AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086840 MtrunA17_Chr8g0355631 100.000 37 0 0 1 37 35 71 2.22e-20 74.7
Msa1086840 MtrunA17_Chr4g0015761 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa1086840 MtrunA17_CPg0492811 100.000 37 0 0 1 37 35 71 2.56e-20 74.7
Msa1086840 MtrunA17_Chr4g0016861 100.000 35 0 0 1 35 1 35 9.96e-19 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086840 ATCG00600.1 97.222 36 1 0 1 36 1 36 2.23e-18 69.3

Find 14 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTGAAGTTTCTCTATT+TGG 0.115333 7_2:-87593440 Msa1086840:CDS
TCATGTCAATTGCCAATTCT+TGG 0.245809 7_2:-87593544 Msa1086840:five_prime_UTR
AATAATCCAGCCAAAGTAAT+AGG 0.315680 7_2:+87593398 None:intergenic
AAACTTCAATCATTTGAGTT+TGG 0.328054 7_2:+87593450 None:intergenic
CTAATTCCTATTACTTTGGC+TGG 0.352028 7_2:-87593404 Msa1086840:CDS
TCTCTATTTGGAATCGTGTT+AGG 0.358426 7_2:-87593428 Msa1086840:CDS
AGGTCTAATTCCTATTACTT+TGG 0.388074 7_2:-87593408 Msa1086840:CDS
ATCATTTGAGTTTGGTTGAA+AGG 0.406278 7_2:+87593458 None:intergenic
GAATCTCAATGACCAAGAAT+TGG 0.414372 7_2:+87593532 None:intergenic
AATTGGCAATTGACATGATA+AGG 0.448909 7_2:+87593549 None:intergenic
TTCTTGGTCATTGAGATTCA+CGG 0.471290 7_2:-87593528 Msa1086840:intron
ATATCGACGTGGTGATCAGT+TGG 0.572503 7_2:-87593357 Msa1086840:CDS
TTTGGCTGGATTATTTGTAA+CGG 0.592623 7_2:-87593390 Msa1086840:CDS
GCATATTTACAATATCGACG+TGG 0.789819 7_2:-87593368 Msa1086840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTCAATCATTTGAGTT+TGG + chr7_2:87593456-87593475 None:intergenic 25.0%
! ATGATTGAAGTTTCTCTATT+TGG - chr7_2:87593463-87593482 Msa1086840:five_prime_UTR 25.0%
AATTGGCAATTGACATGATA+AGG + chr7_2:87593357-87593376 None:intergenic 30.0%
TCAATACAGATTCCTATTGA+AGG - chr7_2:87593398-87593417 Msa1086840:CDS 30.0%
GGTAATATCTATCCTTCAAT+AGG + chr7_2:87593413-87593432 None:intergenic 30.0%
TGGTTGAAAGGAAAAAAAGT+GGG + chr7_2:87593436-87593455 None:intergenic 30.0%
TTGGTTGAAAGGAAAAAAAG+TGG + chr7_2:87593437-87593456 None:intergenic 30.0%
! ATCATTTGAGTTTGGTTGAA+AGG + chr7_2:87593448-87593467 None:intergenic 30.0%
AGGTCTAATTCCTATTACTT+TGG - chr7_2:87593495-87593514 Msa1086840:intron 30.0%
AATAATCCAGCCAAAGTAAT+AGG + chr7_2:87593508-87593527 None:intergenic 30.0%
TTTGGCTGGATTATTTGTAA+CGG - chr7_2:87593513-87593532 Msa1086840:intron 30.0%
TCATGTCAATTGCCAATTCT+TGG - chr7_2:87593359-87593378 Msa1086840:CDS 35.0%
GAATCTCAATGACCAAGAAT+TGG + chr7_2:87593374-87593393 None:intergenic 35.0%
TTCTTGGTCATTGAGATTCA+CGG - chr7_2:87593375-87593394 Msa1086840:CDS 35.0%
GTTGAAAGGAAAAAAAGTGG+GGG + chr7_2:87593434-87593453 None:intergenic 35.0%
GGTTGAAAGGAAAAAAAGTG+GGG + chr7_2:87593435-87593454 None:intergenic 35.0%
! TCTCTATTTGGAATCGTGTT+AGG - chr7_2:87593475-87593494 Msa1086840:five_prime_UTR 35.0%
CTAATTCCTATTACTTTGGC+TGG - chr7_2:87593499-87593518 Msa1086840:intron 35.0%
GCATATTTACAATATCGACG+TGG - chr7_2:87593535-87593554 Msa1086840:five_prime_UTR 35.0%
ATATCGACGTGGTGATCAGT+TGG - chr7_2:87593546-87593565 Msa1086840:five_prime_UTR 45.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87593352 87593573 87593352 ID=Msa1086840;Name=Msa1086840
chr7_2 mRNA 87593352 87593573 87593352 ID=Msa1086840-mRNA-1;Parent=Msa1086840;Name=Msa1086840-mRNA-1;_AED=0.42;_eAED=0.67;_QI=83|0|0|1|0|0|2|0|37
chr7_2 exon 87593529 87593573 87593529 ID=Msa1086840-mRNA-1:exon:23966;Parent=Msa1086840-mRNA-1
chr7_2 exon 87593352 87593500 87593352 ID=Msa1086840-mRNA-1:exon:23965;Parent=Msa1086840-mRNA-1
chr7_2 five_prime_UTR 87593529 87593573 87593529 ID=Msa1086840-mRNA-1:five_prime_utr;Parent=Msa1086840-mRNA-1
chr7_2 five_prime_UTR 87593463 87593500 87593463 ID=Msa1086840-mRNA-1:five_prime_utr;Parent=Msa1086840-mRNA-1
chr7_2 CDS 87593352 87593462 87593352 ID=Msa1086840-mRNA-1:cds;Parent=Msa1086840-mRNA-1
Gene Sequence

>Msa1086840

ATGATTGAAGTTTCTCTATTTGGAATCGTGTTAGGTCTAATTCCTATTACTTTGGCTGGATTATTTGTAACGGCATATTTACAATATCGACGTGGTGATCAGTTGGATATT

Protein sequence

>Msa1086840

MIEVSLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDI