AlfalfaGEDB | Msa1293050

M. sativa cultivar zhongmu-4 / Msa1293050

KEGG: -
GO: -
PFAM: PF09713.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293050	XP_024629673.1	88.983	118	11	1	1	116	1	118	7.55e-69	215
Msa1293050	XP_024629673.1	91.304	69	6	0	107	175	46	114	1.11e-34	128

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293050	A0A396GKL3	91.304	69	6	0	107	175	46	114	3.71e-35	128
Msa1293050	A0A396GKL3	92.105	114	7	1	1	112	1	114	5.13e-69	214

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000780	Msa1293050	0.847396	1.187599e-59	-8.615850e-47
Msa0001460	Msa1293050	0.802876	4.556346e-49	-8.615850e-47
Msa0003470	Msa1293050	0.822406	2.409302e-53	-8.615850e-47
Msa0012460	Msa1293050	0.840064	1.088814e-57	-8.615850e-47
Msa0014410	Msa1293050	0.843519	1.332999e-58	-8.615850e-47
Msa0017120	Msa1293050	0.828702	7.763112e-55	-8.615850e-47
Msa0020630	Msa1293050	0.855926	4.543096e-62	-8.615850e-47
Msa0027540	Msa1293050	0.810988	8.763827e-51	-8.615850e-47
Msa0030730	Msa1293050	0.815819	7.602265e-52	-8.615850e-47
Msa0037820	Msa1293050	0.814969	1.175126e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293050	MtrunA17_Chr8g0368511	92.105	114	7	1	1	112	1	114	9.88e-73	214
Msa1293050	MtrunA17_Chr8g0368511	91.304	69	6	0	107	175	46	114	7.15e-39	128
Msa1293050	MtrunA17_Chr7g0222601	59.649	57	23	0	47	103	15	71	4.68e-22	85.5
Msa1293050	MtrunA17_Chr7g0222601	58.491	53	22	0	114	166	19	71	1.02e-19	79.3
Msa1293050	MtrunA17_Chr7g0263161	45.783	83	44	1	46	128	7	88	1.10e-21	84.0
Msa1293050	MtrunA17_Chr7g0263161	47.945	73	35	1	106	175	1	73	4.80e-19	77.4
Msa1293050	MtrunA17_Chr1g0212831	57.143	56	24	0	46	101	9	64	1.77e-19	79.0
Msa1293050	MtrunA17_Chr1g0212831	54.237	59	24	1	109	164	6	64	2.74e-17	73.2
Msa1293050	MtrunA17_Chr8g0347131	50.000	54	27	0	48	101	55	108	2.20e-12	63.9
Msa1293050	MtrunA17_Chr8g0347131	50.980	51	25	0	114	164	58	108	1.48e-11	61.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293050	AT1G10657.2	55.172	87	38	1	25	111	1	86	2.57e-30	107
Msa1293050	AT1G10657.2	52.941	68	31	1	107	174	20	86	8.52e-20	80.1
Msa1293050	AT1G10657.3	55.172	87	38	1	25	111	1	86	2.69e-30	106
Msa1293050	AT1G10657.3	52.941	68	31	1	107	174	20	86	9.34e-20	79.7
Msa1293050	AT1G10657.1	43.243	111	38	2	25	111	1	110	8.87e-25	93.6
Msa1293050	AT1G10657.1	39.130	92	31	2	107	174	20	110	2.89e-14	66.2
Msa1293050	AT1G10657.4	43.243	111	38	2	25	111	1	110	1.03e-24	93.2
Msa1293050	AT1G10657.4	39.130	92	31	2	107	174	20	110	3.53e-14	65.9
Msa1293050	AT3G55240.1	62.500	56	21	0	46	101	8	63	3.33e-23	88.6
Msa1293050	AT3G55240.1	55.738	61	24	1	107	164	3	63	1.27e-20	82.0

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Find 52 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTAAAGTTGTACCATA+TGG	0.440254	8_3:-49949731	Msa1293050:three_prime_UTR
AAATAGCTCATGAAATCCAA+AGG	0.624632	8_3:+49953504	None:intergenic
AAATGATCAACCAAGTGAGA+TGG	0.602265	8_3:-49949980	Msa1293050:three_prime_UTR
AAGGCACTAGAAGAACATGC+AGG	0.583674	8_3:-49950509	Msa1293050:CDS
AAGGCACTAGAAGAACATGC+AGG	0.583674	8_3:-49953036	Msa1293050:CDS
ACAAACAAGGAAGAGGGTAT+TGG	0.476829	8_3:+49953289	None:intergenic
ACAACCAATGCCGTGCCATA+TGG	0.455124	8_3:+49949716	None:intergenic
ACCATTCTAATATAGCTATG+TGG	0.637830	8_3:+49953258	None:intergenic
ACGGCATTGGTTGTAACTTT+TGG	0.279024	8_3:-49949707	Msa1293050:three_prime_UTR
ACGTACGTGAACGAGCTTGG+AGG	0.547000	8_3:+49950303	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAGCTCATGAAATCCAA+AGG	+	chr8_3:49949705-49949724	None:intergenic	30.0%
TCCACATAGCTATATTAGAA+TGG	-	chr8_3:49949947-49949966	Msa1293050:three_prime_UTR	30.0%
ACCATTCTAATATAGCTATG+TGG	+	chr8_3:49949951-49949970	None:intergenic	30.0%
TTTCATGCATGGAAAAATGT+AGG	-	chr8_3:49950091-49950110	Msa1293050:intron	30.0%
AAATGATCAACAAAGTGAGA+TGG	-	chr8_3:49950765-49950784	Msa1293050:intron	30.0%
ATTATGGTCACAAAGTCTAA+TGG	+	chr8_3:49950875-49950894	None:intergenic	30.0%
ATTGTAAATGAAGCTGTCAT+TGG	-	chr8_3:49951125-49951144	Msa1293050:intron	30.0%
GTAATAAAGTGATAGGTGTT+AGG	+	chr8_3:49951513-49951532	None:intergenic	30.0%
TAGAAGGAATTGGAAAGTTA+TGG	+	chr8_3:49951563-49951582	None:intergenic	30.0%
GAAGGAAAAAGTTCAATAGA+AGG	+	chr8_3:49951579-49951598	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	49949691	49953537	49949691	ID=Msa1293050;Name=Msa1293050
chr8_3	mRNA	49949691	49953537	49949691	ID=Msa1293050-mRNA-1;Parent=Msa1293050;Name=Msa1293050-mRNA-1;_AED=0.21;_eAED=0.21;_QI=128\|0.8\|0.83\|1\|0.2\|0.33\|6\|334\|175
chr8_3	exon	49949691	49950028	49949691	ID=Msa1293050-mRNA-1:exon:13907;Parent=Msa1293050-mRNA-1
chr8_3	exon	49950309	49950387	49950309	ID=Msa1293050-mRNA-1:exon:13906;Parent=Msa1293050-mRNA-1
chr8_3	exon	49950483	49950588	49950483	ID=Msa1293050-mRNA-1:exon:13905;Parent=Msa1293050-mRNA-1
chr8_3	exon	49952830	49952912	49952830	ID=Msa1293050-mRNA-1:exon:13904;Parent=Msa1293050-mRNA-1
chr8_3	exon	49953010	49953115	49953010	ID=Msa1293050-mRNA-1:exon:13903;Parent=Msa1293050-mRNA-1
chr8_3	exon	49953260	49953537	49953260	ID=Msa1293050-mRNA-1:exon:13902;Parent=Msa1293050-mRNA-1
chr8_3	five_prime_UTR	49953410	49953537	49953410	ID=Msa1293050-mRNA-1:five_prime_utr;Parent=Msa1293050-mRNA-1
chr8_3	CDS	49953260	49953409	49953260	ID=Msa1293050-mRNA-1:cds;Parent=Msa1293050-mRNA-1
chr8_3	CDS	49953010	49953115	49953010	ID=Msa1293050-mRNA-1:cds;Parent=Msa1293050-mRNA-1
chr8_3	CDS	49952830	49952912	49952830	ID=Msa1293050-mRNA-1:cds;Parent=Msa1293050-mRNA-1
chr8_3	CDS	49950483	49950588	49950483	ID=Msa1293050-mRNA-1:cds;Parent=Msa1293050-mRNA-1
chr8_3	CDS	49950309	49950387	49950309	ID=Msa1293050-mRNA-1:cds;Parent=Msa1293050-mRNA-1
chr8_3	CDS	49950025	49950028	49950025	ID=Msa1293050-mRNA-1:cds;Parent=Msa1293050-mRNA-1
chr8_3	three_prime_UTR	49949691	49950024	49949691	ID=Msa1293050-mRNA-1:three_prime_utr;Parent=Msa1293050-mRNA-1

Gene Sequence

>Msa1293050

ATGTACTTTTTCTCTGTGTGCTTTCTTCTATTTCTTTCTATATTCTATTTGTTTTGTCCATTAGCAACAGAATTAAGCACAATGCATGATCACACTCACCAATACCCTCTTCCTTGTTTGTATTGTCATCCACATAGCTATATTAGAATGGTTCAAAACCTGATAGAGAGATGCATGATCTTTCACATGAGTCAAGACCAATGCATAAAGGCACTAGAAGAACATGCAGGGATTCAGCCACTTGTTACTCTTACAGTGTGGAGAGAGTTGCAAAAGGAGAATGAAGAGTTCTTTAGAGCATATTTGCAACAATTTATCCCTCCAAGCTCGTTCACGTACGTTCAAAATCTCATAGAGAGATGCATGATCTTTCACATGAGTCAAGACCAATGCATAAAGGCACTAGAAGAACATGCAGGGATTCAGCCACTTGTTACTCTTACAGTGTGGAGGGAGTTGCAAAAGGAGAATGAAGAGTTCTTTAGAGCATATTTGCAACAATTTATCCCTCCAAGCTCGTTCACGTAG

Protein sequence

>Msa1293050

MYFFSVCFLLFLSIFYLFCPLATELSTMHDHTHQYPLPCLYCHPHSYIRMVQNLIERCMIFHMSQDQCIKALEEHAGIQPLVTLTVWRELQKENEEFFRAYLQQFIPPSSFTYVQNLIERCMIFHMSQDQCIKALEEHAGIQPLVTLTVWRELQKENEEFFRAYLQQFIPPSSFT*