AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54467


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54467.t1 MTR_7g007220 67.516 157 6 1 11 122 11 167 7.52e-69 206
MS.gene54467.t1 MTR_7g007200 66.879 157 7 1 11 122 11 167 8.96e-69 205
MS.gene54467.t1 MTR_7g007120 67.308 156 6 1 12 122 12 167 2.65e-68 204
MS.gene54467.t1 MTR_7g007120 66.879 157 7 1 11 122 11 167 2.65e-68 204
MS.gene54467.t1 MTR_7g007120 66.879 157 7 1 11 122 11 167 2.65e-68 204
MS.gene54467.t1 MTR_7g007210 66.883 154 6 1 11 119 11 164 4.36e-66 199
MS.gene54467.t1 MTR_7g007230 65.385 156 9 1 12 122 12 167 5.18e-66 198
MS.gene54467.t1 MTR_6g018310 59.394 165 21 2 4 122 2 166 1.99e-61 187
MS.gene54467.t1 MTR_6g018300 58.182 165 20 3 4 122 2 163 5.20e-58 178
MS.gene54467.t1 MTR_4g127870 50.746 67 33 0 62 128 96 162 4.03e-23 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54467.t1 AT5G38410 50.595 168 33 3 1 122 1 164 5.11e-49 155
MS.gene54467.t1 AT5G38410 49.133 173 33 3 1 122 1 169 3.33e-48 154
MS.gene54467.t1 AT5G38430 49.405 168 35 3 1 122 1 164 2.46e-47 151
MS.gene54467.t1 AT5G38430 49.405 168 35 3 1 122 1 164 2.46e-47 151
MS.gene54467.t1 AT1G67090 49.405 168 35 3 1 122 1 164 2.97e-47 151
MS.gene54467.t1 AT5G38420 49.405 168 35 3 1 122 1 164 4.73e-47 150
MS.gene54467.t1 AT5G38410 51.553 161 35 4 1 122 1 157 8.97e-46 147
MS.gene54467.t1 AT1G67090 43.697 119 47 7 1 118 1 100 4.44e-15 67.8

Find 35 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGTTGACCTTTGTAAC+TGG 0.323260 7.4:+92289151 None:intergenic
GATACTATGACGGACGTTAC+TGG 0.345500 7.4:-92288492 MS.gene54467:CDS
TGATGCTTCTCAAGTGTTGA+AGG 0.410018 7.4:-92288437 MS.gene54467:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.4:-92288466 MS.gene54467:CDS
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.4:-92289227 MS.gene54467:CDS
ATGATACGGACAAAGGCATC+AGG 0.443523 7.4:+92288388 None:intergenic
GGCGCGGTGGCTCCATTCGT+TGG 0.460922 7.4:-92289196 MS.gene54467:CDS
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.4:-92288385 MS.gene54467:CDS
CGTGCCTCTTCGGTACAATC+TGG 0.487772 7.4:-92289217 MS.gene54467:CDS
CTGGTGAACTGTGGTTCTCA+TGG 0.508830 7.4:+92288515 None:intergenic
CTTTGTTGACCTTTGTAACT+GGG 0.514716 7.4:+92289152 None:intergenic
CATGAGAACCACAGTTCACC+AGG 0.519088 7.4:-92288514 MS.gene54467:CDS
TCTTCCACCATTGCTTGCAA+TGG 0.537419 7.4:+92289120 None:intergenic
GTTGGACTAAAGTCCATGGC+TGG 0.555609 7.4:-92289178 MS.gene54467:CDS
TAACGTCCGTCATAGTATCC+TGG 0.560465 7.4:+92288496 None:intergenic
AGCTGAAGAGGACATGATAG+AGG 0.561007 7.4:+92289255 None:intergenic
ACTTCCATTGCAAGCAATGG+TGG 0.573273 7.4:-92289124 MS.gene54467:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.4:+92288374 None:intergenic
ATTACTTCCATTGCAAGCAA+TGG 0.582522 7.4:-92289127 MS.gene54467:CDS
CGCGCCAGATTGTACCGAAG+AGG 0.592678 7.4:+92289213 None:intergenic
GGCTGGCTTCCCAGTTACAA+AGG 0.593536 7.4:-92289161 MS.gene54467:CDS
AACTGTAGTCACAGCTGAAG+AGG 0.597051 7.4:+92289243 None:intergenic
GCATCAGTTGCTCCAAACAA+AGG 0.598394 7.4:+92288454 None:intergenic
TTCGGTACAATCTGGCGCGG+TGG 0.607482 7.4:-92289209 MS.gene54467:CDS
ATTCGTTGGACTAAAGTCCA+TGG 0.609669 7.4:-92289182 MS.gene54467:CDS
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.4:+92289165 None:intergenic
ATGGACTTTAGTCCAACGAA+TGG 0.623442 7.4:+92289184 None:intergenic
CATAGTATCCTGGTGAACTG+TGG 0.641120 7.4:+92288506 None:intergenic
CTCTTCGGTACAATCTGGCG+CGG 0.651184 7.4:-92289212 MS.gene54467:CDS
CAGATTGTACCGAAGAGGCA+CGG 0.652303 7.4:+92289218 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.4:-92289104 MS.gene54467:intron
TGATACGGACAAAGGCATCA+GGG 0.656050 7.4:+92288389 None:intergenic
AGTTCACCAGGATACTATGA+CGG 0.676016 7.4:-92288502 MS.gene54467:CDS
GAATCCGATGATACGGACAA+AGG 0.687909 7.4:+92288381 None:intergenic
ACGGACGTTACTGGACAATG+TGG 0.708236 7.4:-92288483 MS.gene54467:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTCCTTTTCTTTTTAA+AGG - chr7.4:92289200-92289219 MS.gene54467:CDS 15.0%
!!! TTCAAATTTTGTTCAATTTT+AGG - chr7.4:92288623-92288642 MS.gene54467:intron 15.0%
!!! ACATTTCCTTTAAAAAGAAA+AGG + chr7.4:92289209-92289228 None:intergenic 20.0%
!!! TTACATATTTTGATACTAGA+AGG - chr7.4:92288578-92288597 MS.gene54467:intron 20.0%
! AAGAAGTAGAATACCTTATA+AGG - chr7.4:92288721-92288740 MS.gene54467:intron 25.0%
! AGTAAATGAATATTAGACCT+CGG + chr7.4:92288778-92288797 None:intergenic 25.0%
! GAAAAAAAATGTTCCTTCAA+TGG + chr7.4:92289161-92289180 None:intergenic 25.0%
! GAAATTACAAACAGAACAAA+TGG + chr7.4:92289187-92289206 None:intergenic 25.0%
! TTAAAGAGTAACATGTCATA+TGG + chr7.4:92288511-92288530 None:intergenic 25.0%
!!! ATTTTGTTCAATTTTAGGTG+TGG - chr7.4:92288628-92288647 MS.gene54467:intron 25.0%
!!! GATTTTGAAATGCAAATAGA+TGG - chr7.4:92289258-92289277 MS.gene54467:CDS 25.0%
!!! TGTTTGTTTTGATTTCTACT+TGG - chr7.4:92288874-92288893 MS.gene54467:intron 25.0%
AATTTACTATCTGCCATTGA+AGG - chr7.4:92289145-92289164 MS.gene54467:CDS 30.0%
AGTAGAATACCTTATAAGGA+AGG - chr7.4:92288725-92288744 MS.gene54467:intron 30.0%
GGTTACTTGAATAGCATTAA+AGG - chr7.4:92288599-92288618 MS.gene54467:intron 30.0%
GTAGAATACCTTATAAGGAA+GGG - chr7.4:92288726-92288745 MS.gene54467:intron 30.0%
!! ATATAATCGTTGTTTTGTCG+TGG - chr7.4:92288817-92288836 MS.gene54467:intron 30.0%
!!! AAATGTCTCCAGTGTTTTTT+CGG - chr7.4:92289223-92289242 MS.gene54467:CDS 30.0%
AATACCTTATAAGGAAGGGA+TGG - chr7.4:92288730-92288749 MS.gene54467:intron 35.0%
ATACCTTATAAGGAAGGGAT+GGG - chr7.4:92288731-92288750 MS.gene54467:intron 35.0%
ATTACTTCCATTGCAAGCAA+TGG - chr7.4:92288309-92288328 MS.gene54467:intron 35.0%
CAATCCAAACAAGTCATAGA+AGG + chr7.4:92288387-92288406 None:intergenic 35.0%
CATAGAAGGTGATATAAAGC+AGG + chr7.4:92288373-92288392 None:intergenic 35.0%
CTTTGTTGACCTTTGTAACT+GGG + chr7.4:92288287-92288306 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.4:92288657-92288676 None:intergenic 35.0%
TCTCAAATTTAGTAGACTGC+AGG + chr7.4:92289126-92289145 None:intergenic 35.0%
TCTTTGTTGACCTTTGTAAC+TGG + chr7.4:92288288-92288307 None:intergenic 35.0%
!! ATCACCTTCTATGACTTGTT+TGG - chr7.4:92288380-92288399 MS.gene54467:CDS 35.0%
!!! GTTTTTTCGGTCTTTGCTTT+CGG - chr7.4:92289236-92289255 MS.gene54467:CDS 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.4:92288654-92288673 None:intergenic 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.4:92288696-92288715 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.4:92288695-92288714 MS.gene54467:intron 40.0%
AGCAAAGACCGAAAAAACAC+TGG + chr7.4:92289234-92289253 None:intergenic 40.0%
AGTTCACCAGGATACTATGA+CGG - chr7.4:92288934-92288953 MS.gene54467:intron 40.0%
ATGGACTTTAGTCCAACGAA+TGG + chr7.4:92288255-92288274 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.4:92288970-92288989 MS.gene54467:intron 40.0%
ATTCGTTGGACTAAAGTCCA+TGG - chr7.4:92288254-92288273 MS.gene54467:intron 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.4:92288843-92288862 None:intergenic 40.0%
CTACTTGGTTACTTGCAGAA+AGG - chr7.4:92288889-92288908 MS.gene54467:intron 40.0%
TTCTTTCGCCAATTGTTCTC+TGG + chr7.4:92288706-92288725 None:intergenic 40.0%
!! TGATGCTTCTCAAGTGTTGA+AGG - chr7.4:92288999-92289018 MS.gene54467:intron 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.4:92288196-92288215 None:intergenic 45.0%
ACTTCCATTGCAAGCAATGG+TGG - chr7.4:92288312-92288331 MS.gene54467:intron 45.0%
AGCTGAAGAGGACATGATAG+AGG + chr7.4:92288184-92288203 None:intergenic 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.4:92288737-92288756 None:intergenic 45.0%
CATAGTATCCTGGTGAACTG+TGG + chr7.4:92288933-92288952 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.4:92289065-92289084 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.4:92289058-92289077 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.4:92289051-92289070 MS.gene54467:intron 45.0%
GCATCAGTTGCTCCAAACAA+AGG + chr7.4:92288985-92289004 None:intergenic 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.4:92288699-92288718 None:intergenic 45.0%
TAACGTCCGTCATAGTATCC+TGG + chr7.4:92288943-92288962 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.4:92288209-92288228 MS.gene54467:CDS 45.0%
TCTTCCACCATTGCTTGCAA+TGG + chr7.4:92288319-92288338 None:intergenic 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.4:92288332-92288351 MS.gene54467:intron 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.4:92289051-92289070 None:intergenic 45.0%
!! GATACTATGACGGACGTTAC+TGG - chr7.4:92288944-92288963 MS.gene54467:intron 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.4:92289050-92289069 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.4:92288639-92288658 MS.gene54467:intron 45.0%
ACGGACGTTACTGGACAATG+TGG - chr7.4:92288953-92288972 MS.gene54467:intron 50.0%
AGACCTCGGTCTCAAATTCC+AGG + chr7.4:92288764-92288783 None:intergenic 50.0%
CAGATTGTACCGAAGAGGCA+CGG + chr7.4:92288221-92288240 None:intergenic 50.0%
CATGAGAACCACAGTTCACC+AGG - chr7.4:92288922-92288941 MS.gene54467:intron 50.0%
CTGGTGAACTGTGGTTCTCA+TGG + chr7.4:92288924-92288943 None:intergenic 50.0%
GTTGGACTAAAGTCCATGGC+TGG - chr7.4:92288258-92288277 MS.gene54467:intron 50.0%
!! TCGTTGTTTTGTCGTGGCCA+AGG - chr7.4:92288823-92288842 MS.gene54467:intron 50.0%
CGTGCCTCTTCGGTACAATC+TGG - chr7.4:92288219-92288238 MS.gene54467:intron 55.0%
CTCGGTCTCAAATTCCAGGC+AGG + chr7.4:92288760-92288779 None:intergenic 55.0%
CTCTTCGGTACAATCTGGCG+CGG - chr7.4:92288224-92288243 MS.gene54467:intron 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.4:92288274-92288293 None:intergenic 55.0%
! CTGCCTGGAATTTGAGACCG+AGG - chr7.4:92288758-92288777 MS.gene54467:intron 55.0%
!! GGCTGGCTTCCCAGTTACAA+AGG - chr7.4:92288275-92288294 MS.gene54467:intron 55.0%
CGCGCCAGATTGTACCGAAG+AGG + chr7.4:92288226-92288245 None:intergenic 60.0%
TTCGGTACAATCTGGCGCGG+TGG - chr7.4:92288227-92288246 MS.gene54467:intron 60.0%
!! GAAGGGATGGGTTGCCTGCC+TGG - chr7.4:92288743-92288762 MS.gene54467:intron 65.0%
GGCGCGGTGGCTCCATTCGT+TGG - chr7.4:92288240-92288259 MS.gene54467:intron 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 92288174 92289284 92288174 ID=MS.gene54467
chr7.4 mRNA 92288174 92289284 92288174 ID=MS.gene54467.t1;Parent=MS.gene54467
chr7.4 exon 92289105 92289284 92289105 ID=MS.gene54467.t1.exon1;Parent=MS.gene54467.t1
chr7.4 CDS 92289105 92289284 92289105 ID=cds.MS.gene54467.t1;Parent=MS.gene54467.t1
chr7.4 exon 92288366 92288551 92288366 ID=MS.gene54467.t1.exon2;Parent=MS.gene54467.t1
chr7.4 CDS 92288366 92288551 92288366 ID=cds.MS.gene54467.t1;Parent=MS.gene54467.t1
chr7.4 exon 92288174 92288236 92288174 ID=MS.gene54467.t1.exon3;Parent=MS.gene54467.t1
chr7.4 CDS 92288174 92288236 92288174 ID=cds.MS.gene54467.t1;Parent=MS.gene54467.t1
Gene Sequence

>MS.gene54467

ATGGCTTCCTCTATCATGTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCTGGCGCGGTGGCTCCATTCGTTGGACTAAAGTCCATGGCTGGCTTCCCAGTTACAAAGGTCAACAAAGACATTACTTCCATTGCAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAAAGAATATACCTGCTTTATATCACCTTCTATGACTTGTTTGGATTGAGTTATTTTAGTTTATCTAATGACATAAAACTTATGAGACTACTTAAGAGAGTTTATGAAAACAATTTATGACATGCTCATAAAATATTTTAAGCTTATTTCCATATGACATGTTACTCTTTAATGCTTTTGAAATATGTATTTTTTCATTAGATTTAAAAAACGTACATGTTACATATTTTGATACTAGAAGGTTACTTGAATAGCATTAAAGGATTCAAATTTTGTTCAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACTCTTTCATAACTTCCACCATTGACCAGAGAACAATTGGCGAAAGAAGTAGAATACCTTATAAGGAAGGGATGGGTTGCCTGCCTGGAATTTGAGACCGAGGTCTAATATTCATTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGTGGCCAAGGTCACAGTCACAAATTTGTATTTAACATATGTTTGTTTTGATTTCTACTTGGTTACTTGCAGAAAGGATTTGTGTACCATGAGAACCACAGTTCACCAGGATACTATGACGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACTGATGCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAAATTTGAGAATTTACTATCTGCCATTGAAGGAACATTTTTTTTCTCTCCATTTGTTCTGTTTGTAATTTCCTTTTCTTTTTAAAGGAAATGTCTCCAGTGTTTTTTCGGTCTTTGCTTTCGGATTTTGAAATGCAAATAGATGGATAA

Protein sequence

>MS.gene54467.t1

MASSIMSSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKVNKDITSIASNGGRVNCMQKGFVYHENHSSPGYYDGRYWTMWKLPLFGATDASQVLKELDEAIAAYPDAFVRIIGFDNVRQEMSPVFFRSLLSDFEMQIDG