAATCAG+AGG - chr3.4:93049654-93049673 MS.gene80239:CDS 50.0%
CGACTATGATTGGGAGAGTG+AGG + chr3.4:93049566-93049585 None:intergenic 50.0%
CGCAGCTCTCAAAACTGCAT+TGG + chr3.4:93049736-93049755 None:intergenic 50.0%
CTTCATGGACAATCGTGACG+CGG - chr3.4:93049644-93049663 MS.gene80239:CDS 50.0%
GATTGCGGAAGGTAGGAAGA+AGG - chr3.4:93049821-93049840 MS.gene80239:CDS 50.0%
GTTGAAGCGAAGATTGCGGA+AGG - chr3.4:93049810-93049829 MS.gene80239:CDS 50.0%
GTTTGTCGAGAGCGAACATG+AGG + chr3.4:93049602-93049621 None:intergenic 50.0%
TGTCCATGAAGGTTCCGAGA+GGG + chr3.4:93049635-93049654 None:intergenic 50.0%
TTGTCCATGAAGGTTCCGAG+AGG + chr3.4:93049636-93049655 None:intergenic 50.0%
! TTTTCGAAAGCGAGGGAAGG+TGG + chr3.4:93049522-93049541 None:intergenic 50.0%
!!! GCTTTTTCGAAAGCGAGGGA+AGG + chr3.4:93049525-93049544 None:intergenic 50.0%
!!! TGCGGCTTTTTCGAAAGCGA+GGG + chr3.4:93049529-93049548 None:intergenic 50.0%
AAGAGAGGGAAGTTGCCGCT+AGG + chr3.4:93049488-93049507 None:intergenic 55.0%
AGAGAGGGAAGTTGCCGCTA+GGG + chr3.4:93049487-93049506 None:intergenic 55.0%
AGCTGCGCGTGCTGAAATTG+CGG - chr3.4:93049749-93049768 MS.gene80239:CDS 55.0%
CGCGTCACGATTGTCCATGA+AGG + chr3.4:93049646-93049665 None:intergenic 55.0%
GTCCATGAAGGTTCCGAGAG+GGG + chr3.4:93049634-93049653 None:intergenic 55.0%
TGGTGCGAAGGCGAAAGAGA+GGG + chr3.4:93049502-93049521 None:intergenic 55.0%
!!! GTGCGGCTTTTTCGAAAGCG+AGG + chr3.4:93049530-93049549 None:intergenic 55.0%
CACCCCTCTCGGAACCTTCA+TGG - chr3.4:93049629-93049648 MS.gene80239:CDS 60.0%
GTGGTGCGAAGGCGAAAGAG+AGG + chr3.4:93049503-93049522 None:intergenic 60.0%
TGCGCGTGCTGAAATTGCGG+TGG - chr3.4:93049752-93049771 MS.gene80239:CDS 60.0%
AGCGAGGGAAGGTGGTGCGA+AGG + chr3.4:93049514-93049533 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 93049480 93049974 93049480 ID=MS.gene80239
chr3.4 mRNA 93049480 93049974 93049480 ID=MS.gene80239.t1;Parent=MS.gene80239
chr3.4 exon 93049480 93049974 93049480 ID=MS.gene80239.t1.exon1;Parent=MS.gene80239.t1
chr3.4 CDS 93049480 93049974 93049480 ID=cds.MS.gene80239.t1;Parent=MS.gene80239.t1
Gene Sequence

>MS.gene80239

ATGACCCTAGCGGCAACTTCCCTCTCTTTCGCCTTCGCACCACCTTCCCTCGCTTTCGAAAAAGCCGCACTCTTCGATTTCAACCTCACTCTCCCAATCATAGTCGTTGAATTTCTCTTCCTCATGTTCGCTCTCGACAAACTCTACTTCACCCCTCTCGGAACCTTCATGGACAATCGTGACGCGGAAATCAGAGGCAAACTCAACAGCGTCACCAACACATCCGAAGAAGTGAAGGAGTTGGAAGAACAAGCCAATGCAGTTTTGAGAGCTGCGCGTGCTGAAATTGCGGTGGCGCTGAATCAGATGAAGAAAGAGACTCAAGCTGAAGTTGAAGCGAAGATTGCGGAAGGTAGGAAGAAGGTTGATGCTGAATTGCAAGAAGCTCTTGCAAATTTGGAAAAGCAAAAGGAAGAAACTGTTAAAGCTCTTGATTCTCAGATTGCTGCTCTTAGCCAAGATATTGTTAACAAGGTTCTTCCTGTTTCTCGTTGA

Protein sequence

>MS.gene80239.t1

MTLAATSLSFAFAPPSLAFEKAALFDFNLTLPIIVVEFLFLMFALDKLYFTPLGTFMDNRDAEIRGKLNSVTNTSEEVKELEEQANAVLRAARAEIAVALNQMKKETQAEVEAKIAEGRKKVDAELQEALANLEKQKEETVKALDSQIAALSQDIVNKVLPVSR