AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1048750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1048750 MtrunA17_Chr5g0396101 87.755 49 6 0 27 75 275 323 9.76e-19 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCACCTGGTTGCCCATAAC+CGG 0.242390 7_2:+10640131 None:intergenic
CCTGGTGGACAACCCGGTTA+TGG 0.315095 7_2:-10640144 Msa1048750:CDS
CGCATAACTAGGTGCGGCCT+GGG 0.374054 7_2:+10640110 None:intergenic
CTGGTGGACAACCCGGTTAT+GGG 0.385117 7_2:-10640143 Msa1048750:CDS
GCGCATAACTAGGTGCGGCC+TGG 0.388775 7_2:+10640109 None:intergenic
CAAGCAGCATATCCTACATA+TGG 0.393896 7_2:-10640238 Msa1048750:CDS
TATGCGCAAGTTGGTCATAC+TGG 0.399918 7_2:-10640093 Msa1048750:CDS
AACCGGGTTGTCCACCAGGC+TGG 0.415446 7_2:+10640148 None:intergenic
CTATATCTATATGCATCATC+GGG 0.416909 7_2:-10640268 Msa1048750:CDS
CAAGTTGGTCATACTGGTTA+TGG 0.420943 7_2:-10640087 Msa1048750:CDS
CTAGGTGCGGCCTGGGCACC+TGG 0.421749 7_2:+10640117 None:intergenic
CCATAACCGGGTTGTCCACC+AGG 0.453267 7_2:+10640144 None:intergenic
ACTATATCTATATGCATCAT+CGG 0.493050 7_2:-10640269 Msa1048750:CDS
ACCGGGTTGTCCACCAGGCT+GGG 0.494134 7_2:+10640149 None:intergenic
GGCTGGGCATAAACTTGTCC+AGG 0.498525 7_2:+10640165 None:intergenic
TGACCAACTTGCGCATAACT+AGG 0.503715 7_2:+10640099 None:intergenic
AATCACCCGCAGTCTGCACC+TGG 0.524809 7_2:-10640183 Msa1048750:CDS
TTATGGGCAACCAGGTGCCC+AGG 0.525214 7_2:-10640127 Msa1048750:CDS
CTTGTCCAGGTGCAGACTGC+GGG 0.527053 7_2:+10640178 None:intergenic
GCCCAGCCTGGTGGACAACC+CGG 0.535827 7_2:-10640150 Msa1048750:CDS
GCACCTGGTTGCCCATAACC+GGG 0.540537 7_2:+10640132 None:intergenic
GGACAAGTTTATGCCCAGCC+TGG 0.546505 7_2:-10640162 Msa1048750:CDS
CACTCTGCACTAGCTGCCGA+TGG 0.553366 7_2:-10640210 Msa1048750:CDS
CAACCCGGTTATGGGCAACC+AGG 0.556358 7_2:-10640135 Msa1048750:CDS
AAGCAGCATATCCTACATAT+GGG 0.567067 7_2:-10640237 Msa1048750:CDS
CAGAGTGCAGACCCATATGT+AGG 0.574828 7_2:+10640226 None:intergenic
CAAGTTTATGCCCAGCCTGG+TGG 0.621083 7_2:-10640159 Msa1048750:CDS
CTGCGGGTGATTGTAACCAT+CGG 0.627649 7_2:+10640194 None:intergenic
GCACCTAGTTATGCGCAAGT+TGG 0.628557 7_2:-10640102 Msa1048750:CDS
ACTTGTCCAGGTGCAGACTG+CGG 0.644028 7_2:+10640177 None:intergenic
AACTTGCGCATAACTAGGTG+CGG 0.662539 7_2:+10640104 None:intergenic
TATATCTATATGCATCATCG+GGG 0.741642 7_2:-10640267 Msa1048750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTATATCTATATGCATCAT+CGG - chr7_2:10640096-10640115 Msa1048750:CDS 25.0%
CTATATCTATATGCATCATC+GGG - chr7_2:10640097-10640116 Msa1048750:CDS 30.0%
TATATCTATATGCATCATCG+GGG - chr7_2:10640098-10640117 Msa1048750:CDS 30.0%
AAGCAGCATATCCTACATAT+GGG - chr7_2:10640128-10640147 Msa1048750:CDS 35.0%
CAAGCAGCATATCCTACATA+TGG - chr7_2:10640127-10640146 Msa1048750:CDS 40.0%
!! CAAGTTGGTCATACTGGTTA+TGG - chr7_2:10640278-10640297 Msa1048750:CDS 40.0%
AACTTGCGCATAACTAGGTG+CGG + chr7_2:10640264-10640283 None:intergenic 45.0%
TGACCAACTTGCGCATAACT+AGG + chr7_2:10640269-10640288 None:intergenic 45.0%
!! TATGCGCAAGTTGGTCATAC+TGG - chr7_2:10640272-10640291 Msa1048750:CDS 45.0%
CAGAGTGCAGACCCATATGT+AGG + chr7_2:10640142-10640161 None:intergenic 50.0%
CTGCGGGTGATTGTAACCAT+CGG + chr7_2:10640174-10640193 None:intergenic 50.0%
GCACCTAGTTATGCGCAAGT+TGG - chr7_2:10640263-10640282 Msa1048750:CDS 50.0%
ACTTGTCCAGGTGCAGACTG+CGG + chr7_2:10640191-10640210 None:intergenic 55.0%
GGCTGGGCATAAACTTGTCC+AGG + chr7_2:10640203-10640222 None:intergenic 55.0%
GGACAAGTTTATGCCCAGCC+TGG - chr7_2:10640203-10640222 Msa1048750:CDS 55.0%
CAAGTTTATGCCCAGCCTGG+TGG - chr7_2:10640206-10640225 Msa1048750:CDS 55.0%
! CTGGTGGACAACCCGGTTAT+GGG - chr7_2:10640222-10640241 Msa1048750:CDS 55.0%
CACTCTGCACTAGCTGCCGA+TGG - chr7_2:10640155-10640174 Msa1048750:CDS 60.0%
AATCACCCGCAGTCTGCACC+TGG - chr7_2:10640182-10640201 Msa1048750:CDS 60.0%
CTTGTCCAGGTGCAGACTGC+GGG + chr7_2:10640190-10640209 None:intergenic 60.0%
CCATAACCGGGTTGTCCACC+AGG + chr7_2:10640224-10640243 None:intergenic 60.0%
! CCTGGTGGACAACCCGGTTA+TGG - chr7_2:10640221-10640240 Msa1048750:CDS 60.0%
CAACCCGGTTATGGGCAACC+AGG - chr7_2:10640230-10640249 Msa1048750:CDS 60.0%
GCACCTGGTTGCCCATAACC+GGG + chr7_2:10640236-10640255 None:intergenic 60.0%
GGCACCTGGTTGCCCATAAC+CGG + chr7_2:10640237-10640256 None:intergenic 60.0%
TTATGGGCAACCAGGTGCCC+AGG - chr7_2:10640238-10640257 Msa1048750:CDS 60.0%
CGCATAACTAGGTGCGGCCT+GGG + chr7_2:10640258-10640277 None:intergenic 60.0%
ACCGGGTTGTCCACCAGGCT+GGG + chr7_2:10640219-10640238 None:intergenic 65.0%
AACCGGGTTGTCCACCAGGC+TGG + chr7_2:10640220-10640239 None:intergenic 65.0%
GCGCATAACTAGGTGCGGCC+TGG + chr7_2:10640259-10640278 None:intergenic 65.0%
! GCCCAGCCTGGTGGACAACC+CGG - chr7_2:10640215-10640234 Msa1048750:CDS 70.0%
CTAGGTGCGGCCTGGGCACC+TGG + chr7_2:10640251-10640270 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 10640080 10640307 10640080 ID=Msa1048750;Name=Msa1048750
chr7_2 mRNA 10640080 10640307 10640080 ID=Msa1048750-mRNA-1;Parent=Msa1048750;Name=Msa1048750-mRNA-1;_AED=0.47;_eAED=0.65;_QI=0|-1|0|1|-1|1|1|0|75
chr7_2 exon 10640080 10640307 10640080 ID=Msa1048750-mRNA-1:exon:2816;Parent=Msa1048750-mRNA-1
chr7_2 CDS 10640080 10640307 10640080 ID=Msa1048750-mRNA-1:cds;Parent=Msa1048750-mRNA-1
Gene Sequence

>Msa1048750

ATGGCAAAAAGCAACAACTATATCTATATGCATCATCGGGGTCATCACAAGCAGCATATCCTACATATGGGTCTGCACTCTGCACTAGCTGCCGATGGTTACAATCACCCGCAGTCTGCACCTGGACAAGTTTATGCCCAGCCTGGTGGACAACCCGGTTATGGGCAACCAGGTGCCCAGGCCGCACCTAGTTATGCGCAAGTTGGTCATACTGGTTATGGATCATAA

Protein sequence

>Msa1048750

MAKSNNYIYMHHRGHHKQHILHMGLHSALAADGYNHPQSAPGQVYAQPGGQPGYGQPGAQAAPSYAQVGHTGYGS*