AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0986560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTGATTGCAAAACATTTC+AGG 0.237640 6_4:-35230950 Msa0986560:five_prime_UTR
CCGAGTACAGTATTTGATTC+TGG 0.326284 6_4:+35231332 None:intergenic
GTACCTTCCGTCAATGTCAT+AGG 0.369451 6_4:-35231377 Msa0986560:five_prime_UTR
AATAATTGCTGCTGGGATTC+CGG 0.377025 6_4:+35231097 None:intergenic
ATTTCAATTGCCAGCTAACT+TGG 0.391080 6_4:-35231077 Msa0986560:five_prime_UTR
ATATGTCACAATGATAGATT+TGG 0.405859 6_4:-35230903 Msa0986560:exon
TATATTAACTTAAGAAAAGA+TGG 0.415964 6_4:+35230859 None:intergenic
GTCTCTCCCTATGACATTGA+CGG 0.434125 6_4:+35231370 None:intergenic
CTGCTGCGAGAACTATCAAA+AGG 0.443736 6_4:-35231033 Msa0986560:five_prime_UTR
AACTGTTGAACCAAGTTAGC+TGG 0.464113 6_4:+35231067 None:intergenic
GCAATTGAAATAATTGCTGC+TGG 0.469883 6_4:+35231089 None:intergenic
TACAGTATTTGATTCTGGTC+GGG 0.473562 6_4:+35231337 None:intergenic
AACAGGAAGAATGTCTTGAA+TGG 0.494577 6_4:+35231276 None:intergenic
TCAGGAATGACGGAAAGAAC+AGG 0.508934 6_4:+35231259 None:intergenic
GATAGTTCTCGCAGCAGCGC+GGG 0.517491 6_4:+35231039 None:intergenic
CCAGAATCAAATACTGTACT+CGG 0.551398 6_4:-35231332 Msa0986560:five_prime_UTR
TACCTTCCGTCAATGTCATA+GGG 0.560231 6_4:-35231376 Msa0986560:five_prime_UTR
TAGATTTACCTCAGGAATGA+CGG 0.561552 6_4:+35231249 None:intergenic
GTACAGTATTTGATTCTGGT+CGG 0.577930 6_4:+35231336 None:intergenic
TGTTCTTTCCGTCATTCCTG+AGG 0.588868 6_4:-35231257 Msa0986560:intron
CAATTGAAATAATTGCTGCT+GGG 0.591793 6_4:+35231090 None:intergenic
TGCTGCGAGAACTATCAAAA+GGG 0.593150 6_4:-35231032 Msa0986560:five_prime_UTR
AATTGCTGCTGGGATTCCGG+TGG 0.620635 6_4:+35231100 None:intergenic
ATGTAAATTTCAGCTCCCAC+CGG 0.623839 6_4:-35231116 Msa0986560:intron
CTCCCTATGACATTGACGGA+AGG 0.631184 6_4:+35231374 None:intergenic
ATTGCTGCTGGGATTCCGGT+GGG 0.640904 6_4:+35231101 None:intergenic
AATGTCATAGGGAGAGACAA+AGG 0.665794 6_4:-35231365 Msa0986560:five_prime_UTR
TGATAGTTCTCGCAGCAGCG+CGG 0.673188 6_4:+35231038 None:intergenic
GAATCAAATACTGTACTCGG+AGG 0.741867 6_4:-35231329 Msa0986560:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTAACTTAAGAAAAGA+TGG + chr6_4:35231292-35231311 None:intergenic 15.0%
!! TACCAAAAAGTAATAAAGAA+TGG - chr6_4:35231389-35231408 Msa0986560:five_prime_UTR 20.0%
!!! TTCCATTCTTTATTACTTTT+TGG + chr6_4:35231394-35231413 None:intergenic 20.0%
!!! TTTTTTTTTGCTTCTCACTA+AGG - chr6_4:35231178-35231197 Msa0986560:intron 25.0%
! ATATGTCACAATGATAGATT+TGG - chr6_4:35231245-35231264 Msa0986560:intron 25.0%
CAATTGAAATAATTGCTGCT+GGG + chr6_4:35231061-35231080 None:intergenic 30.0%
!!! AAAAGGGTGTTTTTGTGATA+TGG - chr6_4:35231132-35231151 Msa0986560:intron 30.0%
! TTTTTGTGATATGGATGTAC+TGG - chr6_4:35231141-35231160 Msa0986560:intron 30.0%
AAAACCAACAGAAAAAGCAA+AGG + chr6_4:35231331-35231350 None:intergenic 30.0%
!! TTGGTTTTCATGTGATGATT+AGG - chr6_4:35231343-35231362 Msa0986560:five_prime_UTR 30.0%
TACAGTATTTGATTCTGGTC+GGG + chr6_4:35230814-35230833 None:intergenic 35.0%
GTACAGTATTTGATTCTGGT+CGG + chr6_4:35230815-35230834 None:intergenic 35.0%
CCAGAATCAAATACTGTACT+CGG - chr6_4:35230816-35230835 Msa0986560:three_prime_UTR 35.0%
AACAGGAAGAATGTCTTGAA+TGG + chr6_4:35230875-35230894 None:intergenic 35.0%
TAGATTTACCTCAGGAATGA+CGG + chr6_4:35230902-35230921 None:intergenic 35.0%
CAAATGCTTAGATTTACCTC+AGG + chr6_4:35230910-35230929 None:intergenic 35.0%
!! AGTTGTTTTGTCAGTGTTAG+TGG + chr6_4:35230954-35230973 None:intergenic 35.0%
GTCATAAGCAATTTCACATC+TGG - chr6_4:35230976-35230995 Msa0986560:five_prime_UTR 35.0%
GCAATTGAAATAATTGCTGC+TGG + chr6_4:35231062-35231081 None:intergenic 35.0%
ATTTCAATTGCCAGCTAACT+TGG - chr6_4:35231071-35231090 Msa0986560:five_prime_UTR 35.0%
AGGTGATTGCAAAACATTTC+AGG - chr6_4:35231198-35231217 Msa0986560:intron 35.0%
!!! TGTACCTTTGCTTTTTCTGT+TGG - chr6_4:35231324-35231343 Msa0986560:five_prime_UTR 35.0%
TACCTTCCGTCAATGTCATA+GGG - chr6_4:35230772-35230791 Msa0986560:three_prime_UTR 40.0%
AATGTCATAGGGAGAGACAA+AGG - chr6_4:35230783-35230802 Msa0986560:three_prime_UTR 40.0%
CCGAGTACAGTATTTGATTC+TGG + chr6_4:35230819-35230838 None:intergenic 40.0%
GAATCAAATACTGTACTCGG+AGG - chr6_4:35230819-35230838 Msa0986560:three_prime_UTR 40.0%
ATGTAAATTTCAGCTCCCAC+CGG - chr6_4:35231032-35231051 Msa0986560:five_prime_UTR 40.0%
AATAATTGCTGCTGGGATTC+CGG + chr6_4:35231054-35231073 None:intergenic 40.0%
AACTGTTGAACCAAGTTAGC+TGG + chr6_4:35231084-35231103 None:intergenic 40.0%
TGCTGCGAGAACTATCAAAA+GGG - chr6_4:35231116-35231135 Msa0986560:intron 40.0%
GTACCTTCCGTCAATGTCAT+AGG - chr6_4:35230771-35230790 Msa0986560:three_prime_UTR 45.0%
GTCTCTCCCTATGACATTGA+CGG + chr6_4:35230781-35230800 None:intergenic 45.0%
! TCAGGAATGACGGAAAGAAC+AGG + chr6_4:35230892-35230911 None:intergenic 45.0%
TGTTCTTTCCGTCATTCCTG+AGG - chr6_4:35230891-35230910 Msa0986560:CDS 45.0%
CTGCTGCGAGAACTATCAAA+AGG - chr6_4:35231115-35231134 Msa0986560:intron 45.0%
CTCCCTATGACATTGACGGA+AGG + chr6_4:35230777-35230796 None:intergenic 50.0%
ATTGCTGCTGGGATTCCGGT+GGG + chr6_4:35231050-35231069 None:intergenic 55.0%
AATTGCTGCTGGGATTCCGG+TGG + chr6_4:35231051-35231070 None:intergenic 55.0%
TGATAGTTCTCGCAGCAGCG+CGG + chr6_4:35231113-35231132 None:intergenic 55.0%
GATAGTTCTCGCAGCAGCGC+GGG + chr6_4:35231112-35231131 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 35230750 35231420 35230750 ID=Msa0986560;Name=Msa0986560
chr6_4 mRNA 35230750 35231420 35230750 ID=Msa0986560-mRNA-1;Parent=Msa0986560;Name=Msa0986560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=373|1|1|1|1|1|2|118|15
chr6_4 exon 35230750 35231125 35230750 ID=Msa0986560-mRNA-1:exon:9021;Parent=Msa0986560-mRNA-1
chr6_4 exon 35231258 35231420 35231258 ID=Msa0986560-mRNA-1:exon:9020;Parent=Msa0986560-mRNA-1
chr6_4 five_prime_UTR 35231258 35231420 35231258 ID=Msa0986560-mRNA-1:five_prime_utr;Parent=Msa0986560-mRNA-1
chr6_4 five_prime_UTR 35230916 35231125 35230916 ID=Msa0986560-mRNA-1:five_prime_utr;Parent=Msa0986560-mRNA-1
chr6_4 CDS 35230868 35230915 35230868 ID=Msa0986560-mRNA-1:cds;Parent=Msa0986560-mRNA-1
chr6_4 three_prime_UTR 35230750 35230867 35230750 ID=Msa0986560-mRNA-1:three_prime_utr;Parent=Msa0986560-mRNA-1
Gene Sequence

>Msa0986560

ATGATAGATTTGGCAAAAAATTCTGTCTTCAATTCCATCTTTTCTTAA

Protein sequence

>Msa0986560

MIDLAKNSVFNSIFS*