AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0224690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0224690 MtrunA17_Chr2g0330781 98.235 170 3 0 1 170 1 170 1.11e-119 342
Msa0224690 MtrunA17_Chr8g0341001 75.882 170 41 0 1 170 2 171 6.69e-90 266
Msa0224690 MtrunA17_Chr2g0320671 57.764 161 68 0 10 170 9 169 4.77e-59 187
Msa0224690 MtrunA17_Chr7g0229591 52.830 159 75 0 11 169 10 168 2.42e-51 163
Msa0224690 MtrunA17_Chr4g0027521 54.930 142 64 0 28 169 24 165 1.17e-47 158
Msa0224690 MtrunA17_Chr1g0156931 47.239 163 84 1 9 169 17 179 1.01e-45 153
Msa0224690 MtrunA17_Chr3g0120861 51.408 142 69 0 29 170 36 177 7.22e-45 151
Msa0224690 MtrunA17_Chr5g0438621 49.296 142 72 0 29 170 28 169 2.38e-43 147
Msa0224690 MtrunA17_Chr5g0438631 43.919 148 83 0 23 170 20 167 5.14e-41 140
Msa0224690 MtrunA17_Chr3g0105401 42.941 170 93 1 1 170 2 167 1.60e-40 139
Msa0224690 MtrunA17_Chr5g0438641 47.183 142 75 0 29 170 30 171 2.20e-40 139
Msa0224690 MtrunA17_Chr4g0046151 44.286 140 78 0 31 170 36 175 2.89e-40 139
Msa0224690 MtrunA17_Chr5g0432101 46.429 140 75 0 31 170 33 172 7.13e-40 138
Msa0224690 MtrunA17_Chr2g0283191 41.317 167 95 2 7 170 14 180 1.00e-39 137
Msa0224690 MtrunA17_Chr8g0371481 45.390 141 77 0 30 170 40 180 1.64e-39 137
Msa0224690 MtrunA17_Chr8g0371471 44.681 141 78 0 30 170 40 180 2.34e-38 134
Msa0224690 MtrunA17_Chr3g0142941 45.139 144 79 0 26 169 42 185 5.56e-38 133
Msa0224690 MtrunA17_Chr8g0371491 44.138 145 81 0 25 169 30 174 1.31e-37 132
Msa0224690 MtrunA17_Chr6g0469511 54.237 118 54 0 26 143 25 142 1.57e-37 125
Msa0224690 MtrunA17_Chr5g0405821 41.916 167 92 3 9 170 18 184 3.33e-37 131
Msa0224690 MtrunA17_Chr1g0156901 41.566 166 97 0 5 170 24 189 1.07e-36 130
Msa0224690 MtrunA17_Chr5g0405831 40.606 165 94 2 9 169 18 182 3.90e-36 128
Msa0224690 MtrunA17_Chr1g0157001 42.647 136 77 1 36 170 41 176 3.38e-34 123
Msa0224690 MtrunA17_Chr1g0156911 41.844 141 82 0 30 170 36 176 7.53e-34 122
Msa0224690 MtrunA17_Chr1g0156891 44.056 143 79 1 29 170 41 183 1.15e-33 122
Msa0224690 MtrunA17_Chr4g0027561 57.576 99 42 0 26 124 25 123 1.20e-33 116
Msa0224690 MtrunA17_Chr1g0156921 37.654 162 101 0 9 170 20 181 6.32e-33 120
Msa0224690 MtrunA17_Chr3g0142921 42.254 142 81 1 30 170 35 176 1.92e-31 115
Msa0224690 MtrunA17_Chr6g0457921 41.975 162 90 4 10 168 7 167 2.41e-31 115
Msa0224690 MtrunA17_Chr7g0274991 40.244 164 91 3 11 168 8 170 7.33e-31 114
Msa0224690 MtrunA17_Chr7g0275021 43.243 148 80 3 23 168 26 171 1.01e-30 114
Msa0224690 MtrunA17_Chr7g0275011 43.165 139 77 2 31 168 34 171 1.77e-30 113
Msa0224690 MtrunA17_Chr1g0182811 42.446 139 79 1 31 168 33 171 2.30e-30 113
Msa0224690 MtrunA17_Chr5g0437421 40.141 142 85 0 29 170 30 171 8.01e-30 111
Msa0224690 MtrunA17_Chr1g0189891 45.000 140 74 3 31 168 29 167 1.91e-29 110
Msa0224690 MtrunA17_Chr1g0189931 41.935 155 85 3 15 168 19 169 2.29e-29 110
Msa0224690 MtrunA17_Chr1g0189901 43.165 139 77 2 31 168 31 168 4.29e-29 109
Msa0224690 MtrunA17_Chr7g0275001 39.645 169 94 3 1 168 1 162 4.30e-29 109
Msa0224690 MtrunA17_Chr4g0008851 38.690 168 98 4 2 168 6 169 1.95e-28 108
Msa0224690 MtrunA17_Chr3g0099161 42.262 168 79 8 5 162 67 226 4.67e-27 105
Msa0224690 MtrunA17_Chr2g0285061 41.176 170 90 6 3 170 9 170 1.48e-26 103
Msa0224690 MtrunA17_Chr1g0189921 48.148 108 46 2 31 129 31 137 1.09e-25 96.3
Msa0224690 MtrunA17_Chr3g0099151 38.636 176 94 7 5 170 62 233 1.97e-25 100
Msa0224690 MtrunA17_Chr8g0374101 40.000 140 80 3 35 170 41 180 4.37e-25 99.4
Msa0224690 MtrunA17_Chr2g0294301 39.161 143 83 3 29 170 33 172 4.43e-25 99.0
Msa0224690 MtrunA17_Chr3g0086641 42.105 152 78 6 10 156 15 161 6.22e-25 99.0
Msa0224690 MtrunA17_Chr7g0215961 36.420 162 87 4 9 168 10 157 7.27e-25 98.6
Msa0224690 MtrunA17_Chr5g0444401 41.333 150 75 6 10 151 11 155 9.20e-25 98.2
Msa0224690 MtrunA17_Chr8g0380041 39.024 164 94 4 9 170 9 168 1.57e-24 97.4
Msa0224690 MtrunA17_Chr2g0284921 38.776 147 85 3 27 170 30 174 5.58e-24 96.3
Msa0224690 MtrunA17_Chr4g0068441 36.095 169 87 6 9 170 18 172 8.01e-24 95.9
Msa0224690 MtrunA17_Chr3g0099231 38.068 176 96 8 3 169 2 173 8.80e-24 95.9
Msa0224690 MtrunA17_Chr8g0380031 38.462 143 84 3 29 170 82 221 2.47e-22 92.0
Msa0224690 MtrunA17_Chr3g0077571 37.654 162 93 5 10 170 12 166 3.28e-22 91.3
Msa0224690 MtrunA17_Chr2g0284941 38.889 144 83 4 29 170 31 171 4.83e-22 90.9
Msa0224690 MtrunA17_Chr2g0284951 38.889 144 83 4 29 170 31 171 1.11e-21 90.1
Msa0224690 MtrunA17_Chr5g0396511 34.559 136 87 2 34 168 49 183 1.91e-21 89.4
Msa0224690 MtrunA17_Chr2g0284991 38.889 144 83 4 29 170 31 171 5.73e-21 88.2
Msa0224690 MtrunA17_Chr3g0131321 38.519 135 79 3 31 162 34 167 1.09e-20 87.4
Msa0224690 MtrunA17_Chr8g0370981 34.177 158 90 3 23 170 36 189 1.51e-20 87.0
Msa0224690 MtrunA17_Chr4g0017431 34.266 143 91 3 29 170 29 169 4.84e-20 85.5
Msa0224690 MtrunA17_Chr3g0077581 47.368 95 47 2 29 123 34 125 6.43e-20 85.1
Msa0224690 MtrunA17_Chr4g0018561 52.174 69 33 0 12 80 11 79 7.10e-20 79.7
Msa0224690 MtrunA17_Chr5g0420811 38.667 150 86 5 22 168 24 170 5.62e-19 82.4
Msa0224690 MtrunA17_Chr2g0301641 38.732 142 80 4 31 170 43 179 9.15e-19 82.0
Msa0224690 MtrunA17_Chr2g0321991 48.889 90 44 2 29 117 31 119 8.15e-18 75.1
Msa0224690 MtrunA17_Chr2g0285001 33.929 168 101 6 10 170 8 172 8.23e-18 79.3
Msa0224690 MtrunA17_Chr2g0294311 32.895 152 88 4 30 170 31 179 6.38e-17 76.6
Msa0224690 MtrunA17_Chr8g0349341 35.593 177 90 9 7 168 4 171 8.09e-17 76.3
Msa0224690 MtrunA17_Chr3g0085881 35.417 144 87 5 29 170 31 170 8.27e-17 76.3
Msa0224690 MtrunA17_Chr6g0455661 43.590 117 60 4 1 117 3 113 9.12e-17 76.6
Msa0224690 MtrunA17_Chr5g0434621 35.507 138 86 3 35 170 29 165 1.17e-16 75.9
Msa0224690 MtrunA17_Chr3g0085231 35.754 179 95 9 3 170 1 170 1.38e-16 75.9
Msa0224690 MtrunA17_Chr8g0349301 32.044 181 94 8 7 170 4 172 1.54e-16 75.5
Msa0224690 MtrunA17_Chr8g0370971 38.053 113 66 1 9 117 16 128 3.40e-16 72.0
Msa0224690 MtrunA17_Chr8g0349321 31.977 172 95 8 9 166 6 169 5.55e-15 71.2
Msa0224690 MtrunA17_Chr8g0344931 33.099 142 88 4 31 170 37 173 9.46e-15 70.5
Msa0224690 MtrunA17_Chr8g0349331 33.125 160 93 6 9 162 13 164 1.35e-14 70.1
Msa0224690 MtrunA17_Chr4g0060811 33.813 139 88 4 35 170 31 168 2.48e-14 69.3
Msa0224690 MtrunA17_Chr8g0370951 33.533 167 102 6 12 170 11 176 3.75e-14 68.9
Msa0224690 MtrunA17_Chr8g0344941 33.099 142 88 5 31 170 41 177 1.51e-13 67.4
Msa0224690 MtrunA17_Chr2g0301581 33.333 174 94 9 3 165 7 169 6.86e-13 65.5
Msa0224690 MtrunA17_Chr2g0301541 33.333 174 94 9 3 165 7 169 7.00e-13 65.5
Msa0224690 MtrunA17_Chr6g0463261 36.667 90 57 0 81 170 1 90 1.13e-12 61.6
Msa0224690 MtrunA17_Chr1g0160011 36.000 125 65 6 30 148 39 154 8.53e-12 62.4
Msa0224690 MtrunA17_Chr7g0254461 37.500 120 63 5 30 144 42 154 1.50e-11 61.6
Msa0224690 MtrunA17_Chr2g0301591 33.333 153 88 7 3 147 7 153 2.69e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0224690 AT3G16370.1 79.720 143 29 0 28 170 27 169 2.46e-78 237
Msa0224690 AT5G03810.1 56.725 171 71 2 1 170 1 169 1.41e-58 186
Msa0224690 AT5G03820.1 58.385 161 65 1 10 170 14 172 1.82e-58 186
Msa0224690 AT5G03820.2 57.310 171 70 2 1 170 32 200 7.56e-58 185
Msa0224690 AT5G22810.1 57.485 167 66 2 9 170 10 176 5.36e-57 182
Msa0224690 AT3G53100.1 52.448 143 68 0 28 170 26 168 7.81e-51 166
Msa0224690 AT2G04570.1 50.704 142 70 0 29 170 26 167 2.83e-46 154
Msa0224690 AT2G42990.1 47.887 142 74 0 29 170 26 167 2.52e-43 147
Msa0224690 AT1G20120.1 49.306 144 73 0 26 169 73 216 3.67e-42 145
Msa0224690 AT5G42170.1 46.897 145 77 0 26 170 45 189 1.24e-41 143
Msa0224690 AT5G45960.1 45.070 142 78 0 29 170 45 186 4.94e-39 136
Msa0224690 AT1G75890.2 43.195 169 89 2 9 170 19 187 2.71e-38 134
Msa0224690 AT1G75890.3 43.195 169 89 2 9 170 48 216 4.64e-38 134
Msa0224690 AT5G63170.1 45.455 143 77 1 29 170 26 168 7.05e-38 132
Msa0224690 AT4G26790.1 44.681 141 78 0 30 170 28 168 1.82e-37 132
Msa0224690 AT4G26790.2 44.681 141 78 0 30 170 28 168 1.82e-37 132
Msa0224690 AT2G24560.2 43.919 148 82 1 22 168 25 172 2.66e-37 130
Msa0224690 AT5G45950.1 44.366 142 78 1 29 170 38 178 5.64e-37 130
Msa0224690 AT2G24560.1 43.919 148 82 1 22 168 25 172 6.11e-37 130
Msa0224690 AT1G58430.1 40.361 166 94 2 8 168 8 173 6.49e-37 130
Msa0224690 AT1G75880.2 40.828 169 93 1 9 170 23 191 1.23e-36 130
Msa0224690 AT2G30310.1 41.892 148 85 1 22 168 25 172 2.71e-36 129
Msa0224690 AT3G14820.1 43.056 144 82 0 27 170 28 171 3.68e-36 128
Msa0224690 AT1G75900.1 43.662 142 80 0 28 169 40 181 5.78e-36 128
Msa0224690 AT1G06990.1 40.972 144 85 0 27 170 33 176 5.97e-36 128
Msa0224690 AT2G30220.1 39.521 167 97 2 6 168 5 171 7.98e-36 127
Msa0224690 AT1G75880.1 41.176 170 92 2 9 170 23 192 1.85e-35 127
Msa0224690 AT2G40250.1 38.857 175 102 1 1 170 1 175 2.53e-35 126
Msa0224690 AT1G75890.1 40.110 182 89 3 9 170 19 200 4.83e-35 126
Msa0224690 AT2G31540.1 42.667 150 85 1 22 170 26 175 8.16e-35 125
Msa0224690 AT1G59406.1 38.824 170 103 1 1 170 1 169 1.39e-34 124
Msa0224690 AT1G59030.1 38.824 170 103 1 1 170 1 169 1.39e-34 124
Msa0224690 AT1G58725.1 38.824 170 103 1 1 170 1 169 1.39e-34 124
Msa0224690 AT3G43550.1 40.690 145 86 0 26 170 25 169 2.46e-34 122
Msa0224690 AT3G43570.1 40.000 145 87 0 26 170 25 169 6.71e-34 122
Msa0224690 AT1G73610.1 39.429 175 99 3 3 170 1 175 1.21e-33 122
Msa0224690 AT1G58520.3 40.000 145 87 0 26 170 25 169 5.92e-33 120
Msa0224690 AT1G58525.1 40.000 145 87 0 26 170 25 169 5.92e-33 120
Msa0224690 AT4G28780.1 43.537 147 79 3 24 168 24 168 2.96e-32 118
Msa0224690 AT1G75920.2 40.252 159 94 1 12 170 11 168 4.36e-32 117
Msa0224690 AT3G04290.1 44.604 139 75 2 31 168 29 166 2.46e-31 116
Msa0224690 AT5G18430.1 41.007 139 81 1 31 168 28 166 5.14e-30 112
Msa0224690 AT5G33370.2 44.286 140 74 3 31 168 30 167 3.36e-29 109
Msa0224690 AT1G20130.2 38.462 156 86 1 25 170 178 333 3.55e-29 112
Msa0224690 AT1G20130.1 38.462 156 86 1 25 170 198 353 3.88e-29 112
Msa0224690 AT5G33370.1 44.286 140 74 3 31 168 30 167 8.32e-29 109
Msa0224690 AT1G23500.1 42.945 163 86 2 9 170 19 175 1.47e-28 108
Msa0224690 AT1G75920.1 37.356 174 93 2 12 170 6 178 1.54e-28 108
Msa0224690 AT1G20135.1 37.654 162 99 2 10 170 9 169 1.91e-28 107
Msa0224690 AT1G75910.1 40.426 141 84 0 30 170 27 167 2.07e-28 108
Msa0224690 AT1G20132.1 35.862 145 93 0 26 170 56 200 9.42e-28 107
Msa0224690 AT5G41890.1 39.568 139 81 2 35 170 35 173 1.95e-27 105
Msa0224690 AT1G29670.1 39.157 166 93 5 9 170 8 169 3.81e-27 105
Msa0224690 AT1G29670.2 39.157 166 93 5 9 170 76 237 9.80e-27 104
Msa0224690 AT1G29660.1 37.572 173 101 5 1 170 1 169 2.50e-26 102
Msa0224690 AT3G50400.1 38.650 163 91 4 6 159 3 165 2.74e-26 102
Msa0224690 AT4G16230.1 39.161 143 84 2 29 170 28 168 9.82e-26 99.0
Msa0224690 AT4G10950.2 35.294 153 91 3 23 168 57 208 3.83e-25 100
Msa0224690 AT5G15720.2 52.427 103 45 3 16 117 14 113 6.07e-24 95.5
Msa0224690 AT5G15720.1 52.427 103 45 3 16 117 14 113 1.58e-23 95.1
Msa0224690 AT1G75930.1 38.667 150 90 2 23 170 21 170 3.93e-23 94.0
Msa0224690 AT5G08460.1 34.756 164 102 4 9 170 28 188 5.09e-23 94.0
Msa0224690 AT5G45670.1 38.562 153 89 4 6 156 3 152 6.52e-23 93.6
Msa0224690 AT4G18970.1 38.776 147 85 4 26 170 23 166 8.50e-23 93.2
Msa0224690 AT4G18970.2 38.776 147 85 4 26 170 23 166 1.31e-22 93.2
Msa0224690 AT4G10950.1 34.641 153 88 4 23 168 57 204 1.77e-22 92.8
Msa0224690 AT1G33811.1 38.849 139 77 4 5 138 4 139 9.59e-22 90.5
Msa0224690 AT2G23540.1 40.157 127 73 2 35 158 52 178 1.69e-21 90.1
Msa0224690 AT1G71691.3 38.776 147 84 5 26 170 50 192 7.03e-21 87.4
Msa0224690 AT1G71691.2 38.776 147 84 5 26 170 50 192 1.64e-20 87.4
Msa0224690 AT4G16220.1 45.536 112 57 2 6 117 7 114 8.96e-20 83.2
Msa0224690 AT5G55050.1 40.000 130 71 3 29 151 37 166 2.19e-19 84.0
Msa0224690 AT1G53920.1 38.776 147 84 5 26 170 43 185 6.03e-19 82.8
Msa0224690 AT1G75920.3 32.075 159 76 2 12 170 11 137 6.04e-19 82.0
Msa0224690 AT1G75920.4 38.462 117 72 0 54 170 24 140 6.83e-19 82.0
Msa0224690 AT2G19050.2 35.915 142 83 4 29 170 29 162 1.05e-17 78.6
Msa0224690 AT2G19010.1 34.043 141 85 4 30 170 29 161 1.51e-17 78.6
Msa0224690 AT2G19050.1 35.915 142 83 4 29 170 29 162 1.74e-17 78.6
Msa0224690 AT2G19010.2 34.043 141 85 4 30 170 59 191 1.80e-17 78.6
Msa0224690 AT1G71250.1 34.965 143 90 3 29 170 39 179 2.40e-17 78.2
Msa0224690 AT2G03980.2 41.593 113 63 3 30 140 42 153 2.04e-16 75.9
Msa0224690 AT2G03980.3 41.593 113 63 3 30 140 42 153 2.04e-16 75.9
Msa0224690 AT2G03980.1 41.593 113 63 3 30 140 42 153 2.04e-16 75.9
Msa0224690 AT2G03980.4 41.593 113 63 3 30 140 42 153 2.04e-16 75.9
Msa0224690 AT2G04020.1 41.525 118 64 4 30 144 42 157 2.44e-16 74.3
Msa0224690 AT2G04020.2 41.525 118 64 4 30 144 42 157 4.91e-16 74.3
Msa0224690 AT5G37690.1 34.058 138 88 3 35 170 31 167 7.87e-16 73.9
Msa0224690 AT1G53940.1 32.386 176 105 6 4 168 3 175 8.75e-16 73.6
Msa0224690 AT1G53940.2 33.898 177 101 8 4 168 3 175 1.55e-15 73.2
Msa0224690 AT1G71120.1 35.948 153 85 5 23 170 23 167 7.87e-15 71.2
Msa0224690 AT4G30140.1 32.624 141 87 3 30 170 32 164 8.08e-15 71.2
Msa0224690 AT2G19060.1 33.566 143 87 4 28 170 27 161 9.14e-15 70.9
Msa0224690 AT1G74460.1 33.133 166 102 6 8 170 5 164 1.95e-13 67.4
Msa0224690 AT1G28600.2 33.140 172 90 9 11 167 8 169 3.79e-13 65.1
Msa0224690 AT3G14225.1 31.034 145 93 4 31 170 37 179 6.69e-13 65.9
Msa0224690 AT1G28600.1 32.948 173 91 9 10 167 7 169 1.65e-12 64.7
Msa0224690 AT1G28580.1 35.616 146 76 6 31 165 37 175 5.21e-12 63.2
Msa0224690 AT1G28610.1 31.792 173 94 7 8 167 9 170 9.33e-12 61.6
Msa0224690 AT1G28590.1 34.899 149 73 7 31 165 36 174 1.24e-11 62.4
Msa0224690 AT5G37690.2 32.609 138 82 4 35 170 31 159 1.32e-11 62.0
Msa0224690 AT1G28570.1 31.928 166 102 6 8 165 10 172 1.53e-11 62.0
Msa0224690 AT1G28610.2 31.792 173 94 7 8 167 9 170 1.73e-11 61.6
Msa0224690 AT5G14450.1 32.075 159 85 8 1 144 1 151 9.30e-11 59.7

Find 34 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACCTCCCAGTGGGTTTA+TGG 0.171244 2_1:+74590204 None:intergenic
AAAGCTAACTATCCTCCTTA+TGG 0.312317 2_1:-74590242 Msa0224690:CDS
CAAGGTAGCTGGAAGTAAAA+AGG 0.359520 2_1:-74589705 Msa0224690:CDS
AAGGCAGCATCAATTATTAA+AGG 0.375056 2_1:-74589686 Msa0224690:CDS
TCACCAAACGTCACGATTGC+CGG 0.392033 2_1:+74590311 None:intergenic
TGGTGACTCTGCTGTGGATG+TGG 0.414876 2_1:-74590294 Msa0224690:CDS
CAAAATTTGCTCCAATAAGA+AGG 0.415639 2_1:+74589904 None:intergenic
AAATTAGCTACTGATATTAC+TGG 0.418320 2_1:-74590173 Msa0224690:intron
GGTGACTCTGCTGTGGATGT+GGG 0.428006 2_1:-74590293 Msa0224690:CDS
GTTCCGGCAATCGTGACGTT+TGG 0.433443 2_1:-74590314 Msa0224690:CDS
TATCTTAGCCCTCAAGCAAC+AGG 0.436881 2_1:-74589933 Msa0224690:CDS
CTACCTTGGAAAGCTTACTT+TGG 0.474075 2_1:+74589720 None:intergenic
CTAACAAAGTCTCTTCCATA+AGG 0.478982 2_1:+74590227 None:intergenic
TCTTTATGTAGCTGAAACAC+TGG 0.483722 2_1:-74589979 Msa0224690:intron
TGCATCTGGTTATGATGAAA+AGG 0.486563 2_1:-74589874 Msa0224690:CDS
AGTAAGCTTTCCAAGGTAGC+TGG 0.499862 2_1:-74589716 Msa0224690:CDS
ACAAGATGATACCGTTGTTC+CGG 0.509338 2_1:-74590330 Msa0224690:CDS
TCTCTCTAGCTAACAACACT+AGG 0.536448 2_1:+74590424 None:intergenic
CTTTATGTAGCTGAAACACT+GGG 0.542754 2_1:-74589978 Msa0224690:intron
TTTGTTAGCCATAAACCCAC+TGG 0.558300 2_1:-74590212 Msa0224690:CDS
CCATTGCAAAACCTCCCAGT+GGG 0.560224 2_1:+74590197 None:intergenic
GCTTGAGGGCTAAGATATGC+AGG 0.564871 2_1:+74589939 None:intergenic
GTGACTCTGCTGTGGATGTG+GGG 0.573179 2_1:-74590292 Msa0224690:CDS
GACGTTTGGTGACTCTGCTG+TGG 0.577397 2_1:-74590300 Msa0224690:CDS
CGTCACGATTGCCGGAACAA+CGG 0.582020 2_1:+74590319 None:intergenic
GCATCAGTTAAAATATTACA+AGG 0.605021 2_1:-74589747 Msa0224690:CDS
ATACCAAAGTAAGCTTTCCA+AGG 0.615809 2_1:-74589723 Msa0224690:CDS
ACAAAGTCTCTTCCATAAGG+AGG 0.615968 2_1:+74590230 None:intergenic
TAACAACACTAGGTTTCTGA+AGG 0.619039 2_1:+74590434 None:intergenic
TTGTTAGCCATAAACCCACT+GGG 0.652946 2_1:-74590211 Msa0224690:CDS
GCCATTGCAAAACCTCCCAG+TGG 0.660851 2_1:+74590196 None:intergenic
GCATTGTACCTATTGAGTGG+TGG 0.691170 2_1:-74589662 Msa0224690:CDS
TTAGCCATAAACCCACTGGG+AGG 0.727905 2_1:-74590208 Msa0224690:CDS
GGTGCATTGTACCTATTGAG+TGG 0.748700 2_1:-74589665 Msa0224690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAGAAAAAATTAATATTG+CGG + chr2_1:74589661-74589680 None:intergenic 15.0%
!! TGTAGAAAAAATTAATATTG+CGG + chr2_1:74589661-74589680 None:intergenic 15.0%
! AAATTAGCTACTGATATTAC+TGG - chr2_1:74589974-74589993 Msa0224690:intron 25.0%
!! AAATGTTAGTATTTTCTCCA+TGG - chr2_1:74590063-74590082 Msa0224690:intron 25.0%
!! AATGTTAGTATTTTCTCCAT+GGG - chr2_1:74590064-74590083 Msa0224690:intron 25.0%
!!! ATTAGTATTAGAAGTGTTGA+AGG + chr2_1:74590113-74590132 None:intergenic 25.0%
! GCATCAGTTAAAATATTACA+AGG - chr2_1:74590400-74590419 Msa0224690:CDS 25.0%
! AAATTAGCTACTGATATTAC+TGG - chr2_1:74589974-74589993 Msa0224690:intron 25.0%
!! AAATGTTAGTATTTTCTCCA+TGG - chr2_1:74590063-74590082 Msa0224690:intron 25.0%
!! AATGTTAGTATTTTCTCCAT+GGG - chr2_1:74590064-74590083 Msa0224690:intron 25.0%
!!! ATTAGTATTAGAAGTGTTGA+AGG + chr2_1:74590113-74590132 None:intergenic 25.0%
! GCATCAGTTAAAATATTACA+AGG - chr2_1:74590400-74590419 Msa0224690:CDS 25.0%
! TGTGTTTGCATTTGTGTTTT+TGG - chr2_1:74589781-74589800 Msa0224690:intron 30.0%
! GTGTTTGCATTTGTGTTTTT+GGG - chr2_1:74589782-74589801 Msa0224690:intron 30.0%
!! AAGTGTTGAAGGTCTTAAAA+TGG + chr2_1:74590102-74590121 None:intergenic 30.0%
ACAGGAAAAAACCTTCTTAT+TGG - chr2_1:74590232-74590251 Msa0224690:CDS 30.0%
! CAAAATTTGCTCCAATAAGA+AGG + chr2_1:74590246-74590265 None:intergenic 30.0%
!!! TATTTTAACTGATGCGACAA+TGG + chr2_1:74590395-74590414 None:intergenic 30.0%
AAGGCAGCATCAATTATTAA+AGG - chr2_1:74590461-74590480 Msa0224690:five_prime_UTR 30.0%
! TGTGTTTGCATTTGTGTTTT+TGG - chr2_1:74589781-74589800 Msa0224690:intron 30.0%
! GTGTTTGCATTTGTGTTTTT+GGG - chr2_1:74589782-74589801 Msa0224690:intron 30.0%
!! AAGTGTTGAAGGTCTTAAAA+TGG + chr2_1:74590102-74590121 None:intergenic 30.0%
ACAGGAAAAAACCTTCTTAT+TGG - chr2_1:74590232-74590251 Msa0224690:CDS 30.0%
! CAAAATTTGCTCCAATAAGA+AGG + chr2_1:74590246-74590265 None:intergenic 30.0%
!!! TATTTTAACTGATGCGACAA+TGG + chr2_1:74590395-74590414 None:intergenic 30.0%
AAGGCAGCATCAATTATTAA+AGG - chr2_1:74590461-74590480 Msa0224690:five_prime_UTR 30.0%
TAACAACACTAGGTTTCTGA+AGG + chr2_1:74589716-74589735 None:intergenic 35.0%
!!! TTGCATTTGTGTTTTTGGGA+TGG - chr2_1:74589786-74589805 Msa0224690:intron 35.0%
!!! TGCATTTGTGTTTTTGGGAT+GGG - chr2_1:74589787-74589806 Msa0224690:intron 35.0%
AAAGCTAACTATCCTCCTTA+TGG - chr2_1:74589905-74589924 Msa0224690:CDS 35.0%
CTAACAAAGTCTCTTCCATA+AGG + chr2_1:74589923-74589942 None:intergenic 35.0%
TAAGAGAGTTGCATGTTTGA+TGG + chr2_1:74590011-74590030 None:intergenic 35.0%
TCTTTATGTAGCTGAAACAC+TGG - chr2_1:74590168-74590187 Msa0224690:intron 35.0%
CTTTATGTAGCTGAAACACT+GGG - chr2_1:74590169-74590188 Msa0224690:intron 35.0%
TGCATCTGGTTATGATGAAA+AGG - chr2_1:74590273-74590292 Msa0224690:CDS 35.0%
ATACCAAAGTAAGCTTTCCA+AGG - chr2_1:74590424-74590443 Msa0224690:five_prime_UTR 35.0%
TAACAACACTAGGTTTCTGA+AGG + chr2_1:74589716-74589735 None:intergenic 35.0%
!!! TTGCATTTGTGTTTTTGGGA+TGG - chr2_1:74589786-74589805 Msa0224690:intron 35.0%
!!! TGCATTTGTGTTTTTGGGAT+GGG - chr2_1:74589787-74589806 Msa0224690:intron 35.0%
AAAGCTAACTATCCTCCTTA+TGG - chr2_1:74589905-74589924 Msa0224690:CDS 35.0%
CTAACAAAGTCTCTTCCATA+AGG + chr2_1:74589923-74589942 None:intergenic 35.0%
TAAGAGAGTTGCATGTTTGA+TGG + chr2_1:74590011-74590030 None:intergenic 35.0%
TCTTTATGTAGCTGAAACAC+TGG - chr2_1:74590168-74590187 Msa0224690:intron 35.0%
CTTTATGTAGCTGAAACACT+GGG - chr2_1:74590169-74590188 Msa0224690:intron 35.0%
TGCATCTGGTTATGATGAAA+AGG - chr2_1:74590273-74590292 Msa0224690:CDS 35.0%
ATACCAAAGTAAGCTTTCCA+AGG - chr2_1:74590424-74590443 Msa0224690:five_prime_UTR 35.0%
TCTCTCTAGCTAACAACACT+AGG + chr2_1:74589726-74589745 None:intergenic 40.0%
!!! GCATTTGTGTTTTTGGGATG+GGG - chr2_1:74589788-74589807 Msa0224690:intron 40.0%
!!! CATTTGTGTTTTTGGGATGG+GGG - chr2_1:74589789-74589808 Msa0224690:intron 40.0%
ACAAGATGATACCGTTGTTC+CGG - chr2_1:74589817-74589836 Msa0224690:intron 40.0%
ACAAAGTCTCTTCCATAAGG+AGG + chr2_1:74589920-74589939 None:intergenic 40.0%
TTTGTTAGCCATAAACCCAC+TGG - chr2_1:74589935-74589954 Msa0224690:CDS 40.0%
TTGTTAGCCATAAACCCACT+GGG - chr2_1:74589936-74589955 Msa0224690:CDS 40.0%
TTCTCCATGGGTTTGAATCT+CGG - chr2_1:74590076-74590095 Msa0224690:intron 40.0%
!!! GGTTTTTTCCTGTTGCTTGA+GGG + chr2_1:74590225-74590244 None:intergenic 40.0%
!!! AGGTTTTTTCCTGTTGCTTG+AGG + chr2_1:74590226-74590245 None:intergenic 40.0%
! AATTTTGCTTCAGCTGCATC+TGG - chr2_1:74590259-74590278 Msa0224690:CDS 40.0%
CTACCTTGGAAAGCTTACTT+TGG + chr2_1:74590430-74590449 None:intergenic 40.0%
! CTTTTTACTTCCAGCTACCT+TGG + chr2_1:74590444-74590463 None:intergenic 40.0%
CAAGGTAGCTGGAAGTAAAA+AGG - chr2_1:74590442-74590461 Msa0224690:five_prime_UTR 40.0%
TCTCTCTAGCTAACAACACT+AGG + chr2_1:74589726-74589745 None:intergenic 40.0%
!!! GCATTTGTGTTTTTGGGATG+GGG - chr2_1:74589788-74589807 Msa0224690:intron 40.0%
!!! CATTTGTGTTTTTGGGATGG+GGG - chr2_1:74589789-74589808 Msa0224690:intron 40.0%
ACAAGATGATACCGTTGTTC+CGG - chr2_1:74589817-74589836 Msa0224690:intron 40.0%
ACAAAGTCTCTTCCATAAGG+AGG + chr2_1:74589920-74589939 None:intergenic 40.0%
TTTGTTAGCCATAAACCCAC+TGG - chr2_1:74589935-74589954 Msa0224690:CDS 40.0%
TTGTTAGCCATAAACCCACT+GGG - chr2_1:74589936-74589955 Msa0224690:CDS 40.0%
TTCTCCATGGGTTTGAATCT+CGG - chr2_1:74590076-74590095 Msa0224690:intron 40.0%
!!! GGTTTTTTCCTGTTGCTTGA+GGG + chr2_1:74590225-74590244 None:intergenic 40.0%
!!! AGGTTTTTTCCTGTTGCTTG+AGG + chr2_1:74590226-74590245 None:intergenic 40.0%
! AATTTTGCTTCAGCTGCATC+TGG - chr2_1:74590259-74590278 Msa0224690:CDS 40.0%
CTACCTTGGAAAGCTTACTT+TGG + chr2_1:74590430-74590449 None:intergenic 40.0%
! CTTTTTACTTCCAGCTACCT+TGG + chr2_1:74590444-74590463 None:intergenic 40.0%
CAAGGTAGCTGGAAGTAAAA+AGG - chr2_1:74590442-74590461 Msa0224690:five_prime_UTR 40.0%
! AAAACCTCCCAGTGGGTTTA+TGG + chr2_1:74589946-74589965 None:intergenic 45.0%
TATCTTAGCCCTCAAGCAAC+AGG - chr2_1:74590214-74590233 Msa0224690:CDS 45.0%
AGTAAGCTTTCCAAGGTAGC+TGG - chr2_1:74590431-74590450 Msa0224690:five_prime_UTR 45.0%
GGTGCATTGTACCTATTGAG+TGG - chr2_1:74590482-74590501 Msa0224690:five_prime_UTR 45.0%
GCATTGTACCTATTGAGTGG+TGG - chr2_1:74590485-74590504 Msa0224690:five_prime_UTR 45.0%
! AAAACCTCCCAGTGGGTTTA+TGG + chr2_1:74589946-74589965 None:intergenic 45.0%
TATCTTAGCCCTCAAGCAAC+AGG - chr2_1:74590214-74590233 Msa0224690:CDS 45.0%
AGTAAGCTTTCCAAGGTAGC+TGG - chr2_1:74590431-74590450 Msa0224690:five_prime_UTR 45.0%
GGTGCATTGTACCTATTGAG+TGG - chr2_1:74590482-74590501 Msa0224690:five_prime_UTR 45.0%
GCATTGTACCTATTGAGTGG+TGG - chr2_1:74590485-74590504 Msa0224690:five_prime_UTR 45.0%
!!! TTGTAATTTTTTTTTTTAAT+AGG - chr2_1:74590038-74590057 Msa0224690:intron 5.0%
!!! TTGTAATTTTTTTTTTTAAT+AGG - chr2_1:74590038-74590057 Msa0224690:intron 5.0%
TCACCAAACGTCACGATTGC+CGG + chr2_1:74589839-74589858 None:intergenic 50.0%
TTAGCCATAAACCCACTGGG+AGG - chr2_1:74589939-74589958 Msa0224690:CDS 50.0%
CCATTGCAAAACCTCCCAGT+GGG + chr2_1:74589953-74589972 None:intergenic 50.0%
ATGGCCGAGATTCAAACCCA+TGG + chr2_1:74590083-74590102 None:intergenic 50.0%
GCTTGAGGGCTAAGATATGC+AGG + chr2_1:74590211-74590230 None:intergenic 50.0%
TCACCAAACGTCACGATTGC+CGG + chr2_1:74589839-74589858 None:intergenic 50.0%
TTAGCCATAAACCCACTGGG+AGG - chr2_1:74589939-74589958 Msa0224690:CDS 50.0%
CCATTGCAAAACCTCCCAGT+GGG + chr2_1:74589953-74589972 None:intergenic 50.0%
ATGGCCGAGATTCAAACCCA+TGG + chr2_1:74590083-74590102 None:intergenic 50.0%
GCTTGAGGGCTAAGATATGC+AGG + chr2_1:74590211-74590230 None:intergenic 50.0%
CGTCACGATTGCCGGAACAA+CGG + chr2_1:74589831-74589850 None:intergenic 55.0%
GTTCCGGCAATCGTGACGTT+TGG - chr2_1:74589833-74589852 Msa0224690:intron 55.0%
! GACGTTTGGTGACTCTGCTG+TGG - chr2_1:74589847-74589866 Msa0224690:intron 55.0%
TGGTGACTCTGCTGTGGATG+TGG - chr2_1:74589853-74589872 Msa0224690:intron 55.0%
GGTGACTCTGCTGTGGATGT+GGG - chr2_1:74589854-74589873 Msa0224690:intron 55.0%
GTGACTCTGCTGTGGATGTG+GGG - chr2_1:74589855-74589874 Msa0224690:intron 55.0%
! CCCACTGGGAGGTTTTGCAA+TGG - chr2_1:74589950-74589969 Msa0224690:CDS 55.0%
GCCATTGCAAAACCTCCCAG+TGG + chr2_1:74589954-74589973 None:intergenic 55.0%
CGTCACGATTGCCGGAACAA+CGG + chr2_1:74589831-74589850 None:intergenic 55.0%
GTTCCGGCAATCGTGACGTT+TGG - chr2_1:74589833-74589852 Msa0224690:intron 55.0%
! GACGTTTGGTGACTCTGCTG+TGG - chr2_1:74589847-74589866 Msa0224690:intron 55.0%
TGGTGACTCTGCTGTGGATG+TGG - chr2_1:74589853-74589872 Msa0224690:intron 55.0%
GGTGACTCTGCTGTGGATGT+GGG - chr2_1:74589854-74589873 Msa0224690:intron 55.0%
GTGACTCTGCTGTGGATGTG+GGG - chr2_1:74589855-74589874 Msa0224690:intron 55.0%
! CCCACTGGGAGGTTTTGCAA+TGG - chr2_1:74589950-74589969 Msa0224690:CDS 55.0%
GCCATTGCAAAACCTCCCAG+TGG + chr2_1:74589954-74589973 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 74589655 74590514 74589655 ID=Msa0224690;Name=Msa0224690
chr2_1 mRNA 74589655 74590514 74589655 ID=Msa0224690-mRNA-1;Parent=Msa0224690;Name=Msa0224690-mRNA-1;_AED=0.03;_eAED=0.03;_QI=94|1|1|1|1|1|3|0|170
chr2_1 exon 74589655 74589786 74589655 ID=Msa0224690-mRNA-1:exon:22167;Parent=Msa0224690-mRNA-1
chr2_1 exon 74589860 74589990 74589860 ID=Msa0224690-mRNA-1:exon:22166;Parent=Msa0224690-mRNA-1
chr2_1 exon 74590174 74590514 74590174 ID=Msa0224690-mRNA-1:exon:22165;Parent=Msa0224690-mRNA-1
chr2_1 five_prime_UTR 74590421 74590514 74590421 ID=Msa0224690-mRNA-1:five_prime_utr;Parent=Msa0224690-mRNA-1
chr2_1 CDS 74590174 74590420 74590174 ID=Msa0224690-mRNA-1:cds;Parent=Msa0224690-mRNA-1
chr2_1 CDS 74589860 74589990 74589860 ID=Msa0224690-mRNA-1:cds;Parent=Msa0224690-mRNA-1
chr2_1 CDS 74589655 74589786 74589655 ID=Msa0224690-mRNA-1:cds;Parent=Msa0224690-mRNA-1
Gene Sequence

>Msa0224690

ATGAAGATGAATATTAGAGAAGCATTTTTTGTTGTGTTTGCATTTGTGTTTTTGGGATGGGGGAATGCACAAGATGATACCGTTGTTCCGGCAATCGTGACGTTTGGTGACTCTGCTGTGGATGTGGGGAACAATGACTATCTTTTCACTCTTTTTAAAGCTAACTATCCTCCTTATGGAAGAGACTTTGTTAGCCATAAACCCACTGGGAGGTTTTGCAATGGCAAATTAGCTACTGATATTACTGCTGAAACACTGGGTTTTAAGAGTTATGCACCTGCATATCTTAGCCCTCAAGCAACAGGAAAAAACCTTCTTATTGGAGCAAATTTTGCTTCAGCTGCATCTGGTTATGATGAAAAGGCTGCTATCTTGAATCACGCAATTCCATTGTCGCATCAGTTAAAATATTACAAGGAATACCAAAGTAAGCTTTCCAAGGTAGCTGGAAGTAAAAAGGCAGCATCAATTATTAAAGGTGCATTGTACCTATTGAGTGGTGGAAGTAGT
Protein sequence

>Msa0224690

MKMNIREAFFVVFAFVFLGWGNAQDDTVVPAIVTFGDSAVDVGNNDYLFTLFKANYPPYGRDFVSHKPTGRFCNGKLATDITAETLGFKSYAPAYLSPQATGKNLLIGANFASAASGYDEKAAILNHAIPLSHQLKYYKEYQSKLSKVAGSKKAASIIKGALYLLSGGSS