AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36336


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36336.t1 MTR_2g101430 88.732 213 16 1 1 205 61 273 1.05e-131 371
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36336.t1 AT1G51080 45.894 207 101 4 1 199 77 280 1.04e-46 155

Find 64 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACCTTCTCGGCACTTCTA+AGG 0.214375 2.2:-2139197 MS.gene36336:CDS
TTTGCTCTTAATGTGTTAAA+TGG 0.235237 2.2:-2139340 MS.gene36336:CDS
GTTAGGTTGGGTGTTGGTAT+TGG 0.289295 2.2:+2139132 None:intergenic
TAGCAGGTGGGTGGTTAGGT+TGG 0.367914 2.2:+2139119 None:intergenic
AAAGTTGATTTCGACCTTCT+CGG 0.376013 2.2:-2139208 MS.gene36336:CDS
TAGGATGCTGCCATGGTTGA+TGG 0.394720 2.2:+2138886 None:intergenic
TCAATGCGGTCTTTGTAGAT+TGG 0.399152 2.2:+2139370 None:intergenic
CTTAAGAGCACGCGAGTTTG+AGG 0.411946 2.2:-2139428 MS.gene36336:CDS
CTAGTAGCAGGTGGGTGGTT+AGG 0.415512 2.2:+2139115 None:intergenic
CAATCCATTCCCCGGACGTC+AGG 0.418797 2.2:-2138924 MS.gene36336:CDS
TCTGCTGCTGCTAGATCCAT+TGG 0.419249 2.2:-2139304 MS.gene36336:CDS
CTTTGTAGATTGGGTCTAGA+AGG 0.419945 2.2:+2139380 None:intergenic
CAATGCGGTCTTTGTAGATT+GGG 0.420162 2.2:+2139371 None:intergenic
TTTAAAGTTGAGTTTAGCTT+AGG 0.423436 2.2:+2138867 None:intergenic
AGCAGGTGGGTGGTTAGGTT+GGG 0.433268 2.2:+2139120 None:intergenic
CAGGAGATAAGCAAGAGATT+TGG 0.434593 2.2:-2139262 MS.gene36336:CDS
TGGGTGGTTAGGTTGGGTGT+TGG 0.441793 2.2:+2139126 None:intergenic
CTCAGAATCTGCCGCTCCAA+TGG 0.465779 2.2:+2139288 None:intergenic
ACGGAGAAGAGCCTGACGTC+CGG 0.467710 2.2:+2138913 None:intergenic
CCACCTGCTACTAGTATCAT+TGG 0.480418 2.2:-2139106 MS.gene36336:CDS
GATAAGCAAGAGATTTGGTT+GGG 0.481364 2.2:-2139257 MS.gene36336:CDS
AGAGCCTGACGTCCGGGGAA+TGG 0.482518 2.2:+2138920 None:intergenic
AAATACAAGGGGAAGATGTT+GGG 0.485271 2.2:-2139490 None:intergenic
CGGAGAAGAGCCTGACGTCC+GGG 0.490784 2.2:+2138914 None:intergenic
GACCTTCTCGGCACTTCTAA+GGG 0.491235 2.2:-2139196 MS.gene36336:CDS
CTGACGTCCGGGGAATGGAT+TGG 0.498352 2.2:+2138925 None:intergenic
AGCGGCAGATTCTGAGCTGC+AGG 0.508668 2.2:-2139281 MS.gene36336:CDS
CCACATTGTTGTCCTTCTGT+TGG 0.509399 2.2:+2138993 None:intergenic
TGCCATGGTTGATGGCCTTA+CGG 0.514572 2.2:+2138894 None:intergenic
TCGCTGAAATTGACAAGTCT+CGG 0.520757 2.2:+2139163 None:intergenic
TTCCGTCGAGCTACCTCAAG+TGG 0.521226 2.2:-2139462 MS.gene36336:CDS
AGCACGCGAGTTTGAGGAGT+GGG 0.522721 2.2:-2139422 MS.gene36336:CDS
ACAGTAGTAGTAGGACTAAG+AGG 0.528829 2.2:-2139069 MS.gene36336:CDS
GCCATTGCCAATCCATTCCC+CGG 0.538546 2.2:-2138932 MS.gene36336:CDS
ACGGTAGTAACAGTAGTAGT+AGG 0.539912 2.2:-2139078 MS.gene36336:CDS
CTCCCTTAGAAGTGCCGAGA+AGG 0.555291 2.2:+2139194 None:intergenic
TGCTCCAACCAACTTTGTCA+AGG 0.565623 2.2:+2139230 None:intergenic
ACGTCAGGCTCTTCTCCGTA+AGG 0.566162 2.2:-2138909 MS.gene36336:CDS
AAATGAAGCGCACCAACAGA+AGG 0.567882 2.2:-2139005 MS.gene36336:CDS
AGATAAGCAAGAGATTTGGT+TGG 0.569741 2.2:-2139258 MS.gene36336:CDS
TAGTAGTAGGACTAAGAGGA+TGG 0.572795 2.2:-2139065 MS.gene36336:CDS
CTACCAATGATACTAGTAGC+AGG 0.580274 2.2:+2139103 None:intergenic
TCCGGGGAATGGATTGGCAA+TGG 0.584712 2.2:+2138931 None:intergenic
TGGGATAACCTTGACAAAGT+TGG 0.586673 2.2:-2139238 MS.gene36336:CDS
ATAACCTTGACAAAGTTGGT+TGG 0.591237 2.2:-2139234 MS.gene36336:CDS
TTCTCGGCACTTCTAAGGGA+GGG 0.601983 2.2:-2139192 MS.gene36336:CDS
TTTAGCTTAGGATGCTGCCA+TGG 0.602732 2.2:+2138879 None:intergenic
CTCCGTAAGGCCATCAACCA+TGG 0.607049 2.2:-2138896 MS.gene36336:CDS
TCTACAAAGACCGCATTGAG+TGG 0.608441 2.2:-2139366 MS.gene36336:CDS
ACTAGTATCATTGGTAGCAA+CGG 0.610173 2.2:-2139097 MS.gene36336:CDS
TCCGTCGAGCTACCTCAAGT+GGG 0.610493 2.2:-2139461 MS.gene36336:CDS
CCAATGATACTAGTAGCAGG+TGG 0.627861 2.2:+2139106 None:intergenic
CGAGACAGAAAATGAGATCG+AGG 0.635307 2.2:-2139033 MS.gene36336:CDS
TGATACTAGTAGCAGGTGGG+TGG 0.637051 2.2:+2139110 None:intergenic
CTACAAAGACCGCATTGAGT+GGG 0.639133 2.2:-2139365 MS.gene36336:CDS
GGAGAAGAGCCTGACGTCCG+GGG 0.642345 2.2:+2138915 None:intergenic
CTTCTCGGCACTTCTAAGGG+AGG 0.649267 2.2:-2139193 MS.gene36336:CDS
ACTTGAGGTAGCTCGACGGA+AGG 0.664751 2.2:+2139464 None:intergenic
CAATGATACTAGTAGCAGGT+GGG 0.664856 2.2:+2139107 None:intergenic
TGCTGCTAGATCCATTGGAG+CGG 0.676565 2.2:-2139299 MS.gene36336:CDS
ACCCACTTGAGGTAGCTCGA+CGG 0.680643 2.2:+2139460 None:intergenic
CCAACAGAAGGACAACAATG+TGG 0.706083 2.2:-2138993 MS.gene36336:CDS
GAGCAAACTCCCACTCAATG+CGG 0.708509 2.2:+2139356 None:intergenic
GAGCACGCGAGTTTGAGGAG+TGG 0.717265 2.2:-2139423 MS.gene36336:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTGCTCTTAATGTGTTAAA+TGG - chr2.2:2139015-2139034 MS.gene36336:CDS 25.0%
! AAAGTTGATTTCGACCTTCT+CGG - chr2.2:2139147-2139166 MS.gene36336:CDS 35.0%
! ACTAGTATCATTGGTAGCAA+CGG - chr2.2:2139258-2139277 MS.gene36336:CDS 35.0%
! AGATAAGCAAGAGATTTGGT+TGG - chr2.2:2139097-2139116 MS.gene36336:CDS 35.0%
! ATAACCTTGACAAAGTTGGT+TGG - chr2.2:2139121-2139140 MS.gene36336:CDS 35.0%
! GATAAGCAAGAGATTTGGTT+GGG - chr2.2:2139098-2139117 MS.gene36336:CDS 35.0%
!!! AGTTTTTTGAAACCCACTTG+AGG + chr2.2:2138909-2138928 None:intergenic 35.0%
ACAGTAGTAGTAGGACTAAG+AGG - chr2.2:2139286-2139305 MS.gene36336:CDS 40.0%
ACGGTAGTAACAGTAGTAGT+AGG - chr2.2:2139277-2139296 MS.gene36336:CDS 40.0%
CAATGATACTAGTAGCAGGT+GGG + chr2.2:2139251-2139270 None:intergenic 40.0%
CAATGCGGTCTTTGTAGATT+GGG + chr2.2:2138987-2139006 None:intergenic 40.0%
CAGGAGATAAGCAAGAGATT+TGG - chr2.2:2139093-2139112 MS.gene36336:CDS 40.0%
CTACCAATGATACTAGTAGC+AGG + chr2.2:2139255-2139274 None:intergenic 40.0%
CTTTGTAGATTGGGTCTAGA+AGG + chr2.2:2138978-2138997 None:intergenic 40.0%
TAGTAGTAGGACTAAGAGGA+TGG - chr2.2:2139290-2139309 MS.gene36336:CDS 40.0%
TCAATGCGGTCTTTGTAGAT+TGG + chr2.2:2138988-2139007 None:intergenic 40.0%
TCGCTGAAATTGACAAGTCT+CGG + chr2.2:2139195-2139214 None:intergenic 40.0%
TGGGATAACCTTGACAAAGT+TGG - chr2.2:2139117-2139136 MS.gene36336:CDS 40.0%
AAATGAAGCGCACCAACAGA+AGG - chr2.2:2139350-2139369 MS.gene36336:CDS 45.0%
CCAACAGAAGGACAACAATG+TGG - chr2.2:2139362-2139381 MS.gene36336:CDS 45.0%
CCAATGATACTAGTAGCAGG+TGG + chr2.2:2139252-2139271 None:intergenic 45.0%
CCACATTGTTGTCCTTCTGT+TGG + chr2.2:2139365-2139384 None:intergenic 45.0%
CCACCTGCTACTAGTATCAT+TGG - chr2.2:2139249-2139268 MS.gene36336:CDS 45.0%
CGAGACAGAAAATGAGATCG+AGG - chr2.2:2139322-2139341 MS.gene36336:CDS 45.0%
CTACAAAGACCGCATTGAGT+GGG - chr2.2:2138990-2139009 MS.gene36336:CDS 45.0%
TCTACAAAGACCGCATTGAG+TGG - chr2.2:2138989-2139008 MS.gene36336:CDS 45.0%
TGCTCCAACCAACTTTGTCA+AGG + chr2.2:2139128-2139147 None:intergenic 45.0%
!! GTTAGGTTGGGTGTTGGTAT+TGG + chr2.2:2139226-2139245 None:intergenic 45.0%
GACCTTCTCGGCACTTCTAA+GGG - chr2.2:2139159-2139178 MS.gene36336:CDS 50.0%
GAGCAAACTCCCACTCAATG+CGG + chr2.2:2139002-2139021 None:intergenic 50.0%
TAGGATGCTGCCATGGTTGA+TGG + chr2.2:2139472-2139491 None:intergenic 50.0%
TCTGCTGCTGCTAGATCCAT+TGG - chr2.2:2139051-2139070 MS.gene36336:CDS 50.0%
TGATACTAGTAGCAGGTGGG+TGG + chr2.2:2139248-2139267 None:intergenic 50.0%
TGCTGCTAGATCCATTGGAG+CGG - chr2.2:2139056-2139075 MS.gene36336:CDS 50.0%
! CTTAAGAGCACGCGAGTTTG+AGG - chr2.2:2138927-2138946 MS.gene36336:CDS 50.0%
! TTCTCGGCACTTCTAAGGGA+GGG - chr2.2:2139163-2139182 MS.gene36336:CDS 50.0%
!! TGCCATGGTTGATGGCCTTA+CGG + chr2.2:2139464-2139483 None:intergenic 50.0%
ACCCACTTGAGGTAGCTCGA+CGG + chr2.2:2138898-2138917 None:intergenic 55.0%
ACGTCAGGCTCTTCTCCGTA+AGG - chr2.2:2139446-2139465 MS.gene36336:CDS 55.0%
ACTTGAGGTAGCTCGACGGA+AGG + chr2.2:2138894-2138913 None:intergenic 55.0%
AGCACGCGAGTTTGAGGAGT+GGG - chr2.2:2138933-2138952 MS.gene36336:CDS 55.0%
AGCAGGTGGGTGGTTAGGTT+GGG + chr2.2:2139238-2139257 None:intergenic 55.0%
CGACCTTCTCGGCACTTCTA+AGG - chr2.2:2139158-2139177 MS.gene36336:CDS 55.0%
CTAGTAGCAGGTGGGTGGTT+AGG + chr2.2:2139243-2139262 None:intergenic 55.0%
CTCAGAATCTGCCGCTCCAA+TGG + chr2.2:2139070-2139089 None:intergenic 55.0%
CTCCGTAAGGCCATCAACCA+TGG - chr2.2:2139459-2139478 MS.gene36336:CDS 55.0%
GCCATTGCCAATCCATTCCC+CGG - chr2.2:2139423-2139442 MS.gene36336:CDS 55.0%
TAGCAGGTGGGTGGTTAGGT+TGG + chr2.2:2139239-2139258 None:intergenic 55.0%
TCCGTCGAGCTACCTCAAGT+GGG - chr2.2:2138894-2138913 MS.gene36336:CDS 55.0%
TTCCGTCGAGCTACCTCAAG+TGG - chr2.2:2138893-2138912 MS.gene36336:CDS 55.0%
! CTTCTCGGCACTTCTAAGGG+AGG - chr2.2:2139162-2139181 MS.gene36336:CDS 55.0%
!! CTCCCTTAGAAGTGCCGAGA+AGG + chr2.2:2139164-2139183 None:intergenic 55.0%
!! TCCGGGGAATGGATTGGCAA+TGG + chr2.2:2139427-2139446 None:intergenic 55.0%
!! TGGGTGGTTAGGTTGGGTGT+TGG + chr2.2:2139232-2139251 None:intergenic 55.0%
ACGGAGAAGAGCCTGACGTC+CGG + chr2.2:2139445-2139464 None:intergenic 60.0%
AGCGGCAGATTCTGAGCTGC+AGG - chr2.2:2139074-2139093 MS.gene36336:CDS 60.0%
CAATCCATTCCCCGGACGTC+AGG - chr2.2:2139431-2139450 MS.gene36336:CDS 60.0%
CTGACGTCCGGGGAATGGAT+TGG + chr2.2:2139433-2139452 None:intergenic 60.0%
GAGCACGCGAGTTTGAGGAG+TGG - chr2.2:2138932-2138951 MS.gene36336:CDS 60.0%
AGAGCCTGACGTCCGGGGAA+TGG + chr2.2:2139438-2139457 None:intergenic 65.0%
CGGAGAAGAGCCTGACGTCC+GGG + chr2.2:2139444-2139463 None:intergenic 65.0%
GGAGAAGAGCCTGACGTCCG+GGG + chr2.2:2139443-2139462 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 2138880 2139497 2138880 ID=MS.gene36336
chr2.2 mRNA 2138880 2139497 2138880 ID=MS.gene36336.t1;Parent=MS.gene36336
chr2.2 exon 2138880 2139497 2138880 ID=MS.gene36336.t1.exon1;Parent=MS.gene36336.t1
chr2.2 CDS 2138880 2139497 2138880 ID=cds.MS.gene36336.t1;Parent=MS.gene36336.t1
Gene Sequence

>MS.gene36336

ATGTTGGGAAGCCTTCCGTCGAGCTACCTCAAGTGGGTTTCAAAAAACTTAAGAGCACGCGAGTTTGAGGAGTGGGCAATTCTTGCAGATCAAGTCCTTCTAGACCCAATCTACAAAGACCGCATTGAGTGGGAGTTTGCTCTTAATGTGTTAAATGGCAACAACATTTCTTCTGCTGCTGCTAGATCCATTGGAGCGGCAGATTCTGAGCTGCAGGAGATAAGCAAGAGATTTGGTTGGGATAACCTTGACAAAGTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAAGGGAGGGAGAATTCCGAGACTTGTCAATTTCAGCGACAGTGACGCCAATACCAACACCCAACCTAACCACCCACCTGCTACTAGTATCATTGGTAGCAACGGTAGTAACAGTAGTAGTAGGACTAAGAGGATGGAGAGAAGAGCGAGACAGAAAATGAGATCGAGGACGACAAATGAAGCGCACCAACAGAAGGACAACAATGTGGTTGATATTGAAGAAGTTGAAGATGATAACATTAATATAGCCATTGCCAATCCATTCCCCGGACGTCAGGCTCTTCTCCGTAAGGCCATCAACCATGGCAGCATCCTAAGCTAA

Protein sequence

>MS.gene36336.t1

MLGSLPSSYLKWVSKNLRAREFEEWAILADQVLLDPIYKDRIEWEFALNVLNGNNISSAAARSIGAADSELQEISKRFGWDNLDKVGWSKVDFDLLGTSKGGRIPRLVNFSDSDANTNTQPNHPPATSIIGSNGSNSSSRTKRMERRARQKMRSRTTNEAHQQKDNNVVDIEEVEDDNINIAIANPFPGRQALLRKAINHGSILS