AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33047


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33047.t1 MTR_1g015290 97.600 125 3 0 1 125 1 125 7.43e-83 238
MS.gene33047.t1 MTR_3g104950 64.516 124 39 2 1 124 1 119 3.17e-47 148
MS.gene33047.t1 MTR_4g125600 59.524 126 39 6 1 124 1 116 4.40e-35 117
MS.gene33047.t1 MTR_2g041260 55.200 125 49 3 1 124 1 119 8.80e-23 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33047.t1 AT2G06520 56.604 106 44 2 20 125 13 116 2.16e-27 98.2

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGGGTGGCTTGGGTTAG+TGG 0.257951 1.1:+6560381 None:intergenic
TTTAACTAAGTTCTCTTAAC+AGG 0.285839 1.1:+6560051 None:intergenic
TGTTGTTCTTACAGCTATAT+TGG 0.300493 1.1:-6560103 MS.gene33047:CDS
GTTGTTCTTACAGCTATATT+GGG 0.346937 1.1:-6560102 MS.gene33047:CDS
GTCTTCTTTGCTTGTGGAAA+GGG 0.349407 1.1:+6560315 None:intergenic
GCTATATTGGGTGCTGTTGT+TGG 0.379016 1.1:-6560090 MS.gene33047:CDS
ACTTCAAAATCCAACCTTGT+TGG 0.385727 1.1:-6560276 MS.gene33047:CDS
ACTAGTGTCTTCTTTGCTTG+TGG 0.430477 1.1:+6560309 None:intergenic
CCAGATGTTGCTCATGCAGC+TGG 0.445643 1.1:-6560180 MS.gene33047:CDS
TGTCTTCTTTGCTTGTGGAA+AGG 0.459779 1.1:+6560314 None:intergenic
TTTGCTTGTGGAAAGGGCAA+TGG 0.463285 1.1:+6560321 None:intergenic
TCTCTTAACAGGATCAAAGT+TGG 0.467747 1.1:+6560062 None:intergenic
TGCTTGAATTCCAACAAGGT+TGG 0.477724 1.1:+6560266 None:intergenic
TTCAAGAAGGGTGTGGTGGA+AGG 0.483812 1.1:+6560345 None:intergenic
CCAGCTGCATGAGCAACATC+TGG 0.490868 1.1:+6560180 None:intergenic
TTGCTTAGCATTGCTGCTGG+TGG 0.565122 1.1:-6560126 MS.gene33047:CDS
ATTGAAAGACAAAACAGTGA+AGG 0.569072 1.1:-6560241 MS.gene33047:CDS
TTGAAAGACAAAACAGTGAA+GGG 0.580339 1.1:-6560240 MS.gene33047:CDS
AAGGGCAATGGTTTCAAGAA+GGG 0.584655 1.1:+6560333 None:intergenic
AAAGGGCAATGGTTTCAAGA+AGG 0.598373 1.1:+6560332 None:intergenic
CAATGGTTTCAAGAAGGGTG+TGG 0.606722 1.1:+6560338 None:intergenic
TGGTTTCAAGAAGGGTGTGG+TGG 0.610036 1.1:+6560341 None:intergenic
GTGTGGTGGAAGGATTGTGA+AGG 0.617280 1.1:+6560355 None:intergenic
AACATCTGGTAACACCATAG+AGG 0.635135 1.1:+6560194 None:intergenic
ATGATGCTTGAATTCCAACA+AGG 0.644146 1.1:+6560262 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCAAAAAGTTTTTAAGAGA+TGG + chr1.1:6560326-6560345 None:intergenic 25.0%
ATTGAAAGACAAAACAGTGA+AGG - chr1.1:6560226-6560245 MS.gene33047:CDS 30.0%
GTTGTTCTTACAGCTATATT+GGG - chr1.1:6560365-6560384 MS.gene33047:CDS 30.0%
TGTTGTTCTTACAGCTATAT+TGG - chr1.1:6560364-6560383 MS.gene33047:CDS 30.0%
TTGAAAGACAAAACAGTGAA+GGG - chr1.1:6560227-6560246 MS.gene33047:CDS 30.0%
ACTTCAAAATCCAACCTTGT+TGG - chr1.1:6560191-6560210 MS.gene33047:CDS 35.0%
ATGATGCTTGAATTCCAACA+AGG + chr1.1:6560208-6560227 None:intergenic 35.0%
TCTCTTAACAGGATCAAAGT+TGG + chr1.1:6560408-6560427 None:intergenic 35.0%
! AAGGATTGTGAAGGTTTTTG+TGG + chr1.1:6560106-6560125 None:intergenic 35.0%
! AGGATTGTGAAGGTTTTTGT+GGG + chr1.1:6560105-6560124 None:intergenic 35.0%
! TTTTGCATTAACAGCCTCTA+TGG - chr1.1:6560259-6560278 MS.gene33047:CDS 35.0%
AAAGGGCAATGGTTTCAAGA+AGG + chr1.1:6560138-6560157 None:intergenic 40.0%
AACATCTGGTAACACCATAG+AGG + chr1.1:6560276-6560295 None:intergenic 40.0%
AAGGGCAATGGTTTCAAGAA+GGG + chr1.1:6560137-6560156 None:intergenic 40.0%
ACTAGTGTCTTCTTTGCTTG+TGG + chr1.1:6560161-6560180 None:intergenic 40.0%
GTCTTCTTTGCTTGTGGAAA+GGG + chr1.1:6560155-6560174 None:intergenic 40.0%
TGCTTGAATTCCAACAAGGT+TGG + chr1.1:6560204-6560223 None:intergenic 40.0%
TGTCTTCTTTGCTTGTGGAA+AGG + chr1.1:6560156-6560175 None:intergenic 40.0%
! ATTGTGAAGGTTTTTGTGGG+TGG + chr1.1:6560102-6560121 None:intergenic 40.0%
! TTTTTGCTTAGCATTGCTGC+TGG - chr1.1:6560338-6560357 MS.gene33047:CDS 40.0%
CAATGGTTTCAAGAAGGGTG+TGG + chr1.1:6560132-6560151 None:intergenic 45.0%
TTTGCTTGTGGAAAGGGCAA+TGG + chr1.1:6560149-6560168 None:intergenic 45.0%
! AAGGTTTTTGTGGGTGGCTT+GGG + chr1.1:6560096-6560115 None:intergenic 45.0%
!! GCTATATTGGGTGCTGTTGT+TGG - chr1.1:6560377-6560396 MS.gene33047:CDS 45.0%
TTGCTTAGCATTGCTGCTGG+TGG - chr1.1:6560341-6560360 MS.gene33047:CDS 50.0%
! GAAGGTTTTTGTGGGTGGCT+TGG + chr1.1:6560097-6560116 None:intergenic 50.0%
! GTGTGGTGGAAGGATTGTGA+AGG + chr1.1:6560115-6560134 None:intergenic 50.0%
! TGGTTTCAAGAAGGGTGTGG+TGG + chr1.1:6560129-6560148 None:intergenic 50.0%
! TTCAAGAAGGGTGTGGTGGA+AGG + chr1.1:6560125-6560144 None:intergenic 50.0%
CCAGATGTTGCTCATGCAGC+TGG - chr1.1:6560287-6560306 MS.gene33047:CDS 55.0%
CCAGCTGCATGAGCAACATC+TGG + chr1.1:6560290-6560309 None:intergenic 55.0%
! TTGTGGGTGGCTTGGGTTAG+TGG + chr1.1:6560089-6560108 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 6560056 6560433 6560056 ID=MS.gene33047
chr1.1 mRNA 6560056 6560433 6560056 ID=MS.gene33047.t1;Parent=MS.gene33047
chr1.1 exon 6560056 6560433 6560056 ID=MS.gene33047.t1.exon1;Parent=MS.gene33047.t1
chr1.1 CDS 6560056 6560433 6560056 ID=cds.MS.gene33047.t1;Parent=MS.gene33047.t1
Gene Sequence

>MS.gene33047

ATGGCTTCAGCTTCTGTTTCAATGTCTTTACCACTAACCCAAGCCACCCACAAAAACCTTCACAATCCTTCCACCACACCCTTCTTGAAACCATTGCCCTTTCCACAAGCAAAGAAGACACTAGTCAACAAGTCAACTTCAAAATCCAACCTTGTTGGAATTCAAGCATCATTGAAAGACAAAACAGTGAAGGGTCTAACAGCTTTTGCATTAACAGCCTCTATGGTGTTACCAGATGTTGCTCATGCAGCTGGAAATGATTTTTCACCATCTCTTAAAAACTTTTTGCTTAGCATTGCTGCTGGTGGTGTTGTTCTTACAGCTATATTGGGTGCTGTTGTTGGTGTTTCCAACTTTGATCCTGTTAAGAGAACTTAG

Protein sequence

>MS.gene33047.t1

MASASVSMSLPLTQATHKNLHNPSTTPFLKPLPFPQAKKTLVNKSTSKSNLVGIQASLKDKTVKGLTAFALTASMVLPDVAHAAGNDFSPSLKNFLLSIAAGGVVLTAILGAVVGVSNFDPVKRT