AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0353880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0353880 MtrunA17_Chr3g0089161 86.667 105 12 1 1 105 1 103 1.43e-49 155
Msa0353880 MtrunA17_Chr3g0089161 82.796 93 15 1 16 107 95 187 1.58e-45 145
Msa0353880 MtrunA17_Chr4g0019291 71.296 108 28 3 1 106 1 107 8.38e-44 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0353880 AT1G51400.1 60.000 110 37 4 1 107 1 106 1.71e-31 107
Msa0353880 AT3G21055.1 54.630 108 41 4 1 106 1 102 3.60e-24 89.0
Msa0353880 AT3G21055.2 54.630 108 41 4 1 106 41 142 9.13e-24 89.0

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCTGTTTGCTCTGTTGC+TGG 0.185357 3_1:-24582889 Msa0353880:CDS
CTGCTGTTTGCTCTGTTGCT+GGG 0.300217 3_1:-24582888 Msa0353880:CDS
ATCCTCAATCCTTGGCTTCC+CGG 0.339335 3_1:-24583064 Msa0353880:CDS
CTCTGTTGCTGGGGTTGCAA+TGG 0.391387 3_1:-24582878 Msa0353880:CDS
GCTTCCCGGCTGTAACCAAC+CGG 0.484721 3_1:-24583050 Msa0353880:CDS
GTTGGCATAGTGACACAAAC+AGG 0.491066 3_1:+24582805 None:intergenic
AATGCTGTCCGAGCAGTTGA+AGG 0.499596 3_1:-24582988 Msa0353880:CDS
TGCTGTTTGCTCTGTTGCTG+GGG 0.508878 3_1:-24582887 Msa0353880:CDS
ACCATGACATCCTCAATCCT+TGG 0.515033 3_1:-24583072 Msa0353880:CDS
AACAACAAAGAGGGCAGCAA+TGG 0.517711 3_1:-24582943 Msa0353880:CDS
TCTTCTCCTCTGCGATGCTA+CGG 0.528081 3_1:+24583023 None:intergenic
AGCCGGGAAGCCAAGGATTG+AGG 0.543329 3_1:+24583062 None:intergenic
TCTGCGATGCTACGGATGAC+CGG 0.544879 3_1:+24583031 None:intergenic
ACAAAGAGGGCAGCAATGGA+AGG 0.546411 3_1:-24582939 Msa0353880:CDS
TGGTTACAGCCGGGAAGCCA+AGG 0.559386 3_1:+24583055 None:intergenic
GATGACCGGTTGGTTACAGC+CGG 0.561463 3_1:+24583045 None:intergenic
ATGCTGTCCGAGCAGTTGAA+GGG 0.565277 3_1:-24582987 Msa0353880:CDS
GCCAAGGATTGAGGATGTCA+TGG 0.568229 3_1:+24583071 None:intergenic
GTGTCACTATGCCAACAGCT+AGG 0.576409 3_1:-24582798 Msa0353880:CDS
TTGCGACAAATCCTAGCTGT+TGG 0.581063 3_1:+24582787 None:intergenic
TGCTGTCCGAGCAGTTGAAG+GGG 0.590682 3_1:-24582986 Msa0353880:CDS
CGATGCTACGGATGACCGGT+TGG 0.597909 3_1:+24583035 None:intergenic
CAAATATGATAACAACAAAG+AGG 0.606053 3_1:-24582953 Msa0353880:CDS
AAATATGATAACAACAAAGA+GGG 0.608783 3_1:-24582952 Msa0353880:CDS
ATGACCGGTTGGTTACAGCC+GGG 0.647755 3_1:+24583046 None:intergenic
GGTCATCCGTAGCATCGCAG+AGG 0.728401 3_1:-24583029 Msa0353880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGATAACAACAAAGA+GGG - chr3_1:24582915-24582934 Msa0353880:CDS 20.0%
! CAAATATGATAACAACAAAG+AGG - chr3_1:24582914-24582933 Msa0353880:CDS 25.0%
!!! ATACTTTTTCTTTGCTTCTG+GGG + chr3_1:24583039-24583058 None:intergenic 30.0%
!!! CATACTTTTTCTTTGCTTCT+GGG + chr3_1:24583040-24583059 None:intergenic 30.0%
!!! GCATACTTTTTCTTTGCTTC+TGG + chr3_1:24583041-24583060 None:intergenic 35.0%
GCAGATGATGAACCAAAAAG+AGG - chr3_1:24583011-24583030 Msa0353880:CDS 40.0%
ACCATGACATCCTCAATCCT+TGG - chr3_1:24582795-24582814 Msa0353880:CDS 45.0%
! TCTTTTCCCCTTCAACTGCT+CGG + chr3_1:24582890-24582909 None:intergenic 45.0%
AACAACAAAGAGGGCAGCAA+TGG - chr3_1:24582924-24582943 Msa0353880:CDS 45.0%
GTTGGCATAGTGACACAAAC+AGG + chr3_1:24583065-24583084 None:intergenic 45.0%
TTGCGACAAATCCTAGCTGT+TGG + chr3_1:24583083-24583102 None:intergenic 45.0%
GCCAAGGATTGAGGATGTCA+TGG + chr3_1:24582799-24582818 None:intergenic 50.0%
ATCCTCAATCCTTGGCTTCC+CGG - chr3_1:24582803-24582822 Msa0353880:CDS 50.0%
TCTTCTCCTCTGCGATGCTA+CGG + chr3_1:24582847-24582866 None:intergenic 50.0%
AATGCTGTCCGAGCAGTTGA+AGG - chr3_1:24582879-24582898 Msa0353880:CDS 50.0%
ATGCTGTCCGAGCAGTTGAA+GGG - chr3_1:24582880-24582899 Msa0353880:CDS 50.0%
ACAAAGAGGGCAGCAATGGA+AGG - chr3_1:24582928-24582947 Msa0353880:CDS 50.0%
! CTGCTGTTTGCTCTGTTGCT+GGG - chr3_1:24582979-24582998 Msa0353880:CDS 50.0%
! TGCTGTTTGCTCTGTTGCTG+GGG - chr3_1:24582980-24582999 Msa0353880:CDS 50.0%
!!! GCTTCTGGGGTACCTCTTTT+TGG + chr3_1:24583026-24583045 None:intergenic 50.0%
! GTGTCACTATGCCAACAGCT+AGG - chr3_1:24583069-24583088 Msa0353880:CDS 50.0%
! ATGACCGGTTGGTTACAGCC+GGG + chr3_1:24582824-24582843 None:intergenic 55.0%
! GATGACCGGTTGGTTACAGC+CGG + chr3_1:24582825-24582844 None:intergenic 55.0%
TCTGCGATGCTACGGATGAC+CGG + chr3_1:24582839-24582858 None:intergenic 55.0%
TGCTGTCCGAGCAGTTGAAG+GGG - chr3_1:24582881-24582900 Msa0353880:CDS 55.0%
! GCTGCTGTTTGCTCTGTTGC+TGG - chr3_1:24582978-24582997 Msa0353880:CDS 55.0%
CTCTGTTGCTGGGGTTGCAA+TGG - chr3_1:24582989-24583008 Msa0353880:CDS 55.0%
AGCCGGGAAGCCAAGGATTG+AGG + chr3_1:24582808-24582827 None:intergenic 60.0%
TGGTTACAGCCGGGAAGCCA+AGG + chr3_1:24582815-24582834 None:intergenic 60.0%
GCTTCCCGGCTGTAACCAAC+CGG - chr3_1:24582817-24582836 Msa0353880:CDS 60.0%
CGATGCTACGGATGACCGGT+TGG + chr3_1:24582835-24582854 None:intergenic 60.0%
GGTCATCCGTAGCATCGCAG+AGG - chr3_1:24582838-24582857 Msa0353880:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 24582783 24583106 24582783 ID=Msa0353880;Name=Msa0353880
chr3_1 mRNA 24582783 24583106 24582783 ID=Msa0353880-mRNA-1;Parent=Msa0353880;Name=Msa0353880-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|107
chr3_1 exon 24582783 24583106 24582783 ID=Msa0353880-mRNA-1:exon:5461;Parent=Msa0353880-mRNA-1
chr3_1 CDS 24582783 24583106 24582783 ID=Msa0353880-mRNA-1:cds;Parent=Msa0353880-mRNA-1
Gene Sequence

>Msa0353880

ATGGCATCAATCACCATGACATCCTCAATCCTTGGCTTCCCGGCTGTAACCAACCGGTCATCCGTAGCATCGCAGAGGAGAAGACTTGTCGTCGTCAATGCTGTCCGAGCAGTTGAAGGGGAAAAGATAATCAAATATGATAACAACAAAGAGGGCAGCAATGGAAGGAGAGACTTGATGTTTGCTGCAGCAGCAGCTGCTGTTTGCTCTGTTGCTGGGGTTGCAATGGCAGATGATGAACCAAAAAGAGGTACCCCAGAAGCAAAGAAAAAGTATGCTCCTGTTTGTGTCACTATGCCAACAGCTAGGATTTGTCGCAATTGA

Protein sequence

>Msa0353880

MASITMTSSILGFPAVTNRSSVASQRRRLVVVNAVRAVEGEKIIKYDNNKEGSNGRRDLMFAAAAAAVCSVAGVAMADDEPKRGTPEAKKKYAPVCVTMPTARICRN*