AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47951


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47951.t1 MTR_8g089610 95.050 202 10 0 1 202 1 202 2.45e-118 334
MS.gene47951.t1 MTR_3g086230 62.927 205 62 10 1 201 1 195 5.64e-64 196
MS.gene47951.t1 MTR_3g086230 73.333 45 11 1 85 129 154 197 3.23e-13 65.9
MS.gene47951.t1 MTR_2g046810 72.848 151 19 4 10 158 1 131 1.76e-53 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47951.t1 AT1G67740 63.057 157 54 2 47 201 32 186 2.96e-49 159
MS.gene47951.t1 AT1G67740 52.308 65 29 2 140 202 51 115 6.00e-12 62.4

Find 57 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTTGTAATGAGCTTATT+TGG 0.218893 4.1:-11052152 None:intergenic
GGTGGTGGGTTAGCAGCTTC+AGG 0.222861 4.1:+11052488 MS.gene47951:CDS
CAAGCTATGGCTGGAATTAA+TGG 0.225855 4.1:-11052380 None:intergenic
AAGCTGCTGGTAGTGATAAT+AGG 0.242204 4.1:+11052561 MS.gene47951:CDS
TGGTAGCACCAGCTATTCTT+TGG 0.258357 4.1:+11052603 MS.gene47951:CDS
AAAGGAGTTATCATTGGTTT+AGG 0.300660 4.1:+11052461 MS.gene47951:CDS
AACCGTATGCGTAACGATAA+AGG 0.307985 4.1:+11052443 MS.gene47951:CDS
GTTATCATTGGTTTAGGTCT+TGG 0.319441 4.1:+11052467 MS.gene47951:CDS
CGTTACGCATACGGTTGATT+TGG 0.341217 4.1:-11052436 None:intergenic
ACTGTCTCATTCTGTTGATT+TGG 0.347582 4.1:-11052658 None:intergenic
AGCTGCTGGTAGTGATAATA+GGG 0.355815 4.1:+11052562 MS.gene47951:CDS
GGTTTGTAATGAGCTTATTT+GGG 0.370406 4.1:-11052151 None:intergenic
CCTGTTGTGGCAATTCCCTT+TGG 0.390126 4.1:-11052206 None:intergenic
GGTAGCACCAGCTATTCTTT+GGG 0.391322 4.1:+11052604 MS.gene47951:CDS
CTCCTTTATCGTTACGCATA+CGG 0.399509 4.1:-11052445 None:intergenic
ATTGGTTTAGGTCTTGGTGG+TGG 0.402929 4.1:+11052473 MS.gene47951:CDS
AACGATAAAGGAGTTATCAT+TGG 0.410305 4.1:+11052455 MS.gene47951:CDS
GTGGTGGGTTAGCAGCTTCA+GGG 0.418077 4.1:+11052489 MS.gene47951:CDS
TTGAGGGAAATGAGGGAAGT+GGG 0.420587 4.1:-11052173 None:intergenic
GGGTCAGCTTCTGTTGTTTG+TGG 0.423859 4.1:+11052583 MS.gene47951:CDS
CTTTGGAAGAAGGTTCTTGA+GGG 0.435326 4.1:-11052189 None:intergenic
CATTAATTCCAGCCATAGCT+TGG 0.438157 4.1:+11052381 MS.gene47951:CDS
CAAGGACTTTGAGTTGATCA+TGG 0.443749 4.1:-11052099 None:intergenic
TGGCAATTCCCTTTGGAAGA+AGG 0.444343 4.1:-11052199 None:intergenic
CTCAAGAACCTTCTTCCAAA+GGG 0.458123 4.1:+11052191 MS.gene47951:CDS
ATCATTGGTTTAGGTCTTGG+TGG 0.458537 4.1:+11052470 MS.gene47951:CDS
TGCTAGTGCTGTTCCTGCTA+TGG 0.458852 4.1:-11052249 None:intergenic
CCAAAGGGAATTGCCACAAC+AGG 0.466016 4.1:+11052206 MS.gene47951:CDS
TTATTTGGGTTGTGGAACTT+TGG 0.470489 4.1:-11052137 None:intergenic
TTGGTTTAGGTCTTGGTGGT+GGG 0.471445 4.1:+11052474 MS.gene47951:CDS
TAATGAGCTTATTTGGGTTG+TGG 0.472958 4.1:-11052145 None:intergenic
ATTAATTCCAGCCATAGCTT+GGG 0.480687 4.1:+11052382 MS.gene47951:CDS
CTGTTGATTTGGTTAAGAGC+AGG 0.484319 4.1:-11052647 None:intergenic
TGGTGAACTAGTAGTGTTCA+AGG 0.485829 4.1:-11052117 None:intergenic
TAGTTTAATGTTTCCTGTTG+TGG 0.486608 4.1:-11052219 None:intergenic
TAAAGAACCCAAAGAATAGC+TGG 0.496379 4.1:-11052611 None:intergenic
CCTCAAGAACCTTCTTCCAA+AGG 0.498567 4.1:+11052190 MS.gene47951:CDS
AAGGTTCTTGAGGGAAATGA+GGG 0.499781 4.1:-11052180 None:intergenic
GATCATGGTCATTGCAATGA+TGG 0.513443 4.1:-11052084 None:intergenic
CTTGAGGGAAATGAGGGAAG+TGG 0.514770 4.1:-11052174 None:intergenic
GCTGCAATTGCTGAAGCTGC+TGG 0.514881 4.1:+11052548 MS.gene47951:CDS
TACAGAACCCAAGCTATGGC+TGG 0.516463 4.1:-11052389 None:intergenic
TGAGGGAAATGAGGGAAGTG+GGG 0.522481 4.1:-11052172 None:intergenic
TGCTGCAAAAGCTGCATCAC+AGG 0.523420 4.1:-11052297 None:intergenic
GAAGGTTCTTGAGGGAAATG+AGG 0.523923 4.1:-11052181 None:intergenic
TCACTAGCCATTGCATCTTG+TGG 0.532256 4.1:-11052521 None:intergenic
AGATGCAATGGCTAGTGAAA+TGG 0.532397 4.1:+11052526 MS.gene47951:CDS
CGGTTGATTTGGTTAAGTGC+TGG 0.533998 4.1:-11052425 None:intergenic
CCTTTGGAAGAAGGTTCTTG+AGG 0.537577 4.1:-11052190 None:intergenic
AAACTATCAACTTCCATAGC+AGG 0.544376 4.1:+11052236 MS.gene47951:CDS
ATAGCAGGAACAGCACTAGC+AGG 0.556768 4.1:+11052251 MS.gene47951:CDS
GTTGTACAGAACCCAAGCTA+TGG 0.571265 4.1:-11052393 None:intergenic
CAAATAGCTGAAATAGCAGA+AGG 0.608735 4.1:+11052323 MS.gene47951:CDS
AGTGTCACCACAAGATGCAA+TGG 0.617349 4.1:+11052514 MS.gene47951:CDS
GCTGCTGGTAGTGATAATAG+GGG 0.720408 4.1:+11052563 MS.gene47951:CDS
GTAACAAAAGTGCTAAACCA+CGG 0.724614 4.1:-11052358 None:intergenic
GAAGGATCATCAGACAACCG+TGG 0.746201 4.1:+11052341 MS.gene47951:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACGATAAAGGAGTTATCAT+TGG + chr4.1:11052455-11052474 MS.gene47951:CDS 30.0%
GGTTTGTAATGAGCTTATTT+GGG - chr4.1:11052154-11052173 None:intergenic 30.0%
TAGTTTAATGTTTCCTGTTG+TGG - chr4.1:11052222-11052241 None:intergenic 30.0%
!! AAAGGAGTTATCATTGGTTT+AGG + chr4.1:11052461-11052480 MS.gene47951:CDS 30.0%
AAACTATCAACTTCCATAGC+AGG + chr4.1:11052236-11052255 MS.gene47951:CDS 35.0%
ATTAATTCCAGCCATAGCTT+GGG + chr4.1:11052382-11052401 MS.gene47951:CDS 35.0%
CAAATAGCTGAAATAGCAGA+AGG + chr4.1:11052323-11052342 MS.gene47951:CDS 35.0%
GGGTTTGTAATGAGCTTATT+TGG - chr4.1:11052155-11052174 None:intergenic 35.0%
TAAAGAACCCAAAGAATAGC+TGG - chr4.1:11052614-11052633 None:intergenic 35.0%
! ACTGTCTCATTCTGTTGATT+TGG - chr4.1:11052661-11052680 None:intergenic 35.0%
!! GTAACAAAAGTGCTAAACCA+CGG - chr4.1:11052361-11052380 None:intergenic 35.0%
!! GTTATCATTGGTTTAGGTCT+TGG + chr4.1:11052467-11052486 MS.gene47951:CDS 35.0%
!! TAATGAGCTTATTTGGGTTG+TGG - chr4.1:11052148-11052167 None:intergenic 35.0%
!! TTATTTGGGTTGTGGAACTT+TGG - chr4.1:11052140-11052159 None:intergenic 35.0%
AACCGTATGCGTAACGATAA+AGG + chr4.1:11052443-11052462 MS.gene47951:CDS 40.0%
AAGGTTCTTGAGGGAAATGA+GGG - chr4.1:11052183-11052202 None:intergenic 40.0%
AGATGCAATGGCTAGTGAAA+TGG + chr4.1:11052526-11052545 MS.gene47951:CDS 40.0%
CAAGCTATGGCTGGAATTAA+TGG - chr4.1:11052383-11052402 None:intergenic 40.0%
CATTAATTCCAGCCATAGCT+TGG + chr4.1:11052381-11052400 MS.gene47951:CDS 40.0%
CTCAAGAACCTTCTTCCAAA+GGG + chr4.1:11052191-11052210 MS.gene47951:CDS 40.0%
CTCCTTTATCGTTACGCATA+CGG - chr4.1:11052448-11052467 None:intergenic 40.0%
GATCATGGTCATTGCAATGA+TGG - chr4.1:11052087-11052106 None:intergenic 40.0%
TGGTGAACTAGTAGTGTTCA+AGG - chr4.1:11052120-11052139 None:intergenic 40.0%
! AAGCTGCTGGTAGTGATAAT+AGG + chr4.1:11052561-11052580 MS.gene47951:CDS 40.0%
! AGCTGCTGGTAGTGATAATA+GGG + chr4.1:11052562-11052581 MS.gene47951:CDS 40.0%
! CTTTGGAAGAAGGTTCTTGA+GGG - chr4.1:11052192-11052211 None:intergenic 40.0%
!! ATCATTGGTTTAGGTCTTGG+TGG + chr4.1:11052470-11052489 MS.gene47951:CDS 40.0%
!! CAAGGACTTTGAGTTGATCA+TGG - chr4.1:11052102-11052121 None:intergenic 40.0%
!! CTGTTGATTTGGTTAAGAGC+AGG - chr4.1:11052650-11052669 None:intergenic 40.0%
AGTGTCACCACAAGATGCAA+TGG + chr4.1:11052514-11052533 MS.gene47951:CDS 45.0%
CCTCAAGAACCTTCTTCCAA+AGG + chr4.1:11052190-11052209 MS.gene47951:CDS 45.0%
CGTTACGCATACGGTTGATT+TGG - chr4.1:11052439-11052458 None:intergenic 45.0%
GAAGGTTCTTGAGGGAAATG+AGG - chr4.1:11052184-11052203 None:intergenic 45.0%
GGTAGCACCAGCTATTCTTT+GGG + chr4.1:11052604-11052623 MS.gene47951:CDS 45.0%
GTTGTACAGAACCCAAGCTA+TGG - chr4.1:11052396-11052415 None:intergenic 45.0%
TCACTAGCCATTGCATCTTG+TGG - chr4.1:11052524-11052543 None:intergenic 45.0%
TGGTAGCACCAGCTATTCTT+TGG + chr4.1:11052603-11052622 MS.gene47951:CDS 45.0%
TTGAGGGAAATGAGGGAAGT+GGG - chr4.1:11052176-11052195 None:intergenic 45.0%
! ATTGGTTTAGGTCTTGGTGG+TGG + chr4.1:11052473-11052492 MS.gene47951:CDS 45.0%
! CCTTTGGAAGAAGGTTCTTG+AGG - chr4.1:11052193-11052212 None:intergenic 45.0%
! GCTGCTGGTAGTGATAATAG+GGG + chr4.1:11052563-11052582 MS.gene47951:CDS 45.0%
! TGGCAATTCCCTTTGGAAGA+AGG - chr4.1:11052202-11052221 None:intergenic 45.0%
! TTGGTTTAGGTCTTGGTGGT+GGG + chr4.1:11052474-11052493 MS.gene47951:CDS 45.0%
!! CGGTTGATTTGGTTAAGTGC+TGG - chr4.1:11052428-11052447 None:intergenic 45.0%
ATAGCAGGAACAGCACTAGC+AGG + chr4.1:11052251-11052270 MS.gene47951:CDS 50.0%
CCAAAGGGAATTGCCACAAC+AGG + chr4.1:11052206-11052225 MS.gene47951:CDS 50.0%
CCTGTTGTGGCAATTCCCTT+TGG - chr4.1:11052209-11052228 None:intergenic 50.0%
CTTGAGGGAAATGAGGGAAG+TGG - chr4.1:11052177-11052196 None:intergenic 50.0%
GAAGGATCATCAGACAACCG+TGG + chr4.1:11052341-11052360 MS.gene47951:CDS 50.0%
TACAGAACCCAAGCTATGGC+TGG - chr4.1:11052392-11052411 None:intergenic 50.0%
TGAGGGAAATGAGGGAAGTG+GGG - chr4.1:11052175-11052194 None:intergenic 50.0%
TGCTGCAAAAGCTGCATCAC+AGG - chr4.1:11052300-11052319 None:intergenic 50.0%
! GGGTCAGCTTCTGTTGTTTG+TGG + chr4.1:11052583-11052602 MS.gene47951:CDS 50.0%
! TGCTAGTGCTGTTCCTGCTA+TGG - chr4.1:11052252-11052271 None:intergenic 50.0%
GCTGCAATTGCTGAAGCTGC+TGG + chr4.1:11052548-11052567 MS.gene47951:CDS 55.0%
!! GTGGTGGGTTAGCAGCTTCA+GGG + chr4.1:11052489-11052508 MS.gene47951:CDS 55.0%
!! GGTGGTGGGTTAGCAGCTTC+AGG + chr4.1:11052488-11052507 MS.gene47951:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 11052074 11052682 11052074 ID=MS.gene47951
chr4.1 mRNA 11052074 11052682 11052074 ID=MS.gene47951.t1;Parent=MS.gene47951
chr4.1 exon 11052074 11052682 11052074 ID=MS.gene47951.t1.exon1;Parent=MS.gene47951.t1
chr4.1 CDS 11052074 11052682 11052074 ID=cds.MS.gene47951.t1;Parent=MS.gene47951.t1
Gene Sequence

>MS.gene47951

ATGGCATCAACCATCATTGCAATGACCATGATCAACTCAAAGTCCTTGAACACTACTAGTTCACCAAAGTTCCACAACCCAAATAAGCTCATTACAAACCCCACTTCCCTCATTTCCCTCAAGAACCTTCTTCCAAAGGGAATTGCCACAACAGGAAACATTAAACTATCAACTTCCATAGCAGGAACAGCACTAGCAGGAGCAATATTCTCAACACTCTCAACCTGTGATGCAGCTTTTGCAGCACAACAAATAGCTGAAATAGCAGAAGGATCATCAGACAACCGTGGTTTAGCACTTTTGTTACCATTAATTCCAGCCATAGCTTGGGTTCTGTACAACATTCTTCAACCAGCACTTAACCAAATCAACCGTATGCGTAACGATAAAGGAGTTATCATTGGTTTAGGTCTTGGTGGTGGGTTAGCAGCTTCAGGGTTAGTGTCACCACAAGATGCAATGGCTAGTGAAATGGCTGCAATTGCTGAAGCTGCTGGTAGTGATAATAGGGGTCAGCTTCTGTTGTTTGTGGTAGCACCAGCTATTCTTTGGGTTCTTTATAATATTTTGCAGCCTGCTCTTAACCAAATCAACAGAATGAGACAGTGA

Protein sequence

>MS.gene47951.t1

MASTIIAMTMINSKSLNTTSSPKFHNPNKLITNPTSLISLKNLLPKGIATTGNIKLSTSIAGTALAGAIFSTLSTCDAAFAAQQIAEIAEGSSDNRGLALLLPLIPAIAWVLYNILQPALNQINRMRNDKGVIIGLGLGGGLAASGLVSPQDAMASEMAAIAEAAGSDNRGQLLLFVVAPAILWVLYNILQPALNQINRMRQ