AlfalfaGEDB | Msa1172640

M. sativa cultivar zhongmu-4 / Msa1172640

KEGG: K07904
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0012505 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0097708
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172640	XP_013445031.1	98.690	229	3	0	1	229	1	229	5.08e-168	472

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172640	sp\|Q9FE79\|RAA4C_ARATH	57.080	226	91	3	1	226	1	220	3.57e-94	278

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007540	Msa1172640	0.801508	8.712878e-49	-8.615850e-47
Msa0009760	Msa1172640	0.830016	3.722470e-55	-8.615850e-47
Msa0031820	Msa1172640	0.842372	2.692397e-58	-8.615850e-47
Msa0032320	Msa1172640	0.804415	2.184585e-49	-8.615850e-47
Msa0033880	Msa1172640	0.813636	2.315120e-51	-8.615850e-47
Msa0039250	Msa1172640	0.834539	2.827373e-56	-8.615850e-47
Msa0040450	Msa1172640	0.805116	1.558816e-49	-8.615850e-47
Msa0057610	Msa1172640	0.800546	1.370023e-48	-8.615850e-47
Msa0057710	Msa1172640	0.816073	6.674591e-52	-8.615850e-47
Msa0069600	Msa1172640	0.800371	1.487545e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172640	MtrunA17_Chr8g0351861	98.690	229	3	0	1	229	1	229	4.67e-172	472
Msa1172640	MtrunA17_Chr8g0381841	58.371	221	87	3	6	226	8	223	1.04e-92	271
Msa1172640	MtrunA17_Chr3g0123821	57.014	221	89	3	6	226	7	221	1.25e-91	268
Msa1172640	MtrunA17_Chr1g0174281	54.867	226	98	3	1	226	1	222	1.24e-89	263
Msa1172640	MtrunA17_Chr7g0231131	56.604	212	89	2	1	212	1	209	8.61e-89	261
Msa1172640	MtrunA17_Chr2g0319951	52.361	233	89	3	1	227	1	217	3.01e-82	244
Msa1172640	MtrunA17_Chr7g0214241	54.884	215	87	2	15	227	14	220	1.23e-81	243
Msa1172640	MtrunA17_Chr2g0315551	53.304	227	95	4	1	227	1	216	1.34e-81	243
Msa1172640	MtrunA17_Chr4g0032201	53.394	221	93	3	8	228	5	215	4.27e-81	241
Msa1172640	MtrunA17_Chr8g0346421	54.839	217	91	3	10	225	20	230	7.40e-80	239

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172640	AT5G47960.1	57.080	226	91	3	1	226	1	220	3.63e-95	278
Msa1172640	AT3G12160.1	57.965	226	88	3	1	226	1	219	1.16e-94	276
Msa1172640	AT4G39990.1	56.682	217	84	3	13	227	14	222	1.83e-87	258
Msa1172640	AT5G65270.1	55.349	215	94	1	13	227	14	226	1.76e-85	253
Msa1172640	AT4G18430.1	58.947	190	75	1	1	190	1	187	8.71e-83	246
Msa1172640	AT1G16920.1	55.507	227	89	4	1	227	1	215	1.28e-82	246
Msa1172640	AT1G07410.1	55.204	221	88	3	8	228	5	214	1.29e-82	245
Msa1172640	AT2G43130.1	53.540	226	85	3	7	227	3	213	1.83e-82	245
Msa1172640	AT4G18800.1	54.626	227	89	3	1	227	1	213	1.41e-81	243
Msa1172640	AT1G09630.1	55.760	217	88	3	11	227	7	215	1.43e-81	243

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Find 52 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTGAAGATCCAAGGT+GGG	0.619334	8_1:+2767047	Msa1172640:five_prime_UTR
AAACGTACGAGGAAGGATTA+AGG	0.368191	8_1:-2766789	None:intergenic
AAAGACTTAGCAAAACAGAA+AGG	0.614557	8_1:+2768768	Msa1172640:CDS
AAAGTAAATCTTGAATTTGA+GGG	0.497241	8_1:+2768924	Msa1172640:CDS
AAATAACATAGTAACTTCAA+AGG	0.603788	8_1:-2769143	None:intergenic
AAATGAATAAACAGTTATTT+TGG	0.170688	8_1:+2769201	Msa1172640:three_prime_UTR
AACATAGTTGTCATGCTTGT+TGG	0.359266	8_1:+2768696	Msa1172640:CDS
AAGAAACACATCACTGCAGA+TGG	0.556898	8_1:+2768882	Msa1172640:CDS
AAGAGAATGGCTCAGTTTCA+AGG	0.475792	8_1:+2767123	Msa1172640:exon
AAGTCTTTGGCTTCCTCGAT+AGG	0.469086	8_1:-2768753	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTTCAAGGTTGTTATGAT+TGG	+	chr8_1:2767174-2767193	Msa1172640:CDS	30.0%
AATTGTTCTGATTTCAAGCT+TGG	-	chr8_1:2768011-2768030	None:intergenic	30.0%
TTATTTATGATGCAACACAG+TGG	+	chr8_1:2768125-2768144	Msa1172640:intron	30.0%
TATTTATGATGCAACACAGT+GGG	+	chr8_1:2768126-2768145	Msa1172640:intron	30.0%
ACTAACAAAAGACACATTTG+AGG	-	chr8_1:2768318-2768337	None:intergenic	30.0%
CGATTCTGTTAGTAATTCAA+TGG	-	chr8_1:2768350-2768369	None:intergenic	30.0%
AATTACTAACAGAATCGACT+TGG	+	chr8_1:2768353-2768372	Msa1172640:intron	30.0%
GACATGCATAATGTATTTGA+AGG	-	chr8_1:2768507-2768526	None:intergenic	30.0%
AATATGCAGTTGTAATTGCT+TGG	-	chr8_1:2768572-2768591	None:intergenic	30.0%
ATTACAACTGCATATTACAG+AGG	+	chr8_1:2768576-2768595	Msa1172640:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	2766765	2769299	2766765	ID=Msa1172640;Name=Msa1172640
chr8_1	mRNA	2766765	2769299	2766765	ID=Msa1172640-mRNA-1;Parent=Msa1172640;Name=Msa1172640-mRNA-1;_AED=0.03;_eAED=0.03;_QI=364\|1\|1\|1\|0\|1\|2\|274\|229
chr8_1	exon	2766765	2767358	2766765	ID=Msa1172640-mRNA-1:exon:992;Parent=Msa1172640-mRNA-1
chr8_1	exon	2768566	2769299	2768566	ID=Msa1172640-mRNA-1:exon:993;Parent=Msa1172640-mRNA-1
chr8_1	five_prime_UTR	2766765	2767128	2766765	ID=Msa1172640-mRNA-1:five_prime_utr;Parent=Msa1172640-mRNA-1
chr8_1	CDS	2767129	2767358	2767129	ID=Msa1172640-mRNA-1:cds;Parent=Msa1172640-mRNA-1
chr8_1	CDS	2768566	2769025	2768566	ID=Msa1172640-mRNA-1:cds;Parent=Msa1172640-mRNA-1
chr8_1	three_prime_UTR	2769026	2769299	2769026	ID=Msa1172640-mRNA-1:three_prime_utr;Parent=Msa1172640-mRNA-1

Gene Sequence

>Msa1172640

ATGGCTCAGTTTCAAGGTGATTTGGATCATGAGGGTATTGATTATATGTTCAAGGTTGTTATGATTGGTGACTCTGGTGTTGGAAAATCTCAGCTTTTGAATAGGTTTGTTAGAAATGAATTTCATTTGAAATCTAAAGCTACAATTGGTGTTGAGTTTTTGACCAAGACTGTTCTTATGGATCATAAGCTTGTCAAGGCTCAGATTTGGGATACTGCTGGTCAAGAAAGGTACCAAGCAATTACAACTGCATATTACAGAGGTGCAACTGGTGCATTGCTCACATATGACATAACCAAGCGCCATACCTTCAACCATATTGAAAAGTGGCTAGATGAACTACATAACCATGCAGATAAAAACATAGTTGTCATGCTTGTTGGCAACAAGTCTGACCTGAGTTCAATCCGAGAAGTGCCTATCGAGGAAGCCAAAGACTTAGCAAAACAGAAAGGTCTCTTCTTCATTGAGACATCTGCACTCGACTCAAGCAATGTAGAACCAGCTTTCCTTGGTCTTCTCTCACAAATATATGTAACAATGAGTAAGAAACACATCACTGCAGATGGACATGAACGAAATTGGGATAAAGTAAATCTTGAATTTGAGGGAACAAAATTATTGGTCTCGTCGCAAGAACCGGAGTGTCAAAAATCCAAAAAGAGATTCAGTTGTTGCAGTGTTTTTTAG

Protein sequence

>Msa1172640

MAQFQGDLDHEGIDYMFKVVMIGDSGVGKSQLLNRFVRNEFHLKSKATIGVEFLTKTVLMDHKLVKAQIWDTAGQERYQAITTAYYRGATGALLTYDITKRHTFNHIEKWLDELHNHADKNIVVMLVGNKSDLSSIREVPIEEAKDLAKQKGLFFIETSALDSSNVEPAFLGLLSQIYVTMSKKHITADGHERNWDKVNLEFEGTKLLVSSQEPECQKSKKRFSCCSVF*