AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0002460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0002460 MtrunA17_Chr4g0056091 94.062 320 14 3 1 320 1 315 0.0 555
Msa0002460 MtrunA17_Chr7g0231181 42.507 367 152 13 1 320 1 355 3.18e-67 214
Msa0002460 MtrunA17_Chr5g0399261 42.415 323 104 14 1 305 5 263 1.24e-57 187
Msa0002460 MtrunA17_Chr4g0012691 35.326 368 155 10 1 308 2 346 1.40e-42 150
Msa0002460 MtrunA17_Chr4g0018761 27.597 308 155 13 6 307 9 254 2.25e-18 83.2
Msa0002460 MtrunA17_Chr1g0152661 28.426 197 102 6 132 306 14 193 1.41e-11 63.5
Msa0002460 MtrunA17_Chr4g0061301 29.630 216 84 8 3 176 10 199 1.57e-11 65.1
Msa0002460 MtrunA17_Chr4g0061301 50.000 62 27 1 252 313 436 493 2.36e-11 64.3
Msa0002460 MtrunA17_Chr4g0043071 50.000 60 28 1 251 310 441 498 5.36e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0002460 AT3G60580.1 36.747 332 149 11 1 317 1 286 2.09e-47 161
Msa0002460 AT1G02030.1 36.145 332 128 11 2 317 3 266 1.28e-41 145
Msa0002460 AT2G45120.1 35.398 339 172 10 1 319 1 312 1.24e-39 142
Msa0002460 AT4G16610.1 38.372 172 85 4 3 173 4 155 1.20e-24 99.4
Msa0002460 AT1G26610.1 46.575 73 36 1 238 310 386 455 7.67e-13 69.3
Msa0002460 AT5G61470.1 43.902 82 36 3 205 276 218 299 3.37e-11 63.5

Find 48 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTGTTGTAGAAAATTC+TGG 0.230051 1_1:-4357800 None:intergenic
CCAAACACCTTAAAACAAAA+TGG 0.311153 1_1:-4358191 None:intergenic
TCACGTACAAAAGAAAATTC+AGG 0.344635 1_1:-4357660 None:intergenic
CACATAGCAACATCTTCTTC+AGG 0.374474 1_1:-4357906 None:intergenic
GGCACCATGAAACTCGGCTT+TGG 0.378686 1_1:-4357825 None:intergenic
TCTTTGAATCTAGCAGCAAT+AGG 0.389886 1_1:-4358299 None:intergenic
GAAACCAAAGCCGAGTTTCA+TGG 0.392923 1_1:+4357821 Msa0002460:CDS
AAGCTTTGTTCAAGGACGTT+TGG 0.415966 1_1:+4357450 Msa0002460:CDS
TCCGATGATGAGAAAGAATC+AGG 0.418255 1_1:-4357549 None:intergenic
TAGATCGTTGTTGACGAGTC+GGG 0.431866 1_1:-4357737 None:intergenic
TGATGCTTTCGCGAGACAAA+TGG 0.443203 1_1:+4357934 Msa0002460:CDS
ACCTGATTCTTTCTCATCAT+CGG 0.450240 1_1:+4357548 Msa0002460:CDS
TTGTTGTCGTCGTTGTTGTT+TGG 0.451043 1_1:-4357774 None:intergenic
GTGAATTGTGATGGAGTTGT+TGG 0.452696 1_1:-4357846 None:intergenic
GTTTCGATCTTCAAGAGCAT+TGG 0.459910 1_1:+4358088 Msa0002460:CDS
TTTCGATCTTCAAGAGCATT+GGG 0.469548 1_1:+4358089 Msa0002460:CDS
TTTGGTTCTGGTCAAGCACT+TGG 0.469573 1_1:+4358209 Msa0002460:CDS
CTCGTCAACAACGATCTAAA+CGG 0.483916 1_1:+4357742 Msa0002460:CDS
GGTTCTGGTCAAGCACTTGG+TGG 0.484093 1_1:+4358212 Msa0002460:CDS
TGAAGAAGAAGAGGGATCGG+TGG 0.488736 1_1:+4357995 Msa0002460:CDS
TCATCTTCTTCAAGTGGAGC+TGG 0.500458 1_1:-4358344 None:intergenic
ACAAATGCAAGCTTTGTTCA+AGG 0.502681 1_1:+4357442 Msa0002460:CDS
GCGAAGAATGGTAATGGTAA+TGG 0.505680 1_1:+4358143 Msa0002460:CDS
CAGTGGAAGTTGATGAAGAA+GGG 0.513438 1_1:-4358259 None:intergenic
ACATAGCAACATCTTCTTCA+GGG 0.525812 1_1:-4357905 None:intergenic
AAGTTATTGAAACGAGTTCG+TGG 0.531632 1_1:+4358035 Msa0002460:CDS
TGTCGCGATGAAGCGAAGAA+TGG 0.548591 1_1:+4358131 Msa0002460:CDS
TCTTGTTCAGTGAATTGTGA+TGG 0.549593 1_1:-4357855 None:intergenic
TTTGGCAATGGAAGAGCACT+TGG 0.550999 1_1:+4357468 Msa0002460:CDS
GTTGTTGGCACCATGAAACT+CGG 0.554243 1_1:-4357831 None:intergenic
TTAGATCGTTGTTGACGAGT+CGG 0.558622 1_1:-4357738 None:intergenic
GGAACAAGATGAAGAAGAAG+AGG 0.558902 1_1:+4357986 Msa0002460:CDS
AGATCATCATCTTCTTCAAG+TGG 0.559232 1_1:-4358350 None:intergenic
ACAGTGGAAGTTGATGAAGA+AGG 0.561357 1_1:-4358260 None:intergenic
GGCAATGGAAGAGCACTTGG+TGG 0.563472 1_1:+4357471 Msa0002460:CDS
TGTTCAAGGACGTTTGGCAA+TGG 0.572618 1_1:+4357456 Msa0002460:CDS
GATGAAGCGAAGAATGGTAA+TGG 0.574456 1_1:+4358137 Msa0002460:CDS
AGCACTTGGTGGACACATGA+AGG 0.581864 1_1:+4357482 Msa0002460:CDS
AGATGAAGAAGAAGAGGGAT+CGG 0.594748 1_1:+4357992 Msa0002460:CDS
AGTTATTGAAACGAGTTCGT+GGG 0.596995 1_1:+4358036 Msa0002460:CDS
GTATCAGAAACTGAACTCAC+AGG 0.612015 1_1:-4357879 None:intergenic
CATAGCAACATCTTCTTCAG+GGG 0.618873 1_1:-4357904 None:intergenic
ATTAACATTAACGTTAACAG+TGG 0.623956 1_1:-4358276 None:intergenic
GAACAAGATGAAGAAGAAGA+GGG 0.633530 1_1:+4357987 Msa0002460:CDS
AGCATTTGTGTGAAAACTGT+GGG 0.639279 1_1:+4358060 Msa0002460:CDS
ATCGGTGGAGAAAATATCGA+AGG 0.639870 1_1:+4358010 Msa0002460:CDS
GATGAACAACATCAACAATG+TGG 0.658258 1_1:+4357965 Msa0002460:CDS
AAGCATTTGTGTGAAAACTG+TGG 0.714401 1_1:+4358059 Msa0002460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATGTCCATTTTGTTTTA+AGG + chr1_1:4358184-4358203 Msa0002460:CDS 25.0%
! ATTAACATTAACGTTAACAG+TGG - chr1_1:4358279-4358298 None:intergenic 25.0%
!!! TGAATGTCCATTTTGTTTTA+AGG + chr1_1:4358184-4358203 Msa0002460:CDS 25.0%
! ATTAACATTAACGTTAACAG+TGG - chr1_1:4358279-4358298 None:intergenic 25.0%
TCACGTACAAAAGAAAATTC+AGG - chr1_1:4357663-4357682 None:intergenic 30.0%
TCTTCTGTTGTAGAAAATTC+TGG - chr1_1:4357803-4357822 None:intergenic 30.0%
CCAAACACCTTAAAACAAAA+TGG - chr1_1:4358194-4358213 None:intergenic 30.0%
!!! CCATTTTGTTTTAAGGTGTT+TGG + chr1_1:4358191-4358210 Msa0002460:CDS 30.0%
TCACGTACAAAAGAAAATTC+AGG - chr1_1:4357663-4357682 None:intergenic 30.0%
TCTTCTGTTGTAGAAAATTC+TGG - chr1_1:4357803-4357822 None:intergenic 30.0%
CCAAACACCTTAAAACAAAA+TGG - chr1_1:4358194-4358213 None:intergenic 30.0%
!!! CCATTTTGTTTTAAGGTGTT+TGG + chr1_1:4358191-4358210 Msa0002460:CDS 30.0%
!! ACAAATGCAAGCTTTGTTCA+AGG + chr1_1:4357442-4357461 Msa0002460:CDS 35.0%
ACCTGATTCTTTCTCATCAT+CGG + chr1_1:4357548-4357567 Msa0002460:CDS 35.0%
!!! GCAACTTTGAGACTTTTCTT+GGG - chr1_1:4357636-4357655 None:intergenic 35.0%
!!! TGCAACTTTGAGACTTTTCT+TGG - chr1_1:4357637-4357656 None:intergenic 35.0%
TCTTGTTCAGTGAATTGTGA+TGG - chr1_1:4357858-4357877 None:intergenic 35.0%
ACATAGCAACATCTTCTTCA+GGG - chr1_1:4357908-4357927 None:intergenic 35.0%
GATGAACAACATCAACAATG+TGG + chr1_1:4357965-4357984 Msa0002460:CDS 35.0%
GAACAAGATGAAGAAGAAGA+GGG + chr1_1:4357987-4358006 Msa0002460:CDS 35.0%
AAGTTATTGAAACGAGTTCG+TGG + chr1_1:4358035-4358054 Msa0002460:CDS 35.0%
AGTTATTGAAACGAGTTCGT+GGG + chr1_1:4358036-4358055 Msa0002460:CDS 35.0%
AAGCATTTGTGTGAAAACTG+TGG + chr1_1:4358059-4358078 Msa0002460:CDS 35.0%
AGCATTTGTGTGAAAACTGT+GGG + chr1_1:4358060-4358079 Msa0002460:CDS 35.0%
!! TTTCGATCTTCAAGAGCATT+GGG + chr1_1:4358089-4358108 Msa0002460:CDS 35.0%
!!! TGTTTTAAGGTGTTTGGTTC+TGG + chr1_1:4358197-4358216 Msa0002460:CDS 35.0%
TCTTTGAATCTAGCAGCAAT+AGG - chr1_1:4358302-4358321 None:intergenic 35.0%
AGATCATCATCTTCTTCAAG+TGG - chr1_1:4358353-4358372 None:intergenic 35.0%
!! ACAAATGCAAGCTTTGTTCA+AGG + chr1_1:4357442-4357461 Msa0002460:CDS 35.0%
ACCTGATTCTTTCTCATCAT+CGG + chr1_1:4357548-4357567 Msa0002460:CDS 35.0%
!!! GCAACTTTGAGACTTTTCTT+GGG - chr1_1:4357636-4357655 None:intergenic 35.0%
!!! TGCAACTTTGAGACTTTTCT+TGG - chr1_1:4357637-4357656 None:intergenic 35.0%
TCTTGTTCAGTGAATTGTGA+TGG - chr1_1:4357858-4357877 None:intergenic 35.0%
ACATAGCAACATCTTCTTCA+GGG - chr1_1:4357908-4357927 None:intergenic 35.0%
GATGAACAACATCAACAATG+TGG + chr1_1:4357965-4357984 Msa0002460:CDS 35.0%
GAACAAGATGAAGAAGAAGA+GGG + chr1_1:4357987-4358006 Msa0002460:CDS 35.0%
AAGTTATTGAAACGAGTTCG+TGG + chr1_1:4358035-4358054 Msa0002460:CDS 35.0%
AGTTATTGAAACGAGTTCGT+GGG + chr1_1:4358036-4358055 Msa0002460:CDS 35.0%
AAGCATTTGTGTGAAAACTG+TGG + chr1_1:4358059-4358078 Msa0002460:CDS 35.0%
AGCATTTGTGTGAAAACTGT+GGG + chr1_1:4358060-4358079 Msa0002460:CDS 35.0%
!! TTTCGATCTTCAAGAGCATT+GGG + chr1_1:4358089-4358108 Msa0002460:CDS 35.0%
!!! TGTTTTAAGGTGTTTGGTTC+TGG + chr1_1:4358197-4358216 Msa0002460:CDS 35.0%
TCTTTGAATCTAGCAGCAAT+AGG - chr1_1:4358302-4358321 None:intergenic 35.0%
AGATCATCATCTTCTTCAAG+TGG - chr1_1:4358353-4358372 None:intergenic 35.0%
!! AAGCTTTGTTCAAGGACGTT+TGG + chr1_1:4357450-4357469 Msa0002460:CDS 40.0%
TCCGATGATGAGAAAGAATC+AGG - chr1_1:4357552-4357571 None:intergenic 40.0%
TTAGATCGTTGTTGACGAGT+CGG - chr1_1:4357741-4357760 None:intergenic 40.0%
CTCGTCAACAACGATCTAAA+CGG + chr1_1:4357742-4357761 Msa0002460:CDS 40.0%
! TTGTTGTCGTCGTTGTTGTT+TGG - chr1_1:4357777-4357796 None:intergenic 40.0%
! GTGAATTGTGATGGAGTTGT+TGG - chr1_1:4357849-4357868 None:intergenic 40.0%
GTATCAGAAACTGAACTCAC+AGG - chr1_1:4357882-4357901 None:intergenic 40.0%
CATAGCAACATCTTCTTCAG+GGG - chr1_1:4357907-4357926 None:intergenic 40.0%
CACATAGCAACATCTTCTTC+AGG - chr1_1:4357909-4357928 None:intergenic 40.0%
GGAACAAGATGAAGAAGAAG+AGG + chr1_1:4357986-4358005 Msa0002460:CDS 40.0%
AGATGAAGAAGAAGAGGGAT+CGG + chr1_1:4357992-4358011 Msa0002460:CDS 40.0%
ATCGGTGGAGAAAATATCGA+AGG + chr1_1:4358010-4358029 Msa0002460:CDS 40.0%
! GTTTCGATCTTCAAGAGCAT+TGG + chr1_1:4358088-4358107 Msa0002460:CDS 40.0%
GATGAAGCGAAGAATGGTAA+TGG + chr1_1:4358137-4358156 Msa0002460:CDS 40.0%
!! GCGAAGAATGGTAATGGTAA+TGG + chr1_1:4358143-4358162 Msa0002460:CDS 40.0%
!! CAGTGGAAGTTGATGAAGAA+GGG - chr1_1:4358262-4358281 None:intergenic 40.0%
!! ACAGTGGAAGTTGATGAAGA+AGG - chr1_1:4358263-4358282 None:intergenic 40.0%
!! AAGCTTTGTTCAAGGACGTT+TGG + chr1_1:4357450-4357469 Msa0002460:CDS 40.0%
TCCGATGATGAGAAAGAATC+AGG - chr1_1:4357552-4357571 None:intergenic 40.0%
TTAGATCGTTGTTGACGAGT+CGG - chr1_1:4357741-4357760 None:intergenic 40.0%
CTCGTCAACAACGATCTAAA+CGG + chr1_1:4357742-4357761 Msa0002460:CDS 40.0%
! TTGTTGTCGTCGTTGTTGTT+TGG - chr1_1:4357777-4357796 None:intergenic 40.0%
! GTGAATTGTGATGGAGTTGT+TGG - chr1_1:4357849-4357868 None:intergenic 40.0%
GTATCAGAAACTGAACTCAC+AGG - chr1_1:4357882-4357901 None:intergenic 40.0%
CATAGCAACATCTTCTTCAG+GGG - chr1_1:4357907-4357926 None:intergenic 40.0%
CACATAGCAACATCTTCTTC+AGG - chr1_1:4357909-4357928 None:intergenic 40.0%
GGAACAAGATGAAGAAGAAG+AGG + chr1_1:4357986-4358005 Msa0002460:CDS 40.0%
AGATGAAGAAGAAGAGGGAT+CGG + chr1_1:4357992-4358011 Msa0002460:CDS 40.0%
ATCGGTGGAGAAAATATCGA+AGG + chr1_1:4358010-4358029 Msa0002460:CDS 40.0%
! GTTTCGATCTTCAAGAGCAT+TGG + chr1_1:4358088-4358107 Msa0002460:CDS 40.0%
GATGAAGCGAAGAATGGTAA+TGG + chr1_1:4358137-4358156 Msa0002460:CDS 40.0%
!! GCGAAGAATGGTAATGGTAA+TGG + chr1_1:4358143-4358162 Msa0002460:CDS 40.0%
!! CAGTGGAAGTTGATGAAGAA+GGG - chr1_1:4358262-4358281 None:intergenic 40.0%
!! ACAGTGGAAGTTGATGAAGA+AGG - chr1_1:4358263-4358282 None:intergenic 40.0%
TGTTCAAGGACGTTTGGCAA+TGG + chr1_1:4357456-4357475 Msa0002460:CDS 45.0%
!! TTTGGCAATGGAAGAGCACT+TGG + chr1_1:4357468-4357487 Msa0002460:CDS 45.0%
TAGATCGTTGTTGACGAGTC+GGG - chr1_1:4357740-4357759 None:intergenic 45.0%
GAAACCAAAGCCGAGTTTCA+TGG + chr1_1:4357821-4357840 Msa0002460:CDS 45.0%
GTTGTTGGCACCATGAAACT+CGG - chr1_1:4357834-4357853 None:intergenic 45.0%
TGATGCTTTCGCGAGACAAA+TGG + chr1_1:4357934-4357953 Msa0002460:CDS 45.0%
!! TTTGGTTCTGGTCAAGCACT+TGG + chr1_1:4358209-4358228 Msa0002460:CDS 45.0%
TCATCTTCTTCAAGTGGAGC+TGG - chr1_1:4358347-4358366 None:intergenic 45.0%
TGTTCAAGGACGTTTGGCAA+TGG + chr1_1:4357456-4357475 Msa0002460:CDS 45.0%
!! TTTGGCAATGGAAGAGCACT+TGG + chr1_1:4357468-4357487 Msa0002460:CDS 45.0%
TAGATCGTTGTTGACGAGTC+GGG - chr1_1:4357740-4357759 None:intergenic 45.0%
GAAACCAAAGCCGAGTTTCA+TGG + chr1_1:4357821-4357840 Msa0002460:CDS 45.0%
GTTGTTGGCACCATGAAACT+CGG - chr1_1:4357834-4357853 None:intergenic 45.0%
TGATGCTTTCGCGAGACAAA+TGG + chr1_1:4357934-4357953 Msa0002460:CDS 45.0%
!! TTTGGTTCTGGTCAAGCACT+TGG + chr1_1:4358209-4358228 Msa0002460:CDS 45.0%
TCATCTTCTTCAAGTGGAGC+TGG - chr1_1:4358347-4358366 None:intergenic 45.0%
! AGCACTTGGTGGACACATGA+AGG + chr1_1:4357482-4357501 Msa0002460:CDS 50.0%
TGAAGAAGAAGAGGGATCGG+TGG + chr1_1:4357995-4358014 Msa0002460:CDS 50.0%
TGTCGCGATGAAGCGAAGAA+TGG + chr1_1:4358131-4358150 Msa0002460:CDS 50.0%
! AGCACTTGGTGGACACATGA+AGG + chr1_1:4357482-4357501 Msa0002460:CDS 50.0%
TGAAGAAGAAGAGGGATCGG+TGG + chr1_1:4357995-4358014 Msa0002460:CDS 50.0%
TGTCGCGATGAAGCGAAGAA+TGG + chr1_1:4358131-4358150 Msa0002460:CDS 50.0%
!! GGCAATGGAAGAGCACTTGG+TGG + chr1_1:4357471-4357490 Msa0002460:CDS 55.0%
GGCACCATGAAACTCGGCTT+TGG - chr1_1:4357828-4357847 None:intergenic 55.0%
! GGTTCTGGTCAAGCACTTGG+TGG + chr1_1:4358212-4358231 Msa0002460:CDS 55.0%
!! GGCAATGGAAGAGCACTTGG+TGG + chr1_1:4357471-4357490 Msa0002460:CDS 55.0%
GGCACCATGAAACTCGGCTT+TGG - chr1_1:4357828-4357847 None:intergenic 55.0%
! GGTTCTGGTCAAGCACTTGG+TGG + chr1_1:4358212-4358231 Msa0002460:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 4357432 4358394 4357432 ID=Msa0002460;Name=Msa0002460
chr1_1 mRNA 4357432 4358394 4357432 ID=Msa0002460-mRNA-1;Parent=Msa0002460;Name=Msa0002460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|320
chr1_1 exon 4357432 4358394 4357432 ID=Msa0002460-mRNA-1:exon:1304;Parent=Msa0002460-mRNA-1
chr1_1 CDS 4357432 4358394 4357432 ID=Msa0002460-mRNA-1:cds;Parent=Msa0002460-mRNA-1
Gene Sequence

>Msa0002460

ATGGAAAGACACAAATGCAAGCTTTGTTCAAGGACGTTTGGCAATGGAAGAGCACTTGGTGGACACATGAAGGCTCATCTTGCTATTGCTAAGTCTCAACAGAAACAATTCACTCAACCTGATTCTTTCTCATCATCGGAATCAGAACAAGAAGAAGAAGAAGAAGAAAAAACTTTGATTAATTATGTTTTGAGAGAGAATCCCAAGAAAAGTCTCAAAGTTGCAGATCCTGAATTTTCTTTTGTACGTGAAACTGAGTCTGTTATTCTTCAAGATAGAGAAAGTGAGACTGAGTCAAAGAACAACCCGACTCGTCAACAACGATCTAAACGGAACAGAAAACCAAACAACAACGACGACAACAACAACCAGAATTTTCTACAACAGAAGAAACCAAAGCCGAGTTTCATGGTGCCAACAACTCCATCACAATTCACTGAACAAGAACCTGTGAGTTCAGTTTCTGATACTTCCCCTGAAGAAGATGTTGCTATGTGTCTCATGATGCTTTCGCGAGACAAATGGAGTAGAAAGATGAACAACATCAACAATGTGGAACAAGATGAAGAAGAAGAGGGATCGGTGGAGAAAATATCGAAGGTGAAGTTATTGAAACGAGTTCGTGGGAAGCATTTGTGTGAAAACTGTGGGAAGACGTTTCGATCTTCAAGAGCATTGGGAAGTCATAGAAGTATTTGTTGTCGCGATGAAGCGAAGAATGGTAATGGTAATGGTAAAGATGATAAGATTTTTGAATGTCCATTTTGTTTTAAGGTGTTTGGTTCTGGTCAAGCACTTGGTGGTCACAAGAGATCTCATCTCATGATCCCTTCTTCATCAACTTCCACTGTTAACGTTAATGTTAATCCTATTGCTGCTAGATTCAAAGAGAGTTTCATAGATCTCAATATGCCAGCTCCACTTGAAGAAGATGATGATCTTAGTGTTGTTTCTGATGCCTAA

Protein sequence

>Msa0002460

MERHKCKLCSRTFGNGRALGGHMKAHLAIAKSQQKQFTQPDSFSSSESEQEEEEEEKTLINYVLRENPKKSLKVADPEFSFVRETESVILQDRESETESKNNPTRQQRSKRNRKPNNNDDNNNQNFLQQKKPKPSFMVPTTPSQFTEQEPVSSVSDTSPEEDVAMCLMMLSRDKWSRKMNNINNVEQDEEEEGSVEKISKVKLLKRVRGKHLCENCGKTFRSSRALGSHRSICCRDEAKNGNGNGKDDKIFECPFCFKVFGSGQALGGHKRSHLMIPSSSTSTVNVNVNPIAARFKESFIDLNMPAPLEEDDDLSVVSDA*