Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1100520 | XP_003610912.2 | 87.755 | 49 | 6 | 0 | 27 | 75 | 628 | 676 | 6.54e-16 | 81.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1100520 | G7K7W5 | 87.755 | 49 | 6 | 0 | 27 | 75 | 628 | 676 | 3.13e-16 | 81.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007850 | Msa1100520 | -0.815051 | 1.126747e-51 | -8.615850e-47 |
| Msa0010770 | Msa1100520 | -0.800468 | 1.421057e-48 | -8.615850e-47 |
| Msa0017320 | Msa1100520 | -0.803885 | 2.816203e-49 | -8.615850e-47 |
| Msa0029260 | Msa1100520 | -0.806955 | 6.395751e-50 | -8.615850e-47 |
| Msa0033880 | Msa1100520 | -0.831650 | 1.480437e-55 | -8.615850e-47 |
| Msa0036370 | Msa1100520 | -0.820410 | 6.960293e-53 | -8.615850e-47 |
| Msa0043500 | Msa1100520 | -0.804585 | 2.012776e-49 | -8.615850e-47 |
| Msa0047250 | Msa1100520 | -0.802028 | 6.811662e-49 | -8.615850e-47 |
| Msa0074750 | Msa1100520 | -0.842136 | 3.109052e-58 | -8.615850e-47 |
| Msa0076780 | Msa1100520 | -0.811718 | 6.084304e-51 | -8.615850e-47 |
| Msa0077900 | Msa1100520 | -0.812471 | 4.170459e-51 | -8.615850e-47 |
| Msa0081650 | Msa1100520 | -0.807940 | 3.953398e-50 | -8.615850e-47 |
| Msa0084430 | Msa1100520 | -0.806239 | 9.060498e-50 | -8.615850e-47 |
| Msa0090030 | Msa1100520 | -0.816633 | 5.000536e-52 | -8.615850e-47 |
| Msa0090390 | Msa1100520 | -0.800533 | 1.378268e-48 | -8.615850e-47 |
| Msa0092170 | Msa1100520 | -0.820691 | 5.999357e-53 | -8.615850e-47 |
| Msa0092440 | Msa1100520 | -0.804858 | 1.764960e-49 | -8.615850e-47 |
| Msa0097950 | Msa1100520 | -0.817123 | 3.881989e-52 | -8.615850e-47 |
| Msa0105520 | Msa1100520 | -0.801418 | 9.089739e-49 | -8.615850e-47 |
| Msa0108870 | Msa1100520 | -0.810272 | 1.251989e-50 | -8.615850e-47 |
| Msa0112300 | Msa1100520 | 0.812165 | 4.864123e-51 | -8.615850e-47 |
| Msa0603670 | Msa1100520 | -0.804222 | 2.395506e-49 | -8.615850e-47 |
| Msa0605000 | Msa1100520 | -0.806423 | 8.287058e-50 | -8.615850e-47 |
| Msa0614510 | Msa1100520 | -0.800938 | 1.139640e-48 | -8.615850e-47 |
| Msa0618200 | Msa1100520 | 0.803486 | 3.407563e-49 | -8.615850e-47 |
| Msa0618930 | Msa1100520 | -0.811744 | 6.008009e-51 | -8.615850e-47 |
| Msa0618950 | Msa1100520 | -0.815843 | 7.508710e-52 | -8.615850e-47 |
| Msa0628420 | Msa1100520 | -0.806458 | 8.146364e-50 | -8.615850e-47 |
| Msa0640940 | Msa1100520 | -0.802884 | 4.539674e-49 | -8.615850e-47 |
| Msa0646130 | Msa1100520 | -0.810461 | 1.139804e-50 | -8.615850e-47 |
| Msa0657460 | Msa1100520 | 0.800108 | 1.682391e-48 | -8.615850e-47 |
| Msa0662630 | Msa1100520 | -0.802162 | 6.392853e-49 | -8.615850e-47 |
| Msa0686080 | Msa1100520 | -0.811992 | 5.304240e-51 | -8.615850e-47 |
| Msa0693790 | Msa1100520 | -0.800429 | 1.447323e-48 | -8.615850e-47 |
| Msa0697720 | Msa1100520 | -0.801122 | 1.045160e-48 | -8.615850e-47 |
| Msa0705390 | Msa1100520 | -0.833141 | 6.324312e-56 | -8.615850e-47 |
| Msa0743530 | Msa1100520 | -0.813726 | 2.211759e-51 | -8.615850e-47 |
| Msa0744100 | Msa1100520 | -0.807405 | 5.136463e-50 | -8.615850e-47 |
| Msa0756930 | Msa1100520 | -0.807573 | 4.732854e-50 | -8.615850e-47 |
| Msa0781270 | Msa1100520 | -0.807362 | 5.246145e-50 | -8.615850e-47 |
| Msa0787150 | Msa1100520 | -0.808117 | 3.626013e-50 | -8.615850e-47 |
| Msa0788120 | Msa1100520 | -0.833364 | 5.565192e-56 | -8.615850e-47 |
| Msa0790170 | Msa1100520 | 0.811361 | 7.275489e-51 | -8.615850e-47 |
| Msa0802410 | Msa1100520 | -0.800813 | 1.208599e-48 | -8.615850e-47 |
| Msa0820530 | Msa1100520 | 0.816298 | 5.943194e-52 | -8.615850e-47 |
| Msa0822170 | Msa1100520 | -0.800021 | 1.752235e-48 | -8.615850e-47 |
| Msa0825270 | Msa1100520 | -0.812301 | 4.542779e-51 | -8.615850e-47 |
| Msa0825280 | Msa1100520 | -0.811648 | 6.301882e-51 | -8.615850e-47 |
| Msa0826150 | Msa1100520 | -0.801133 | 1.039596e-48 | -8.615850e-47 |
| Msa0836500 | Msa1100520 | -0.812071 | 5.097168e-51 | -8.615850e-47 |
| Msa0126880 | Msa1100520 | -0.803398 | 3.553979e-49 | -8.615850e-47 |
| Msa0126910 | Msa1100520 | -0.826731 | 2.309690e-54 | -8.615850e-47 |
| Msa0127040 | Msa1100520 | -0.847918 | 8.531020e-60 | -8.615850e-47 |
| Msa0127850 | Msa1100520 | -0.832550 | 8.866746e-56 | -8.615850e-47 |
| Msa0138210 | Msa1100520 | -0.828978 | 6.654398e-55 | -8.615850e-47 |
| Msa0142500 | Msa1100520 | -0.849298 | 3.538494e-60 | -8.615850e-47 |
| Msa0145150 | Msa1100520 | -0.804460 | 2.136966e-49 | -8.615850e-47 |
| Msa0146080 | Msa1100520 | -0.837713 | 4.419613e-57 | -8.615850e-47 |
| Msa0166750 | Msa1100520 | -0.812298 | 4.548534e-51 | -8.615850e-47 |
| Msa0169300 | Msa1100520 | -0.805109 | 1.564328e-49 | -8.615850e-47 |
| Msa0171980 | Msa1100520 | -0.822682 | 2.078388e-53 | -8.615850e-47 |
| Msa0174000 | Msa1100520 | -0.829948 | 3.867797e-55 | -8.615850e-47 |
| Msa0175290 | Msa1100520 | 0.812414 | 4.291087e-51 | -8.615850e-47 |
| Msa0183870 | Msa1100520 | -0.829166 | 5.992745e-55 | -8.615850e-47 |
| Msa0193600 | Msa1100520 | -0.801057 | 1.077249e-48 | -8.615850e-47 |
| Msa0233370 | Msa1100520 | -0.813379 | 2.637533e-51 | -8.615850e-47 |
| Msa0236070 | Msa1100520 | -0.804380 | 2.221650e-49 | -8.615850e-47 |
| Msa0370160 | Msa1100520 | 0.806228 | 9.110444e-50 | -8.615850e-47 |
| Msa0379260 | Msa1100520 | -0.806003 | 1.015606e-49 | -8.615850e-47 |
| Msa0381000 | Msa1100520 | -0.808353 | 3.228354e-50 | -8.615850e-47 |
| Msa0381080 | Msa1100520 | -0.805933 | 1.050891e-49 | -8.615850e-47 |
| Msa0387050 | Msa1100520 | -0.810745 | 9.892102e-51 | -8.615850e-47 |
| Msa0387880 | Msa1100520 | 0.815515 | 8.888059e-52 | -8.615850e-47 |
| Msa0387910 | Msa1100520 | -0.812006 | 5.267495e-51 | -8.615850e-47 |
| Msa0392200 | Msa1100520 | -0.806333 | 8.654671e-50 | -8.615850e-47 |
| Msa0419520 | Msa1100520 | -0.808044 | 3.758315e-50 | -8.615850e-47 |
| Msa0430870 | Msa1100520 | -0.800552 | 1.365886e-48 | -8.615850e-47 |
| Msa0446900 | Msa1100520 | -0.803163 | 3.973696e-49 | -8.615850e-47 |
| Msa0467260 | Msa1100520 | 0.806335 | 8.649384e-50 | -8.615850e-47 |
| Msa0480780 | Msa1100520 | -0.800419 | 1.454179e-48 | -8.615850e-47 |
| Msa0481280 | Msa1100520 | -0.831072 | 2.054054e-55 | -8.615850e-47 |
| Msa1001350 | Msa1100520 | -0.827744 | 1.320634e-54 | -8.615850e-47 |
| Msa1025940 | Msa1100520 | -0.809763 | 1.611483e-50 | -8.615850e-47 |
| Msa1033970 | Msa1100520 | -0.801829 | 7.485066e-49 | -8.615850e-47 |
| Msa1036740 | Msa1100520 | -0.818850 | 1.580719e-52 | -8.615850e-47 |
| Msa1048750 | Msa1100520 | 0.995101 | 5.043012e-213 | -8.615850e-47 |
| Msa1048760 | Msa1100520 | 0.853006 | 3.177230e-61 | -8.615850e-47 |
| Msa1070390 | Msa1100520 | -0.849786 | 2.585927e-60 | -8.615850e-47 |
| Msa1072710 | Msa1100520 | -0.803608 | 3.214475e-49 | -8.615850e-47 |
| Msa1088250 | Msa1100520 | 0.815037 | 1.134753e-51 | -8.615850e-47 |
| Msa1088290 | Msa1100520 | -0.800411 | 1.459342e-48 | -8.615850e-47 |
| Msa1100520 | Msa1100530 | 0.883359 | 4.796165e-71 | -8.615850e-47 |
| Msa1100520 | Msa1113590 | -0.800443 | 1.437980e-48 | -8.615850e-47 |
| Msa1100520 | Msa1122780 | -0.803239 | 3.833358e-49 | -8.615850e-47 |
| Msa1100520 | Msa1127430 | -0.818956 | 1.495589e-52 | -8.615850e-47 |
| Msa1100520 | Msa1127540 | -0.801804 | 7.573256e-49 | -8.615850e-47 |
| Msa1100520 | Msa1144040 | -0.827496 | 1.515156e-54 | -8.615850e-47 |
| Msa1100520 | Msa1154490 | -0.801458 | 8.919614e-49 | -8.615850e-47 |
| Msa1100520 | Msa1158230 | -0.838038 | 3.646569e-57 | -8.615850e-47 |
| Msa1100520 | Msa1163870 | -0.830260 | 3.245264e-55 | -8.615850e-47 |
| Msa1100520 | Msa1172130 | 0.804277 | 2.334051e-49 | -8.615850e-47 |
| Msa1100520 | Msa1176980 | -0.846549 | 2.025615e-59 | -8.615850e-47 |
| Msa1100520 | Msa1178050 | -0.827241 | 1.743942e-54 | -8.615850e-47 |
| Msa1100520 | Msa1199710 | -0.807100 | 5.959961e-50 | -8.615850e-47 |
| Msa1100520 | Msa1201640 | -0.805547 | 1.266188e-49 | -8.615850e-47 |
| Msa1100520 | Msa1201810 | -0.817639 | 2.970812e-52 | -8.615850e-47 |
| Msa1100520 | Msa1201830 | -0.824565 | 7.536313e-54 | -8.615850e-47 |
| Msa1100520 | Msa1205480 | -0.816490 | 5.384925e-52 | -8.615850e-47 |
| Msa1100520 | Msa1207730 | -0.806348 | 8.592995e-50 | -8.615850e-47 |
| Msa1100520 | Msa1250110 | -0.837457 | 5.138873e-57 | -8.615850e-47 |
| Msa1100520 | Msa1250730 | -0.839540 | 1.491162e-57 | -8.615850e-47 |
| Msa1100520 | Msa1294840 | -0.814564 | 1.444883e-51 | -8.615850e-47 |
| Msa1100520 | Msa1294960 | -0.808034 | 3.775285e-50 | -8.615850e-47 |
| Msa1100520 | Msa1296300 | -0.814352 | 1.609325e-51 | -8.615850e-47 |
| Msa1100520 | Msa1299820 | 0.811653 | 6.286005e-51 | -8.615850e-47 |
| Msa1100520 | Msa1336040 | -0.810144 | 1.333603e-50 | -8.615850e-47 |
| Msa1100520 | Msa1339190 | -0.818146 | 2.281900e-52 | -8.615850e-47 |
| Msa1100520 | Msa1340830 | -0.834761 | 2.486877e-56 | -8.615850e-47 |
| Msa1100520 | Msa1351540 | -0.812437 | 4.241881e-51 | -8.615850e-47 |
| Msa1100520 | Msa1355700 | -0.809610 | 1.737495e-50 | -8.615850e-47 |
| Msa1100520 | Msa1369890 | -0.802034 | 6.795438e-49 | -8.615850e-47 |
| Msa1100520 | Msa1383350 | -0.800420 | 1.453320e-48 | -8.615850e-47 |
| Msa1100520 | Msa1407970 | -0.824108 | 9.649582e-54 | -8.615850e-47 |
| Msa1100520 | Msa1411950 | -0.811260 | 7.651187e-51 | -8.615850e-47 |
| Msa1100520 | Msa1416260 | -0.829992 | 3.773263e-55 | -8.615850e-47 |
| Msa1100520 | Msa1422840 | -0.823586 | 1.278717e-53 | -8.615850e-47 |
| Msa1100520 | Msa1426910 | -0.805571 | 1.251635e-49 | -8.615850e-47 |
| Msa1100520 | Msa1438450 | -0.802008 | 6.876921e-49 | -8.615850e-47 |
| Msa1100520 | Msa1450720 | -0.822993 | 1.759035e-53 | -8.615850e-47 |
| Msa1100520 | Msa1454680 | -0.803609 | 3.212542e-49 | -8.615850e-47 |
| Msa1100520 | Msa1454960 | -0.807536 | 4.818669e-50 | -8.615850e-47 |
| Msa1100520 | Msa1458810 | -0.851439 | 8.866229e-61 | -8.615850e-47 |
| Msa1100520 | Msa1463600 | -0.837880 | 4.003594e-57 | -8.615850e-47 |
| Msa0244970 | Msa1100520 | -0.827145 | 1.839350e-54 | -8.615850e-47 |
| Msa0244980 | Msa1100520 | -0.814556 | 1.450525e-51 | -8.615850e-47 |
| Msa0262480 | Msa1100520 | -0.814845 | 1.252115e-51 | -8.615850e-47 |
| Msa0269520 | Msa1100520 | -0.809903 | 1.503510e-50 | -8.615850e-47 |
| Msa0273650 | Msa1100520 | -0.800321 | 1.522728e-48 | -8.615850e-47 |
| Msa0275360 | Msa1100520 | -0.800474 | 1.416861e-48 | -8.615850e-47 |
| Msa0283420 | Msa1100520 | -0.802477 | 5.507624e-49 | -8.615850e-47 |
| Msa0296980 | Msa1100520 | 0.844898 | 5.683941e-59 | -8.615850e-47 |
| Msa0317640 | Msa1100520 | -0.804720 | 1.886561e-49 | -8.615850e-47 |
| Msa0321690 | Msa1100520 | -0.820381 | 7.067596e-53 | -8.615850e-47 |
| Msa0324240 | Msa1100520 | -0.811699 | 6.144878e-51 | -8.615850e-47 |
| Msa0335320 | Msa1100520 | 0.843439 | 1.399962e-58 | -8.615850e-47 |
| Msa0343190 | Msa1100520 | -0.808260 | 3.379490e-50 | -8.615850e-47 |
| Msa0346990 | Msa1100520 | -0.803726 | 3.037318e-49 | -8.615850e-47 |
| Msa0510290 | Msa1100520 | -0.819740 | 9.908456e-53 | -8.615850e-47 |
| Msa0511490 | Msa1100520 | -0.802438 | 5.610569e-49 | -8.615850e-47 |
| Msa0517090 | Msa1100520 | -0.801603 | 8.328035e-49 | -8.615850e-47 |
| Msa0518960 | Msa1100520 | -0.814683 | 1.359687e-51 | -8.615850e-47 |
| Msa0524590 | Msa1100520 | -0.801948 | 7.076511e-49 | -8.615850e-47 |
| Msa0531250 | Msa1100520 | -0.818123 | 2.309827e-52 | -8.615850e-47 |
| Msa0534410 | Msa1100520 | -0.820067 | 8.343784e-53 | -8.615850e-47 |
| Msa0550570 | Msa1100520 | -0.812136 | 4.934771e-51 | -8.615850e-47 |
| Msa0554290 | Msa1100520 | -0.814022 | 1.903668e-51 | -8.615850e-47 |
| Msa0560500 | Msa1100520 | -0.810973 | 8.830323e-51 | -8.615850e-47 |
| Msa0565620 | Msa1100520 | 0.817307 | 3.529376e-52 | -8.615850e-47 |
| Msa0569780 | Msa1100520 | -0.801025 | 1.093629e-48 | -8.615850e-47 |
| Msa0578090 | Msa1100520 | -0.803945 | 2.736107e-49 | -8.615850e-47 |
| Msa0588090 | Msa1100520 | -0.806206 | 9.205528e-50 | -8.615850e-47 |
| Msa0588120 | Msa1100520 | -0.814277 | 1.672428e-51 | -8.615850e-47 |
| Msa0590650 | Msa1100520 | -0.800581 | 1.347971e-48 | -8.615850e-47 |
| Msa0594200 | Msa1100520 | -0.816486 | 5.393916e-52 | -8.615850e-47 |
| Msa0844230 | Msa1100520 | -0.808382 | 3.183786e-50 | -8.615850e-47 |
| Msa0850710 | Msa1100520 | -0.824887 | 6.325212e-54 | -8.615850e-47 |
| Msa0851470 | Msa1100520 | -0.804986 | 1.660144e-49 | -8.615850e-47 |
| Msa0859180 | Msa1100520 | -0.801318 | 9.529208e-49 | -8.615850e-47 |
| Msa0861830 | Msa1100520 | -0.810088 | 1.371638e-50 | -8.615850e-47 |
| Msa0869010 | Msa1100520 | -0.811399 | 7.137392e-51 | -8.615850e-47 |
| Msa0880040 | Msa1100520 | -0.827609 | 1.423158e-54 | -8.615850e-47 |
| Msa0901450 | Msa1100520 | -0.809854 | 1.539859e-50 | -8.615850e-47 |
| Msa0945240 | Msa1100520 | -0.800331 | 1.515374e-48 | -8.615850e-47 |
| Msa0947800 | Msa1100520 | -0.804064 | 2.584685e-49 | -8.615850e-47 |
| Msa0957870 | Msa1100520 | -0.811134 | 8.147361e-51 | -8.615850e-47 |
| Msa0957970 | Msa1100520 | -0.817718 | 2.852073e-52 | -8.615850e-47 |
| Msa0968440 | Msa1100520 | -0.814390 | 1.578691e-51 | -8.615850e-47 |
| Msa0970530 | Msa1100520 | -0.817642 | 2.966403e-52 | -8.615850e-47 |
| Msa0970600 | Msa1100520 | -0.847246 | 1.306043e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1100520 | MtrunA17_Chr5g0396101 | 87.755 | 49 | 6 | 0 | 27 | 75 | 275 | 323 | 9.76e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCACCTGGTTGCCCATAAC+CGG | 0.242390 | 7_3:+30416286 | None:intergenic |
| CCTGGTGGACAACCCGGTTA+TGG | 0.315095 | 7_3:-30416299 | Msa1100520:CDS |
| CGCATAACTAGGTGCGGCCT+GGG | 0.374054 | 7_3:+30416265 | None:intergenic |
| CTGGTGGACAACCCGGTTAT+GGG | 0.385117 | 7_3:-30416298 | Msa1100520:CDS |
| GCGCATAACTAGGTGCGGCC+TGG | 0.388775 | 7_3:+30416264 | None:intergenic |
| CAAGCAGCATATCCTACATA+TGG | 0.393896 | 7_3:-30416393 | Msa1100520:CDS |
| TATGCGCAAGTTGGTCATAC+TGG | 0.399918 | 7_3:-30416248 | Msa1100520:CDS |
| AACCGGGTTGTCCACCAGGC+TGG | 0.415446 | 7_3:+30416303 | None:intergenic |
| CTATATCTATATGCATCATC+GGG | 0.416909 | 7_3:-30416423 | Msa1100520:CDS |
| CAAGTTGGTCATACTGGTTA+TGG | 0.420943 | 7_3:-30416242 | Msa1100520:CDS |
| CTAGGTGCGGCCTGGGCACC+TGG | 0.421749 | 7_3:+30416272 | None:intergenic |
| CCATAACCGGGTTGTCCACC+AGG | 0.453267 | 7_3:+30416299 | None:intergenic |
| ACTATATCTATATGCATCAT+CGG | 0.493050 | 7_3:-30416424 | Msa1100520:CDS |
| ACCGGGTTGTCCACCAGGCT+GGG | 0.494134 | 7_3:+30416304 | None:intergenic |
| GGCTGGGCATAAACTTGTCC+AGG | 0.498525 | 7_3:+30416320 | None:intergenic |
| TGACCAACTTGCGCATAACT+AGG | 0.503715 | 7_3:+30416254 | None:intergenic |
| AATCACCCGCAGTCTGCACC+TGG | 0.524809 | 7_3:-30416338 | Msa1100520:CDS |
| TTATGGGCAACCAGGTGCCC+AGG | 0.525214 | 7_3:-30416282 | Msa1100520:CDS |
| CTTGTCCAGGTGCAGACTGC+GGG | 0.527053 | 7_3:+30416333 | None:intergenic |
| GCCCAGCCTGGTGGACAACC+CGG | 0.535827 | 7_3:-30416305 | Msa1100520:CDS |
| GCACCTGGTTGCCCATAACC+GGG | 0.540537 | 7_3:+30416287 | None:intergenic |
| GGACAAGTTTATGCCCAGCC+TGG | 0.546505 | 7_3:-30416317 | Msa1100520:CDS |
| CACTCTGCACTAGCTGCCGA+TGG | 0.553366 | 7_3:-30416365 | Msa1100520:CDS |
| CAACCCGGTTATGGGCAACC+AGG | 0.556358 | 7_3:-30416290 | Msa1100520:CDS |
| AAGCAGCATATCCTACATAT+GGG | 0.567067 | 7_3:-30416392 | Msa1100520:CDS |
| CAGAGTGCAGACCCATATGT+AGG | 0.574828 | 7_3:+30416381 | None:intergenic |
| CAAGTTTATGCCCAGCCTGG+TGG | 0.621083 | 7_3:-30416314 | Msa1100520:CDS |
| CTGCGGGTGATTGTAACCAT+CGG | 0.627649 | 7_3:+30416349 | None:intergenic |
| GCACCTAGTTATGCGCAAGT+TGG | 0.628557 | 7_3:-30416257 | Msa1100520:CDS |
| ACTTGTCCAGGTGCAGACTG+CGG | 0.644028 | 7_3:+30416332 | None:intergenic |
| AACTTGCGCATAACTAGGTG+CGG | 0.662539 | 7_3:+30416259 | None:intergenic |
| TATATCTATATGCATCATCG+GGG | 0.741642 | 7_3:-30416422 | Msa1100520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTATATCTATATGCATCAT+CGG | - | chr7_3:30416251-30416270 | Msa1100520:CDS | 25.0% |
| CTATATCTATATGCATCATC+GGG | - | chr7_3:30416252-30416271 | Msa1100520:CDS | 30.0% | |
| TATATCTATATGCATCATCG+GGG | - | chr7_3:30416253-30416272 | Msa1100520:CDS | 30.0% | |
| AAGCAGCATATCCTACATAT+GGG | - | chr7_3:30416283-30416302 | Msa1100520:CDS | 35.0% | |
| CAAGCAGCATATCCTACATA+TGG | - | chr7_3:30416282-30416301 | Msa1100520:CDS | 40.0% | |
| !! | CAAGTTGGTCATACTGGTTA+TGG | - | chr7_3:30416433-30416452 | Msa1100520:CDS | 40.0% |
| AACTTGCGCATAACTAGGTG+CGG | + | chr7_3:30416419-30416438 | None:intergenic | 45.0% | |
| TGACCAACTTGCGCATAACT+AGG | + | chr7_3:30416424-30416443 | None:intergenic | 45.0% | |
| !! | TATGCGCAAGTTGGTCATAC+TGG | - | chr7_3:30416427-30416446 | Msa1100520:CDS | 45.0% |
| CAGAGTGCAGACCCATATGT+AGG | + | chr7_3:30416297-30416316 | None:intergenic | 50.0% | |
| CTGCGGGTGATTGTAACCAT+CGG | + | chr7_3:30416329-30416348 | None:intergenic | 50.0% | |
| GCACCTAGTTATGCGCAAGT+TGG | - | chr7_3:30416418-30416437 | Msa1100520:CDS | 50.0% | |
| ACTTGTCCAGGTGCAGACTG+CGG | + | chr7_3:30416346-30416365 | None:intergenic | 55.0% | |
| GGCTGGGCATAAACTTGTCC+AGG | + | chr7_3:30416358-30416377 | None:intergenic | 55.0% | |
| GGACAAGTTTATGCCCAGCC+TGG | - | chr7_3:30416358-30416377 | Msa1100520:CDS | 55.0% | |
| CAAGTTTATGCCCAGCCTGG+TGG | - | chr7_3:30416361-30416380 | Msa1100520:CDS | 55.0% | |
| ! | CTGGTGGACAACCCGGTTAT+GGG | - | chr7_3:30416377-30416396 | Msa1100520:CDS | 55.0% |
| CACTCTGCACTAGCTGCCGA+TGG | - | chr7_3:30416310-30416329 | Msa1100520:CDS | 60.0% | |
| AATCACCCGCAGTCTGCACC+TGG | - | chr7_3:30416337-30416356 | Msa1100520:CDS | 60.0% | |
| CTTGTCCAGGTGCAGACTGC+GGG | + | chr7_3:30416345-30416364 | None:intergenic | 60.0% | |
| CCATAACCGGGTTGTCCACC+AGG | + | chr7_3:30416379-30416398 | None:intergenic | 60.0% | |
| ! | CCTGGTGGACAACCCGGTTA+TGG | - | chr7_3:30416376-30416395 | Msa1100520:CDS | 60.0% |
| CAACCCGGTTATGGGCAACC+AGG | - | chr7_3:30416385-30416404 | Msa1100520:CDS | 60.0% | |
| GCACCTGGTTGCCCATAACC+GGG | + | chr7_3:30416391-30416410 | None:intergenic | 60.0% | |
| GGCACCTGGTTGCCCATAAC+CGG | + | chr7_3:30416392-30416411 | None:intergenic | 60.0% | |
| TTATGGGCAACCAGGTGCCC+AGG | - | chr7_3:30416393-30416412 | Msa1100520:CDS | 60.0% | |
| CGCATAACTAGGTGCGGCCT+GGG | + | chr7_3:30416413-30416432 | None:intergenic | 60.0% | |
| ACCGGGTTGTCCACCAGGCT+GGG | + | chr7_3:30416374-30416393 | None:intergenic | 65.0% | |
| AACCGGGTTGTCCACCAGGC+TGG | + | chr7_3:30416375-30416394 | None:intergenic | 65.0% | |
| GCGCATAACTAGGTGCGGCC+TGG | + | chr7_3:30416414-30416433 | None:intergenic | 65.0% | |
| ! | GCCCAGCCTGGTGGACAACC+CGG | - | chr7_3:30416370-30416389 | Msa1100520:CDS | 70.0% |
| CTAGGTGCGGCCTGGGCACC+TGG | + | chr7_3:30416406-30416425 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 30416235 | 30416462 | 30416235 | ID=Msa1100520;Name=Msa1100520 |
| chr7_3 | mRNA | 30416235 | 30416462 | 30416235 | ID=Msa1100520-mRNA-1;Parent=Msa1100520;Name=Msa1100520-mRNA-1;_AED=0.46;_eAED=0.64;_QI=0|-1|0|1|-1|1|1|0|75 |
| chr7_3 | exon | 30416235 | 30416462 | 30416235 | ID=Msa1100520-mRNA-1:exon:6475;Parent=Msa1100520-mRNA-1 |
| chr7_3 | CDS | 30416235 | 30416462 | 30416235 | ID=Msa1100520-mRNA-1:cds;Parent=Msa1100520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
