AlfalfaGEDB | Msa0181010

M. sativa cultivar zhongmu-4 / Msa0181010

KEGG: K16908
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0015979 GO:0019684 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
PFAM: PF01113.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181010	sp\|Q9FJ82\|DAPB3_ARATH	67.434	304	62	5	1	273	1	298	2.41e-136	389

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181010	G7IAQ3	89.735	302	2	1	1	273	1	302	0.0	536

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0181010	0.868670	5.495017e-66	-8.615850e-47
Msa0000780	Msa0181010	0.825698	4.068189e-54	-8.615850e-47
Msa0001420	Msa0181010	0.836434	9.382311e-57	-8.615850e-47
Msa0001460	Msa0181010	0.857095	2.061019e-62	-8.615850e-47
Msa0001510	Msa0181010	0.844065	9.521164e-59	-8.615850e-47
Msa0001540	Msa0181010	0.902099	1.370253e-78	-8.615850e-47
Msa0002060	Msa0181010	0.878617	2.429203e-69	-8.615850e-47
Msa0003470	Msa0181010	0.832882	7.334501e-56	-8.615850e-47
Msa0005160	Msa0181010	0.803787	2.950946e-49	-8.615850e-47
Msa0006050	Msa0181010	0.812896	3.365995e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181010	MtrunA17_Chr1g0203761	89.735	302	2	1	1	273	1	302	0.0	536

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181010	AT5G52100.1	67.434	304	62	5	1	273	1	298	2.45e-137	389

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Find 103 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCAAGGGGTCAAGTTT+TGG	0.246017	1_4:+69092605	Msa0181010:intron
AAAGTGAACTGTTGTACTAG+AGG	0.590884	1_4:-69092670	None:intergenic
AAATGACCTTACCATGGTTT+TGG	0.224480	1_4:+69091244	Msa0181010:CDS
AAGTGAACTGTTGTACTAGA+GGG	0.580769	1_4:-69092669	None:intergenic
AATGACTTTATTGTATGTTC+TGG	0.411404	1_4:-69093350	None:intergenic
AATGGAGCAGCAAAGGAAAT+AGG	0.336907	1_4:+69090857	Msa0181010:CDS
ACACACATATAGCCGAAGCT+GGG	0.628344	1_4:-69090557	None:intergenic
ACATTGTCATATACCGCGGA+AGG	0.549371	1_4:-69091421	None:intergenic
ACCATCGTATAATTTGGGTA+GGG	0.620846	1_4:-69093484	None:intergenic
ACCCTACCCAAATTATACGA+TGG	0.676435	1_4:+69093483	Msa0181010:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAGAATTCAAGTAGTTGA+TGG	-	chr1_4:69090661-69090680	None:intergenic	30.0%
CAACTACTTGAATTCTCAAA+TGG	+	chr1_4:69090662-69090681	Msa0181010:CDS	30.0%
GCCTTTATATTGCTTTGTAT+AGG	-	chr1_4:69090732-69090751	None:intergenic	30.0%
CTATTGATACTCAACATGTT+GGG	+	chr1_4:69090933-69090952	Msa0181010:CDS	30.0%
TATTGATACTCAACATGTTG+GGG	+	chr1_4:69090934-69090953	Msa0181010:CDS	30.0%
AATGAGCTGAAAACAACTTA+TGG	-	chr1_4:69091016-69091035	None:intergenic	30.0%
ATGGTAAGGTCATTTAGTAT+AGG	-	chr1_4:69091239-69091258	None:intergenic	30.0%
TATACTAAATGACCTTACCA+TGG	+	chr1_4:69091238-69091257	Msa0181010:CDS	30.0%
TGAATTAGCAATAACAAACG+TGG	+	chr1_4:69091295-69091314	Msa0181010:intron	30.0%
GGTATATGACAATGTAAAAC+AGG	+	chr1_4:69091429-69091448	Msa0181010:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	69090547	69093826	69090547	ID=Msa0181010;Name=Msa0181010
chr1_4	mRNA	69090547	69093826	69090547	ID=Msa0181010-mRNA-1;Parent=Msa0181010;Name=Msa0181010-mRNA-1;_AED=0.12;_eAED=0.12;_QI=83\|0.87\|1\|1\|0.75\|0.88\|9\|647\|273
chr1_4	exon	69090547	69090749	69090547	ID=Msa0181010-mRNA-1:exon:22935;Parent=Msa0181010-mRNA-1
chr1_4	exon	69090848	69090970	69090848	ID=Msa0181010-mRNA-1:exon:22936;Parent=Msa0181010-mRNA-1
chr1_4	exon	69091206	69091280	69091206	ID=Msa0181010-mRNA-1:exon:22937;Parent=Msa0181010-mRNA-1
chr1_4	exon	69091385	69091450	69091385	ID=Msa0181010-mRNA-1:exon:22938;Parent=Msa0181010-mRNA-1
chr1_4	exon	69091788	69091883	69091788	ID=Msa0181010-mRNA-1:exon:22939;Parent=Msa0181010-mRNA-1
chr1_4	exon	69092075	69092191	69092075	ID=Msa0181010-mRNA-1:exon:22940;Parent=Msa0181010-mRNA-1
chr1_4	exon	69092413	69092505	69092413	ID=Msa0181010-mRNA-1:exon:22941;Parent=Msa0181010-mRNA-1
chr1_4	exon	69092609	69092707	69092609	ID=Msa0181010-mRNA-1:exon:22942;Parent=Msa0181010-mRNA-1
chr1_4	exon	69093147	69093826	69093147	ID=Msa0181010-mRNA-1:exon:22943;Parent=Msa0181010-mRNA-1
chr1_4	five_prime_UTR	69090547	69090629	69090547	ID=Msa0181010-mRNA-1:five_prime_utr;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69090630	69090749	69090630	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69090848	69090970	69090848	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69091206	69091280	69091206	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69091385	69091450	69091385	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69091788	69091883	69091788	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69092075	69092191	69092075	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69092413	69092505	69092413	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69092609	69092707	69092609	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	CDS	69093147	69093179	69093147	ID=Msa0181010-mRNA-1:cds;Parent=Msa0181010-mRNA-1
chr1_4	three_prime_UTR	69093180	69093826	69093180	ID=Msa0181010-mRNA-1:three_prime_utr;Parent=Msa0181010-mRNA-1

Gene Sequence

>Msa0181010

ATGGCAGCCTTAAGTTGCCAGTTTCATTCCATCAACTACTTGAATTCTCAAATGGTCAAAAGCAGAAGCAAGCATTCTATAATTTCCTGCTCAGCACAACCTATACAAAGCAATATAAAGGTTGTAATAAATGGAGCAGCAAAGGAAATAGGAAGAGCAGCAGTATTAGCTGTGACAAAGGCCAGAGGAATGGAAGTTGCAGGAGCTATTGATACTCAACATGTTGGGGAAGATATTGGACAGCTTTGTGGCATGGAAGATCCTCTTGAAATACCTATACTAAATGACCTTACCATGGTTTTGGGCTCTATATCTCAGTCCAAGGCCACAGCGGTTGTAGTCGATTTCACTGACCCTTCCGCGGTATATGACAATGTAAAACAGGCAACAGCATTTGGCATGAAAAGTGTTGTTTACGTGCCGCGAATCAAATTAGATACGGTTGCAGCATTATCAGCATTCTGTGAGAAGGCCAGCATGGGTGTCTTGGTTGCTCCAACGCTTTCCATAGGATCAATATTGTTGCAGCAGGCTGCAATTTCAGCTTCTTTCCATTACCGCAATGTCGAAATTGTGGAATCCAAGGCTAATGCAAATGATCTTCCATCGGCCGAAGCAAACCAAATTGCCAACAATCTCTCTAACCTCGGTCAAATCTATAACAGAGAAGACTCCTCAACAGATGTCTTGGCAAGGGGTCAAGTTTTGGGAGATGGAATTCGCGTGCACAGTTTGGTTTTACCAGGGCTTCCCTCTAGTACAACAGTTCACTTTTCTGGTCTTGGAGAGAACTTAGTATACGGCTTGGAGAAGTTTTTGTGA

Protein sequence

>Msa0181010

MAALSCQFHSINYLNSQMVKSRSKHSIISCSAQPIQSNIKVVINGAAKEIGRAAVLAVTKARGMEVAGAIDTQHVGEDIGQLCGMEDPLEIPILNDLTMVLGSISQSKATAVVVDFTDPSAVYDNVKQATAFGMKSVVYVPRIKLDTVAALSAFCEKASMGVLVAPTLSIGSILLQQAAISASFHYRNVEIVESKANANDLPSAEANQIANNLSNLGQIYNREDSSTDVLARGQVLGDGIRVHSLVLPGLPSSTTVHFSGLGENLVYGLEKFL*