AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061853


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061853.t1 MTR_7g099390 95.683 139 5 1 1 139 1 138 5.46e-93 265
MS.gene061853.t1 MTR_1g069235 57.971 138 47 3 11 138 11 147 8.70e-34 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061853.t1 AT2G20260 52.667 150 48 3 6 139 3 145 4.08e-38 126
MS.gene061853.t1 AT4G28750 66.355 107 25 1 43 138 35 141 5.45e-32 111

Find 29 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCACCTAATAATGTTTC+AGG 0.245319 7.4:+11096853 MS.gene061853:CDS
CCAATTCTAGACTTGTTGTT+AGG 0.258382 7.4:+11096944 MS.gene061853:CDS
CTGTTGCCTGAAACATTATT+AGG 0.270995 7.4:-11096859 None:intergenic
ACACACCTTAGCACCTCTTT+TGG 0.283501 7.4:-11097043 None:intergenic
TTGTTGAATCTTACCACAAC+TGG 0.285947 7.4:-11097626 None:intergenic
ACAACTGGGTAGCGAGTATT+AGG 0.348189 7.4:-11097611 None:intergenic
AACATAGCATCAGCAGCTTC+AGG 0.370983 7.4:+11096823 MS.gene061853:CDS
TCCTACTGGTTCAATGGTAT+TGG 0.371489 7.4:+11097471 MS.gene061853:CDS
TTCTGTTGTTGCTGTTGATC+AGG 0.388075 7.4:+11097494 MS.gene061853:CDS
CCTAACAACAAGTCTAGAAT+TGG 0.406311 7.4:-11096944 None:intergenic
ATCAACAAACAACTATGCTT+TGG 0.412222 7.4:+11097667 MS.gene061853:CDS
TTGGTGTTCTTTGTAGTAGT+AGG 0.427451 7.4:-11096925 None:intergenic
CTATGCTTTGGATGAGATTG+AGG 0.451083 7.4:+11097679 MS.gene061853:CDS
CAATTCTAGACTTGTTGTTA+GGG 0.467358 7.4:+11096945 MS.gene061853:CDS
AAAGAATCCTACTGGTTCAA+TGG 0.482430 7.4:+11097465 MS.gene061853:CDS
TGGTGTTCTTTGTAGTAGTA+GGG 0.490325 7.4:-11096924 None:intergenic
TTCTTAGAAAAGAATCCTAC+TGG 0.502179 7.4:+11097457 MS.gene061853:CDS
GCTGCAACACCAACTGTTGA+AGG 0.512678 7.4:+11096982 MS.gene061853:CDS
CCACCAATTGGTCCCAAAAG+AGG 0.526898 7.4:+11097030 MS.gene061853:CDS
ACATAGCATCAGCAGCTTCA+GGG 0.527887 7.4:+11096824 MS.gene061853:CDS
ACTTGTTGTTAGGGCATCTG+AGG 0.546313 7.4:+11096954 MS.gene061853:CDS
TGGTCCCAAAAGAGGTGCTA+AGG 0.552862 7.4:+11097038 MS.gene061853:CDS
TGTTGTTAGGGCATCTGAGG+AGG 0.552889 7.4:+11096957 MS.gene061853:CDS
GCTGCCTCACCTTCAACAGT+TGG 0.556449 7.4:-11096991 None:intergenic
TAATACTCGCTACCCAGTTG+TGG 0.602018 7.4:+11097613 MS.gene061853:CDS
TGTTGAATCTTACCACAACT+GGG 0.616418 7.4:-11097625 None:intergenic
AACACCAACTGTTGAAGGTG+AGG 0.628187 7.4:+11096987 MS.gene061853:CDS
ATTAGCATCTAGAATGAACA+TGG 0.637216 7.4:+11096894 MS.gene061853:CDS
ACCAATACCATTGAACCAGT+AGG 0.681839 7.4:-11097472 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAAGTATACTTTCATA+CGG - chr7.4:11097108-11097127 None:intergenic 15.0%
!!! CTAAGTTTTATTTATTTTGT+TGG + chr7.4:11097414-11097433 MS.gene061853:intron 15.0%
!!! GTAGTTTTTTTTTAGAAATA+TGG + chr7.4:11097129-11097148 MS.gene061853:intron 15.0%
!!! TTTTTTAGAAATATGGAATT+GGG + chr7.4:11097136-11097155 MS.gene061853:intron 15.0%
!!! TTTTTTTAGAAATATGGAAT+TGG + chr7.4:11097135-11097154 MS.gene061853:intron 15.0%
!! AATTTCCAAATTACAAACTA+GGG - chr7.4:11097200-11097219 None:intergenic 20.0%
!! ATTATAAACTGATAGTAACT+TGG + chr7.4:11097341-11097360 MS.gene061853:intron 20.0%
!! ATTCTTACTAGAAAAAACTA+AGG - chr7.4:11097172-11097191 None:intergenic 20.0%
!! TTCTTACTAGAAAAAACTAA+GGG - chr7.4:11097171-11097190 None:intergenic 20.0%
!!! GTTTTTTCTAGTAAGAATTA+TGG + chr7.4:11097174-11097193 MS.gene061853:intron 20.0%
!!! TGTTTTTCCTACTAAATTAT+TGG - chr7.4:11097382-11097401 None:intergenic 20.0%
! CAATTTCCAAATTACAAACT+AGG - chr7.4:11097201-11097220 None:intergenic 25.0%
! TTCATACGGCATAATTTATT+TGG - chr7.4:11097094-11097113 None:intergenic 25.0%
! TTGTCAACCAATAATTTAGT+AGG + chr7.4:11097372-11097391 MS.gene061853:intron 25.0%
!! CTAGTTTGTAATTTGGAAAT+TGG + chr7.4:11097199-11097218 MS.gene061853:intron 25.0%
ATCAACAAACAACTATGCTT+TGG + chr7.4:11097667-11097686 MS.gene061853:CDS 30.0%
ATCCTACACAACACAATAAA+AGG - chr7.4:11097591-11097610 None:intergenic 30.0%
CTACTTCAAAATCAATCATG+TGG + chr7.4:11097529-11097548 MS.gene061853:intron 30.0%
GAAATATGGAATTGGGAAAA+TGG + chr7.4:11097143-11097162 MS.gene061853:intron 30.0%
TTCTTAGAAAAGAATCCTAC+TGG + chr7.4:11097457-11097476 MS.gene061853:CDS 30.0%
TTGTCACCTAATAATGTTTC+AGG + chr7.4:11096853-11096872 MS.gene061853:CDS 30.0%
! ATTAGCATCTAGAATGAACA+TGG + chr7.4:11096894-11096913 MS.gene061853:CDS 30.0%
! TTCCTTTTATTGTGTTGTGT+AGG + chr7.4:11097586-11097605 MS.gene061853:intron 30.0%
!! CAATTCTAGACTTGTTGTTA+GGG + chr7.4:11096945-11096964 MS.gene061853:CDS 30.0%
!!! CACATGATTGATTTTGAAGT+AGG - chr7.4:11097531-11097550 None:intergenic 30.0%
!!! TTTTGTTGGTTCTACTTAAC+AGG + chr7.4:11097428-11097447 MS.gene061853:intron 30.0%
CTGTTGCCTGAAACATTATT+AGG - chr7.4:11096862-11096881 None:intergenic 35.0%
TGGTGTTCTTTGTAGTAGTA+GGG - chr7.4:11096927-11096946 None:intergenic 35.0%
TGTTGAATCTTACCACAACT+GGG - chr7.4:11097628-11097647 None:intergenic 35.0%
TTGTTGAATCTTACCACAAC+TGG - chr7.4:11097629-11097648 None:intergenic 35.0%
! CCTAACAACAAGTCTAGAAT+TGG - chr7.4:11096947-11096966 None:intergenic 35.0%
! TATGGCCCTAGTTTGTAATT+TGG + chr7.4:11097192-11097211 MS.gene061853:intron 35.0%
! TGGTTCTACTTAACAGGTTA+AGG + chr7.4:11097434-11097453 MS.gene061853:intron 35.0%
! TTGGTGTTCTTTGTAGTAGT+AGG - chr7.4:11096928-11096947 None:intergenic 35.0%
!! AAAGAATCCTACTGGTTCAA+TGG + chr7.4:11097465-11097484 MS.gene061853:CDS 35.0%
!! CCAATTCTAGACTTGTTGTT+AGG + chr7.4:11096944-11096963 MS.gene061853:CDS 35.0%
ACCAATACCATTGAACCAGT+AGG - chr7.4:11097475-11097494 None:intergenic 40.0%
! CTATGCTTTGGATGAGATTG+AGG + chr7.4:11097679-11097698 MS.gene061853:CDS 40.0%
! TTCTGTTGTTGCTGTTGATC+AGG + chr7.4:11097494-11097513 MS.gene061853:CDS 40.0%
!! TCCTACTGGTTCAATGGTAT+TGG + chr7.4:11097471-11097490 MS.gene061853:CDS 40.0%
AAAATCAAGCCCCCACCAAT+TGG + chr7.4:11097018-11097037 MS.gene061853:CDS 45.0%
AACACCAACTGTTGAAGGTG+AGG + chr7.4:11096987-11097006 MS.gene061853:CDS 45.0%
AACATAGCATCAGCAGCTTC+AGG + chr7.4:11096823-11096842 MS.gene061853:CDS 45.0%
ACAACTGGGTAGCGAGTATT+AGG - chr7.4:11097614-11097633 None:intergenic 45.0%
ACATAGCATCAGCAGCTTCA+GGG + chr7.4:11096824-11096843 MS.gene061853:CDS 45.0%
TAATACTCGCTACCCAGTTG+TGG + chr7.4:11097613-11097632 MS.gene061853:CDS 45.0%
! ACACACCTTAGCACCTCTTT+TGG - chr7.4:11097046-11097065 None:intergenic 45.0%
! ATTGGTGGGGGCTTGATTTT+TGG - chr7.4:11097018-11097037 None:intergenic 45.0%
! CACACCTTAGCACCTCTTTT+GGG - chr7.4:11097045-11097064 None:intergenic 45.0%
!! ACTTGTTGTTAGGGCATCTG+AGG + chr7.4:11096954-11096973 MS.gene061853:CDS 45.0%
!!! CTCTTTTGGGACCAATTGGT+GGG - chr7.4:11097032-11097051 None:intergenic 45.0%
!!! TCTTTTGGGACCAATTGGTG+GGG - chr7.4:11097031-11097050 None:intergenic 45.0%
!! AATTATCATATAAATTAAAA+AGG - chr7.4:11097245-11097264 None:intergenic 5.0%
!! ATTATCATATAAATTAAAAA+GGG - chr7.4:11097244-11097263 None:intergenic 5.0%
CCACCAATTGGTCCCAAAAG+AGG + chr7.4:11097030-11097049 MS.gene061853:CDS 50.0%
GCTGCAACACCAACTGTTGA+AGG + chr7.4:11096982-11097001 MS.gene061853:CDS 50.0%
! TGTTGTTAGGGCATCTGAGG+AGG + chr7.4:11096957-11096976 MS.gene061853:CDS 50.0%
!! TGGTCCCAAAAGAGGTGCTA+AGG + chr7.4:11097038-11097057 MS.gene061853:CDS 50.0%
!!! CCTCTTTTGGGACCAATTGG+TGG - chr7.4:11097033-11097052 None:intergenic 50.0%
!!! CTTTTGGGACCAATTGGTGG+GGG - chr7.4:11097030-11097049 None:intergenic 50.0%
!!! GCACCTCTTTTGGGACCAAT+TGG - chr7.4:11097036-11097055 None:intergenic 50.0%
GCTGCCTCACCTTCAACAGT+TGG - chr7.4:11096994-11097013 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 11096811 11097712 11096811 ID=MS.gene061853
chr7.4 mRNA 11096811 11097712 11096811 ID=MS.gene061853.t1;Parent=MS.gene061853
chr7.4 exon 11096811 11097059 11096811 ID=MS.gene061853.t1.exon1;Parent=MS.gene061853.t1
chr7.4 CDS 11096811 11097059 11096811 ID=cds.MS.gene061853.t1;Parent=MS.gene061853.t1
chr7.4 exon 11097450 11097515 11097450 ID=MS.gene061853.t1.exon2;Parent=MS.gene061853.t1
chr7.4 CDS 11097450 11097515 11097450 ID=cds.MS.gene061853.t1;Parent=MS.gene061853.t1
chr7.4 exon 11097608 11097712 11097608 ID=MS.gene061853.t1.exon3;Parent=MS.gene061853.t1
chr7.4 CDS 11097608 11097712 11097608 ID=cds.MS.gene061853.t1;Parent=MS.gene061853.t1
Gene Sequence

>MS.gene061853

ATGGCTAGTTGCAACATAGCATCAGCAGCTTCAGGGTTTGTGTTGTCACCTAATAATGTTTCAGGCAACAGAAACACTAATTTATTAGCATCTAGAATGAACATGGTGATGTACCCTACTACTACAAAGAACACCAATTCTAGACTTGTTGTTAGGGCATCTGAGGAGGCTGCTGCAACACCAACTGTTGAAGGTGAGGCAGCTCCAAAAATCAAGCCCCCACCAATTGGTCCCAAAAGAGGTGCTAAGGTGTGTTATATTTTGTGTCATCTATACACTTCCAAATAAATTATGCCGTATGAAAGTATACTTTAATTTGTAGTTTTTTTTTAGAAATATGGAATTGGGAAAATGGTTCCCTTAGTTTTTTCTAGTAAGAATTATGGCCCTAGTTTGTAATTTGGAAATTGGAATCACATTTTATGTCACACCCTTTTTAATTTATATGATAATTGTAAGTTTAAGTATGCAAAAATATTTTTATACAAATAAGTATAGCACTAAAATCATGCAATTGTTTTGAAACTTCGATTATAAACTGATAGTAACTTGGAATTTGTGTTGTCAACCAATAATTTAGTAGGAAAAACATTTTTTCTTCTTCTAAGTTTTATTTATTTTGTTGGTTCTACTTAACAGGTTAAGGTTCTTAGAAAAGAATCCTACTGGTTCAATGGTATTGGTTCTGTTGTTGCTGTTGATCAGGTTAGTTTCTCTCCTACTTCAAAATCAATCATGTGGATTTTGATCTCTTTGTAAGTTTCACTATATTAATTTCCTTTTATTGTGTTGTGTAGGATCCTAATACTCGCTACCCAGTTGTGGTAAGATTCAACAAAGTTAACTATGCAAATGTATCAACAAACAACTATGCTTTGGATGAGATTGAGGAAGTCAAATGA

Protein sequence

>MS.gene061853.t1

MASCNIASAASGFVLSPNNVSGNRNTNLLASRMNMVMYPTTTKNTNSRLVVRASEEAAATPTVEGEAAPKIKPPPIGPKRGAKVKVLRKESYWFNGIGSVVAVDQDPNTRYPVVVRFNKVNYANVSTNNYALDEIEEVK