AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0582560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582560 MtrunA17_Chr8g0355631 100.000 37 0 0 1 37 35 71 2.22e-20 74.7
Msa0582560 MtrunA17_Chr4g0015761 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa0582560 MtrunA17_CPg0492811 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa0582560 MtrunA17_Chr4g0016861 100.000 35 0 0 1 35 1 35 9.96e-19 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582560 ATCG00600.1 97.222 36 1 0 1 36 1 36 2.23e-18 69.3

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTGAAGTTTCTCTATT+TGG 0.115333 4_2:+1072321 Msa0582560:CDS
TCATGTCAATTGCCAATTCT+TGG 0.245809 4_2:+1072217 Msa0582560:five_prime_UTR
AATAATCCAGCCAAAGTAAT+AGG 0.315680 4_2:-1072363 None:intergenic
AAACTTCAATCATTTGAGTT+TGG 0.328054 4_2:-1072311 None:intergenic
CTAATTCCTATTACTTTGGC+TGG 0.352028 4_2:+1072357 Msa0582560:CDS
TCTCTATTTGGAATCGTGTT+AGG 0.358426 4_2:+1072333 Msa0582560:CDS
AGGTCTAATTCCTATTACTT+TGG 0.388074 4_2:+1072353 Msa0582560:CDS
ATCATTTGAGTTTGGTTGAA+AGG 0.406278 4_2:-1072303 None:intergenic
GAATCTCAATGACCAAGAAT+TGG 0.414372 4_2:-1072229 None:intergenic
AATTGGCAATTGACATGATA+AGG 0.448909 4_2:-1072212 None:intergenic
TTCTTGGTCATTGAGATTCA+CGG 0.471290 4_2:+1072233 Msa0582560:five_prime_UTR
ATATCGACGTGGTGATCAGT+TGG 0.572503 4_2:+1072404 Msa0582560:CDS
TTTGGCTGGATTATTTGTAA+CGG 0.592623 4_2:+1072371 Msa0582560:CDS
GCATATTTACAATATCGACG+TGG 0.789819 4_2:+1072393 Msa0582560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTCAATCATTTGAGTT+TGG - chr4_2:1072314-1072333 None:intergenic 25.0%
! ATGATTGAAGTTTCTCTATT+TGG + chr4_2:1072321-1072340 Msa0582560:CDS 25.0%
AATTGGCAATTGACATGATA+AGG - chr4_2:1072215-1072234 None:intergenic 30.0%
TCAATACAGATTCCTATTGA+AGG + chr4_2:1072256-1072275 Msa0582560:intron 30.0%
GGTAATATCTATCCTTCAAT+AGG - chr4_2:1072271-1072290 None:intergenic 30.0%
TGGTTGAAAGGAAAAAAAGT+GGG - chr4_2:1072294-1072313 None:intergenic 30.0%
TTGGTTGAAAGGAAAAAAAG+TGG - chr4_2:1072295-1072314 None:intergenic 30.0%
! ATCATTTGAGTTTGGTTGAA+AGG - chr4_2:1072306-1072325 None:intergenic 30.0%
AGGTCTAATTCCTATTACTT+TGG + chr4_2:1072353-1072372 Msa0582560:CDS 30.0%
AATAATCCAGCCAAAGTAAT+AGG - chr4_2:1072366-1072385 None:intergenic 30.0%
TTTGGCTGGATTATTTGTAA+CGG + chr4_2:1072371-1072390 Msa0582560:CDS 30.0%
TCATGTCAATTGCCAATTCT+TGG + chr4_2:1072217-1072236 Msa0582560:five_prime_UTR 35.0%
GAATCTCAATGACCAAGAAT+TGG - chr4_2:1072232-1072251 None:intergenic 35.0%
TTCTTGGTCATTGAGATTCA+CGG + chr4_2:1072233-1072252 Msa0582560:five_prime_UTR 35.0%
GTTGAAAGGAAAAAAAGTGG+GGG - chr4_2:1072292-1072311 None:intergenic 35.0%
GGTTGAAAGGAAAAAAAGTG+GGG - chr4_2:1072293-1072312 None:intergenic 35.0%
! TCTCTATTTGGAATCGTGTT+AGG + chr4_2:1072333-1072352 Msa0582560:CDS 35.0%
CTAATTCCTATTACTTTGGC+TGG + chr4_2:1072357-1072376 Msa0582560:CDS 35.0%
GCATATTTACAATATCGACG+TGG + chr4_2:1072393-1072412 Msa0582560:CDS 35.0%
ATATCGACGTGGTGATCAGT+TGG + chr4_2:1072404-1072423 Msa0582560:CDS 45.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 1072210 1072431 1072210 ID=Msa0582560;Name=Msa0582560
chr4_2 mRNA 1072210 1072431 1072210 ID=Msa0582560-mRNA-1;Parent=Msa0582560;Name=Msa0582560-mRNA-1;_AED=0.42;_eAED=0.67;_QI=83|0|0|1|0|0|2|0|37
chr4_2 exon 1072210 1072254 1072210 ID=Msa0582560-mRNA-1:exon:220;Parent=Msa0582560-mRNA-1
chr4_2 exon 1072283 1072431 1072283 ID=Msa0582560-mRNA-1:exon:221;Parent=Msa0582560-mRNA-1
chr4_2 five_prime_UTR 1072210 1072254 1072210 ID=Msa0582560-mRNA-1:five_prime_utr;Parent=Msa0582560-mRNA-1
chr4_2 five_prime_UTR 1072283 1072320 1072283 ID=Msa0582560-mRNA-1:five_prime_utr;Parent=Msa0582560-mRNA-1
chr4_2 CDS 1072321 1072431 1072321 ID=Msa0582560-mRNA-1:cds;Parent=Msa0582560-mRNA-1
Gene Sequence

>Msa0582560

ATGATTGAAGTTTCTCTATTTGGAATCGTGTTAGGTCTAATTCCTATTACTTTGGCTGGATTATTTGTAACGGCATATTTACAATATCGACGTGGTGATCAGTTGGATATT

Protein sequence

>Msa0582560

MIEVSLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDI