AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22667


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22667.t1 MTR_7g114900 100.000 203 0 0 12 214 74 276 3.71e-147 410
MS.gene22667.t1 MTR_1g108440 66.010 203 68 1 12 213 61 263 6.70e-93 273
MS.gene22667.t1 MTR_2g032540 25.234 214 145 5 12 213 73 283 2.47e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22667.t1 AT3G04790 78.325 203 44 0 12 214 70 272 1.87e-116 333
MS.gene22667.t1 AT2G01290 61.084 203 79 0 12 214 58 260 7.03e-87 258
MS.gene22667.t1 AT1G71100 59.903 207 78 1 12 213 54 260 1.11e-80 242
MS.gene22667.t1 AT5G44520 30.556 216 134 5 12 214 62 274 7.12e-22 91.3
MS.gene22667.t1 AT5G44520 30.556 216 134 5 12 214 92 304 1.23e-21 91.3

Find 62 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTTCTCAACCTTGTTAA+AGG 0.149369 7.2:+2402701 MS.gene22667:CDS
GTTGAGAAGTTCGCCGAGTT+TGG 0.275002 7.2:-2402537 None:intergenic
TGGTATTCCTCTCTCTGTTC+TGG 0.303086 7.2:+2402628 MS.gene22667:CDS
AGCTTGTTCTTCGGTGCGTT+TGG 0.324267 7.2:-2402597 None:intergenic
TGCTCTCCTCCGTGAGAAAA+TGG 0.332089 7.2:+2402733 MS.gene22667:CDS
GTTGGAAATATAATTTGATT+AGG 0.342353 7.2:+2402861 MS.gene22667:CDS
GTCATCATTGCTGGTAAAAC+TGG 0.347429 7.2:+2403088 MS.gene22667:CDS
AGCAGCATTTGCATCTCTAA+TGG 0.354451 7.2:-2402999 None:intergenic
TGGTCCTCGGCCTCGGCAAC+CGG 0.371558 7.2:+2402491 MS.gene22667:CDS
AGCGGCATGGTCCTCGGCCT+CGG 0.401792 7.2:+2402484 MS.gene22667:CDS
TTCTTCGGTGCGTTTGGAAG+TGG 0.409826 7.2:-2402591 None:intergenic
GCTACCTCTGTCATCATTGC+TGG 0.413073 7.2:+2403079 MS.gene22667:CDS
GCTGAGATTTCGTCGCTTGA+AGG 0.433006 7.2:+2403025 MS.gene22667:CDS
TTAGAATTATCAGTAACATA+TGG 0.433220 7.2:-2402947 None:intergenic
GACGAAGGCGGCGGTGGAGC+CGG 0.434905 7.2:-2402510 None:intergenic
GCAGCATTTGCATCTCTAAT+GGG 0.454829 7.2:-2402998 None:intergenic
GTCTCTGGTCTCGGTGGAAG+CGG 0.462851 7.2:+2402806 MS.gene22667:CDS
ACGAAACTGGTCTCTGGTCT+CGG 0.463473 7.2:+2402797 MS.gene22667:CDS
CTCAACCTTGTTAAAGGCCG+CGG 0.475097 7.2:+2402707 MS.gene22667:CDS
CCTTTAACAAGGTTGAGAAA+TGG 0.475449 7.2:-2402701 None:intergenic
GGTGGTCGATGATACGAAAC+TGG 0.476820 7.2:+2402784 MS.gene22667:CDS
GGAGACGACGAAGGCGGCGG+TGG 0.479468 7.2:-2402516 None:intergenic
ATTAGAGATGCAAATGCTGC+TGG 0.490372 7.2:+2403001 MS.gene22667:CDS
CAAGAATTGTTCAAAGAAGA+AGG 0.494177 7.2:+2402887 MS.gene22667:CDS
GAAGAACAAGCTCGTTCTCT+TGG 0.494473 7.2:+2402608 MS.gene22667:CDS
GTCAAAAGCGGCATGGTCCT+CGG 0.496677 7.2:+2402478 MS.gene22667:CDS
CGGTTTAGCGATGCCGGTGG+AGG 0.498139 7.2:+2402826 MS.gene22667:CDS
AAGAGAACGAGCTTGTTCTT+CGG 0.503417 7.2:-2402606 None:intergenic
CTTGAAGGTGTCGTCGAGCA+TGG 0.504176 7.2:+2403040 MS.gene22667:CDS
GGAGCCGGTTGCCGAGGCCG+AGG 0.504214 7.2:-2402495 None:intergenic
TCTTCGGTGCGTTTGGAAGT+GGG 0.512477 7.2:-2402590 None:intergenic
TGGAAGCGGTTTAGCGATGC+CGG 0.518745 7.2:+2402820 MS.gene22667:CDS
GATGATACGAAACTGGTCTC+TGG 0.522213 7.2:+2402791 MS.gene22667:CDS
CACCGCCGCGGCCTTTAACA+AGG 0.522675 7.2:-2402712 None:intergenic
GCATGGTTTGTTCTTGAACA+TGG 0.523331 7.2:+2403057 MS.gene22667:CDS
CGTCTCGATCTCGCCATCGA+CGG 0.527285 7.2:+2402662 MS.gene22667:CDS
GGCGGTGGAGCCGGTTGCCG+AGG 0.528429 7.2:-2402501 None:intergenic
GATGATGTTGGTGAGTTCGC+CGG 0.539899 7.2:-2402561 None:intergenic
GCCTTCGTCGTCTCCAAACT+CGG 0.546205 7.2:+2402524 MS.gene22667:CDS
GGCGAACTCACCAACATCAT+CGG 0.560538 7.2:+2402563 MS.gene22667:CDS
CAAAACTGAACAACCTCCAC+CGG 0.562915 7.2:-2402839 None:intergenic
TTTGGAGACGACGAAGGCGG+CGG 0.566766 7.2:-2402519 None:intergenic
GCCGTTGAATATGTCAAAAG+CGG 0.580001 7.2:+2402466 None:intergenic
CACGACGAATTTATCAGAAG+CGG 0.584428 7.2:-2402762 None:intergenic
TTATCGTCCAGAACAGAGAG+AGG 0.606235 7.2:-2402635 None:intergenic
TTCTGATAAATTCGTCGTGG+TGG 0.606783 7.2:+2402766 MS.gene22667:CDS
GACTTCATCAGCACCGTCGA+TGG 0.609689 7.2:-2402675 None:intergenic
AGTGGGAACGCCGATGATGT+TGG 0.637767 7.2:-2402573 None:intergenic
AAGCGGTTTAGCGATGCCGG+TGG 0.640481 7.2:+2402823 MS.gene22667:CDS
GCCGAGTTTGGAGACGACGA+AGG 0.645857 7.2:-2402525 None:intergenic
CGTTGATGCGAAATTGAGAG+TGG 0.649594 7.2:+2402910 MS.gene22667:CDS
AAATTGAGAGTGGATGAAAG+TGG 0.649838 7.2:+2402920 MS.gene22667:CDS
TGAATATGTCAAAAGCGGCA+TGG 0.650597 7.2:+2402471 None:intergenic
GAGTTTGGAGACGACGAAGG+CGG 0.656291 7.2:-2402522 None:intergenic
CTCGGCGAACTTCTCAACTC+CGG 0.656946 7.2:+2402542 MS.gene22667:CDS
CGCTTCTGATAAATTCGTCG+TGG 0.657924 7.2:+2402763 MS.gene22667:CDS
AACCTTGTTAAAGGCCGCGG+CGG 0.661052 7.2:+2402710 MS.gene22667:CDS
CACGGAGGAGAGCACCGCCG+CGG 0.668975 7.2:-2402724 None:intergenic
AAACTGGTCTCTGGTCTCGG+TGG 0.677817 7.2:+2402800 MS.gene22667:CDS
TTTACCAGCAATGATGACAG+AGG 0.680041 7.2:-2403083 None:intergenic
TCTCCTCCGTGAGAAAATGG+TGG 0.693512 7.2:+2402736 MS.gene22667:CDS
TCGATGGCGAGATCGAGACG+AGG 0.700409 7.2:-2402659 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTGGAAATATAATTTGATT+AGG + chr7.2:2402861-2402880 MS.gene22667:CDS 20.0%
!! TTAGAATTATCAGTAACATA+TGG - chr7.2:2402950-2402969 None:intergenic 20.0%
CAAGAATTGTTCAAAGAAGA+AGG + chr7.2:2402887-2402906 MS.gene22667:CDS 30.0%
AAATTGAGAGTGGATGAAAG+TGG + chr7.2:2402920-2402939 MS.gene22667:CDS 35.0%
CCATTTCTCAACCTTGTTAA+AGG + chr7.2:2402701-2402720 MS.gene22667:CDS 35.0%
CCTTTAACAAGGTTGAGAAA+TGG - chr7.2:2402704-2402723 None:intergenic 35.0%
AGCAGCATTTGCATCTCTAA+TGG - chr7.2:2403002-2403021 None:intergenic 40.0%
ATTAGAGATGCAAATGCTGC+TGG + chr7.2:2403001-2403020 MS.gene22667:CDS 40.0%
CACGACGAATTTATCAGAAG+CGG - chr7.2:2402765-2402784 None:intergenic 40.0%
GCAGCATTTGCATCTCTAAT+GGG - chr7.2:2403001-2403020 None:intergenic 40.0%
GTCATCATTGCTGGTAAAAC+TGG + chr7.2:2403088-2403107 MS.gene22667:CDS 40.0%
TTCTGATAAATTCGTCGTGG+TGG + chr7.2:2402766-2402785 MS.gene22667:CDS 40.0%
TTTACCAGCAATGATGACAG+AGG - chr7.2:2403086-2403105 None:intergenic 40.0%
! AAGAGAACGAGCTTGTTCTT+CGG - chr7.2:2402609-2402628 None:intergenic 40.0%
!! GCATGGTTTGTTCTTGAACA+TGG + chr7.2:2403057-2403076 MS.gene22667:CDS 40.0%
!! TGGAGGTTGTTCAGTTTTGT+TGG + chr7.2:2402843-2402862 MS.gene22667:CDS 40.0%
CAAAACTGAACAACCTCCAC+CGG - chr7.2:2402842-2402861 None:intergenic 45.0%
CGCTTCTGATAAATTCGTCG+TGG + chr7.2:2402763-2402782 MS.gene22667:CDS 45.0%
CGTTGATGCGAAATTGAGAG+TGG + chr7.2:2402910-2402929 MS.gene22667:CDS 45.0%
GAAGAACAAGCTCGTTCTCT+TGG + chr7.2:2402608-2402627 MS.gene22667:CDS 45.0%
TGGTATTCCTCTCTCTGTTC+TGG + chr7.2:2402628-2402647 MS.gene22667:CDS 45.0%
TTATCGTCCAGAACAGAGAG+AGG - chr7.2:2402638-2402657 None:intergenic 45.0%
! GATGATACGAAACTGGTCTC+TGG + chr7.2:2402791-2402810 MS.gene22667:CDS 45.0%
CTCAACCTTGTTAAAGGCCG+CGG + chr7.2:2402707-2402726 MS.gene22667:CDS 50.0%
GCTACCTCTGTCATCATTGC+TGG + chr7.2:2403079-2403098 MS.gene22667:CDS 50.0%
GCTGAGATTTCGTCGCTTGA+AGG + chr7.2:2403025-2403044 MS.gene22667:CDS 50.0%
GGCGAACTCACCAACATCAT+CGG + chr7.2:2402563-2402582 MS.gene22667:CDS 50.0%
GGTGGTCGATGATACGAAAC+TGG + chr7.2:2402784-2402803 MS.gene22667:CDS 50.0%
GTTGAGAAGTTCGCCGAGTT+TGG - chr7.2:2402540-2402559 None:intergenic 50.0%
TCTCCTCCGTGAGAAAATGG+TGG + chr7.2:2402736-2402755 MS.gene22667:CDS 50.0%
TGCTCTCCTCCGTGAGAAAA+TGG + chr7.2:2402733-2402752 MS.gene22667:CDS 50.0%
! ACGAAACTGGTCTCTGGTCT+CGG + chr7.2:2402797-2402816 MS.gene22667:CDS 50.0%
! AGCTTGTTCTTCGGTGCGTT+TGG - chr7.2:2402600-2402619 None:intergenic 50.0%
! CGGCTTCCACCATTTTCTCA+CGG - chr7.2:2402745-2402764 None:intergenic 50.0%
! CTTCCACCATTTTCTCACGG+AGG - chr7.2:2402742-2402761 None:intergenic 50.0%
! GATGATGTTGGTGAGTTCGC+CGG - chr7.2:2402564-2402583 None:intergenic 50.0%
! TCTTCGGTGCGTTTGGAAGT+GGG - chr7.2:2402593-2402612 None:intergenic 50.0%
! TTCTTCGGTGCGTTTGGAAG+TGG - chr7.2:2402594-2402613 None:intergenic 50.0%
AACCTTGTTAAAGGCCGCGG+CGG + chr7.2:2402710-2402729 MS.gene22667:CDS 55.0%
AGTGGGAACGCCGATGATGT+TGG - chr7.2:2402576-2402595 None:intergenic 55.0%
CTCGGCGAACTTCTCAACTC+CGG + chr7.2:2402542-2402561 MS.gene22667:CDS 55.0%
GACTTCATCAGCACCGTCGA+TGG - chr7.2:2402678-2402697 None:intergenic 55.0%
GAGTTTGGAGACGACGAAGG+CGG - chr7.2:2402525-2402544 None:intergenic 55.0%
GCCTTCGTCGTCTCCAAACT+CGG + chr7.2:2402524-2402543 MS.gene22667:CDS 55.0%
GTCAAAAGCGGCATGGTCCT+CGG + chr7.2:2402478-2402497 MS.gene22667:CDS 55.0%
TGGAAGCGGTTTAGCGATGC+CGG + chr7.2:2402820-2402839 MS.gene22667:CDS 55.0%
! AAACTGGTCTCTGGTCTCGG+TGG + chr7.2:2402800-2402819 MS.gene22667:CDS 55.0%
!! CTTGAAGGTGTCGTCGAGCA+TGG + chr7.2:2403040-2403059 MS.gene22667:CDS 55.0%
AAGCGGTTTAGCGATGCCGG+TGG + chr7.2:2402823-2402842 MS.gene22667:CDS 60.0%
CGTCTCGATCTCGCCATCGA+CGG + chr7.2:2402662-2402681 MS.gene22667:CDS 60.0%
GCCGAGTTTGGAGACGACGA+AGG - chr7.2:2402528-2402547 None:intergenic 60.0%
GTCTCTGGTCTCGGTGGAAG+CGG + chr7.2:2402806-2402825 MS.gene22667:CDS 60.0%
TCGATGGCGAGATCGAGACG+AGG - chr7.2:2402662-2402681 None:intergenic 60.0%
TTTGGAGACGACGAAGGCGG+CGG - chr7.2:2402522-2402541 None:intergenic 60.0%
CACCGCCGCGGCCTTTAACA+AGG - chr7.2:2402715-2402734 None:intergenic 65.0%
CGGTTTAGCGATGCCGGTGG+AGG + chr7.2:2402826-2402845 MS.gene22667:CDS 65.0%
AGCGGCATGGTCCTCGGCCT+CGG + chr7.2:2402484-2402503 MS.gene22667:CDS 70.0%
TGGTCCTCGGCCTCGGCAAC+CGG + chr7.2:2402491-2402510 MS.gene22667:CDS 70.0%
GACGAAGGCGGCGGTGGAGC+CGG - chr7.2:2402513-2402532 None:intergenic 75.0%
GGAGACGACGAAGGCGGCGG+TGG - chr7.2:2402519-2402538 None:intergenic 75.0%
!! CACGGAGGAGAGCACCGCCG+CGG - chr7.2:2402727-2402746 None:intergenic 75.0%
! GGAGCCGGTTGCCGAGGCCG+AGG - chr7.2:2402498-2402517 None:intergenic 80.0%
! GGCGGTGGAGCCGGTTGCCG+AGG - chr7.2:2402504-2402523 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 2402476 2403117 2402476 ID=MS.gene22667
chr7.2 mRNA 2402476 2403117 2402476 ID=MS.gene22667.t1;Parent=MS.gene22667
chr7.2 exon 2402476 2403117 2402476 ID=MS.gene22667.t1.exon1;Parent=MS.gene22667.t1
chr7.2 CDS 2402476 2403117 2402476 ID=cds.MS.gene22667.t1;Parent=MS.gene22667.t1
Gene Sequence

>MS.gene22667

ATGTCAAAAGCGGCATGGTCCTCGGCCTCGGCAACCGGCTCCACCGCCGCCTTCGTCGTCTCCAAACTCGGCGAACTTCTCAACTCCGGCGAACTCACCAACATCATCGGCGTTCCCACTTCCAAACGCACCGAAGAACAAGCTCGTTCTCTTGGTATTCCTCTCTCTGTTCTGGACGATAATCCTCGTCTCGATCTCGCCATCGACGGTGCTGATGAAGTCGATCCATTTCTCAACCTTGTTAAAGGCCGCGGCGGTGCTCTCCTCCGTGAGAAAATGGTGGAAGCCGCTTCTGATAAATTCGTCGTGGTGGTCGATGATACGAAACTGGTCTCTGGTCTCGGTGGAAGCGGTTTAGCGATGCCGGTGGAGGTTGTTCAGTTTTGTTGGAAATATAATTTGATTAGGCTACAAGAATTGTTCAAAGAAGAAGGCGTTGATGCGAAATTGAGAGTGGATGAAAGTGGAAAACCATATGTTACTGATAATTCTAATTACATTGTTGATTTGTATTTTAAGACTCCCATTAGAGATGCAAATGCTGCTGGAGCTGAGATTTCGTCGCTTGAAGGTGTCGTCGAGCATGGTTTGTTCTTGAACATGGCTACCTCTGTCATCATTGCTGGTAAAACTGGTGTTGAA

Protein sequence

>MS.gene22667.t1

MSKAAWSSASATGSTAAFVVSKLGELLNSGELTNIIGVPTSKRTEEQARSLGIPLSVLDDNPRLDLAIDGADEVDPFLNLVKGRGGALLREKMVEAASDKFVVVVDDTKLVSGLGGSGLAMPVEVVQFCWKYNLIRLQELFKEEGVDAKLRVDESGKPYVTDNSNYIVDLYFKTPIRDANAAGAEISSLEGVVEHGLFLNMATSVIIAGKTGVE