AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46798


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46798.t1 MTR_3g028150 92.958 213 13 1 31 243 32 242 6.94e-138 387
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46798.t1 AT3G63540 78.261 138 30 0 105 242 3 140 3.78e-82 242

Find 46 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATATTTAACTTTCCTTTC+TGG 0.121727 3.3:-20693531 MS.gene46798:CDS
TCTTTCTCTGTTCTCTTCTT+TGG 0.152693 3.3:+20693552 None:intergenic
GGCGGCGGAGGAGGAGAAAA+TGG 0.222541 3.3:+20693914 None:intergenic
AAACGTTCCTTCCAACCTTC+TGG 0.272048 3.3:+20693627 None:intergenic
AATATTTAACTTTCCTTTCT+GGG 0.289515 3.3:-20693530 MS.gene46798:CDS
TTAATATCAAAAGTTGAAAA+AGG 0.291067 3.3:-20693606 MS.gene46798:CDS
GGTGGTGGTGGCGGCGGAGG+AGG 0.295244 3.3:+20693905 None:intergenic
TTCTGCTGAGTCTTTCTTGT+TGG 0.307382 3.3:+20693665 None:intergenic
GGTGGTGGTGGTGGCGGCGG+AGG 0.354249 3.3:+20693902 None:intergenic
TGTGGAGGTTGTTGTGGTGG+TGG 0.388270 3.3:+20693887 None:intergenic
GGTTGTTGTGGTGGTGGTGG+TGG 0.401000 3.3:+20693893 None:intergenic
GGAGGTTGTTGTGGTGGTGG+TGG 0.402045 3.3:+20693890 None:intergenic
GTTAAGGTGGTGGTTATTAG+AGG 0.416358 3.3:+20693819 None:intergenic
GGTGGTTGTGGAGGTTGTTG+TGG 0.419764 3.3:+20693881 None:intergenic
TTGTTCAAAACAGCATCTTT+TGG 0.430262 3.3:+20693492 None:intergenic
TATTATGTTTATGAGATTGA+TGG 0.453881 3.3:-20693378 MS.gene46798:CDS
GGTTGTGGAGGTTGTTGTGG+TGG 0.476092 3.3:+20693884 None:intergenic
AGGAGATGATTCTGCTGCTA+TGG 0.480110 3.3:+20693749 None:intergenic
GAAGAAGTGAAAGATGATAA+AGG 0.482410 3.3:-20693408 MS.gene46798:CDS
TCTTTATTCCACTCAGGAGT+AGG 0.485770 3.3:+20693285 None:intergenic
GTACCGTAGTAGAGATTGTA+AGG 0.491822 3.3:+20693729 None:intergenic
GCGGCGGAGGAGGAGAAAAT+GGG 0.496977 3.3:+20693915 None:intergenic
GCAGCAAACTACGGCGGCTA+CGG 0.501712 3.3:-20693696 MS.gene46798:CDS
AGGTGGTGATGTTGTGAGAA+TGG 0.508602 3.3:+20693773 None:intergenic
AATTTGCAAGATATTATTAG+TGG 0.515902 3.3:-20693450 MS.gene46798:CDS
TCCTTATCTTTATTCCACTC+AGG 0.519893 3.3:+20693279 None:intergenic
TGATATGGTGGTGGTTGTGG+AGG 0.520434 3.3:+20693872 None:intergenic
TTGTGTTTGAATGAGATAAG+AGG 0.520933 3.3:+20693846 None:intergenic
TGCTGCTGCTGTTAAGGTGG+TGG 0.525546 3.3:+20693809 None:intergenic
TCCTGAGTGGAATAAAGATA+AGG 0.526998 3.3:-20693280 MS.gene46798:CDS
TGCTGCTGCTGCTGTTAAGG+TGG 0.548707 3.3:+20693806 None:intergenic
TCTACGACGTTCCAGAAGGT+TGG 0.565732 3.3:-20693638 MS.gene46798:CDS
GCGGAGGAGGAGAAAATGGG+TGG 0.566411 3.3:+20693918 None:intergenic
GCAAACTACGGCGGCTACGG+CGG 0.573351 3.3:-20693693 MS.gene46798:CDS
GAGGAGGAGAAAATGGGTGG+TGG 0.579804 3.3:+20693921 None:intergenic
CGACGTTCCAGAAGGTTGGA+AGG 0.585452 3.3:-20693634 MS.gene46798:CDS
GCAGCAAGTGCAGCAAACTA+CGG 0.594999 3.3:-20693705 MS.gene46798:CDS
TCTCCTTACAATCTCTACTA+CGG 0.600009 3.3:-20693732 MS.gene46798:CDS
GATGATTCTGCTGCTATGGA+AGG 0.605999 3.3:+20693753 None:intergenic
GCAAGTGCAGCAAACTACGG+CGG 0.619443 3.3:-20693702 MS.gene46798:CDS
TACATCTACGACGTTCCAGA+AGG 0.628307 3.3:-20693642 MS.gene46798:CDS
CTAACTGACGAAACCCAGAA+AGG 0.634618 3.3:+20693517 None:intergenic
TTAATGCTCCTACTCCTGAG+TGG 0.644053 3.3:-20693293 MS.gene46798:CDS
GATTCTGCTGCTATGGAAGG+TGG 0.667537 3.3:+20693756 None:intergenic
TGGTGATGTTGTGAGAATGG+TGG 0.683595 3.3:+20693776 None:intergenic
TGGTGATGATGAGAAAATTG+TGG 0.687506 3.3:+20693941 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATCAAAAGTTGAAAA+AGG - chr3.3:20693575-20693594 MS.gene46798:CDS 15.0%
!! AATTTGCAAGATATTATTAG+TGG - chr3.3:20693731-20693750 MS.gene46798:CDS 20.0%
!! TATTATGTTTATGAGATTGA+TGG - chr3.3:20693803-20693822 MS.gene46798:CDS 20.0%
!!! AATATTTAACTTTCCTTTCT+GGG - chr3.3:20693651-20693670 MS.gene46798:CDS 20.0%
!! AAAGTTGAAAAAGGTACAAA+TGG - chr3.3:20693584-20693603 MS.gene46798:CDS 25.0%
!! GAATATTTAACTTTCCTTTC+TGG - chr3.3:20693650-20693669 MS.gene46798:CDS 25.0%
GAAGAAGTGAAAGATGATAA+AGG - chr3.3:20693773-20693792 MS.gene46798:CDS 30.0%
TTGTGTTTGAATGAGATAAG+AGG + chr3.3:20693338-20693357 None:intergenic 30.0%
! TTGTTCAAAACAGCATCTTT+TGG + chr3.3:20693692-20693711 None:intergenic 30.0%
!! TGAGATAAGAGGTTTTGATA+TGG + chr3.3:20693327-20693346 None:intergenic 30.0%
TCCTGAGTGGAATAAAGATA+AGG - chr3.3:20693901-20693920 MS.gene46798:CDS 35.0%
TCCTTATCTTTATTCCACTC+AGG + chr3.3:20693905-20693924 None:intergenic 35.0%
TCTCCTTACAATCTCTACTA+CGG - chr3.3:20693449-20693468 MS.gene46798:CDS 35.0%
TCTTTCTCTGTTCTCTTCTT+TGG + chr3.3:20693632-20693651 None:intergenic 35.0%
! TGGTGATGATGAGAAAATTG+TGG + chr3.3:20693243-20693262 None:intergenic 35.0%
!! GATAAGAGGTTTTGATATGG+TGG + chr3.3:20693324-20693343 None:intergenic 35.0%
GTACCGTAGTAGAGATTGTA+AGG + chr3.3:20693455-20693474 None:intergenic 40.0%
TCTTTATTCCACTCAGGAGT+AGG + chr3.3:20693899-20693918 None:intergenic 40.0%
TTCTGCTGAGTCTTTCTTGT+TGG + chr3.3:20693519-20693538 None:intergenic 40.0%
! GTTAAGGTGGTGGTTATTAG+AGG + chr3.3:20693365-20693384 None:intergenic 40.0%
!! AAGAGGTTTTGATATGGTGG+TGG + chr3.3:20693321-20693340 None:intergenic 40.0%
!!! TTTTGATATGGTGGTGGTTG+TGG + chr3.3:20693315-20693334 None:intergenic 40.0%
AAACGTTCCTTCCAACCTTC+TGG + chr3.3:20693557-20693576 None:intergenic 45.0%
AGGAGATGATTCTGCTGCTA+TGG + chr3.3:20693435-20693454 None:intergenic 45.0%
CTAACTGACGAAACCCAGAA+AGG + chr3.3:20693667-20693686 None:intergenic 45.0%
GATGATTCTGCTGCTATGGA+AGG + chr3.3:20693431-20693450 None:intergenic 45.0%
TACATCTACGACGTTCCAGA+AGG - chr3.3:20693539-20693558 MS.gene46798:CDS 45.0%
TTAATGCTCCTACTCCTGAG+TGG - chr3.3:20693888-20693907 MS.gene46798:CDS 45.0%
! AGGTGGTGATGTTGTGAGAA+TGG + chr3.3:20693411-20693430 None:intergenic 45.0%
! TGGTGATGTTGTGAGAATGG+TGG + chr3.3:20693408-20693427 None:intergenic 45.0%
GATTCTGCTGCTATGGAAGG+TGG + chr3.3:20693428-20693447 None:intergenic 50.0%
GCAGCAAGTGCAGCAAACTA+CGG - chr3.3:20693476-20693495 MS.gene46798:CDS 50.0%
TCTACGACGTTCCAGAAGGT+TGG - chr3.3:20693543-20693562 MS.gene46798:CDS 50.0%
!! TGATATGGTGGTGGTTGTGG+AGG + chr3.3:20693312-20693331 None:intergenic 50.0%
AGCTGCTGCTGCTGCTGTTA+AGG + chr3.3:20693381-20693400 None:intergenic 55.0%
CGACGTTCCAGAAGGTTGGA+AGG - chr3.3:20693547-20693566 MS.gene46798:CDS 55.0%
GAGGAGGAGAAAATGGGTGG+TGG + chr3.3:20693263-20693282 None:intergenic 55.0%
GCAAGTGCAGCAAACTACGG+CGG - chr3.3:20693479-20693498 MS.gene46798:CDS 55.0%
GGTGGTTGTGGAGGTTGTTG+TGG + chr3.3:20693303-20693322 None:intergenic 55.0%
GGTTGTGGAGGTTGTTGTGG+TGG + chr3.3:20693300-20693319 None:intergenic 55.0%
TGCTGCTGCTGCTGTTAAGG+TGG + chr3.3:20693378-20693397 None:intergenic 55.0%
TGCTGCTGCTGTTAAGGTGG+TGG + chr3.3:20693375-20693394 None:intergenic 55.0%
TGTGGAGGTTGTTGTGGTGG+TGG + chr3.3:20693297-20693316 None:intergenic 55.0%
GCAGCAAACTACGGCGGCTA+CGG - chr3.3:20693485-20693504 MS.gene46798:CDS 60.0%
GCGGAGGAGGAGAAAATGGG+TGG + chr3.3:20693266-20693285 None:intergenic 60.0%
GCGGCGGAGGAGGAGAAAAT+GGG + chr3.3:20693269-20693288 None:intergenic 60.0%
!! GGAGGTTGTTGTGGTGGTGG+TGG + chr3.3:20693294-20693313 None:intergenic 60.0%
!! GGTTGTTGTGGTGGTGGTGG+TGG + chr3.3:20693291-20693310 None:intergenic 60.0%
!! TGTTGTGGTGGTGGTGGTGG+CGG + chr3.3:20693288-20693307 None:intergenic 60.0%
GCAAACTACGGCGGCTACGG+CGG - chr3.3:20693488-20693507 MS.gene46798:CDS 65.0%
GGCGGCGGAGGAGGAGAAAA+TGG + chr3.3:20693270-20693289 None:intergenic 65.0%
!! TGTGGTGGTGGTGGTGGCGG+CGG + chr3.3:20693285-20693304 None:intergenic 70.0%
!!! GGTGGTGGTGGCGGCGGAGG+AGG + chr3.3:20693279-20693298 None:intergenic 80.0%
!!! GGTGGTGGTGGTGGCGGCGG+AGG + chr3.3:20693282-20693301 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 20693236 20693967 20693236 ID=MS.gene46798
chr3.3 mRNA 20693236 20693967 20693236 ID=MS.gene46798.t1;Parent=MS.gene46798
chr3.3 exon 20693236 20693967 20693236 ID=MS.gene46798.t1.exon1;Parent=MS.gene46798.t1
chr3.3 CDS 20693236 20693967 20693236 ID=cds.MS.gene46798.t1;Parent=MS.gene46798.t1
Gene Sequence

>MS.gene46798

ATGGCCACAATTTTCTCATCATCACCACCACCCATTTTCTCCTCCTCCGCCGCCACCACCACCACCACAACAACCTCCACAACCACCACCATATCAAAACCTCTTATCTCATTCAAACACAAACAACCTCTAATAACCACCACCTTAACAGCAGCAGCAGCAGCTATAGCCACCATTCTCACAACATCACCACCTTCCATAGCAGCAGAATCATCTCCTTACAATCTCTACTACGGTACAGCAGCAAGTGCAGCAAACTACGGCGGCTACGGCGGAAACTCCAACAAGAAAGACTCAGCAGAATACATCTACGACGTTCCAGAAGGTTGGAAGGAACGTTTAATATCAAAAGTTGAAAAAGGTACAAATGGAACAGACAGTGAGTTCTACAATCCAAAGAAGAGAACAGAGAAAGAATATTTAACTTTCCTTTCTGGGTTTCGTCAGTTAGCACCAAAAGATGCTGTTTTGAACAACTTAGCACTTTCTGATGTGAATTTGCAAGATATTATTAGTGGTGCTGATAATGTGAGTTCTGAAGAAGTGAAAGATGATAAAGGTCAAGTTTATTATGTTTATGAGATTGATGGTGTTGCTTATCATAGCTTGATTTCTGTTACTTGTGCTAATAATAAGCTTTATGCTCATTTTGTTAATGCTCCTACTCCTGAGTGGAATAAAGATAAGGATCTTCTCACTCATGTTCATAAGTCTTTTAAGACTATTGTATAA

Protein sequence

>MS.gene46798.t1

MATIFSSSPPPIFSSSAATTTTTTTSTTTTISKPLISFKHKQPLITTTLTAAAAAIATILTTSPPSIAAESSPYNLYYGTAASAANYGGYGGNSNKKDSAEYIYDVPEGWKERLISKVEKGTNGTDSEFYNPKKRTEKEYLTFLSGFRQLAPKDAVLNNLALSDVNLQDIISGADNVSSEEVKDDKGQVYYVYEIDGVAYHSLISVTCANNKLYAHFVNAPTPEWNKDKDLLTHVHKSFKTIV