AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47833


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47833.t1 MTR_4g015570 99.167 120 1 0 1 120 170 289 6.57e-83 244
MS.gene47833.t1 MTR_3g070340 82.524 103 18 0 1 103 173 275 2.66e-54 171
MS.gene47833.t1 MTR_6g033320 49.541 109 52 3 7 113 127 234 2.97e-29 106
MS.gene47833.t1 MTR_6g060175 43.802 121 64 3 3 120 153 272 5.00e-25 95.9
MS.gene47833.t1 MTR_6g060175 43.802 121 64 3 3 120 163 282 6.57e-25 95.9
MS.gene47833.t1 MTR_2g081090 44.545 110 55 3 6 109 113 222 1.02e-21 86.3
MS.gene47833.t1 MTR_6g012110 45.133 113 55 4 6 112 142 253 1.80e-21 86.3
MS.gene47833.t1 MTR_6g012080 45.133 113 55 4 6 112 142 253 1.94e-21 86.3
MS.gene47833.t1 MTR_6g011880 45.133 113 55 4 6 112 142 253 2.34e-21 85.9
MS.gene47833.t1 MTR_2g081090 44.545 110 55 3 6 109 140 249 2.35e-21 85.9
MS.gene47833.t1 MTR_6g011890 45.133 113 55 4 6 112 142 253 2.39e-21 85.9
MS.gene47833.t1 MTR_6g011870 45.133 113 55 4 6 112 142 253 2.42e-21 85.9
MS.gene47833.t1 MTR_6g043600 46.018 113 54 4 6 112 106 217 3.53e-21 84.7
MS.gene47833.t1 MTR_4g094605 46.018 113 54 4 6 112 143 254 4.46e-21 85.1
MS.gene47833.t1 MTR_1g026550 52.055 73 35 0 47 119 257 329 1.31e-20 85.1
MS.gene47833.t1 MTR_3g101670 40.000 120 53 1 20 120 146 265 3.15e-20 83.2
MS.gene47833.t1 MTR_5g097280 43.363 113 51 5 6 109 142 250 7.89e-19 79.3
MS.gene47833.t1 MTR_2g042720 37.815 119 56 3 19 119 148 266 8.36e-19 79.3
MS.gene47833.t1 MTR_3g435460 38.843 121 52 3 17 120 76 191 8.73e-18 75.1
MS.gene47833.t1 MTR_8g023790 38.983 118 54 2 19 118 149 266 1.37e-17 75.9
MS.gene47833.t1 MTR_3g435460 38.843 121 52 3 17 120 137 252 2.68e-17 75.1
MS.gene47833.t1 MTR_5g098770 44.444 72 40 0 47 118 21 92 4.54e-17 70.9
MS.gene47833.t1 MTR_5g098785 36.207 116 55 2 20 116 158 273 2.05e-16 73.2
MS.gene47833.t1 MTR_5g098780 36.207 116 55 2 20 116 156 271 2.68e-16 72.8
MS.gene47833.t1 MTR_2g436960 50.000 72 31 2 47 118 33 99 3.92e-14 63.5
MS.gene47833.t1 MTR_2g008610 37.607 117 40 4 15 99 142 257 2.16e-12 62.0
MS.gene47833.t1 MTR_0003s0060 44.118 68 34 1 53 120 31 94 2.23e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47833.t1 AT3G08940 94.167 120 7 0 1 120 168 287 6.87e-79 234
MS.gene47833.t1 AT5G01530 94.167 120 7 0 1 120 171 290 1.09e-78 233
MS.gene47833.t1 AT2G40100 80.583 103 19 1 1 103 172 273 2.84e-52 166
MS.gene47833.t1 AT3G54890 52.252 111 50 3 7 115 126 235 2.97e-32 114
MS.gene47833.t1 AT4G10340 44.628 121 63 3 3 120 154 273 2.38e-24 94.4
MS.gene47833.t1 AT1G29920 47.368 114 52 5 6 112 143 255 4.99e-23 90.5
MS.gene47833.t1 AT1G29910 47.368 114 52 5 6 112 143 255 4.99e-23 90.5
MS.gene47833.t1 AT1G29930 47.368 114 52 5 6 112 143 255 4.99e-23 90.5
MS.gene47833.t1 AT2G34430 47.368 114 52 4 6 112 142 254 6.46e-23 90.1
MS.gene47833.t1 AT2G34420 47.368 114 52 4 6 112 141 253 6.91e-23 90.1
MS.gene47833.t1 AT5G54270 46.364 110 53 2 6 109 141 250 1.22e-22 89.4
MS.gene47833.t1 AT1G76570 58.824 68 28 0 47 114 255 322 5.30e-21 85.9
MS.gene47833.t1 AT1G45474 39.516 124 55 1 17 120 131 254 9.36e-21 84.3
MS.gene47833.t1 AT1G45474 39.516 124 55 1 17 120 131 254 9.36e-21 84.3
MS.gene47833.t1 AT2G05070 44.248 113 50 5 6 109 142 250 4.66e-19 80.1
MS.gene47833.t1 AT2G05100 44.248 113 50 5 6 109 142 250 4.85e-19 80.1
MS.gene47833.t1 AT3G27690 43.363 113 51 5 6 109 175 283 2.05e-18 78.6
MS.gene47833.t1 AT3G27690 43.363 113 51 5 6 109 143 251 2.53e-18 78.2
MS.gene47833.t1 AT3G61470 38.983 118 54 2 19 118 138 255 3.12e-17 75.1
MS.gene47833.t1 AT3G47470 38.655 119 51 3 17 118 138 251 3.26e-17 74.7
MS.gene47833.t1 AT1G61520 35.345 116 56 2 20 116 99 214 5.81e-17 73.6
MS.gene47833.t1 AT1G61520 35.345 116 56 2 20 116 154 269 1.18e-16 73.6
MS.gene47833.t1 AT1G61520 35.345 116 56 2 20 116 154 269 1.18e-16 73.6
MS.gene47833.t1 AT5G28450 45.783 83 44 1 37 118 89 171 1.04e-15 69.3
MS.gene47833.t1 AT3G54890 47.222 72 36 2 7 77 126 196 1.31e-15 70.1
MS.gene47833.t1 AT1G19150 36.134 119 58 3 19 119 151 269 2.86e-15 70.1
MS.gene47833.t1 AT1G15820 37.607 117 40 4 15 99 139 254 1.58e-12 62.4
MS.gene47833.t1 AT2G05100 42.000 100 45 5 6 96 142 237 4.40e-12 61.6

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATCGAAGTTCTAGTAAT+TGG 0.298063 4.4:+84920791 MS.gene47833:CDS
TCTCTATCACAACACTTATC+TGG 0.311866 4.4:+84920771 MS.gene47833:CDS
CTTGCTATGGTTGCTTTCCT+TGG 0.358398 4.4:+84920956 MS.gene47833:CDS
GTAAAGGACCACTTAACAAT+TGG 0.364076 4.4:+84921005 MS.gene47833:CDS
TTTCAGGTTGAGCTAGTAGA+AGG 0.373969 4.4:+84920719 None:intergenic
AAAGAAAGCAACACTTCAAT+TGG 0.404848 4.4:+84920913 MS.gene47833:CDS
CATCAAGCTCAGCATTTCTC+TGG 0.414677 4.4:-84920826 None:intergenic
GCTGTTCAAGCTGCTGCTAC+TGG 0.427306 4.4:+84920983 MS.gene47833:CDS
GTAGAAGGATCAACATATCT+TGG 0.431470 4.4:+84920734 MS.gene47833:CDS
CAAGCTGCTGCTACTGGTAA+AGG 0.448841 4.4:+84920989 MS.gene47833:CDS
GGAAAATTCTTTGACCCACT+TGG 0.458494 4.4:+84920872 MS.gene47833:CDS
ATAAGTGTTGTGATAGAGAA+TGG 0.479242 4.4:-84920767 None:intergenic
GGAAGAAAAGGTATCAATGA+TGG 0.486648 4.4:-84921059 None:intergenic
CAAGAACTAAGAGGAAGAAA+AGG 0.500205 4.4:-84921071 None:intergenic
TAAAGGACCACTTAACAATT+GGG 0.525889 4.4:+84921006 MS.gene47833:CDS
TGAGTAGCCCAATTGTTAAG+TGG 0.534802 4.4:-84921013 None:intergenic
TGAGTTAATCAAGAACTAAG+AGG 0.538848 4.4:-84921080 None:intergenic
CAAACATGCTCGTCTTGCTA+TGG 0.544135 4.4:+84920943 MS.gene47833:CDS
GTTGTGATAGAGAATGGAAG+TGG 0.611749 4.4:-84920761 None:intergenic
TCAATGATGGTAGTGTGAAG+AGG 0.631393 4.4:-84921046 None:intergenic
GGTCAGATGCTAAGCCAAGT+GGG 0.631916 4.4:-84920886 None:intergenic
GGGTCAGATGCTAAGCCAAG+TGG 0.635931 4.4:-84920887 None:intergenic
CAGCTTGAACAGCAAAACCA+AGG 0.638361 4.4:-84920973 None:intergenic
GAAAAGAGACTATACCCAGG+TGG 0.723166 4.4:+84920851 MS.gene47833:CDS
GCAGAAAAGAGACTATACCC+AGG 0.746677 4.4:+84920848 MS.gene47833:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGAAAGCAACACTTCAAT+TGG + chr4.4:84920913-84920932 MS.gene47833:CDS 30.0%
TAAAGGACCACTTAACAATT+GGG + chr4.4:84921006-84921025 MS.gene47833:CDS 30.0%
! ATAAGTGTTGTGATAGAGAA+TGG - chr4.4:84920770-84920789 None:intergenic 30.0%
GTAAAGGACCACTTAACAAT+TGG + chr4.4:84921005-84921024 MS.gene47833:CDS 35.0%
GTAGAAGGATCAACATATCT+TGG + chr4.4:84920734-84920753 MS.gene47833:CDS 35.0%
TCTCTATCACAACACTTATC+TGG + chr4.4:84920771-84920790 MS.gene47833:CDS 35.0%
! TGATACCTTTTCTTCCTCTT+AGG + chr4.4:84921066-84921085 MS.gene47833:CDS 35.0%
! TGGATCGAAGTTCTAGTAAT+TGG + chr4.4:84920791-84920810 MS.gene47833:CDS 35.0%
!! GGAAGAAAAGGTATCAATGA+TGG - chr4.4:84921062-84921081 None:intergenic 35.0%
!!! GAAGTGTTGCTTTCTTTTCT+GGG - chr4.4:84920910-84920929 None:intergenic 35.0%
!!! TGAAGTGTTGCTTTCTTTTC+TGG - chr4.4:84920911-84920930 None:intergenic 35.0%
GGAAAATTCTTTGACCCACT+TGG + chr4.4:84920872-84920891 MS.gene47833:CDS 40.0%
GTTGTGATAGAGAATGGAAG+TGG - chr4.4:84920764-84920783 None:intergenic 40.0%
TGAGTAGCCCAATTGTTAAG+TGG - chr4.4:84921016-84921035 None:intergenic 40.0%
! GGTCAAAGAATTTTCCACCT+GGG - chr4.4:84920868-84920887 None:intergenic 40.0%
! TCAATGATGGTAGTGTGAAG+AGG - chr4.4:84921049-84921068 None:intergenic 40.0%
CAAACATGCTCGTCTTGCTA+TGG + chr4.4:84920943-84920962 MS.gene47833:CDS 45.0%
CAGCTTGAACAGCAAAACCA+AGG - chr4.4:84920976-84920995 None:intergenic 45.0%
CATCAAGCTCAGCATTTCTC+TGG - chr4.4:84920829-84920848 None:intergenic 45.0%
GAAAAGAGACTATACCCAGG+TGG + chr4.4:84920851-84920870 MS.gene47833:CDS 45.0%
GCAGAAAAGAGACTATACCC+AGG + chr4.4:84920848-84920867 MS.gene47833:CDS 45.0%
! CTTGCTATGGTTGCTTTCCT+TGG + chr4.4:84920956-84920975 MS.gene47833:CDS 45.0%
! GGGTCAAAGAATTTTCCACC+TGG - chr4.4:84920869-84920888 None:intergenic 45.0%
CAAGCTGCTGCTACTGGTAA+AGG + chr4.4:84920989-84921008 MS.gene47833:CDS 50.0%
GGTCAGATGCTAAGCCAAGT+GGG - chr4.4:84920889-84920908 None:intergenic 50.0%
GCTGTTCAAGCTGCTGCTAC+TGG + chr4.4:84920983-84921002 MS.gene47833:CDS 55.0%
GGGTCAGATGCTAAGCCAAG+TGG - chr4.4:84920890-84920909 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 84920725 84921087 84920725 ID=MS.gene47833
chr4.4 mRNA 84920725 84921087 84920725 ID=MS.gene47833.t1;Parent=MS.gene47833
chr4.4 exon 84920725 84921087 84920725 ID=MS.gene47833.t1.exon1;Parent=MS.gene47833.t1
chr4.4 CDS 84920725 84921087 84920725 ID=cds.MS.gene47833.t1;Parent=MS.gene47833.t1
Gene Sequence

>MS.gene47833

GTTGAGCTAGTAGAAGGATCAACATATCTTGGACAACCACTTCCATTCTCTATCACAACACTTATCTGGATCGAAGTTCTAGTAATTGGCTACATTGAATTCCAGAGAAATGCTGAGCTTGATGCAGAAAAGAGACTATACCCAGGTGGAAAATTCTTTGACCCACTTGGCTTAGCATCTGACCCAGAAAAGAAAGCAACACTTCAATTGGCAGAAATCAAACATGCTCGTCTTGCTATGGTTGCTTTCCTTGGTTTTGCTGTTCAAGCTGCTGCTACTGGTAAAGGACCACTTAACAATTGGGCTACTCATTTGAGTGATCCTCTTCACACTACCATCATTGATACCTTTTCTTCCTCTTAG

Protein sequence

>MS.gene47833.t1

VELVEGSTYLGQPLPFSITTLIWIEVLVIGYIEFQRNAELDAEKRLYPGGKFFDPLGLASDPEKKATLQLAEIKHARLAMVAFLGFAVQAAATGKGPLNNWATHLSDPLHTTIIDTFSSS