Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043020 | XP_003621675.2 | 93.382 | 272 | 16 | 1 | 1 | 270 | 692 | 963 | 7.14e-172 | 509 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043020 | sp|C0LGD7|Y1684_ARATH | 58.545 | 275 | 93 | 3 | 1 | 270 | 695 | 953 | 7.65e-96 | 304 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004340 | Msa1043020 | 0.891605 | 3.409796e-74 | -8.615850e-47 |
| Msa0005690 | Msa1043020 | 0.839068 | 1.976669e-57 | -8.615850e-47 |
| Msa0006050 | Msa1043020 | 0.827511 | 1.502623e-54 | -8.615850e-47 |
| Msa0007570 | Msa1043020 | 0.815626 | 8.393805e-52 | -8.615850e-47 |
| Msa0008250 | Msa1043020 | 0.836827 | 7.447118e-57 | -8.615850e-47 |
| Msa0009530 | Msa1043020 | 0.850259 | 1.906535e-60 | -8.615850e-47 |
| Msa0009560 | Msa1043020 | 0.856964 | 2.252467e-62 | -8.615850e-47 |
| Msa0011060 | Msa1043020 | 0.801189 | 1.012522e-48 | -8.615850e-47 |
| Msa0012380 | Msa1043020 | 0.828470 | 8.833023e-55 | -8.615850e-47 |
| Msa0014790 | Msa1043020 | 0.828480 | 8.784420e-55 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043020 | MtrunA17_Chr7g0221711 | 93.382 | 272 | 16 | 1 | 1 | 270 | 692 | 963 | 6.57e-176 | 509 |
| Msa1043020 | MtrunA17_Chr7g0245221 | 87.500 | 272 | 32 | 2 | 1 | 270 | 691 | 962 | 2.78e-164 | 480 |
| Msa1043020 | MtrunA17_Chr3g0108361 | 62.044 | 274 | 90 | 3 | 1 | 270 | 692 | 955 | 8.22e-108 | 333 |
| Msa1043020 | MtrunA17_Chr5g0432871 | 60.073 | 273 | 95 | 4 | 1 | 270 | 686 | 947 | 7.20e-101 | 315 |
| Msa1043020 | MtrunA17_Chr3g0108351 | 67.290 | 214 | 67 | 1 | 1 | 214 | 690 | 900 | 7.10e-94 | 296 |
| Msa1043020 | MtrunA17_Chr8g0369231 | 49.550 | 222 | 80 | 6 | 1 | 207 | 346 | 550 | 8.72e-56 | 189 |
| Msa1043020 | MtrunA17_Chr8g0369211 | 49.309 | 217 | 90 | 5 | 1 | 208 | 699 | 904 | 5.25e-54 | 187 |
| Msa1043020 | MtrunA17_Chr2g0283111 | 48.148 | 216 | 97 | 5 | 1 | 210 | 681 | 887 | 3.42e-53 | 185 |
| Msa1043020 | MtrunA17_Chr7g0263591 | 45.417 | 240 | 116 | 5 | 1 | 233 | 674 | 905 | 2.61e-52 | 182 |
| Msa1043020 | MtrunA17_Chr1g0181731 | 45.575 | 226 | 109 | 4 | 1 | 219 | 668 | 886 | 3.28e-51 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043020 | AT1G06840.1 | 58.545 | 275 | 93 | 3 | 1 | 270 | 695 | 953 | 7.78e-97 | 304 |
| Msa1043020 | AT5G01950.2 | 66.355 | 214 | 69 | 1 | 1 | 214 | 611 | 821 | 1.03e-93 | 295 |
| Msa1043020 | AT5G01950.3 | 66.355 | 214 | 69 | 1 | 1 | 214 | 696 | 906 | 1.07e-92 | 293 |
| Msa1043020 | AT5G01950.1 | 66.355 | 214 | 69 | 1 | 1 | 214 | 696 | 906 | 1.07e-92 | 293 |
| Msa1043020 | AT5G01950.4 | 66.355 | 214 | 69 | 1 | 1 | 214 | 696 | 906 | 1.07e-92 | 293 |
| Msa1043020 | AT5G01950.8 | 66.355 | 214 | 69 | 1 | 1 | 214 | 696 | 906 | 1.07e-92 | 293 |
| Msa1043020 | AT5G01950.7 | 66.355 | 214 | 69 | 1 | 1 | 214 | 701 | 911 | 1.15e-92 | 293 |
| Msa1043020 | AT5G37450.4 | 52.190 | 274 | 112 | 4 | 1 | 270 | 498 | 756 | 2.20e-86 | 273 |
| Msa1043020 | AT5G37450.1 | 52.190 | 274 | 112 | 4 | 1 | 270 | 677 | 935 | 5.57e-85 | 273 |
| Msa1043020 | AT5G37450.3 | 52.190 | 274 | 112 | 4 | 1 | 270 | 701 | 959 | 6.31e-85 | 273 |
Find 206 sgRNAs with CRISPR-Local
Find 432 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAATGAACTGGGGATG+AGG | 0.490099 | 7_2:-752175 | None:intergenic |
| AAAAGTGATGTATACAGCCT+AGG | 0.574376 | 7_2:+754364 | Msa1043020:CDS |
| AAACAATGAACTGGGGATGA+GGG | 0.566266 | 7_2:-752174 | None:intergenic |
| AAACTCACATTATCACAAGA+TGG | 0.500686 | 7_2:-749411 | None:intergenic |
| AAACTGAGGAACTGGAATAA+GGG | 0.485076 | 7_2:+748302 | Msa1043020:five_prime_UTR |
| AAAGTTGGTGAAGGAGGTTA+TGG | 0.426700 | 7_2:+752969 | Msa1043020:five_prime_UTR |
| AAATTTACAAAGTCCTGTGT+AGG | 0.604474 | 7_2:-755330 | None:intergenic |
| AACAATAATTTCAGCGGAAA+TGG | 0.295176 | 7_2:+750741 | Msa1043020:five_prime_UTR |
| AACAGTTGTGAAGGGAACAC+CGG | 0.646400 | 7_2:+754016 | Msa1043020:CDS |
| AACATCAGGATTGACAAGAA+AGG | 0.553599 | 7_2:+755162 | Msa1043020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATTGCAATGAAAATCAGT+GGG | - | chr7_2:747594-747613 | None:intergenic | 30.0% | |
| AGTATTGCAATGAAAATCAG+TGG | - | chr7_2:747595-747614 | None:intergenic | 30.0% | |
| AAAAATTCATGGATATGCCT+TGG | + | chr7_2:747925-747944 | Msa1043020:five_prime_UTR | 30.0% | |
| TAAAATCGCAATCAAAATCG+AGG | + | chr7_2:748647-748666 | Msa1043020:intron | 30.0% | |
| ATAGAACCAGGTATTCAAAT+TGG | + | chr7_2:749004-749023 | Msa1043020:intron | 30.0% | |
| TAGAACCAGGTATTCAAATT+GGG | + | chr7_2:749005-749024 | Msa1043020:intron | 30.0% | |
| AACTCACATTATCACAAGAT+GGG | - | chr7_2:749413-749432 | None:intergenic | 30.0% | |
| AAACTCACATTATCACAAGA+TGG | - | chr7_2:749414-749433 | None:intergenic | 30.0% | |
| GATGTGACTTTCTTATCTAA+TGG | + | chr7_2:749560-749579 | Msa1043020:intron | 30.0% | |
| CTAACATGTACAAGCAAAAT+AGG | - | chr7_2:749614-749633 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 747552 | 755406 | 747552 | ID=Msa1043020;Name=Msa1043020 |
| chr7_2 | mRNA | 747552 | 755406 | 747552 | ID=Msa1043020-mRNA-1;Parent=Msa1043020;Name=Msa1043020-mRNA-1;_AED=0.16;_eAED=0.16;_QI=2205|1|1|1|0.7|0.76|21|145|270 |
| chr7_2 | exon | 747552 | 747676 | 747552 | ID=Msa1043020-mRNA-1:exon:68;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 747903 | 748007 | 747903 | ID=Msa1043020-mRNA-1:exon:69;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 748257 | 748405 | 748257 | ID=Msa1043020-mRNA-1:exon:70;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 749354 | 749425 | 749354 | ID=Msa1043020-mRNA-1:exon:71;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 749718 | 749789 | 749718 | ID=Msa1043020-mRNA-1:exon:72;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 749960 | 750103 | 749960 | ID=Msa1043020-mRNA-1:exon:73;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 750187 | 750258 | 750187 | ID=Msa1043020-mRNA-1:exon:74;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 750388 | 750459 | 750388 | ID=Msa1043020-mRNA-1:exon:75;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 750731 | 750805 | 750731 | ID=Msa1043020-mRNA-1:exon:76;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 750940 | 751008 | 750940 | ID=Msa1043020-mRNA-1:exon:77;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 751138 | 751206 | 751138 | ID=Msa1043020-mRNA-1:exon:78;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 751308 | 751376 | 751308 | ID=Msa1043020-mRNA-1:exon:79;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 751548 | 751634 | 751548 | ID=Msa1043020-mRNA-1:exon:80;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 751788 | 751856 | 751788 | ID=Msa1043020-mRNA-1:exon:81;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 752017 | 752525 | 752017 | ID=Msa1043020-mRNA-1:exon:82;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 752627 | 752790 | 752627 | ID=Msa1043020-mRNA-1:exon:83;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 752880 | 753162 | 752880 | ID=Msa1043020-mRNA-1:exon:84;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 753329 | 753380 | 753329 | ID=Msa1043020-mRNA-1:exon:85;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 753778 | 754037 | 753778 | ID=Msa1043020-mRNA-1:exon:86;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 754319 | 754453 | 754319 | ID=Msa1043020-mRNA-1:exon:87;Parent=Msa1043020-mRNA-1 |
| chr7_2 | exon | 754896 | 755406 | 754896 | ID=Msa1043020-mRNA-1:exon:88;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 747552 | 747676 | 747552 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 747903 | 748007 | 747903 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 748257 | 748405 | 748257 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 749354 | 749425 | 749354 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 749718 | 749789 | 749718 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 749960 | 750103 | 749960 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 750187 | 750258 | 750187 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 750388 | 750459 | 750388 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 750731 | 750805 | 750731 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 750940 | 751008 | 750940 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 751138 | 751206 | 751138 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 751308 | 751376 | 751308 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 751548 | 751634 | 751548 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 751788 | 751856 | 751788 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 752017 | 752525 | 752017 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 752627 | 752790 | 752627 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | five_prime_UTR | 752880 | 753162 | 752880 | ID=Msa1043020-mRNA-1:five_prime_utr;Parent=Msa1043020-mRNA-1 |
| chr7_2 | CDS | 753329 | 753380 | 753329 | ID=Msa1043020-mRNA-1:cds;Parent=Msa1043020-mRNA-1 |
| chr7_2 | CDS | 753778 | 754037 | 753778 | ID=Msa1043020-mRNA-1:cds;Parent=Msa1043020-mRNA-1 |
| chr7_2 | CDS | 754319 | 754453 | 754319 | ID=Msa1043020-mRNA-1:cds;Parent=Msa1043020-mRNA-1 |
| chr7_2 | CDS | 754896 | 755261 | 754896 | ID=Msa1043020-mRNA-1:cds;Parent=Msa1043020-mRNA-1 |
| chr7_2 | three_prime_UTR | 755262 | 755406 | 755262 | ID=Msa1043020-mRNA-1:three_prime_utr;Parent=Msa1043020-mRNA-1 |
| Gene Sequence |
>Msa1043020 ATGCTGGTTTATGAATTCATGCCCAACGGCACCTTAAGGGAATGGATCTCCGGTAAAAGTAAAAAATGCAATGATGGCCTAAGTTTTTTCCTGAGATTACGGATAGCAATGGATGCAGCCAAAGGCATACTATATCTGCATACAGAGGCTAACCCTCCTGTATACCACAGAGATATTAAAGCCACCAACATACTCCTAGACTCAAAGTTTACAGCTAAAGTGGCTGATTTTGGATTGTCACGACTTGCACCATACTCGGATGAAGAGGGAAATGTACCTAAGTATGTGTCAACAGTTGTGAAGGGAACACCGGGTTACCTGGACCCAGAATATATGATGACTCATATGTTAACTGATAAAAGTGATGTATACAGCCTAGGAATTGTGTTTTTGGAGCTTTTGACCGGCATGCATGCAATATCACGTGGGAAAAACATTGTCCGTGAGGTTAATCTGGCTTGTCGATCTGGTGTAATAGATTCCATTATAGATAACAGAATGGGCGAGTATCCATCTGAATGCTCAGACAAGTTCTTAGCTCTGGCCCTCAGTTGCTGTCATGACCATCCAGATGAGAGACCATCAATGTTTGATGTGGTGAGAGAACTGGAAGACATTATTTCCTTGGTACCTGAAACTGAAATTAGTTTATCGGATGTTTCTTTTGATAATTCTAGTAAAATGGCACCCTCATCTTCATCATCATCAACAACAACATCAGGATTGACAAGAAAGGATCAACAACACATGCCCTCATATGTGTCAGGAAGTGATCTTGTCAGTGATATCATTCCTACTATTGTTCCTCGTTGA |
| Protein sequence |
>Msa1043020 MLVYEFMPNGTLREWISGKSKKCNDGLSFFLRLRIAMDAAKGILYLHTEANPPVYHRDIKATNILLDSKFTAKVADFGLSRLAPYSDEEGNVPKYVSTVVKGTPGYLDPEYMMTHMLTDKSDVYSLGIVFLELLTGMHAISRGKNIVREVNLACRSGVIDSIIDNRMGEYPSECSDKFLALALSCCHDHPDERPSMFDVVRELEDIISLVPETEISLSDVSFDNSSKMAPSSSSSSTTTSGLTRKDQQHMPSYVSGSDLVSDIIPTIVPR* |
