AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44068

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44068.t1 MTR_1g082420 98.962 289 3 0 1 289 1 289 0.0 578
MS.gene44068.t1 MTR_1g082420 98.551 207 3 0 1 207 1 207 3.03e-147 411
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44068.t1 AT1G54780 69.595 296 72 3 1 289 1 285 5.44e-138 391

Find 68 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGAGTTGGGAGGAAAA+AGG 0.232772 1.3:+58027160 None:intergenic
GAAACGGTTGTTATCTGATT+TGG 0.264813 1.3:-58026812 MS.gene44068:CDS
GCATTACCACTAAGTAAAAC+TGG 0.283086 1.3:+58026934 None:intergenic
TGAGTTTGCGGACCAAATTT+TGG 0.287868 1.3:-58026164 MS.gene44068:CDS
GATACCAACGCTCCAAAATT+TGG 0.302536 1.3:+58026152 None:intergenic
GAAATGAGAGAGGTTGATTT+GGG 0.321070 1.3:+58027099 None:intergenic
GGAAATGAGAGAGGTTGATT+TGG 0.327476 1.3:+58027098 None:intergenic
CTTACTCACTCATGCTCAAC+AGG 0.332202 1.3:-58026998 MS.gene44068:CDS
ATTAGAAACAATGGGTTTGT+AGG 0.342048 1.3:+58027077 None:intergenic
TCTTGTAGTTGGAGGTTTGT+TGG 0.348630 1.3:-58025047 MS.gene44068:CDS
ACCGTTTCAGAGAACCTTCC+AGG 0.350686 1.3:-58026010 MS.gene44068:intron
TCAACCACATAGGCCTCTTT+AGG 0.375166 1.3:+58026874 None:intergenic
AGAAGTGGAGAGAGAGAGTT+GGG 0.377846 1.3:+58027150 None:intergenic
CAGAAAGAAGGAGCAGTTAC+TGG 0.391425 1.3:-58026079 MS.gene44068:CDS
GGAGAGAAGGTGAAGATTTG+AGG 0.395997 1.3:+58027124 None:intergenic
TGAGAGAGGTTGATTTGGGT+TGG 0.410728 1.3:+58027103 None:intergenic
CAATTCTCTCTTGTAGTTGG+AGG 0.420228 1.3:-58025055 MS.gene44068:CDS
GTGCATGAAGGAAGGGTTGG+TGG 0.423912 1.3:+58027024 None:intergenic
TATTGCATTTGTTGTTCCTA+TGG 0.427254 1.3:-58025023 MS.gene44068:CDS
CGACTAGTTGCTGCCATTGA+CGG 0.431818 1.3:-58025166 MS.gene44068:CDS
GTTGATTTGGGTTGGAGAGA+AGG 0.438011 1.3:+58027111 None:intergenic
ACCGAGGGACCACCAGGATC+TGG 0.448423 1.3:+58025139 None:intergenic
GGACAATTCTCTCTTGTAGT+TGG 0.450595 1.3:-58025058 MS.gene44068:CDS
GAGAAGTGGAGAGAGAGAGT+TGG 0.457937 1.3:+58027149 None:intergenic
GTGGAAGAAGGCAACGATAA+GGG 0.470857 1.3:-58026124 MS.gene44068:CDS
TGTGGAAGAAGGCAACGATA+AGG 0.488102 1.3:-58026125 MS.gene44068:CDS
ACGTTTGTCGTCTTTAACCG+AGG 0.492907 1.3:+58025123 None:intergenic
GCCTATGTGGTTGATGATGC+TGG 0.497501 1.3:-58026865 MS.gene44068:CDS
TTTACTTAGTGGTAATGCAT+TGG 0.498407 1.3:-58026929 MS.gene44068:CDS
TAATGAGCGTCCACCTAAAG+AGG 0.498637 1.3:-58026887 MS.gene44068:CDS
TCCAGATCCTGGTGGTCCCT+CGG 0.499224 1.3:-58025140 MS.gene44068:CDS
CAACCGTTTCAAATCAGACT+TGG 0.499905 1.3:+58026825 None:intergenic
ACCTGGAAGGTTCTCTGAAA+CGG 0.506810 1.3:+58026009 None:intergenic
GAAGATTTGAGGTTGAGAAG+TGG 0.512998 1.3:+58027135 None:intergenic
TTTGCATGCAGTATTGGCTA+CGG 0.514698 1.3:-58025227 MS.gene44068:intron
TGTTATCTGATTTGGAATCA+AGG 0.519003 1.3:-58026804 MS.gene44068:CDS
TCGTTGCCTTCTTCCACAGA+AGG 0.532690 1.3:+58026130 None:intergenic
TCTTTAACCGAGGGACCACC+AGG 0.532906 1.3:+58025133 None:intergenic
GTAAGGTTGTGCATGAAGGA+AGG 0.533369 1.3:+58027016 None:intergenic
CTGTTGAGCATGAGTGAGTA+AGG 0.534711 1.3:+58026999 None:intergenic
TGACCAAGTCTGATTTGAAA+CGG 0.535029 1.3:-58026828 MS.gene44068:CDS
ATTGACGGACTTCCAGATCC+TGG 0.535082 1.3:-58025151 MS.gene44068:CDS
AGGATCTGGAAGTCCGTCAA+TGG 0.539431 1.3:+58025153 None:intergenic
TAAGCATAGTATTGTACCAT+AGG 0.544493 1.3:+58025007 None:intergenic
TGGTATCCTTCTGTGGAAGA+AGG 0.548458 1.3:-58026136 MS.gene44068:CDS
CATTACCACTAAGTAAAACT+GGG 0.556805 1.3:+58026935 None:intergenic
CCTACGGCTTGAACAAAAGC+AGG 0.557352 1.3:+58026052 None:intergenic
GTTGTGCATGAAGGAAGGGT+TGG 0.563287 1.3:+58027021 None:intergenic
GGCAGCAACTAGTCGTTTAG+CGG 0.568536 1.3:+58025174 None:intergenic
CTTGTAACAAGTCAGAAAGA+AGG 0.568574 1.3:-58026091 MS.gene44068:CDS
GTGAGTAAGGTTGTGCATGA+AGG 0.585716 1.3:+58027012 None:intergenic
ACCACATAGGCCTCTTTAGG+TGG 0.587301 1.3:+58026877 None:intergenic
TATCACACTTAGAAAACTCA+CGG 0.590466 1.3:-58026761 MS.gene44068:intron
CGTTTGTCGTCTTTAACCGA+GGG 0.605535 1.3:+58025124 None:intergenic
GGAGCGTTGGTATCCTTCTG+TGG 0.606556 1.3:-58026143 MS.gene44068:CDS
GAAGAGACAGAAGAAAAGCG+TGG 0.608619 1.3:-58025079 MS.gene44068:CDS
GGGTTTGTAGGAAATGAGAG+AGG 0.610811 1.3:+58027089 None:intergenic
TCCACCTAAAGAGGCCTATG+TGG 0.614674 1.3:-58026878 MS.gene44068:CDS
AGTGGAGAGAGAGAGTTGGG+AGG 0.617964 1.3:+58027153 None:intergenic
TAAGGTTGTGCATGAAGGAA+GGG 0.619871 1.3:+58027017 None:intergenic
TGAGTCTAACTTCAAAACAA+AGG 0.628354 1.3:-58025101 MS.gene44068:CDS
AGGCAACGATAAGGGTGTCG+TGG 0.649353 1.3:-58026116 MS.gene44068:CDS
GCCATTAGGAAGTACCTGGA+AGG 0.650563 1.3:+58025996 None:intergenic
AAAGAAGGAGCAGTTACTGG+TGG 0.670414 1.3:-58026076 MS.gene44068:CDS
TCCAGCATCATCAACCACAT+AGG 0.674153 1.3:+58026864 None:intergenic
GACGGACTTCCAGATCCTGG+TGG 0.696278 1.3:-58025148 MS.gene44068:CDS
TTACTCACTCATGCTCAACA+GGG 0.696497 1.3:-58026997 MS.gene44068:CDS
TCTCTGAAACGGTAGCATCG+AGG 0.740488 1.3:+58026020 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTCATTGAATTAAATTA+GGG - chr1.3:58025438-58025457 MS.gene44068:intron 15.0%
!! TTATCTCATTGAATTAAATT+AGG - chr1.3:58025437-58025456 MS.gene44068:intron 15.0%
!! AATCATCATTGATATGAATA+TGG - chr1.3:58026382-58026401 MS.gene44068:intron 20.0%
!!! ACAACTTAATTAAGTTCTTT+TGG + chr1.3:58025646-58025665 None:intergenic 20.0%
!!! TTTTTCTTCACTAACAAATA+TGG - chr1.3:58026315-58026334 MS.gene44068:intron 20.0%
!!! TTTTTGAGATTAGAAACAAT+GGG + chr1.3:58025093-58025112 None:intergenic 20.0%
! AAACAAAAATGTTGAGCATT+TGG - chr1.3:58025930-58025949 MS.gene44068:intron 25.0%
! AATAAGCTAAAAACTGCAAA+TGG + chr1.3:58025553-58025572 None:intergenic 25.0%
! ATCATGATTATGTCTTTCAA+AGG - chr1.3:58026268-58026287 MS.gene44068:intron 25.0%
! CAACATACACTGAAATTAAA+TGG + chr1.3:58026737-58026756 None:intergenic 25.0%
! CATATCCATCATTATCTTTA+TGG + chr1.3:58026648-58026667 None:intergenic 25.0%
! CATATGTGTTGTATTGTAAT+GGG - chr1.3:58025836-58025855 MS.gene44068:intron 25.0%
! CTAAATAAAGGAGAAAACTT+CGG - chr1.3:58025681-58025700 MS.gene44068:intron 25.0%
! TACACTGAAATTAAATGGAT+AGG + chr1.3:58026732-58026751 None:intergenic 25.0%
! TCAAAACATAGCCTAAATAA+AGG - chr1.3:58025669-58025688 MS.gene44068:intron 25.0%
!! TCATCAATACACTGATTAAA+TGG - chr1.3:58025903-58025922 MS.gene44068:intron 25.0%
!! TTTTCTTGAAAACAAATGCA+AGG - chr1.3:58026468-58026487 MS.gene44068:intron 25.0%
!!! ATTGTTTTTCTGTAGCATAT+TGG - chr1.3:58026205-58026224 MS.gene44068:intron 25.0%
!!! CGGCACTTTTTTTTTTTTTT+TGG - chr1.3:58026540-58026559 MS.gene44068:intron 25.0%
!!! CTTTTTGAGATTAGAAACAA+TGG + chr1.3:58025094-58025113 None:intergenic 25.0%
!!! GTTTTTTGTGATAGCTAAAA+CGG + chr1.3:58026703-58026722 None:intergenic 25.0%
!!! TTGTTTTTCTGTAGCATATT+GGG - chr1.3:58026206-58026225 MS.gene44068:intron 25.0%
!!! TTTTTTGTGATAGCTAAAAC+GGG + chr1.3:58026702-58026721 None:intergenic 25.0%
!!! TTTTTTTTGGTTAAGAGTTG+AGG - chr1.3:58026553-58026572 MS.gene44068:intron 25.0%
AACGACCATAAAGATAATGA+TGG - chr1.3:58026640-58026659 MS.gene44068:intron 30.0%
AATTTCAGTGTATGTTGTCA+AGG - chr1.3:58026740-58026759 MS.gene44068:intron 30.0%
CATTACCACTAAGTAAAACT+GGG + chr1.3:58025227-58025246 None:intergenic 30.0%
CCATATGTGTTGTATTGTAA+TGG - chr1.3:58025835-58025854 MS.gene44068:intron 30.0%
CCATTACAATACAACACATA+TGG + chr1.3:58025838-58025857 None:intergenic 30.0%
TAAGCATAGTATTGTACCAT+AGG + chr1.3:58027155-58027174 None:intergenic 30.0%
TACATACTTCGGTTTAAACA+AGG - chr1.3:58026773-58026792 MS.gene44068:CDS 30.0%
TATCACACTTAGAAAACTCA+CGG - chr1.3:58025398-58025417 MS.gene44068:intron 30.0%
TGAGTCTAACTTCAAAACAA+AGG - chr1.3:58027058-58027077 MS.gene44068:CDS 30.0%
TTGTTTAAACCGAAGTATGT+AGG + chr1.3:58026774-58026793 None:intergenic 30.0%
TTTCAAAGGATTTACAAGAC+TGG - chr1.3:58026282-58026301 MS.gene44068:intron 30.0%
! ATTAGAAACAATGGGTTTGT+AGG + chr1.3:58025085-58025104 None:intergenic 30.0%
! TGTTTTTCTGTAGCATATTG+GGG - chr1.3:58026207-58026226 MS.gene44068:intron 30.0%
! TTTACTTAGTGGTAATGCAT+TGG - chr1.3:58025230-58025249 MS.gene44068:intron 30.0%
!! AGCAGTTGTGATCATTTTTA+GGG - chr1.3:58026885-58026904 MS.gene44068:CDS 30.0%
!! ATAAACACTCTGAGATTGTT+TGG - chr1.3:58025498-58025517 MS.gene44068:intron 30.0%
!! CGAAGTTTTCTCCTTTATTT+AGG + chr1.3:58025683-58025702 None:intergenic 30.0%
!! TAGCAGTTGTGATCATTTTT+AGG - chr1.3:58026884-58026903 MS.gene44068:CDS 30.0%
!! TATTGCATTTGTTGTTCCTA+TGG - chr1.3:58027136-58027155 MS.gene44068:CDS 30.0%
!! TGTTATCTGATTTGGAATCA+AGG - chr1.3:58025355-58025374 MS.gene44068:intron 30.0%
AGATTGTTTGGAAGAGCTTA+TGG - chr1.3:58025510-58025529 MS.gene44068:intron 35.0%
CATCTGTTCACAAACTATGA+AGG - chr1.3:58026359-58026378 MS.gene44068:intron 35.0%
CTAACAAATATGGCAAGTGT+TGG - chr1.3:58026325-58026344 MS.gene44068:intron 35.0%
CTTGTAACAAGTCAGAAAGA+AGG - chr1.3:58026068-58026087 MS.gene44068:CDS 35.0%
GAAACGGTTGTTATCTGATT+TGG - chr1.3:58025347-58025366 MS.gene44068:intron 35.0%
GCATTACCACTAAGTAAAAC+TGG + chr1.3:58025228-58025247 None:intergenic 35.0%
GTAAAGGACGGATGATAATT+TGG - chr1.3:58026433-58026452 MS.gene44068:intron 35.0%
TGAAAACAAATGCAAGGTCA+AGG - chr1.3:58026474-58026493 MS.gene44068:intron 35.0%
TGCATGCAAAAACCAGATAA+CGG + chr1.3:58026922-58026941 None:intergenic 35.0%
TGTTGAGCATTTGGATTATG+TGG - chr1.3:58025939-58025958 MS.gene44068:intron 35.0%
! ATCGAGGATATTTTCTCCTA+CGG + chr1.3:58026126-58026145 None:intergenic 35.0%
! CAATCTCAGAGTGTTTATTC+AGG + chr1.3:58025496-58025515 None:intergenic 35.0%
! CTTCATAGTTTGTGAACAGA+TGG + chr1.3:58026361-58026380 None:intergenic 35.0%
! GAAATGAGAGAGGTTGATTT+GGG + chr1.3:58025063-58025082 None:intergenic 35.0%
! GTTGTATTGTAATGGGAACA+CGG - chr1.3:58025843-58025862 MS.gene44068:intron 35.0%
! TAAACAAGGCAACACGTTTT+CGG - chr1.3:58026787-58026806 MS.gene44068:CDS 35.0%
! TGACCAAGTCTGATTTGAAA+CGG - chr1.3:58025331-58025350 MS.gene44068:intron 35.0%
!! GCAGTTGTGATCATTTTTAG+GGG - chr1.3:58026886-58026905 MS.gene44068:CDS 35.0%
AGTGAGTATGTGGTAAAGGA+CGG - chr1.3:58026421-58026440 MS.gene44068:intron 40.0%
AGTTGAGGTTGTGCTTTATC+AGG - chr1.3:58026568-58026587 MS.gene44068:intron 40.0%
ATCCAAATCCACTAGCCATT+AGG + chr1.3:58026180-58026199 None:intergenic 40.0%
ATGCAGTGAGTATGTGGTAA+AGG - chr1.3:58026417-58026436 MS.gene44068:intron 40.0%
CAACCGTTTCAAATCAGACT+TGG + chr1.3:58025337-58025356 None:intergenic 40.0%
CAATTCTCTCTTGTAGTTGG+AGG - chr1.3:58027104-58027123 MS.gene44068:CDS 40.0%
GAGCATTTGGATTATGTGGT+TGG - chr1.3:58025943-58025962 MS.gene44068:intron 40.0%
GATACCAACGCTCCAAAATT+TGG + chr1.3:58026010-58026029 None:intergenic 40.0%
GGACAATTCTCTCTTGTAGT+TGG - chr1.3:58027101-58027120 MS.gene44068:CDS 40.0%
GTTGAGGTTGTGCTTTATCA+GGG - chr1.3:58026569-58026588 MS.gene44068:intron 40.0%
TAAGGTTGTGCATGAAGGAA+GGG + chr1.3:58025145-58025164 None:intergenic 40.0%
TCTTGTAGTTGGAGGTTTGT+TGG - chr1.3:58027112-58027131 MS.gene44068:CDS 40.0%
TTACTCACTCATGCTCAACA+GGG - chr1.3:58025162-58025181 MS.gene44068:CDS 40.0%
TTGAGGTTGTGCTTTATCAG+GGG - chr1.3:58026570-58026589 MS.gene44068:intron 40.0%
! AAGGATTTACAAGACTGGCT+TGG - chr1.3:58026287-58026306 MS.gene44068:intron 40.0%
! GAAGATTTGAGGTTGAGAAG+TGG + chr1.3:58025027-58025046 None:intergenic 40.0%
! GAATCGTTTTGTCAGCATGT+GGG - chr1.3:58026503-58026522 MS.gene44068:intron 40.0%
! GGAAATGAGAGAGGTTGATT+TGG + chr1.3:58025064-58025083 None:intergenic 40.0%
! TGAATCGTTTTGTCAGCATG+TGG - chr1.3:58026502-58026521 MS.gene44068:intron 40.0%
! TGAGTTTGCGGACCAAATTT+TGG - chr1.3:58025995-58026014 MS.gene44068:intron 40.0%
! TTCAGCCCAGTTTTACTTAG+TGG - chr1.3:58025219-58025238 MS.gene44068:CDS 40.0%
! TTGGATTATGTGGTTGGTTG+TGG - chr1.3:58025949-58025968 MS.gene44068:intron 40.0%
!! ACTTCGGTGTTAGTTAGTTC+TGG - chr1.3:58025697-58025716 MS.gene44068:intron 40.0%
!! CTGGTTTTTGCATGCAGTAT+TGG - chr1.3:58026926-58026945 MS.gene44068:CDS 40.0%
!! TTCCTAATGGCTAGTGGATT+TGG - chr1.3:58026175-58026194 MS.gene44068:CDS 40.0%
!! TTTGCATGCAGTATTGGCTA+CGG - chr1.3:58026932-58026951 MS.gene44068:CDS 40.0%
!!! AGCTGATGCTTTTGAGTTTG+CGG - chr1.3:58025983-58026002 MS.gene44068:intron 40.0%
AAAGAAGGAGCAGTTACTGG+TGG - chr1.3:58026083-58026102 MS.gene44068:CDS 45.0%
ACCTTCCAGGTACTTCCTAA+TGG - chr1.3:58026162-58026181 MS.gene44068:CDS 45.0%
ACGTTTGTCGTCTTTAACCG+AGG + chr1.3:58027039-58027058 None:intergenic 45.0%
AGAAGTGGAGAGAGAGAGTT+GGG + chr1.3:58025012-58025031 None:intergenic 45.0%
AGAGAGAGTTGGGAGGAAAA+AGG + chr1.3:58025002-58025021 None:intergenic 45.0%
AGGTACTTCCTAATGGCTAG+TGG - chr1.3:58026169-58026188 MS.gene44068:CDS 45.0%
CAGAAAGAAGGAGCAGTTAC+TGG - chr1.3:58026080-58026099 MS.gene44068:CDS 45.0%
CATGTCATGCAGTGAGTATG+TGG - chr1.3:58026411-58026430 MS.gene44068:intron 45.0%
CGTTTGTCGTCTTTAACCGA+GGG + chr1.3:58027038-58027057 None:intergenic 45.0%
CTGTTGAGCATGAGTGAGTA+AGG + chr1.3:58025163-58025182 None:intergenic 45.0%
CTTACTCACTCATGCTCAAC+AGG - chr1.3:58025161-58025180 MS.gene44068:CDS 45.0%
GAAGAGACAGAAGAAAAGCG+TGG - chr1.3:58027080-58027099 MS.gene44068:CDS 45.0%
GAGAGAGTTGGGAGGAAAAA+GGG + chr1.3:58025001-58025020 None:intergenic 45.0%
GCAACATCAGAAGACAGACA+TGG + chr1.3:58026854-58026873 None:intergenic 45.0%
GCTTATGCGCCTACATACTT+CGG - chr1.3:58026762-58026781 MS.gene44068:CDS 45.0%
GGAGAGAAGGTGAAGATTTG+AGG + chr1.3:58025038-58025057 None:intergenic 45.0%
GGCACAATACTACCGTTATC+TGG - chr1.3:58026907-58026926 MS.gene44068:CDS 45.0%
GGGTTTGTAGGAAATGAGAG+AGG + chr1.3:58025073-58025092 None:intergenic 45.0%
GTAAGGTTGTGCATGAAGGA+AGG + chr1.3:58025146-58025165 None:intergenic 45.0%
GTGAGTAAGGTTGTGCATGA+AGG + chr1.3:58025150-58025169 None:intergenic 45.0%
GTGGAAGAAGGCAACGATAA+GGG - chr1.3:58026035-58026054 MS.gene44068:CDS 45.0%
TAATGAGCGTCCACCTAAAG+AGG - chr1.3:58025272-58025291 MS.gene44068:intron 45.0%
TCAACCACATAGGCCTCTTT+AGG + chr1.3:58025288-58025307 None:intergenic 45.0%
TCCAGCATCATCAACCACAT+AGG + chr1.3:58025298-58025317 None:intergenic 45.0%
TGGTATCCTTCTGTGGAAGA+AGG - chr1.3:58026023-58026042 MS.gene44068:CDS 45.0%
TGTGGAAGAAGGCAACGATA+AGG - chr1.3:58026034-58026053 MS.gene44068:CDS 45.0%
TGTGGGTGAATCACTTGTCA+CGG - chr1.3:58026520-58026539 MS.gene44068:intron 45.0%
! ACCTGGAAGGTTCTCTGAAA+CGG + chr1.3:58026153-58026172 None:intergenic 45.0%
!! ACTAGCCATTAGGAAGTACC+TGG + chr1.3:58026170-58026189 None:intergenic 45.0%
!! AGGGGCACTTTCAACATGAA+AGG - chr1.3:58026588-58026607 MS.gene44068:intron 45.0%
!! GTTGATTTGGGTTGGAGAGA+AGG + chr1.3:58025051-58025070 None:intergenic 45.0%
!! TGAGAGAGGTTGATTTGGGT+TGG + chr1.3:58025059-58025078 None:intergenic 45.0%
!!! TATTTTTATTATGTTATTAT+TGG + chr1.3:58025749-58025768 None:intergenic 5.0%
ACCACATAGGCCTCTTTAGG+TGG + chr1.3:58025285-58025304 None:intergenic 50.0%
ACCGTTTCAGAGAACCTTCC+AGG - chr1.3:58026149-58026168 MS.gene44068:CDS 50.0%
AGGATCTGGAAGTCCGTCAA+TGG + chr1.3:58027009-58027028 None:intergenic 50.0%
CCTACGGCTTGAACAAAAGC+AGG + chr1.3:58026110-58026129 None:intergenic 50.0%
GAGAAGTGGAGAGAGAGAGT+TGG + chr1.3:58025013-58025032 None:intergenic 50.0%
GGCAGCAACTAGTCGTTTAG+CGG + chr1.3:58026988-58027007 None:intergenic 50.0%
GTTGTGCATGAAGGAAGGGT+TGG + chr1.3:58025141-58025160 None:intergenic 50.0%
TCCACCTAAAGAGGCCTATG+TGG - chr1.3:58025281-58025300 MS.gene44068:intron 50.0%
TCGTTGCCTTCTTCCACAGA+AGG + chr1.3:58026032-58026051 None:intergenic 50.0%
! CGACTAGTTGCTGCCATTGA+CGG - chr1.3:58026993-58027012 MS.gene44068:CDS 50.0%
!! ATTGACGGACTTCCAGATCC+TGG - chr1.3:58027008-58027027 MS.gene44068:CDS 50.0%
!! CCTGCTTTTGTTCAAGCCGT+AGG - chr1.3:58026107-58026126 MS.gene44068:CDS 50.0%
!! GCCATTAGGAAGTACCTGGA+AGG + chr1.3:58026166-58026185 None:intergenic 50.0%
!! GCCTATGTGGTTGATGATGC+TGG - chr1.3:58025294-58025313 MS.gene44068:intron 50.0%
!! TCTCTGAAACGGTAGCATCG+AGG + chr1.3:58026142-58026161 None:intergenic 50.0%
!!! CGGACCAAATTTTGGAGCGT+TGG - chr1.3:58026003-58026022 MS.gene44068:intron 50.0%
AGTGGAGAGAGAGAGTTGGG+AGG + chr1.3:58025009-58025028 None:intergenic 55.0%
GTGCATGAAGGAAGGGTTGG+TGG + chr1.3:58025138-58025157 None:intergenic 55.0%
TCTTTAACCGAGGGACCACC+AGG + chr1.3:58027029-58027048 None:intergenic 55.0%
! AAGGCAACACGTTTTCGGCG+TGG - chr1.3:58026792-58026811 MS.gene44068:CDS 55.0%
!! AGGCAACGATAAGGGTGTCG+TGG - chr1.3:58026043-58026062 MS.gene44068:CDS 55.0%
!! GGAGCGTTGGTATCCTTCTG+TGG - chr1.3:58026016-58026035 MS.gene44068:CDS 55.0%
TCCAGATCCTGGTGGTCCCT+CGG - chr1.3:58027019-58027038 MS.gene44068:CDS 60.0%
! GACGGACTTCCAGATCCTGG+TGG - chr1.3:58027011-58027030 MS.gene44068:CDS 60.0%
ACCGAGGGACCACCAGGATC+TGG + chr1.3:58027023-58027042 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 58024991 58027190 58024991 ID=MS.gene44068
chr1.3 mRNA 58024991 58027190 58024991 ID=MS.gene44068.t1;Parent=MS.gene44068
chr1.3 exon 58026762 58027190 58026762 ID=MS.gene44068.t1.exon1;Parent=MS.gene44068.t1
chr1.3 CDS 58026762 58027190 58026762 ID=cds.MS.gene44068.t1;Parent=MS.gene44068.t1
chr1.3 exon 58026011 58026203 58026011 ID=MS.gene44068.t1.exon2;Parent=MS.gene44068.t1
chr1.3 CDS 58026011 58026203 58026011 ID=cds.MS.gene44068.t1;Parent=MS.gene44068.t1
chr1.3 exon 58024991 58025238 58024991 ID=MS.gene44068.t1.exon3;Parent=MS.gene44068.t1
chr1.3 CDS 58024991 58025238 58024991 ID=cds.MS.gene44068.t1;Parent=MS.gene44068.t1
Gene Sequence

>MS.gene44068

ATGACAACCCTTTTTCCTCCCAACTCTCTCTCTCCACTTCTCAACCTCAAATCTTCACCTTCTCTCCAACCCAAATCAACCTCTCTCATTTCCTACAAACCCATTGTTTCTAATCTCAAAAAGACTCAAACTTTATCTCTAAAACCACCAACCCTTCCTTCATGCACAACCTTACTCACTCATGCTCAACAGGGTCTTGCAGCTTTAGCTATCACTTTAGCATTGAATTTCAGCCCAGTTTTACTTAGTGGTAATGCATTGGCTTCTGAATTTGATGTGATTAATGAGCGTCCACCTAAAGAGGCCTATGTGGTTGATGATGCTGGAGTTCTTAGTAGAGTGACCAAGTCTGATTTGAAACGGTTGTTATCTGATTTGGAATCAAGGAAGAATTTTCATATCAATTTTATCACACTTAGAAAACTCACGGTTAGTTACGTAATCATTTATCTCATTGAATTAAATTAGGGTCGATTTGAATTAACTTATTTGAGTTTATCTACCTGAATAAACACTCTGAGATTGTTTGGAAGAGCTTATGGAACAGTTAGTGACATGTCCATTTGCAGTTTTTAGCTTATTTGCAGTTAGTTAACTTTATTTTATCTTTTGTTATAGAAATAACTTATACATTAGCAATTGCACAGTTATACCAAAAGAACTTAATTAAGTTGTTTATCAAAACATAGCCTAAATAAAGGAGAAAACTTCGGTGTTAGTTAGTTCTGGAGTTGTATTGTTTATCATGCTGTTCACCAATAATAACATAATAAAAATATTAATCTTTTTTAGTTAAATGTCAAATTAAAATAGTTCTGTTAATGTTTACAATTAAATAGAAAATCCATATGTGTTGTATTGTAATGGGAACACGGTAAATACAGCACACAAGTAACTGCACTTAAGTTTTGTTCATCAATACACTGATTAAATGGTGCAAAACAAAAATGTTGAGCATTTGGATTATGTGGTTGGTTGTGGTTGCAGAGTAAAGCTGATGCTTTTGAGTTTGCGGACCAAATTTTGGAGCGTTGGTATCCTTCTGTGGAAGAAGGCAACGATAAGGGTGTCGTGGTTCTTGTAACAAGTCAGAAAGAAGGAGCAGTTACTGGTGGACCTGCTTTTGTTCAAGCCGTAGGAGAAAATATCCTCGATGCTACCGTTTCAGAGAACCTTCCAGGTACTTCCTAATGGCTAGTGGATTTGGATTCTTGATTGTTTTTCTGTAGCATATTGGGGAGAGAATTACACTATTTGTATCACATAAGAACTTAATTATCATGATTATGTCTTTCAAAGGATTTACAAGACTGGCTTGGAGTTTTTTTTCTTCACTAACAAATATGGCAAGTGTTGGTTGCTGATTGCCATCTGTTCACAAACTATGAAGGAATCATCATTGATATGAATATGGTCATAACATGTCATGCAGTGAGTATGTGGTAAAGGACGGATGATAATTTGGATTCAAATATTTTTTTCTTGAAAACAAATGCAAGGTCAAGGACATGTGAATCGTTTTGTCAGCATGTGGGTGAATCACTTGTCACGGCACTTTTTTTTTTTTTTTGGTTAAGAGTTGAGGTTGTGCTTTATCAGGGGCACTTTCAACATGAAAGGTTGCAGACACTACAACGCTCAATGATCACAACGACCATAAAGATAATGATGGATATGTTTTCACTTTTAGAAATCAATACATGATAATCCCGTTTTAGCTATCACAAAAAACATGTTTCCTATCCATTTAATTTCAGTGTATGTTGTCAAGGCTTATGCGCCTACATACTTCGGTTTAAACAAGGCAACACGTTTTCGGCGTGGTGATAAACTAGAGTACAAGTTTGTATTATTTATAAACCATGTCTGTCTTCTGATGTTGCATTCATTAAGTAGCAGTTGTGATCATTTTTAGGGGCACAATACTACCGTTATCTGGTTTTTGCATGCAGTATTGGCTACGGATGAGAAGTACAATGAAGCAATTTATAGCACCGCTAAACGACTAGTTGCTGCCATTGACGGACTTCCAGATCCTGGTGGTCCCTCGGTTAAAGACGACAAACGTGAGTCTAACTTCAAAACAAAGGAAGAGACAGAAGAAAAGCGTGGACAATTCTCTCTTGTAGTTGGAGGTTTGTTGGTTATTGCATTTGTTGTTCCTATGGTACAATACTATGCTTATGTAGCCAAAAAATAA
Protein sequence

>MS.gene44068.t1

MTTLFPPNSLSPLLNLKSSPSLQPKSTSLISYKPIVSNLKKTQTLSLKPPTLPSCTTLLTHAQQGLAALAITLALNFSPVLLSGNALASEFDVINERPPKEAYVVDDAGVLSRVTKSDLKRLLSDLESRKNFHINFITLRKLTSKADAFEFADQILERWYPSVEEGNDKGVVVLVTSQKEGAVTGGPAFVQAVGENILDATVSENLPVLATDEKYNEAIYSTAKRLVAAIDGLPDPGGPSVKDDKRESNFKTKEETEEKRGQFSLVVGGLLVIAFVVPMVQYYAYVAKK