AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0747710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0747710 MtrunA17_Chr1g0151781 98.547 344 5 0 1 344 1 344 0.0 708
Msa0747710 MtrunA17_Chr7g0249421 46.687 332 148 4 33 335 60 391 3.67e-98 299
Msa0747710 MtrunA17_Chr4g0022171 47.284 313 151 3 33 331 61 373 8.00e-98 297
Msa0747710 MtrunA17_Chr7g0243571 46.278 309 153 3 37 332 60 368 5.84e-97 294
Msa0747710 MtrunA17_Chr2g0324061 48.077 312 149 3 33 331 55 366 3.78e-95 290
Msa0747710 MtrunA17_Chr7g0221691 47.799 318 142 6 33 328 93 408 1.02e-94 289
Msa0747710 MtrunA17_Chr4g0004971 45.902 305 155 5 40 336 49 351 2.43e-87 267
Msa0747710 MtrunA17_Chr1g0192421 44.108 297 164 2 35 329 55 351 6.54e-86 263
Msa0747710 MtrunA17_Chr8g0342431 44.578 332 141 6 33 329 50 373 8.29e-84 259
Msa0747710 MtrunA17_Chr4g0067711 41.049 324 181 6 21 337 41 361 4.05e-83 256
Msa0747710 MtrunA17_Chr2g0295401 41.875 320 178 5 21 334 42 359 6.96e-83 255
Msa0747710 MtrunA17_Chr7g0272061 42.345 307 158 5 38 329 60 362 4.89e-82 254
Msa0747710 MtrunA17_Chr4g0066371 27.304 293 170 7 51 324 68 336 1.50e-31 122
Msa0747710 MtrunA17_Chr6g0486661 29.180 305 170 8 42 324 97 377 2.97e-30 121
Msa0747710 MtrunA17_Chr3g0136711 35.398 226 115 8 114 331 82 284 2.94e-27 108
Msa0747710 MtrunA17_Chr2g0286951 28.364 275 155 5 68 324 87 337 2.07e-26 108
Msa0747710 MtrunA17_Chr1g0150451 32.911 237 119 6 70 305 110 307 6.64e-26 105
Msa0747710 MtrunA17_Chr5g0396731 34.361 227 108 8 68 288 107 298 3.47e-23 98.6
Msa0747710 MtrunA17_Chr4g0070871 32.618 233 126 8 57 287 242 445 3.90e-22 97.1
Msa0747710 MtrunA17_Chr3g0135961 32.231 242 135 8 49 287 269 484 2.20e-21 95.1
Msa0747710 MtrunA17_Chr5g0435721 31.660 259 126 9 46 290 200 421 1.07e-20 92.8
Msa0747710 MtrunA17_Chr4g0051661 29.151 271 149 9 39 307 154 383 3.72e-20 90.5
Msa0747710 MtrunA17_Chr1g0212961 30.000 240 124 8 67 305 125 321 4.57e-20 89.7
Msa0747710 MtrunA17_Chr6g0483411 31.120 241 109 6 67 288 78 280 6.87e-20 89.0
Msa0747710 MtrunA17_Chr3g0112291 32.203 236 112 10 68 290 123 323 1.28e-19 89.0
Msa0747710 MtrunA17_Chr1g0194191 30.736 231 122 9 64 288 114 312 1.14e-18 86.3
Msa0747710 MtrunA17_Chr1g0158781 30.081 246 134 7 46 288 120 330 1.45e-18 85.9
Msa0747710 MtrunA17_Chr3g0136811 28.929 280 145 10 43 288 28 287 1.52e-18 85.5
Msa0747710 MtrunA17_Chr8g0389921 31.818 220 119 7 68 287 198 386 3.15e-18 85.5
Msa0747710 MtrunA17_Chr3g0138281 28.986 276 147 10 67 335 120 353 7.58e-18 83.6
Msa0747710 MtrunA17_Chr5g0407161 29.515 227 114 9 67 288 123 308 8.87e-18 83.6
Msa0747710 MtrunA17_Chr2g0299981 29.915 234 122 6 68 283 267 476 9.82e-18 84.3
Msa0747710 MtrunA17_Chr1g0150781 31.250 224 125 8 68 287 290 488 1.26e-17 84.0
Msa0747710 MtrunA17_Chr1g0150071 28.571 280 146 10 43 288 28 287 5.61e-17 80.9
Msa0747710 MtrunA17_Chr5g0426141 30.952 252 133 10 55 305 54 265 1.57e-16 78.6
Msa0747710 MtrunA17_Chr3g0126511 30.380 237 125 6 70 305 127 324 4.56e-16 78.6
Msa0747710 MtrunA17_Chr3g0105371 30.279 251 131 12 40 287 89 298 6.63e-16 77.8
Msa0747710 MtrunA17_Chr3g0144111 33.333 165 87 5 128 287 43 189 9.19e-16 76.3
Msa0747710 MtrunA17_Chr4g0001761 27.857 280 148 10 43 288 28 287 9.22e-16 77.4
Msa0747710 MtrunA17_Chr7g0256751 29.278 263 138 9 51 288 51 290 9.39e-16 77.8
Msa0747710 MtrunA17_Chr4g0050381 29.960 247 128 13 62 305 56 260 1.04e-15 76.3
Msa0747710 MtrunA17_Chr2g0317021 29.596 223 126 10 115 329 134 333 1.12e-15 77.0
Msa0747710 MtrunA17_Chr2g0293811 26.638 229 128 8 67 288 99 294 1.13e-15 77.4
Msa0747710 MtrunA17_Chr4g0067301 28.053 303 147 11 68 310 164 455 1.95e-15 77.0
Msa0747710 MtrunA17_Chr2g0295991 27.925 265 136 9 68 288 166 419 3.68e-15 76.3
Msa0747710 MtrunA17_Chr3g0115851 28.807 243 133 9 64 305 62 265 4.15e-15 74.7
Msa0747710 MtrunA17_Chr3g0105351 30.120 249 134 11 40 287 65 274 5.05e-15 74.7
Msa0747710 MtrunA17_Chr3g0089711 29.260 311 148 14 65 330 65 348 6.49e-15 75.1
Msa0747710 MtrunA17_Chr3g0089721 29.450 309 150 14 65 330 65 348 9.11e-15 74.7
Msa0747710 MtrunA17_Chr8g0360351 28.000 250 121 11 66 305 65 265 2.34e-14 72.4
Msa0747710 MtrunA17_Chr2g0279151 27.881 269 153 10 38 305 36 264 2.59e-14 72.4
Msa0747710 MtrunA17_Chr4g0063051 25.568 352 191 13 19 337 10 323 2.60e-14 73.2
Msa0747710 MtrunA17_Chr7g0256771 28.295 258 120 12 64 288 66 291 7.61e-14 71.2
Msa0747710 MtrunA17_Chr1g0213351 27.742 310 166 13 62 340 74 356 9.78e-14 71.6
Msa0747710 MtrunA17_Chr7g0238501 24.834 302 175 11 40 312 238 516 1.34e-13 71.6
Msa0747710 MtrunA17_Chr4g0039061 26.119 268 158 8 68 312 202 452 2.72e-13 70.5
Msa0747710 MtrunA17_Chr5g0430481 29.134 254 130 10 40 287 125 334 3.50e-13 69.7
Msa0747710 MtrunA17_Chr4g0065141 23.827 277 155 8 67 332 123 354 6.41e-13 69.3
Msa0747710 MtrunA17_Chr7g0263281 27.170 265 136 10 53 288 56 292 1.37e-12 68.2
Msa0747710 MtrunA17_Chr3g0089691 30.000 230 102 10 163 343 62 281 2.83e-12 66.6
Msa0747710 MtrunA17_Chr1g0193691 31.788 151 72 4 184 312 712 853 4.38e-11 64.3
Msa0747710 MtrunA17_Chr1g0171571 26.471 272 160 10 61 329 411 645 6.68e-11 63.5
Msa0747710 MtrunA17_Chr7g0250061 28.364 275 141 12 66 312 74 320 9.77e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0747710 AT5G26010.1 53.824 340 139 8 1 333 1 329 3.70e-107 317
Msa0747710 AT4G32950.1 49.271 343 144 7 1 333 1 323 2.23e-102 304
Msa0747710 AT5G36250.1 46.061 330 151 5 33 335 62 391 1.14e-100 304
Msa0747710 AT1G16220.1 48.910 321 142 5 31 329 57 377 1.51e-99 303
Msa0747710 AT1G79630.1 44.022 368 156 5 11 336 48 407 4.33e-99 302
Msa0747710 AT1G79630.8 44.022 368 156 5 11 336 44 403 4.36e-99 302
Msa0747710 AT1G03590.1 46.398 347 166 4 1 331 23 365 5.99e-99 300
Msa0747710 AT5G36250.2 44.477 344 150 6 33 335 62 405 1.26e-96 294
Msa0747710 AT4G03415.1 48.039 306 144 5 41 331 70 375 1.74e-92 284
Msa0747710 AT4G03415.3 48.039 306 144 5 41 331 70 375 1.74e-92 284
Msa0747710 AT4G03415.2 48.039 306 144 5 41 331 70 375 1.74e-92 284
Msa0747710 AT3G02750.2 45.322 342 146 4 33 334 59 399 4.70e-92 283
Msa0747710 AT3G02750.1 45.322 342 146 4 33 334 59 399 4.70e-92 283
Msa0747710 AT1G79630.2 46.326 313 127 3 65 336 25 337 1.19e-90 278
Msa0747710 AT1G79630.7 46.326 313 127 3 65 336 25 337 1.19e-90 278
Msa0747710 AT5G01700.1 45.151 299 152 4 44 336 4 296 7.81e-88 268
Msa0747710 AT5G01700.2 44.884 303 155 4 40 336 49 345 3.29e-87 268
Msa0747710 AT3G02750.3 41.114 377 146 5 33 334 59 434 1.05e-84 266
Msa0747710 AT3G05640.2 44.407 295 155 5 38 329 62 350 3.44e-82 254
Msa0747710 AT3G05640.1 44.407 295 155 5 38 329 62 350 3.44e-82 254
Msa0747710 AT3G05640.3 44.407 295 155 5 38 329 62 350 3.44e-82 254
Msa0747710 AT1G79630.6 56.279 215 93 1 116 329 76 290 4.78e-82 254
Msa0747710 AT1G79630.3 56.279 215 93 1 116 329 76 290 4.78e-82 254
Msa0747710 AT1G79630.4 56.279 215 93 1 116 329 76 290 4.78e-82 254
Msa0747710 AT1G79630.5 56.279 215 93 1 116 329 76 290 4.78e-82 254
Msa0747710 AT5G27930.1 42.662 293 165 3 38 327 61 353 1.46e-79 248
Msa0747710 AT5G27930.2 42.662 293 165 3 38 327 61 353 1.46e-79 248
Msa0747710 AT4G32950.2 47.350 283 121 6 1 275 1 263 8.56e-78 240
Msa0747710 AT5G36250.3 44.322 273 125 5 33 278 62 334 2.70e-76 238
Msa0747710 AT3G16800.2 39.262 298 171 4 35 329 59 349 1.87e-74 234
Msa0747710 AT3G16800.1 39.262 298 171 4 35 329 59 349 1.87e-74 234
Msa0747710 AT3G16800.3 39.262 298 171 4 35 329 59 349 1.87e-74 234
Msa0747710 AT3G16800.4 38.697 261 151 3 71 329 1 254 4.45e-64 204
Msa0747710 AT3G16800.6 38.697 261 151 3 71 329 1 254 4.45e-64 204
Msa0747710 AT5G27930.3 42.739 241 135 3 38 275 61 301 1.44e-63 205
Msa0747710 AT2G20050.1 30.645 310 167 8 39 324 108 393 3.66e-34 133
Msa0747710 AT2G20050.2 30.645 310 167 8 39 324 108 393 3.83e-34 133
Msa0747710 AT3G16800.5 36.310 168 97 4 35 199 59 219 1.00e-29 114
Msa0747710 AT3G06270.1 26.756 299 176 7 51 330 70 344 4.84e-28 112
Msa0747710 AT5G10740.1 31.321 265 134 8 67 329 63 281 7.45e-26 106
Msa0747710 AT5G10740.2 31.321 265 134 8 67 329 63 281 7.45e-26 106
Msa0747710 AT4G31750.1 36.036 222 112 7 114 327 81 280 1.10e-25 105
Msa0747710 AT4G31750.3 36.036 222 112 7 114 327 81 280 1.10e-25 105
Msa0747710 AT4G31750.2 36.036 222 112 7 114 327 81 280 1.44e-25 104
Msa0747710 AT1G07430.1 31.900 279 156 11 22 290 102 356 2.45e-25 106
Msa0747710 AT5G59220.1 32.342 269 125 9 41 290 114 344 6.40e-25 104
Msa0747710 AT5G24940.1 31.061 264 134 8 68 329 64 281 1.08e-24 104
Msa0747710 AT1G43900.1 32.231 242 123 7 67 307 153 354 6.61e-22 95.9
Msa0747710 AT3G11410.1 30.855 269 126 11 46 290 112 344 2.24e-20 91.7
Msa0747710 AT1G72770.4 31.064 235 125 7 66 287 236 446 1.28e-19 90.1
Msa0747710 AT1G72770.1 31.064 235 125 7 66 287 236 446 1.28e-19 90.1
Msa0747710 AT1G72770.3 31.064 235 125 7 66 287 236 446 1.28e-19 90.1
Msa0747710 AT2G20630.2 29.577 284 134 13 65 337 60 288 2.24e-19 87.4
Msa0747710 AT2G20630.1 30.159 252 120 10 65 307 60 264 5.68e-19 85.9
Msa0747710 AT4G28400.3 29.730 259 125 10 62 311 40 250 7.38e-19 85.5
Msa0747710 AT4G28400.1 29.730 259 125 10 62 311 62 272 1.15e-18 85.1
Msa0747710 AT2G29380.1 29.297 256 152 10 41 290 80 312 2.02e-18 85.5
Msa0747710 AT5G53140.1 29.412 272 134 10 70 334 134 354 3.26e-18 85.5
Msa0747710 AT5G53140.2 29.412 272 134 10 70 334 134 354 3.26e-18 85.5
Msa0747710 AT2G33700.1 30.142 282 137 12 66 336 121 353 6.65e-18 84.3
Msa0747710 AT2G25620.1 29.137 278 141 11 67 332 127 360 8.56e-18 84.0
Msa0747710 AT2G25620.2 29.137 278 141 11 67 332 127 360 8.56e-18 84.0
Msa0747710 AT5G57050.2 30.909 220 124 6 68 287 120 311 1.16e-17 83.6
Msa0747710 AT5G57050.1 30.909 220 124 6 68 287 160 351 1.50e-17 83.6
Msa0747710 AT4G26080.1 31.797 217 116 8 68 283 172 357 1.68e-17 83.6
Msa0747710 AT5G51760.2 30.417 240 131 7 68 295 114 329 1.88e-17 83.2
Msa0747710 AT5G51760.1 30.417 240 131 7 68 295 144 359 2.11e-17 83.2
Msa0747710 AT2G34740.1 31.955 266 131 13 67 329 118 336 3.22e-17 82.0
Msa0747710 AT3G62260.3 29.310 232 125 8 64 288 23 222 4.94e-17 80.9
Msa0747710 AT1G17550.1 30.493 223 131 6 68 283 237 442 9.51e-17 81.6
Msa0747710 AT1G17550.2 30.493 223 131 6 68 283 237 442 9.51e-17 81.6
Msa0747710 AT4G31860.1 27.857 280 148 10 43 288 28 287 1.14e-16 80.5
Msa0747710 AT3G62260.2 29.310 232 125 8 64 288 113 312 1.33e-16 80.5
Msa0747710 AT3G62260.1 29.310 232 125 8 64 288 112 311 1.44e-16 80.5
Msa0747710 AT1G78200.5 32.804 189 97 5 126 305 54 221 1.89e-16 78.2
Msa0747710 AT1G78200.4 32.804 189 97 5 126 305 54 221 1.89e-16 78.2
Msa0747710 AT1G67820.2 29.766 299 170 9 41 337 87 347 2.21e-16 80.1
Msa0747710 AT1G67820.1 29.766 299 170 9 41 337 123 383 3.26e-16 79.7
Msa0747710 AT1G78200.1 33.516 182 105 5 126 305 99 266 3.42e-16 78.2
Msa0747710 AT1G78200.3 33.516 182 105 5 126 305 99 266 3.42e-16 78.2
Msa0747710 AT1G78200.2 33.516 182 105 5 126 305 99 266 3.42e-16 78.2
Msa0747710 AT1G22280.3 31.250 256 123 14 59 305 59 270 3.46e-16 78.2
Msa0747710 AT3G51470.2 27.848 237 125 8 57 288 51 246 5.67e-16 78.2
Msa0747710 AT3G51470.1 28.270 237 124 9 57 288 95 290 6.71e-16 78.2
Msa0747710 AT2G40180.1 29.368 269 142 10 28 288 118 346 9.93e-16 78.2
Msa0747710 AT3G17250.1 26.957 230 130 8 67 288 157 356 2.02e-15 77.4
Msa0747710 AT1G18030.1 29.060 234 123 5 67 288 108 310 2.05e-15 76.6
Msa0747710 AT1G18030.2 29.437 231 106 5 67 278 108 300 2.18e-15 76.3
Msa0747710 AT1G07160.1 27.891 294 170 10 40 328 124 380 7.74e-15 75.1
Msa0747710 AT2G30020.1 28.287 251 138 9 40 287 141 352 2.25e-14 73.9
Msa0747710 AT1G34750.2 28.866 194 95 7 126 305 101 265 5.61e-14 71.6
Msa0747710 AT1G34750.1 28.866 194 95 7 126 305 101 265 5.61e-14 71.6
Msa0747710 AT1G34750.3 28.866 194 95 7 126 305 101 265 5.61e-14 71.6
Msa0747710 AT1G22280.1 32.673 202 106 10 115 305 82 264 8.43e-14 71.2
Msa0747710 AT3G16560.3 25.087 287 162 9 68 312 175 450 1.89e-13 71.6
Msa0747710 AT3G16560.1 25.087 287 162 9 68 312 175 450 2.57e-13 71.2
Msa0747710 AT3G16560.4 25.087 287 162 9 68 312 175 450 2.57e-13 71.2
Msa0747710 AT3G16560.2 25.087 287 162 9 68 312 175 450 2.57e-13 71.2
Msa0747710 AT4G28400.2 30.088 226 101 10 62 278 62 239 3.94e-13 68.9
Msa0747710 AT1G48040.1 28.509 228 127 9 67 288 115 312 4.90e-13 70.1
Msa0747710 AT3G55050.1 26.207 290 158 13 50 312 68 328 1.49e-12 68.6
Msa0747710 AT3G55050.2 26.207 290 158 13 50 312 68 328 1.49e-12 68.6
Msa0747710 AT3G15260.2 29.231 195 102 8 125 307 104 274 6.12e-12 65.9
Msa0747710 AT3G15260.1 29.231 195 102 8 125 307 104 274 6.12e-12 65.9
Msa0747710 AT3G12620.2 27.500 280 137 12 66 312 81 327 7.42e-12 66.2
Msa0747710 AT3G12620.1 27.500 280 137 12 66 312 81 327 7.42e-12 66.2
Msa0747710 AT5G06750.3 26.855 283 156 10 58 312 71 330 2.06e-11 65.1
Msa0747710 AT5G06750.1 26.855 283 156 10 58 312 71 330 2.06e-11 65.1
Msa0747710 AT4G27800.2 31.282 195 88 7 126 288 131 311 4.01e-11 63.9
Msa0747710 AT4G27800.3 31.282 195 88 7 126 288 131 311 4.42e-11 63.5
Msa0747710 AT4G27800.1 31.282 195 88 7 126 288 131 311 5.76e-11 63.5
Msa0747710 AT2G34740.2 33.889 180 93 10 112 282 147 309 6.77e-11 63.2
Msa0747710 AT2G34740.3 31.193 218 108 11 67 282 114 291 7.23e-11 62.8

Find 78 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTGTGTTTATGCGTTAA+AGG 0.236530 5_2:-540498 Msa0747710:CDS
TTATTGCTACACCAGATATT+TGG 0.238563 5_2:-540376 Msa0747710:CDS
ACCACAAAACTTGGAGTATA+TGG 0.271089 5_2:-540049 Msa0747710:CDS
AAAGTCTCTTAGTCTCATTT+TGG 0.297664 5_2:+542326 None:intergenic
TCTCCTCAAGGGCATTCTTT+TGG 0.300576 5_2:+541540 None:intergenic
TATATGGACAGTGGAAAATT+AGG 0.305057 5_2:-540033 Msa0747710:CDS
CAGGCGAAGCAAAGAGAATA+AGG 0.327044 5_2:-540529 Msa0747710:CDS
TCTTGGAGCTTTCAATGTAA+TGG 0.363239 5_2:-541441 Msa0747710:CDS
CTAGCCATGTCTCGAGCTTT+CGG 0.377337 5_2:-540425 Msa0747710:CDS
GGTGACTCAAGAGCAATATT+AGG 0.378928 5_2:-541233 Msa0747710:CDS
AGATGGAAAGAAGCTATTCT+TGG 0.379447 5_2:-541458 Msa0747710:CDS
AAACGGTGATACCAAATATC+TGG 0.385349 5_2:+540365 None:intergenic
GTGGTTTCTTCTGCAGGAAA+TGG 0.397763 5_2:+540067 None:intergenic
CACTTGCAAGAACAATAAAT+TGG 0.399587 5_2:+540330 None:intergenic
AGTACCTTGTTTAGAGTAAA+CGG 0.400838 5_2:+542300 None:intergenic
TAGGCCAGGGTAGTTCTCAT+TGG 0.416310 5_2:+540445 None:intergenic
TTTCAAACAACTATCTAAGA+TGG 0.418400 5_2:-541475 Msa0747710:CDS
CAAATCAGTGGTCAATTGAA+TGG 0.418841 5_2:+541184 None:intergenic
ATTGGTCACTTGATGTTAAA+CGG 0.419251 5_2:+540348 None:intergenic
TTACTCTAAACAAGGTACTA+AGG 0.430230 5_2:-542296 Msa0747710:CDS
AACCACATGTCCAACGAGTA+TGG 0.432459 5_2:-540472 Msa0747710:CDS
GTTGCATCTTCAGAGATTCA+TGG 0.433464 5_2:-542400 Msa0747710:CDS
TTTATTGTTCTTGCAAGTGA+TGG 0.439321 5_2:-540326 Msa0747710:CDS
GCAGAAGAAACCACAAAACT+TGG 0.461758 5_2:-540058 Msa0747710:CDS
TTGACCACTGATTTGAAGCC+GGG 0.462099 5_2:-541176 Msa0747710:CDS
CTTCTTTGCCCATGCAGCTG+TGG 0.475385 5_2:+540128 None:intergenic
CATTCTCATCATGAACTTGA+GGG 0.480118 5_2:+542374 None:intergenic
AAGGATTATGGGATGAAAGA+TGG 0.481239 5_2:-541653 Msa0747710:CDS
AGGCCAGGGTAGTTCTCATT+GGG 0.484559 5_2:+540446 None:intergenic
TTATTGTTCTTGCAAGTGAT+GGG 0.484730 5_2:-540325 Msa0747710:CDS
GGAAGCAGCCACAGCTGCAT+GGG 0.485485 5_2:-540136 Msa0747710:CDS
GCTCGAGACATGGCTAGGCC+AGG 0.488145 5_2:+540431 None:intergenic
GGAAGCTGCAAGGGCAGTGG+TGG 0.495822 5_2:-540157 Msa0747710:CDS
GAGTGAAGAGGAAGCTGCAA+GGG 0.496594 5_2:-540166 Msa0747710:CDS
CAAAGAAGTTTCCTTCCTCT+AGG 0.507704 5_2:-540113 Msa0747710:CDS
GGTGAAGAGAAGCAGCATCT+TGG 0.517349 5_2:+542266 None:intergenic
AGATGCTGCTTCTCTTCACC+AGG 0.518124 5_2:-542263 Msa0747710:intron
ATTGACCACTGATTTGAAGC+CGG 0.518210 5_2:-541177 Msa0747710:CDS
TGCTGTAGTTGTCATAAGAC+AGG 0.527202 5_2:-541366 Msa0747710:intron
TGGAAGCAGCCACAGCTGCA+TGG 0.533129 5_2:-540137 Msa0747710:CDS
AATCTAGATTGTTCTTGTAG+TGG 0.537196 5_2:-541395 Msa0747710:CDS
TACTCTAAACAAGGTACTAA+GGG 0.539094 5_2:-542295 Msa0747710:CDS
AGAATCTTACAAGGCAATCC+CGG 0.541715 5_2:+541158 None:intergenic
TTGTTGTATTAATCTCCTCA+AGG 0.549692 5_2:+541528 None:intergenic
ACATTCTCATCATGAACTTG+AGG 0.551482 5_2:+542373 None:intergenic
TGTTGTATTAATCTCCTCAA+GGG 0.556478 5_2:+541529 None:intergenic
GGTCTTATCATAGTTAACCT+TGG 0.561803 5_2:-541254 Msa0747710:CDS
AAAGAAGTTTCCTTCCTCTA+GGG 0.562358 5_2:-540112 Msa0747710:CDS
AACCATACTCGTTGGACATG+TGG 0.563398 5_2:+540470 None:intergenic
CATTGGGCAACCATACTCGT+TGG 0.565790 5_2:+540462 None:intergenic
CGAAAGCTCGAGACATGGCT+AGG 0.567459 5_2:+540426 None:intergenic
TTTCAATGTAATGGATGAAG+AGG 0.572129 5_2:-541432 Msa0747710:CDS
CATGATATTTGAAGCAAGCA+AGG 0.574257 5_2:-542350 Msa0747710:CDS
AGAGGAAGCTGCAAGGGCAG+TGG 0.574488 5_2:-540160 Msa0747710:CDS
CTCGAGACATGGCTAGGCCA+GGG 0.576527 5_2:+540432 None:intergenic
TTGCCCAATGAGAACTACCC+TGG 0.599999 5_2:-540449 Msa0747710:CDS
CAATCATCTACCCTAGAGGA+AGG 0.600898 5_2:+540102 None:intergenic
ATGAATCTCTGAAGATGCAA+CGG 0.607268 5_2:+542402 None:intergenic
GAGCCAAAAGAATGCCCTTG+AGG 0.608848 5_2:-541543 Msa0747710:CDS
AGAGTGAAGAGGAAGCTGCA+AGG 0.613435 5_2:-540167 Msa0747710:CDS
TGCTCCGTTTACTCTAAACA+AGG 0.613627 5_2:-542304 Msa0747710:CDS
TATTGTTCTTGCAAGTGATG+GGG 0.615238 5_2:-540324 Msa0747710:intron
ATCTCCGAAAGCTCGAGACA+TGG 0.616410 5_2:+540421 None:intergenic
GAACAATATAAACAGGGTGA+AGG 0.617168 5_2:-541275 Msa0747710:intron
TGTGGTGTTTATGATGGACA+TGG 0.621779 5_2:-541623 Msa0747710:CDS
GGTTGCATCAATTGTGTGGA+TGG 0.622975 5_2:-540193 Msa0747710:CDS
GATTTCATACTTAAAGACCA+TGG 0.635074 5_2:-540401 Msa0747710:CDS
CAATCCCGGCTTCAAATCAG+TGG 0.636969 5_2:+541172 None:intergenic
GTGGATGGTAGAGAGTGAAG+AGG 0.644994 5_2:-540178 Msa0747710:CDS
AAGAGGTTGCATCAATTGTG+TGG 0.658363 5_2:-540197 Msa0747710:CDS
AAGAGAATAAGGAGTTGCAA+CGG 0.664540 5_2:-540518 Msa0747710:CDS
AACTTGGAGTATATGGACAG+TGG 0.675658 5_2:-540042 Msa0747710:CDS
GGATGTACTAAGTAATGAAG+AGG 0.676441 5_2:-540214 Msa0747710:CDS
ATATTGCTCTTGAGTCACCA+AGG 0.689464 5_2:+541237 None:intergenic
CTGCAGGAAATGGCAAACCA+CGG 0.691531 5_2:+540077 None:intergenic
GGTGCAATCATCTACCCTAG+AGG 0.703711 5_2:+540098 None:intergenic
CTGGTGTAGCAATAACACCA+TGG 0.708013 5_2:+540384 None:intergenic
TAGGGTAGATGATTGCACCG+TGG 0.761662 5_2:-540094 Msa0747710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAATATTATTATATT+TGG + chr5_2:541671-541690 None:intergenic 0.0%
!! TATTAAAATATTATTATATT+TGG + chr5_2:541671-541690 None:intergenic 0.0%
!! TATCTTAAATTACTAAAAAA+AGG + chr5_2:540570-540589 None:intergenic 10.0%
!!! ATCATTTTCTAAAAAAAAAA+AGG - chr5_2:541603-541622 Msa0747710:CDS 10.0%
!! TATCTTAAATTACTAAAAAA+AGG + chr5_2:540570-540589 None:intergenic 10.0%
!!! ATCATTTTCTAAAAAAAAAA+AGG - chr5_2:541603-541622 Msa0747710:CDS 10.0%
!! CAAAAAAAAAAAAACTTTGA+TGG - chr5_2:540520-540539 Msa0747710:CDS 15.0%
!!! AATTTAAGATATCATGATTA+TGG - chr5_2:540579-540598 Msa0747710:intron 15.0%
!!! ATTTTCTAAAAAAAAAAAGG+TGG - chr5_2:541606-541625 Msa0747710:CDS 15.0%
!! TATTTGTATCTAAACTAAAT+TGG - chr5_2:541891-541910 Msa0747710:intron 15.0%
!! CAAAAAAAAAAAAACTTTGA+TGG - chr5_2:540520-540539 Msa0747710:CDS 15.0%
!!! AATTTAAGATATCATGATTA+TGG - chr5_2:540579-540598 Msa0747710:intron 15.0%
!!! ATTTTCTAAAAAAAAAAAGG+TGG - chr5_2:541606-541625 Msa0747710:CDS 15.0%
!! TATTTGTATCTAAACTAAAT+TGG - chr5_2:541891-541910 Msa0747710:intron 15.0%
!!! TAACCTTTAAATAATTTGCA+TGG - chr5_2:540226-540245 Msa0747710:intron 20.0%
!!! AATTTGAAGATAATTTTGCA+TGG - chr5_2:540365-540384 Msa0747710:CDS 20.0%
!! TGTTGTGATCTTTAATATTA+AGG - chr5_2:540423-540442 Msa0747710:CDS 20.0%
!! AAGTACATGACTTTATAAAA+AGG + chr5_2:540702-540721 None:intergenic 20.0%
!! TTAAGGAAAAAGAAACAAAA+GGG + chr5_2:540774-540793 None:intergenic 20.0%
!! TTTAAGGAAAAAGAAACAAA+AGG + chr5_2:540775-540794 None:intergenic 20.0%
!!! TTTGTTTCTTTTTCCTTAAA+AGG - chr5_2:540775-540794 Msa0747710:intron 20.0%
!!! TTTTTCCTTAAAAGGATTAT+GGG - chr5_2:540783-540802 Msa0747710:intron 20.0%
!! GAGATTAATACAACAAAAAA+TGG - chr5_2:540926-540945 Msa0747710:intron 20.0%
!! ATAACAAGAACAATATAAAC+AGG - chr5_2:541165-541184 Msa0747710:intron 20.0%
!! TAACAAGAACAATATAAACA+GGG - chr5_2:541166-541185 Msa0747710:intron 20.0%
!! TTCATATAACTGTTAAACTA+AGG + chr5_2:541528-541547 None:intergenic 20.0%
!! ATATTTGGATTAAAATACGT+CGG + chr5_2:541656-541675 None:intergenic 20.0%
!!! AATGGCTTTATCATTATTTA+TGG - chr5_2:542188-542207 Msa0747710:intron 20.0%
!!! TATGGATATTTTTTGCTTAT+AGG - chr5_2:542206-542225 Msa0747710:intron 20.0%
!!! TAACCTTTAAATAATTTGCA+TGG - chr5_2:540226-540245 Msa0747710:intron 20.0%
!!! AATTTGAAGATAATTTTGCA+TGG - chr5_2:540365-540384 Msa0747710:CDS 20.0%
!! TGTTGTGATCTTTAATATTA+AGG - chr5_2:540423-540442 Msa0747710:CDS 20.0%
!! AAGTACATGACTTTATAAAA+AGG + chr5_2:540702-540721 None:intergenic 20.0%
!! TTAAGGAAAAAGAAACAAAA+GGG + chr5_2:540774-540793 None:intergenic 20.0%
!! TTTAAGGAAAAAGAAACAAA+AGG + chr5_2:540775-540794 None:intergenic 20.0%
!!! TTTGTTTCTTTTTCCTTAAA+AGG - chr5_2:540775-540794 Msa0747710:intron 20.0%
!!! TTTTTCCTTAAAAGGATTAT+GGG - chr5_2:540783-540802 Msa0747710:intron 20.0%
!! GAGATTAATACAACAAAAAA+TGG - chr5_2:540926-540945 Msa0747710:intron 20.0%
!! ATAACAAGAACAATATAAAC+AGG - chr5_2:541165-541184 Msa0747710:intron 20.0%
!! TAACAAGAACAATATAAACA+GGG - chr5_2:541166-541185 Msa0747710:intron 20.0%
!! TTCATATAACTGTTAAACTA+AGG + chr5_2:541528-541547 None:intergenic 20.0%
!! ATATTTGGATTAAAATACGT+CGG + chr5_2:541656-541675 None:intergenic 20.0%
!!! AATGGCTTTATCATTATTTA+TGG - chr5_2:542188-542207 Msa0747710:intron 20.0%
!!! TATGGATATTTTTTGCTTAT+AGG - chr5_2:542206-542225 Msa0747710:intron 20.0%
!! CTTTTTCCTTAAAAGGATTA+TGG - chr5_2:540782-540801 Msa0747710:intron 25.0%
! TTTCAAACAACTATCTAAGA+TGG - chr5_2:540972-540991 Msa0747710:intron 25.0%
! TTTATGAGAAGAATCTTACA+AGG + chr5_2:541301-541320 None:intergenic 25.0%
!!! CTTTTCAATTATTGGTTTGA+CGG - chr5_2:541335-541354 Msa0747710:intron 25.0%
!!! ATATTTTTTGCTTATAGGTG+TGG - chr5_2:542211-542230 Msa0747710:intron 25.0%
!!! TATTTTTTGCTTATAGGTGT+GGG - chr5_2:542212-542231 Msa0747710:intron 25.0%
!! CTTTTTCCTTAAAAGGATTA+TGG - chr5_2:540782-540801 Msa0747710:intron 25.0%
! TTTCAAACAACTATCTAAGA+TGG - chr5_2:540972-540991 Msa0747710:intron 25.0%
! TTTATGAGAAGAATCTTACA+AGG + chr5_2:541301-541320 None:intergenic 25.0%
!!! CTTTTCAATTATTGGTTTGA+CGG - chr5_2:541335-541354 Msa0747710:intron 25.0%
!!! ATATTTTTTGCTTATAGGTG+TGG - chr5_2:542211-542230 Msa0747710:intron 25.0%
!!! TATTTTTTGCTTATAGGTGT+GGG - chr5_2:542212-542231 Msa0747710:intron 25.0%
! AAAGTCTCTTAGTCTCATTT+TGG + chr5_2:540124-540143 None:intergenic 30.0%
! AGTACCTTGTTTAGAGTAAA+CGG + chr5_2:540150-540169 None:intergenic 30.0%
! TTACTCTAAACAAGGTACTA+AGG - chr5_2:540151-540170 Msa0747710:CDS 30.0%
! TACTCTAAACAAGGTACTAA+GGG - chr5_2:540152-540171 Msa0747710:CDS 30.0%
TGGCCATGCAAATTATTTAA+AGG + chr5_2:540232-540251 None:intergenic 30.0%
ATGAGAGAATTAAAACCTTG+TGG + chr5_2:540252-540271 None:intergenic 30.0%
!!! ACAAGGTTTTAATTCTCTCA+TGG - chr5_2:540251-540270 Msa0747710:intron 30.0%
TCTTCAAATTCTACACAACT+TGG + chr5_2:540355-540374 None:intergenic 30.0%
CAATCTGAGTCAAGATATTA+AGG + chr5_2:540669-540688 None:intergenic 30.0%
AGAAACAAAAGGGAGATATA+TGG + chr5_2:540764-540783 None:intergenic 30.0%
! TTCATCCCATAATCCTTTTA+AGG + chr5_2:540791-540810 None:intergenic 30.0%
!! TATGATGGACATGGAAAAAA+TGG - chr5_2:540833-540852 Msa0747710:intron 30.0%
TGTTGTATTAATCTCCTCAA+GGG + chr5_2:540921-540940 None:intergenic 30.0%
TTGTTGTATTAATCTCCTCA+AGG + chr5_2:540922-540941 None:intergenic 30.0%
!! TTTCAATGTAATGGATGAAG+AGG - chr5_2:541015-541034 Msa0747710:intron 30.0%
AATCTAGATTGTTCTTGTAG+TGG - chr5_2:541052-541071 Msa0747710:intron 30.0%
!!! TGGCTTTTAGTTTTTCATCT+TGG + chr5_2:541246-541265 None:intergenic 30.0%
ATAATTGAAAAGAAGCTGCA+TGG + chr5_2:541327-541346 None:intergenic 30.0%
! TGCAGCTTCTTTTCAATTAT+TGG - chr5_2:541327-541346 Msa0747710:intron 30.0%
AACTGTTAAACTAAGGTGTA+TGG + chr5_2:541521-541540 None:intergenic 30.0%
CAAATAAGAGACTTCTAGAT+AGG - chr5_2:541577-541596 Msa0747710:CDS 30.0%
! TGTGTCATTATTGACGTTTA+AGG + chr5_2:541726-541745 None:intergenic 30.0%
TTAAACGTCAATAATGACAC+AGG - chr5_2:541725-541744 Msa0747710:intron 30.0%
TAAACGTCAATAATGACACA+GGG - chr5_2:541726-541745 Msa0747710:intron 30.0%
TCTAAACTAAATTGGAATGC+AGG - chr5_2:541899-541918 Msa0747710:intron 30.0%
GATTTCATACTTAAAGACCA+TGG - chr5_2:542046-542065 Msa0747710:intron 30.0%
TTATTGCTACACCAGATATT+TGG - chr5_2:542071-542090 Msa0747710:intron 30.0%
ATTGGTCACTTGATGTTAAA+CGG + chr5_2:542102-542121 None:intergenic 30.0%
CACTTGCAAGAACAATAAAT+TGG + chr5_2:542120-542139 None:intergenic 30.0%
TTTATTGTTCTTGCAAGTGA+TGG - chr5_2:542121-542140 Msa0747710:intron 30.0%
TTATTGTTCTTGCAAGTGAT+GGG - chr5_2:542122-542141 Msa0747710:intron 30.0%
CTTGCATACAACAATTTCAA+TGG - chr5_2:542170-542189 Msa0747710:intron 30.0%
TATATGGACAGTGGAAAATT+AGG - chr5_2:542414-542433 Msa0747710:CDS 30.0%
! AAAGTCTCTTAGTCTCATTT+TGG + chr5_2:540124-540143 None:intergenic 30.0%
! AGTACCTTGTTTAGAGTAAA+CGG + chr5_2:540150-540169 None:intergenic 30.0%
! TTACTCTAAACAAGGTACTA+AGG - chr5_2:540151-540170 Msa0747710:CDS 30.0%
! TACTCTAAACAAGGTACTAA+GGG - chr5_2:540152-540171 Msa0747710:CDS 30.0%
TGGCCATGCAAATTATTTAA+AGG + chr5_2:540232-540251 None:intergenic 30.0%
ATGAGAGAATTAAAACCTTG+TGG + chr5_2:540252-540271 None:intergenic 30.0%
!!! ACAAGGTTTTAATTCTCTCA+TGG - chr5_2:540251-540270 Msa0747710:intron 30.0%
TCTTCAAATTCTACACAACT+TGG + chr5_2:540355-540374 None:intergenic 30.0%
CAATCTGAGTCAAGATATTA+AGG + chr5_2:540669-540688 None:intergenic 30.0%
AGAAACAAAAGGGAGATATA+TGG + chr5_2:540764-540783 None:intergenic 30.0%
! TTCATCCCATAATCCTTTTA+AGG + chr5_2:540791-540810 None:intergenic 30.0%
!! TATGATGGACATGGAAAAAA+TGG - chr5_2:540833-540852 Msa0747710:intron 30.0%
TGTTGTATTAATCTCCTCAA+GGG + chr5_2:540921-540940 None:intergenic 30.0%
TTGTTGTATTAATCTCCTCA+AGG + chr5_2:540922-540941 None:intergenic 30.0%
!! TTTCAATGTAATGGATGAAG+AGG - chr5_2:541015-541034 Msa0747710:intron 30.0%
AATCTAGATTGTTCTTGTAG+TGG - chr5_2:541052-541071 Msa0747710:intron 30.0%
!!! TGGCTTTTAGTTTTTCATCT+TGG + chr5_2:541246-541265 None:intergenic 30.0%
ATAATTGAAAAGAAGCTGCA+TGG + chr5_2:541327-541346 None:intergenic 30.0%
! TGCAGCTTCTTTTCAATTAT+TGG - chr5_2:541327-541346 Msa0747710:intron 30.0%
AACTGTTAAACTAAGGTGTA+TGG + chr5_2:541521-541540 None:intergenic 30.0%
CAAATAAGAGACTTCTAGAT+AGG - chr5_2:541577-541596 Msa0747710:CDS 30.0%
! TGTGTCATTATTGACGTTTA+AGG + chr5_2:541726-541745 None:intergenic 30.0%
TTAAACGTCAATAATGACAC+AGG - chr5_2:541725-541744 Msa0747710:intron 30.0%
TAAACGTCAATAATGACACA+GGG - chr5_2:541726-541745 Msa0747710:intron 30.0%
TCTAAACTAAATTGGAATGC+AGG - chr5_2:541899-541918 Msa0747710:intron 30.0%
GATTTCATACTTAAAGACCA+TGG - chr5_2:542046-542065 Msa0747710:intron 30.0%
TTATTGCTACACCAGATATT+TGG - chr5_2:542071-542090 Msa0747710:intron 30.0%
ATTGGTCACTTGATGTTAAA+CGG + chr5_2:542102-542121 None:intergenic 30.0%
CACTTGCAAGAACAATAAAT+TGG + chr5_2:542120-542139 None:intergenic 30.0%
TTTATTGTTCTTGCAAGTGA+TGG - chr5_2:542121-542140 Msa0747710:intron 30.0%
TTATTGTTCTTGCAAGTGAT+GGG - chr5_2:542122-542141 Msa0747710:intron 30.0%
CTTGCATACAACAATTTCAA+TGG - chr5_2:542170-542189 Msa0747710:intron 30.0%
TATATGGACAGTGGAAAATT+AGG - chr5_2:542414-542433 Msa0747710:CDS 30.0%
! ATGAATCTCTGAAGATGCAA+CGG + chr5_2:540048-540067 None:intergenic 35.0%
CATTCTCATCATGAACTTGA+GGG + chr5_2:540076-540095 None:intergenic 35.0%
ACATTCTCATCATGAACTTG+AGG + chr5_2:540077-540096 None:intergenic 35.0%
! CATGATATTTGAAGCAAGCA+AGG - chr5_2:540097-540116 Msa0747710:CDS 35.0%
! AAATAATTTGCATGGCCACA+AGG - chr5_2:540234-540253 Msa0747710:intron 35.0%
ATGGTAATCACGAACAAATG+TGG - chr5_2:540598-540617 Msa0747710:intron 35.0%
AAGGATTATGGGATGAAAGA+TGG - chr5_2:540794-540813 Msa0747710:intron 35.0%
!! ATGAAAGATGGAGCATTTTG+TGG - chr5_2:540806-540825 Msa0747710:intron 35.0%
! GCATTTTGTGGTGTTTATGA+TGG - chr5_2:540818-540837 Msa0747710:intron 35.0%
!!! TTTTGGCTCAAAATGAGAGA+TGG + chr5_2:540893-540912 None:intergenic 35.0%
AGATGGAAAGAAGCTATTCT+TGG - chr5_2:540989-541008 Msa0747710:intron 35.0%
TCTTGGAGCTTTCAATGTAA+TGG - chr5_2:541006-541025 Msa0747710:intron 35.0%
GAACAATATAAACAGGGTGA+AGG - chr5_2:541172-541191 Msa0747710:CDS 35.0%
! GGTCTTATCATAGTTAACCT+TGG - chr5_2:541193-541212 Msa0747710:CDS 35.0%
CAAATCAGTGGTCAATTGAA+TGG + chr5_2:541266-541285 None:intergenic 35.0%
ATAAGAGACTTCTAGATAGG+TGG - chr5_2:541580-541599 Msa0747710:CDS 35.0%
!! AAGAGCGATGCTATTTCTAA+CGG + chr5_2:541867-541886 None:intergenic 35.0%
AAGAGAATAAGGAGTTGCAA+CGG - chr5_2:541929-541948 Msa0747710:intron 35.0%
AAACGGTGATACCAAATATC+TGG + chr5_2:542085-542104 None:intergenic 35.0%
TATTGTTCTTGCAAGTGATG+GGG - chr5_2:542123-542142 Msa0747710:intron 35.0%
GGATGTACTAAGTAATGAAG+AGG - chr5_2:542233-542252 Msa0747710:intron 35.0%
AAAGAAGTTTCCTTCCTCTA+GGG - chr5_2:542335-542354 Msa0747710:CDS 35.0%
ACCACAAAACTTGGAGTATA+TGG - chr5_2:542398-542417 Msa0747710:CDS 35.0%
! TCCATATACTCCAAGTTTTG+TGG + chr5_2:542402-542421 None:intergenic 35.0%
! ATGAATCTCTGAAGATGCAA+CGG + chr5_2:540048-540067 None:intergenic 35.0%
CATTCTCATCATGAACTTGA+GGG + chr5_2:540076-540095 None:intergenic 35.0%
ACATTCTCATCATGAACTTG+AGG + chr5_2:540077-540096 None:intergenic 35.0%
! CATGATATTTGAAGCAAGCA+AGG - chr5_2:540097-540116 Msa0747710:CDS 35.0%
! AAATAATTTGCATGGCCACA+AGG - chr5_2:540234-540253 Msa0747710:intron 35.0%
ATGGTAATCACGAACAAATG+TGG - chr5_2:540598-540617 Msa0747710:intron 35.0%
AAGGATTATGGGATGAAAGA+TGG - chr5_2:540794-540813 Msa0747710:intron 35.0%
!! ATGAAAGATGGAGCATTTTG+TGG - chr5_2:540806-540825 Msa0747710:intron 35.0%
! GCATTTTGTGGTGTTTATGA+TGG - chr5_2:540818-540837 Msa0747710:intron 35.0%
!!! TTTTGGCTCAAAATGAGAGA+TGG + chr5_2:540893-540912 None:intergenic 35.0%
AGATGGAAAGAAGCTATTCT+TGG - chr5_2:540989-541008 Msa0747710:intron 35.0%
TCTTGGAGCTTTCAATGTAA+TGG - chr5_2:541006-541025 Msa0747710:intron 35.0%
GAACAATATAAACAGGGTGA+AGG - chr5_2:541172-541191 Msa0747710:CDS 35.0%
! GGTCTTATCATAGTTAACCT+TGG - chr5_2:541193-541212 Msa0747710:CDS 35.0%
CAAATCAGTGGTCAATTGAA+TGG + chr5_2:541266-541285 None:intergenic 35.0%
ATAAGAGACTTCTAGATAGG+TGG - chr5_2:541580-541599 Msa0747710:CDS 35.0%
!! AAGAGCGATGCTATTTCTAA+CGG + chr5_2:541867-541886 None:intergenic 35.0%
AAGAGAATAAGGAGTTGCAA+CGG - chr5_2:541929-541948 Msa0747710:intron 35.0%
AAACGGTGATACCAAATATC+TGG + chr5_2:542085-542104 None:intergenic 35.0%
TATTGTTCTTGCAAGTGATG+GGG - chr5_2:542123-542142 Msa0747710:intron 35.0%
GGATGTACTAAGTAATGAAG+AGG - chr5_2:542233-542252 Msa0747710:intron 35.0%
AAAGAAGTTTCCTTCCTCTA+GGG - chr5_2:542335-542354 Msa0747710:CDS 35.0%
ACCACAAAACTTGGAGTATA+TGG - chr5_2:542398-542417 Msa0747710:CDS 35.0%
! TCCATATACTCCAAGTTTTG+TGG + chr5_2:542402-542421 None:intergenic 35.0%
GTTGCATCTTCAGAGATTCA+TGG - chr5_2:540047-540066 Msa0747710:CDS 40.0%
TGCTCCGTTTACTCTAAACA+AGG - chr5_2:540143-540162 Msa0747710:CDS 40.0%
TCATGATTGAGAGACAAACC+TGG + chr5_2:540205-540224 None:intergenic 40.0%
GCAACATTAGTCGCATTTGA+TGG + chr5_2:540316-540335 None:intergenic 40.0%
! TTTTGCATGGCTACACTACA+AGG - chr5_2:540378-540397 Msa0747710:CDS 40.0%
ATTTCTTGCTTGAATCGCGT+TGG - chr5_2:540624-540643 Msa0747710:intron 40.0%
!! TGTGGTGTTTATGATGGACA+TGG - chr5_2:540824-540843 Msa0747710:intron 40.0%
TGCTGTAGTTGTCATAAGAC+AGG - chr5_2:541081-541100 Msa0747710:intron 40.0%
ATATTGCTCTTGAGTCACCA+AGG + chr5_2:541213-541232 None:intergenic 40.0%
GGTGACTCAAGAGCAATATT+AGG - chr5_2:541214-541233 Msa0747710:CDS 40.0%
!! ATTGACCACTGATTTGAAGC+CGG - chr5_2:541270-541289 Msa0747710:intron 40.0%
AGAATCTTACAAGGCAATCC+CGG + chr5_2:541292-541311 None:intergenic 40.0%
TTAAGCTGCTGATGTCTTCA+AGG - chr5_2:541481-541500 Msa0747710:CDS 40.0%
! ACTAGTCTATCGTGATGCTT+TGG + chr5_2:541813-541832 None:intergenic 40.0%
ATAGACTAGTGAAACTGCAG+CGG - chr5_2:541823-541842 Msa0747710:intron 40.0%
CGGTTGTGTTTATGCGTTAA+AGG - chr5_2:541949-541968 Msa0747710:intron 40.0%
AAGAGGTTGCATCAATTGTG+TGG - chr5_2:542250-542269 Msa0747710:intron 40.0%
CAAAGAAGTTTCCTTCCTCT+AGG - chr5_2:542334-542353 Msa0747710:CDS 40.0%
!! AGTTTTGTGGTTTCTTCTGC+AGG + chr5_2:542389-542408 None:intergenic 40.0%
GCAGAAGAAACCACAAAACT+TGG - chr5_2:542389-542408 Msa0747710:CDS 40.0%
AACTTGGAGTATATGGACAG+TGG - chr5_2:542405-542424 Msa0747710:CDS 40.0%
GTTGCATCTTCAGAGATTCA+TGG - chr5_2:540047-540066 Msa0747710:CDS 40.0%
TGCTCCGTTTACTCTAAACA+AGG - chr5_2:540143-540162 Msa0747710:CDS 40.0%
TCATGATTGAGAGACAAACC+TGG + chr5_2:540205-540224 None:intergenic 40.0%
GCAACATTAGTCGCATTTGA+TGG + chr5_2:540316-540335 None:intergenic 40.0%
! TTTTGCATGGCTACACTACA+AGG - chr5_2:540378-540397 Msa0747710:CDS 40.0%
ATTTCTTGCTTGAATCGCGT+TGG - chr5_2:540624-540643 Msa0747710:intron 40.0%
!! TGTGGTGTTTATGATGGACA+TGG - chr5_2:540824-540843 Msa0747710:intron 40.0%
TGCTGTAGTTGTCATAAGAC+AGG - chr5_2:541081-541100 Msa0747710:intron 40.0%
ATATTGCTCTTGAGTCACCA+AGG + chr5_2:541213-541232 None:intergenic 40.0%
GGTGACTCAAGAGCAATATT+AGG - chr5_2:541214-541233 Msa0747710:CDS 40.0%
!! ATTGACCACTGATTTGAAGC+CGG - chr5_2:541270-541289 Msa0747710:intron 40.0%
AGAATCTTACAAGGCAATCC+CGG + chr5_2:541292-541311 None:intergenic 40.0%
TTAAGCTGCTGATGTCTTCA+AGG - chr5_2:541481-541500 Msa0747710:CDS 40.0%
! ACTAGTCTATCGTGATGCTT+TGG + chr5_2:541813-541832 None:intergenic 40.0%
ATAGACTAGTGAAACTGCAG+CGG - chr5_2:541823-541842 Msa0747710:intron 40.0%
CGGTTGTGTTTATGCGTTAA+AGG - chr5_2:541949-541968 Msa0747710:intron 40.0%
AAGAGGTTGCATCAATTGTG+TGG - chr5_2:542250-542269 Msa0747710:intron 40.0%
CAAAGAAGTTTCCTTCCTCT+AGG - chr5_2:542334-542353 Msa0747710:CDS 40.0%
!! AGTTTTGTGGTTTCTTCTGC+AGG + chr5_2:542389-542408 None:intergenic 40.0%
GCAGAAGAAACCACAAAACT+TGG - chr5_2:542389-542408 Msa0747710:CDS 40.0%
AACTTGGAGTATATGGACAG+TGG - chr5_2:542405-542424 Msa0747710:CDS 40.0%
!!! TCTCCTCAAGGGCATTCTTT+TGG + chr5_2:540910-540929 None:intergenic 45.0%
!! TTGACCACTGATTTGAAGCC+GGG - chr5_2:541271-541290 Msa0747710:intron 45.0%
CAGGCGAAGCAAAGAGAATA+AGG - chr5_2:541918-541937 Msa0747710:intron 45.0%
AACCACATGTCCAACGAGTA+TGG - chr5_2:541975-541994 Msa0747710:intron 45.0%
AACCATACTCGTTGGACATG+TGG + chr5_2:541980-541999 None:intergenic 45.0%
CTGGTGTAGCAATAACACCA+TGG + chr5_2:542066-542085 None:intergenic 45.0%
GGTTGCATCAATTGTGTGGA+TGG - chr5_2:542254-542273 Msa0747710:intron 45.0%
CAATCATCTACCCTAGAGGA+AGG + chr5_2:542348-542367 None:intergenic 45.0%
GTGGTTTCTTCTGCAGGAAA+TGG + chr5_2:542383-542402 None:intergenic 45.0%
!!! TCTCCTCAAGGGCATTCTTT+TGG + chr5_2:540910-540929 None:intergenic 45.0%
!! TTGACCACTGATTTGAAGCC+GGG - chr5_2:541271-541290 Msa0747710:intron 45.0%
CAGGCGAAGCAAAGAGAATA+AGG - chr5_2:541918-541937 Msa0747710:intron 45.0%
AACCACATGTCCAACGAGTA+TGG - chr5_2:541975-541994 Msa0747710:intron 45.0%
AACCATACTCGTTGGACATG+TGG + chr5_2:541980-541999 None:intergenic 45.0%
CTGGTGTAGCAATAACACCA+TGG + chr5_2:542066-542085 None:intergenic 45.0%
GGTTGCATCAATTGTGTGGA+TGG - chr5_2:542254-542273 Msa0747710:intron 45.0%
CAATCATCTACCCTAGAGGA+AGG + chr5_2:542348-542367 None:intergenic 45.0%
GTGGTTTCTTCTGCAGGAAA+TGG + chr5_2:542383-542402 None:intergenic 45.0%
GGTGAAGAGAAGCAGCATCT+TGG + chr5_2:540184-540203 None:intergenic 50.0%
! AGATGCTGCTTCTCTTCACC+AGG - chr5_2:540184-540203 Msa0747710:CDS 50.0%
GAGCCAAAAGAATGCCCTTG+AGG - chr5_2:540904-540923 Msa0747710:intron 50.0%
CAATCCCGGCTTCAAATCAG+TGG + chr5_2:541278-541297 None:intergenic 50.0%
CATTGGGCAACCATACTCGT+TGG + chr5_2:541988-542007 None:intergenic 50.0%
TTGCCCAATGAGAACTACCC+TGG - chr5_2:541998-542017 Msa0747710:intron 50.0%
AGGCCAGGGTAGTTCTCATT+GGG + chr5_2:542004-542023 None:intergenic 50.0%
TAGGCCAGGGTAGTTCTCAT+TGG + chr5_2:542005-542024 None:intergenic 50.0%
CTAGCCATGTCTCGAGCTTT+CGG - chr5_2:542022-542041 Msa0747710:intron 50.0%
ATCTCCGAAAGCTCGAGACA+TGG + chr5_2:542029-542048 None:intergenic 50.0%
GTGGATGGTAGAGAGTGAAG+AGG - chr5_2:542269-542288 Msa0747710:CDS 50.0%
AGAGTGAAGAGGAAGCTGCA+AGG - chr5_2:542280-542299 Msa0747710:CDS 50.0%
GAGTGAAGAGGAAGCTGCAA+GGG - chr5_2:542281-542300 Msa0747710:CDS 50.0%
GGTGCAATCATCTACCCTAG+AGG + chr5_2:542352-542371 None:intergenic 50.0%
TAGGGTAGATGATTGCACCG+TGG - chr5_2:542353-542372 Msa0747710:CDS 50.0%
CTGCAGGAAATGGCAAACCA+CGG + chr5_2:542373-542392 None:intergenic 50.0%
GGTGAAGAGAAGCAGCATCT+TGG + chr5_2:540184-540203 None:intergenic 50.0%
! AGATGCTGCTTCTCTTCACC+AGG - chr5_2:540184-540203 Msa0747710:CDS 50.0%
GAGCCAAAAGAATGCCCTTG+AGG - chr5_2:540904-540923 Msa0747710:intron 50.0%
CAATCCCGGCTTCAAATCAG+TGG + chr5_2:541278-541297 None:intergenic 50.0%
CATTGGGCAACCATACTCGT+TGG + chr5_2:541988-542007 None:intergenic 50.0%
TTGCCCAATGAGAACTACCC+TGG - chr5_2:541998-542017 Msa0747710:intron 50.0%
AGGCCAGGGTAGTTCTCATT+GGG + chr5_2:542004-542023 None:intergenic 50.0%
TAGGCCAGGGTAGTTCTCAT+TGG + chr5_2:542005-542024 None:intergenic 50.0%
CTAGCCATGTCTCGAGCTTT+CGG - chr5_2:542022-542041 Msa0747710:intron 50.0%
ATCTCCGAAAGCTCGAGACA+TGG + chr5_2:542029-542048 None:intergenic 50.0%
GTGGATGGTAGAGAGTGAAG+AGG - chr5_2:542269-542288 Msa0747710:CDS 50.0%
AGAGTGAAGAGGAAGCTGCA+AGG - chr5_2:542280-542299 Msa0747710:CDS 50.0%
GAGTGAAGAGGAAGCTGCAA+GGG - chr5_2:542281-542300 Msa0747710:CDS 50.0%
GGTGCAATCATCTACCCTAG+AGG + chr5_2:542352-542371 None:intergenic 50.0%
TAGGGTAGATGATTGCACCG+TGG - chr5_2:542353-542372 Msa0747710:CDS 50.0%
CTGCAGGAAATGGCAAACCA+CGG + chr5_2:542373-542392 None:intergenic 50.0%
CGAAAGCTCGAGACATGGCT+AGG + chr5_2:542024-542043 None:intergenic 55.0%
CTTCTTTGCCCATGCAGCTG+TGG + chr5_2:542322-542341 None:intergenic 55.0%
CGAAAGCTCGAGACATGGCT+AGG + chr5_2:542024-542043 None:intergenic 55.0%
CTTCTTTGCCCATGCAGCTG+TGG + chr5_2:542322-542341 None:intergenic 55.0%
CTCGAGACATGGCTAGGCCA+GGG + chr5_2:542018-542037 None:intergenic 60.0%
AGAGGAAGCTGCAAGGGCAG+TGG - chr5_2:542287-542306 Msa0747710:CDS 60.0%
TGGAAGCAGCCACAGCTGCA+TGG - chr5_2:542310-542329 Msa0747710:CDS 60.0%
GGAAGCAGCCACAGCTGCAT+GGG - chr5_2:542311-542330 Msa0747710:CDS 60.0%
CTCGAGACATGGCTAGGCCA+GGG + chr5_2:542018-542037 None:intergenic 60.0%
AGAGGAAGCTGCAAGGGCAG+TGG - chr5_2:542287-542306 Msa0747710:CDS 60.0%
TGGAAGCAGCCACAGCTGCA+TGG - chr5_2:542310-542329 Msa0747710:CDS 60.0%
GGAAGCAGCCACAGCTGCAT+GGG - chr5_2:542311-542330 Msa0747710:CDS 60.0%
GCTCGAGACATGGCTAGGCC+AGG + chr5_2:542019-542038 None:intergenic 65.0%
GGAAGCTGCAAGGGCAGTGG+TGG - chr5_2:542290-542309 Msa0747710:CDS 65.0%
GCTCGAGACATGGCTAGGCC+AGG + chr5_2:542019-542038 None:intergenic 65.0%
GGAAGCTGCAAGGGCAGTGG+TGG - chr5_2:542290-542309 Msa0747710:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 540029 542440 540029 ID=Msa0747710;Name=Msa0747710
chr5_2 mRNA 540029 542440 540029 ID=Msa0747710-mRNA-1;Parent=Msa0747710;Name=Msa0747710-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|5|0|344
chr5_2 exon 542264 542440 542264 ID=Msa0747710-mRNA-1:exon:131;Parent=Msa0747710-mRNA-1
chr5_2 exon 541367 541672 541367 ID=Msa0747710-mRNA-1:exon:130;Parent=Msa0747710-mRNA-1
chr5_2 exon 541168 541282 541168 ID=Msa0747710-mRNA-1:exon:129;Parent=Msa0747710-mRNA-1
chr5_2 exon 540325 540548 540325 ID=Msa0747710-mRNA-1:exon:128;Parent=Msa0747710-mRNA-1
chr5_2 exon 540029 540241 540029 ID=Msa0747710-mRNA-1:exon:127;Parent=Msa0747710-mRNA-1
chr5_2 CDS 542264 542440 542264 ID=Msa0747710-mRNA-1:cds;Parent=Msa0747710-mRNA-1
chr5_2 CDS 541367 541672 541367 ID=Msa0747710-mRNA-1:cds;Parent=Msa0747710-mRNA-1
chr5_2 CDS 541168 541282 541168 ID=Msa0747710-mRNA-1:cds;Parent=Msa0747710-mRNA-1
chr5_2 CDS 540325 540548 540325 ID=Msa0747710-mRNA-1:cds;Parent=Msa0747710-mRNA-1
chr5_2 CDS 540029 540241 540029 ID=Msa0747710-mRNA-1:cds;Parent=Msa0747710-mRNA-1
Gene Sequence

>Msa0747710

ATGGGTATCTGCATATCCGTTGCATCTTCAGAGATTCATGGAATCCCTCAAGTTCATGATGAGAATGTCATGATATTTGAAGCAAGCAAGGTCCAAAATGAGACTAAGAGACTTTGCTCCGTTTACTCTAAACAAGGTACTAAGGGTCTCAACCAAGATGCTGCTTCTCTTCACCAGGATTATGGGATGAAAGATGGAGCATTTTGTGGTGTTTATGATGGACATGGAAAAAATGGACATATAGTGAGCAAAATAGTAAACAATACTTTACCATCTCTCATTTTGAGCCAAAAGAATGCCCTTGAGGAGATTAATACAACAAAAAATGGTGTTGACAACAAACAAAACAAATTTTCAAACAACTATCTAAGATGGAAAGAAGCTATTCTTGGAGCTTTCAATGTAATGGATGAAGAGGTGAAGCAACAAGAGAATCTAGATTGTTCTTGTAGTGGAACTACTGCTGTAGTTGTCATAAGACAGGGTGAAGGTCTTATCATAGTTAACCTTGGTGACTCAAGAGCAATATTAGGAACAATCCAAGATGAAAAACTAAAAGCCATTCAATTGACCACTGATTTGAAGCCGGGATTGCCTTGCGAAGCAAAGAGAATAAGGAGTTGCAACGGTTGTGTTTATGCGTTAAAGGAAGAACCACATGTCCAACGAGTATGGTTGCCCAATGAGAACTACCCTGGCCTAGCCATGTCTCGAGCTTTCGGAGATTTCATACTTAAAGACCATGGTGTTATTGCTACACCAGATATTTGGTATCACCGTTTAACATCAAGTGACCAATTTATTGTTCTTGCAAGTGATGGGGTGTGGGATGTACTAAGTAATGAAGAGGTTGCATCAATTGTGTGGATGGTAGAGAGTGAAGAGGAAGCTGCAAGGGCAGTGGTGGAAGCAGCCACAGCTGCATGGGCAAAGAAGTTTCCTTCCTCTAGGGTAGATGATTGCACCGTGGTTTGCCATTTCCTGCAGAAGAAACCACAAAACTTGGAGTATATGGACAGTGGAAAATTAGGTTAA
Protein sequence

>Msa0747710

MGICISVASSEIHGIPQVHDENVMIFEASKVQNETKRLCSVYSKQGTKGLNQDAASLHQDYGMKDGAFCGVYDGHGKNGHIVSKIVNNTLPSLILSQKNALEEINTTKNGVDNKQNKFSNNYLRWKEAILGAFNVMDEEVKQQENLDCSCSGTTAVVVIRQGEGLIIVNLGDSRAILGTIQDEKLKAIQLTTDLKPGLPCEAKRIRSCNGCVYALKEEPHVQRVWLPNENYPGLAMSRAFGDFILKDHGVIATPDIWYHRLTSSDQFIVLASDGVWDVLSNEEVASIVWMVESEEEAARAVVEAATAAWAKKFPSSRVDDCTVVCHFLQKKPQNLEYMDSGKLG*