AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44017


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44017.t1 MTR_1g081280 90.071 141 14 0 1 141 1 141 4.51e-91 264
MS.gene44017.t1 MTR_1g081280 90.071 141 14 0 1 141 1 141 4.46e-90 262
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44017.t1 AT3G04890 50.000 84 42 0 57 140 33 116 1.38e-23 92.0
MS.gene44017.t1 AT3G04890 50.000 84 42 0 57 140 33 116 1.43e-23 91.7
MS.gene44017.t1 AT3G04890 50.000 84 42 0 57 140 37 120 1.88e-23 91.7
MS.gene44017.t1 AT3G04890 50.000 84 42 0 57 140 37 120 2.08e-23 91.7

Find 28 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATCATCAATACTTGAAAC+TGG 0.314009 1.3:+57480384 None:intergenic
GATCCAACTATTAAATTTAG+TGG 0.322543 1.3:-57480103 MS.gene44017:intron
GCAAAAGTCTTTCTGCTACT+TGG 0.371862 1.3:+57480851 None:intergenic
TTACCACTAAATTTAATAGT+TGG 0.387007 1.3:+57480100 None:intergenic
TGATGTTCTAATCATCATAA+AGG 0.421681 1.3:-57480367 MS.gene44017:CDS
TGGAAGTGTAAGGCTATTGT+AGG 0.430972 1.3:+57481049 None:intergenic
AGATACCTTGTTTGTTGAGG+AGG 0.500425 1.3:+57480737 None:intergenic
CATAGATACCTTGTTTGTTG+AGG 0.512642 1.3:+57480734 None:intergenic
GGAAGTGTAAGGCTATTGTA+GGG 0.518951 1.3:+57481050 None:intergenic
GAGGAGGAGAGAAAAGCCTC+AGG 0.522489 1.3:+57480753 None:intergenic
GGACGCCACCCTTGCTGTTG+CGG 0.524042 1.3:-57480901 MS.gene44017:CDS
AGAAAATAACGAAACCCCTC+AGG 0.529785 1.3:-57480810 MS.gene44017:CDS
AGACATTTATTACCTGGGAG+TGG 0.539869 1.3:+57481029 None:intergenic
CATCATCAATACTTGAAACT+GGG 0.541779 1.3:+57480385 None:intergenic
GCGGAGTGACGGAGCTCCTG+AGG 0.554237 1.3:-57480769 MS.gene44017:CDS
GATTGCAGTGAGCGGAGTGA+CGG 0.565614 1.3:-57480780 MS.gene44017:CDS
AGCCTTACACTTCCACTCCC+AGG 0.566168 1.3:-57481041 MS.gene44017:intron
TGTTTCCGCAACAGCAAGGG+TGG 0.575559 1.3:+57480896 None:intergenic
CTGCAATCTTCAGAACCTGA+GGG 0.576531 1.3:+57480795 None:intergenic
TCTCTCCTCCTCAACAAACA+AGG 0.582792 1.3:-57480742 MS.gene44017:intron
TGTTGTTTCCGCAACAGCAA+GGG 0.587584 1.3:+57480893 None:intergenic
GTGTTGTTTCCGCAACAGCA+AGG 0.594278 1.3:+57480892 None:intergenic
TACCTGGGAGTGGAAGTGTA+AGG 0.601591 1.3:+57481039 None:intergenic
ACTGCAATCTTCAGAACCTG+AGG 0.643213 1.3:+57480794 None:intergenic
ATCATCAATACTTGAAACTG+GGG 0.643651 1.3:+57480386 None:intergenic
GTTCTGAAGATTGCAGTGAG+CGG 0.663984 1.3:-57480788 MS.gene44017:CDS
TGCAATCTTCAGAACCTGAG+GGG 0.670652 1.3:+57480796 None:intergenic
GAAGTGTAAGGCTATTGTAG+GGG 0.673629 1.3:+57481051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAAAAATATTTGATAAG+AGG + chr1.3:57480760-57480779 None:intergenic 15.0%
!! GTAATTTAAGAAAATAAAAG+TGG + chr1.3:57480442-57480461 None:intergenic 15.0%
!! TGAAAAAATATTTGATAAGA+GGG + chr1.3:57480759-57480778 None:intergenic 15.0%
!! AACACTGCATATTAATAAAT+TGG + chr1.3:57480599-57480618 None:intergenic 20.0%
!! AATAAAGACATTTATTACCT+GGG + chr1.3:57480018-57480037 None:intergenic 20.0%
!! TTACCACTAAATTTAATAGT+TGG + chr1.3:57480942-57480961 None:intergenic 20.0%
!!! TTAAAGATTTGAAACATCTA+GGG + chr1.3:57481085-57481104 None:intergenic 20.0%
! AGACTAGCATAAGTTATTAT+AGG + chr1.3:57480980-57480999 None:intergenic 25.0%
! GAATAAAGACATTTATTACC+TGG + chr1.3:57480019-57480038 None:intergenic 25.0%
! GATCCAACTATTAAATTTAG+TGG - chr1.3:57480936-57480955 MS.gene44017:intron 25.0%
! TGATGTTCTAATCATCATAA+AGG - chr1.3:57480672-57480691 MS.gene44017:intron 25.0%
!! CTTACTTTGTTTGAGATTTA+GGG - chr1.3:57480871-57480890 MS.gene44017:CDS 25.0%
!! GACTTTTGCAATTTATAACA+TGG - chr1.3:57480202-57480221 MS.gene44017:intron 25.0%
!! TCTTACTTTGTTTGAGATTT+AGG - chr1.3:57480870-57480889 MS.gene44017:CDS 25.0%
ACATCATCAATACTTGAAAC+TGG + chr1.3:57480658-57480677 None:intergenic 30.0%
ATCATCAATACTTGAAACTG+GGG + chr1.3:57480656-57480675 None:intergenic 30.0%
CATCATCAATACTTGAAACT+GGG + chr1.3:57480657-57480676 None:intergenic 30.0%
TTTAGATTACATGCCAGAAA+AGG + chr1.3:57480846-57480865 None:intergenic 30.0%
! TTCAACATCATTTCCTTTTC+TGG - chr1.3:57480830-57480849 MS.gene44017:CDS 30.0%
CAACATTGACGCTTAAACTT+TGG + chr1.3:57480396-57480415 None:intergenic 35.0%
CTGAATCTCTTGTTAAGACT+AGG - chr1.3:57480117-57480136 MS.gene44017:CDS 35.0%
! CATAGATACCTTGTTTGTTG+AGG + chr1.3:57480308-57480327 None:intergenic 35.0%
! TCAGTCTGAAGTGGAATAAA+TGG + chr1.3:57480331-57480350 None:intergenic 35.0%
AAGATGTCTTCAGTCTGAAG+TGG + chr1.3:57480340-57480359 None:intergenic 40.0%
ACACATACCAATGTCGACAT+TGG - chr1.3:57480370-57480389 MS.gene44017:CDS 40.0%
AGAAAATAACGAAACCCCTC+AGG - chr1.3:57480229-57480248 MS.gene44017:intron 40.0%
AGACATTTATTACCTGGGAG+TGG + chr1.3:57480013-57480032 None:intergenic 40.0%
ATTACTACCAGTGTCATGTC+TGG - chr1.3:57480457-57480476 MS.gene44017:intron 40.0%
CTTCAGACTGAAGACATCTT+CGG - chr1.3:57480340-57480359 MS.gene44017:CDS 40.0%
GAAGTGTAAGGCTATTGTAG+GGG + chr1.3:57479991-57480010 None:intergenic 40.0%
GCAAAAGTCTTTCTGCTACT+TGG + chr1.3:57480191-57480210 None:intergenic 40.0%
! AGATACCTTGTTTGTTGAGG+AGG + chr1.3:57480305-57480324 None:intergenic 40.0%
! GCAAAAACATGCTGCTTCAA+CGG - chr1.3:57479960-57479979 MS.gene44017:intron 40.0%
! GCATAAGTTGACGATTAGTG+AGG + chr1.3:57480548-57480567 None:intergenic 40.0%
! GGAAGTGTAAGGCTATTGTA+GGG + chr1.3:57479992-57480011 None:intergenic 40.0%
! TGGAAGTGTAAGGCTATTGT+AGG + chr1.3:57479993-57480012 None:intergenic 40.0%
ACTGCAATCTTCAGAACCTG+AGG + chr1.3:57480248-57480267 None:intergenic 45.0%
ACTTTGGCCAATGTCGACAT+TGG + chr1.3:57480380-57480399 None:intergenic 45.0%
ATGCATATTTCCCTGGCAGT+TGG + chr1.3:57480524-57480543 None:intergenic 45.0%
CTGCAATCTTCAGAACCTGA+GGG + chr1.3:57480247-57480266 None:intergenic 45.0%
GGTGTATGTGTAAGAGGTAG+TGG - chr1.3:57480483-57480502 MS.gene44017:intron 45.0%
GTGAGGAATGCATATTTCCC+TGG + chr1.3:57480531-57480550 None:intergenic 45.0%
TCTCTCCTCCTCAACAAACA+AGG - chr1.3:57480297-57480316 MS.gene44017:intron 45.0%
TGCAATCTTCAGAACCTGAG+GGG + chr1.3:57480246-57480265 None:intergenic 45.0%
TGGTCTGGTGTATGTGTAAG+AGG - chr1.3:57480477-57480496 MS.gene44017:intron 45.0%
! GAGGTAGTGGTTCATGTTGT+TGG - chr1.3:57480496-57480515 MS.gene44017:intron 45.0%
!! GTTCTGAAGATTGCAGTGAG+CGG - chr1.3:57480251-57480270 MS.gene44017:intron 45.0%
!! TGTTGTTTCCGCAACAGCAA+GGG + chr1.3:57480149-57480168 None:intergenic 45.0%
!!! GACAGCGCTTATTTTGTCAC+AGG - chr1.3:57480703-57480722 MS.gene44017:intron 45.0%
GTTGTTGGAACCAACTGCCA+GGG - chr1.3:57480511-57480530 MS.gene44017:intron 50.0%
TACCAGTGTCATGTCTGGTC+TGG - chr1.3:57480462-57480481 MS.gene44017:intron 50.0%
TGTTGTTGGAACCAACTGCC+AGG - chr1.3:57480510-57480529 MS.gene44017:intron 50.0%
! TACCTGGGAGTGGAAGTGTA+AGG + chr1.3:57480003-57480022 None:intergenic 50.0%
!! GTGTTGTTTCCGCAACAGCA+AGG + chr1.3:57480150-57480169 None:intergenic 50.0%
AGCCTTACACTTCCACTCCC+AGG - chr1.3:57479998-57480017 MS.gene44017:intron 55.0%
CACCAGACCAGACATGACAC+TGG + chr1.3:57480467-57480486 None:intergenic 55.0%
GAGGAGGAGAGAAAAGCCTC+AGG + chr1.3:57480289-57480308 None:intergenic 55.0%
GATTGCAGTGAGCGGAGTGA+CGG - chr1.3:57480259-57480278 MS.gene44017:intron 55.0%
TGTTTCCGCAACAGCAAGGG+TGG + chr1.3:57480146-57480165 None:intergenic 55.0%
!! GGACGCCACCCTTGCTGTTG+CGG - chr1.3:57480138-57480157 MS.gene44017:CDS 65.0%
! GCGGAGTGACGGAGCTCCTG+AGG - chr1.3:57480270-57480289 MS.gene44017:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 57479957 57481104 57479957 ID=MS.gene44017
chr1.3 mRNA 57479957 57481104 57479957 ID=MS.gene44017.t1;Parent=MS.gene44017
chr1.3 exon 57481042 57481104 57481042 ID=MS.gene44017.t1.exon1;Parent=MS.gene44017.t1
chr1.3 CDS 57481042 57481104 57481042 ID=cds.MS.gene44017.t1;Parent=MS.gene44017.t1
chr1.3 exon 57480743 57480927 57480743 ID=MS.gene44017.t1.exon2;Parent=MS.gene44017.t1
chr1.3 CDS 57480743 57480927 57480743 ID=cds.MS.gene44017.t1;Parent=MS.gene44017.t1
chr1.3 exon 57480337 57480446 57480337 ID=MS.gene44017.t1.exon3;Parent=MS.gene44017.t1
chr1.3 CDS 57480337 57480446 57480337 ID=cds.MS.gene44017.t1;Parent=MS.gene44017.t1
chr1.3 exon 57480104 57480169 57480104 ID=MS.gene44017.t1.exon4;Parent=MS.gene44017.t1
chr1.3 CDS 57480104 57480169 57480104 ID=cds.MS.gene44017.t1;Parent=MS.gene44017.t1
chr1.3 exon 57479957 57479973 57479957 ID=MS.gene44017.t1.exon5;Parent=MS.gene44017.t1
chr1.3 CDS 57479957 57479973 57479957 ID=cds.MS.gene44017.t1;Parent=MS.gene44017.t1
Gene Sequence

>MS.gene44017

ATGGCAAAAACATGCTGCTTCAACGGAAAATCCCCTACAATAGCCTTACACTTCCACTCCCAGGTAATAAATGTCTTTATTCTGAGATGAGTTATTTATTTGTAAAGCGATCAAAGTGAATTATTTATATGAAGAAGTAAATTCTGATAATTAATGTTCACTGAATCTCTTGTTAAGACTAGGACGCCACCCTTGCTGTTGCGGAAACAACACTGCTCAAAAAGTTACACACCAAGTAGCAGAAAGACTTTTGCAATTTATAACATGGTAAGAGAAAATAACGAAACCCCTCAGGTTCTGAAGATTGCAGTGAGCGGAGTGACGGAGCTCCTGAGGCTTTTCTCTCCTCCTCAACAAACAAGGTATCTATGCCATTTATTCCACTTCAGACTGAAGACATCTTCGGTATTCAAACACATACCAATGTCGACATTGGCCAAAGTTTAAGCGTCAATGTTGTGTCTAGTATCTGTATGTGTCAACCACTTTTATTTTCTTAAATTACTACCAGTGTCATGTCTGGTCTGGTGTATGTGTAAGAGGTAGTGGTTCATGTTGTTGGAACCAACTGCCAGGGAAATATGCATTCCTCACTAATCGTCAACTTATGCGTTTATTAATCGTTGAGTGAATCATACTCCAATTTATTAATATGCAGTGTTTTAAGCAGTAACGTTGAGAAACAGAATAATGACTCCCCAGTTTCAAGTATTGATGATGTTCTAATCATCATAAAGGATGATTATGACAGCGCTTATTTTGTCACAGGTAGCTAGCTTGTTTTTCTTTCTTTCAATTTCCCTCTTATCAAATATTTTTTCATGACATTGCTCTTACTTGCTAATTTAGCATCTTTTTGCTATTTGTTTTTAATTCAACATCATTTCCTTTTCTGGCATGTAATCTAAAATTGTCTTACTTTGTTTGAGATTTAGGGAACTTCACTTCTTCAATTTACACAGAAAATTGCATCTTTGAAGATCCAACTATTAAATTTAGTGGTAAGAATCATGACTTTTACCTATAATAACTTATGCTAGTCTTTCTGTCGCGAATTTTTAATTGATCTTCTTCATCATCAATTTAATTGATCAATAACTAAAAGTTACACATTGCTCTTGTTCTCCCTAGATGTTTCAAATCTTTAA

Protein sequence

>MS.gene44017.t1

MAKTCCFNGKSPTIALHFHSQTRTPPLLLRKQHCSKSYTPSSRKTFAIYNMVRENNETPQVLKIAVSGVTELLRLFSPPQQTSVLSSNVEKQNNDSPVSSIDDVLIIIKDDYDSAYFVTGNFTSSIYTENCIFEDPTIKFSDVSNL