AlfalfaGEDB | MS.gene75549

M. sativa cultivar XinJiangDaYe / MS.gene75549

KEGG: K02723
GO: GO:0003674 GO:0005488 GO:0005575 GO:0008150 GO:0008152 GO:0009521 GO:0009523 GO:0009987 GO:0015979 GO:0016021 GO:0030145 GO:0031224 GO:0032991 GO:0043167 GO:0043169 GO:0044237 GO:0046872 GO:0046914 GO:0098796 GO:0110165
PFAM: PF06298.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75549.t1	XP_003601864.2	95.5	199	6	2	1	198	1	197	1.50E-89	339

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75549.t1	P80470	51.0	208	81	7	1	197	1	198	6.3e-36	152.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75549.t1	G7J2K8	95.5	199	6	2	1	198	1	197	1.1e-89	339.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene75549	0.805175	1.52E-49	-1.69E-46
MS.gene049171	MS.gene75549	0.882032	1.46E-70	-1.69E-46
MS.gene049173	MS.gene75549	0.858637	7.18E-63	-1.69E-46
MS.gene049217	MS.gene75549	0.875455	3.04E-68	-1.69E-46
MS.gene049222	MS.gene75549	0.90374	2.54E-79	-1.69E-46
MS.gene049232	MS.gene75549	0.831171	1.94E-55	-1.69E-46
MS.gene049285	MS.gene75549	0.92489	3.84E-90	-1.69E-46
MS.gene049320	MS.gene75549	0.931456	3.68E-94	-1.69E-46
MS.gene049369	MS.gene75549	0.801172	1.02E-48	-1.69E-46
MS.gene04939	MS.gene75549	0.838021	3.68E-57	-1.69E-46
MS.gene049427	MS.gene75549	0.843757	1.15E-58	-1.69E-46
MS.gene049609	MS.gene75549	0.899063	2.87E-77	-1.69E-46
MS.gene049614	MS.gene75549	0.832569	8.77E-56	-1.69E-46
MS.gene049627	MS.gene75549	0.820718	5.92E-53	-1.69E-46
MS.gene049633	MS.gene75549	0.881607	2.09E-70	-1.69E-46
MS.gene049805	MS.gene75549	0.867065	1.80E-65	-1.69E-46
MS.gene05010	MS.gene75549	0.855975	4.40E-62	-1.69E-46
MS.gene050163	MS.gene75549	0.909915	3.36E-82	-1.69E-46
MS.gene050165	MS.gene75549	0.906206	1.90E-80	-1.69E-46
MS.gene050170	MS.gene75549	0.821626	3.65E-53	-1.69E-46
MS.gene050216	MS.gene75549	0.804697	1.91E-49	-1.69E-46
MS.gene050323	MS.gene75549	0.836178	1.09E-56	-1.69E-46
MS.gene050333	MS.gene75549	0.890485	9.44E-74	-1.69E-46
MS.gene050432	MS.gene75549	0.801273	9.73E-49	-1.69E-46
MS.gene050444	MS.gene75549	0.838793	2.33E-57	-1.69E-46
MS.gene050496	MS.gene75549	0.831021	2.11E-55	-1.69E-46
MS.gene050504	MS.gene75549	0.901794	1.87E-78	-1.69E-46
MS.gene050518	MS.gene75549	0.893141	8.28E-75	-1.69E-46
MS.gene050538	MS.gene75549	0.867031	1.85E-65	-1.69E-46
MS.gene050577	MS.gene75549	0.859732	3.37E-63	-1.69E-46
MS.gene050593	MS.gene75549	0.833148	6.30E-56	-1.69E-46
MS.gene050612	MS.gene75549	0.918181	2.14E-86	-1.69E-46
MS.gene050662	MS.gene75549	0.808973	2.38E-50	-1.69E-46
MS.gene050675	MS.gene75549	0.880962	3.56E-70	-1.69E-46
MS.gene050699	MS.gene75549	0.900364	7.89E-78	-1.69E-46
MS.gene050747	MS.gene75549	0.881524	2.23E-70	-1.69E-46
MS.gene050767	MS.gene75549	0.825863	3.72E-54	-1.69E-46
MS.gene050770	MS.gene75549	0.83037	3.05E-55	-1.69E-46
MS.gene050772	MS.gene75549	0.927877	6.36E-92	-1.69E-46
MS.gene05080	MS.gene75549	0.870748	1.15E-66	-1.69E-46
MS.gene05081	MS.gene75549	0.815809	7.64E-52	-1.69E-46
MS.gene050868	MS.gene75549	0.859086	5.28E-63	-1.69E-46
MS.gene050871	MS.gene75549	0.856906	2.34E-62	-1.69E-46
MS.gene050876	MS.gene75549	0.836929	7.01E-57	-1.69E-46
MS.gene050904	MS.gene75549	0.815039	1.13E-51	-1.69E-46
MS.gene050950	MS.gene75549	0.891476	3.84E-74	-1.69E-46
MS.gene050986	MS.gene75549	0.893189	7.92E-75	-1.69E-46
MS.gene05100	MS.gene75549	0.888295	6.70E-73	-1.69E-46
MS.gene05105	MS.gene75549	0.840628	7.76E-58	-1.69E-46
MS.gene051127	MS.gene75549	-0.805259	1.46E-49	-1.69E-46
MS.gene051228	MS.gene75549	0.86854	6.05E-66	-1.69E-46
MS.gene051268	MS.gene75549	0.80854	2.94E-50	-1.69E-46
MS.gene051317	MS.gene75549	0.845534	3.83E-59	-1.69E-46
MS.gene051434	MS.gene75549	0.814741	1.32E-51	-1.69E-46
MS.gene051551	MS.gene75549	0.867192	1.64E-65	-1.69E-46
MS.gene051617	MS.gene75549	0.854213	1.43E-61	-1.69E-46
MS.gene051651	MS.gene75549	-0.803549	3.31E-49	-1.69E-46
MS.gene051666	MS.gene75549	0.841813	3.78E-58	-1.69E-46
MS.gene051694	MS.gene75549	0.824589	7.44E-54	-1.69E-46
MS.gene051810	MS.gene75549	0.952459	2.31E-110	-1.69E-46
MS.gene051838	MS.gene75549	0.829039	6.43E-55	-1.69E-46
MS.gene051858	MS.gene75549	0.88133	2.63E-70	-1.69E-46
MS.gene051874	MS.gene75549	0.803148	4.00E-49	-1.69E-46
MS.gene051949	MS.gene75549	0.857341	1.74E-62	-1.69E-46
MS.gene052164	MS.gene75549	0.856577	2.93E-62	-1.69E-46
MS.gene052271	MS.gene75549	0.887652	1.18E-72	-1.69E-46
MS.gene052290	MS.gene75549	0.8194	1.19E-52	-1.69E-46
MS.gene052379	MS.gene75549	0.885859	5.65E-72	-1.69E-46
MS.gene052421	MS.gene75549	0.82803	1.13E-54	-1.69E-46
MS.gene052482	MS.gene75549	0.833106	6.45E-56	-1.69E-46
MS.gene052484	MS.gene75549	0.824531	7.67E-54	-1.69E-46
MS.gene052486	MS.gene75549	0.843087	1.74E-58	-1.69E-46
MS.gene052503	MS.gene75549	0.827952	1.18E-54	-1.69E-46
MS.gene052615	MS.gene75549	0.91588	3.48E-85	-1.69E-46
MS.gene052670	MS.gene75549	0.82208	2.87E-53	-1.69E-46
MS.gene052822	MS.gene75549	0.819428	1.17E-52	-1.69E-46
MS.gene052823	MS.gene75549	0.823668	1.22E-53	-1.69E-46
MS.gene052825	MS.gene75549	0.81498	1.17E-51	-1.69E-46
MS.gene052870	MS.gene75549	0.871477	6.63E-67	-1.69E-46
MS.gene052872	MS.gene75549	0.88099	3.48E-70	-1.69E-46
MS.gene052916	MS.gene75549	0.873242	1.71E-67	-1.69E-46
MS.gene052966	MS.gene75549	0.837056	6.51E-57	-1.69E-46
MS.gene053009	MS.gene75549	0.857435	1.64E-62	-1.69E-46
MS.gene053103	MS.gene75549	0.806206	9.21E-50	-1.69E-46
MS.gene053104	MS.gene75549	0.84693	1.59E-59	-1.69E-46
MS.gene053117	MS.gene75549	0.800164	1.64E-48	-1.69E-46
MS.gene053203	MS.gene75549	0.844196	8.78E-59	-1.69E-46
MS.gene053207	MS.gene75549	0.874423	6.83E-68	-1.69E-46
MS.gene053213	MS.gene75549	0.924811	4.28E-90	-1.69E-46
MS.gene053286	MS.gene75549	0.920705	9.12E-88	-1.69E-46
MS.gene053322	MS.gene75549	0.86583	4.44E-65	-1.69E-46
MS.gene053420	MS.gene75549	-0.820577	6.37E-53	-1.69E-46
MS.gene053436	MS.gene75549	0.835366	1.75E-56	-1.69E-46
MS.gene053479	MS.gene75549	0.832516	9.04E-56	-1.69E-46
MS.gene053480	MS.gene75549	0.840663	7.59E-58	-1.69E-46
MS.gene053488	MS.gene75549	-0.813361	2.66E-51	-1.69E-46
MS.gene053561	MS.gene75549	-0.835008	2.15E-56	-1.69E-46
MS.gene053574	MS.gene75549	0.904629	1.01E-79	-1.69E-46
MS.gene053579	MS.gene75549	0.800998	1.11E-48	-1.69E-46
MS.gene053626	MS.gene75549	0.816555	5.21E-52	-1.69E-46
MS.gene053632	MS.gene75549	0.88368	3.65E-71	-1.69E-46
MS.gene053824	MS.gene75549	0.871747	5.40E-67	-1.69E-46
MS.gene053916	MS.gene75549	0.857227	1.88E-62	-1.69E-46
MS.gene054374	MS.gene75549	0.838039	3.64E-57	-1.69E-46
MS.gene054408	MS.gene75549	0.89927	2.34E-77	-1.69E-46
MS.gene054595	MS.gene75549	0.830944	2.21E-55	-1.69E-46
MS.gene054600	MS.gene75549	0.901385	2.83E-78	-1.69E-46
MS.gene054616	MS.gene75549	-0.802614	5.16E-49	-1.69E-46
MS.gene054642	MS.gene75549	0.93016	2.45E-93	-1.69E-46
MS.gene05470	MS.gene75549	0.863253	2.84E-64	-1.69E-46
MS.gene054927	MS.gene75549	0.825383	4.83E-54	-1.69E-46
MS.gene05494	MS.gene75549	0.8384	2.94E-57	-1.69E-46
MS.gene054954	MS.gene75549	0.903518	3.19E-79	-1.69E-46
MS.gene05505	MS.gene75549	0.875093	4.04E-68	-1.69E-46
MS.gene055154	MS.gene75549	-0.845486	3.94E-59	-1.69E-46
MS.gene055206	MS.gene75549	0.866676	2.39E-65	-1.69E-46
MS.gene055212	MS.gene75549	0.870358	1.55E-66	-1.69E-46
MS.gene055224	MS.gene75549	0.848647	5.36E-60	-1.69E-46
MS.gene055289	MS.gene75549	0.855249	7.16E-62	-1.69E-46
MS.gene055362	MS.gene75549	0.917289	6.36E-86	-1.69E-46
MS.gene055397	MS.gene75549	0.854292	1.36E-61	-1.69E-46
MS.gene055412	MS.gene75549	0.810328	1.22E-50	-1.69E-46
MS.gene055499	MS.gene75549	0.870602	1.29E-66	-1.69E-46
MS.gene055502	MS.gene75549	0.909908	3.38E-82	-1.69E-46
MS.gene055577	MS.gene75549	0.876584	1.24E-68	-1.69E-46
MS.gene055636	MS.gene75549	0.821794	3.34E-53	-1.69E-46
MS.gene055638	MS.gene75549	0.836114	1.13E-56	-1.69E-46
MS.gene055647	MS.gene75549	0.829854	4.08E-55	-1.69E-46
MS.gene055722	MS.gene75549	0.899988	1.15E-77	-1.69E-46
MS.gene055748	MS.gene75549	0.867156	1.68E-65	-1.69E-46
MS.gene055750	MS.gene75549	0.861044	1.35E-63	-1.69E-46
MS.gene055751	MS.gene75549	0.868535	6.07E-66	-1.69E-46
MS.gene055776	MS.gene75549	0.850093	2.12E-60	-1.69E-46
MS.gene05589	MS.gene75549	0.860803	1.60E-63	-1.69E-46
MS.gene05592	MS.gene75549	0.866911	2.02E-65	-1.69E-46
MS.gene055941	MS.gene75549	0.859564	3.79E-63	-1.69E-46
MS.gene055956	MS.gene75549	-0.824962	6.07E-54	-1.69E-46
MS.gene056083	MS.gene75549	0.85789	1.20E-62	-1.69E-46
MS.gene056374	MS.gene75549	0.883362	4.78E-71	-1.69E-46
MS.gene056409	MS.gene75549	0.811312	7.46E-51	-1.69E-46
MS.gene056446	MS.gene75549	0.835792	1.37E-56	-1.69E-46
MS.gene056448	MS.gene75549	0.831582	1.54E-55	-1.69E-46
MS.gene056449	MS.gene75549	0.842088	3.20E-58	-1.69E-46
MS.gene056487	MS.gene75549	0.822261	2.60E-53	-1.69E-46
MS.gene056504	MS.gene75549	0.877078	8.38E-69	-1.69E-46
MS.gene056510	MS.gene75549	0.880967	3.55E-70	-1.69E-46
MS.gene056549	MS.gene75549	0.826889	2.12E-54	-1.69E-46
MS.gene056609	MS.gene75549	0.805614	1.23E-49	-1.69E-46
MS.gene056610	MS.gene75549	0.81001	1.43E-50	-1.69E-46
MS.gene056657	MS.gene75549	0.931786	2.25E-94	-1.69E-46
MS.gene056692	MS.gene75549	0.870008	2.02E-66	-1.69E-46
MS.gene056897	MS.gene75549	0.858594	7.40E-63	-1.69E-46
MS.gene056898	MS.gene75549	0.81342	2.58E-51	-1.69E-46
MS.gene056903	MS.gene75549	0.815639	8.34E-52	-1.69E-46
MS.gene056946	MS.gene75549	0.830437	2.94E-55	-1.69E-46
MS.gene056947	MS.gene75549	0.823548	1.31E-53	-1.69E-46
MS.gene057011	MS.gene75549	0.824467	7.95E-54	-1.69E-46
MS.gene057048	MS.gene75549	0.805594	1.24E-49	-1.69E-46
MS.gene057076	MS.gene75549	0.886403	3.53E-72	-1.69E-46
MS.gene057129	MS.gene75549	0.884569	1.72E-71	-1.69E-46
MS.gene057146	MS.gene75549	0.81929	1.26E-52	-1.69E-46
MS.gene057246	MS.gene75549	0.824108	9.65E-54	-1.69E-46
MS.gene057331	MS.gene75549	0.8771	8.24E-69	-1.69E-46
MS.gene057416	MS.gene75549	0.875304	3.42E-68	-1.69E-46
MS.gene057658	MS.gene75549	0.83315	6.29E-56	-1.69E-46
MS.gene057831	MS.gene75549	0.864869	8.91E-65	-1.69E-46
MS.gene05787	MS.gene75549	0.818601	1.80E-52	-1.69E-46
MS.gene057926	MS.gene75549	0.815907	7.27E-52	-1.69E-46
MS.gene058019	MS.gene75549	0.810275	1.25E-50	-1.69E-46
MS.gene058130	MS.gene75549	0.909387	6.03E-82	-1.69E-46
MS.gene058323	MS.gene75549	0.855613	5.61E-62	-1.69E-46
MS.gene058338	MS.gene75549	0.844281	8.33E-59	-1.69E-46
MS.gene05837	MS.gene75549	0.880828	3.98E-70	-1.69E-46
MS.gene058419	MS.gene75549	0.92703	2.07E-91	-1.69E-46
MS.gene058544	MS.gene75549	0.889801	1.75E-73	-1.69E-46
MS.gene058723	MS.gene75549	0.921172	5.03E-88	-1.69E-46
MS.gene058773	MS.gene75549	0.910784	1.27E-82	-1.69E-46
MS.gene058838	MS.gene75549	0.855622	5.58E-62	-1.69E-46
MS.gene05883	MS.gene75549	0.841166	5.60E-58	-1.69E-46
MS.gene058840	MS.gene75549	-0.805415	1.35E-49	-1.69E-46
MS.gene059022	MS.gene75549	0.922293	1.19E-88	-1.69E-46
MS.gene059023	MS.gene75549	0.892545	1.44E-74	-1.69E-46
MS.gene059029	MS.gene75549	0.90049	6.95E-78	-1.69E-46
MS.gene059079	MS.gene75549	0.824874	6.37E-54	-1.69E-46
MS.gene05912	MS.gene75549	0.800397	1.47E-48	-1.69E-46
MS.gene05913	MS.gene75549	0.873089	1.93E-67	-1.69E-46
MS.gene05914	MS.gene75549	0.82429	8.74E-54	-1.69E-46
MS.gene059305	MS.gene75549	0.824381	8.33E-54	-1.69E-46
MS.gene05934	MS.gene75549	0.849281	3.58E-60	-1.69E-46
MS.gene059367	MS.gene75549	0.810285	1.24E-50	-1.69E-46
MS.gene059536	MS.gene75549	0.848264	6.85E-60	-1.69E-46
MS.gene059667	MS.gene75549	0.829721	4.39E-55	-1.69E-46
MS.gene059677	MS.gene75549	0.928213	3.96E-92	-1.69E-46
MS.gene059718	MS.gene75549	0.915133	8.45E-85	-1.69E-46
MS.gene059756	MS.gene75549	0.849798	2.57E-60	-1.69E-46
MS.gene059801	MS.gene75549	0.813141	2.97E-51	-1.69E-46
MS.gene059858	MS.gene75549	0.824685	7.06E-54	-1.69E-46
MS.gene059865	MS.gene75549	0.892614	1.35E-74	-1.69E-46
MS.gene059902	MS.gene75549	0.879728	9.83E-70	-1.69E-46
MS.gene059960	MS.gene75549	0.855521	5.97E-62	-1.69E-46
MS.gene06000	MS.gene75549	0.805827	1.11E-49	-1.69E-46
MS.gene060341	MS.gene75549	0.825949	3.55E-54	-1.69E-46
MS.gene060482	MS.gene75549	0.823687	1.21E-53	-1.69E-46
MS.gene060703	MS.gene75549	0.88512	1.07E-71	-1.69E-46
MS.gene060728	MS.gene75549	0.835732	1.41E-56	-1.69E-46
MS.gene060762	MS.gene75549	0.896227	4.52E-76	-1.69E-46
MS.gene060778	MS.gene75549	0.834921	2.27E-56	-1.69E-46
MS.gene06077	MS.gene75549	0.888554	5.32E-73	-1.69E-46
MS.gene060804	MS.gene75549	0.860154	2.52E-63	-1.69E-46
MS.gene060818	MS.gene75549	0.839082	1.96E-57	-1.69E-46
MS.gene060835	MS.gene75549	0.839127	1.91E-57	-1.69E-46
MS.gene060837	MS.gene75549	0.805622	1.22E-49	-1.69E-46
MS.gene06092	MS.gene75549	0.879884	8.66E-70	-1.69E-46
MS.gene060982	MS.gene75549	0.869169	3.79E-66	-1.69E-46
MS.gene061041	MS.gene75549	0.85272	3.84E-61	-1.69E-46
MS.gene061134	MS.gene75549	0.836943	6.96E-57	-1.69E-46
MS.gene061150	MS.gene75549	0.887887	9.61E-73	-1.69E-46
MS.gene061171	MS.gene75549	0.808152	3.56E-50	-1.69E-46
MS.gene061200	MS.gene75549	0.861901	7.41E-64	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75549.t1	MTR_3g086230	98.492	199	0	2	1	198	1	197	2.83e-136	379
MS.gene75549.t1	MTR_8g089610	64.563	206	58	9	1	196	1	201	2.04e-62	192
MS.gene75549.t1	MTR_2g046810	50.968	155	43	10	8	153	1	131	7.47e-24	92.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75549.t1	AT1G67740	51.562	192	73	5	12	197	10	187	3.24e-44	145

Find 50 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGGACTTGGACTTGGACT+TGG	0.271973	3.1:-69086391	MS.gene75549:CDS
CTTGGTGGACTTGCAGCTTC+AGG	0.300451	3.1:-69086373	MS.gene75549:CDS
GTGATTGTTGGGCTTGGACT+TGG	0.338557	3.1:-69086403	MS.gene75549:CDS
TGACACAAAGTTGGTGTTCT+TGG	0.338685	3.1:+69086633	None:intergenic
TCACCTTCACTTGCAATTGC+AGG	0.361555	3.1:-69086613	MS.gene75549:CDS
CTGATCTCATCCTGTTGATC+TGG	0.394420	3.1:+69086209	None:intergenic
CGTTACGCATACGGTTGATT+TGG	0.394692	3.1:+69086434	None:intergenic
TTTGTTGTTGCACCAGCTAT+AGG	0.399173	3.1:-69086493	MS.gene75549:CDS
GTTGGGCTTGGACTTGGACT+TGG	0.401202	3.1:-69086397	MS.gene75549:CDS
GAAGCATCTGCTAGTGAGAT+TGG	0.403127	3.1:-69086340	MS.gene75549:CDS
ACTTGGGTAGGCCTGTGTTT+AGG	0.404849	3.1:+69086740	None:intergenic
ACGCAAAAGGTGTGATTGTT+GGG	0.409047	3.1:-69086414	MS.gene75549:CDS
CTGCTCTGAACCAGATCAAC+AGG	0.409433	3.1:-69086219	MS.gene75549:CDS
ATGCTGCTGGAAGTGACAAC+AGG	0.409635	3.1:-69086306	MS.gene75549:CDS
AACCGTATGCGTAACGCAAA+AGG	0.427923	3.1:-69086427	MS.gene75549:CDS
GGACTTGGACTTGGACTTGG+TGG	0.429062	3.1:-69086388	MS.gene75549:CDS
TTGTTGCACCAGCTATCCTT+TGG	0.431369	3.1:-69086264	MS.gene75549:CDS
TGTTGCACCAGCTATCCTTT+GGG	0.438183	3.1:-69086263	MS.gene75549:CDS
AACGCAAAAGGTGTGATTGT+TGG	0.441975	3.1:-69086415	MS.gene75549:CDS
GGTTTGATTGCTGATGCTGC+TGG	0.461729	3.1:-69086319	MS.gene75549:CDS
TTGTTGCACCAGCTATAGGT+TGG	0.462026	3.1:-69086489	MS.gene75549:CDS
TGTTGCACCAGCTATAGGTT+GGG	0.466375	3.1:-69086488	MS.gene75549:CDS
AAAGGTGTGATTGTTGGGCT+TGG	0.466916	3.1:-69086409	MS.gene75549:CDS
CGGTTGATTTGGTTCAAAGC+TGG	0.475315	3.1:+69086445	None:intergenic
CTGTTGATCTGGTTCAGAGC+AGG	0.484163	3.1:+69086220	None:intergenic
TTGGTTGAGACAAGACCCTT+TGG	0.496099	3.1:+69086652	None:intergenic
TACAGCACCCAAAGGATAGC+TGG	0.516105	3.1:+69086256	None:intergenic
TGGTAGGTTCTGCATGGTGG+TGG	0.518556	3.1:+69086672	None:intergenic
AAGCCGCGTCGGAGGTTGCA+AGG	0.534226	3.1:+69086575	None:intergenic
TGGACTTGCAGCTTCAGGTA+TGG	0.535553	3.1:-69086368	MS.gene75549:CDS
AGCTGCGAAAGCCGCGTCGG+AGG	0.541223	3.1:+69086567	None:intergenic
ACCCTTTGGTAGGTTCTGCA+TGG	0.542276	3.1:+69086666	None:intergenic
AACAAAACCCAACCTATAGC+TGG	0.551151	3.1:+69086481	None:intergenic
AGCCGCGTCGGAGGTTGCAA+GGG	0.564916	3.1:+69086576	None:intergenic
TTCATCAATGGCGGCAACAA+TGG	0.573337	3.1:-69086785	None:intergenic
ACCATGCAGAACCTACCAAA+GGG	0.580172	3.1:-69086667	MS.gene75549:CDS
TGCTGCTGGAAGTGACAACA+GGG	0.593134	3.1:-69086305	MS.gene75549:CDS
TTGAGACAAGACCCTTTGGT+AGG	0.600634	3.1:+69086656	None:intergenic
CAAATAGCAGAAATAGCTGA+AGG	0.603764	3.1:-69086541	MS.gene75549:CDS
TGTTGATGTTAGCTTAGTAG+TGG	0.612701	3.1:+69086702	None:intergenic
CTTTGGTAGGTTCTGCATGG+TGG	0.614099	3.1:+69086669	None:intergenic
TTGAGCTGCGAAAGCCGCGT+CGG	0.615667	3.1:+69086564	None:intergenic
CACCATGCAGAACCTACCAA+AGG	0.623574	3.1:-69086668	MS.gene75549:CDS
AACACAAAGTGCCTAAACAC+AGG	0.637457	3.1:-69086751	MS.gene75549:CDS
ATCCCTTGCAACCTCCGACG+CGG	0.643167	3.1:-69086578	MS.gene75549:CDS
AAATATTGTACAGCACCCAA+AGG	0.643861	3.1:+69086248	None:intergenic
AAGTGAAGGTGACACAAAGT+TGG	0.674791	3.1:+69086624	None:intergenic
GCTCCTGCAATTGCAAGTGA+AGG	0.683304	3.1:+69086610	None:intergenic
ATAGCTGAAGGTGATAACAG+AGG	0.718762	3.1:-69086529	MS.gene75549:CDS
GCTGCTGGAAGTGACAACAG+GGG	0.839610	3.1:-69086304	MS.gene75549:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATATTGTACAGCACCCAA+AGG	+	chr3.1:69086737-69086756	None:intergenic	35.0%
	CAAATAGCAGAAATAGCTGA+AGG	-	chr3.1:69086441-69086460	MS.gene75549:CDS	35.0%
!	GTGCTAAGATTTTGCAACTT+GGG	+	chr3.1:69086261-69086280	None:intergenic	35.0%
!	TAAGATTTTGCAACTTGGGT+AGG	+	chr3.1:69086257-69086276	None:intergenic	35.0%
!	TGTTGATGTTAGCTTAGTAG+TGG	+	chr3.1:69086283-69086302	None:intergenic	35.0%
	AACAAAACCCAACCTATAGC+TGG	+	chr3.1:69086504-69086523	None:intergenic	40.0%
	AAGTGAAGGTGACACAAAGT+TGG	+	chr3.1:69086361-69086380	None:intergenic	40.0%
	ATAGCTGAAGGTGATAACAG+AGG	-	chr3.1:69086453-69086472	MS.gene75549:CDS	40.0%
!	AACACAAAGTGCCTAAACAC+AGG	-	chr3.1:69086231-69086250	MS.gene75549:CDS	40.0%
!	AACGCAAAAGGTGTGATTGT+TGG	-	chr3.1:69086567-69086586	MS.gene75549:CDS	40.0%
!	ACGCAAAAGGTGTGATTGTT+GGG	-	chr3.1:69086568-69086587	MS.gene75549:CDS	40.0%
!	GGTGCTAAGATTTTGCAACT+TGG	+	chr3.1:69086262-69086281	None:intergenic	40.0%
!!	TGACACAAAGTTGGTGTTCT+TGG	+	chr3.1:69086352-69086371	None:intergenic	40.0%
!!	TTTGTTGTTGCACCAGCTAT+AGG	-	chr3.1:69086489-69086508	MS.gene75549:CDS	40.0%
	AACCGTATGCGTAACGCAAA+AGG	-	chr3.1:69086555-69086574	MS.gene75549:CDS	45.0%
	ACCATGCAGAACCTACCAAA+GGG	-	chr3.1:69086315-69086334	MS.gene75549:CDS	45.0%
	CGTTACGCATACGGTTGATT+TGG	+	chr3.1:69086551-69086570	None:intergenic	45.0%
	CTGATCTCATCCTGTTGATC+TGG	+	chr3.1:69086776-69086795	None:intergenic	45.0%
	TCACCTTCACTTGCAATTGC+AGG	-	chr3.1:69086369-69086388	MS.gene75549:CDS	45.0%
	TGTTGCACCAGCTATAGGTT+GGG	-	chr3.1:69086494-69086513	MS.gene75549:CDS	45.0%
	TGTTGCACCAGCTATCCTTT+GGG	-	chr3.1:69086719-69086738	MS.gene75549:CDS	45.0%
	TTGAGACAAGACCCTTTGGT+AGG	+	chr3.1:69086329-69086348	None:intergenic	45.0%
	TTGGTTGAGACAAGACCCTT+TGG	+	chr3.1:69086333-69086352	None:intergenic	45.0%
	TTGTTGCACCAGCTATAGGT+TGG	-	chr3.1:69086493-69086512	MS.gene75549:CDS	45.0%
	TTGTTGCACCAGCTATCCTT+TGG	-	chr3.1:69086718-69086737	MS.gene75549:CDS	45.0%
!	AAAGGTGTGATTGTTGGGCT+TGG	-	chr3.1:69086573-69086592	MS.gene75549:CDS	45.0%
!	CACCTTTTGCGTTACGCATA+CGG	+	chr3.1:69086560-69086579	None:intergenic	45.0%
!	GAAGCATCTGCTAGTGAGAT+TGG	-	chr3.1:69086642-69086661	MS.gene75549:CDS	45.0%
!!	CGGTTGATTTGGTTCAAAGC+TGG	+	chr3.1:69086540-69086559	None:intergenic	45.0%
	ACCCTTTGGTAGGTTCTGCA+TGG	+	chr3.1:69086319-69086338	None:intergenic	50.0%
	ACTTGGGTAGGCCTGTGTTT+AGG	+	chr3.1:69086245-69086264	None:intergenic	50.0%
	ATGCTGCTGGAAGTGACAAC+AGG	-	chr3.1:69086676-69086695	MS.gene75549:CDS	50.0%
	CACCATGCAGAACCTACCAA+AGG	-	chr3.1:69086314-69086333	MS.gene75549:CDS	50.0%
	CTTGGACTTGGACTTGGACT+TGG	-	chr3.1:69086591-69086610	MS.gene75549:CDS	50.0%
	CTTTGGTAGGTTCTGCATGG+TGG	+	chr3.1:69086316-69086335	None:intergenic	50.0%
	GCTCCTGCAATTGCAAGTGA+AGG	+	chr3.1:69086375-69086394	None:intergenic	50.0%
	GGTTTGATTGCTGATGCTGC+TGG	-	chr3.1:69086663-69086682	MS.gene75549:CDS	50.0%
	GTGATTGTTGGGCTTGGACT+TGG	-	chr3.1:69086579-69086598	MS.gene75549:CDS	50.0%
	TACAGCACCCAAAGGATAGC+TGG	+	chr3.1:69086729-69086748	None:intergenic	50.0%
	TGCTGCTGGAAGTGACAACA+GGG	-	chr3.1:69086677-69086696	MS.gene75549:CDS	50.0%
	TGGACTTGCAGCTTCAGGTA+TGG	-	chr3.1:69086614-69086633	MS.gene75549:CDS	50.0%
!	CTGCTCTGAACCAGATCAAC+AGG	-	chr3.1:69086763-69086782	MS.gene75549:CDS	50.0%
!	CTGTTGATCTGGTTCAGAGC+AGG	+	chr3.1:69086765-69086784	None:intergenic	50.0%
	GCTGCTGGAAGTGACAACAG+GGG	-	chr3.1:69086678-69086697	MS.gene75549:CDS	55.0%
	GGACTTGGACTTGGACTTGG+TGG	-	chr3.1:69086594-69086613	MS.gene75549:CDS	55.0%
	GTTGGGCTTGGACTTGGACT+TGG	-	chr3.1:69086585-69086604	MS.gene75549:CDS	55.0%
	TGGTAGGTTCTGCATGGTGG+TGG	+	chr3.1:69086313-69086332	None:intergenic	55.0%
!!	CTTGGTGGACTTGCAGCTTC+AGG	-	chr3.1:69086609-69086628	MS.gene75549:CDS	55.0%
	ATCCCTTGCAACCTCCGACG+CGG	-	chr3.1:69086404-69086423	MS.gene75549:CDS	60.0%
	TTGAGCTGCGAAAGCCGCGT+CGG	+	chr3.1:69086421-69086440	None:intergenic	60.0%
	AAGCCGCGTCGGAGGTTGCA+AGG	+	chr3.1:69086410-69086429	None:intergenic	65.0%
	AGCCGCGTCGGAGGTTGCAA+GGG	+	chr3.1:69086409-69086428	None:intergenic	65.0%
	AGCTGCGAAAGCCGCGTCGG+AGG	+	chr3.1:69086418-69086437	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	69086204	69086800	69086204	ID=MS.gene75549
chr3.1	mRNA	69086204	69086800	69086204	ID=MS.gene75549.t1;Parent=MS.gene75549
chr3.1	exon	69086204	69086800	69086204	ID=MS.gene75549.t1.exon1;Parent=MS.gene75549.t1
chr3.1	CDS	69086204	69086800	69086204	ID=cds.MS.gene75549.t1;Parent=MS.gene75549.t1

Gene Sequence

>MS.gene75549

ATGGCGGCAACAATGGCAATGATGATCAACACAAAGTGCCTAAACACAGGCCTACCCAAGTTGCAAAATCTTAGCACCACTACTAAGCTAACATCAACATGCAAAACCACCACCATGCAGAACCTACCAAAGGGTCTTGTCTCAACCAAGAACACCAACTTTGTGTCACCTTCACTTGCAATTGCAGGAGCAATATTTTCATCCCTTGCAACCTCCGACGCGGCTTTCGCAGCTCAACAAATAGCAGAAATAGCTGAAGGTGATAACAGAGGAACACTTCTGCTGTTTGTTGTTGCACCAGCTATAGGTTGGGTTTTGTTCAACATATTGCAGCCAGCTTTGAACCAAATCAACCGTATGCGTAACGCAAAAGGTGTGATTGTTGGGCTTGGACTTGGACTTGGACTTGGTGGACTTGCAGCTTCAGGTATGGTGTCAGAAGCATCTGCTAGTGAGATTGGTTTGATTGCTGATGCTGCTGGAAGTGACAACAGGGGAACACTTCTGCTGTTTGTTGTTGCACCAGCTATCCTTTGGGTGCTGTACAATATTTTGCAGCCTGCTCTGAACCAGATCAACAGGATGAGATCAGATTAA

Protein sequence

>MS.gene75549.t1

MAATMAMMINTKCLNTGLPKLQNLSTTTKLTSTCKTTTMQNLPKGLVSTKNTNFVSPSLAIAGAIFSSLATSDAAFAAQQIAEIAEGDNRGTLLLFVVAPAIGWVLFNILQPALNQINRMRNAKGVIVGLGLGLGLGGLAASGMVSEASASEIGLIADAAGSDNRGTLLLFVVAPAILWVLYNILQPALNQINRMRSD