AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068029


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068029.t1 MTR_4g093560 98.864 176 2 0 1 176 1 176 1.58e-131 365
MS.gene068029.t1 MTR_2g009600 67.630 173 55 1 2 173 3 175 2.94e-86 252
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068029.t1 AT2G14110 66.860 172 56 1 5 175 4 175 2.87e-86 251
MS.gene068029.t1 AT2G14110 68.421 133 42 0 5 137 4 136 4.96e-69 206

Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATAACAGGTTTCAAATT+TGG 0.132575 4.3:+20826809 MS.gene068029:intron
TTTGGGAGTTACAAGTATTT+TGG 0.197480 4.3:+20826827 MS.gene068029:CDS
CAATAACAGGTTTCAAATTT+GGG 0.308384 4.3:+20826810 MS.gene068029:intron
GTTAACAATGGCTTAAATCT+AGG 0.314102 4.3:+20826849 MS.gene068029:CDS
TCGATTTAGATTATACTCTT+TGG 0.338666 4.3:+20826149 MS.gene068029:CDS
CAGAAAATTCATTCAAAGAC+CGG 0.400088 4.3:+20826625 MS.gene068029:CDS
CACCTCAATGTTTGTCGCCC+AGG 0.413707 4.3:+20826435 MS.gene068029:CDS
GAAAGAAGAGATGGAGGAGG+TGG 0.415362 4.3:+20826060 None:intergenic
ATACTCTTTGGCCTTTCTAC+TGG 0.426381 4.3:+20826161 MS.gene068029:CDS
AGAGATGGAGGAGGTGGAGA+AGG 0.429450 4.3:+20826066 None:intergenic
GCTGAGGCAGTGCAAATGAT+AGG 0.438625 4.3:+20826094 MS.gene068029:CDS
CCTCAAAGAACAAGCAGAAA+AGG 0.469406 4.3:+20826916 MS.gene068029:CDS
TGATAACAATAACATGAAAC+CGG 0.472254 4.3:+20826681 MS.gene068029:CDS
GAGTGCACTCAAGATGCCTC+TGG 0.487612 4.3:-20826329 None:intergenic
CAAAGAAGGTGTGTCGTGCT+TGG 0.497441 4.3:-20826296 None:intergenic
TATGTGTGGAGTTGGTGATT+TGG 0.498178 4.3:-20826383 None:intergenic
AGAGAAAGAAGAGATGGAGG+AGG 0.524195 4.3:+20826057 None:intergenic
CTCAAGATGCCTCTGGAATG+TGG 0.524198 4.3:-20826322 None:intergenic
TTGAGTGCACTCAAAGATGA+AGG 0.526991 4.3:+20826340 MS.gene068029:CDS
AAATCGAACACTACGAGCCT+TGG 0.527042 4.3:-20826133 None:intergenic
AATCTAGGAGTATTTAGAGA+AGG 0.528974 4.3:+20826864 MS.gene068029:CDS
GTACAAATTTACCAGTAGAA+AGG 0.530754 4.3:-20826172 None:intergenic
GGAGGAGGTGGAGAAGGTGA+AGG 0.537062 4.3:+20826072 MS.gene068029:CDS
ATCTAGGAGTATTTAGAGAA+GGG 0.541922 4.3:+20826865 MS.gene068029:CDS
GGTGGAGAAGGTGAAGGCTG+AGG 0.552861 4.3:+20826078 MS.gene068029:CDS
CTGGAATGTGGAAACAAAGA+AGG 0.568363 4.3:-20826310 None:intergenic
GTTGTTGCTATGTGTGGAGT+TGG 0.571433 4.3:-20826391 None:intergenic
CATAGAGTTGTAGGGGACAC+CGG 0.577518 4.3:-20826644 None:intergenic
AGTTGGTGATTTGGAAGCAA+TGG 0.603835 4.3:-20826374 None:intergenic
AGATACGTTGTTGCTATGTG+TGG 0.622048 4.3:-20826397 None:intergenic
AACCTGGGCGACAAACATTG+AGG 0.630045 4.3:-20826437 None:intergenic
TCTTTGTTTCCACATTCCAG+AGG 0.646314 4.3:+20826313 MS.gene068029:CDS
AAAAGAGCATAGAGTTGTAG+GGG 0.649290 4.3:-20826651 None:intergenic
AAGGTGTGTCGTGCTTGGAG+CGG 0.652306 4.3:-20826291 None:intergenic
GAATGTTTCAAGTACTACCA+AGG 0.677669 4.3:+20826116 MS.gene068029:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGAGAAATTAAATTT+GGG - chr4.3:20826557-20826576 None:intergenic 10.0%
!! TAAAAAAGAGAAATTAAATT+TGG - chr4.3:20826558-20826577 None:intergenic 10.0%
!!! TTGTATGTATATTTATTTTA+CGG + chr4.3:20826516-20826535 MS.gene068029:intron 10.0%
!! AACAAAATAAAATGATATAC+CGG - chr4.3:20826703-20826722 None:intergenic 15.0%
!!! ATTTCTCTTTTTTATCGTTT+AGG + chr4.3:20826564-20826583 MS.gene068029:intron 20.0%
! AAATAAGCGCAATAAAAAAG+CGG - chr4.3:20826498-20826517 None:intergenic 25.0%
! CAATAACAGGTTTCAAATTT+GGG + chr4.3:20826810-20826829 MS.gene068029:intron 25.0%
! TCGATTTAGATTATACTCTT+TGG + chr4.3:20826149-20826168 MS.gene068029:CDS 25.0%
! TGATAACAATAACATGAAAC+CGG + chr4.3:20826681-20826700 MS.gene068029:CDS 25.0%
!! AAATTCTCTTATTTGTTTGG+AGG + chr4.3:20826743-20826762 MS.gene068029:intron 25.0%
!! CTCAAATTCTCTTATTTGTT+TGG + chr4.3:20826740-20826759 MS.gene068029:intron 25.0%
!! TTTTCATCTTTCCTATTTTC+TGG + chr4.3:20826205-20826224 MS.gene068029:intron 25.0%
!!! ACAAGTATTTTGGTTAACAA+TGG + chr4.3:20826837-20826856 MS.gene068029:CDS 25.0%
CAGAAAATTCATTCAAAGAC+CGG + chr4.3:20826625-20826644 MS.gene068029:CDS 30.0%
CCAATAACAGGTTTCAAATT+TGG + chr4.3:20826809-20826828 MS.gene068029:intron 30.0%
GTACAAATTTACCAGTAGAA+AGG - chr4.3:20826175-20826194 None:intergenic 30.0%
GTTAACAATGGCTTAAATCT+AGG + chr4.3:20826849-20826868 MS.gene068029:CDS 30.0%
! AAAAAGAGCATAGAGTTGTA+GGG - chr4.3:20826655-20826674 None:intergenic 30.0%
! CCAAATTTGAAACCTGTTAT+TGG - chr4.3:20826812-20826831 None:intergenic 30.0%
! TTTGGGAGTTACAAGTATTT+TGG + chr4.3:20826827-20826846 MS.gene068029:CDS 30.0%
!! AATCTAGGAGTATTTAGAGA+AGG + chr4.3:20826864-20826883 MS.gene068029:CDS 30.0%
!! ATCTAGGAGTATTTAGAGAA+GGG + chr4.3:20826865-20826884 MS.gene068029:CDS 30.0%
TAGTGAGTACTCCAGAAAAT+AGG - chr4.3:20826219-20826238 None:intergenic 35.0%
! AAAAGAGCATAGAGTTGTAG+GGG - chr4.3:20826654-20826673 None:intergenic 35.0%
! CACAAGATTTTCGCAAAACT+GGG + chr4.3:20826890-20826909 MS.gene068029:CDS 35.0%
! GAAAAAGAGCATAGAGTTGT+AGG - chr4.3:20826656-20826675 None:intergenic 35.0%
! GAATGTTTCAAGTACTACCA+AGG + chr4.3:20826116-20826135 MS.gene068029:CDS 35.0%
! TCACAAGATTTTCGCAAAAC+TGG + chr4.3:20826889-20826908 MS.gene068029:CDS 35.0%
!! TGATTTGTTGTGCCAATAAC+AGG + chr4.3:20826797-20826816 MS.gene068029:intron 35.0%
ACTGAGGAACAAACAAACCT+GGG - chr4.3:20826455-20826474 None:intergenic 40.0%
AGATACGTTGTTGCTATGTG+TGG - chr4.3:20826400-20826419 None:intergenic 40.0%
CCTCAAAGAACAAGCAGAAA+AGG + chr4.3:20826916-20826935 MS.gene068029:CDS 40.0%
CTGGAATGTGGAAACAAAGA+AGG - chr4.3:20826313-20826332 None:intergenic 40.0%
TTGAGTGCACTCAAAGATGA+AGG + chr4.3:20826340-20826359 MS.gene068029:CDS 40.0%
! ATACTCTTTGGCCTTTCTAC+TGG + chr4.3:20826161-20826180 MS.gene068029:CDS 40.0%
! CCTTTTCTGCTTGTTCTTTG+AGG - chr4.3:20826919-20826938 None:intergenic 40.0%
! TCTTTGTTTCCACATTCCAG+AGG + chr4.3:20826313-20826332 MS.gene068029:CDS 40.0%
!! AGTTGGTGATTTGGAAGCAA+TGG - chr4.3:20826377-20826396 None:intergenic 40.0%
!! TATGTGTGGAGTTGGTGATT+TGG - chr4.3:20826386-20826405 None:intergenic 40.0%
AAATCGAACACTACGAGCCT+TGG - chr4.3:20826136-20826155 None:intergenic 45.0%
AAGTGTTCGTCTTTCCACTG+AGG - chr4.3:20826471-20826490 None:intergenic 45.0%
CACTGAGGAACAAACAAACC+TGG - chr4.3:20826456-20826475 None:intergenic 45.0%
GTTGTTGCTATGTGTGGAGT+TGG - chr4.3:20826394-20826413 None:intergenic 45.0%
! CAGGTTTGTTTGTTCCTCAG+TGG + chr4.3:20826454-20826473 MS.gene068029:intron 45.0%
AACCTGGGCGACAAACATTG+AGG - chr4.3:20826440-20826459 None:intergenic 50.0%
CATAGAGTTGTAGGGGACAC+CGG - chr4.3:20826647-20826666 None:intergenic 50.0%
GCTGAGGCAGTGCAAATGAT+AGG + chr4.3:20826094-20826113 MS.gene068029:CDS 50.0%
! CAAAGAAGGTGTGTCGTGCT+TGG - chr4.3:20826299-20826318 None:intergenic 50.0%
!! CTCAAGATGCCTCTGGAATG+TGG - chr4.3:20826325-20826344 None:intergenic 50.0%
AAGGTGTGTCGTGCTTGGAG+CGG - chr4.3:20826294-20826313 None:intergenic 55.0%
CACCTCAATGTTTGTCGCCC+AGG + chr4.3:20826435-20826454 MS.gene068029:CDS 55.0%
GAGTGCACTCAAGATGCCTC+TGG - chr4.3:20826332-20826351 None:intergenic 55.0%
GGAGGAGGTGGAGAAGGTGA+AGG + chr4.3:20826072-20826091 MS.gene068029:CDS 60.0%
GGTGGAGAAGGTGAAGGCTG+AGG + chr4.3:20826078-20826097 MS.gene068029:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 20826070 20826947 20826070 ID=MS.gene068029
chr4.3 mRNA 20826070 20826947 20826070 ID=MS.gene068029.t1;Parent=MS.gene068029
chr4.3 exon 20826070 20826182 20826070 ID=MS.gene068029.t1.exon1;Parent=MS.gene068029.t1
chr4.3 CDS 20826070 20826182 20826070 ID=cds.MS.gene068029.t1;Parent=MS.gene068029.t1
chr4.3 exon 20826285 20826456 20826285 ID=MS.gene068029.t1.exon2;Parent=MS.gene068029.t1
chr4.3 CDS 20826285 20826456 20826285 ID=cds.MS.gene068029.t1;Parent=MS.gene068029.t1
chr4.3 exon 20826586 20826702 20826586 ID=MS.gene068029.t1.exon3;Parent=MS.gene068029.t1
chr4.3 CDS 20826586 20826702 20826586 ID=cds.MS.gene068029.t1;Parent=MS.gene068029.t1
chr4.3 exon 20826819 20826947 20826819 ID=MS.gene068029.t1.exon4;Parent=MS.gene068029.t1
chr4.3 CDS 20826819 20826947 20826819 ID=cds.MS.gene068029.t1;Parent=MS.gene068029.t1
Gene Sequence

>MS.gene068029

ATGGAGGAGGTGGAGAAGGTGAAGGCTGAGGCAGTGCAAATGATAGGAATGTTTCAAGTACTACCAAGGCTCGTAGTGTTCGATTTAGATTATACTCTTTGGCCTTTCTACTGGTAAATTTGTACATGATACATCTTTTCATCTTTCCTATTTTCTGGAGTACTCACTAAATATTCTTTCTTATTTTACTACACAAAATGAATCTCAAATTTCAGTGAGTGCCGCTCCAAGCACGACACACCTTCTTTGTTTCCACATTCCAGAGGCATCTTGAGTGCACTCAAAGATGAAGGAATTGATGCTGCCATTGCTTCCAAATCACCAACTCCACACATAGCAACAACGTATCTTGACAAACTCAAAATCACCTCAATGTTTGTCGCCCAGGTTTGTTTGTTCCTCAGTGGAAAGACGAACACTTCTATCCGCTTTTTTATTGCGCTTATTTGTATGTATATTTATTTTACGGCACGTTCTTGTTAGACCCAAATTTAATTTCTCTTTTTTATCGTTTAGGAAATATTTTACAGCTCGACACACAAAACAGAACATTTTCAGAAAATTCATTCAAAGACCGGTGTCCCCTACAACTCTATGCTCTTTTTCGACGATGATAACAATAACATGAAACCGGTATATCATTTTATTTTGTTTTTTAATTAATTAAGTTCTCAAATTCTCTTATTTGTTTGGAGGAGTGATTATCAATTTGTCATATCATGTAACATGATTTGTTGTGCCAATAACAGGTTTCAAATTTGGGAGTTACAAGTATTTTGGTTAACAATGGCTTAAATCTAGGAGTATTTAGAGAAGGGCTCACAAGATTTTCGCAAAACTGGGATGCCTCAAAGAACAAGCAGAAAAGGCGCAAGTAA

Protein sequence

>MS.gene068029.t1

MEEVEKVKAEAVQMIGMFQVLPRLVVFDLDYTLWPFYCECRSKHDTPSLFPHSRGILSALKDEGIDAAIASKSPTPHIATTYLDKLKITSMFVAQEIFYSSTHKTEHFQKIHSKTGVPYNSMLFFDDDNNNMKPVSNLGVTSILVNNGLNLGVFREGLTRFSQNWDASKNKQKRRK